LOCUS       DACCZY010000001        60319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCZY010000001 DACCZY010000000
VERSION     DACCZY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.001"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1421)
                     /locus_tag="IVZ67_000001"
     CDS             complement(<1..1421)
                     /locus_tag="IVZ67_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8164110.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="IVZ67_000002"
     CDS             1673..2854
                     /locus_tag="IVZ67_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8164111.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="IVZ67_000003"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="IVZ67_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8164112.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="IVZ67_000004"
     CDS             complement(4993..7704)
                     /locus_tag="IVZ67_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8164113.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="IVZ67_000005"
     CDS             complement(7714..8415)
                     /locus_tag="IVZ67_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164114.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="IVZ67_000006"
     CDS             8601..9200
                     /locus_tag="IVZ67_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8164115.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="IVZ67_000007"
     CDS             9281..10294
                     /locus_tag="IVZ67_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164116.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="IVZ67_000008"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="IVZ67_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8164117.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="IVZ67_000009"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="IVZ67_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8164118.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="IVZ67_000010"
     CDS             11742..13115
                     /locus_tag="IVZ67_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164119.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="IVZ67_000011"
     CDS             13472..15616
                     /locus_tag="IVZ67_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8164120.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="IVZ67_000012"
     CDS             complement(15668..16708)
                     /locus_tag="IVZ67_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8164121.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="IVZ67_000013"
     CDS             complement(16985..18787)
                     /locus_tag="IVZ67_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164122.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="IVZ67_000014"
     CDS             complement(18801..19553)
                     /locus_tag="IVZ67_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164123.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="IVZ67_000015"
     CDS             complement(19562..19924)
                     /locus_tag="IVZ67_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8164124.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="IVZ67_000016"
     CDS             complement(20100..20972)
                     /locus_tag="IVZ67_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8164125.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="IVZ67_000017"
     CDS             complement(21023..21511)
                     /locus_tag="IVZ67_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8164126.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22479)
                     /locus_tag="IVZ67_000018"
     CDS             complement(21649..22479)
                     /locus_tag="IVZ67_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8164127.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22495..23103)
                     /locus_tag="IVZ67_000019"
     CDS             complement(22495..23103)
                     /locus_tag="IVZ67_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8164128.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="IVZ67_000020"
     CDS             complement(23096..23413)
                     /locus_tag="IVZ67_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8164129.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="IVZ67_000021"
     CDS             complement(23569..24297)
                     /locus_tag="IVZ67_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8164130.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="IVZ67_000022"
     CDS             24456..25337
                     /locus_tag="IVZ67_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8164131.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="IVZ67_000023"
     CDS             complement(25574..26617)
                     /locus_tag="IVZ67_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8164132.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="IVZ67_000024"
     CDS             complement(26620..27288)
                     /locus_tag="IVZ67_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8164133.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="IVZ67_000025"
     CDS             27430..29766
                     /locus_tag="IVZ67_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8164134.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="IVZ67_000026"
     CDS             complement(29881..30192)
                     /locus_tag="IVZ67_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164135.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="IVZ67_000027"
     CDS             30373..31848
                     /locus_tag="IVZ67_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8164136.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="IVZ67_000028"
     CDS             31895..32479
                     /locus_tag="IVZ67_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8164137.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="IVZ67_000029"
     CDS             32481..32864
                     /locus_tag="IVZ67_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8164138.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="IVZ67_000030"
     CDS             complement(33181..33465)
                     /locus_tag="IVZ67_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8164139.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="IVZ67_000031"
     CDS             complement(33817..34527)
                     /locus_tag="IVZ67_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8164140.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="IVZ67_000032"
     CDS             complement(34524..35996)
                     /locus_tag="IVZ67_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8164141.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="IVZ67_000033"
     CDS             36134..36607
                     /locus_tag="IVZ67_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8164142.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="IVZ67_000034"
     CDS             complement(36760..37311)
                     /locus_tag="IVZ67_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8164143.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="IVZ67_000035"
     CDS             37416..37946
                     /locus_tag="IVZ67_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8164144.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="IVZ67_000036"
     CDS             complement(38429..38872)
                     /locus_tag="IVZ67_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164145.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="IVZ67_000037"
     CDS             complement(38888..40048)
                     /locus_tag="IVZ67_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8164146.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="IVZ67_000038"
     CDS             40555..42255
                     /locus_tag="IVZ67_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164147.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="IVZ67_000039"
     CDS             complement(42357..43190)
                     /locus_tag="IVZ67_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8164148.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="IVZ67_000040"
     CDS             complement(43494..45659)
                     /locus_tag="IVZ67_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164149.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="IVZ67_000041"
     CDS             45919..48213
                     /locus_tag="IVZ67_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8164150.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="IVZ67_000042"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="IVZ67_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8164151.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="IVZ67_000043"
     CDS             48864..49871
                     /locus_tag="IVZ67_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8164152.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="IVZ67_000044"
     CDS             49893..50912
                     /locus_tag="IVZ67_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164153.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="IVZ67_000045"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="IVZ67_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8164154.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /locus_tag="IVZ67_000046"
     CDS             complement(52669..54090)
                     /locus_tag="IVZ67_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8164155.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="IVZ67_000047"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="IVZ67_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8164156.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /locus_tag="IVZ67_000048"
     CDS             complement(56501..57757)
                     /locus_tag="IVZ67_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8164157.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58337..59638
                     /locus_tag="IVZ67_000049"
     CDS             58337..59638
                     /locus_tag="IVZ67_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8164158.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="IVZ67_000050"
     CDS             complement(59707..60306)
                     /locus_tag="IVZ67_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8164159.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18206 a        12347 c        10816 g        18950 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctcc
//
LOCUS       DACCZY010000002        59782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCZY010000002 DACCZY010000000
VERSION     DACCZY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.002"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            279..547
                     /locus_tag="IVZ67_000051"
                     /pseudo
     CDS             279..547
                     /locus_tag="IVZ67_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1219..4340
                     /locus_tag="IVZ67_000052"
                     /pseudo
     CDS             1219..4340
                     /locus_tag="IVZ67_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(4764..4934)
                     /locus_tag="IVZ67_000053"
     CDS             complement(4764..4934)
                     /locus_tag="IVZ67_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8164160.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5128..5307)
                     /locus_tag="IVZ67_000054"
     CDS             complement(5128..5307)
                     /locus_tag="IVZ67_000054"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164161.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5694..6806
                     /locus_tag="IVZ67_000055"
     CDS             5694..6806
                     /locus_tag="IVZ67_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8164162.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(6884..7177)
                     /locus_tag="IVZ67_000056"
     CDS             complement(6884..7177)
                     /locus_tag="IVZ67_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8164163.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7345..7659
                     /locus_tag="IVZ67_000057"
     CDS             7345..7659
                     /locus_tag="IVZ67_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8164164.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7680..9659
                     /locus_tag="IVZ67_000058"
     CDS             7680..9659
                     /locus_tag="IVZ67_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8164165.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9672..9998
                     /locus_tag="IVZ67_000059"
     CDS             9672..9998
                     /locus_tag="IVZ67_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8164166.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10014..10532
                     /locus_tag="IVZ67_000060"
     CDS             10014..10532
                     /locus_tag="IVZ67_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164167.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10532..11884
                     /gene="celB"
                     /locus_tag="IVZ67_000061"
     CDS             10532..11884
                     /gene="celB"
                     /locus_tag="IVZ67_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8164168.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(11968..13473)
                     /locus_tag="IVZ67_000062"
     CDS             complement(11968..13473)
                     /locus_tag="IVZ67_000062"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8164169.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13565..14155)
                     /locus_tag="IVZ67_000063"
     CDS             complement(13565..14155)
                     /locus_tag="IVZ67_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8164170.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14334..15980
                     /locus_tag="IVZ67_000064"
     CDS             14334..15980
                     /locus_tag="IVZ67_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8164171.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16029..16748
                     /locus_tag="IVZ67_000065"
     CDS             16029..16748
                     /locus_tag="IVZ67_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8164172.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17227..18174
                     /locus_tag="IVZ67_000066"
     CDS             17227..18174
                     /locus_tag="IVZ67_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8164173.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18186..19106
                     /locus_tag="IVZ67_000067"
     CDS             18186..19106
                     /locus_tag="IVZ67_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164174.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19147..20604
                     /locus_tag="IVZ67_000068"
     CDS             19147..20604
                     /locus_tag="IVZ67_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8164175.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            20949..21563
                     /locus_tag="IVZ67_000069"
     CDS             20949..21563
                     /locus_tag="IVZ67_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8164176.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21556..23280
                     /locus_tag="IVZ67_000070"
     CDS             21556..23280
                     /locus_tag="IVZ67_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8164177.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23277..24728
                     /locus_tag="IVZ67_000071"
     CDS             23277..24728
                     /locus_tag="IVZ67_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8164178.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25201..27354)
                     /locus_tag="IVZ67_000072"
     CDS             complement(25201..27354)
                     /locus_tag="IVZ67_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8164179.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27356..28402)
                     /locus_tag="IVZ67_000073"
     CDS             complement(27356..28402)
                     /locus_tag="IVZ67_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8164180.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28552..29841
                     /locus_tag="IVZ67_000074"
     CDS             28552..29841
                     /locus_tag="IVZ67_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8164181.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            29854..32556
                     /locus_tag="IVZ67_000075"
     CDS             29854..32556
                     /locus_tag="IVZ67_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8164182.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32541..33398
                     /locus_tag="IVZ67_000076"
     CDS             32541..33398
                     /locus_tag="IVZ67_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8164183.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33469..34326)
                     /locus_tag="IVZ67_000077"
     CDS             complement(33469..34326)
                     /locus_tag="IVZ67_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8164184.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34537..36708
                     /locus_tag="IVZ67_000078"
     CDS             34537..36708
                     /locus_tag="IVZ67_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8164185.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            36776..37117
                     /locus_tag="IVZ67_000079"
     CDS             36776..37117
                     /locus_tag="IVZ67_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8164186.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37393..39147)
                     /locus_tag="IVZ67_000080"
     CDS             complement(37393..39147)
                     /locus_tag="IVZ67_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164187.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39158..39994)
                     /locus_tag="IVZ67_000081"
     CDS             complement(39158..39994)
                     /locus_tag="IVZ67_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8164188.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(39996..40679)
                     /locus_tag="IVZ67_000082"
     CDS             complement(39996..40679)
                     /locus_tag="IVZ67_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8164189.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(40838..42232)
                     /gene="uxaC"
                     /locus_tag="IVZ67_000083"
     CDS             complement(40838..42232)
                     /gene="uxaC"
                     /locus_tag="IVZ67_000083"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8164190.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(42271..43770)
                     /locus_tag="IVZ67_000084"
     CDS             complement(42271..43770)
                     /locus_tag="IVZ67_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8164191.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(43763..45184)
                     /locus_tag="IVZ67_000085"
     CDS             complement(43763..45184)
                     /locus_tag="IVZ67_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8164192.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(45244..46236)
                     /locus_tag="IVZ67_000086"
     CDS             complement(45244..46236)
                     /locus_tag="IVZ67_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164193.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            46468..48666
                     /locus_tag="IVZ67_000087"
     CDS             46468..48666
                     /locus_tag="IVZ67_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8164194.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(49124..49996)
                     /locus_tag="IVZ67_000088"
     CDS             complement(49124..49996)
                     /locus_tag="IVZ67_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164195.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            50296..51269
                     /locus_tag="IVZ67_000089"
                     /pseudo
     CDS             50296..51269
                     /locus_tag="IVZ67_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            51291..51983
                     /locus_tag="IVZ67_000090"
     CDS             51291..51983
                     /locus_tag="IVZ67_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8164196.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            51949..53457
                     /locus_tag="IVZ67_000091"
     CDS             51949..53457
                     /locus_tag="IVZ67_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8164197.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            53531..54634
                     /locus_tag="IVZ67_000092"
     CDS             53531..54634
                     /locus_tag="IVZ67_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8164198.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            54637..55974
                     /locus_tag="IVZ67_000093"
     CDS             54637..55974
                     /locus_tag="IVZ67_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8164199.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            55961..56839
                     /locus_tag="IVZ67_000094"
     CDS             55961..56839
                     /locus_tag="IVZ67_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8164200.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            56836..57759
                     /locus_tag="IVZ67_000095"
     CDS             56836..57759
                     /locus_tag="IVZ67_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8164201.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            57756..58166
                     /locus_tag="IVZ67_000096"
     CDS             57756..58166
                     /locus_tag="IVZ67_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8164202.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            58170..59738
                     /locus_tag="IVZ67_000097"
     CDS             58170..59738
                     /locus_tag="IVZ67_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8164203.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT        19292 a        10468 c        12279 g        17743 t
ORIGIN      
        1 atttttcgtc gttttataaa aatgttattt tttggaaaaa ttcacaaata tgtaacgaat
       61 gtttttctgg aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt
      121 tatgcaattt tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg
      181 aaaaatagtt gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat
      241 tcgagagaat catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag
      301 atagaattac caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc
      361 gcttcggtta tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat
      421 tgacattaca gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt
      481 acaaggaaac cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac
      541 aagttaagaa agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa
      601 atgcaagaaa tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca
      661 tccatgacga gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat
      721 atggccaaat aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt
      781 ttgatgctaa gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggact
      841 cactgatgaa attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg
      901 aaaagtataa aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt
      961 gtgcaaggca ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa
     1021 ctaaaagtca tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt
     1081 tgtttacagc ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag
     1141 ccattttgag ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa
     1201 ttataagggg gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt
     1261 atgcttattg tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa
     1321 cagccagagc gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag
     1381 ttccagttgc gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt
     1441 gaggatttga ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat
     1501 tcttcccagc attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat
     1561 acggtctcaa tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca
     1621 gaagaaagca caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat
     1681 gaagccagat catctgagcc atctataacg caggaatctc agattgaaag acaagaggag
     1741 ctcttgaatt atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca
     1801 caaacgttaa agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag
     1861 atggtcgtac gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc
     1921 gctgatgatt cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt
     1981 ccatttacct ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc
     2041 acgtttgaag ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg
     2101 agtcttactt atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca
     2161 tcaagaccca ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat
     2221 cttgggaatc tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag
     2281 gtaaattatg aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga
     2341 acaactttgg caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca
     2401 ggtggggttc ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat
     2461 gtttccgagg aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg
     2521 gactttggta atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc
     2581 gatgtcgact tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt
     2641 atgacctaca ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat
     2701 cttccggcaa tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat
     2761 gaactaacgt atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt
     2821 gaagatgttt tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc
     2881 attgatgccg aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt
     2941 ccgacaagtt ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa
     3001 attttaaact ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga
     3061 acaaggaaaa tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg
     3121 gcaggagcaa aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg
     3181 gcagaaaacg gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa
     3241 acagaagcac cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa
     3301 tactccatag aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa
     3361 ctaattaaac atgctgaagg cagtggtgaa gtccttcaag gagctgtttt tgagttacag
     3421 aaccgagaag gagaaacgct acaaaccggt ttaactacag gagaagatgg aaaactggca
     3481 attgacggtt tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aatagggtat
     3541 gaattagatg cgacaccaat cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa
     3601 ttgacgaaag aaaaccgact tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa
     3661 agcggggaaa tcctgcaagg agctgttttt gagttacaga accgagaagg agaaacgcta
     3721 caaaccggtc taactacagg agaagatgga aaactggcaa ttgacggttt agcaccagga
     3781 gcatatcagc tggtagaaac tcaagcgcca atagggtatg aattagatgc gacaccaatc
     3841 gagtttgaga tcgaaagaag tcagacagcg gtggtagaat taacgaaaga aaaccgactt
     3901 acaccaggcg gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaaat cctgcaggag
     3961 ctgtttttga gttacagaac cgagaaggag aaacgctaca aaccggtcta actacaggag
     4021 aagatggaaa actggcaatt gacggtttag caccaggagc atatcagctg gtagaaactc
     4081 aagcgccaat agggtatgaa ttggatgcga caccagtaac tttcaagatc gaaaaagaac
     4141 aagaagtggc gatcttttta acaaaagaga atagaaaagt tgctgatagc tcagatataa
     4201 gaggaaacaa tccaacaagc agtggaaata agcatcttcc aaaaactgga gaaaccatca
     4261 ttagccagtt tatattgtca atattgggtg tgctacttgt tttcatcagt ataaaattta
     4321 gaaagaaacg aaatatatga gctaaaataa aaaattgaat aagcatagtt tttaaaatca
     4381 actagtaccc atcaattgaa tggaaactac cgattttaga aggggtatac aaacaaatat
     4441 tagtatcgga gtagagaagg ttgaacccaa ccagtaaata agtaaagaca attttgtgct
     4501 gcgttgagaa gtgaaaaata aacctttgta gtaatactag ttaataaaaa atatacaaaa
     4561 acactgcgaa aatcattctt gagtttcgca gtgttttttg cctaatgact caagtttatt
     4621 gggtaatact ttgattttct ttctacaaat aatcagcgat cagttgtaat tttttctcta
     4681 gttttaatgg ctctttgtca ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc
     4741 ttcaaccaac tcgtgaataa aggttacgcc tccttgaaag gcgtggaaaa accaagtaat
     4801 cgatcaatca ccaatttcgc atttggtaga acatgtttgg tgagctgaaa gtaggtggcg
     4861 ttcatgtctg taactaagaa ttctatttct ttaggattag tgcagctaag gaaatagttt
     4921 gttaatcgag gaattttttg tgtaggtaaa ttttctcgat taatttttct gtctcgccat
     4981 ccgcggaaat aaaactcagt ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct
     5041 caacactcaa gacagaattc ttttggaaga ctttggtaaa tagcttctca actctttcaa
     5101 tgtgcgaata acggttgtca aagataccta acaacttttc gaaataaaaa ataacgacac
     5161 tttttcagtc agtaaagaag tgattttatg tctgacatga tttgaaatcg agtgccttgg
     5221 acggataaaa taactttgag cgttccaatg gcttcgacag tttttacagg tatagcgctg
     5281 cttttttagg cgtataacca ttggcatatg attatattat tcaaagtgga caatggtttc
     5341 cttcttcttg tttttcacca ccattaattt cctatttcca tctaccacag cagaaccaca
     5401 atttctacag acacatggag cagggaagag aactgcagca acgatcaatg tttttttctt
     5461 ctgtaaggtt tcgtaagaga cctctgtaat catcaaattt ttgtctattg atctcagaat
     5521 ttttttgata gaaccattca taaagcatat cgttctctca gttgtttatt ttgtggtgat
     5581 ttaatcatac aagaaaacga tatgtttttt tataaaaaca tgaataggaa aaatagttga
     5641 caagaaaatg acaacgccat atgatgttcc atagtattta caacctgtca tagatgactg
     5701 taaaaaataa aagtaaaaag aaaggacgac aacaggtgga tttttatcag tctctacaac
     5761 tagatccgtt tattttaaaa caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca
     5821 tgtatatttg ttcattgttt ttacgatcat tattcatcgt attgtttgcc atttgtttta
     5881 tcattataat tacaacatta tttgaatcga agcataaacc atacgctgtg gttttattct
     5941 gtatgttgat gagtattcgt tttgttgact ttggttataa gatttcccat tcgatcatag
     6001 gtttagcaat cgtcatgttc tcactattag tagcaccgta tgtccagctg atcaaatggt
     6061 cagttatggg catgttgatt cattttattt tactaagcag catattaatg gcaaccgcta
     6121 gtgatcccaa aatggggaat gcttctttgt acggcttttc atatctcttt attgtttatt
     6181 cgttacctaa agattcgttg aacaagaatt tctttacaca aacaagtagc ttattatttt
     6241 tgtttttttg ttggttttca gttctccttt acagaaaaca tcgagaaaag aacaaagaca
     6301 aaagcttgtt taaagagatt tttttgaaag gcatgtattc gcaggaaaaa atttggatgc
     6361 tcatctatgc atttggcatt agtcttttga tcgttgcagg agagtatgtc ccttttcagc
     6421 gattgatgtg ggcagggttt gcattttctt ctattgtatc tagctatgga ttgatgtcga
     6481 ttggtttcaa agaacgagca gtagaccgaa tcattggctc attgattgga tgtgttttat
     6541 ttattggtat cagccaattt attcctttta actgggtcgg tatattaggt ggtctagctt
     6601 taggtgtttg ttctacctat agatataaaa cagtttttaa ttcttttggt gctttggcaa
     6661 tcactgccag tttattcggg gttccaggag cagtaacgat ccgcattttt gagaatatcc
     6721 ttggggtatg tttaggaatc atgtatattg gggtgactga aatacttatc cgaaaaataa
     6781 gagaaaaaca cggtttgaat cattagatga gatcgaataa aaaataagga aaaagtgaag
     6841 atacacatgg atgataaatt gctatcattc catgtgtatc tttttacggt aaatcagtga
     6901 aacgttcgac tttcattttt aatttatact ttttacggag ttcgtttatt tgagccatca
     6961 tttcggattt gtgatgcttt tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa
     7021 gagattctgg atcttctagt ggaaagtaat agtcatattt ctcgtttcct tcttctgcac
     7081 gaatcgcttt gacaatgttt ctttcgacca tttcttctgc aaattttctg gcttgtccat
     7141 ttgtacttgt atacaaaata ttcactgtga tactcactaa atcccctcct gaaaaaattc
     7201 tatcactagt atatcacggt gccagaaatt attattcaag atatgataca cattttttcc
     7261 gcaatgctac ggaaatatat attttgaata gaaaacgctt acaagatatg cttggtttat
     7321 aaaaataaaa ggagtgattg atttatgaaa aaagcattga ttatttgtgc ggcaggaatg
     7381 tcatcctcga tgatggcctc aaagacaacg gatttcttta agcaaaaaag agaagaggta
     7441 ctcgtagatg cagtttcagc tacagaaggc gataatatga ttaagaatag tgattttgat
     7501 ttatttctga tcagcccgca aacgacgatg tttttggata aattcaaaaa attaggagaa
     7561 tctgttggaa agcctgttgt tagtatccct ttccaagcgt atgtcccaat ccctacaggc
     7621 atccagaagt tagcagaggt gatcgaagag aacatatgat ggaataggag tggatgataa
     7681 tgaaacagaa ggaaaaattg ctgttgagat atttgattga tcacaagaag gagtatgtca
     7741 ccagtcaacg tttagcttct gaattgttat tatctgatcg gacaatccgt aattacttgc
     7801 agcggatcaa agagttagta gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg
     7861 gctaccaact gcatatttta caaagactga catttgactt gtttttgagt cggcaagaaa
     7921 tagtacctgg atatgctaga gaaccgcaag gattttatga gtccgaagat cgacaaaagt
     7981 atatattgaa ccaattgctt ttggaagatg ccattctttt tatggatgat ttggcagaag
     8041 agctttatat cagtcgttcc agtttgacaa aggacatgca agaaatcaag gagcgattga
     8101 ttccatattc actaaaaatc gtttctaagc atggtcaagg gacatggatc gaaggggaag
     8161 aaagaaatcg acggcatttt atcatggata ctttttttgg aaaccattat ggaaattcat
     8221 tgaaagaata tttagggcaa agccgttttt tcccagacat cagttttgaa gagctagtca
     8281 ttatcattct cgatgaaaca agagaagcta agttaaaagt ttcggatttt atcatacaga
     8341 atctgacttt acacttggct ttggggatca aacggctccg agaagggttt gaaatcaagg
     8401 aattaggcat cgaacaagaa gtttctaagc gagtagagta tcaagtggcg cagcggatcg
     8461 ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga agaagaaatt gcttatttga
     8521 cattacatct catggctaag tcgaatcaca aagaaaatca aaatgatcag gaactatcaa
     8581 aggaattagc gaaagttctg aaaagattgt ctcaggaatt tcatttttca ttagtagagg
     8641 atctccagct tttcaatgga ctactcgatc atttgtcacc gatgatgatt cgattgagcc
     8701 gaggtattcg cattgagaac ccattgactg aggagataaa gaaagaaaat cccaaagtct
     8761 ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt gaaaaactat accatcaatg
     8821 aagatgaatg ggcctatttg gctttgcatc tcatggcggc attggaaaaa gaaagagctg
     8881 cgcataaact acatgcattg attatctgtg caacgggtta cggcagtgcc caactattaa
     8941 agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt ggtaagtgta aaaggctatt
     9001 atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat tatttcttct attgatcttt
     9061 cgacaatgtt tttcaaaatt ccagttttac atgtcagtat ttttttgaat gaagatgatg
     9121 ttcgtaaaat acgaaaggta gttggagaat ctatacccta tcattcctca gatagaccga
     9181 taccagcatt ttctctacag caaaaaaagc agatttacac cgaacagttg tctgaaaaat
     9241 ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa
     9301 agctgagcat aaatgaagaa gaaggatata taagagaaat gcgaaagcag atccagcaac
     9361 gtgaaaatat gggacaaatc atattcagtg atacagtggc agtcccacat cccgctgttc
     9421 cagttggaat cgaaacaaag gtagccgttg cattgattcc ttctgggatg cagtgggatc
     9481 aatatcaaga aattcatttt gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa
     9541 ttacggtcgt gactaaagca atcgttaggt tagttgacca gctaaaagta cagcaggaaa
     9601 tcttagctga acctattttt gagaatttca gcagtaaatt tatcaaactg atcgaataag
     9661 gaggaaaaca gatggctgaa aaaataaaca gtgaaacact acaagtgact tcttttgata
     9721 tcattttcca tagcggaaat gcacggacac tgatacatga agcatttcgc tcgatgcgta
     9781 aagagatgtt tgaagaagta gaagagaagt tgcgacaagc aaatgaagag attcttcatg
     9841 cgcatcagtc acaaactcaa cttctgaaag aatatgccag cgggacaaaa atagaaatgg
     9901 aaattatcat ggtccatgca caagatcatc tgatgacgac gatgacatta ttggaagttg
     9961 cacaggaaat gaattatttg tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg
    10021 atacagtaaa aaggaaaatc gaattagaaa aaaagcaact tagtttgaaa aacatgtatt
    10081 tcaatcgtta tctaatcgta cgttacctga ctgccttttt ctttttcatg aacatgcaat
    10141 ggatgattct gcttctatca gctaaaagtt taggaagtag tttaccgatg attctgattt
    10201 tagctatttt gcctgcagta ggagaacaag taaagcttta tcgaaaacac caaacgaacg
    10261 ttccatggac aaagtggtat tttttgttcc agagtgtctt taatttatta gtgattccag
    10321 tgttgtttac ctcaggattt acattcttgt atccttttat ggcaaataac aatggaggac
    10381 agttatttgt tttcattctt gttgtaagcg gtatttttgc cagtgcattt atacagtatc
    10441 gtttaaagaa aatttcactc aaccaagacc aacagtatat ccgtattaaa caatatgaaa
    10501 aagcgttata tttaggaaag gagaataact aatgtttgat tttttgcaga aatacctgat
    10561 gggacctatg gggaaaattg cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat
    10621 ggcttctatt cctttcacga ttgtaggctc gatgtttttg gttttaaata ttttgccatt
    10681 accgttccca tttttagaag gattttttaa tgcaactttt ttcaaagtta gtgacttgta
    10741 catgatcgtc aatacaatga cgatggggat tttatctatt tactttgcca ttgtgtttgc
    10801 ttatgaatta acgtcaattg aacgagatga acaaaaatta aatgttaatc cgctcacggg
    10861 ggcattacta tctgtttttg cattctttat gtgtatccct gaattgatca tgactgatgg
    10921 taaaatcagc ttgattgctt ctatcaatga gacagaaact gtagtaaatg gtattaggat
    10981 gggctctttt gtcgaacgtt taggaacttc aggaatcttt acagctatta ttatgtcgat
    11041 tattgcggtc gaattgtatt gtatgtgtgt caagagaaat tgggtcataa aaatgccaga
    11101 tgttgttccg ccaggagttt ctcgttcttt cactgctctg atcccaacat ttgtcattgc
    11161 gtttgtggtg atgatcatca atggattatt agttgcacta ggaacggata tcttcaaaat
    11221 gattgctatc ccatttgggt ttgttacgca gttgacgaat acatggcttg gtgtcatggt
    11281 gatttatttc ttgatccatg cattgtggat cgtagggatc catggagcga atatcatcac
    11341 ttctttcttg accccgatcg tattagctaa tatggcagct aacgcacaag gcgcaaatta
    11401 tccgttggca ggagaattca acaattctta tgtaaccgtt ggtggatcag gtgcgacttt
    11461 aggattgatc atctttattg cattcctcgc gaaatctgat cagctgaaag tgttaggaaa
    11521 agcctcactt gtaccaggat tattcaatat caatgaacca atcattttcg ggatgccaat
    11581 cgtctacaat ccctatttag cggttccgtt tttcttagct ccgatggctt ctgctacact
    11641 cgcttatttt gccattcggc tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc
    11701 ttcaccagtc gggatcggag catttgttgg tagtggtggt gattggaaag ctgctgtgtt
    11761 agcagtcatt tgtgcaatca tcgcattcat tatctggttc ccatttatca aattctatga
    11821 taataaatta ttaaatgaag aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa
    11881 atgatttcta agaaaaaagg aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa
    11941 tgggaaacca ctagtatata aataccatta ttcttcaatc aaccgcttca cgattgcata
    12001 atccccttca taaggttcat ctacaccatt gattttttga tagggatcca cgccttttcc
    12061 cgcaataacg actgaatctt gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg
    12121 acgatccatt tcataatgaa cgatcaaatc tggattagta attgcttcat tgatttcttc
    12181 cgcgattttt ttcggatctt caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc
    12241 tgtttctgat agcacttttc caaaatcatg tcgacgggag attgctttgt ttcctgggct
    12301 gcctaataca acaattacac ggccatctgg atgttcattt ttggcaaatt ccagcaatgt
    12361 tttcaagctt agataattat gtgcatagtc cacatagaca tgcgctccat tggattggat
    12421 caattgatcc attcggcctg gtacacgagc ttcttttaat ccgtcttgtg catcttgact
    12481 ggaggcacct gttagtgctg cggcaatgat cgcactggct gcattttctt ggttgaatcc
    12541 tccagccagc aaaatatcat aagcagtatc gcagatagct aagcgatctt catgagaaga
    12601 taaagtaaaa ccgtgagtac ctttatctga acggatcact tgataattgc tttcttccga
    12661 acgaccataa gtgatcgttg gaatctggaa tagatcggct gtttccctaa gtaaatcaaa
    12721 atagtcactt tcatgattta aaacaactgt ttttgaattc aagatcaatt gtcgcttgca
    12781 atagaaatag tcatcaaatg tcggatgttc gatcggactg atatggtcag gcgagatatt
    12841 caagaagatt ccgacatcta aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga
    12901 agaaacttcc atcactagat gagtcatacc attttcaact gcttctgaca tcatacgata
    12961 taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt
    13021 tgtattcata gtagaaagca atgctgtttt tttatttgtt gtgtgatcca agattgcttt
    13081 tgtaaagtac gcagcagttg ttttaccttt tgttccagtg aatccgatca ctttcaattt
    13141 attttgcgga tagtcgtaaa aagccatagc aagaagcgcc attgctttac ggatatccgt
    13201 tacgatgatt cctactgctg aattttcgta tggttgttct gatacataat attgaatacc
    13261 ctgttcgatt gctgaatcca aatattcttc tttgaaattc attcctttac agaaaaacaa
    13321 agtagaagca tcagcttttc ttgaatcata acttaactga agaaattcag ctttttctgg
    13381 tacatcaaga tgccagcctc ttgttgaaac aaattctttt aatagatttt cttttaatag
    13441 taaatgacgg atgtcttcta aagagaagct cattgattga tccctcgctt tattcaattt
    13501 cttggtttat tatagcacac tctttttaat atatcgtttg tttccgctat gacaaaatcc
    13561 agctttattc aaattttctt gacacagggg acaagcggat gagtattttc ataaacaatg
    13621 aactgactgt aatagatagg agaacgaata gtgtctccaa caatccaaaa acataataat
    13681 tcaaaaagac gataagacta ttggtaagta aaagtccttg cggcattgga agccgaaaaa
    13741 atttgtggaa aataatcggc ggtatcgtta taccgccatt tgaggccccg actgtgtaca
    13801 atgtgtaaac cccaagactg aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg
    13861 agatcatttc cacttttgga acaatctcta aaacaacagg aaaaagcaga cttcccgcta
    13921 cagtcttaaa aaatactgat tttcctaata ggacagcggc tagaagcagc atcataagat
    13981 tgatgctcca aacagtaact gagcgttgga tagaaaaggc cgtttcaact aaatgtccga
    14041 tggcgcccac tccggcggaa gctatatcgt gagggagcaa gaaccaattc aatccaactg
    14101 tgataaagaa aagacctaaa aatataacgg gtagtttttt tatttcttgt gtcattgata
    14161 tctcctataa atgttagcca taaaaggctg tatatttctt tactttacca aaaaaacgaa
    14221 accgaattca aaaaaacaat tgttcttata aaagttatat aaaatcgatt gccagtttct
    14281 gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt tggaatctga ccgatggata
    14341 atcaaagaaa accagcatta gatcaattaa caccgcaaga aaaaatggca cgaggctctg
    14401 cttggatgac cgccagtaat atcatttcaa gattgctagg cgctgtctat atcattccct
    14461 ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg attattcaat atgggataca
    14521 atatatatgc attgtttcta ctgatctcta cagcaggaat acctgctgcg attgcaaaac
    14581 aaactgcacg ctataattca ttgaatgaat atggcacttc acgacgatta tttatccgtg
    14641 cattacagat gatgggcggg ctaggcctgt tgtttgcttt gttcatgtac atcgcctctc
    14701 cttggctagc tcatgcttct ggtggcggag aagaactgat tccaacgatg cgttctttga
    14761 gtatcgctgt attggttttt ccttgtatga gtgtgatacg agggtatttt caaggcaatc
    14821 aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca ggtggcacgt attttttata
    14881 tgctattggc tacattcatc atcatgaaag tcatggatgg tgactatgtg acggcggtca
    14941 cgcaatcaac tttcgcggca tttatcggta tgctagcaag tattcttgtc cttttgtatt
    15001 ttttgaagaa acatcaagcg tatacatcgg cgtttattca ctacagcgaa aacaaaataa
    15061 atattgcaac gaaggaattg ttgttagata ccgttaaaga agcgattccg tttattattg
    15121 tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt cacttttatg aagatcatgt
    15181 ctgcatccac cgagtattcc aatgcacagt tactggatct tttttcgatt tttagtgcca
    15241 atccagataa gttgacgatg gtagttattg cattggcaac atcgatcgcg tctactgggt
    15301 taccgttgat tacagaagct gttacgttga aagatcgagt aggtctggca aaattgacaa
    15361 gcggaaattt acagttgttt tcatttttta tgtttccggc gacatttggt gttatgttac
    15421 ttgcctatcc gctaaataca ttattttata caccggattc tctaggaagt aatgtactga
    15481 tccaagcaag ttttgtagga ctcttcctag gactatatat gctagtttcc aacatgctgc
    15541 aaggaatgtt tgaaaacaag gcggcaatta attatttggt tattgggttt ctagtgaaac
    15601 tccttcttca atacccagcc attcgtttat ttgaagtata cggcccttta ttggcgacaa
    15661 tgatcggatt tgctgtgtct tgtaccttga tcttaaagag aatccatgaa gtagcccgct
    15721 ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt tattcttacc ttgattatgc
    15781 ttttggcttc gtggatcaca aagatgattt ttggtgcttt tttaaacgaa gatagcaaat
    15841 tccagtctct attgttgatt cttgtagtag caggtatagg ggctttagtg tatggttatt
    15901 tagctttgaa aatccgcttg gcagaaagac tactaggacc aggaatagcg cgtttacgac
    15961 ataaattaaa aataaaataa gtgaatcaga agagctagat tgatgctctt ttgaaatggg
    16021 ggaaacgcat gcgtttagat aagtttttag cagatatggg acttggcagc cgcaaagaag
    16081 tcaaaggatt gatcaaaaaa ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata
    16141 aagcgcaagt aaatgaattc gaagatgcgg ttacttattt aggagaaatc attacgtatc
    16201 aaaaagattt ttactacatt ttgcataaac ctgcaggagt catttctgct actcaggata
    16261 atcatgatca aaccgtaatc gatttattgg aagaccagga ttatcgggaa gatctttttc
    16321 cagtcggccg attagataaa gacacagagg gcctcttgat tttaacgaat gacggggtgt
    16381 ttgcccatca gttactatca cctaaaaaac atgtggagaa agaatatcta gctgaggtta
    16441 gcggtataat gacagaagaa gatatccgtt tatttgcaga aggactgatt attgatggcg
    16501 atgagcagac attgccggca aaactccaaa ttgatcaagt agatcaagag acagaaactt
    16561 caattatccg tctgattttg catgaaggaa agtttcacca agttaagcga atggtcaaag
    16621 cagtaggaaa agaagtctta tatttaaaaa gaatccgtat gggaaaattt tggctgccta
    16681 aagatttgaa taaagggcaa tatcgaccca tgaccaaaga agagctagaa caagtgagag
    16741 aaaaataatt gaatggatag ctaaaagaat aagcagagta gttgtagcgc catctgaaaa
    16801 gcagacttgt ccgtctgact ttcggatggc ttttttgtga aatttcggct tatttccgta
    16861 gaagctactt ctggaacacc gtttgttcgg aataagggac tgtgacaaga cttttgttac
    16921 agtccccatt gttccttgtg actcagagtt agatagtaag gacacgatct ccctaatact
    16981 caggccaaaa cgctgaattc acaacctctg ataaacggtg ttcaatcagc tgaccctcgg
    17041 tattagctca aaaaacggca aaatatgagg aactattttc ccgttttttt ctctaatact
    17101 caggccaaaa cgctgatttc acaacctctc ctatactttg tttcattttc cctatacttt
    17161 gtttatttat tctcattatt gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt
    17221 ttttagatga aaaaacgaaa ggggttcaat cattttttta tgaatctatg gcgttataaa
    17281 gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt ttacatacct
    17341 gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg gttcttggct
    17401 agcttgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt ttcttcggat
    17461 caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat ccttttgaat
    17521 ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa acgactcgtt
    17581 aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat tatcagttac
    17641 tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat cactggacat
    17701 ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat cttggtgttc
    17761 attggtacat ggaaagggat tggttataac agcattattt actttgcatc tgttatggga
    17821 atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg gcagcagatc
    17881 aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat tttagccgta
    17941 ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa ttccggtgca
    18001 ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac atccacagga
    18061 gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt tgtcttgttg
    18121 atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt ctaaggagga
    18181 aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat aagacggcta
    18241 attttttctc taatataatg attgcactct ttgctatttc ctgtattttg ccatttatat
    18301 ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg tacagttttt
    18361 ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg caggataaaa
    18421 ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg atcaatagca
    18481 cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc cgtcgttttt
    18541 ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc aattatcttg
    18601 ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg ccaatggctg
    18661 tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc tctgattcga
    18721 ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt cagatcgttt
    18781 taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg ggttactgga
    18841 atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca ctacagtatc
    18901 ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga gcaggaagcc
    18961 aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg atcgtagtca
    19021 tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt gtcaaaggtc
    19081 taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa attaggagga
    19141 agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt gttgttggga
    19201 acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag caattcggac
    19261 actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt gatggaagta
    19321 gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta cattggctgg
    19381 ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta tgatatcgcc
    19441 tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt gactgaatta
    19501 gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat taaaggaaat
    19561 ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc tcaacaagta
    19621 cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg aatccaatct
    19681 tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct
    19741 tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt agggaatacg
    19801 ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca atacgataat
    19861 gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg actcattcca
    19921 agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg gctaatgcgt
    19981 ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc agcaaaacaa
    20041 gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc acaaatggca
    20101 aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact tctagggctg
    20161 cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg agaagcatgg
    20221 gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca gccaaataca
    20281 catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga gtctattacg
    20341 gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc accgatttta
    20401 ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc taatgtcatg
    20461 aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac attaccgaaa
    20521 ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat gcaaaaacaa
    20581 tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg acaaaagacg
    20641 tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg gtattaagct
    20701 aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta ctcgaaacta
    20761 agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct cggtattcgc
    20821 tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat aatcaggtca
    20881 aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta atgtttggag
    20941 gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg gtcattataa
    21001 aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt ggattaggtg
    21061 cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat aaagaaatca
    21121 caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat ctgctattta
    21181 gtttattttt atctggtact gctttattga gttacaattt atatttatct gttcaaatca
    21241 aagggctcat ttttttagct attgatttta ttttactttt tgccttgctc tgcctatttg
    21301 ctacttatca atacagtatg attttagata gtgaatatac gatctctgtt cctaatctgc
    21361 taaagctaag ttttatttcg gttttttcca gcttttcgtc ttttctaaaa ataattattg
    21421 gtagcggtat aattttaggc gtgacttggc aattcaaagg attgatcctg tttggtgtca
    21481 ttggtctttt gacggtttgg aatggtacga tgacaacaca ttggcgtgaa gaattagata
    21541 aacagttgga aagttatgaa taagtttcgg attctatttt caaaaggaac cttactaaat
    21601 cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa cgatgactgc taccgttatt
    21661 tttagtatcc atacttggga acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc
    21721 gttcaaagca caagcagaat gctgaatgat aaaacaacct tgagtaggat catcaaaaac
    21781 cagcttgtag ggaattccga aaagatcgaa aatgtgacga cctacttaac aaaacccata
    21841 gatcagtatc tgatgtatgt atacgaacag caaaacagta ccgatgaatt agtttctttc
    21901 ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac taagtgcgat atatatcgtg
    21961 ttgaatcaac taccggagta ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa
    22021 aaggggacac caaaattact agacgccttt tatatcaagt tgcctatcac acaaggagga
    22081 gctgagtcag gaagtatttt tattggtttt caaaagaaag aactagatat gattttagaa
    22141 aatctaacgt cattcaatgg actctcttta tacatgattt ctggaacgac aaatcgacta
    22201 tacacattcc atgataataa aatagctaaa gaaacgtttg aaagacaaga aatgatcatc
    22261 acagagagtc tgaagaaaaa ttcacaattg ccgattgacg atctggaaaa aaacaatttt
    22321 gttcaacacc aagaactgtc tgatgattat cgtatattag ctgtactaga taaaaaaagt
    22381 gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca ttggtatcat tattttagac
    22441 aatatactgc ttatctttct ttataaaaca ttcaaacgat attcacagca agtatctatc
    22501 gtcatgcagg ccatggatca aacagcagca gggaatttag agacgcggat cgacataaca
    22561 aacacggaat atgaactgaa ggaactttca atcggtatca acgaaatgtt ggatagcatc
    22621 aatcagtttg tagaagacat ctacaaatta gaaatcaaac agcaagacgc acatatgcgg
    22681 gcgcttcaag cacagatcaa tccacacttt ttatataata cattagaata tatccgaatg
    22741 tatgcaatca gcgaaggaag tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg
    22801 cttcgaaaca atacgaatca agagaaaacg atcactttaa aagaagaact ggacttttgc
    22861 gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc gagtagccta tcattttatg
    22921 atcgatcctg atttggaaaa gatcgaagta cctaaatttg tgatccagcc attagttgaa
    22981 aattatttta aacacggtat tgattttact cgattcgata atgcgctaag tgtcaaagtc
    23041 ttgcaggaag gcaaacgggt aaggatcatc atcaaagata atggaaaagg gatgactgaa
    23101 aaacggctaa aacagattga agaaaaactg tctcatccta aagtggaact tcacggttct
    23161 atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct tcggcagcag ttattatatg
    23221 tcgcttgaga ataatgagac aggtggactg accgtttcta ttacattcaa ggagggatga
    23281 gcgtgtatca agtgttattg gtagatgatg aatacatgat tttgaatggg ttaaaaaaga
    23341 taattgattg gcaaagttta ggctttcaaa tagtagcaac agcagagaat gccaaagaag
    23401 gactagctgt acttgaacag cgacagattg atttagtagt aacggatgtg acaatgccag
    23461 aaataaatgg actggaattt attgaagcgg ctcaaaaaga acgtcataat tttgaattta
    23521 tgattttatc aggataccaa gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg
    23581 tgaattattt gatgaaaccc gtgaataagt tcgaattggt tgaaagcctc aagaaaatca
    23641 aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca agaaatctat caagaactgt
    23701 tattcagtca atggttgaat gaagaattgg atgaaataag tgaagaagaa ataataggac
    23761 aaataagtga gaagcagcat aggatagtat tgattcaact ggcaagaact catgatactc
    23821 ttatcaatag atggttgcaa gaacggcaac aaatctttta ctatcaacga aattacggag
    23881 accgtgtatt gatcacgttg ttgttggagg cagatgaagt tgatggcttc tgttgttttg
    23941 ttcaagaaaa ctttcctgat caggaatggc tgatcagtat cggggaagag acagattctt
    24001 tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa tctacagttg catcaatttt
    24061 atggagataa agacgagcat gttttttacg cagtacaagc agaaacaaaa gatcaaacca
    24121 tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa
    24181 tgatcactga tttttttgaa cagtttcagt tagcagccat gatgccggaa gatatacgat
    24241 attccagttt tttactgttt atggagattc aacgggaatt gattgatctg gaagatgagg
    24301 aatatttaca aggaatcgaa aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc
    24361 tacttctgtc attcatcaaa aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa
    24421 aagtaatcgt aatcctgcac cagcattatc aagaaccact tacactcaaa gaagtgtcag
    24481 aaaacctgca tttaaatgtc atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa
    24541 gtttttcagc ttatttgaac catttacgta tggaaaaagc gaaacaactg cttttgcaca
    24601 gtaatcaaaa catcaatgag atagcaagtg agattggcta caacaacacc acgtatttct
    24661 ctaaattatt caagaaaatc gtcggcagat cccctaaaga gtaccgagaa aacagtggag
    24721 aatcataaaa aaaaaagcca atgtaatgaa ataacttata aaaaattgtc tagaaatcta
    24781 attttgggct gttcttatta cttgaggcaa ggcagctttt tcatgacctc tcgataaacg
    24841 gagttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat
    24901 tttcacaaaa tttttcttaa tactcgaaac taaacagctt tttcacgacc tctccactaa
    24961 atggtgttca atcagctgac cctcggtatt agctcaaaaa atggcaaaat atgaggaact
    25021 attttgccat ttgtctctct aatactcagg tcagaacgct gatttcacaa cctctcaata
    25081 aacggtgttc aaaagcttgc atctgcggta ataagaccaa ccaaacgaaa aatatgagga
    25141 tctatttttg cttggctgtt cttattactt gatgcaggac agctttttca caacctcttg
    25201 ttaattatag tttgataagg aaaaaggcaa atcttttgct tgatatttct tagggtgagg
    25261 gatgatccct aattggtaag agatcgtgcc gcctttcagc aaatcttgat gggtaaaata
    25321 taatttcgta tattctctgt ccatccgttt gattttatgg ataaattgtt gctgcggccg
    25381 attaggcgtt gcttcaatcg tcaattcctt tccatttgac aaaagcaaaa cagccttgtc
    25441 aattaatggc ataccgataa catattcacc ggttccaggc gtgacaggat aaaatcccag
    25501 actattgaag atataccaag cggccatact tccattgtcc tcatctccag ggtaacccat
    25561 tggagaatcg tcgaaggtct gagtcatcaa ctgtttaagc agtggctgtg ccatttcagg
    25621 ttgtcctata taactgaaca agtaaggata gtgaaaactt ggctggtttg aaatagctaa
    25681 ttgtccaaat tctatcgcgg ccatctcact catttcgtgg atctcaaaac catatcctcc
    25741 tgggtcaaac gttgggggct gattgcacag ctcaatgagt ttttctttat atctctcact
    25801 gccaccatat tttccgatca gtcctgaaaa atcgtgataa acagcaaagc tgctttgcca
    25861 agcacttcct tctgcataat cttgtcccca acgattagct gaaaatggtt ctctgaactt
    25921 gccatcttta tcttttgaac gcataaatcc tgactcatag tcaaagatat tttgatagtt
    25981 caatgattgc tgctgatatt tcttcgtcat ttcctcttct tctagtacct tggcaacttg
    26041 gctaatacaa aaatcgctat aagcatagtc taatgtgtga ttgactgatt cgtggtagtg
    26101 attaggaaca tagccatatg tcaagtaatc ttctgttcct tggcgtccat aatttggatg
    26161 tttgctttga accgtagcac ctttttccat tgccgttaga aattcaggca tcaagtctcg
    26221 tcggatatct tttacagcag catctgcaat gaccgcatcg atcaatgtcc caggcataag
    26281 tccacgttca tcaggagaca gccattttgg caaatagcca ctttctctat agctgttcaa
    26341 aaaaccttcc agcatttctt cgtatttttc tacagcaatc aaagaataaa gaggataaac
    26401 cgttttatac gtatcccaaa acccattatt tgtatagaga acaccaggtt ttatagatcg
    26461 actagtcgta tcatagtgga ttttttgccc actaggatca atttcataaa atgtttgtgg
    26521 aaataaaaac acccgatata aattatgata aaacgtccgc aattgttgtt gatcatggtg
    26581 tgtgagctgt atacgatcaa aataggactg ccattgttct tgactgtgag caaggtaagt
    26641 ctctttgctt tggttttgtt ctagttccag gttataagct gcttgttccg agctgataaa
    26701 agatgtccca aatcggatag tttgtttttt tgtctgttca aaatacaacg ggatcgagtc
    26761 gctatttttg ttggaaacag ctaactcatc aattgttaaa ggatattcaa atctcaatat
    26821 aaaataaaaa gaaaaatcag gatcttctga accagcaaaa tgtgtgattt ttccttccac
    26881 tgtgtaagga tctataacaa agagctgatg gcgtcccgga aaggataaaa gtaaaccgct
    26941 gtcatttttg ctgtaatcaa tagataagat tccgccatac atgctaggta tcaatgtaga
    27001 acagatgcca taacgtaatt gacagatcga cagatgagtc gggcaaaata tactttcttc
    27061 tgggcggtaa gaactttgag catggaaaag ggtgttctcc tgtagttttc cattgatcgg
    27121 cgtaaacagc atatgactga aatcacccat ccaaggactt ggttgatggg taagacggta
    27181 gccttgaaaa acgcggtcat caggatgaaa ccaccagctg ccgttttgat ccgtagtttg
    27241 cggtgcaaaa taattcatcc caaaaggaac gccagtataa ggctggcagt tcccattaga
    27301 gaaatttggc tgattcgctg ttgcatgtct agtatctatt tgaccgatat tcattttatt
    27361 tctcctttgt tgattgtctt ttgatgaatg aaactggaaa tttactatcg gatggtcgat
    27421 cttttgtctc taaccaagtg accaaagcat ctcccgcaag cttacctaac tgttcaaaat
    27481 cttgtgcgat ggttgtcagt ggcgggtcaa caagagctgc tgcttggata tcatcaaatc
    27541 cgataattga aaaatcttca gggatatgat aaccatgctg tctcaagaga ttcatggtct
    27601 ggatagcgac aacatcgttt tcacaaacaa aagcagttac ttcatactta gtgatataag
    27661 aaagcaattc atctaatgtc gcgcttttgt catccagtga agtgtggaaa gtcaaaccag
    27721 ctttgtccaa tgcttcaagg taaccaaggt aacgctgtcg aacggattgt ggatgagtct
    27781 gcgtaccaaa caaataagcg atttttgaat gtccctgttc gatcaaataa gaggcagcaa
    27841 gcgcaccgcc ttggaaatta tcggaaagaa cggtaggaaa aggtaactca tagatttttt
    27901 tatctaatat gacgacaggg aattctttca atgaaagctc aaaaagtaag tcaagatgtt
    27961 gttctgtatc aagagcataa taaatcaatc cgtcaaattc attgattagc tcttttgctg
    28021 ttttattttg tagaaagtca agcgtcgtca tcatgacatc aatctgttta tcttgcatca
    28081 cagggttcaa tcccttgcta aaatcaccaa cagacaaatc agcgacatat ggaagaagaa
    28141 acagaatccg tcgtgttttt gtaggagaag caagaatctg ttccggagaa gcggatttaa
    28201 cgaaactgcc tttcccgcga acgcgataaa taagtcctaa ttgctctaat tcagtcaatg
    28261 cacgtttgga agtgattctg cttactttat aggtacgtga aagttctttt tcagttggta
    28321 gttggctgtc tacaggtaat tcaccagtta aaatagccgt ttgcaaatca ttaaatattg
    28381 tctggtacaa tggttgagtc atataaacac ctcttaatga tatatcatat tcaaataata
    28441 ccataatgga tgaaaaagta aataaaaaga tattttttga aagcgtagac ataattggct
    28501 gtatgaagta taatctttaa agatatatca aatagaaaga aggttttatt agtggtttat
    28561 aaagatatac caaaatcggt gcagaaattt atggaagata tcacacaaaa atgtggggat
    28621 atccatgcgg actgggcaaa gaattttaca gcagcatttg ccaatactct gttgacaaca
    28681 gtcaaaaagc aaaatgacgg aaccaccttt ttattgacgg gagacattcc agcgatgtgg
    28741 ttaagagact ctactgcaca agttcgtcct tatttagtga ttgcaaaaga agatgaggaa
    28801 ttagctgaca tgatcagcgg gttagtcaaa aagcaatttt tttatatcaa tatcgatcca
    28861 tatgcgaatg ccttcaacga agaagctaac ggtgctggcc accaaacaga tcatacaaaa
    28921 atgaacgatt ggatctggga aagaaaatat gaaattgatt ctttatgcta cccaatacag
    28981 ttggcctatc ttctttacaa aaatacagga aaaactgatc aattcaatca atcttttatt
    29041 gaaggtatca aaaaaatctt ggacgtgttc aaaacagagc aagatcatga acactctcct
    29101 tatacatttg tacgagacac gacaagaaaa gaagatacgc tgatcaatga cgggcgaggt
    29161 gcaaaaacag cttttacagg aatgacatgg tctggtttcc gtcctagcga tgatgcttgc
    29221 cgttatggat atctggtacc atccaatatg tttgcggtgg tcgtgttagg ttatgtggaa
    29281 gaaatttttc aaacgatact cgatgataaa gaaattgtac agcaaacaca gaaattgaag
    29341 aaagagatcc aagaggggat tcagaatcac ggacgaacgg ttaatcaaaa agggcaaacg
    29401 atttatgcat atgaagtaga tggaatggga aacgccacag tcatggatga cagtaatgtg
    29461 cccaatctga tcgccgcacc ttacttggga tattgttctg aacaagagga agattatctg
    29521 caaacaagaa gaacattgtt gagtaaagaa aatccatatt tctatgaagg aaagtacgca
    29581 aaaggaattg gatcctccca tacgccagaa aaatacgttt ggccgattgc attagctatg
    29641 gaaggaatga caacgtcaga caaatctgaa aaagagcgga tattgaattt acttgtagca
    29701 actgacgcag gaacacacct tatgcacgaa ggattcgatg tgaacaatcc tgaaaattat
    29761 acgagagaat ggttttcttg ggcaaatatg atgttttgcg aattagtcat ggattacttt
    29821 gatattagag tagaaaagta ggaggtatcg taaatgaaaa agaaagtata tatcatttct
    29881 catagccact gggatcgtga atggtacatg gcttacgaac agcatcacat gcgactgatt
    29941 gaattgatag acgatctact agagctattt gaagttgatc cgtccttcaa cagttttcat
    30001 ttggatgggc aaacgattat tttagatgac tatcttcaag taaggccaga aaaaagacag
    30061 gcaatccaac aagcaatcaa tgaaggaaaa ttgagaattg gaccatttta tatcttgcaa
    30121 gatgattttc ttatcagctc ggaatcgaac gtgagaaata tgctgattgg catggaagaa
    30181 agccgtaaat ggggaacccc tgtcatgtta ggctattttc ccgatacatt tggaaatatg
    30241 ggacagacgc ctcaattaat gaaacaagct ggtatttcag cagcagcgtt tggccgtggt
    30301 gtaaaaccaa ttggctttga taatcaagtt ttggaagcag aaaactattc ttcccaatac
    30361 tcagagatgt ggtggaaagg ccctgatcaa acagcaatct ttggtctgct atttgccaat
    30421 tggtatagca acgggaatga gattccagta gaaaaagaag cagctcttgc cttttggaag
    30481 caaaaattag cggatgcaga acaatatgcc tctacgaacc acttattgat gatgaatggt
    30541 gttgatcatc aaccggtaca aaaagatata tcaaaagcaa tccatctcgc aaatgaactg
    30601 tttccagatt acgagtttat tcattctaat tttactgact atctggaagc agttcaaaaa
    30661 gatgtgccag aagacttagg ctcagtagaa ggagaattga caagtcaaga aacggatggc
    30721 tggtatactt tagccaatac agcatctgct cgggtttact taaaacaatg gaataccaag
    30781 gtccagcgac agctagaaaa tattacagaa ccacttgcga cgatggctta tgaagttagt
    30841 ggaaactatc cccatgatca attagattat gcttggaaga cattaatgca aaatcatcct
    30901 cacgacagta tttgcggttg ttcagtcgat tctgtccatc gggaaatgat acctcgtttt
    30961 gaaaaagcag atgaagttgg aaagtatctt gcacaagatt ccttagaaca gctgaccgca
    31021 gcaatcgata cgaccggatt cccaaaagac agctttccat ttgtcattgt aaatactgca
    31081 ggaatggata aaactggtga agcagaaata accatcgaat tagaaagaaa aagattcgct
    31141 gaaggaatac ctgaacaact atatcaagag ttagaaaatt tgcctaaacg taagtatcat
    31201 gtagaaacaa aaagtggggc cactatacca gctattttat ctgaagaaac tgtccaattt
    31261 ggttatgatt tgccgaaaga ccgtttccgt gtgccatata tggcaagaat gatcaaagtg
    31321 actttaccat tagaaaacat gccagcattt tcttgggaaa cattcgcgct tgtagaaggg
    31381 gaagcgaaag cagaagaaaa ggaaacaatg attcatcaga gtggacgaat catcgaaaac
    31441 ggccctctac atttgacgat tgaaaaaaat ggtaccatta caatggaaga tcgcaaaaac
    31501 aaacgaaagt tgaataatct gcatattttt gaagatatcg gcgatatagg aaatgagtat
    31561 atctttaaac aacctttctg tgatcagcca atcctatcca gtaacaaaga aaactctgaa
    31621 gtaaaagtat tagtagatac accggaaata gcaaaaataa gtattctaca ggaaatggaa
    31681 atccctgttt ctgcagacga gcgtttagaa aaagaacagc agatggttgt ggaatttcgt
    31741 tatcgaaaag cagaacgatc caaagaaaaa cgaatccttc aaataaaaac aatcatgaca
    31801 atcagaaaag atagtaaaaa aatcgacttt gaaacgactc tcgacaatca gatgaaagat
    31861 catcgcttac gtgtcttgtt tcctacaaag ttacatgtgg aacatcacga agcagatagt
    31921 atttttgaag tggtgaagcg cccaaaccat gtgtctaaaa gttgggaaaa ccccacgaat
    31981 ccgcaacatc agcaagcatt tgtcaatatt catgatgaag agtacggtgt aacagtcgga
    32041 aactttggtt tgaatgaata tgaagtaaca gaagacggac aaatcgcagt gacgctactt
    32101 cgcagtgtgg gagaactagg cgattgggga tattttccaa caccagaagc acagtgtcta
    32161 ggtgagcatc ggttcaatta cagcattgaa ctacatggac cagaagaaaa attctctact
    32221 tatttgcatg catacgcagc acagattcct ttttccactc aacaaatcaa acatcatgaa
    32281 ggaacattga tttcaaaaca acaatattta acgataaaaa gtgaaacgtt tgcaattaca
    32341 gctttgaaac gcagcaagtt tagtgataaa gtagtagtaa gaggtttcaa tatgtctagt
    32401 catttagaaa aattggaaat aactaaagac aatggaaaaa ctgtcatatt gaatctgctt
    32461 gaagaaccaa cgaagcaagc ggttgtccca ataatccaac cttatgagat tcggacaatc
    32521 ggattcgagg aggaaaacga atgtatggcg gaatagaagc aggcggaaca aaatttgtgt
    32581 gtgcggtttc agatggtgga gaaattttag aaaaattaag tatccctacc acaacaccag
    32641 aagaaacatt ggatcaggta tttgaatttt ttgatcgtta tgaattgaag ggaatcggga
    32701 ttggctcatt cggcccgatt ggtatcgaca aaacaaaaga taattacgga tacgttttgg
    32761 caactcccaa aaaagggtgg gcacaattcg attttcttgg cacaatcaag gaacgctaca
    32821 atattcctgc tgcttggaca acggatgtca atgctgctgc atatggggaa ttgaagcaag
    32881 gagcagcaca aggaaaaaat agttgtattt accttaccgt gggaacagga atcggtgcag
    32941 gtgtcgtgat caatgaagaa attttttcag ggatcgcaca tcctgaaatg ggacatattt
    33001 gggtgaaacg ccatccagat gaccattatg aaggtacttg tccttatcat aaagattgtt
    33061 tagaaggatt agcagcagga ccgtctattg aggcacgcac agggatcaaa gggcaagatc
    33121 tgccgcaaga tcatcctgtt tgggctattc aggcttatta tattgctcaa gcactgatca
    33181 actatacgtt gacgttagca cctgaaaaga ttattttagg cggtggggta atgaatcagg
    33241 atcatctcct tcaaaaaatc cgtcagcaat ttgttgaatt gatgggtggt tatatggaaa
    33301 cacctcaagt aagtgaatat atcgttcgtt ggggactgcc gaatgaaagt gggattatcg
    33361 gtagtttgct gttagcagaa cacgaacatc aaaagtaagc agacaaatta cacaaaaaga
    33421 acagagtctt atcattatct gctgataaga ctctgttctt ttttatttct attcccataa
    33481 tccaggtaaa gctttaccga ctattttttc ttgagtaaat ggatggatca tgtgcagttc
    33541 taatgcatgg agcatcagtc gttcagctgt tctattttgg tagagagggt ctccaacgat
    33601 cggatgtccc ttatgagcta aatgaactct gatctggtgt gtacgtccgg tttccaaaac
    33661 acaatagact tgtgtttgtt cagaaagtac tttgttgaca gtgacatgtg tcaaggcgct
    33721 ctgaccattt ttatcatcta cgaccctttt tctccgatca tggcgatctc gtccaatttt
    33781 attggtgatg gtcatatctt tggcaatttc tccccatacg atggcttgat atctccgata
    33841 gatcattttt tgttccaaca ttcttccaag tatcggcaag acaaatggat tttttgcaaa
    33901 taaaacggca ccgcttgttt ctttgtccaa gcggtggaca acgtaaggtc gttgctgttt
    33961 agtgtcgaga taagcagcta aatgattgag taaggtactg ttttctgtcg gttcatttgg
    34021 atgagtcttc attcctgcag gcttatccac tataatcaaa tgctcatctt catataaaac
    34081 acggatgtgc ttcgcatctc ctaattgaac ttcttgataa ttataatctg tttcttcgaa
    34141 taacaatgtg atctggtcat tgccctttac ttcatgatgg aacaaagcag gttgttgatt
    34201 gatccaaacg tttttacgta tacgcaaaaa gtgtcgaacc tttctaggaa ctaaccattc
    34261 cttttcaagc aagtcgcgaa ttgttgtttc aggatggttc ataggtaaag tgatcgtgat
    34321 tttcacaagt gtactccttt cagtgcaatc gcagactaat tgtaccattt ttaaaacata
    34381 tttaagaaaa aacttacatt tttctgttca ttcttatgtt actctacttg cactaacgta
    34441 aaaaaacata ttgaactaca acttaagcag gaagaaaaca ctctttcact atggtaaaat
    34501 aagacgattg aatcaaatag acaggagaag aagcaaatgg attttcaaag cattttagaa
    34561 aagataaaag ctgctttgat cagtttttgg cgttggatca gaccatatct cggacaattc
    34621 catcgatggc gaaagcgcat ctggaaaaaa tatcatgtga acaaattgat tttgctgatt
    34681 ggtttgatct gtgtcttagt gacaagtatc tacctcttta ttttagcaaa acaagcaaat
    34741 gtcgaaacgt taaaatcagg attaagccaa tcgacagtca tttatgataa gaaaaacgaa
    34801 gaagctggca cactgtacgc ccaaaaagga acatatgtag aattaaacgc gatttctccc
    34861 tatatccaag atgcagtaat ctcgacagaa gataggaatt tttacgagca tcacgggttt
    34921 gatatcaaag ggattgcccg tgcagcagtc cgaatggtct tgtctggcgg aacatcaggg
    34981 ggcggaggaa gtacgatcac tcagcagctt gccaaaaatg cgtatctgac attagaccaa
    35041 acgtttgatc gaaaagcgaa ggaattattt ttggcaatcg agatcgaaaa gaagtacagc
    35101 aaagaagaaa tcttgaccat gtatcttaac aatgcctatt ttggcaacgg ggtatggggt
    35161 gtccaagacg cttccagaaa gtattttgga gtagatgcaa cgaatgttac gctgtctgag
    35221 gctgctactt tggcaggaat gctgaaaggt cctggtattt ataatccgat cgatcaccca
    35281 aaaaatgcga atgatcggaa aaacacagta ctaagtgtaa tggaagaaaa cggaaaaatc
    35341 acaaaagaac aagaacaagc agaaagccag acagatatca gcgcgtatct taatgacaca
    35401 tacgataatt cagattctgg ttatcgttat ccttattatt ttgatgcagt gatcgatgaa
    35461 gcagttaacg agtatggtct tgatgaagaa gatgtcatga ataaaggcta taaaatctat
    35521 acagctttag atcaggatta tcaaaagcaa ttagaagcta cttatcaaaa cagtgcatta
    35581 ttcccagcaa atgcttcaga tggtgccatg gtccaatctg cttcagtagc tttagatccg
    35641 aatacaggtg gtgtacaagc actagtagga cgaagaggag atcatgtctt tagaggattc
    35701 agctttgcta cacagatgcg tcgttcacca ggatcaacga tcaaaccatt aagcgtatat
    35761 acaccagcac tggaagctgg atacaagcct tccagtatct tgaaagacga accgcaaagt
    35821 tattacgatg cgcataattt tgacgggaca taccaaggag aagtaccgat gtatcaagct
    35881 gttgcacaaa gtttgaattt gcctactgta tggcttttgc atgagatcgg tttacaaaaa
    35941 ggatatgata aagcagaaga attcggcctg cctttagcaa aatctgataa gtattatggc
    36001 ttagcccttg gtggactgga aaaaggagta tctccattga tcatggcggg tgcttacagt
    36061 gcatttgcca atgatggtca gatgtatacg ccgcatctta tcacgaaaat cgttgattcg
    36121 actggagcgg tgatcgtcga taacacaaac aaaaaaccaa aacaggtcat atcaaaggaa
    36181 acagccgatg aaatgaccag catgcttctt ggtacatttt caaatggaac agcggctgct
    36241 gcgaatcctg caggttatac gatagcaggg aaaacaggaa ccacagaaac taatttcgat
    36301 gccacaaaag caaatgacca atggatgatc ggttacacgc cagacgtagt gatttcgaca
    36361 tggatgggtt acgaagaatc cagcaagctg cactatttgg aagggacaag tggaaatgtt
    36421 gtcgggaaag tcttcaaatc agcggcagaa ggcatccttc cgtatacttc aaaaacagga
    36481 tttagtgtag cagatgccta tgccacagga ggacaagtgg tagctgcaga tcaagttgga
    36541 aacacaggaa attctaatgg agagtctgga aattggcaag ataacttgaa tcaatatggt
    36601 gaacaagcaa aagaagggct gaaaaacttc ggcgatatgg tgaaagaagg cgttcaggga
    36661 attggtgaag cggctaaaga cctctggcgg aaatatcaag gagagtagcc cctttgaaaa
    36721 cgaataaccc catgttacaa tagagagtaa ctactaaaag aaaagagggc ataacatgtc
    36781 aaacatctac gatagcgcga atcaaatcga acgtgagatc cgtgaattac cagaattcaa
    36841 agcgttgaaa gaagcgtacg agaaagtaca agcaaacgaa gaagcacaca aactgttcaa
    36901 agacttccaa gcgatgcaaa tcgaattaca agaaaaacaa atgagtggac aagagttcag
    36961 cgacgaagat gcaatgaaag cacaagaaat ggctgtgaaa atccaatcag aagaagtaat
    37021 caatgacttg atgcaaaaag agcaaggctt cagtacaatc atcaatgact tgaaccgtat
    37081 catcatgaca ccagttcgcg acctttacag cgaataaaac tattttatga aacaacagag
    37141 ggtgtgaaag agacgttcgg ccttgagcga tgacaaaatt gcttcttatt gccgataggc
    37201 tgtctcttga acaccattta ccaagaggtc gtgagcctac cgtttaactc tgagttacaa
    37261 ggaacaattt caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga
    37321 gttggcagga gaacaccgtt tattcagaaa taggagctac gacaaaagtc ttgtcatagc
    37381 tcctattttt tgtcattcat ttttcaagcg gtagaaatag cctttttgag ctaacagttt
    37441 ttcgtaagta cctatttcaa cgatttttcc ttcatcgatc acaacgattt tatctgcctc
    37501 tttgatcgtt gaaagccgat gagccacaat aaaagtagtg ggtgtttctg tcaggtaatt
    37561 cagggcttgc tggatttttt tctctgactg gttatctaaa gctgaggttg cttcatctag
    37621 caaaatgatt tttggctgac gtatcaacgc tcgtgcaatg gagatacgtt gcttttggcc
    37681 accagacagc ttgttgccat gttcgcctac taaggtatcc agaccatcag gaagctggtc
    37741 aatcagtgaa gaaagttgcg cagcttcgat gacttcatct aaacgttctt ttgatacgtt
    37801 tttcaagcca taagtgatgt tctctttgat cgaagcggag aacaacaaag tggtttgagg
    37861 aacaacggct aaataatttc gcacacttcg catatccagt tcatccaaag gctgatcatc
    37921 tagaaaaatt tttcctgaag tcggctggat aaatccaatc aataagttaa ggagtgtaga
    37981 ttttccagag ccagatggcc caacaaaagc aattgtttct ccagctttca cctctagaga
    38041 aaaatgatcg ataatcggtt tgagtgagtc cggataaaga aaagaaacat cctcaaaacg
    38101 aaaatttcca tccaaatgtt ggattttttt cttaccgtga taagtttcta catcgccagc
    38161 aatcaacagt tcgccaatag aagtgataga ttccatgcct tttgaaatcg taggaatcag
    38221 cgtgatcatt ccagaaacgg aattgacgat cgtcgtgaaa tacgtctgat acatcacgac
    38281 atcgccgggt tggatgcgcc cttgtaaagc taatatccca gtgaaaatca aacaaatcag
    38341 ctgaaatatc tgaaaactag cccagctgac tgatccgaaa acagattgca ggatatctaa
    38401 gtggaatcct ttttcataga tttgagtaaa atgcttattt aagcgagttg tttcgtgttt
    38461 ttctaaccca tgagctcgag caatcggtgc taattcgatc acttcgatca cttttgcaga
    38521 agtctcttcc atttcttgcc tgaagtctga gttggactgt ctgatccttt tacggaaaaa
    38581 gagagtaacg aatgcggcga gaggaacagt caataagaaa aacatgaaaa caacaggact
    38641 tttataaagc gtgataccta aagctacgaa taaattgaca gcgatattta atccgctgac
    38701 aaatacttga gaggacaacg tttggatagc ttctacatca cggatgatct ttgactggat
    38761 tcgtccagat tgcaattctt tttgataagg aatagatagt tcttggattt tcttgataag
    38821 ggtcgctctt agtccagctt ctacttgacg gatcgaagtg ctatggtatt ttacatgaaa
    38881 ataattcgtc aatatatttt gaccaatcag acaagccatc aaaaaagcgt tgaaataaat
    38941 ggcctgcaac tgagaagaat ctttcgatga agctgcattg attacattgg caatagcgat
    39001 cggcattaac caagatggaa gatgtttgat aataaaaaag agtcctgaca tggcaaaacg
    39061 ccaataattt ccctgataaa gatgcagcaa tgtctgaaaa gcatttgcat gatatttttg
    39121 ataacttttc tgtattcgtt cctccataat cttctcctta acaatcttgg taaataaact
    39181 gcaagtgttc tattgcttca ggaacataaa tctcaggagt agcttcaaga gtggcgtaca
    39241 ttagaggacg ctcatatttg atataatgca gtaccgggcg gaaatctaaa cagcccttgc
    39301 caacaggtac catgcggatc tttccatttt ccatgatata gtctttgaga tgaagcgctg
    39361 taatataacc atgtaaattg tccaaggcac ctcggatcac tttttcctga tcagcatgat
    39421 tttctttatg catcaaattc gcacaatcta aaatgatttt taaatttggt gaagctactt
    39481 catcgattaa acgtttagct aactgatagt tatagattgg atgattgatc cctgcttcaa
    39541 cagcaacagt tacgccaaaa ttttctgcat gagcgactaa ttcaatgata gaatttctgg
    39601 cgatctgata agcttcttca gtaaaatttt cttttgtgta accgacagag acactaccgg
    39661 tttccgtagc aatcatcttg gcttgatagg cagagcacag agcaatttgc tgtttgaaag
    39721 ttgccaatgc ttgtgcacga acttcttgat ttttgctgga taggttgata tagcatccta
    39781 aaatggaaag ttcgatattg tttttttgga agtattcacc aaaataggca gcactcccaa
    39841 gtgtcatgtc agttaattta agttgttcag gaaatgcttt ttgtggtgca aattggatat
    39901 gggacaagtg aagctgtgat actttttgaa cgatttcttc acgatttcga acatctaaat
    39961 cgtgggcacg gatgcctaaa tttaatggaa acatctcaaa ctcctttttg taaataatta
    40021 gcaagaggca gacgggattc ttttatcaat tcaacaatta ttcgggcaac tttatcagcg
    40081 ccttctggac taaagtgggt attgtctttt agtccttctg ggtaattttt acaagtatca
    40141 ggtataagat gcaggtagaa tttttttgtc tcttcttttg agaaggtctg gaacaattct
    40201 tgtgatcttg agaacacatc taaaagagga atatggtgtt tctcagcaaa tgcacgcatc
    40261 gcttttggat agtctccaag tatatctgga ttcaatgttc cgttttctaa gtaatctcgt
    40321 cgcgtcacag atgtcagtaa gaccatttgg acgttcgctt tttttgcgca ttcaaaaaat
    40381 atctgcagat tttttaaata gctaccgaag ggaagcgtac ctctttcttc ttggattttc
    40441 tggtcgttat gtccaaattg tacaaataag aaatcattcg gctgaaactg tgaagctaga
    40501 cgggctaacc ttccttcctg gataaatgat ttggtgctgc gtccattctt tgcttggtta
    40561 ttgatctgga tctcgggtga aaaataagca gagatttttt ctccccaacc tgtctcggga
    40621 cgcttgtttt catctttttc tgccgcagta gaatcaccgg caatatggat agtaggcatg
    40681 ttatctcttc tttcataaag gtttataggc aatgaagcag aacagcaaga gaagaagacg
    40741 gagcaaattt ggtccgtctt cttctctaaa acgtggataa acggattccg ttatttcact
    40801 atcctgtttt tgattcgtta ctgttactat taatcaatta ggcaaaaaag ccaaagtaat
    40861 ttttagcatt tgtataacta atcgcttgaa ctagacttcc taaaagttgt tcatcttcag
    40921 gtgcttctcc tcgcatgacc atttcgccta aaaattcaca aagaacacga cggaaatatt
    40981 catgtctagt gtaggacagg aaacttcttg aatctgtcag catgccgaca aaattcccca
    41041 aagcactacc atcagcaaat tgtgtcaatt gatcgcgcat accagctcga gtatcgttgt
    41101 accaccatcc agaacctaac tgcagttttc cattctgttc tttttggaaa cagcccatca
    41161 aagctaaaat cgcaggatag tcattcggat tcaatgaata aaggatcgtc tttggcaact
    41221 gatccgtttc ttctgcacgg ttcaataatt tttgcagagg aatagtcaaa gaaagatcat
    41281 tcattccatc atagccggta tcgggcccta atttttccaa catttgtgta ttacagttac
    41341 gataagcatg gatgtgcagc tgcatcgtcc aattatgaga atgatagaag tgggtcaaat
    41401 gtatcagtgt ttctgtacga taagcatcga tttcatctga agataatagt ttgttagctc
    41461 gtgctttttg aaaaatctct tctaatactg tttcatccgc ttctttatag cgtaagatgt
    41521 ctaaagcgtg gtcggaaaca cgacatccat gttgatggaa gtattctatc cgctgcccta
    41581 agacttctat cagttcttga taggtggtga tcgtttggtc agctgcttct tccagtagtg
    41641 tgacccatgc attaaatcct tctttttcaa tattgagtgc tttatctgga cggaatgaag
    41701 gtagaacctt caaatctggt tcttcatgag caagcaactg atgatagtgc aagtcgtctg
    41761 cgggatcatc tgttgtacaa atcacttcta cattcgattt ttttgcgatt tcccgacggc
    41821 gaaaatcagg agctgccaaa atttcatttg cttgtgtcca aattttctct gcattttctt
    41881 catttatcaa caagtcaata ccgaagaaac gcttcaattc aagatgtgac caactgtaaa
    41941 gcggatttcc gataattttt ggtacggttt gacaccaagc taaaaatttt tcaaaatcag
    42001 aagcatttcc tgtgattttt tcttcaggca cgccgcaagc tcgcatcaag cgccatttgt
    42061 aatgatcgcc tcctaaccaa gcttctgtca gattgctgaa attgttgttt tcgtaaattt
    42121 cttttggatc taagtgacag tgataatcaa taatgggcat gttttttgcg taggaatggt
    42181 aaagcttttt cgcccaatcg tcttttaata aaaaatcatc agataaaaac ataaatcttc
    42241 cttctttctt tttatactgt tttatgaaaa tcaaagtgtt actccgtttt taaaaatggc
    42301 aatttctcga acgtcatttt gttcattttt cgtttcttct ccactagcta cagcgatgat
    42361 tttttgaaca aacgattcta agattacttc cattggttgt tctaataaga cgcctgcatt
    42421 aaaatccatc caatgacctt tgcgttcaaa aagagttgta ttcgtagata ccttgaccgt
    42481 tgggacatat gagccaaacg gagtccctct tcctgtggta aacaagacca actggcaatc
    42541 ggatgaagca agagcagagg cagctacaag atcattcccg ggagcttgca ataaactaag
    42601 ccctggtttt ctgatttttt cgccatattg tagcacatca acgacaggag agcttcctga
    42661 tttttgcgtg cagcctaaag acttatcttc taatgtagtg atcccacctg ctttattacc
    42721 aggagatggg ttttcgtaaa ctggttcccc gtaagacaag aaatactgtt tgaagtcatt
    42781 gatcaagtca acgattgagt caaaaacttc ttcattttcc gctcgtgcca tcaatacttg
    42841 ttctgcgcca aacatttctg gcacttctgt taagatcgtg ctgcctcctt gagcaatcaa
    42901 gtagtcagaa aatgcaccaa gtaaaggatt agcagttatt cccgatagac catcagaacc
    42961 gccgcatttc aaaccgacat tcaacttaga gagcgggact gatatgcgct ggtcattttc
    43021 cgccgctgtt aaaagctctt ctaatagagc cacaccttgt tcgatctcat cgtaaacatc
    43081 ttgagccact agaaatttca ctcgttcttc atcatattca cctaaaattt ttttgaattc
    43141 aggaactgta ttgttctcac aacctaatcc aaatactaaa actcctccgg cattgggatg
    43201 atttactgcg tccgctaaaa tttctcgagt attttgatga tcttttccaa gctgcgaaca
    43261 gccataagga tgctttaaaa tcgtaatatg gtcaaactgt cccagatctg gatgtttttc
    43321 cttgaactgt ttgacgatca gttcagcaat gccattcaca cacccaacgg taggtactaa
    43381 aaacaaatca ttacgaatcc ctacttttcc attttttcgc aagtaacctt gaaatgttcg
    43441 gttctcaaaa aggtaatgac ttggatggac atctttttca tatcgataat ccaattcacc
    43501 agaaagattg gtttttacat tatgagtgtg cagccattga ccggcagtta cgtctgtcgt
    43561 cacatgaccg attggatagc catatttgat cacgttgtct ccagctttca aatcacccaa
    43621 tgcaatctta tgaccttgcg ggatatcttc aagagtggtg atcgtttgct gatcaatcgt
    43681 cagttgtgtt tgttttggaa taggggttaa tgcgacgacc acactgtctc gtcgatttaa
    43741 tttcattgtt ggttgcaggg tttcaagcat ggaccatcgt tcctttcatt tcttgtaata
    43801 ctgaccggac acctttttga tcgatctttg cagctatttc ttgaactgta tctgtcacat
    43861 tcggtatttg agacaaatct tctccccaaa gcgcagcgtt ttgcaataat cgaggaatag
    43921 ctgttccatt atcagaccaa gcatcagtaa attcgcttat cgtcttttca tcatcatgtg
    43981 gctttacttg atcgcctcga tacataagaa gtagcgcagc tagtgaaaca gtgattaacg
    44041 gtggtacctg attttgttct tcgatatact tcttgaacac tggaagcaga cgggtgctga
    44101 actttgaaac actatttaat gaaatcgctt ctaatttgtg atgggcaaaa ggattcaaga
    44161 aacgttcttt gatttggtca gcataaatgg ctaattcatc tttaggtaaa gaaagcatag
    44221 gaatcaattc tagattgaat aactgatcaa catatgcgga gaataaagga tctttcatta
    44281 cttcttctac agtttccaat tccgccaaca aagcaagagg gaccatcgct gtatgcggtc
    44341 cattaagcaa atgcacctta cgttcacgat aaggagtcat atcatctgtg atgatgacat
    44401 tcaagccagc ctgtttgaat ggtagttgat tctccaattc tttaggccct tcgatcaccc
    44461 aaagcatgaa tggttctgct ttgaccatca attgatcttc atagttatgc cgttgattga
    44521 aaacacctgc atcttcgcga ggataacctg ggacgatgcg atcaactaaa ctacaacaga
    44581 aaacattttc tttttctaac cactcaacaa aagcaggttc taaatcccat ttttctgcgt
    44641 agcgtaaaat gatttctttt agacgttcgc cgtttctgtc aatcagttcg caaggaatga
    44701 tggtaaagcc agctttgttt aatttgaaac gtcggtataa aaaggctgtc agtttgcccg
    44761 ggaagctctg tgggggagta tctgttaaat tatcagtctc cacgaattgg attcctgcct
    44821 ctgttgtatt ggagacgata taggccaagt cgtcattttc tgctagagat agataagcat
    44881 cccattcatc ataaggattg atcatttgtt ctactacggt gataatctcg gaagtatcaa
    44941 cgatttcgcc attgctgagt ccttgtaaaa tgaccgtata taaattatct tgctggttca
    45001 tcatatttcc aagaccttgt tctaaaggtt gaatgattgc aacattacct ttaaacaatt
    45061 gttggttatt cagctgctga atctgccaat caataaatcc acgcataaaa ttgccttcac
    45121 caaactgaag aacttttaca ggtgcttcgt tttttttcac taattctttt gtcagttttt
    45181 tcatataaac ttcctttctg tgttatgcac acgttaacat ttttatataa aattacttgt
    45241 agtttacaag taattgatcg actcacgaag gatcaacaag gtggagagct caattttaga
    45301 ttgttcgatc gtcctgcctt cgatcaaatc tagtaaaagc tttgttcctt cttgacacat
    45361 ttctttcaaa ggcttacgta cggtggtaag cggcggattg agataggaga cgactggaga
    45421 atcatcgaac ccgatcagag aaatgttctg gggaacacgg aggccagcta aatgtacagc
    45481 tctcaatgca ccaatcgcta tgtcgtcgtt gccgcaaaaa accagagtag gcaggtcaga
    45541 aagtgataat aggtattcca tgttttggag gccgctttct aagctgtaat ctcctgttaa
    45601 aaaataggct ggattgacag ggatgctcgc tttttttaga cttttcaata cccctttttc
    45661 ccgttctttt gtcgaacgaa aatttttctt tccgccaata taagcgatct tcgtatgtcc
    45721 gcatccaata ccgtaatcta ctgcctgcat aacaccggcc gcatcatcgg cagtgacgtt
    45781 gataaccgct ggatcattga tgtgacgatt gacaacaacg aaaggaaatt ctttttcttt
    45841 gagatagtcg atgaaaggct gatcatcatc actttgactc ataacgatca gtccatcgta
    45901 gcgttgcagg ttgatttctg ttaaatcaac aacatcgata ctttcaacag ataaactgta
    45961 atttgtgtct aatactgaat ggatgcctgc aatggaatct gctaggaaat ttgatgaagt
    46021 tcctttatgc aaacttgaga aaaatagtcc gattagatat tttttttgat tcactaaacc
    46081 ttttgcacta tagtttggcg aataattcat ttccttagct agttgtcgta ttttttgttt
    46141 tgtttcattt tttattaaac tgctgttatt caaagcacga gaaactgtcg tatgagaaac
    46201 gccagctgct ttagcgatat ctttgatcgt taccattcca tcattttcct tactttttca
    46261 ctttttgtta acgcattcat catattacga taactacgat aagtcaatag atctttgata
    46321 tgaaaagatt gcacacgtta aaaattactt ggatttttat ttttaattat gctattcttg
    46381 aaagcgggga atgctcattt aattagatga gtatcaattt tacagaaaaa tgaaagcctt
    46441 ttattttaaa aggaggagag gaagaaaatg aagaaaagta cagtatttta taaaatattt
    46501 ttaccgatgt ttttattagg agccgtactt gtcataggat ttagtttatt catttaccag
    46561 aatacctatg aatcgatcga atcgagttat ttaacagaca aaaagaattt gctcaggcaa
    46621 gttaaaacta acgttgaatg gaaaattaga acgattgaat attcgttttc tacgtatggt
    46681 tcgaccaaaa atttctcgga catattcaag catccgctga catatacaga ttactccact
    46741 tattctgaaa tcaggaaaga actaaatttt atagaaacga ttgttatgga tgataataat
    46801 tatgatttag tcagtttggc tggtaaatgg ggagtaatca atggatcgtt gagtcaactg
    46861 accgaagagg aagttgaaaa gtatcaacag aagtacataa ataacaaaaa taatttattt
    46921 tggcaaaaga aaaacaaagg aatcgaaatg gtgatttctt tgccaatgtt tgaaaaagaa
    46981 aaatatgcat tgggaattgc tacgatagag aaacatacaa tagaacaagt tgttggtgaa
    47041 agtggagaag acatactttc tattgaaaac gaagttggca acctttttac tactcagaca
    47101 gtaaaaaata aagagcaacc aactgtatta acggacgtgg atctagatta caatcagctg
    47161 aaagtgataa gagaaaaaga taaaacgtat atagtactga aatcagatta taataattgg
    47221 atttacagat tagaagttga ctctagtgca attggcgcaa tgatccgcaa tctgagaatc
    47281 gggctagttg cagtgtcttt aacactggta ggactcattg gtcttctttc ctacttattt
    47341 tcggacagat ttgcccgtcc gatttctcaa atacaggaac gtctgaatct taaatcaaaa
    47401 ggattcagtg ggaaagaact agatttggtc gctcaatcag tcagtcggat cataggagaa
    47461 aaagaagcgt taagtgcaca cttagttacc caaaagccac agctggaaac gctatttgta
    47521 cttagtttat ttagaaatcg agtagaaagg cgagaactag atcagcggtt gcagcaattt
    47581 ggttatagtt ctcaaaatga ctgctattat acagctttgg ttcaaatcga tctgttagaa
    47641 gatagtcacg gtggggagcg tgatttgcta cttttagcga tcaacaacat gatcacggaa
    47701 attgtgccaa aggaaaatcg aatgctgccg atcgttttga atgaagaaat gcaagccact
    47761 atttatcgaa tggataaaga tgatgcagaa gcaagcaaaa aagtcatgga gtattgccga
    47821 ctcattcaaa aacgtgtaaa agaatacttg aatttaacta tcagtatagg catcagtaac
    47881 cggttcgaaa cgctgaatga aagcaagcaa tcggttgatc gggcaaaaga agccctgtat
    47941 tatcgtgtaa atactggtcc aagttcaatt attttttatc aggaaatcgt tcctgctgct
    48001 catgaaaaaa cacttattag atatccgata gaagaacaaa atcgattgtt tgaagccatt
    48061 cgttctggaa atcaggaagt gaagaattta gtacatgaat tgattgatgc attgtttgca
    48121 caaaataaaa atcctttgag tcgggaagta gtttcggtcc gcttgatcaa tgaactcgtt
    48181 caattaggac agttattggg agtagattca aaaaactttg ataacatgaa acaaatttat
    48241 gtaaaagcac tgaacaatta tcatcccaaa gaactagaaa aacttgtttt agaacaatta
    48301 gttctgccaa tcacagaatg ctctcaagca atcactgatc atgaatttaa aaatttgtcg
    48361 gagaatatta tgcatatcgt ctacacagaa tatgatcgtg atctgtcttt agatgtgatc
    48421 gctgataggt tgcattataa ccctaactat ttaagtaatg tatttaaaaa ggaaacagga
    48481 gaaaaatttg gtgattttgt tcaaaatcat cgtttagagg tagcaaaaaa atggttgaaa
    48541 gagactaacc tctccgtaaa agagattgca gaaagattgc aatatcgaaa tccgcaaaat
    48601 tttattcgtt tctttaaaaa gaaggaatcg attacgccgg gagaatatcg aaaaaaatat
    48661 ttgtgaatca agaggctgtt tttcttgtgg actgattcaa gcaataaaag aagttagaat
    48721 aaccctagta tttgtattta tggatagtta aatttcaagg ctaagcacgc aaaaagaact
    48781 gtgacaaaag tcttgtcaca gttctttttt acgaataaat ggcgttcaag agacaggcta
    48841 tcgacaataa ggcataattt tgtgaaatat gggaagctat ttttcaaaat cgcttcttat
    48901 tgctcctgtg ggttactcgt cgcaatgttt gtgagagttt ttatcatcgc tcaaggctga
    48961 acgtctcttt cacaacctct gatacacggt gttccaggag tagattcttc gaaaaataag
    49021 ccgaaatttc acaaaaattt aagaagcaat tttcgtaaat gccttcttat ttcttgaagc
    49081 tgaccacttc tgacacaact accattttgc gttcagtaca taatcaccct ttgacagaac
    49141 ctacgagtgc tcctttgaca aaatatttct gaacaaaagg ataaacaatc agcataggca
    49201 ctgttgcaat aacgataaca gccattttaa ctgtttgtgc tggtggaatc acatcaacgg
    49261 cactactttc tgcttgcatc ccacttgata caatgacgat ttggcgtaaa agaatctgta
    49321 tcggccattt agatgaatcg ttgatataca aaatagcagt catataagtg ttccagtaag
    49381 taacagcata aaaaagcgag atagtagcaa tagacggtaa cgctaatggc agcataattt
    49441 tgaaaaatac accaaaatct gtgcaaccat ccatttttgc tgattcttcc aaactatccg
    49501 gcaaggcttg gaaaaagttt ctcataatga tcatattcat tgcattaaca gccacaggca
    49561 ggatcatggc ccagtaagaa ttgaccagcc ctagagaacg aacaactaag aacgttggaa
    49621 tcatcccccc gctgaacaac atagaaaata caacaagaaa gttgacgaaa cctcgaccaa
    49681 acaaatactt tctggaaaga ccgtaggcca tcagtgaagt tgcacacata ctgacgatcg
    49741 ttccgacacc tgtaacacca atcgaaacag caagtgctct aaaaatggtc ggcgtagaaa
    49801 gaatgtagcg atacgcgtct aaagaaaagg tagtaggaaa aaggataaac tttttcgcga
    49861 cgacttcttg tgtagaagca aaagatgtag cgatgatatt taaaaaagga acgatacaga
    49921 tcaaagctag caaaattaga aacgtattgt tgaaaagtgt aaaacttctt tctcctatag
    49981 agactttctt tttcatatag ataggctcct cctcaaaata aattaaatgt ttctgtttct
    50041 atatgttcaa tttatcatcg gtgaaagatt acgtatataa tcatttccta tgctaagtgt
    50101 cagatagaaa aaaatagaag gtttgctatg cgtagtttat ttgtgaaaaa atacacttta
    50161 tcgttttgta tagcacaaaa atcattgcta tgtaagcgtt tttacttacg gtaaaaatga
    50221 ttatatacgt aattcagata gttatctatc atgaagtcat aagcagcaaa cattcacaaa
    50281 actaaggagg ggcttatgga aagtgtaaag aaacaagaac catttcaact gacgctatcg
    50341 aaaaaagaag aaaagctcag aagacgaaaa aaaataattg caggaatcaa aacgaataaa
    50401 tttttatatg tgatgattct gccgggagtc ctttatttct tgatttttag gtatcttcct
    50461 atgggggact cgttattgct tttcaagatt atcaaccatt tttaggaata atgggaagtc
    50521 cttgggtcgg tttcaaacat ttcattcgtt tgtttacaga accgacattc tttacattat
    50581 taagaaatac gttgatcttg ttcggattaa atatcttcgt gttttttccg atgccaatca
    50641 ttttgtcttt actattaaat gaagtaacga acaaacgatt gaaaaacggg attcaaacga
    50701 tcatttacat ccctcacttc atgtcttggg taatcattgt ttcgattact tatgtatttt
    50761 tgaatgttga cggaggggta ttgaatgaat tccttgcaac aatgggattt gaaaaaatca
    50821 gttttctaac atcccctgaa tggacacgca cagtatatat cggacaagtg atctggaaag
    50881 agttgggctg gtctacgatc atctaccttt cagcgataac tgcagtcgat acacagttgt
    50941 atgaagctgc agaaatggat ggagcaggac gcttacgcaa aacatggcat gttacattgc
    51001 cggcaattcg tccagtaatc attaccttgc tgattctaaa aatcggtcag acattagact
    51061 tagggttcga gcatatgtac ttgttattga attcattgaa tcgaagcgtt gcagaaattt
    51121 tcgacaccta tatttataca gctggattga agaatggaca gttaagtttc agtacagcaa
    51181 tgggattgtt taaaggggtc gtaggattgt ttatggtaat tttagctaat aagattgcca
    51241 aaaagctagg agaagacggc gtttactaag atagaaagag agggaaagat atgctgcagt
    51301 cattagataa aataaacgga aggctaaatc gtattttaca gttggtttat ttgaattttc
    51361 tgtggggcgg tttagtcatc gcaggtttgg gagtttttac tttcggtcct gcatcgtatg
    51421 cgatggttgc agtgattaga aaatggctgc gctcaaaaga aacgtttcct ttgacgaaaa
    51481 cgtacttcac atattttaaa gaaaactatc gtgaaacctt gctgatgagc tggatatatg
    51541 gcgccatcgg gattgtttta cttgtggatc tttctttcat acacaattgg tatcttagga
    51601 tcgctttgta tattgctgcg ttcatatatg tgctctcagc actctatatt ttcccaatca
    51661 tggctcatta tgattggaaa ggatattttt tcaaaatgaa aatggcattt ttattcggat
    51721 tctcacatct tcattactca ttagtgttga tattaagtat ggtagggatt tatctgatga
    51781 tcatccaaac aatgccaggt gttttaacct ttatcggtgg cagtctcttc ttttccgcag
    51841 tcacgtggac agcaaattct atattttgcc atttggaatg gaagaactca tatacagaaa
    51901 aaacagaaga aaacaaaaat attattaaaa cagtgaagga gaaaatttat gaaaaaagca
    51961 acgctcgtaa aattaggtct tagttttgca ggaattctag cacttgccgc atgcggcagc
    52021 agttcaagta acagttcttc agagacagaa gatggaaaag ctaaaatcac ttggttaaat
    52081 attttgcatc atgcttcacc gcctacagac acgattgtga acttgatcga agaaaaaacc
    52141 gatacggaac tgacattcaa ctggattcct gatgcttcaa aagaagaaag attaacaact
    52201 gcattggctt ctgatgaact aggagatatc gttacacttc aaattgcgat gttgaaaaac
    52261 tcttctgtcc gcaatgcgtt aaaatcaggc gtattctggg atgtatcaga atatttaaaa
    52321 gactataaaa atttaagtca aatttctgaa gatagaatcg aagctgcgag tatcgacggt
    52381 gccttgtacg gcgtaccgat acaaaaagac tacgctcgtg ctggcttagt cattcgaaaa
    52441 gactggttgg ataatctagg actagatgtt ccaactacca tggatgagct ttatgaagta
    52501 gctaaacaat ttacagaaaa cgatcctgat ggaaatggtg tggacgacac agttggtttt
    52561 ggcgatcgtg cgcctagtga tattcgttat accagcttta aacttttcac ttcttatttt
    52621 ggagcaccaa atggctgggc tgtagacgat aatggcaaat tcactccaga atttgataca
    52681 gaagagtata tgaaagcttt gaatttttca agagatctat ttgagaatgg ctatttggca
    52741 caagactttg cagttaccca aaagactgat cagcaagaac aatttgcaca aggaagaact
    52801 ggaatctaca ctggaatggt ggatatcaag aatctgaaga cgatgggtca aggtattcaa
    52861 gatgatatgg aattagtgcc tgtaaacaag atctcagatg gtgaaggcgg cggttatcat
    52921 gtttggtcag aaggaaatgg tattggcggc ttgctagcat ttcccaaatc tcaagtaaaa
    52981 gacgaagcac aattgaagaa attacttcaa tttgttgatg atcttttaga agaagatgtg
    53041 tataaattga tgactggcgg tatcgaagga acgcattatg aattgaatga tgatggttct
    53101 atcgaattta tcgatatgga tctttggcaa caagaggtgc aaccgttatc aagctctcgt
    53161 cctagtgaag tgacgttctc tttcaaagat acagaccctg aaaaagaatt ctctaatcaa
    53221 ttgatcaaag aaaatgaaga atttgctgta atggatcctt ctgtgccatt ggattcaccg
    53281 acgaacaatg aagtaggatc agaaattgaa aaaatcgtca ttgatgctac ggtgcaatac
    53341 atcatgggac agcttgacga agatggattc aaaaaagctg tagaagactg gaaagcacaa
    53401 ggaggcgatc gaatcacaca agagtacgag gaagcataca aagcagcggc acagtaatat
    53461 agacgattgg gacaaatact tcttggtatt tgtcccaaat cctattttaa tttttaaagg
    53521 agagcaaata atggaaaact ggtcaaagaa attagcaaaa agtatcatgg aaagaacacc
    53581 tcgcttatat gaagaaaagt ggtataaagg aaaatggtct tatgactatg gcgtcgtact
    53641 taaagggttc caattacttt gggaacgaac ccaagaaaaa atttattttg atttcataaa
    53701 agacaatatc gactatttcg tgcaagaaga tggaacgatc cgtggctaca gcgttgaaga
    53761 atacaatatc gaccatgtaa atacaggaaa gcttttcttt ttattgtaca aagaaacagg
    53821 cgaagaaaaa tataaaaaag cagcagaact cctttcccag cagttggcaa accatccacg
    53881 cacatccgaa ggagcttttt ggcataaaga aatttatcct tatcaaattt ggttggatgg
    53941 cttatatatg ggttctcctt tttatgctga atttatccag aattttagtg aagaaaaaga
    54001 ttaccaagat gttttgagac agtttgaaat cagttataaa catctattcg atccaaaaac
    54061 aaatttgctg attcatgcat gggatgaaaa gaaggtccaa ccatgggctg atccaaaaac
    54121 aggtctatcc catcattttt ggagccgatc gattggctgg tatttgatgg ctgcagcaga
    54181 tacctatgaa ttacttcatg aagaaacaga ttgttctttg ctgaaagaaa ttctagaaaa
    54241 gacactggct gcattacttc catatcaaga agaaaacagc gggacatggt atcaagtaac
    54301 aacagaaaca aaacggaaag gaaattatct ggaagcttcc ggcagtagta tgtttgttta
    54361 cgcaatggct aaaggaatac gtttgggact gctgaacaaa gaagaatggc ttccacaagt
    54421 gaagaaatca catcaaggat tacttgatga atttgttctg gaaacaaaag aaggctggct
    54481 gaacttgaat aaaaactgtc aagtagcagg gttaggcgga gcagatcagc gagatggttc
    54541 atatgcttac tacatcagtg aaccaattat ttgtaatgat caaaaaggcg ttggtgcatt
    54601 cttgcaagca ttgattgaag tggaggcatt ataaatatgt atgatatttt gacatttggc
    54661 gcatcaattg atgaattgaa tactgaagca atacaacaag ccattgatgc tgcagcaagt
    54721 gacggaggcg ggaccgtagt tgttccagca ggtgaatttc taacaggagc tttattttta
    54781 aaaagcaatg tcgaacttca tttaagcgca ggagcggttc tgaaattctc tgacgatcca
    54841 aaagattatc ctgttgttca ttcacgttgg gaaggagtcc atcgaaaagt ttatgcttcc
    54901 tgtatctatg ctcaaaatgt agagaacatc tctgtcactg gtttcggtac attagatgga
    54961 aatggaaaaa aatggtggca tacttttaga aatgaaccag ataatctcgc ttatcctcgt
    55021 cctaaattga tgagtttcca taactgtcat cgaattactg tgaaagatat taaattgatc
    55081 cagtcaccaa gttggacgat caacccgatt ctttgcagta atgcaacgtt tgataacttg
    55141 acgatcttga acccagcgga ttcaccaaat acagacggaa ttgatccaga atcatgcaaa
    55201 aatgtgcgta ttagtaattg ccatattgac gtgggagatg actgtattgc aatcaaggca
    55261 gggactgaag atacgtacga aaggatcgct tgcgaaaata tcacgatcac aaattgcacg
    55321 atggtccatg gacatggcgg tgtcgtatta ggaagtgaaa tgagtggtag tattcgtaat
    55381 attaccattt caaattgtat cttccaagaa actgatcgag gaatacgatt gaaatctaga
    55441 cgaggtcgag gcgggatcgt tgaagatatt cgagtatcca atatagtcat ggataatgtg
    55501 atgtgtccat ttatactgaa tctttattat ttctgtggtc cgcgagggaa agaaccgtat
    55561 gtatgggaaa aaaaggctta tccgatagat gaacggacac cagcattcag aaggatccat
    55621 ttttcgaata tcacggcaag gaatgtccat gcttctgcag gttttattta tggattagcg
    55681 gaacaattca tacaagaaat cacatttgac aatatcgatg tctcgatggc ccaagatgca
    55741 gttcccggtg ttcctgctat gatgacagga atagaggaaa tgagcagaca aggattttat
    55801 attggctttg cgggtaaggt ttcctttaac cagatgtcga ttgaaaatca cgagggacca
    55861 gcatttctat tagaaaatac taaagaaatc agtattgaga aattccaatc aaatcaatca
    55921 gaaaatatgg atgaactggt tgttcgtaaa aactttgctt atgcagttga atgatttttt
    55981 aggtgctcca tctagcagac atacccctag tggaaaacga ttataccgag aaaaacaaaa
    56041 cggttttatg cttgaatact atcaattgta cttaaatgaa ttggaaacag taccagccct
    56101 ctatgcttat ccagaaaaag aaggaccgtt tccgccggtc ctttacctgc attctcacgg
    56161 cggagatttt tcagtcggga aaagtgaatt gatccatggt gctccttact tagcttctcc
    56221 atcttttgca gaagtactga caggaatggg atatgctgtt tggtcgattg atgcttgggg
    56281 atttgaagaa cgcggaggta tttctgaaag cgagttgttc aaacaatttt tattaacagg
    56341 aaaaacgtta tggggaatgc gtatttatga tgtgatcagc ttgatcgatt atttggagac
    56401 aagggaagac acagatactc aacggctcgc aacgattggt ctttctatgg gaggcttatt
    56461 aagctggtgg gtgacagcat tggattctcg tgtcaaagtt tgtattgatc tagcggcaca
    56521 ggtggacatc gaaatacttt tgctacatcg ccgcctagat catcatgggt tttattacta
    56581 tgtgccaaat ctattgacag cttatacaac gttagcaatc caagaaaaaa tcgctccgcg
    56641 ccctcgtctt agcttagttg gaaaaaacga tacgatgtgc ttggacgaag gcgtactgaa
    56701 gctgagaaaa gggctggaca aaaagtatca atcaatgggt agtccagaaa acttttcttc
    56761 tttttcttta acaggcggtc atatggaaac acaagaaatg agaaatattt ggaaacaatt
    56821 cataagggag catttatgag gatgaaagac aaactgataa ttggaaagaa tgctatagat
    56881 accccgacgg attttgaaac tatccaagaa ggaatcgatt atttggcagg cctgccggat
    56941 agccatccaa aggtcttgat agtaaaagaa ggaagatatg atgaatatat agaaagcaga
    57001 ttatccaatt ttaaacttgt gggagcagga acagtagtta tctccggtaa tcgttttgca
    57061 aaacaggagt atcttgacgg tagccagtta gggactttcc gaagtgctac ttttttctta
    57121 gaaggatcga atgtcgtatt agaaaatctt cacatcgaaa atacagcagg tccaggagaa
    57181 aaggtaggac aggctgttgc tttattcaat tcaggagatc gcgtatcttt aaaaaactgt
    57241 cgactatctg gttatcagga taccttatgc acgggtccgt tgcctgattt gcaaaaagat
    57301 ggtactcctt tttgtacacc ggcttctgcc aatcctgagt attgtcgtca gacctatgaa
    57361 tattgctgga ttgaaggaac ggtggatttc atatttggtg gggcagatgc cgtttttcat
    57421 cagtgtcata tccatagctt agccagtaaa aatcctggat acatcacagc agcatctacg
    57481 ccgaaaaatc aaaaaacagg gtatcgtttt gaaagctgta ccttgactgc agaacctgat
    57541 actagaaacg tctatttagg aagaccgtgg cgtccgtatg caaatgtctt atttgaagcg
    57601 tgtaagatag gtgggcatgt tcacccaact ggttgggata attgggatga caagaaaaat
    57661 gaacagactg tcaaattctc tgaacgtaat aacaattatg aaacatcgat caaaagacct
    57721 gaatggatac atttagaaaa gcagggggtt cagaaatgaa aagaaaatgg gaactattgc
    57781 taggaatggt cggtggcagt ctatctttga ttttctttgg cgggttagca gttactttaa
    57841 gtaatatgtc tgcgtcagag tttaaaaaaa gttatcagtc gcttgctgta gatcattcaa
    57901 ctttgtcttt agaaaataca tttggattat tacaggacat gactggtctg tttgccgttg
    57961 tgttgtttat ttcacttgct tttttagcag tagcattgtt tttgactgct aaaggaaaat
    58021 acttaacaac tgccactggt ttgtatttta taacaggatt catattgttg attggcactc
    58081 agtttattgc gtttcctttt gcattttttt atttcgcagc tggggcattc agtttatatc
    58141 gagtaagaat gagaaaagga gcctgaatca tgcagactat caaagtgaaa aatcctattt
    58201 taagaggatt caatccagat cctaatatat taagagtgaa agagcggtat tacattattg
    58261 tctcctcttt tgaatggctt ccaggaatcc gagtatatga gtcgaaaaat ttagtcaact
    58321 ggtcacatac aacggatatt ttgaccactc aagtatcttt gcaaggaaat cctacaaatg
    58381 gaagtatatg ggcgccacaa atcagttatg ctgatggatt gttttatctt gtttacacag
    58441 atgtcaaaag tgcaggccgt ccgtttaaag atgtccacaa ttatttgatc actgcatctg
    58501 agatcacagg tccttggtcc gaaccaattt atttaaatag taacggcttt gatccttctt
    58561 tgttccatga tcttgaaacg ggaaaaaaat ggcttgtcaa tgaactatgg gattatcgtc
    58621 tggacacacc gaataaatcc agcggtatcg tattacaaga atatgatgca gagaagaaga
    58681 agcttgatgg tcccatctat aaaatatttg caggtacaga attagcgaaa acggaggcac
    58741 cacatttgta ttttcgtaac gggtattatt atttgatgac ggcagaaggc ggtacaggaa
    58801 gaggacattc agttacagtt tgccgagcaa agaatattac tggtccttac gagcttgatc
    58861 ctggttttcc tatgctgaca gctagtgaca aacctgcttc tacattgcaa tgtacaggac
    58921 atgggagctt agtacaaacc ccgtcaggaa gatggtacat gacatatctt tgtaccagac
    58981 cattaaacgg tgcggctatt ttgggaagag aaaccgctat tcaagaagtg tattggacgt
    59041 ccgacgattg gttaagactt aaagatagcc agacaactcc gatggaagaa ataatgattg
    59101 agacaaatga agaggtacaa caagaaaaag atcatcagtt tatggataca tttgaaggag
    59161 agctaaagaa gaactggaat gccagacgca ttttgccaaa tgctgattgg tgtgatacaa
    59221 aatgtagacc aggtttttta cgtctgattt ccggtgaatc tcttcaatct accttcgaac
    59281 atcatttgtt agctattcgg caaacggatt ttacttttga tgcggagaca gcatttgaat
    59341 acatgcctaa taattttaat cagatggctg gactggtact gtacttaaat gaatccaatt
    59401 atatttattg ttatgttact tggaatgagg acaagggaaa atgcctacgt atgatagaat
    59461 cagaaaacgg agtagcacaa atagaagatt ggacgattcc actcacagaa aagaagactt
    59521 ttttgaaagt cactgtaaag aaagagacag cacagtttca tttgaaagac gtatcaactg
    59581 aagaatggaa gaaagtaggt ccgcaaaaaa acatgtgtat actctcagga gggtttactg
    59641 gaaattttat cgggatagca gtgcatgatc taaataaaaa aagaggaagt tatgctgatt
    59701 ttgagttttt caagtatgat ggaaaagatc atctgtaatt taattcttta ataaatgagg
    59761 ttcactaact tattttcact ag
//
LOCUS       DACCZY010000003        55285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCZY010000003 DACCZY010000000
VERSION     DACCZY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.003"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            4..861
                     /locus_tag="IVZ67_000098"
     CDS             4..861
                     /locus_tag="IVZ67_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8164204.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="IVZ67_000099"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="IVZ67_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8164205.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="IVZ67_000100"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="IVZ67_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8164206.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="IVZ67_000101"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="IVZ67_000101"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8164207.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /locus_tag="IVZ67_000102"
     CDS             3617..4375
                     /locus_tag="IVZ67_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8164208.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="IVZ67_000103"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="IVZ67_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8164209.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5303..5602
                     /locus_tag="IVZ67_000104"
     CDS             5303..5602
                     /locus_tag="IVZ67_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164210.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5628..6335
                     /locus_tag="IVZ67_000105"
     CDS             5628..6335
                     /locus_tag="IVZ67_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8164211.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6332..7099
                     /locus_tag="IVZ67_000106"
     CDS             6332..7099
                     /locus_tag="IVZ67_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8164212.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7199..8767
                     /gene="liaX"
                     /locus_tag="IVZ67_000107"
     CDS             7199..8767
                     /gene="liaX"
                     /locus_tag="IVZ67_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8164213.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9067..9384
                     /locus_tag="IVZ67_000108"
     CDS             9067..9384
                     /locus_tag="IVZ67_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8164214.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9384..9734
                     /locus_tag="IVZ67_000109"
     CDS             9384..9734
                     /locus_tag="IVZ67_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8164215.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9901..10839
                     /locus_tag="IVZ67_000110"
     CDS             9901..10839
                     /locus_tag="IVZ67_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8164216.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10854..11684
                     /locus_tag="IVZ67_000111"
     CDS             10854..11684
                     /locus_tag="IVZ67_000111"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8164217.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11728..12753
                     /locus_tag="IVZ67_000112"
     CDS             11728..12753
                     /locus_tag="IVZ67_000112"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8164218.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12768..13706
                     /gene="galU"
                     /locus_tag="IVZ67_000113"
     CDS             12768..13706
                     /gene="galU"
                     /locus_tag="IVZ67_000113"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8164219.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13831..14271
                     /locus_tag="IVZ67_000114"
     CDS             13831..14271
                     /locus_tag="IVZ67_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8164220.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14274..14876
                     /locus_tag="IVZ67_000115"
     CDS             14274..14876
                     /locus_tag="IVZ67_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8164221.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14944..16050)
                     /locus_tag="IVZ67_000116"
     CDS             complement(14944..16050)
                     /locus_tag="IVZ67_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8164222.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16260..17261
                     /gene="ccpA"
                     /locus_tag="IVZ67_000117"
     CDS             16260..17261
                     /gene="ccpA"
                     /locus_tag="IVZ67_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8164223.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17564..19909)
                     /locus_tag="IVZ67_000118"
     CDS             complement(17564..19909)
                     /locus_tag="IVZ67_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8164224.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20188..21090
                     /locus_tag="IVZ67_000119"
     CDS             20188..21090
                     /locus_tag="IVZ67_000119"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8164225.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21303..23078
                     /locus_tag="IVZ67_000120"
     CDS             21303..23078
                     /locus_tag="IVZ67_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8164226.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23335..24552
                     /locus_tag="IVZ67_000121"
     CDS             23335..24552
                     /locus_tag="IVZ67_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8164227.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(24999..26585)
                     /locus_tag="IVZ67_000122"
     CDS             complement(24999..26585)
                     /locus_tag="IVZ67_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8164228.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26836..27060
                     /locus_tag="IVZ67_000123"
     CDS             26836..27060
                     /locus_tag="IVZ67_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8164229.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27202..28953
                     /locus_tag="IVZ67_000124"
     CDS             27202..28953
                     /locus_tag="IVZ67_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8164230.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            28953..30737
                     /locus_tag="IVZ67_000125"
     CDS             28953..30737
                     /locus_tag="IVZ67_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164231.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31195..32046
                     /locus_tag="IVZ67_000126"
     CDS             31195..32046
                     /locus_tag="IVZ67_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8164232.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32143..34029
                     /locus_tag="IVZ67_000127"
     CDS             32143..34029
                     /locus_tag="IVZ67_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164233.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34070..34972)
                     /locus_tag="IVZ67_000128"
     CDS             complement(34070..34972)
                     /locus_tag="IVZ67_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8164234.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35202..36605
                     /locus_tag="IVZ67_000129"
     CDS             35202..36605
                     /locus_tag="IVZ67_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8164235.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36605..38038
                     /gene="ascB"
                     /locus_tag="IVZ67_000130"
     CDS             36605..38038
                     /gene="ascB"
                     /locus_tag="IVZ67_000130"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8164236.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38183..39109
                     /gene="trxB"
                     /locus_tag="IVZ67_000131"
     CDS             38183..39109
                     /gene="trxB"
                     /locus_tag="IVZ67_000131"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8164237.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39322..41049
                     /locus_tag="IVZ67_000132"
     CDS             39322..41049
                     /locus_tag="IVZ67_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8164238.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41122..41415
                     /locus_tag="IVZ67_000133"
     CDS             41122..41415
                     /locus_tag="IVZ67_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8164239.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(41499..42359)
                     /locus_tag="IVZ67_000134"
     CDS             complement(41499..42359)
                     /locus_tag="IVZ67_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8164240.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42451..42879
                     /locus_tag="IVZ67_000135"
     CDS             42451..42879
                     /locus_tag="IVZ67_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8164241.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43041..43217
                     /locus_tag="IVZ67_000136"
     CDS             43041..43217
                     /locus_tag="IVZ67_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8164242.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43244..43690
                     /locus_tag="IVZ67_000137"
     CDS             43244..43690
                     /locus_tag="IVZ67_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8164243.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43846..45225)
                     /gene="brnQ"
                     /locus_tag="IVZ67_000138"
     CDS             complement(43846..45225)
                     /gene="brnQ"
                     /locus_tag="IVZ67_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8164244.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            45931..46902
                     /locus_tag="IVZ67_000139"
     CDS             45931..46902
                     /locus_tag="IVZ67_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8164245.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            46926..49118
                     /locus_tag="IVZ67_000140"
     CDS             46926..49118
                     /locus_tag="IVZ67_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8164246.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49135..49611
                     /gene="ybeY"
                     /locus_tag="IVZ67_000141"
     CDS             49135..49611
                     /gene="ybeY"
                     /locus_tag="IVZ67_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8164247.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49589..49990
                     /locus_tag="IVZ67_000142"
     CDS             49589..49990
                     /locus_tag="IVZ67_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8164248.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50005..50904
                     /locus_tag="IVZ67_000143"
     CDS             50005..50904
                     /locus_tag="IVZ67_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8164249.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51047..51859
                     /gene="recO"
                     /locus_tag="IVZ67_000144"
     CDS             51047..51859
                     /gene="recO"
                     /locus_tag="IVZ67_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8164250.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52243..53160
                     /gene="glyQ"
                     /locus_tag="IVZ67_000145"
     CDS             52243..53160
                     /gene="glyQ"
                     /locus_tag="IVZ67_000145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8164251.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53162..55237
                     /locus_tag="IVZ67_000146"
     CDS             53162..55237
                     /locus_tag="IVZ67_000146"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8164252.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17908 a         9926 c        11767 g        15684 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagccattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggacagggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 gtcacaacga tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc atatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcaa tctaagtttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcgggaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa
     5101 gaggttgtga aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca
     5161 aacaaaaaat agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct
     5221 tttgaacacc gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat
     5281 catttcatag aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat
     5341 ttgtgtgctt acttcatggg ttattcagca tcaagctgct ttatatgaag tacctgtcat
     5401 cgagatcgaa aaagtacgaa taaaagagga tcatcaattg attacaggaa gactgatgaa
     5461 cacggatcaa aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga
     5521 aacagcaaat ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca
     5581 gctagtagca aaaaaaaaat gatggctggc ttttcttctt cacaagtatc tccattggcg
     5641 ccatgttgct tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
     5701 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
     5761 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
     5821 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
     5881 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
     5941 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
     6001 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
     6061 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
     6121 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
     6181 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
     6241 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
     6301 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
     6361 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
     6421 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
     6481 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
     6541 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
     6601 cctactattg cttcctatca ctgatttttt agcgatccaa ggttttacat cggtagaact
     6661 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
     6721 gttgcttctg attagtctta gtggtgctgt tttagatgct agtgtagcaa tttctagtgg
     6781 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
     6841 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
     6901 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
     6961 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtctgc
     7021 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
     7081 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
     7141 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
     7201 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
     7261 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaataa aaaaagaagc
     7321 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
     7381 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaaggcaaga
     7441 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
     7501 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
     7561 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
     7621 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
     7681 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
     7741 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
     7801 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
     7861 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
     7921 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
     7981 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
     8041 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
     8101 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
     8161 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
     8221 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
     8281 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
     8341 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
     8401 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
     8461 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
     8521 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
     8581 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
     8641 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
     8701 tgaaatcacg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
     8761 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
     8821 atagcttccc ctattttaca aaattgtgcc ttattgccga taggctatct cttgaacacc
     8881 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
     8941 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
     9001 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
     9061 gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac agggacttta
     9121 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
     9181 ctgagcttag ttgcttttgg aagtccaatc attttataca ttttattagc tttgattatc
     9241 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
     9301 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
     9361 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
     9421 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
     9481 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
     9541 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
     9601 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
     9661 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agtgaacata
     9721 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
     9781 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
     9841 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
     9901 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
     9961 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    10021 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    10081 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    10141 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccggctcc agaagaaatg
    10201 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    10261 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    10321 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    10381 aaaagtgaaa cagcgttgga gttgatcaaa cgcggtcatc gattgatcgc agacgatcga
    10441 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    10501 catctaattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    10561 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    10621 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    10681 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    10741 gcgatgaatt tccgtgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    10801 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    10861 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    10921 tcatcgtatc aggtatcatt atcgccatgt ggcttagcag cagagaggct gttcgggtag
    10981 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    11041 gtgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    11101 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    11161 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    11221 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    11281 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    11341 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    11401 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    11461 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    11521 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    11581 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    11641 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    11701 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    11761 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    11821 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    11881 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    11941 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    12001 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    12061 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    12121 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcgaaaca tgacatcact
    12181 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    12241 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    12301 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    12361 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    12421 ggtgccaacc cgctgacctt tatcggatta agcggagtag gcgatctagt cgttacttgc
    12481 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    12541 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac tcaagcagcg
    12601 atggaactag cccaaataat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    12661 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatgggaaa
    12721 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagtcaaaa
    12781 aagcagttat tccagcggca ggattaggaa caagattttt accagcaaca aaagctatgg
    12841 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    12901 tgaaatcagg aatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    12961 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    13021 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    13081 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccgtttg
    13141 tcgttatgct aggcgatgat atcatggaag atgaagtacc attgacacag caattaatcg
    13201 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    13261 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    13321 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    13381 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    13441 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    13501 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    13561 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtat atcctgtctt
    13621 taggtaaaaa attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    13681 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    13741 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    13801 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    13861 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    13921 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    13981 acaaaagacg tagatctttt gtctagacaa gtagaaggct tgctggtcaa aagtaatgaa
    14041 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    14101 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    14161 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    14221 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    14281 aaaaaggtgg atttttatta ggtgcagtag tcggaggaac agccgcagca atcgccgcgt
    14341 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    14401 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    14461 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    14521 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    14581 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    14641 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    14701 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    14761 aagcaaaacc agttgcagat gacacaaaag aaatgatgaa aatggcaaaa gaagatgcga
    14821 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    14881 actaaaatca caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta
    14941 ctcttatttt tctaaaacta gtaattcttt tggtgtagtc gtgaatggca cacagccatc
    15001 gcttgtcaca tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc
    15061 tggttcaaga gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga
    15121 agggaattcg tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc
    15181 gtagccatag ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc
    15241 tggacggacg gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt atattttttc
    15301 ttggaagtcg gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt
    15361 ccaaacaacg ccaagatcga ataacacaag ttcattcggt gaaatcttcg tgtttcctgg
    15421 tgtaccatga gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg
    15481 tgaaacacct tgttttttca attggtattc gatttctgca atgatcgctt gttcagtcac
    15541 gccttctttc actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc
    15601 gatcagtttg tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagc
    15661 tagatcagtg taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac
    15721 agtcaaggcg ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc
    15781 ttttccgatg atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca
    15841 actactgttt tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc
    15901 aagagggata aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta
    15961 agcgatgtgg ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg
    16021 acgtagttca ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt
    16081 attcaataat tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta
    16141 aaaattttca tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta
    16201 ttttagaaat gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa
    16261 tggaaaagca aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta
    16321 ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct
    16381 tggaagttat cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta
    16441 agaaaacaac aacagttggg gtaatcatcc ctgatgtcag caatatgttc tttgcttctt
    16501 tagcacgtgg tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt
    16561 cagatggtaa cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg
    16621 acggcgtgat cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt
    16681 caaaaactcc tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa
    16741 acattgatta tgtatcagca acaaaagatg cagtaaacaa attggcaaaa agcggcaata
    16801 aaaaaatcgc ttttgtcagt ggtgcgttga tcgatcctat caatggacaa aatcgtctga
    16861 aaggctataa agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg
    16921 aagcacaata caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg
    16981 caacagctgt ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag
    17041 accgtggcgt gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga
    17101 cagaagtagc tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg
    17161 tatctatgcg cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta
    17221 tcttgcctta tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag
    17281 aggttgtgag cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcattt
    17341 catgtttgat gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttattcg
    17401 ggaaaagggg ttatgacaaa attttgttta atcctttttt tttgtcttaa aaaagagaga
    17461 aagtggttga aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattatg
    17521 ttcaccacca tcaaaaaaat aagggactgt gtattctatt aaatcaatca tctttctcat
    17581 ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc cagtaatctg
    17641 tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc ggtccatttt
    17701 tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta ttgacagtaa
    17761 cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca tcagatgata
    17821 attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct tgattgatca
    17881 gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca gtattgtcat
    17941 cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc catgaatctc
    18001 ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga tttccgttta
    18061 agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca ctgcgcatca
    18121 tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg ctttcgtgtt
    18181 tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct tcttcataga
    18241 cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg acatctactg
    18301 tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc atctttgcca
    18361 agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct tgatataagt
    18421 gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg aaggtgttcg
    18481 atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct tgccaagttg
    18541 ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt gcaggtccat
    18601 aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag gcatggttat
    18661 tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca gttctgttat
    18721 ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac atgtatccat
    18781 aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc gaatggattg
    18841 tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca ctgaccgatg
    18901 aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt cttgttgctt
    18961 cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt tgttgtggta
    19021 gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc gtaatttggc
    19081 cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca gaaagatctt
    19141 cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg ggtaatccag
    19201 cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag attcctcttg
    19261 cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat ggtgagacat
    19321 tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt gcagaaagaa
    19381 tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc tgtttgacta
    19441 attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca gatactaatg
    19501 ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca tcctctgtgc
    19561 tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc gtgcgcatca
    19621 gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt gacgtttctg
    19681 taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg tcttcgacga
    19741 gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc aaaagaagaa
    19801 cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga acaaaccatt
    19861 tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa agggtcacct
    19921 caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa cagacaaggc
    19981 tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg atctttttta
    20041 taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag aacagggcaa
    20101 agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat tgttagtaac
    20161 aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag catgagcgga
    20221 aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc gacttttatg
    20281 atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat gaatattgat
    20341 gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca tgccccttat
    20401 atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt ccaatttctg
    20461 agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact tcacccaggt
    20521 gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg cttgaatgaa
    20581 gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg taaaggtacg
    20641 gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa attgaatgaa
    20701 aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa tattaaagaa
    20761 gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg gcttaaagta
    20821 ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca cgccaatatt
    20881 ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc aatgtttgac
    20941 aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa agcttacgca
    21001 ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga agtgtttcct
    21061 gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag caaaaaagga
    21121 ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt ttgcagtctt
    21181 tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt atactggtag
    21241 aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg aaggacgaaa
    21301 agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat acatacttta
    21361 ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt aaagcaatct
    21421 atggaacaga catgtcggca acctatgatg accagctgct gattcgctat gctcaaaata
    21481 ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa ggaatctcct
    21541 acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt ttgttcggca
    21601 tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc gtgaagaaca
    21661 agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt tttacacatg
    21721 aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag atcattatcg
    21781 cttgtttact agctatttat taccgtaaat atagttccag caaaaaaatg ctgccgtggt
    21841 ttttagggct agctgtttct tttcctttct tttggttcat tcgcgaagat tccatctggc
    21901 ttcttccttt tactgtagtc gcgttaggca tcagtttcgt ccagatcttg ctaggacaag
    21961 acttttcttt caaagaagca aagaagttaa aagaacgacc atttctcctt tcgaaacaag
    22021 aatggctgaa actttcattg tttttactcc ctctagtcgg tttctccatc gtctatcagt
    22081 ggatcgaata taaaaacgag acaacatata gtattgccgc catcaatgac cgttcgagtg
    22141 gcgaatttgg acgtttgatg aaacagctga tccgaataga tgacgaaacg aacttaaata
    22201 aagaaaacag tgagatatgg gtctcgcatg ctgcattaaa taaagcatta gacgcatctc
    22261 ctacatttca tgctgtttct actcgtattg acgaattgta tacgactcat gcgtggaccc
    22321 aaggtggaaa agtaaaagaa ttaccaggag atatcatttt ttgggcattg cgagatgtgg
    22381 taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt tggaaaaaag
    22441 tcaatcaaga attagaagaa gcctatcaaa agggaactat caaaaagaaa aaagaattct
    22501 atctgatggg atcaggggat ggaaagacga tgaatgatct gccaatcgta tttgcttttc
    22561 tgaaatcagg atatgcctat gcatggcgat acaaagattt ccatcaaggg gggaattata
    22621 gtttcggtac gcaagaacaa gtcgaacagg cccaagaatt attgaatctt ccactaatgg
    22681 ataattggat cgatagcagt gatccgagaa cacctgattt ccagttgaca aaaacagcaa
    22741 aagtcgcgaa tgtcatgata aaaatttacc aagttctggc cattcccgtg ctgattgctt
    22801 caatagcagg gatattactt atgatactag gaaccgcatt tgcaaaagct gatcgtgcct
    22861 attatagttc ctctttgatt gtagtcaccg gattaatact gacacaattg gtttttcttt
    22921 tcggcgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt ttaagtgtct
    22981 acactggcgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg ctagttttca
    23041 gtaaattgaa tctgaaaaaa gctttatctt ccaaataaaa acatactaag attagaggag
    23101 aacatcattg acgaatgatg ttctcctcta ttttctattt ttagattata gtgaagagtg
    23161 aaagaaagtt cgaacaaagc cttgaggact atgagtccct atcaaaaacc gaacttcttt
    23221 actgtcattt tagaatcagg tttacgtatg ttggggccat gagcccgtgt ataggaaatt
    23281 ggaatcgaaa tgaagcagta aagttctttc aaaacttaaa aaggaggaaa acaaatgcct
    23341 tacattcttg cattagatgt ttcaatggga cacagctaca gtgtcctata caaagacgac
    23401 acctgcttat ttgaagacga gatcgaacac actcaaagag gatttcatgc attactcgaa
    23461 gagatccagc acttgcctga acgtccattg attgtttttg aagcaacggg gatttattcc
    23521 agaccagtcg aaaagttctt tcaagatcat cacttttctt actgtttgtt aaacccgtta
    23581 gaagcgaaaa aacagatgga agaaaacacc ttatgcagtt ggaaaacaga taaatcggat
    23641 gcccatcggc tcgcacagac tcatcttaaa aatcaacgtc agccaaaaga agtacaaagc
    23701 aatttttact tagagatgcg cgatttagcc cgtttttatc aagaaattga gaagaaaatc
    23761 actcgtctgc gcatggattt acacaactgc cttcagttga cttttccaga gcttgaacag
    23821 ttctttttaa acaggctgac cccttacgca ttgatattga tccgtctttt tccccatcca
    23881 gattttgtat tggcatctac tcggacaaaa ataaaaaata aactgatcaa cgaaacacgg
    23941 aaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca ctatgcacag
    24001 tgtgcctatc cagctgttga aaaagacagt atccactgtc aaaaaacgat ctattacgct
    24061 gaacttctcc aagatttact cgaacaaaaa gaagcactgg cgacacaaat gatcaaaaaa
    24121 gccgaaggct ctccttgctt tttgttgtac caaacgttcc ctgggattgg cgcactcaca
    24181 gccgctttat tgctcggtga attaggagat atcacacgat ttaaaaccca taaacaattg
    24241 aatgcctttg tcggaatcga catccgacgg tatcattcag gtaagtatac tggccaagac
    24301 cgcatcaata agcgtggtaa tcccaaagca cggaaaatta ttttcttcac cattcgaaat
    24361 atgattcgcc agcaacgcgc agctcctaat catattgtcg attattacta caaattaaaa
    24421 aagcaaccta tccctaagaa agataaggtt gccacagttg cttgtatgaa caagcttctg
    24481 aaatgtatgc acgctatggt tagggcacat acagagtacg actatgcgta cgcggtctca
    24541 gtggaccatt aactaaacca cttacagtat accacttctt tgcccagatc gaaaattttt
    24601 tacgaacggg gctttatttg gtgtgccttg attttgtcgc tttcgtttat gagctatttt
    24661 tcttacacct tttacttgac taatcgtagg aaagggaact gtgacaaaag tcttgtcgca
    24721 gttccttttt ccgaataaac ggtgctcaaa agcttgcatc tgcggtaata agtccaacca
    24781 agttaaaaac ataaaaagct gtttttactt ggctgttctt attacttgat gcaaggcagc
    24841 tttttcacaa cctctgatac acggtgttcc agaagtaact tcttcggaaa taagccgaaa
    24901 tttcacaaaa atttgaaaag caattttctt aaattccttc ttatttcttg aagctgccca
    24961 cttctgacac aacctccttt gtgtaaaaga atcagtttct actcgatttc ttctatcgtt
    25021 tcctgcaaac gctgttcata ttttttgttt cctttatata aatcaccaat cgtccatgcc
    25081 aacaggagaa ggacaagaac aatgattaca taagcgattt gatcagccaa tgctagttga
    25141 gctgaggtgg cttgctcgcc gaagaaactt tcgatttgac ctggtaatcc tcgtaaattc
    25201 aagtaagtca gactaatcac agagaaccag ccaagtagtt ttacaacaaa tgaatttttg
    25261 aatcggctcc ccatctccgc ttggctattg gtcatcatca gtaaaggaat catcgagaat
    25321 ggcaatgcaa aagccagaaa aacttgggat tcattcatca agttattcaa agcggtatgt
    25381 tcttcgatcg ttccttttgt acttgtaaac aaaacacaaa tcaatacagg aatcacagaa
    25441 agcaatcgtg tgactagtct tcttagccaa attgggatac gcatatggat gaagccttcc
    25501 atgatgactt gaccggtcaa cgtaccagta atagtagagt tctggccaga agcaagaagt
    25561 gctacagcaa ataaagtaga caagatacca gattttgcta cagcaatcaa gataccattg
    25621 ctcatagctg atggatcaga taaagcttca tacagaccaa aaaaggaagg atcttggaca
    25681 gaaccggttt tgaatacagc cacacccatg atcaatagca gtgcattgac tagaaaagcc
    25741 attgacaact ggatattaga atcccaagta gaaaagcgta ccgcttgtgc tacatcttct
    25801 tcgtcttggt gatcgatctt ccgagtttga gaaactgctg aatgaagata taagttatgc
    25861 ggcatgactg ttgcaccaat aatccctaaa gctccagtca gcggagtttc gccgccgatg
    25921 accggtgagt ctgagaatgt gtgactgtta gggataaatc cggcaaacaa actgtgccaa
    25981 tcagggttag acaagccaac ttgatagacg aaaacgaata aaatgacgaa aatcaagcag
    26041 acaaccaaag cttcgatttt acgaaacccg acttttgtca gcaataaaag gacgaaaaca
    26101 tctaacacag tgataaatac ggcaattacc aatggaatat caaaaagcag gtagagggcg
    26161 attgctgcac caatgacttc tgcgatgtct gttgccataa tggctaattc agtcaagatc
    26221 cataaaatga cacccaatgt cttacttgtc cgtgctcgga ttgcttgggc caaatccatt
    26281 tgggtcacga ttcctaattt tgctgccata tattgtaaaa gcatagcaat cagactagac
    26341 atcaaaataa ctgacatcaa taaatattgg aaattttgtc caccagtgat cgaagtggac
    26401 cagttacctg gatccatata accaactgct actaatgcac ctggtccaga gtaagcaaga
    26461 agtgttttaa aaaaactttt tcccttgggt acatccactg ttccatttac ttcagctaaa
    26521 gataaaccat ttgtatgttc aataagtcga tgcttatgaa attgtttgtt ggattgatta
    26581 ttcaagaaaa aaccaccttt cctatttatc tacgattaca ttctacacct cagaaagaaa
    26641 aaattaaaga aaaagatttg gtatgcctaa ttttttttcg tggcgtgtct tgctggttaa
    26701 tgaaagaaat tttgttcttc tctaaaaaaa ccactatttt ttgcatgaaa ctaaagaata
    26761 cgcctttctt ttttcaataa actttagtaa aataaatcat tgagagcaat tcggaaatag
    26821 aaaggagaga tttatatggt atcaacaggt attgtcgtac ttattgctat tattgctttg
    26881 ctaatcggcg cagtaggtgg ctttttccta gcgcgtaaat atatgaaaga ctatttagaa
    26941 aaaaatcctc ccgttaatga ggacatgttg cgatcaatga tgatgtcaat ggggcaaaaa
    27001 ccatctgaga agaaaattcg ccagatgatg cagcaaatga agaaccaagg gaaaaaatag
    27061 gttctgaccc aaagactttt tgcgggctga ttttttcagt ccgtttttta tttcgtaatt
    27121 tgttagacta ttcagactat tcagcaaaaa agacaagcac agacaatcct atgctcttga
    27181 taaagacaaa ggaggagtaa aatgtcgatt tttagaaaat taggttggtt ttttcgacag
    27241 gaaaagaaac attatttaat tgggattttc tccctattcg cagtggcctt ggtacagtta
    27301 gctcctccaa aagtgattgg gatcatcatt gatgagatcg ccgataaaaa tttatcaata
    27361 aagatcattt cctggtggat cttattattg atccttgcag ctctcttaca atatcttttt
    27421 cgttatatct ggcggacgaa tatttgggga agtgcagctc ggttggagaa agaacttcgg
    27481 cagcagctat tcaaacattt tacagaaatg gacaatctct tttaccaaaa atatcggact
    27541 ggagatttga tggctcatgc aacgaatgat ttgaatgcaa tccaaaatgt agcaggagct
    27601 ggtatcttga cctttgcaga ttcagtgatc acgggcggaa caacgatcat tgcgatgatt
    27661 ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt actagccgtt
    27721 acttcacgag tgctggggtc aaaactccac gatgcttttc gtgattcgca agaagctttt
    27781 tctagtatca atgataagac acaagaaagt attactggga tcaaagtcat taaaacattt
    27841 ggacaagaaa aagaagatct cgcggatttt gaagaaaaga ttgaagatgc aattattaaa
    27901 aacaaacgag tcaatttttt agactcctta tttgatccat tcattacctt gattatcggt
    27961 ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg cacgatctca
    28021 atcgggcaga tggtctcatt tattagctat atcggtatgc ttgtttggcc gatgtttgcg
    28081 attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg cgtcaatgaa
    28141 cttttgcatg aaaagacgca tatcgtggaa caactgggtg cagtgcagac tccggcaaaa
    28201 ggcgagctga atgtagctat ttcctctttt aactatccga aagaagaatc accagcatta
    28261 gaacaaatca aatttgcgat ccaagaagga gaaacattag gtattgttgg gaaaactggt
    28321 gcgggaaaaa cgacaatcat caaattactg atgcgtgaat acgatgatta tcaaggaaaa
    28381 attcaatttg gcggatgttc gatcaaagaa tattcattga atgctttatt aggatcaatc
    28441 ggctatgtgc ctcaagatca cttcttgttt tcgatgacca ttcgagataa tatccgtttt
    28501 tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc atttatcaac
    28561 aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg tggagtctct
    28621 ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa aaatcctgaa
    28681 ttgctgatct tagatgatgc actttctgca gtagatgcca aaacagaaga agctatcttg
    28741 cataatttaa aagaaataag aaaagagaaa acaaccatca ttgcagctca ccgtttaagc
    28801 agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat tgaacgagga
    28861 acccatcatg agctgcttgc attaggcggc tggtatgaac gaatgtggga gaaacagaaa
    28921 ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa tcggaatgga
    28981 caaaatcgat tccacttaaa gaacagaaga agatcatcaa acgactgatg aagttcgcca
    29041 aaccttttag aagaacgttc atcgttgcta tcttatttgc gtttgccctg tctgtgatca
    29101 atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct aaacagtcgg
    29161 ctacgacaca agttattttg ttttttgctg gtatgtactt gtttggagtc atagtcaaaa
    29221 gtatcatttg gtttttccaa tggtatttgt attccatggc ttcattgaag acctatcagt
    29281 atgttcgggt aagattattt gaaaagctgc atacactagg tatgcgttat tttgatcaga
    29341 caccggcagg atcgactgtt tctcgagtga caaatgacac agaaacgcta tttgaatttt
    29401 ggtatgtctt tttgatggta ttgactggga tctttgcagt cgtttcttcc ttcattgcca
    29461 tgtttcaaat caatggagaa attgccttgt ggactttggt gttcttgccg attttaggaa
    29521 tcgtcatctg gtattaccag aaattcagtt ctcgtattta ccggaatatg cgggaaaaat
    29581 taagtcagct gaacacgaaa ttgaatgaat cgatttccgg catgcgtatt atccagcagt
    29641 ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaacgag tatttagaca
    29701 cgcgctatgc gatgatcaaa acaaattcga ttctgttaag tccgatcatc aattttctat
    29761 atgcattggc gattgcgttg gcgttgactt tgtttgggat cgatgcgctg cattctcccg
    29821 tagaagttgg actgatctat gcatttacga cctatgtcca gggatttttt aatccaatgt
    29881 ctcaaatgat ggactttttg agcaccttta cagatggaat ggttgccggt agtcgtattt
    29941 tgaaaatcat ggatacagaa gaatatgcac cgcaacaaaa tcctggagca gatcaagcga
    30001 ttacttcagg taagatcgag tttcgcaatg taagcttttc ttatgatgga gagcacaatg
    30061 tattgaacaa tatctctttt gtcgctaatc cgggagagac cgttgcatta gtggggcaca
    30121 ccggaagcgg aaaaagctcg atcatcaacg tattgatgcg gttttacgaa ttttatgaag
    30181 gccagatctt gattgatgat catgacattc gagaatatcc aatggaagag ttacgtaaga
    30241 aaatgggatt ggtcttgcaa gatgccttct tgttttatgg agatatcgca ggaaatatcc
    30301 ggatgatgaa tccaactatc acagacgaac agattcgcca tgcagctgag tttgttcaag
    30361 caaacaagtt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc gaacacggtg
    30421 ccagctattc tagcggacaa cgacagctga tcacttttgc gcgaacaatc gtgacagatc
    30481 ctaaaatact agtattggat gaagcgacag caaatatcga tactgaaaca gaaggattga
    30541 tccaagaagg attgacaaaa atgcgacaag gacggacaac gattgcgatt gcccatcggc
    30601 tgtccacgat tcgggatgca gaattgattt tagttttaga caaaggtcat atagtagaac
    30661 gtgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg tacaaactgc
    30721 aaagcagtgg tgtgtaaaaa gtggttgtga cagggctccg ttcagcttta ggtgataaga
    30781 aaaaaattgt aaaaaaggct ttccagattt ttgcataaat ttggcttatc accgataagc
    30841 tggtactgga acaccgtata ttagaagttg ggacagtagc atagtagcat tttgacctaa
    30901 gcgctagagt gaaaataaaa cagagcttcc cagattcttc atctgtgagc gttagtgtcg
    30961 taattaccag atttaaaaaa aatcacgaca gctagtcaga agtgcccaat agccgctaag
    31021 aaagcgttga caaaagtgct tgctttcgat aatataaaga aaacgataca acttggatag
    31081 tgattgcctg atgcaagcta aacctgatga gtctcccatg gttccatgga cggctttttg
    31141 ggtttttctt tttgttttgg atacagccct atatatcatg gaaaggagta aaacatgctg
    31201 attgagaaag tactgaataa caacgtcgta ataaccaggg atgaaagaaa tcaggaaatg
    31261 atcgtgatgg gaaaaggttt ggcattcaaa aagaaagtgg gtgacccgat ttttgactca
    31321 tgcatcgaca aaaaatttca gttgacaaat gacggacttt cacaacagtt ccaagaattg
    31381 gcacaagcga ttcctcttga gtacttagaa atttcttgtg aaatcattga atatacaaaa
    31441 gaggctctac aagtccagtt gaatgattcg gtgtatattg cattgacgga tcatttgtat
    31501 acggctatcg aacgtcacaa aatgggaata tttgtaccaa atgtattgct gtatgatatc
    31561 aaacaactat ttcctaaaga gtatgccatc gggaaaaaaa cagtgaagaa aatctcggat
    31621 aaataccaca tcaagttatc agaagatgaa gccggattta tcgcattgca tttagtcaat
    31681 gcacaaggca ttcaaagcca acaagacaat acctacctgt ttactacaac aatacaggat
    31741 attctaaata tcgtccgcta ttactataag attacatttg atgaagattc agtccatttt
    31801 tttcgattcg tcacacacct acgttttttt ctgacacgcg tcctagatgg agaaaaatca
    31861 gaagtgggag aagtttctga ggaattgtac caggttattc aaaaaaaata taaggatacg
    31921 tttggttgcg ttgagaaaat agcagacttt ttagccgaga tattcaacta ccagatgtcc
    31981 acggacgaaa aaatgtattt aacgatccat attgcgcgtt tagtgcatga gaatcgttgt
    32041 aaataaataa aaaaaggagg ataaattaac aagaacgcat gaaataaggg tagtgacatt
    32101 cagttggcta aaccttccac taataaattg gagggaatag aaatgggaaa atatcaagaa
    32161 ttagcgagca aaattgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat
    32221 tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg
    32281 aaaaatatgg acggtgttgt gacggtaatg cgagcgggag gacagtacca agttgtcatc
    32341 gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat
    32401 gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc
    32461 tgtttccagc cattccttgc tattctagct gctggtggga tgattcgtgg actgacagca
    32521 ttacttgtct tcttaggcgc atttgaccgc ggttctggca catttgttat gtttgataat
    32581 atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa
    32641 tttaaagtaa atgaattcgt agggatgttg cttggggcag cattaatgaa tcctagcttg
    32701 tcgctggaag ctttgagtgg tgcagcagaa gcaccactga ctaccatatt ttctggcact
    32761 atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac
    32821 gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca
    32881 attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt
    32941 attacaatgc ccttaggttt tttactcgtt ggaccagcat tcacattcgc aactgatatt
    33001 ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta
    33061 ggcttcttct ggcaaattct agtgatgttt ggtctccatt gggcaatcgt accaatggga
    33121 ttgatgcaat tttctgtgaa tggttggcaa aatatcatga cacctgttgc agtagtgtct
    33181 tttggacaaa ctgcagcact tactgcactc tactttaagc ttagaaaccc aaaagacaaa
    33241 gcaatcgcaa taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat
    33301 gggtttacat tgccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg
    33361 ggcgcgtata gtggattaat gaatttaact tcttggaatc caggtggatt aggcatcttt
    33421 acaattccca actatattag accagatggt gatttaacgg atgtgattaa tgtattgatt
    33481 ggaatcgcca ttgcgatggt ggtatccttt gcgctgactt ttttcttttg gaaggaggaa
    33541 gcaggtgaaa cagatgatag acagaaaaga tcaggtaaag aaattgtaaa aacaccgata
    33601 caaggacaaa tcgctccact gaatgcagca aaggatgcag cttttgcaca aggaacacta
    33661 ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca
    33721 gtcatgaccc tgttcccgac gaaacatgca atcggaatgg tttctgaagc aggtctggaa
    33781 ttgctgattc atgtcggatt ggatactgtt cagttggaag ggaaatattt tgaaagcttg
    33841 gtgcagcaag gagaaagagt gaaacaaggc gatctgttgc tccgcttcga caaaaagaaa
    33901 atcgaagagg aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat
    33961 ttggatattt tggaaaatca aggggagacg gtcgggccag acgatgcact tatcaccgca
    34021 ctgacctgaa cggttaggtg aaagaatgga ctaggcttaa ttattctttt tagtccactt
    34081 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcaa
    34141 atttttgtcc tacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    34201 aactatataa cacgtctaga agataacaaa tcgatgtatt gatcgtaaaa ttaccaattt
    34261 tcgaatacag tcgttcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gaagatagcg
    34321 ttgaatcacc gatactggtc aaagctaaaa ttggaacatg attttgtttc aatatttctg
    34381 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    34441 tcgtgcaatt gtacgcttca tagaacgatt caccaaaagt ggtgacaatc gtgacatcca
    34501 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    34561 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    34621 gatgaatgag agaaagtgta tcttgaatcg tcatctgttc taaagaagcc attttatttg
    34681 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    34741 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    34801 aacccatttt ttttgctaca cggatgagtg tggacgggtg gacataacat cgctcagcaa
    34861 tctcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tgaatcactt
    34921 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    34981 cttcttcttg tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    35041 atgttatata gcttaataaa gacatttttt gacaagatat ttacaaaaat ccagtagaaa
    35101 acactaactt ttgtaaatgt tactggaaag aaacgaattt ctattgtttg ttttgtttac
    35161 aaaaaagata ataaaagtgt aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa
    35221 tcgctacgat tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa
    35281 gatatgagga attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa
    35341 agcttgagat cgtcagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca
    35401 aaatctatgc aactctggat cgggaagaag cattgaaaga tgcggatttt gttacgactc
    35461 agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg
    35521 ggatgattgg gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc
    35581 cagtggtttt ggcaatcgtt gaagacatga agcgtttatg tccaaatgct tggctgatca
    35641 atttcgcgaa cccaagcggg atggtgacag aagcagttat cagatatggc aaatgggata
    35701 aagtgatagg attatgcaat gtgccagtaa tggcgatgat gacagaacca acaatgcttg
    35761 gcaagaaaaa agaagagctg atctacaagt ttgccggtct aaatcacttc cattggcaca
    35821 aagtagcaga taataaagga aatgaccgca cggcagaatt gattgatcgt ttatacgatg
    35881 aagacaatgg attgccgaaa aatatctttg atatcccgtt tttcaaagaa caattaaaac
    35941 aaatgaacat gatcccttgt ggctaccacc gctattacta ccgtgaagaa gaaatgctgg
    36001 aacatgcttt agaagaatat caaacgattg gtacaagagc gcagcaagtg aaagaaacag
    36061 aagcagagct gtttgaatta tacaaagatc cagaattgga tcataaacct gaacaattgc
    36121 aaaaacgagg tggtgcttat tattcagatg ctgcctgtga aacgattgct tcgatctatg
    36181 gaaataaaga aacacagatc gtggtatcta caaagaatga tggagctgtt cctgatctgc
    36241 cggcagattg tgtagtagaa gtcactgcct atgttggcgc acaaggcgca cgaaatgtag
    36301 cgtttggttc ccttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat
    36361 tgctgacgat cgaagcagct gtgactggtg attatggaac agctttacaa gcgtttacga
    36421 tcaatccgct tatacgatca ggaaagacag caaaacggat catgaatgag ttgtttattg
    36481 cacatcagca gtatttaccg caattcaagg aaacaatcag ccggttaaaa gcagaaggaa
    36541 tcgaagtcca agatgaactg gcaagagaat tatcaagcaa tacacatgta atggaggaag
    36601 tatgatggga ttcagaaaag attttttatg gggcggagct acagcagcca atcaatgcga
    36661 gggcggttat gatgaaggcg gacgtgggtt agcgaacgta gatgttgtgc caatcggaga
    36721 agaccgtttt cctgtcatta ctggaaaaaa gaaaatgttt gaatttgatg aaaatcattt
    36781 ttatccagca aaaattgcga tcgatatgta tcatcattat aaggaagata ttgctttatt
    36841 tgcagaaatg gggttcaaga cttatcggat gtccattgct tggagtcgta tttttccaat
    36901 gggcgatgaa aaagagccaa atgaatctgg tttgaaattt tatgaggatg tctttaaaga
    36961 atgtcaaaag tacgggatcg agccgcttgt gacgattaca cattttgatt gtccgatgca
    37021 tttgatcaaa gaatatggtg cgtggcgttc cagagaactc gttggttttt atgaaaatct
    37081 gtgccgagtc atcttcaatc gctataaagg acttgtcaaa tattggttga cattcaatga
    37141 gatcaatatg atccttcatg caccattcat gggcgcaggg ctgtattttg aagaaggaga
    37201 aaatgaagaa caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc
    37261 aacgaagatt gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg
    37321 agcatattat ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg
    37381 agaaaattat ttcttcattg atgtccagtc acgaggagaa tatcctgctt atgcattgaa
    37441 agaaatggaa cgaaatggca ttcagatcaa aatggaacct ggggatgccg cattgctgaa
    37501 agaacatact gttgatttta tctccttttc ttattattct tctcgagttt cgactactga
    37561 caacgaactc ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct
    37621 ggaagcaagt gaatggggtt ggcaaattga tccattaggc ttgagaataa cgatgaatga
    37681 tatttatgat cgttatcaaa aaccactatt tattgtagaa aacggcttag gtgcagtcga
    37741 tacgcccgat gagaatggtt atgtggaaga tgactatcga atcgactatc tggcagcaca
    37801 tatccaggca atgaaagatg cagtagaatt agatggagtc gatctattag gctatacgac
    37861 ttggggatgt attgatttag tatcagctgg tacaggtgag atgaagaaaa ggtacggatt
    37921 catttatgtc gatcgtgaca acgaaggaaa tggcacatta gcccgttcga agaagaaatc
    37981 attcaactgg tataaaaaag taattgattc aaatggagaa aatctttcga atgattgatc
    38041 aatgatttat aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg
    38101 agaaacgcaa gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga
    38161 ttaagtaaag gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg
    38221 gatgactgct gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg
    38281 cgcccctggc ggccaaatga acaatacggc agaaatcgaa aactatcctg gtttcgattc
    38341 aataatgggt cctgatctag cttataagat gtatgaaggt gtttcaagat ttgggacaga
    38401 aaatgcttat ggaatcgtgc aagatatcaa ggatcacggc agccacaaag aagttatttg
    38461 tgaagataaa acttacgaag gaaaaacagt cattatcgca acaggctgta tccaccgtaa
    38521 attaggcgta cctggcgagg aagaatttgc tggacgtggc gtttcttact gtgcagtttg
    38581 tgatggtgcg tttttccgta acaaacgtct agtagtgatc ggaggaggag actcagcagt
    38641 agaagaagcc atctatttga cacaatttgc ttctgaagta gtcatcgtcc accgtagaga
    38701 tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt gcgaatgaaa aaatttcctt
    38761 tatgtgggat acagtcgttg acgaaatcgt tgggaatgaa atggtagtga caggtgtgaa
    38821 agcacgtaat gtcaaaaccg gcaatgagtt tgatttggca gcagacggag tattcatcta
    38881 tgttggactg gatccattaa ctgcaccatt tagagaagca gggttgacga acgaagctgg
    38941 ctggattcca acagacaacg aaatgaagac gaatatacca ggcgtttttg ccatcggaga
    39001 tgttcgtgaa aaagatttgc gacaaatcac tactgctgtt ggtgatggtg ggatcgctgg
    39061 acaacaagtg tataaatact tggaatcttt agaagaaaca actgcataag tttaaaaaga
    39121 gtggaagaac ggtttaattg ttcttttact cttttttttc gcgtgaaatc tactgaatcc
    39181 gatttatgcg aatacttgca accgtgcttg ataaaagaat cctttcttga tttaagcgtt
    39241 ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt
    39301 tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa
    39361 gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa
    39421 cttgaagatg ctttttatgc accgttggaa ttcggtactg caggcatgcg gggaatctta
    39481 gggcctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga aggacttgca
    39541 cgtttcatgg atacacaagg cgaagaaaca aaacgccgag gtgtcgctat cgcttatgac
    39601 tcacgccatc aatcaccaga atttgccatg gaagcagcca aaacattagc acatcacaat
    39661 atcccatctt atgtattcga aagtctacgt ccaacaccag agctttcttt tgcagtccgt
    39721 catttgaaga cgtttgcggg aatcatggta accgcatccc acaatccagc agcttacaac
    39781 ggatataaag tctacggaga agatggggga caaatgcctc ctgcagacgc agatgcattg
    39841 acgaaatatg tccgtgaagt cagcaatccg ttaaaagttg aagtattatc tgacgaagaa
    39901 gctgaacata gcggattgat cacgatcatc ggagaagaag tggatgccgc ttatctagaa
    39961 gaaatcaaag cagtaacgat tgatcatgaa ttggtggaaa caatggggaa agatttgaaa
    40021 cttgtgtaca caccacttca cggaacagga aaaatgttgg gcgaacgtgc gttgaaacaa
    40081 gctggattcg aacaattcgt actagttcca gaacaagctg tcgcagaccc agatttcact
    40141 acagtcaaat caccaaatcc agaagaacac tctgcttttg agtatgcgat acgacttggc
    40201 gagaaagaag gcgctgattt gctgattgca acagatcctg atgcagaccg tttaggtgca
    40261 gccgttcgtt tgccaaatgg ctcctatgaa gtattgaccg gaaaccaaat cggagcactg
    40321 tttgctcaat atattttgga agcacaccaa tcgcgaggca ctttacctga aaacgcagcg
    40381 gtattgaaat cgatcgtttc tagcgaatta cctacagcaa ttgcggaaca ctatggggct
    40441 accatgttca atgtattgac aggattcaaa ttcattgctg aaaaaattca acaatttgaa
    40501 gaagatcatt cccatacttt tatgttcgga ttcgaagaaa gttacggtta tttagttaaa
    40561 cctttcgttc gcgacaaaga cgcgatccaa gcattgctct tattagcaga agtagcagct
    40621 tactataaaa aacaaggcaa aacgttgtat gatggcttgc aaacgatttt tgaaacctat
    40681 ggctactacg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa
    40741 atctctgctt tgatggaaaa attccgtgaa gaagcaccaa cagaatttgc cggagcaaaa
    40801 gtcatccaaa cagaagactt caaagagttg acgagaacag accgtgaagg aaacgtagaa
    40861 aaactaacga caccgccatc tgacgtattg aagtatacgc tggaagaaaa tggctggatc
    40921 gctatccgtc cttcaggaac tgagccgaaa atcaaatttt atatcggtgt aaaaggaaac
    40981 tcttcagaag acgcagctga aaaagtagcc gcttatgaaa cgattatcca aaagatgaca
    41041 gaagaataaa aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt
    41101 ttcaagttgg aggtgaatgg attgccagac acgcaaaaaa caaaagtaga gaaagtcagc
    41161 caactattca aaatgttgag tgaccccact cgtctcaaaa ttctgctgta tctgaaagac
    41221 ggggaacaaa atgtgacagc aatcatccaa gcagtggaaa tggaacaatc tgctgtatcg
    41281 catcaactgc gtttattaag agaaaaccat gtcgtcaagt cccatcgtga gggaaaagcg
    41341 attttgtata gtcttgatga ttctcacgta ctggatattt tgaaccagac actcaaacat
    41401 gtggaacatc aataacgata ggtgcaaata gagacaaact ggactgtgtc aacgaatctt
    41461 cttcgctgac acagtccagt ttttttaaag aaaaattgtt agtctgtttc ttcggtggtt
    41521 tcagttccat aataactatg gtcttctagg tctaaagcat tcaaaataag cgaagctgtt
    41581 tcttcaatag ataaagaagc gacattgatt actacgcagc ccaatttttc ataaagatca
    41641 ttcgcaaaat cgagttcagc tcgtattttt tcgatatcgg aataagcagt atcaggattt
    41701 aatccgtaag agcgcatccg ttctttacgg atattgttta aaatatctgg gttattcgtc
    41761 agtcccacga tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg
    41821 atcaaaggta gattggcaac ttttaaattt ttgttagcta aaaacaagct caaaggcgtt
    41881 ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctagaaaccc acgaggatct
    41941 ttcccgtcat cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca
    42001 ttcaataaat gaagggcgcc aggttgccga atgggcttga taccagttaa gcgctcgatt
    42061 tctgatatcg gcggagtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa
    42121 cgcgtcatct cgatcaattc ttgattgatc aatgtgtgca ggacaagagc tttttcttct
    42181 agtgcatctt gcagtgcctg catcaatgtt ttctcatctg tcacaaatgt tctgcggaat
    42241 aaattgaact caacgctagg atattgggcc atagtggctt gcgctagttt tgtcgcagtt
    42301 tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg
    42361 gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga atgtgtactt
    42421 cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacgttgat cgaacaatca
    42481 ctagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact
    42541 tatttagtga agcgcaatcg ctacgtatcc gctaaagaag tttttgacta tatgaatcag
    42601 gaattcaaag gagtaagtta cgatacgatt tatcgaaatc tccatgattt tgcagaaatc
    42661 gatttactgg aagaaaccga tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa
    42721 ggggaaatcg gccatcacca tcactttatc tgtacggtgt gcggaacaac gaaagaaatc
    42781 catatgtgtc caatgaatta tttcgaagaa cagctcggtg gatgtacgat cgaagggcat
    42841 agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt
    42901 tgataatttt aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat
    42961 ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag
    43021 ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg
    43081 atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta
    43141 aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa
    43201 aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag
    43261 attaaacgat gacatgaaaa cagcgatgaa agcgaaagat aaggaatctt tacaagtcat
    43321 ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga
    43381 agaagaagaa ttaacggtgt tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga
    43441 gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt
    43501 cgaaaaatac atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc
    43561 aatcacgcaa actggtgctt catcaatgaa agaatttggc aaagtcatgg gtgcgatcat
    43621 gcctaaagtc aaaggaaaag cagatggaaa ccaagtaaat gccattgtga aagaactatt
    43681 acaatcttaa ttttatcatt taaactgcgg acaatcattg tccgcagttt ttttgtgctc
    43741 gtaaaaaaga gatctaaaaa atagctagac gaaagaatag gattctttca tctagctgtt
    43801 tttttcctgt ttaatatttc ttatgttaaa ttgtgtttga tctggttatt cttctggtag
    43861 ttcatttgct tcttgtttat accctgatag gcgataggcg taatagaaga gtaagctgcc
    43921 agcataccca agtaacgttg ggaccatcca gccaagtccc aatgcagcaa aaggaacgtg
    43981 atgatgataa aattcgagca tgcttgctac aactctttgt tgactcacta aagcaggcga
    44041 atttgccagc atatccaata cggcgggaac agcagcaaac aacatcgtta cttgatagac
    44101 aaatggtgtc ttttggaaaa attttgctgt taaggaaagc aaaatcaacg caagcgacaa
    44161 tggatacaaa agcatcaata ctggtaaaga ccattggatg atattgtcca aaccagcatt
    44221 tgcaacgaca aaagagacga aagtcgttaa ccgcagccat gtcatataac tgactttagg
    44281 gaacagttta tggaaatctt gtgcaaaaga aacaacgagt cccatagcgg tagtgaagac
    44341 acctaatgtg accatcactc ctaagaagct agcgccgaag ttacctaaat aaaaattagt
    44401 gatctgagaa agtgcagttc cgccattttc tgataaagac atcttattca aactcatcgc
    44461 accgagaaga acaagaccaa aatagatcat gacttctagc gcaacgctca ggctaccagc
    44521 ttttgctgtt aattctgtga cttttttatc tttgaaacca aggtcacgga ctgcatggac
    44581 aaaagtgaca cttaaggcaa gcaaggctac tgcatcaacc gtgttataac cttctaggac
    44641 accatttgtt aaagcgtttg tttggtaagt atctgcagca gattgattta gatttcccat
    44701 tgggtggaat aaagaaacga caaaaatgat tgctaataat gcaagaaaca aggagtttag
    44761 atactttccg acgtatttga ccaaccgtgt gggatggaca gtaaggaaat aagcaagtaa
    44821 aaagaaaaga gcagagaata aaaacatacc taatgaatta tatttttcag gcaaaaatgg
    44881 tttagcagcc atttcaaaag cagtagcagc tgtccgtggt gtaccgaaaa atggtccgat
    44941 cgtcaaatga acaaggatta agaacaaggc agcataccat tttccgacag gtttagctaa
    45001 gtcatacaga ccatcacttt ttgtcactac tacagctaag atagctagta atgggaaaag
    45061 agcgcctgag atggcaaatc ctacgccagc actgacccag tttgcgccag ccatttgacc
    45121 taagtggaca ggaaagatta gattacctga cccgaaaaac atcccgaaga taagtgaact
    45181 gaccatcaat agttgtttcc atgaataagt ttttgtttct tccataaatt tttcctccaa
    45241 taattatcat tttgattgac gatataaacg ttgcagcaat tagtaatgcc actaatatca
    45301 gattccatga aaaccaccac cttaaaatat aaaaaaaagc ccttttccac ttgggaaaag
    45361 ggctctagat tctagaacgg taccaccttt tttcgcttaa aaaagcttct tcattcacgg
    45421 acgctttttg ataaagttat ccgctagcct gataatggag gcagccacaa tgatctacgt
    45481 tttcgacatt gactcggaaa tgatgttcgt ctattcgatt tattgtcatt tctcacttac
    45541 atgactcact gggaataaat cgttgaatag cctactgttt tcctcgacgc tttgctgtat
    45601 gtttatttgt tgacgtaata atacacctgt tttttttgaa atgcaagaat attttaacta
    45661 ttttgttttc tttttttgaa acaagaaaaa tacctagaga atacctctga atgaaacgga
    45721 atattttgtt ctccactttc atataactta cattctcaga ttctttttat caaaacgaga
    45781 cctttagcga atggaaacag ctatttttta acgtttcttg gtacgtatgg ttaaacgact
    45841 cttttacaac cttttcttat ttgctatata gtttcttctg agtatggtaa aataggaaag
    45901 aatgaatcga aaaaaagggg atgtctattt ttgacggaag agtctagttc tttagagatt
    45961 agattgacag ctgctgatga tgtcagcatg cttttaggtg cacacgacaa gcatataaaa
    46021 ctgatcgagg aaacaacgga aacaaccatc catacacgtg gagaaatcat tcagatcatt
    46081 ggtgaacaat cccagatttc cttggctgcc tcagtcattc ggacattaca ggaattgatc
    46141 aaacgaggaa tccacgtaag tacaccagat gtcgtgacgg cattgaaaat ggggcaaaaa
    46201 ggtacattag attattttgt tgagatgtat gaggaagaga tcgtcaaaga ccgtaatgga
    46261 aaacctatac gggtaaaaaa cagcggtcaa aagaaatacg ttgaatcggt agccaaacat
    46321 gacatcgtat ttggtgtagg gccagccgga acagggaaaa cgtttcttgc cgtagtatta
    46381 gcaattgctg ccctaaaaaa aggcgaagtg caaaaaatca ttttgacgcg gcccgctgta
    46441 gaggcagggg aaaacctagg ctttctaccc ggggatctga aagaaaaagt ggatccttat
    46501 ctgcggcctg tttatgatgc tttataccaa atttttggat tggatcatac gaaccgattg
    46561 atggaacgag gcgtaatcga aatcgcacca ttagcttaca tgcgggggcg gacattggat
    46621 gatgcattcg ttatcttgga cgaagcacaa aacacgacag tcgctcagat gaaaatgttc
    46681 ttgacccgtc tcggttatca atcaaaaatg attgtcaatg gggatacaag tcagatagac
    46741 ttgcctcgag ggacgacaag tggtcttgtt catgcagaac gaacactaaa gcaaatcaaa
    46801 aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa
    46861 atcatcaaag cttatgaaaa ggcggatctt catcaagaat aagctttgct taggaggata
    46921 agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa actcggaaag gcagcattgc
    46981 ctttccttct gttcttgttt tttatcattg tctgcggcgt tacatttagt agtgtcaaac
    47041 aaaaggccaa tgatttcaga gaaggccaag tagcagaaga gagtatccgt gcgaataaaa
    47101 cgatcgagaa cacggaagaa accgaacaaa gaagaaaact ggcagcagaa gcagtgactc
    47161 cagaatatac ttatcaaaaa gatctggcag atgaccagaa caatcggatc aagcaattat
    47221 tcgaattgat cgataagacg aatgatacca tcaacaaatc ttataatgac aaggtagata
    47281 aggcgaagga agatgaaaat atcccgaaac ctactacaga agagcggatt gctacactga
    47341 agaaaaattt tgaaaacgtg aatgcagaaa acgttgcgtt ttaccaaaaa ctgccgaaca
    47401 atttttattc gacgatattt gaaatgactg aaacggaaat tcacacggtt agggatgaaa
    47461 gtttgaagct cattgacgaa cagatgagta aacaagttcg tgaaagcgaa ttggaagctt
    47521 ttaaacaaga agcagaagat cagattcagt atttgaatgt cacaacagag cagcaacaaa
    47581 tgattcgcta cttagtggat caaggcattg tagtcaatga tgtattgaat gagaaaaaga
    47641 cagaagaatt aaaacaatct gcacgagaag ctgtacaacc agtgatgatc ttccaaggag
    47701 aaatcattgt tcgcgaagga aatcaaattg atgcttcagc gatgaaaaaa ttggaattac
    47761 taggattgac gaatcaaacg acttcgattt tcccactcat tgccatggtt ttagctgttt
    47821 tgcttcaaat cggcgtactg atttataatt cacttcaatt ccaaaatgca ggagaaagaa
    47881 caaaatacgt gcttttctat gtgacagcta tgtcagtcag catagtgctg atgaaatttt
    47941 tccaattatt ccaaacggaa caagctactt atatcccgct gttttatccc gctgcttttg
    48001 caccacttgt tttaagcttt ttcttgaacc gaagagcagg gatcatggct ggtattttcc
    48061 aagcagtatc tgcacttttc attttttatg attcgattgg cacgaacttt ctaacgatta
    48121 tcttgatgtc ttatgttttc tctgggctca tggcaacgat tgtcagacgc aaacggatct
    48181 cagaacaagg attatctgct gccatgtggg tcattatttt tcctgtgatg atggatgtca
    48241 ttttgatcgt ctatcaagga atgagctttg gtgatgcaac gacttggtta atgcttgtct
    48301 gcgggttcac tggtgcactc ttctcattct tgctgacgat cggtctacac ccatatattg
    48361 aattgatggt caatgatgac agtgtaattg tattaaatga actcagcaat ccaaatcatc
    48421 ctttattgaa acagttgctg gaagaagcgc caggtactta tcatcatagt atgatggtag
    48481 ctaatctaag tgctaacgct gtagcagaaa tcggcggacg ttctttgcta actcgagtag
    48541 cttgctatta tcatgatatc ggcaagatca aacatgctag ctttttcgtt gagaacttgc
    48601 cttcaggagc ggaaaacccg cacaatttct tattgccaga agacagtaaa catattatct
    48661 ttggacatgt gacagatgga gccaaaatat tggaagatta taatatgccg caaatggtga
    48721 tcgatatttg caaacagcat catggaacca cgctaatgcg ttatttctac atcaaagcga
    48781 aagaacgcaa tccggaagta acagaagagc aattccgtta tcctggacca aaaccgcaaa
    48841 gtcgtgaagc agggatcgtc aatattgctg acagttgtga ggctgcagta cgggcaatgg
    48901 atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt
    48961 ccgatggtca attagacgat agtggactga ccttgaaaga aatccgaaaa gtggaaaaat
    49021 cattgatcag cggtttaagt agcaccttcc attctcggat caaatatcct aaaatgaaaa
    49081 cagaagttga gaagataaag gaagaacaag aagaatagaa agaagcgatg tataatggat
    49141 attaccttta tggatgaaac aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac
    49201 ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg aaacagaaat gtctgttact
    49261 ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt accgtggaaa agatgcaccg
    49321 acagacgtca tcagttttgc tctggaagaa gaaggcgaag acgaaatccc agtgattttt
    49381 gaagatggag agaatccttt gcctcgtgta ctaggagaca tcatgatttc tattgaacgt
    49441 gctgctgaac aagcagaagc ttacggccat tcatatgatc gggaacttgg ctttttagcg
    49501 gtacatggat tcttgcatat caacgggtat gaccatatga caccagaaga tgagaaagaa
    49561 atgtttggtt tacagaaaga gatcttggat gcctatggac tcaaaagata aaaacgtaga
    49621 gaaaaacaaa catttcctga tgtctgtgga atttgctgtc caaggagtca aaaccgtttt
    49681 tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt atccttgctt tatttatggg
    49741 cgtgattttc cagcttgatc ggatagagtg gttatggctg cttttagctg tttttctcgt
    49801 ttggatagta gagatcatca atactgtttt tgaaaatgta gtggacatgt ttacggattt
    49861 tcattttcat ccgattggga aaaaaatcaa agatatggca gctggagcag ttttagtgac
    49921 cgcttgtttt gctgtcatca ttgggttgat tttatttgtc cctaaaatat atcatttgtt
    49981 gtttaattaa aaaggagaac gtatatgcca gaacataaat cagggtttgt cgcaatcgtc
    50041 ggccgcccga atgtaggaaa atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc
    50101 attatgagtg acaaggcaca gacaactaga aataaaatcc aaggagtata tactacacca
    50161 gaagctcagc tgatttttat cgatacacca ggaatccata aaccaaagca tcgtctagga
    50221 gattttatgg tagaagcagc ttacagtgcg ttgaaagaag tggatgcagt cttatttatg
    50281 atcagtgctg atcaaaaacg cggccgggga gacgatttta ttattgaacg tctaaaaaac
    50341 gtgcattctc cagtttatct gattatcaat aaaatcgata agatccatcc agacgagttg
    50401 ctgggaatca tcgaagatta ttcgacgcag atgccttttg cacaagtgat tccgatctct
    50461 gctacagaag gaaacaatgt cgaacgctta atggatgtat tagtcagtga aatgccagag
    50521 ggcccacaat attttccaga tgatcaagtg actgatcatc ctgaatactt tatcgtttct
    50581 gaattgatcc gtgagaaagt cttgttttta acacgagacg aagtgcctca ttctgtcgca
    50641 gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa tccatatcca ggccacgatc
    50701 atcgtcgaaa gagatagcca aaaaggaatc attatcggca aaggcggaaa aatgctgaag
    50761 caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt taggagacaa agtatttcta
    50821 gaactttggg tcaaagtcca aaaagactgg cgagacaaaa aagtctatct gcaagacttt
    50881 ggctaccgaa aagacgaata ctgatattct gaacttctta taggttaagt atagaaataa
    50941 actggataaa aatccgcacg ttgagaaaaa cggcggaaga tcgaaacata aataatctgg
    51001 atcggcagaa tctgcttttc cagatttatt ttttaggagg gagaatatga gccagcttgc
    51061 agaatcgaaa ggcttgattt tatctagccg taattacaaa gaaaaagata aattagtcaa
    51121 aatctttacg gaatcttctg gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa
    51181 tcagccatta gctccggctt tgctcccctt taccgaagca gtctatatcg gtaattttcg
    51241 aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc catgcgtttc ggaacatcca
    51301 agaagatatc tttttgagcg gttatggtac ctatttgcta aatctagtag atgcagcaat
    51361 cgaagatcat caatatgacc cgcatttatt ccaatttacg caacaagctc tgcagcggat
    51421 ggatcaagga gatgatgcag aaatcatcac gaatatcttt gaagttcaaa tactgcaacg
    51481 cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt
    51541 tgactattct tccaaatctg gcggtgtgat atgcgagaaa cactggccaa tggacgaaca
    51601 ccgttatcat gcagatcctc gcgctgttta tttcgttcga atgttttcgg ctatttctta
    51661 tgataagatc agtggaatcc acttgaaaga agaaacgaag caggcaatcc gtgacctcat
    51721 tgaccagttg tatgatgaat atgtcggtat tcatctaaaa agcaaaaagt tcattgatca
    51781 aatgaagtct tgggcacacg tattaaagac gccagagaaa aatatgcctg aaaaagagat
    51841 acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata
    51901 cagggacttc cgtctattat taaccgtaga ttaaatagat cgttcactga gaggatctat
    51961 tatataaaca agaaaactgc aaaatgaata cgtacattga ccaagatcag taaaaaacac
    52021 gtggtttagc gagttcggga gagtggaagc cggacccaaa cgatttttga agaggcactt
    52081 gcgagtacga taaatgaagc tgccgaatct ttattcggaa gtagggtgga accgcgaaat
    52141 tattcgtccc tatgtctgta atcagacata gggacttttt gtcgttttct ttcctgtatt
    52201 cttattgaca gcaacgctta aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc
    52261 gtccaagata tgattttaac attacaaaaa ttttggtcat caaacggctg catgctgatg
    52321 caagcttatg acacagaaaa aggagcaggt acgatgagcc catatacatt cttacgggca
    52381 atcggaccgg aaccatggaa tgctgcttat gtagagccat cccgccgacc agctgacggg
    52441 cgttatggag aaaacccaaa ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa
    52501 ccttcacctg aaaatatcca agagctttat ttagaaagct tgcgtctttt aggaattgat
    52561 ccattagaac acgatattcg ttttgttgaa gacaactggg agaacccatc gatggggtgt
    52621 gccggtgtgg gttgggaagt atggcttgat gggatggaga tcacacaatt cacgtacttc
    52681 cagcaagtcg gaggactacc ttgtaaacca gttacttctg agatcactta tggactagaa
    52741 cgtcttgctt catatattca agaagttgaa agtgtctatg atctggaatg ggctgatggt
    52801 gtgaaatatg gcgagatctt caaacaacca gaatatgaac attccaaata ttcttttgaa
    52861 gtaagtgacc aagacttgct actaagcaac tttgaccgtt ttgaaaaaga agctaaacgc
    52921 tgtatcgatg aaaatcttgt tcatccagcg tatgattata ttttgaaatg cagccataca
    52981 ttcaatttat tagatgctcg cggtgcagta tccgtaacag aacgagcagg ttatctttcc
    53041 cgtatccgca acatggctcg tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta
    53101 ggattccctt tattgaataa aaacacagca aatccagaaa acatgaagga ggctgaataa
    53161 gatggcaaaa gacttattac ttgaaatcgg gctggaagaa atgcctgctc atgttgtcac
    53221 accaagcatg aaacaattag aagaaaaaac agcaaaattt ttagatgaaa accaattgac
    53281 ttatgatacg atcgaaacgt tttctacacc gcgccgctta gcaatcaaag taacaaacat
    53341 tccagaacgt caagcagaca gcgaagaaga agtcaaagga ccagccaaaa aaattgcttt
    53401 ggacgcagat ggaaactgga cgaaagcagc tgaaggattt gtccgcggac aaggtctgac
    53461 gacagatgct atcactttta gagaattaaa aggcgtggaa tatgtctatg taacgaaata
    53521 cactcacggc aaagaagcaa aagaagtatt gacaggttta gcagatgtca ttaaaagcct
    53581 cactttccca gtaacgatgc actgggcaga atacgatttt gaatatattc gccctattca
    53641 ctggatcgtg gcattgttag atgacgaaat aatcccgttt gagatcttgg atgtcaaaac
    53701 agacagagct tcaagaggtc atcgtttctt aggcgaagat gtgacattta cgcatgcaaa
    53761 agaatacgaa gaaaaattga atgaacaatt tgttattgct tcaccgcaaa aaagaaaaga
    53821 aatgatcatc acacaagcaa acgaattagc cgaaaaaaat cactggatca ttgatctaga
    53881 tgaagattta ctagaagaag tcaacaattt agtcgaatat ccaactgtct tttctggcga
    53941 cttcgaagaa aaatatctat ctgttcctga agaagtacta gtcacttcta tgaaagaaca
    54001 tcaacgttat ttcgaagtac ggaacgatca aggtatgttg cttcctcatt ttatttctgc
    54061 tcgtaatggc gatcgggtaa aattggaaaa tgtggtacga ggcaatcaaa aagtattgac
    54121 ggctcgttta gaagatgcgg aattctttta taatgaagac aagaaagctt caatcgacca
    54181 atacgttgaa aaattaaaaa ctgttacttt ccatgaaaaa atcggtagca tctatgagaa
    54241 aatgcaacgg gtacaagtca tcgctcgcat cataggcgaa caagtaggac tatctgagga
    54301 agaattgatg gatctgcaac gcgctgctga aatctataaa tttgatctag taaccaacat
    54361 ggtaggagaa ttccctgaac tacaagggat catgggagaa aaatatgctc tgctgaaagg
    54421 cgagaaacca gccgtagcaa cagcgatccg tgaacattac atgcctatat ccagtgaagg
    54481 agatttacct gagactacag taggttctgt tttagcgatt gctgataaat tagatagtat
    54541 cttttctttc tttgcagttg ggatgatccc aagtggctca aacgatccat atgctttgcg
    54601 ccgtcaagca tatggtgtcg ttcgaatcat cgaatcgaaa aaatgggcct ttcctttgtc
    54661 tgttctgcaa gaaacgatta gtgaagttat cagtaaagat acggatcgtt ttggcattgg
    54721 cttgtcagcg ggacaacaac aggtgatcga ctttatcaaa ggccgtctgc gccaattatt
    54781 gacaacgaag aatatccgtc atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga
    54841 tctgacaaaa gtatttgcag cagcacaatt gttcaagcag catcttgcag atgaagactt
    54901 caaaccatcg atggaagctt tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga
    54961 aaaacaatca gaagtagacc ctgaactgtt cgaaaacgaa gcggagaaag aactgcacaa
    55021 agcagtagag gcaatgaaaa caagctttgc tgaccaaacg atcaacgaaa attatgatca
    55081 gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat cagacgatgg tcatggcaga
    55141 agaaccagca gtacgtaaga accgattaac gcaattaagt caaattgctg ccatggcttt
    55201 gtcactagca agtttagatc aaatcatcac aaaataattt ctaatatgta aataaaccag
    55261 agaacaggca caaaataggc tattt
//
LOCUS       DACCZY010000004        51938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCZY010000004 DACCZY010000000
VERSION     DACCZY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.004"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(11..850)
                     /gene="prmC"
                     /locus_tag="IVZ67_000147"
     CDS             complement(11..850)
                     /gene="prmC"
                     /locus_tag="IVZ67_000147"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8164253.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(843..1916)
                     /gene="prfA"
                     /locus_tag="IVZ67_000148"
     CDS             complement(843..1916)
                     /gene="prfA"
                     /locus_tag="IVZ67_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8164254.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(1919..2503)
                     /locus_tag="IVZ67_000149"
     CDS             complement(1919..2503)
                     /locus_tag="IVZ67_000149"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8164255.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(2609..3078)
                     /locus_tag="IVZ67_000150"
                     /pseudo
     CDS             complement(2609..3078)
                     /locus_tag="IVZ67_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3184..3786
                     /locus_tag="IVZ67_000151"
     CDS             3184..3786
                     /locus_tag="IVZ67_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8164256.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(3890..4459)
                     /gene="lepB"
                     /locus_tag="IVZ67_000152"
     CDS             complement(3890..4459)
                     /gene="lepB"
                     /locus_tag="IVZ67_000152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8164257.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(4689..5456)
                     /locus_tag="IVZ67_000153"
     CDS             complement(4689..5456)
                     /locus_tag="IVZ67_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164258.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(5453..5932)
                     /locus_tag="IVZ67_000154"
     CDS             complement(5453..5932)
                     /locus_tag="IVZ67_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8164259.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(6057..6794)
                     /locus_tag="IVZ67_000155"
     CDS             complement(6057..6794)
                     /locus_tag="IVZ67_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8164260.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            6955..8355
                     /locus_tag="IVZ67_000156"
     CDS             6955..8355
                     /locus_tag="IVZ67_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8164261.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            8578..9930
                     /locus_tag="IVZ67_000157"
     CDS             8578..9930
                     /locus_tag="IVZ67_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8164262.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            9923..10621
                     /locus_tag="IVZ67_000158"
     CDS             9923..10621
                     /locus_tag="IVZ67_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8164263.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(10683..11834)
                     /locus_tag="IVZ67_000159"
     CDS             complement(10683..11834)
                     /locus_tag="IVZ67_000159"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8164264.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(11847..12284)
                     /locus_tag="IVZ67_000160"
     CDS             complement(11847..12284)
                     /locus_tag="IVZ67_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8164265.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(12299..14065)
                     /locus_tag="IVZ67_000161"
     CDS             complement(12299..14065)
                     /locus_tag="IVZ67_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8164266.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            14329..15006
                     /locus_tag="IVZ67_000162"
     CDS             14329..15006
                     /locus_tag="IVZ67_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164267.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            15009..16136
                     /locus_tag="IVZ67_000163"
     CDS             15009..16136
                     /locus_tag="IVZ67_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164268.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(16336..16776)
                     /locus_tag="IVZ67_000164"
     CDS             complement(16336..16776)
                     /locus_tag="IVZ67_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8164269.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(16787..18820)
                     /locus_tag="IVZ67_000165"
     CDS             complement(16787..18820)
                     /locus_tag="IVZ67_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8164270.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(18822..20525)
                     /locus_tag="IVZ67_000166"
     CDS             complement(18822..20525)
                     /locus_tag="IVZ67_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164271.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(20619..21569)
                     /gene="manA"
                     /locus_tag="IVZ67_000167"
     CDS             complement(20619..21569)
                     /gene="manA"
                     /locus_tag="IVZ67_000167"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8164272.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(21608..22633)
                     /locus_tag="IVZ67_000168"
     CDS             complement(21608..22633)
                     /locus_tag="IVZ67_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8164273.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(22842..23711)
                     /locus_tag="IVZ67_000169"
     CDS             complement(22842..23711)
                     /locus_tag="IVZ67_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8164274.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(24415..26307)
                     /locus_tag="IVZ67_000170"
     CDS             complement(24415..26307)
                     /locus_tag="IVZ67_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164275.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(26304..28031)
                     /locus_tag="IVZ67_000171"
     CDS             complement(26304..28031)
                     /locus_tag="IVZ67_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164276.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(28047..28697)
                     /locus_tag="IVZ67_000172"
     CDS             complement(28047..28697)
                     /locus_tag="IVZ67_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8164277.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            28901..29197
                     /locus_tag="IVZ67_000173"
     CDS             28901..29197
                     /locus_tag="IVZ67_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8164278.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            29389..30009
                     /locus_tag="IVZ67_000174"
     CDS             29389..30009
                     /locus_tag="IVZ67_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8164279.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(30101..31267)
                     /locus_tag="IVZ67_000175"
     CDS             complement(30101..31267)
                     /locus_tag="IVZ67_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8164280.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(31279..31632)
                     /locus_tag="IVZ67_000176"
     CDS             complement(31279..31632)
                     /locus_tag="IVZ67_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8164281.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            31760..32782
                     /locus_tag="IVZ67_000177"
     CDS             31760..32782
                     /locus_tag="IVZ67_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8164282.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(33221..33655)
                     /locus_tag="IVZ67_000178"
     CDS             complement(33221..33655)
                     /locus_tag="IVZ67_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164283.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(33765..35624)
                     /locus_tag="IVZ67_000179"
     CDS             complement(33765..35624)
                     /locus_tag="IVZ67_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8164284.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(35742..36902)
                     /locus_tag="IVZ67_000180"
     CDS             complement(35742..36902)
                     /locus_tag="IVZ67_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8164285.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(37209..38633)
                     /gene="araA"
                     /locus_tag="IVZ67_000181"
     CDS             complement(37209..38633)
                     /gene="araA"
                     /locus_tag="IVZ67_000181"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8164286.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(38649..39347)
                     /locus_tag="IVZ67_000182"
     CDS             complement(38649..39347)
                     /locus_tag="IVZ67_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8164287.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(39304..40965)
                     /locus_tag="IVZ67_000183"
     CDS             complement(39304..40965)
                     /locus_tag="IVZ67_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8164288.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            41183..42583
                     /locus_tag="IVZ67_000184"
     CDS             41183..42583
                     /locus_tag="IVZ67_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8164289.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(42835..43914)
                     /locus_tag="IVZ67_000185"
     CDS             complement(42835..43914)
                     /locus_tag="IVZ67_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8164290.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(44000..44959)
                     /locus_tag="IVZ67_000186"
     CDS             complement(44000..44959)
                     /locus_tag="IVZ67_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8164291.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(44971..46494)
                     /locus_tag="IVZ67_000187"
     CDS             complement(44971..46494)
                     /locus_tag="IVZ67_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8164292.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(46504..47067)
                     /locus_tag="IVZ67_000188"
     CDS             complement(46504..47067)
                     /locus_tag="IVZ67_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8164293.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(47073..47936)
                     /locus_tag="IVZ67_000189"
     CDS             complement(47073..47936)
                     /locus_tag="IVZ67_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164294.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(47950..48846)
                     /locus_tag="IVZ67_000190"
     CDS             complement(47950..48846)
                     /locus_tag="IVZ67_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8164295.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(49021..50292)
                     /locus_tag="IVZ67_000191"
     CDS             complement(49021..50292)
                     /locus_tag="IVZ67_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8164296.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(50282..51799)
                     /locus_tag="IVZ67_000192"
     CDS             complement(50282..51799)
                     /locus_tag="IVZ67_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8164297.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        14899 a        10728 c         9076 g        17235 t
ORIGIN      
        1 atccacaact tcaaccataa acttggatca ttcgttcatt gccaaaaaga tcctttttta
       61 tcgccacttt tttatctgga aatgcttgct gaaatatttt ttttactgct tcaccttggc
      121 gaaatccgat ttctaaaaaa atctttccat ttgattttag tacgcttggt gcttcaactg
      181 ctattttttc ataaatagct aatccatcgt tctcagcaaa aagggccatt ttaggttcga
      241 atgaccggac actttcatcc atcagttccc attcattccg actgatgtac ggcggattag
      301 aaataatcac atcaaattgc tgatccatta ctggtcctaa tgtatctcca tgataaaaac
      361 tgattttcgt tccttctttc tgagcatttt ctttcgctac ttctaatgct tcttctgaaa
      421 gatccacagc agatatatgc caattttttc ttgctgcttt caaactaata gcaattgctc
      481 cagtccctgt cccaatatct atcacttcta acggtacacc aggcgtctcg tctaaacacc
      541 attcgactaa ctcctctgtt tctggtcgag ggatcagtgt tgcttcagtt acctttaacc
      601 gatgatcata aaaatctgca tatcctagca aatactgagg tggatgatct gcaagcagaa
      661 gtcgcagatc ggtttcgatc aaccgctgtt cttcttctga aatctcttcg ttcatatgaa
      721 ggagccaatc aagttttttc cattgttttc gttccaaaaa taaaaattgg atactatatg
      781 cttctttccc atgatcctct aaaaaagaag aagcccgtga caggacttct cgataggtca
      841 ttttacccat tttgcatagc ttccagtttt gatgtttgat catacatgat caatgcatca
      901 acgatctcat ctaatttccc tgctaaaatc tgatctaatt tttggatagt taatccgata
      961 cgatgatcag tcacacgatt ttgcgggaaa ttatacgtcc ggatccgttc tgaacgatcc
     1021 ccagttccga ctgctgattt tcgattggca tcatactcgc tttgtgcttc ttgctggatc
     1081 ttatcgtaca cacgagcacg caagattttc attgcttttt cacggttctt gatttgagaa
     1141 cgttcatctt gcatagcaac aacgatccct gttggtaagt gagtcaaacg tacagcagat
     1201 gctgttttat tgacgtgctg tccgccagca cctgaagcat gatagatatc tgtacggata
     1261 tctttgtctt caatatcgat ctctacttct tcagcttctg gcatcacgac aactgttgcg
     1321 gtggaagtat ggatacgccc ttgcgattca gttgaaggaa cacgctggac gcggtgcgca
     1381 ccactttcat attttaattt cgaatatacg ttgtctccgg tgatcatcat gatcacttct
     1441 ttatatccac cgattccggt aacgttagct tccatcactt ctgttttcca gccttgtgct
     1501 tcagcatact tttggtacat attgaaaaga tcgccagcga ataatgctgc ttcgtctccg
     1561 cctgctgctc cacggatttc catgatgata tttttatcgt catttggatc ttttggtaaa
     1621 agcaagatct tgattttttc ttctaagact tctttttctt tttttaattc agataattct
     1681 tcttttgcta gttcagccat ttcatcatcc agtttttcac caagcagttc ttctgtatcg
     1741 gtgattcctt gtactacttc tttataacga cgataaactt cgactgtttc gcgcgtgttt
     1801 gcttcttctt ttgaaagctg cataaaacgc tttgtatcag aaatcacttc tgggtcactt
     1861 aaaagttctc caagctcttc ataacggtct tcaattgctt gtaattgatc gtacatgctt
     1921 attctccttc tgtaatcatt gatgggtgaa agtaatgttt tcgacaaacg gggtaataag
     1981 cttcatttcc gccaatttgc acttgatcgc cttcataaac agggtttcca tcaatataat
     2041 gtaaattcat gattgctttt ttgtggcaga accaacagat tgttttcatt tcttcgattt
     2101 tgtctgcata aagcaataaa tattttgatc cttcaaataa ctcgtttcga aaatcatttt
     2161 tcaaaccaaa tgccataacc ggaatatcca attcgtccac aattctcgta aaagcaatga
     2221 catgtttctt ttttaagaat tgcgcttcat cgatcagcac acaatagggt ttgctttcca
     2281 ttttctgaat tacgtcaaat acgtctgttt cttcaaatat cggcatcgca ttacgtttca
     2341 gtccgattcg gcttgaaaca acgcctaaac catctcttgt atcaataccg cttgtcatta
     2401 agacaactgg cttattttgt tcttcataat tatgtgcgac ttttaggatc tcgatcgtct
     2461 tgccgctatt cattgcgcca tacttaaaaa atagttgtgc catgctcttt ttcaccttcc
     2521 agtttgttcc tatggtatta tacttgattt tcagcatttt ttacagtgag aattttcatc
     2581 aagcacagat tagtattaaa tctattgctc acttttgttc caattttttg acaagaacta
     2641 aacatgggtt ccattcatcc cataaatttg gaaaaacttc caaccttgaa aatcctaatg
     2701 attcgtagaa acaaatagtt tgatcatata tagagtagtg cccctcgtct acagtcttca
     2761 cttgcaaata tttgtaatgt tttgctgcgt aaccttctgc ttctttaaaa agttttgttc
     2821 ctaatccttg gtgatgatag tcttttttta ttcccataca atagaataga tttcggcagt
     2881 atcttcactt gtctgtgaaa gagtgataaa gcctaaatat tcttcttttc ttttttcaca
     2941 ccataaaggt agtttacttg attcctcgat atatttttcc gttgattcag gaagcccaaa
     3001 ccactcaggc aagtctagta atatttctcg gacacacttt cctttttcct ctctatcttt
     3061 tacaaacgat atcggcatat tctttctaga ttctcccttt ttgaattgag tatagcatgc
     3121 tttctttctt ttcacgtgct ataataagtt aaaatttcaa agttttacgt aaggagctat
     3181 ttgatgacaa aaaatatttt tgattccatc gctaaccatt atgattcccc tgaccgtcaa
     3241 gcattagcca agattattcg cgaagaacta actacttatc taccgcgcga cagccaccaa
     3301 aaagtcctgc tggattacgg aggcggaact gggcttgtta gtttaccgct tgcggaaaga
     3361 ttcaaagaac tgatcattgc cgatgcttca gaaactatgc tgaaaatggc agaagaaaag
     3421 atccaagcag cagatctgaa aaatgtccga acgatccacg ctgatgcttc tgtcgaattt
     3481 cctgctgtcc aagctaatct catccttcta tcacttgtac ttttacacat tcctgatacg
     3541 gagaatattc ttacaaaact ttatgaaatt ttagctcctg gcggtcaatt gatcatcgta
     3601 gactttgaca agaatgaaca aattagtcat ccgaaagtcc ataatggctt tagccaagaa
     3661 gaattgaata atcgattgga aaaaacgggc tttgtctctg ctactagtca taccttccat
     3721 cgtggggaaa agctatttat gaacaaaaac gcttcgcttt tcttatccat cagccaaaaa
     3781 gcttaaaatg agattgcaaa aaaagtgagc aactccaaga aataagaagg aattttcgga
     3841 aaaagaagct gtgacaaaaa acatgtcaca gcttcttttt attttttcat cacatgattt
     3901 taatttctga aagaggccaa taacggaaaa caacttttcc ttggatcata tctttgtcga
     3961 ctaacccaaa cattcggctg tcttttgaaa tcaagcggtt atcgcctaga acaagatact
     4021 ttccttttgg cacagtatat tcaaagtcac tcgtaaaggt ggatgaactt tctgcttgtg
     4081 cttggaatcc tgagctataa gcatattctc cttgtaactt atcttctgaa aattcttttt
     4141 tgaactcatc cagataaggt tcatcatact ttttgccatt aatggtcaac tggtcatttt
     4201 ccatcttgac tgtatctcca ggcataccga tgatccgttt aacaatcatc cgttcttcat
     4261 cccccggttc ctttgtagta ataatatcca ttcgttcata gtttgagatc ttagatgaaa
     4321 tcaatcgttg tccatcactt aatgtagggt ccatcgaatg cccgtttaca gtaactggtg
     4381 taaacacgta cactctagct aataaaatag ccgctaatac aaggatcaaa atcccccaat
     4441 ttttcaaaaa ttctttcaca gcttacctcc tacttgtatt ttcctcgtta tttattacgt
     4501 tttttcacaa tacttcgttg tactttcttt attctacaat gtttataaat gaaaggaaag
     4561 aataagtgat gtgttcgtct tatctttctc gttatataaa aaaggaacgt gacaaaagac
     4621 gtgtcacatc ccccttattc cgaataaacg tttttatcgc aaccactgat acacggcgtt
     4681 tcatcacatt atgaatgtct tcttctttgg ctacgactat aaaccactgg ttcttcctgc
     4741 gactgtgcct tttttgcaat caatttcaga cgataaggct catgatagcg taacccgtct
     4801 ttgacaaaat caccgccgac gatgaacaat ccgacaggtt ccaatgtgtc ttctgttgag
     4861 tcaagtcgaa tggtccctac tttttccatg tccgagtagc ttcgtccaat atttagaaca
     4921 tactctttct ctcctgtttt cttgacatag taataaggat acaatgtatt tctatcgacc
     4981 agaaatccat ccatgcgacc gagttcattt gttttgatct caccatttcc aatcaaatac
     5041 cgcatattct cggctaattt caattgataa tcttcgtcat aagaaaccat gacctcttgt
     5101 gtattcactt tatcgatcga atttccatta tctatcgatt cttctgtctg gatgttgaac
     5161 gcttcatcag gtaaatactt cacaaaaagc tgagtgacat cggctttcgt gtttttatct
     5221 aaaaagtaat aaaatacatt taaaacagta aaataaacaa gcactagaaa tgcaagtcca
     5281 tataagaaaa attgaagata gttttggttt tgccaaagtc cataaacaat tctcaataaa
     5341 taaagcgtgg gaatcacact taaaatcgta tatccacggt taagatattt aggactgatg
     5401 tctaaatagc caaggaattt atgcaccccg ttgataaggt ctagtaaaaa tgtcattgag
     5461 aatcacctgc ttgctggtta tacgtttggg tatttcctga atcttggttt tgctgtgctg
     5521 tacgtgctgc atcatcattt tgcgtgttat catctacagt cgtactgctt ggtatcgttt
     5581 cggagctagc acttgtatcc ggacttgtac ttggattggt tgaatccgat ggagtgccat
     5641 ctggtaccgt attacttgtt tcgcttgagc tgtcagttgt atatgtctca tcatacgacg
     5701 aactagttgt tgtatcgtcc catgtacttg tcgtagtggt ttcattcaaa ctttccgttg
     5761 ttgttggttc agatgtcgag actgaagtag ttggtgttgt ttcttctaca gtactagaac
     5821 ttgaacttgg tgcacggtag ctggaactat tcacaatacc tgtagtagtc gcgacaatcg
     5881 tcgagattgt taatacagcg attggcttca aaataggtgc tacttttcgc atcttatctc
     5941 ttccttcatt gaaatttatt cttgtttcct tactttacta cggacaaatt atacagtcta
     6001 ccttatttct accataaatt ctttattttt ttcttacgtt tttccgaaag atttctttaa
     6061 ttttcctcaa aaactacggt tgatgttttt aagtaagctt ttgcttcccg attgatttct
     6121 ttcagatcgc atccttgctg cttggcagcg aacacgcaac cacaataaca ttggcgataa
     6181 atatcatact ctttgcacat ttgcaaagag cgttcatagc ctttgttttt cttgaaatca
     6241 cttggcaagt attgtgtccc gtaaatcttt tggatatcca ttccgatctg attgatcaaa
     6301 gctgcatttt tctttggcga gatggtcagg gcacttccaa agtaatcgta tcctagttct
     6361 tgcgcttttt gcgcaacaag atctaatcgc atattgaagc atgcactgca gcgagcacca
     6421 ccttcttttt cgttttcaag atggtttgtt aaaaccattt tggtgaattc atttggacgg
     6481 tattcatcaa cgattagcgc aacatgatgg tcagttttct cattaaattg gtctacaaat
     6541 cgtttttgtt catgtaatct tcgcaaatac tcacttttag gatgaatatt cgaatttgaa
     6601 aaaaataacg tgatctctgc atattgagac atgaattcca atgtgtgcgt actgcaagga
     6661 gcacagcatg tatgtattaa tatcttaggc tttacttctt ctttctccca ttgcatgatc
     6721 atttttttca gaactgtatc ataatttatt ttttgatttt tcattttttc tacgatttgt
     6781 tgagcattaa tcaatttgtt attcctcctc cttctttttt ctaggatttt ccttattata
     6841 agccatttct attttaatcg aaagctgtat tgttatctgt ttgataattt ctttgagtat
     6901 tcctaattcc ccttgtatac tggtagaaac gactattgtc aggaggatag tactgtgcat
     6961 gttgatttat tctttcgaag gctgcagcgg cgaggacaga agtttgctgc caatcatttc
     7021 tcatcgatcc aaatcatcgt tttttattat atattgatga cggtcttatc actcgttcta
     7081 ttttatatgc cgatttttcg cgaaccagat agccatgttt cttttgtgga catgctgttc
     7141 atggcaatca gtacagtgag tgtgactgga ctatcgactt ttgatatcaa ttctgtgttc
     7201 aatgataacg gtattatact gctggaaata ttatttcaag ttggtggttt ggggatcatg
     7261 atgatttcta ctgcttttgt cattttttct aaacgacgga tcacactgaa acagcgacag
     7321 ctgatcatga cggatatgaa tcaaccgcgt ttgtctggta tcgtccgact gattcggatc
     7381 acttttgcta ttttaatctg gtttcaatta ctttttggga cattcttttc tatttatttt
     7441 tattacagag gctattttga tcgttggcgt gatgccgttt tttacggttt ttatcaggcg
     7501 atatcggctg tcacgaactc tgggtttgac gtcactgggg attccatcaa gccctttgca
     7561 catgactatt ttttcttgat cctgatcatg tttttgattt ttgtcggagg aattggtttt
     7621 cctgtattga tggagtgccg ggaatggcta ttatacaaac gttccaatgc gaagcttccg
     7681 tttcgttttt ccttgtttac caaattagct gtgttagctt ttgttatctt gtttgtttcg
     7741 ggaactgtct tgatttatct attggaaaag gaccatttat tccaagattc caatatgagt
     7801 gtcaaatgga tcaattcgat gttttattcg attacgactc gtaatgctgg tctgcagatt
     7861 cacgatttgg gtgattttca aatcacaaca ctgatcattt tttctttact gatgtttatc
     7921 ggatgcagtc ctagttctgt aggcggcggg attcgaacaa ctactgtagc aatcatcggt
     7981 ttgtatctat attccttcct caaaagtgaa gataacatca atatttttgg acggagaatc
     8041 gatcaagatg atgtgcgaaa atcagtcgtt gtctttatgc tttctcttgg tatgtgtttc
     8101 ttttgtatcg tttttttatc tgctacagaa gaacaaacgc tgatctcgat catcatcgaa
     8161 gtaacttctg cgtttggaac aactggttta tctttaggga ttaccggaga cttgtctgtt
     8221 gtgggcaaaa tcacgattgc tacactgatg tttattgggc ggatcggaat gctatataca
     8281 ttgatgttat tcgtgccaaa agaaacacga gatctaggct atgaatatcc aagcgagaaa
     8341 attattatcg gttaaaatgg gaatataggt aagagggaat aataaaatca cagcagcttg
     8401 aaacaatcat tttgtttcaa gctgtttttt tgttaaatca tgcgattttg tccgatttca
     8461 tgcactttca gccgtaacct tttcttaaga ataagaaaag caatttaacc tttttttcaa
     8521 catatggtat gattagttga atttattcgt cagcaaatcg tttttggagg ttacataatg
     8581 ggaatacgca gtcattttgc gattgcagtt ggaaaaacgt cgcaatgggc attaaaaact
     8641 ttttttaaag gcggatcaag tttaccaggt aaactagcgc tgaaagtcga tccacatata
     8701 ctagatagtt tggcaaaaga ctaccaagtc gtcgttgtca ccggaacaaa cggtaaaaca
     8761 ttgactaccg ctttaacagt caatattttg cgccagcaat tcgatgaagt tctaaccaac
     8821 cctactggtg cgaatatggt acagggaatt gtatcgactt tcttgcaggc aaaaccaaaa
     8881 aaaggacaga aaaagtttgc tgtcctagaa atagatgaag caagtttgag caaagtcaca
     8941 gaatatatga aaccagaact gttcttattt acaaatattt tccgtgatca aatggatcgg
     9001 tatggagaaa tctatacgac ctataaaatg attgtagacg gtgccgcaaa gtcaccaaat
     9061 gcaacgatta tcagtaacgg tgattcacca atcttcaatt ctatcgaaac agtcaatcct
     9121 agaaaatatt atggctttga ccatgaagaa gatcgtgaac aaatggctca ttacaataca
     9181 gacggtgtct tatgtccaaa ttgtcatcac atcttgcatt ataaaatgat cacttatgca
     9241 aacttaggga aatattactg tccaaattgt gacttcaaac gtcctgaatt ggattatcga
     9301 ttaactgata tggtacggat ggacaatgtg tcagcagatt tcgtgatcga tggtcatgaa
     9361 tacggaatcg aagttggcgg tatgtataat gtctacaatg ctttagcagc tactgcagtg
     9421 gcagagtact tcaatgtcga tcctgataaa atcaaacaag gtttaggata tgacgagaaa
     9481 gtattcggcc gacaagaagt catcaatatc aatgggaaaa aatgtacatt agtacttgtc
     9541 aaaaatcctg taggtatcaa tcaagtaatc gatatgattg gtctagctcc ttatccattt
     9601 tctttagtgg ctttattaaa tgccaattac gcagacggaa tcgatgtcag ctggatttgg
     9661 gatggtaatt ttgaaaactt tgccgatatg gatatcccag ctgttatttc tggaggagac
     9721 cgtcatacag atatgacttt acgtctaaaa gttgccggga ttccagaaaa taaattgaca
     9781 gaaacaaaag aattaccaga agtaatcgaa aaaatcaaag aattgccaac tgagcatgtc
     9841 tatattttag ctacttacac tgctgtattg cagcttcgta aagaactagc tgctcaagga
     9901 tttatcaaag gaggaatgaa ccgtggctaa ttatgaactc aatatcgccc acctttatgg
     9961 aaatttgatg aacacatatg gtgataacgg aaaccttttg atgttgaaat atatagcaga
    10021 aaaaatgggg gtttcttgcc atacggagat cgttagtata catgaacctt ttgatgctga
    10081 taaatatgac ttggttttct ttggcggtgg tcaagacttt gagcaattaa tcatttcaaa
    10141 agacatccag aaaaagaaag attctttgat caactatatc gaaaatgatg gcgtcatgtt
    10201 agccatttgt ggtggctacc aattgctagg tcactactac atgggagcta atggagaaaa
    10261 gatccaagga atcagtgcac tggatcacta cacgttaagt caggaaaata accggtttat
    10321 cggtgatatc gttatccata acgaagaatt caatgaaact tattatggat ttgaaaacca
    10381 caatggtcgg actttcttag gtgaaggtga acgacctttg gggaaagtgg tacaaggtca
    10441 aggaaataat ggtgaagacc aatctgaagg tgtcatttac cgaaatgtct tcggttctta
    10501 tttccatgga cctatccttg caagaaatga gaacctggca gctcgtttga tccgcctagc
    10561 tttagaacaa cgttacggta aagaattgga tctgccctat cttgaaggac aaacagccta
    10621 gttattcgaa aaaggggaat gtgacaaaaa tttcgtcaca ttcccctttt tcgtatccta
    10681 ttttaagcga cttgcttggc ataacggttc acgttttgct tgatattccc gattgttttc
    10741 ggatcattga ttcctgtgac ttcttgaatc aattgttcca agttttcatg gatcttcatt
    10801 tcatgcaatt gacggctttg ttcatcttca ggatcatagt aattaaaaat aatccccatt
    10861 gttgccgtca aatgtgggca agtcaagtta tgttcttgta gttctctcac tggacgtgta
    10921 aatctttctt gataacctaa cttacgtaat ggtgtccgag cgacacgtgt gatcgcatca
    10981 gagatatttt tgttttggaa gcgatgaatg attttttcaa tatacgcatg atgctgctct
    11041 gcatcaaacc cccatttggc aatcagcaaa cttcctgttt cctgcaaaac tgattttaat
    11101 tgtgccacaa ctaacgcatc ttgcattgct tcatcaatgg tctggtaacc taacaaggca
    11161 cctgtataag cgacagtcgc atgaccagta ttgacactaa atagtttgcg ttcgatatat
    11221 ggttctagat catctacata taaaacccct tttaattgga tttttttatt ttttctttgg
    11281 ctatcgtcaa tgacccattc tttgaaaggt tccacttgga cgaataaagg atcttcatgg
    11341 tgttgtaatg ggacgattcg atcgactgct gcatttggaa agccgatata tttgtcagta
    11401 aatgatgttt ccggaagata tttatacact tctttttcta gaaatgctga accgccaatc
    11461 atattttcac aggcaatgat atctaaagat gttgtgcttc cttccttttc tctttcttgg
    11521 ataccttttg caatcaactc agcaataaaa ggtaagatat tcgggccaat ggcggtagta
    11581 actaaatctg cttcagcgat ttcttggatt actttttctg gatttgtttg attgttgatt
    11641 cctttgacat tttttacagt gattttttct tttgattcat ctgctaattc gatttcgtat
    11701 tcttttcttt catttaaagc atcaatgatc gtgtgattca catctacgaa agtaatctca
    11761 aatccattag ctgccaaaac ctctccaata aatcctcgac cgatatttcc tgcaccaaaa
    11821 tgtactgctc tcatttttct ctctccttaa gcaattgcta gattttccgt tacttcttct
    11881 ttgctgagcg cgtctgctag ctggacaaca ttgtccatat cactgcaata aagtgcgatt
    11941 tgctgtacaa gttgcaaatg ctcatctccg actcctgcaa taccgaacaa aacagtcgca
    12001 atcttttctt cttcttcggt accaaaatct acaccatctg gtacttgaac gacacagatg
    12061 ccggattttt tcacatattt ttttgcttca tctgttccat gaggaatcgc gataaaattc
    12121 cccatataaa cagataacat tttgtctcta tctaccatgg catcgatata ttcctcatca
    12181 acgcaccctg cttctacaag catctgaccg caatagcgga tcgcttcttc tttggtttta
    12241 aaagatcgat tgagctcgat catgtcgaat ggcaaacttt gcatgctgtt ttcctccctt
    12301 aagctgtctg tttcaaatat tgtgcaatcg attcgtattt ttgcggattg accatgctgt
    12361 tgactgataa atgaatggat tgaggtgcag ccatctgtac tttttccgct aaactttctt
    12421 tggctacgat cagtgaatca ggttcatctt ttaaatcttg gatattgact tttcttaacg
    12481 ggatagttaa atggttcttg tctaatatat ctttgagcac ggtcatcccc attgttgctg
    12541 atccggaacg atcatcgtgg acaagtagaa tctctttgat ttgtttgatg tttgctgaaa
    12601 gaaactcttc tgctggctca ttctcaattt cagaaactga tgttttctgc ttgctgctta
    12661 attcttgaat gatttcatca tatcgaggtg aattcaaaaa gttttctact gagacaaaac
    12721 gcgcgctcgg tgctttttgt tttgctcttt cttgtagttc gacctgagta acgatcaatg
    12781 tattcgagtc atctgtcagt tggctgattg cttgattggt caccgtttga ggcaagtctg
    12841 cttcctgtac tttttttctc aagatcgaag cgcccatcgc acttgatccc attcctgcat
    12901 cacaagcaaa aatgattttt tgtacgccag agaatgtagc tccttgagga ataccttggg
    12961 cacctttgga agcttgctta gctgcctttg tctcttgaac agatttttcg aagtcttcat
    13021 ttgtatcttt cgcatccgct ttcaaaatta ctgcagaaat accaaacgaa acagctgcgg
    13081 caacaatcac gcccaaaata acaggaaggt aagaattgaa cggtgtcatc gctagaatcg
    13141 caatgatcga ccctggagaa gctggcgctc ttagtccagc atccatcaat tggaaaatga
    13201 aacttcctga cattcctccg cctataactg ataagaataa taacggtttc atcataacgt
    13261 aaggaaaata gatttcatgg atcccgccta aaaagtgaat gatcatggct cctggtgctg
    13321 aagattttgc tgctcctttg ccaaacaaag taaaagcaag taatacgcca agtcctggac
    13381 caggatttgc ttcaagcaag aatagaatag attttccagc ctctgctact tgttcagttc
    13441 ccaatggtgt caaaatccca tgattgattg cattattcaa aaataagatt tttgctggtt
    13501 caataaaaat acttgtcaat ggaatcaaat gggcagataa aatgatctct acaccttttg
    13561 ccattgcttg agtcaatgca tcgacaactg gaccaatcgc agaaaaacct aataacgcta
    13621 atccaaaacc gataagacct gctgaaaaat tattgactag catttcaaaa ccggatttga
    13681 tccgtttttg gaatagctga tcaaatttct tgatggcata tccaccaaga ggtcccatga
    13741 tcatcgcgcc tagcatcatt ggaatatctg ttccgacaat cacacccatc gttgcaattg
    13801 ccccaacaac tgatccgcgg tcaccagcaa ttacttttcc gccagtataa ccgatcaata
    13861 aaggaatcag ataagttagc attgggctta ccatggtagc gaaagcctca tttggtaaat
    13921 aaccatctgg gataaataga gccgttaata caccccaggc aatcagtgct ccaatatttg
    13981 gcatgaccat gcttgataaa gtactgccta atttttgtac cctcgctttt agcgaaatgt
    14041 tgtttttggg aatcgtccgt tccattttgt ctctccctct ctccttttga tactctaatt
    14101 gtatccgctt actaaaaaga gaaatagtcg tactttggca cgaaactttg tgccaaaggt
    14161 ttgagtatgt cagcaaaata aaaagatttc ggtatcagaa aacttcaatt ttaaataaaa
    14221 ctgttatttt aattttttta taatatgcaa acaataactt ttgttagcat ttaatttaga
    14281 tgttttccat tctattatgt tattaaattt tatattggag gcctatctat gacattactg
    14341 atcaaaaaca tgaacatgaa acgtggtaaa aaacgtgtga ttcgacaatt gaatctttta
    14401 gtagaatcag gagaagtagc tgctttgatt gccccgaacg gatttggcaa aacaactttg
    14461 ctgaatggca ttgcccaact gctgccaaca acttatgatc aattgtccct tcacaacatc
    14521 tctcctactg cttatacaga ttatcgccgg tctttctttt ttctagaatc aacgaatcag
    14581 ttatccgact atctatcggc tgttgattat ttgactttga tcaaatacta ttggcagagc
    14641 aaagtagaat gccaaactgt tttccaacga ttgaagatga ctgagtacct tactttgccg
    14701 attggaaaaa tgtctctagg aatgaaacaa catgtcttgc ttgctgctta cttgatcagt
    14761 gattcatctg tcatgttatt cgatgaaccg ttgaacggat tagacccagg aagtattgat
    14821 atcttgaata gcctcatcag acaatggcag aaagagggga agacgattct tctttcttcc
    14881 cacgctatcg cgaatatcca atcgatttgt tctaaagcat gttttctaaa aaatggagaa
    14941 atcgtcacag aatcctcaaa tatggaagag attttagcta tttatcgtaa attatatgag
    15001 gtgtaactat gtctttactt acatttgaac gcaatctctt actaaaaaac aaagtaaact
    15061 ttttatttcc tctattgttg gtcgtgctct ttgcattccc actttttttt gatcataaac
    15121 ttgcttatac agaattcgat gaactgaatc ataactatga agagatgcaa cgtttgattg
    15181 aaacgttgaa agaagatgag aatgagaaag aatttgtgga aagtttagag aaaagcaaca
    15241 aactgatcga agcaatctta catgcaaaaa acacagggaa tgtccagcaa atggtcgaag
    15301 caacttatca ttatgaaaaa gatatactgg atcgactgat ttctggtcaa agacaaggaa
    15361 ttccaatcat tgaacaacaa aaacgtgtcg agttgctccg ttatatgaaa gaacatcaga
    15421 tccaaagata ttctattttt gacttgccag cgcatctctc tttagcaaat tactacgaaa
    15481 atattttttc aggaatgatt tctagttttt tgattttatg tataaccgcc ttgtttcttt
    15541 cttccatcat ctcttatgaa aaaagaaagc aggtaatctc tctagtcaat ctattactgg
    15601 atagtatggt aaagaaacat agcatccgtt ttaccattta ttatggtgca gctatgctaa
    15661 gtctcgtgat gccatttttg atcgtctcta tactagtaat aatcaaaaat ggtttgggag
    15721 acttccgtta tcctgtagga acgatcattg gtcaggagat acgaatcctg ccgatgtatg
    15781 agtatctttt ccagagcttt ctgttcctgt tgttgtgggt gctcttttta agtacaatca
    15841 gttttcttct atctgcttta tttgaacatt ctctagtcaa tcttttaggt acacttcttt
    15901 gtcttttctt agcagagtat cgcttgtttt cttcgattgg atggatagaa tcaatcagtc
    15961 attacttgcc aacttcttat gttgattttc aaaatgtgat cataggagga gatattttct
    16021 cgccactagc ttcagaacag gttactttca tgaatggtat cttgacactt ggtatatgga
    16081 gtatcgtttt attattcata ggtatgggca ctatctatat aaaaaagagc tattaaaaca
    16141 caaaaataat aggggcggtg acaaaaacct tgtcaccgtc ccctatttca aataaacggt
    16201 tttctagaat aaatagtttt caaaagcttg catccgcagt aataagaacc tcccaacaaa
    16261 aatagctcct cttattttta tcttggttga agttattact tgatgcaagg cagctttttt
    16321 tactgtgaac attggttata tatgtccatg ttcaaaaatc atttggactt cttcaatggt
    16381 ttccgcatca ctgagcgcca ttacttggtc gatgtctgaa cagaaaaagg ctaaatcttg
    16441 gagcaccgct aactgatgtt ctttttttaa agccactaca aataagagtg tcgcgatttt
    16501 tggttcctct tttgttccaa agtccacacc atcaggcact tggactaaat agattccttc
    16561 ttctactact tcttcatctt tgtcagtccc atgtggcaag gctacgaaat tcccgatata
    16621 gacactcgct ttttcttgtc tttcgatcat tgattggata tatgccggtt tgattccccc
    16681 ttgttgggtc atccactggc cgatctcggt caacgcttct ttccaatttt gataggattg
    16741 atcgagcaat attttttctg ctggtagctg tatcatcttt tcaccttcat ttctttattt
    16801 cttcaatcag gattttcgct aacaactgat aaacgatttc tttatttcct gagcggaaga
    16861 tctcggtgtt caagtcattc atgatgattg ctccactgat ctttcctaaa agaatcccgt
    16921 ctttttcttg gattggcgaa ggcgccagca taactaacat acgggacaaa tcgactggct
    16981 gtctatccat cccttcaatg gtcaacggat gtttcaaatt gaaaatacaa aagatcggtt
    17041 tagaaatcaa tgagctggat gtatggaata accccatatt tgtatgagga ataccgatag
    17101 gtgcttgttc atatcgctta agcagccggc gatgaaccac ctcttcatcc gttactagtc
    17161 cttcaggaaa ataaggaaat aagctgcgaa tcgtttcgga aatcgtctca ggattatcca
    17221 gttcttggat aaaaaaacga ccgagtaatt gattgatttg atccatgaaa tgcattgttt
    17281 cctcataagt atcttctatc atccactcgt tattgctgtt atacgtaatt ttttgccttt
    17341 ttttcttctc aatatgcgaa aaagcttctt ttagctgctt gacttcatct tctagcagta
    17401 gcggggagac taaaatatat tttccaccgt atcctggtaa aatagatgta gaaataacaa
    17461 catcatattc ttcttgtaaa tcgatttttt ttaaatcagc aatccgatag aaatgaattt
    17521 gttggatgaa gggaaaatac cttttcaatc gtagttccaa catgctagta gaagctaatc
    17581 cacttggcga aaatcctgca atactgatat tgctttcttt cggacttcgt tccaatgaat
    17641 tcgcaaaatg aaggaccata taagcgatct cttcttcgga taattttttt tcttgaaaaa
    17701 tccgctctac tgctttacgg attgcattcg caacttcctc gtattgaacc atgatttttt
    17761 ctaaaatcgg attggtcagt gtctcttctt gtaaaacggc tcttgaaaac acaccagata
    17821 aatgagtcaa cagcattttg tacaaagttt catcttcata aaaatggaca ttcgtctctt
    17881 cacttacttc ttgaatcagt tgttttacta aataagcaag ctgtgtatcg aacgtctctt
    17941 caaagaaatt ctgatcaaat gagttcgtga agtcatttaa taatcctgca aaaaagacga
    18001 tctcatttac tggataaagt tgttttgtct cagaagcgat ccgtgaaaac aatcgtttag
    18061 ctgtctgtag taattctttg tttgccagac tattatatgt ctcatttgtc agtaaatgat
    18121 ttttcgtcac acgctccatc gtcaatgtca gcatcaaaac caaatgttca agctttctat
    18181 cacttagatt ggtctttttt tggatcaatt cttccccgat tactttgcgt gcaagagata
    18241 gatgttgcgg atcaacgaat cgaaaaaaat aattcagtga tgcatcgaaa gaaatactat
    18301 gaaaaagttc atattcattg atttctaatt ctaatacatt cgctgtcaga agccggcggt
    18361 atttttccgg tccttcgatc tcgtatccct ggttccgaat gatttccaaa ggcagttggc
    18421 gaatactttc ttccaactgt ttgatatctg cataaaaagt ggtattactg atgcgatatg
    18481 attccatgaa atgagcaaga ctcaacggct ctgtcgtcag taacaactcg gctaaaatcg
    18541 cgtgttgtcg tttagcagtg gataagtcat tacttttccc atctgtattt tcttttaatt
    18601 gctctaatac ttcttctgat gcctcaatga caaatgagcc tctagctgct tttttcaaag
    18661 aagcatttac cgatttcaat gagacttcaa gattatctaa ttcgcggtaa atagtccgtt
    18721 tactgacctt caataaattc atcatttttt ggatagatac aggtgacggg ctactgagaa
    18781 attctgctaa taattgtttt tctctgggcg ataaatacat cttatatttc tcccttcacg
    18841 agtttttcgt attcattcgt agctacccaa tgttcaacta gaactagccg agggatcttt
    18901 tcttctagct gttcagttac agctagttct ctttgtgcga tataaatcgt ttcttcttgg
    18961 attttgattt tttctattga ttcttttgag ataacaaact gttttttcgc tttttttgct
    19021 aattgactta aaatcgtcat gcccatagtt tgtgagccgc gtctgttgtt tttatacaaa
    19081 aatacaatat tagcaggcga cgttttttgt tctgtgatat ctgctatctc ttttttttct
    19141 tcccgctttg gaaattggat cgcttctttt attcgtgaca gcaagatatc tgtttccaaa
    19201 aagtgatcca gtacatagat ttgactttga ggtacttttt ctcttgcaag acccgctagt
    19261 tcatgttgca cgataaccag ctgatttact tggttattca attcatagat agagctgtat
    19321 ccgacagaaa gatcgatttt ttctgctgct aatttctttt tcagcagggc ggctcccata
    19381 gcacttgatc ccatacctga gtcgcaagca aaaatgacgg aatgaatgat agatggttcc
    19441 aagatttctc ccctttcttt gaccgttttg ttctcttttt ctttatttgg taatttcaat
    19501 agtatcgctg cacaaatgaa ggtcacaaat gtactgattg ctatccctat acttacccca
    19561 gctagttgtg gataaggcgc attggcaaaa atcacgagta gtgacccagg cgatacaggt
    19621 gcagctaatc ccgtatctaa ccattgaaat atcaacgtgc cgcttgcccc accagccatt
    19681 acagctaaaa atagatacgg tcgcagcaag acaaatggaa aatagatttc gtgaatcccg
    19741 ccaatcgcat ggatcatcaa tgctccactc gcatttactt tttcctgttt ttctgcaaaa
    19801 caaaggatag aaagtaagat ccctaaacca ggtccaggat ttgcctccat caaaaataag
    19861 gaagagtacc cgactgtttg tgagaattcg attcctaaag gtgtcaatac accatgattg
    19921 attgcattat tgaaaaatag tacttttaga ggttctaaga tcagactgac aagtggtaac
    19981 agattcattg tgactagcca agacacaacg gaggcgagca acaagtgaac actatttaaa
    20041 actggttcaa taaaaacgat tccaattata caaataacgc taccagtcag tcctactagt
    20101 aggttgctga caagcatttc atatccagcc ttgaatttca catggaatct agaaacaaat
    20161 tgttcatata cgatgccggc aagcggcccg ataatcatcg ctcctacgat ttgtggagca
    20221 ttggaacgag cgatcaaccc taaagaagca attgcaccgg cgactgcccc tttttgtccc
    20281 cctaccgatt tcccgcctga ataactgatc aagatgggta aaagcagctg gatcatccaa
    20341 tggcatacct cttctaatgt ggtgtgcaaa tcgccagaca caccttttgt tgctaatgac
    20401 aagattcccc aagcgataaa cagacttaga tttggcatga tcatttcatt taagtgatgt
    20461 ccagcttttc tcagttgttt catagacacg cgactgctgt tgtttgcttt ccatttgtat
    20521 tccatctttt tcaccagact tctatttatt gatggacgga ttttatgtag aaaaagcagc
    20581 aaacgaaaaa ccgtctgctg ctttcagcta tctgggaact atttgcctgg ttcagacgca
    20641 atgattgcta gttcaccttg gaccgcccac tcagaaacgc catttggaat aatacagctt
    20701 gttccttttt ctaatggata tgtttttccg tcaacgacaa ggtctcctgc tccttcgatt
    20761 accgtcatca atgtataagg ggcttgtttt ttgaaagatg tgattccttt aatatcccac
    20821 tcatatacat tgaaaaactc agtttctaaa taagtgacga tcgatgaatt cccttttcgg
    20881 acttctttga tttgtaattc tggtgttttc gcagggacag ttgttacatc gatcgattgt
    20941 tggatatgca gctctcttgt ttgtccttgc gcatcttttc tgtcgtagtc atacacgcgg
    21001 taagtcgtgt cagaactttg ctgtgtttct aaaatcatga ttcctttacc aatagcatgg
    21061 atcgttccgc ttggtacata aaagaaatca ccttttttaa ctggtacttt cttcaaaagg
    21121 tcatcccagc gcccatcttt gatcatctca gctagttctt ctcttgtctt tgcatgatga
    21181 ccatagatga tttctgcacc aggttcagca tcgatgatat accagcattc agtttttcct
    21241 aattcacctt catgtttcat cccataggca tcatctggat gtacttgtac agatagatca
    21301 tcttctgcgt ctaagatctt gatcaataat ggaaacactg gttctgaagg atggccaaac
    21361 aattccttgt gttctgacca tagttcatct aatcttttcc ctttgaattc gccattttct
    21421 actacactga caccatgagg atgcgcactg atcgcccaat cttctccgat tttatcgtta
    21481 gggatatcaa aaccaaagac tgaacgtaaa cgacttcctc cccaaatttt ttcttgaaaa
    21541 actggtttta aaaacatagg ttcctgcata attcaaactc ctctcatttt tcatagctat
    21601 tatagcatta tttcggtaat accgctttct ctttcaaaaa gttttcaaca atttcatctc
    21661 gacatatgag atactccgca cgatgatcga caggatgtac gcgatttgac aaaaatatca
    21721 acgcttcttg tgtttttata tcaatcaaga aaaacgtacc tgtataacca gtatgaaaaa
    21781 gaatcgcatg accgtcttct acatcctttt ttaagtccca tcctaaagaa cgatagccat
    21841 tttttttcgg tgtttgatct tgcagtaaag aatcaatcgt ctcttcttct aaaaaacgac
    21901 cattacggat ttttcccttg ttcaaataca tttggacaaa agtaatgctg tctttcaaat
    21961 caataaagag acccgcgtta cctgcatgtt cagccaaaac tcttgctttc ggatcatgag
    22021 tttgtccacg caaaacctca cccgaaggca gttgttctgt gggaacgatt tttttggaag
    22081 gaaaaggaag aaataagctg ttcatcattc ctaatggttc caatacttgt tcttgaaaga
    22141 tttccactgc agtttcttgg taaatttcct ctagcatcaa cccaagaaga atcgtaccgg
    22201 catctgtata ttttacttct tttcctagtt gctcacctgg ttctagcttc aaataagcgg
    22261 cgatcaattc ttcctgagat agcttatctc tgtttggtat ccatgttttg atatcagaag
    22321 tatgtgtcag taaatgacgc aaagtgattg tctgattttc aaattcagga agatatttat
    22381 gaagcggttg atcgagttct atctttccat cttctaatag acgtaaaact aatgaagtgg
    22441 ttgcaacgac tttcgtcagt gaggctgcat caaacaacag atcttctgtc atcagctgct
    22501 tttcaggaac gacttgggca aagccggctg taaatgtttc tacttgattt tcttttatga
    22561 aggcgactgc tacacctgga taaaatcctt cttccatctt tttcaaaata agctgtttcg
    22621 tgtgtggata catctttttc tcctgctttt ctatcagatt cttacagtcc aagcttaaag
    22681 cttaatctat gaggaatcaa ccttttttac acaaaaaaac tgcgcgaaaa accgcgcagt
    22741 ttcccccggt atactatttt ctctttctat ctctagaata agcgatagta tgaccgttca
    22801 taaaatgacg agacatgcga caatcgttaa gaaagaaaaa gttaagcgtc gcatcgaatg
    22861 tcagttttat tcttttcttt tggctcactg acgaaccacc attcgattcc tatagcatca
    22921 tagattgtga gtactgtgtg ctttttgtct aagaagaaat caactgatag ttgctgcaag
    22981 tgagcaatca gtccttccat gctttcagta tcgatcatga atttcagaaa atcaagtcca
    23041 atcacttcat ctgtaggcaa atcaattgcg cctccgctag cttctgtcaa accaacatgg
    23101 aaatctcctt cattcaatac atggatgccc tcttcttcat tatgtacctt caaaccaagt
    23161 acatgattaa gaaattggcg ttcttttgtt aagttagtaa catttaaatg gattttttca
    23221 aaataagaac ctgttgatag tttactgaat ttttcttcag attttcttaa taatgcttct
    23281 tgatccattg tttctggaat ttctctttgg tcgttcttat tctcttcatg gtaaatttcc
    23341 aattcattct cttctgggtc tacgagcaaa atccccatac gtccatgatc ttctaaagct
    23401 tctttgattg gataattcat tttacggata cgacaaagga tatctgccat ttcttctgtg
    23461 ctagggataa tcaatgaaaa acggttcagc tttttgatca cccccatgtg atcattcgct
    23521 ctaggacttt cttccagcag caacatttca cttccagctt cttctgttcc taaaatggct
    23581 aattcgtttt cttctctttt caaatcaaag ccaacaacgt tcttgtaaaa atcaatcatt
    23641 ttatctcggt ctttcacccg aatggctaca ctttttaaat aggtggcagc ccccaattga
    23701 aaatctttca tatttcttgc tcccctctac gtcaagcttt attttaacga tggattcttc
    23761 ttctttcaag caataacttt gattttgtta aaaaaaatgt gagaaaattc gcattttgct
    23821 tgttttttgt aaattaatga gtaaaaactc ttataaaaag aggttgtgaa aaagctgttc
    23881 tgcatcaagt aataagaaca gccaaacaaa aatagctcct cctatttttc gtttggttgg
    23941 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaaaagtc
    24001 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa
    24061 tttggcttaa taccgagaaa ctggcttttg aacaccgttt accaagaggt tgtgagcctg
    24121 ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcagg tttgttgact
    24181 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga ggttgtaaaa
    24241 tcagcggttt gacctgagta ttagagatac aaaacggaaa aatagcttct catattttgc
    24301 cgttttttga gctaataccg aaggacagct gattgaacac cgtttattcg gaaaaaggga
    24361 ctgtgacacg acttttgtca cagcccctaa aaaaaataat aaatttgtac tattctatga
    24421 agcttcttct tcagaaaact gactgttata aagatctgca tagaatccgt ctttttccat
    24481 caatgtatcg tggtttcctg tttctacgat cgaaccatga ttcatcacaa taatgttgtc
    24541 tgcatcgcgt attgtagaca agcggtgagc aacaacgaaa cttgtccggt tttccaatag
    24601 gcggttcatc gctttttgaa tcagtatttc tgttcgtgta tctacactag atgttgcttc
    24661 atcaaggatc aggacatctg gatttgctaa gaatgcccgc gcaattgtga ttagctgacg
    24721 ctgcccttgc gaaatattgc ttgcatcctc gtttaataca gtgttatatc cttctggcaa
    24781 tttacgaaca aaatcgtcta cgtgagcagc tttagcagcc tcaatgattt gttcgtctgt
    24841 tgcatcgtcg ttcccatatt tgatattgtc atagatactt cctgtaaaca gccaagtatc
    24901 ttgcagtacc attgaaaact gtgcgcgcaa ttcatcacgt gaaagatcac gggtatctac
    24961 accatcgtaa cgaatactac ctccagaaac atcgtagaac cgttcaagaa ggttgatcag
    25021 tgtcgtcttc cctgcaccag tcggacctac aatagcgacc atctcaccag gtttgacatc
    25081 caagttaaaa tcagtcatta gcaagttatc tggtgtgtag ccgaattgaa cattttcaaa
    25141 acgaactttg tatggtgtat ctttttcttc tggaaggcca gatttttcat tggacatctc
    25201 ttcttcatcc aacacttcaa atacccgttc ggctgaagca actgttgatt ggatagtgtt
    25261 catcaaactt gcaagttgag tgattggttg agagaactgt gttgtatatt gcaagaacgc
    25321 ttgaacatcc cctaatgtca tgttcccatt tgcaactttt actccgccta gtacagcgac
    25381 gaacacatag cctagatttt taacgaaatt catcaacggc atgatgattg ccgaaataaa
    25441 ttgagctttc caaccagcgg catacagatt ctgattttct ttttcaaata cttcctggtc
    25501 tttttcagta tgattgaatg ttttaacaat ggtatgacca ccatatgttt cttctacttg
    25561 gttattcaat aaacctaaac ttttttgctg atctgcgaaa aacacttgtg atttcggcgc
    25621 tacgatcatc acgatgatca agcttaaagg gatcatcaat aacgcgacta acgtcaattg
    25681 ccagctgatt gaaagcatca tcacgatgac cccaatcaat gtgactgcac tagttacaaa
    25741 ttgagtcaaa ttttgctgca gcgtgcttgc gatgttatcc atatcattga ttgctcgaga
    25801 catgatatcc ccatttgtat gtgtatcata ataggaaatc ggtacacgat tcattttggc
    25861 ttccaaatca tgacgtaatt cataaactgt tcgttgggaa acacgtgtca taataaattg
    25921 ctgtaaaaag ttgaatacag cagaaatcaa atacatcacg attactgtcg caatgatctg
    25981 cgcgatttta tcaaagtcta tcggaaattt tgttacttgt tggccttgtt gcatctgttg
    26041 agcgccagcc attacacctt tgaatatttc tgtggttgct ttccccaaga cctttggtgt
    26101 ttggatttgg aagatcacgg ctgctattgc tagtacaaat acagcgatga tcgcaaccat
    26161 acgtttagac atatagccaa acaatcggcg taccgttccc cagaaatttt taggcttttc
    26221 accttttaca ccgagatttc tgccaggtcc accatgtcct ggaccgcctg ctggtccatg
    26281 agagggacgt gtgtttgttt tactcatgct aagtcctcct ctcttagttg tgaatgaacg
    26341 atttcttgat aagtttcgtt tgttgctaat agttcttcat gtgttccttt gccaaccagt
    26401 tttccttcat ctagcacaag gatcatatct gcatccatta ctgtacttac tcgttgagca
    26461 actaaaacgg tgatactttc tttcatattc gttttcaatg cttggcgaag attggcatcc
    26521 gttttgaaat ccaacgctga gaaggaatca tcaaacacat agatatctgc tgttttgact
    26581 aatgcccgtg caattgccag acgctgtctt tgtccaccag agaagtttcc tcctccttgt
    26641 tccacatggc tatctaagcc atcttccatc tctgaaacaa aatctttagc ttgtgcgatt
    26701 tctaatgctt tccaaatcat ttcatctgtt gcatccggct taccatactg catattgtct
    26761 cggatcgttc cagagaacag aactgctttt tgaggaacaa agcccattaa ttctcgcaaa
    26821 ttatattgag tcatgtcact aatgtccgtt ccatttattt ggatcgaacc agattcgatg
    26881 tcatataatc gagggatcaa attgactaag gtcgttttac ctgaaccagt cccccctatg
    26941 attgcgacga tctcgcccga cttggcttgg aaatcaatat cttccaatgc aagtttttct
    27001 gctccatgat atcgataatt gacatgattg aaggccaatg ttgcgttttt tccagcaaaa
    27061 gaaactgttt taggattttc agggtcatgg ataccgattt tttcatccaa cacttcatta
    27121 atacggtcag ctgaagcttg tgcacgaggc accatgataa aaatagcgga aagcatcata
    27181 aagctgatca agatctgcat cgcataagtc ataaatgcaa tcagattacc gacttccaaa
    27241 gtcatatctg ctatgtagtg tccaccaaac caagtgattg ctacgtttgt accgctcatg
    27301 atcaatgtca tcattggcat catcaatgcg acgatcgtat taactttgat tgccgtattt
    27361 gtataatctt tattcgctaa atcaaaacga ttttcttcaa agcgtgtctt atcgaatgct
    27421 cgaatcactc tgacccctgt caatccttct cgaaatacca ggtttaaacg gtcagttttc
    27481 ttttgcatac ttttgaataa tggaacagca aagtacatga ttccgccgat caaaataatc
    27541 attactggga tgacaactaa gaagattttt gtcaactgat gatctttttg ataagcaagt
    27601 atgctcgcgc caatcaacgt gattggtgca ttgatcatca tacgtaaaaa catttgtgta
    27661 accatttgga tttgagtcac atcattggtc gttcttgtga tcaaagaggc cgttccatat
    27721 ttatcaaacg catcttttgt cagactctct gattttttat agacatccgt acgcaattgc
    27781 ttacccagtt tctgtgattc acgagtagca aaaaatgtat ttgcgatagc agctaagata
    27841 ctgatcaaag aaaagccgat catcacaaaa cctgtatgcc aaatataatc gacatctcct
    27901 tgcgctaccc ctttatcaat aatattggag gtaagtgtcg gcaaatataa atctgccagt
    27961 acttggatca ccatgaaaac tgcagctgca attgctgaag tcaaggagat ccgttttacg
    28021 atttttagca tctagttccc ttctttctat ttatatactc cgtttttcag ccaagaaagt
    28081 ttcaaattga attcactgat tgcttgctct actgagtaat cttcagatgt tgaaaataag
    28141 cgatacgaat gcgcaattgt tgtaaataca gtattcatca ctaattggat cagtaattcc
    28201 aattcatgat cattttttac tttcagcttc gtccgatcga tcaattcaag caactctttt
    28261 gcatcttgtt tgtgttcttt atgtggatcg tgaggcgttt tttggcgact cgtatgagga
    28321 gcgacttttc ttgaggcacg gtaatccatg ttcataaaca agttacgata aaaagaagca
    28381 ttctcgcctt ccaaaatctc ataaatcatt ttagaaaaat aggattcgaa tcctttgaat
    28441 agatcaccat cggattcttt cattagttgt aacatatctt tagagctgtc acgtctcatt
    28501 gtctcaaagt aataaaaata aagatcctct ttatcttcaa aatactgata gaaactgcct
    28561 ctagggatat ccgctaattt gacgatatta gcaatcgaag cttcttttaa gggtactcta
    28621 gaaaactcga ttctcgcagc ctcgattagt cttttttgtt tttctttagg caaatggaaa
    28681 aatgtctgtg tcggcatatg gattctcctt tctttcaaga tgacacattg tcatatgacg
    28741 ctatgtcatt ataacacctc ctacctgttt tgcaagacat ttgcatagtt tttttcatta
    28801 tcatccgata cattaaaaaa gtgataagag gaatccttcc atttgaatta atgagagttg
    28861 cccattaaaa tagccacata ctaaacaaag gatgagataa atggcccaat caattagtgt
    28921 ccaagacttc aataaattgc ctctcgatgc tgacagcatc gttttagata caagaaatac
    28981 cttagattac acaaaaaacc acttaagcca ctcgctttct atccccgctg ctatcttgcc
    29041 aagacaatta aaaaatctag atccaaagaa actttattat attctaagct atacaggcag
    29101 gcgatcagaa gcattagccg atcaacttac tgctaatggt tttcgtgctg tttatatcat
    29161 tggtgggatg gaagctcttc agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag
    29221 aggctgtgac aaaaaatcgt gtcacagcct cttttatacc ctgtcttttt ttagttattt
    29281 attcttcaac cttatcaata tgacttgata gccttttctt tttcgagtaa actgattttg
    29341 ttagtcatag ttataacaat catagcaata aaaggagaag gtaagcatat ggcatctgtt
    29401 ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa ggcattgacg
    29461 tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt tttggaactt
    29521 tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga agcattacgt
    29581 gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc tcgtttcaat
    29641 gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc cttatggaat
    29701 ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc tggaaaaacg
    29761 ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc actgcacatc
    29821 caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt aaaaggtatt
    29881 ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat cgattatgac
    29941 cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc tttaggtaaa
    30001 tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat cttcaaagca
    30061 gcatttgtgc cttggatatt ttcccatctg tctctttttg ttattctttc tgctttttca
    30121 acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca tgagtaatca
    30181 ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt ccgaacaatt
    30241 tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac tgattcccta
    30301 gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa acgatcagta
    30361 ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga atcatcgcta
    30421 aaaatgatcc taagtaaggg atcaggttta aaaaacccgc aataatcccc aacgtgacag
    30481 catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca gcaaaagcca
    30541 cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc agcacattca
    30601 acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct aattgtttac
    30661 cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact aaaatagtcg
    30721 ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata tcttggatca
    30781 tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg cgaaattggt
    30841 tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct acatattgag
    30901 ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc actacgctcc
    30961 cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa agccttggta
    31021 cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag attcctgcta
    31081 aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac aaatatgaaa
    31141 cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag gctgtgacta
    31201 cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc ttattttttc
    31261 cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa tagcaggcag
    31321 tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac aagcatcaaa
    31381 aatcaatgat acgtgagcta ctgtctttaa attcccattg gccatcggac cgacataatc
    31441 gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag aaaaaggttc
    31501 gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg ctgttttatc
    31561 gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt ctgtaatctt
    31621 tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa cacattctca
    31681 ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat ttcattacaa
    31741 ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata cgcgtcgcga
    31801 aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag ccaacctttc
    31861 attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg gcaatcttta
    31921 cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg attttcattt
    31981 tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg atgaagcacg
    32041 tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc atatattagg
    32101 agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag gtcctcataa
    32161 aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac gattagtcgt
    32221 atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg gcaaattgaa
    32281 tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg tctttcatcc
    32341 aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg tgctttctta
    32401 taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc cagaagaatt
    32461 cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt tcctttatgc
    32521 ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg gttcccacat
    32581 tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg cactagatcc
    32641 aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat tatatcgccg
    32701 tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac cagaatgtgt
    32761 ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc aagacgtatt
    32821 agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg aagataaaga
    32881 gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact ttgaacaata
    32941 tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac atcaggacaa
    33001 gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga gatggttaga
    33061 ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt gttggttata
    33121 ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata ttacatttat
    33181 attagataac tctagttttt attgttatca aagatttctt ttattttttc aacaacaaat
    33241 tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt tttgcttcag
    33301 atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg atcatttctt
    33361 cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg cccattgctt
    33421 ttgctggtgg acgcaaagta ctgactttca taagcattgc ccgatatttt tttgttcctt
    33481 cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt gtttttcctt
    33541 catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg attgcttctg
    33601 tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt aacatgttcg
    33661 ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc ctcaaatgac
    33721 aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc gtgctcaaag
    33781 aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat caattgatat
    33841 tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca ctgtccgttt
    33901 tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa aagcagctga
    33961 taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa ataaaacgga
    34021 ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc gaacataaga
    34081 aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc tgaatagatc
    34141 cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt cttcaattct
    34201 tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata attctctaag
    34261 tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc atactttcca
    34321 tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt tttttcttcc
    34381 ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg attattctct
    34441 atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc taatgtctga
    34501 taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt cgtctggtat
    34561 gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg tacaagcatc
    34621 gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt caatgcttca
    34681 aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc actatatgtt
    34741 tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac gccgtttaat
    34801 cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga ctcttcctct
    34861 acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc tttgatcttt
    34921 tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta caaaaaccca
    34981 ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca tagtgccgtt
    35041 ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat ataccctagc
    35101 agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct tccttttttc
    35161 aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc agctaagatc
    35221 actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt gatcatcttc
    35281 aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt tgcaaaacct
    35341 aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc tgctagtgca
    35401 atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt ttccgtatgc
    35461 caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca ccactgcaaa
    35521 aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt tccttttaaa
    35581 atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt gctccttcat
    35641 ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc gaaagtaaag
    35701 aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct ttctggtttc
    35761 tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt cacttgattg
    35821 agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc attcttatgg
    35881 ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa tatctctgct
    35941 aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg gaaaaacatt
    36001 cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata atcttttaag
    36061 gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc ccctagatga
    36121 ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc agtcaagtat
    36181 ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac aatttcactt
    36241 cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc ctgatcgatc
    36301 aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga ggtagaaaga
    36361 actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat agaataaccc
    36421 aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat cgctccaaga
    36481 ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact tcttttgttt
    36541 tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact tgcttcgatt
    36601 atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc attagcattt
    36661 acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg
    36721 ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca ttcatctttc
    36781 actttcgtac tacgaataac tattccttcg gctggcaaat catctattat tttgaaatat
    36841 tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg aacgtgatac
    36901 ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac aactgtttac
    36961 catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag tgataaaaat
    37021 aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat tgagggtgag
    37081 atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa tttcaaaaaa
    37141 gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg ctttttcaca
    37201 acctcttttt atctaataac cactgtttcc aactcaacta attttgccca cgtttcaatt
    37261 tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc ctgaatccat
    37321 gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc aacaggtaaa
    37381 ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag tttataatgc
    37441 gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc aaatacaaga
    37501 cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct tggtttcgtc
    37561 gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc ttggccacta
    37621 gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc catgattttc
    37681 agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc gtatccttct
    37741 gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag gtcttcaaaa
    37801 ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc gatttcctgc
    37861 tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc atagtcgaag
    37921 gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga tacgcgatcg
    37981 attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa ttgaatctgt
    38041 gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc
    38101 acttttattc caaaactttc attataggct actgcaacat ccatccaatc agcaatctct
    38161 ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt atttaatcga
    38221 gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt catgaaatcc
    38281 atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg aagcaatggt
    38341 ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt atgcatccaa
    38401 gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt tgtgatcgta
    38461 tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc attttcattc
    38521 agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc ttccccatat
    38581 aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa cataattagc
    38641 cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg taaataatgt
    38701 ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct ggtaaataaa
    38761 ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg cgcatctttc
    38821 ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa gggatcaatt
    38881 ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta cccgctgtcg
    38941 atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc
    39001 gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag
    39061 tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc atgagtaggc
    39121 gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc cacaacgacc
    39181 atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac gaaatatcgt
    39241 gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc gtgtttaggc
    39301 aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt ctatcactcc
    39361 tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct
    39421 cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga ttccttggtc
    39481 ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc gatccatcat
    39541 ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt ccatcactgt
    39601 aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg ttttaaaaat
    39661 ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata tatccattcc
    39721 gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg ctaaatcaaa
    39781 acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg taatgttttc
    39841 accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt
    39901 gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag aattggtaaa
    39961 ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg tatgaaccat
    40021 gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga cattttctag
    40081 ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc
    40141 tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg gcggacaaac
    40201 cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg attcgcttaa
    40261 cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca agtcaatcgc
    40321 tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat ggtaggtttt
    40381 cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta ctttctcacc
    40441 agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt ctttcacatg
    40501 ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct gcggaatatt
    40561 gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat ttcgccaagt
    40621 acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt gcatcattgc
    40681 actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga atacttctgc
    40741 tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg aataggtcca
    40801 gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag gagtaaaaga
    40861 tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta aagatgtacg
    40921 accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga tttaactccc
    40981 tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg attattgtat
    41041 acttttgtac gtacatgtca agcagttatt tacaagaata caataaaaaa cacttgtttt
    41101 gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa
    41161 attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta
    41221 tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta tgaccggcgc
    41281 cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga taggatggat
    41341 cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc ttctttctga
    41401 taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg ctggttctgt
    41461 cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa ggattcttct
    41521 aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg aaatggctcc
    41581 tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt ctggcatgct
    41641 cctttcctat gttgcagatt atttgctaaa agggttgcct gaaacgatgg cttggcgcgt
    41701 gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt tagctctacc
    41761 agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa
    41821 ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa
    41881 acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat atcgttcctt
    41941 agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta atgctatttt
    42001 ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg atgcattgat
    42061 gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt tggttattgc
    42121 agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg gactttcttt
    42181 catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt tgttgattct
    42241 cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac tgacatgggt
    42301 aatcgtaggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc tagcctcttc
    42361 ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga ctgcttctat
    42421 gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg ttttatttat
    42481 tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc
    42541 taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt gtgacagaag
    42601 tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg aacaatttca
    42661 aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt aggagaacac
    42721 cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa aagctctcac
    42781 aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg tctcttattt
    42841 ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa tatttggctg
    42901 tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc
    42961 ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag aagaatcgtc
    43021 tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca acttatctag
    43081 cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt gttcttcttt
    43141 caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat
    43201 atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac ctttcatcct
    43261 gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat gtcctttttt
    43321 tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa tataagaggc
    43381 atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt ctctcaaagc
    43441 aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa cgatcaaacc
    43501 atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt gatcgttatt
    43561 cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga ttcctcggat
    43621 aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg tttttgttct
    43681 gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg accctttttc
    43741 tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg tatgtctgct
    43801 gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta cttcgtattc
    43861 ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg ccatttgcct
    43921 tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt attcaagcaa
    43981 aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat tctggaaatc
    44041 cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg tctaacggat
    44101 cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag cacccgtctt
    44161 ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttta gaagaaacga
    44221 atactggctc cgataattta tgccatgatt cttttaataa aagattcttg tgctcatcag
    44281 cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac gtaatgaata
    44341 ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc cgtttttccc
    44401 attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa ggattatcca
    44461 tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg gcccacacat
    44521 aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt tcccttttcg
    44581 tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc tgattttcaa
    44641 gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg cttgcagcaa
    44701 agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc aattcactca
    44761 tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct aattcattca
    44821 gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct gtaaaataat
    44881 aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg ataggattat
    44941 tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa ttacattcca
    45001 agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa aatagtttat
    45061 ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat cgctactggt
    45121 taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg ttatcttttg
    45181 ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat ctacgatcac
    45241 actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc aaatgatttg
    45301 ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat aataagttgg
    45361 attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt ttactaacaa
    45421 tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc
    45481 aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac cgacttcaaa
    45541 atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg ctagatacat
    45601 tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga tttcttcaat
    45661 ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac gctcattttt
    45721 aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca aagctaaata
    45781 atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt acgatggtaa
    45841 acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta acttcatgac
    45901 ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc ccacttgcca
    45961 tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct ttttttgccc
    46021 attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat attctactaa
    46081 attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg gtttcgcttg
    46141 tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg
    46201 ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat ccacccaatc
    46261 ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta attcctttac
    46321 ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc ccggttcata
    46381 aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca gccgagaact
    46441 gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt ccaacagctt
    46501 tccctacctt tcatttccta tagtcaagat aatagataca agtttccata cgccaccagc
    46561 aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta ataacaatac
    46621 gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt gttcaaaaat
    46681 ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata aaagaaacat
    46741 ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct gttcttctaa
    46801 taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca agttcttacc
    46861 aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc cctcaccttt
    46921 ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa
    46981 acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc
    47041 ataaagttta gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt
    47101 ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata
    47161 accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac
    47221 cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca
    47281 ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc
    47341 aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc
    47401 aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc
    47461 atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata
    47521 aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac
    47581 aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag
    47641 aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat
    47701 aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt
    47761 ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa
    47821 aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat gattttttta
    47881 ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag
    47941 gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc
    48001 tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata
    48061 aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg
    48121 gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt
    48181 taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg
    48241 tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat
    48301 agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac
    48361 cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga
    48421 aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat
    48481 tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct
    48541 ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc
    48601 gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat
    48661 acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt
    48721 aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa
    48781 agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc
    48841 gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa
    48901 tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac
    48961 taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta
    49021 ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc
    49081 ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat
    49141 ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac
    49201 aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc
    49261 attttcgata ctccattttt taccaatttc agttgtattc caatatttca aaaactcata
    49321 gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa
    49381 aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat
    49441 ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc
    49501 agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac
    49561 atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga
    49621 cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg
    49681 tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat cagttaattt
    49741 tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc
    49801 ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa
    49861 atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc
    49921 tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg
    49981 tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt
    50041 aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt
    50101 atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt
    50161 aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc
    50221 gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt
    50281 tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag
    50341 atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac
    50401 ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt
    50461 cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt
    50521 cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac
    50581 tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg
    50641 ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct
    50701 gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag
    50761 caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta
    50821 atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag
    50881 gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca
    50941 ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt
    51001 cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt
    51061 ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa
    51121 ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca
    51181 cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat
    51241 attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc
    51301 atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag
    51361 ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac
    51421 ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat
    51481 gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat
    51541 ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat
    51601 atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac
    51661 ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat
    51721 gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa
    51781 tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt
    51841 tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa
    51901 ccaaaatatt aataatgatt aaaacaaata atttaata
//
LOCUS       DACCZY010000005        50265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCZY010000005 DACCZY010000000
VERSION     DACCZY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.005"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..1401)
                     /locus_tag="IVZ67_000193"
     CDS             complement(37..1401)
                     /locus_tag="IVZ67_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8164298.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1398..2957)
                     /locus_tag="IVZ67_000194"
     CDS             complement(1398..2957)
                     /locus_tag="IVZ67_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8164299.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3013..5493)
                     /locus_tag="IVZ67_000195"
     CDS             complement(3013..5493)
                     /locus_tag="IVZ67_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164300.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(5477..5812)
                     /locus_tag="IVZ67_000196"
     CDS             complement(5477..5812)
                     /locus_tag="IVZ67_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164301.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5826..6860)
                     /locus_tag="IVZ67_000197"
     CDS             complement(5826..6860)
                     /locus_tag="IVZ67_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8164302.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6950..7630)
                     /locus_tag="IVZ67_000198"
     CDS             complement(6950..7630)
                     /locus_tag="IVZ67_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8164303.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7914..9017)
                     /locus_tag="IVZ67_000199"
     CDS             complement(7914..9017)
                     /locus_tag="IVZ67_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8164304.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9239..9709)
                     /locus_tag="IVZ67_000200"
     CDS             complement(9239..9709)
                     /locus_tag="IVZ67_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8164305.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10042..11682
                     /locus_tag="IVZ67_000201"
     CDS             10042..11682
                     /locus_tag="IVZ67_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8164306.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11836..13158
                     /locus_tag="IVZ67_000202"
     CDS             11836..13158
                     /locus_tag="IVZ67_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8164307.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13220..13651)
                     /locus_tag="IVZ67_000203"
     CDS             complement(13220..13651)
                     /locus_tag="IVZ67_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164308.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13715..14551)
                     /locus_tag="IVZ67_000204"
     CDS             complement(13715..14551)
                     /locus_tag="IVZ67_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8164309.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15293..15919)
                     /locus_tag="IVZ67_000205"
     CDS             complement(15293..15919)
                     /locus_tag="IVZ67_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164310.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16214..16549)
                     /locus_tag="IVZ67_000206"
     CDS             complement(16214..16549)
                     /locus_tag="IVZ67_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8164311.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16536..16820)
                     /locus_tag="IVZ67_000207"
     CDS             complement(16536..16820)
                     /locus_tag="IVZ67_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8164312.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(16876..18399)
                     /locus_tag="IVZ67_000208"
     CDS             complement(16876..18399)
                     /locus_tag="IVZ67_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8164313.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18392..19567)
                     /locus_tag="IVZ67_000209"
     CDS             complement(18392..19567)
                     /locus_tag="IVZ67_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8164314.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19571..19756)
                     /locus_tag="IVZ67_000210"
     CDS             complement(19571..19756)
                     /locus_tag="IVZ67_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164315.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(19722..21416)
                     /locus_tag="IVZ67_000211"
     CDS             complement(19722..21416)
                     /locus_tag="IVZ67_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8164316.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21413..21886)
                     /locus_tag="IVZ67_000212"
     CDS             complement(21413..21886)
                     /locus_tag="IVZ67_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8164317.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(21955..22110)
                     /locus_tag="IVZ67_000213"
     CDS             complement(21955..22110)
                     /locus_tag="IVZ67_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8164318.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22244..22624)
                     /locus_tag="IVZ67_000214"
     CDS             complement(22244..22624)
                     /locus_tag="IVZ67_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8164319.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(22628..22843)
                     /locus_tag="IVZ67_000215"
     CDS             complement(22628..22843)
                     /locus_tag="IVZ67_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164320.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(22846..23253)
                     /locus_tag="IVZ67_000216"
     CDS             complement(22846..23253)
                     /locus_tag="IVZ67_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8164321.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23519..24994)
                     /locus_tag="IVZ67_000217"
     CDS             complement(23519..24994)
                     /locus_tag="IVZ67_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8164322.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(24984..25832)
                     /locus_tag="IVZ67_000218"
     CDS             complement(24984..25832)
                     /locus_tag="IVZ67_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8164323.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(25869..26231)
                     /locus_tag="IVZ67_000219"
     CDS             complement(25869..26231)
                     /locus_tag="IVZ67_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164324.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26531..27155)
                     /locus_tag="IVZ67_000220"
                     /pseudo
     CDS             complement(26531..27155)
                     /locus_tag="IVZ67_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27152..27463)
                     /locus_tag="IVZ67_000221"
     CDS             complement(27152..27463)
                     /locus_tag="IVZ67_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164325.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27507..27800)
                     /locus_tag="IVZ67_000222"
     CDS             complement(27507..27800)
                     /locus_tag="IVZ67_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164326.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            27994..28641
                     /locus_tag="IVZ67_000223"
     CDS             27994..28641
                     /locus_tag="IVZ67_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8164327.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            28702..29847
                     /locus_tag="IVZ67_000224"
     CDS             28702..29847
                     /locus_tag="IVZ67_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8164328.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(29928..30233)
                     /locus_tag="IVZ67_000225"
     CDS             complement(29928..30233)
                     /locus_tag="IVZ67_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8164329.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30258..30689)
                     /gene="ruvX"
                     /locus_tag="IVZ67_000226"
     CDS             complement(30258..30689)
                     /gene="ruvX"
                     /locus_tag="IVZ67_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8164330.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(30686..30952)
                     /locus_tag="IVZ67_000227"
     CDS             complement(30686..30952)
                     /locus_tag="IVZ67_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8164331.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31207..31932)
                     /locus_tag="IVZ67_000228"
     CDS             complement(31207..31932)
                     /locus_tag="IVZ67_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8164332.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32293..32363)
                     /locus_tag="IVZ67_000229"
     tRNA            complement(32293..32363)
                     /locus_tag="IVZ67_000229"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32329..32331),aa:Gly,seq:ccc)
     gene            32480..33232
                     /locus_tag="IVZ67_000230"
     CDS             32480..33232
                     /locus_tag="IVZ67_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8164333.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33248..33727
                     /locus_tag="IVZ67_000231"
     CDS             33248..33727
                     /locus_tag="IVZ67_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8164334.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33978..35558)
                     /locus_tag="IVZ67_000232"
     CDS             complement(33978..35558)
                     /locus_tag="IVZ67_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8164335.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35551..36294)
                     /locus_tag="IVZ67_000233"
     CDS             complement(35551..36294)
                     /locus_tag="IVZ67_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164336.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36470..37246)
                     /locus_tag="IVZ67_000234"
     CDS             complement(36470..37246)
                     /locus_tag="IVZ67_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8164337.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37494..38330)
                     /locus_tag="IVZ67_000235"
     CDS             complement(37494..38330)
                     /locus_tag="IVZ67_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8164338.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38341..39270)
                     /locus_tag="IVZ67_000236"
     CDS             complement(38341..39270)
                     /locus_tag="IVZ67_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8164339.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39462..39815
                     /locus_tag="IVZ67_000237"
     CDS             39462..39815
                     /locus_tag="IVZ67_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8164340.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(39889..40545)
                     /locus_tag="IVZ67_000238"
     CDS             complement(39889..40545)
                     /locus_tag="IVZ67_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8164341.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            40771..42705
                     /locus_tag="IVZ67_000239"
     CDS             40771..42705
                     /locus_tag="IVZ67_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8164342.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(42791..44014)
                     /locus_tag="IVZ67_000240"
     CDS             complement(42791..44014)
                     /locus_tag="IVZ67_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8164343.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44008..45906)
                     /locus_tag="IVZ67_000241"
     CDS             complement(44008..45906)
                     /locus_tag="IVZ67_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8164344.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(45900..47018)
                     /locus_tag="IVZ67_000242"
     CDS             complement(45900..47018)
                     /locus_tag="IVZ67_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8164345.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47101..47934)
                     /locus_tag="IVZ67_000243"
     CDS             complement(47101..47934)
                     /locus_tag="IVZ67_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164346.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(47927..48844)
                     /locus_tag="IVZ67_000244"
     CDS             complement(47927..48844)
                     /locus_tag="IVZ67_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8164347.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49071..50195)
                     /locus_tag="IVZ67_000245"
     CDS             complement(49071..50195)
                     /locus_tag="IVZ67_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8164348.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        14962 a        10154 c         8489 g        16660 t
ORIGIN      
        1 attatatccc cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt
       61 gctatttgct ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga
      121 ggtgtattca gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg
      181 tttcacgata tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg
      241 tccgccaatt atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt
      301 ttcttcgtcg tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc
      361 caagaatggc gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac
      421 tgctgctgtt atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc
      481 tactgctctt gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt
      541 tttgtcaaaa ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg
      601 cagatattca ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa
      661 tgtcgtatta ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgtttgaga
      721 cagaagttgt acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt
      781 tggataaaaa tttatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt
      841 atcgaaatat cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt
      901 atttctcaaa gcatctgcta attccaatcc aatgtaacca gcaccgaaga tcactacttc
      961 ttttgcttct tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta
     1021 gacattttca gcttcaattt gtttatacaa ggtgacagga gaggaacctg tcgctacgat
     1081 taatttatca taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg
     1141 tttgtctgga tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg
     1201 aagagtccgc atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag
     1261 gttgattcca tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac
     1321 ttctgctttc ggttgaagtt ttcttagatt tagggcagct gtgatgcctg catgggaagc
     1381 accaatgatc acgattttca tgttttattc atccttaaat tcaatttttc taaataacat
     1441 tcttttaaaa attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca
     1501 aaaactttag cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca
     1561 tactgctttt ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc
     1621 tggatgattt ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt
     1681 tgcagacgta attctaactg cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat
     1741 aattcaaagc gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt
     1801 ctgaccaatc tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt
     1861 tttaatcctt cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga
     1921 ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag
     1981 aaatattctt ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg
     2041 tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta
     2101 ctgatcatga aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga
     2161 aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat
     2221 ccaacaggaa gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc
     2281 ataatggaaa gccatatttc aaatttcaat cgtccttctt ctgtaatagc aaaactaaac
     2341 tctttttgca gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt
     2401 tcaagatagg gacgtccaaa ccaaaataat tggaaataaa aataacgaat ttgactttct
     2461 ggaccggtca gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat
     2521 tccttcactc tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc
     2581 cgatcaaatg agccattttc caacagataa agaagtatct gatattttgt agaatagcgc
     2641 aagtaacaat gataataaac atcgactgct gctatatttc catgtctgat taataaaata
     2701 ttcccttctt ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa
     2761 aacagatctt ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg
     2821 gaaacctctc ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt
     2881 ttttcaagca aactgaaaag ttccaagtcc actccttcaa gcatttctga ttttaacagc
     2941 agaagtttcc ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca
     3001 aattctaatc acttagctgg ttggcccagc tgtcagtttc catgtgacca tgccttgata
     3061 cgttgcgcca ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc
     3121 gggaaagtca acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt
     3181 ttgaccttgc tgaatcaatt gctttgatcc agaatgattg aagtataatt ttgttccgct
     3241 taattcttga ccttgatcat tcttaaataa agaagtcaat gtacctgtca gttgccaagg
     3301 agacgtgctt ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgattcata
     3361 ataacttgtc ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt
     3421 aactaatgtt aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg
     3481 gttaggaagc ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt
     3541 cagtatgctg acggattgta aagaagtatc tacagtattt aattgatagt tgtaagtaag
     3601 atgaatggta tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat
     3661 gccggtatct gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt
     3721 ttcaggaagt gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt
     3781 gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc
     3841 aggttgtctg ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac
     3901 tgttatttga ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga
     3961 cggatacatg ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga
     4021 agccaatgca ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt
     4081 ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct
     4141 gtaatggatc gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga
     4201 tgcagaagaa aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt
     4261 tccgtctagc ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg
     4321 cgcatctttc ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg
     4381 agtatgagtc agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg
     4441 atcaaagcga ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt
     4501 ccgactattg gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc
     4561 agcaaatgta ttatattttc ctgttgaatc acttggtgag atgtaactat tccacgtatt
     4621 tatctctgca cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt
     4681 aggataaaat ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat
     4741 attattcgaa tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa
     4801 tttacccttg gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat
     4861 cttcgcgaca ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc
     4921 tcgcgtaata atagcaataa tatctgtcaa tagagtattt gtatcccacg accccatccc
     4981 aaaacgcaag gtatggcttc ctgctgataa taaacttccg ctagtaacct tgatgggggc
     5041 accggcaata tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga
     5101 tacatgttcc ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac
     5161 caaatcaagt acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc
     5221 tttccaagga tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt
     5281 agccccagtt agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg
     5341 tacgatattt tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc
     5401 ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga
     5461 gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc
     5521 ataacaacaa ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga
     5581 tcttttgttc attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta
     5641 gctgtgtctg ctgttttgat gcgccattga ctgaaccact tggatgcgga attggactta
     5701 gatcataaaa tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc
     5761 tgaagaacag caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca
     5821 cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc
     5881 aaccataaca ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct
     5941 tgtactgcaa aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag
     6001 ttttgtgtaa atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa
     6061 tcgccggctt ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt
     6121 gcaactaaaa atgcattaga cgtattttca atataaagct gattttcata aaagatcttg
     6181 ctcgtaaata tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca
     6241 gttggctttg cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc
     6301 gtctcttgac tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt
     6361 gctccttctg tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct
     6421 aatacacgtc ccttaaagct ttttgctggg attttcactt ctataggtac agttgtttct
     6481 gcatgtgctg atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac
     6541 ggcaatgtct catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc
     6601 gttaccgccg gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata
     6661 attacttcta gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct
     6721 ttatctattt ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt
     6781 gcttggctga ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac
     6841 agtcgtttac tttgattcat tgtccaccac atcctttcaa caaaaactgc ggtaaaaaat
     6901 ccttcaccta tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg
     6961 ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca
     7021 ctttcagtgt gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt
     7081 ttgctgctgt aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac
     7141 tagttggtcc agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta
     7201 attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt
     7261 tccacccttg tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag
     7321 gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg
     7381 taccaaatgg caattcggga actacatcca gtgttaaagg acctgtctgg ccagttgctg
     7441 gattttccgg atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcca
     7501 cggtatttgt tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa
     7561 aagctggtgt tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt
     7621 tctttttcat aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg
     7681 caaaaaatcc tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga
     7741 gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt ttttattaag tatgaaaaaa
     7801 tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa
     7861 aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttgt ttcttaaata
     7921 aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat
     7981 tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt
     8041 cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc tgcacctgat
     8101 gcgattgctt tgaaaacatg ctgtccgcgt cgtacaccgc tgtcaaacac gattggtaca
     8161 cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgctgctgg accaccatct
     8221 agttgacggc caccatgatt agatacccag attccgccag caccagattc taatgaacga
     8281 tagacatctt cttctgactg gacacctttc acatagactg gcaaatcaga atgagcagca
     8341 ataaattcta catcttttgg actaagcttc tgtttggaag atttgtacac agcatccatc
     8401 gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagtgg gaaggtaaac
     8461 ccatttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt
     8521 gcttttgctc cattacgctt ggccacttca agaatatcca aattgatccc atcatctttg
     8581 ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct
     8641 aatgtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt
     8701 gcagaagctt tctccgcctt cacatgagct aagccatgag cagcaactgg tgccatgatg
     8761 attggtgcag tcagcgtttc tttgtcaaaa tgtgtagttg tatcgggtaa ttctacatct
     8821 tttaatacat gaggaataat cagctgatga ttgaatgccc gttcattttc ttgataagta
     8881 aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa
     8941 gcggctattt ctaaatcttc catattgata aaatccacta ttccttcatt tgtacctgct
     9001 tgatacgttt tttccatttc aatgccttcc ctctataaaa aattgaatga ataatatgat
     9061 agcgtttaca cactttatat tactcttccg tattgataca ggcaaataat ctgtttacat
     9121 ttcagtcatt ccagttgtta ctactctgaa agaaatgttt agaacacaaa tagataaaaa
     9181 acgatgggac tttgacaaaa gtcgtgtcaa agtccctttt acgaaatact gtggatgcct
     9241 attgaaataa gtgcgggttg tttctttgta tttcaatcgt ttcttgccat tcttcgttta
     9301 attttggatt accgttttct tccttgaacg cataaggtcc caatccaaaa agctcagtta
     9361 gtatttgatt cacgattcca tatagctcgg tgtgatcgga cacatgatta ttgaagatca
     9421 tctcattatc accacgtttg aatctcacat tcgggctctc atgatgaacc tctatgcagt
     9481 tttccccttt ttttaactga cggatttctg aaaacatctc ataaatatct tctccaataa
     9541 aaccttcagc aagcgccaga ctgtccacaa gaccgcctac tgtcaactca gagattatcg
     9601 ctttgttggc accaagcttt aatgtatagc ttgttgcttc tactacgccg catgcattgc
     9661 aaccaccttc tgatagttcg ctttttctga tcaattgaac ttctttcatt ttctgttcct
     9721 cctcttagtt ttctacttat gtatttttta tcacctgacg taagtgtatt ttatcgtcac
     9781 gatcacgtga tttattcttc agagattcta tttatctatc taacatatcc tttattcttc
     9841 tacttttctt tctttagcat ataagagata cgcatagtaa tcaagcaagg attgttgata
     9901 taaaaagaac aaataccgct attttgattt cttttgactc tattactcat ataaataaaa
     9961 tttattatac ctgctctttt tgactatatc ctcatctgac tcctatactt cgtttgtatt
    10021 aaaacttagg aggaatcatt catgcttaca gggctaaact tattaaataa tccttttcta
    10081 aataaaggaa cagcttttac aaaagaagaa cgggcaaaat acggaatcac tggtatgctt
    10141 ccaagtactg tccaaacatt ggaacaacaa tctgtccaag cttacggtca atacttaagc
    10201 aaacagaccg atttggaaaa acgtatcttc ttaatgaatc tattcaatac gaaccggaca
    10261 ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt ttatgatcct
    10321 gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccaca agatgctgca
    10381 tttctttcaa tcgacgaacc agaaaatatc aaagcaagtt tgaaaaacgc agcagatgga
    10441 cgcgacattc gtttgatcgt tgtgacagat gcagaaggaa tcttaggtat gggtgactgg
    10501 ggtgtgaacg gtgttgacat cgctatcggt aaattgatgg tctacactgc tgcggctggt
    10561 atcaatcctg cacaagtctt gcctgtatca atcgacgctg gtactaataa cgaagaatta
    10621 ttgaataatc cattatactt agggaatcgt catgctcgtg tagaaggcga aacttactac
    10681 gaattcattg atcaattcgt tcaatcagct actgaattat tcccagaatt gttattacat
    10741 tgggaagatt ttggacgagg aaatgctgca acgatcttag aaaaatacga agataaaatc
    10801 actactttca atgatgatat ccaaggcaca ggaatcgttg tactagctgg tgtattaggt
    10861 ggattgaata tatcaggtga gcaattgaaa gatcaaacga tcttgacatt tggtgctggt
    10921 actgctggtg taggtatcgc aaacatcctt ttagatgaaa tgatccgtca aggtgtacct
    10981 gaagaagaag cacgccaaca tttctatcaa gtagataaac agggtttatt gtttgaagat
    11041 acaaaaggat tgactcctgg tcaaatccca ttcgctcgta aacgctcaga attcactaat
    11101 gctgatgaat tgacaaactt agaagcagtt gtaaaagcaa tccatccaac gatcatgatc
    11161 ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat ggcggctcat
    11221 accccacgtc caatcatcat gcctttgtct aatccaacaa aattggcaga agcaaaagca
    11281 aaagatttga ttgaatggac ggacggaaaa gctttagttg gaacaggtat ccctgctgct
    11341 gatgtggaat ataacggtgt aacttaccaa atcggtcaag caaataatgc gttgatgtac
    11401 ccaggtttag ggcttggttt gattgcttca acagctacac gagtaaatgc tgaaatcatt
    11461 tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg agcagctatc
    11521 ttgcctccag tagcaaaaat cactgaattc tctcaaacga ttgctgaaac tgtcgcaaaa
    11581 tcagttgttg cacaaaaatt gaatcgtgaa gaaatcacgg atatcaaaga agctgttgaa
    11641 tctgctaaat gggttcctga atataaatct ttagaagatt aagattgtta tagtaaaaga
    11701 acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca aaacagcagc
    11761 cagccaacta ctgctctttt gccgcttctc tttggctttt ttcatacgat acagaataag
    11821 aaaggatctg taaaaatggc acaaaaaaca acaaccgcaa ctgcagttga ggaaaagaaa
    11881 aaagaagggt tttggtcaac aaaaattacc ggcgtaagtt tgccccttta tttgatcatg
    11941 gtccttgtcc tgatcattgc aatggcttta ggaaaattac cgacgaatat gattggccca
    12001 atcgctgttt tggtcatttt cggtaattta tttcatttta tcggaacaaa aatccctatc
    12061 gtcaaaagtt atttaggtgg tggctccgtt tttgcgatct ttgcctctgc cgcgattgct
    12121 acatttggga tcttacctga gtatgtagta aaatccacag aaaactttgt cagcaatatg
    12181 ggctttttag acttttatat tgctgccttg attactggta gcatccttgg tatgaaccgt
    12241 aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc aatggtcgtc
    12301 tccttttttg ccgtaggact cgtaggtatg cttatcggaa atggttttgc aaattccgtt
    12361 ttatatatct cattgcccgc aatggctggt ggtgttggag ccggagctgt acctttatca
    12421 acgatttatg caaatgtttt aggtacggac gcttcttcta tcatttcccg cctgattcct
    12481 gcgacagcaa tgacaaatgt cctagctatc attggtgcag cgttagttgc tcgtgcggga
    12541 caatcaatgc caaaattcaa cggtaatggt aaattaatgg aaaaaggcga ccttggcgat
    12601 ggaaaaccta gaaatgtaaa accttccatt ccacaattag gtgtcggttt gatggtatca
    12661 ttgactttct tcatcttagg acaaatctgt aactattttg ttcctaaagt ccatgcttac
    12721 gcattcatga tcattatcgt tgttatctgt aaaatcgcaa acctattacc agaatattac
    12781 gaagatgcag ctatcatgtt caacaacttg attgtcaaaa acttgacagc agctgttctt
    12841 gccggtatcg gtattgcgtt attgaatcta aatgtattgg cttctgcttt gacatggcaa
    12901 tttgttgtct tatgtttgac aagtgttatc gcgatttcag tagtttcagg atttgtcggt
    12961 cgtttgtttg gtttatatcc aatcgaatca actattactg ctggcttatg caacaactcg
    13021 atgggtggta caggaaatgt agctgtctta tctgcagcta atcggatgga acttattgct
    13081 tttgctcaaa tgggcaatcg gcttggtggt gccatcgttt tgatcatctc aggtttcttg
    13141 atgcaattac tttcttagtc aaaaaaaata cctgatcatc aagtatcggc ttgatgacca
    13201 ggtatttttt gataaaaatt tacaggattt tcaaaaatct tttttgcatt ttatcggaat
    13261 gataaccgat gcgttcataa aatcggtgtg cttctactct gtattctgca gaattaagtc
    13321 ttattgctga tattcctcgt cttttcgctt cttctacgcc agtcatcaag gcctttccga
    13381 ttcctctttt ttcagcatgt ttagacacag caagtgccat aatattgaac atcggatcgc
    13441 tgaaaatcga ttcataggac tccgcttgta catagccagc tgctttttta gaagtaggat
    13501 cttcatagac caagaaaaaa tttttttctg gctccttcaa cagtttcttc atttgtctca
    13561 ccgtaacggt taatggtaca tcatatccta attgttccgc attgatttct cgtagtgctt
    13621 ctgcatctcc taaacgcatt tcccgcaaca tccaaatcac cagccaattt ttattttttt
    13681 aaatgaattt ttttatcaca gtatactcct tttttcattt agaacaatat gtttcagtga
    13741 ttttgacttg cgttagtcta gattctccta atgtttgtaa gatgacgaaa gcattgtaaa
    13801 gattgatcaa aagaaccacg aggacaatta cttgaaaaat aaaggtcact ttttttggag
    13861 acaaaaccct gctaaatctt gctccccaca aacctccaat aacagcagcg gggataacat
    13921 aaaacaacat agataaatca aagcgcataa agctacttgt taaagcaatc gttaccaatt
    13981 ttgccagttg agaaaagaaa atcgtagata gagaatatag tgttgcttct tttattggca
    14041 atgcaaacat cagcatcaac aaagaaacat tgactggtcc accaccaata cctaaaaaac
    14101 tagctaaaaa gcccagaacc cccccacata taaggtacca agtccatgac gtcaaatgaa
    14161 attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa
    14221 aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata
    14281 caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg acaatctgcc
    14341 aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac atcgtgaata
    14401 ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata agatcaagaa
    14461 tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg ttcgccagaa
    14521 cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt gtttatatct
    14581 tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa
    14641 agcttttcta ctttttaagg aaatttagtg ctaaacgaaa agaggttgtg aaaaagctgt
    14701 cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcataattt tcgtttggtt
    14761 gaacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg
    14821 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
    14881 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga gactgtgaag
    14941 tcaacgtttt gacctgatta ttagagaaac aaatggcaaa atagttcttc atatttcgcc
    15001 gtttttcgag cgaatgccga ggggcagctg attgaacatc gtttatttgg aaaaagggat
    15061 tgtgacaaga cgtttgtcac aatccctttt tttatatctt attaattcat tcttttgttc
    15121 tattcttctt cttccgccat gaatgagaaa cccatatcaa agaactataa aggttatcaa
    15181 aacagcgttt atgactaaat gaattagagt tatctatcac tatttttcaa gcgatttaaa
    15241 ccaaatggca tacaatatgg catacaaaaa cttcaataag ccgtgggggt ttttaaattt
    15301 cttcttgttc tttaaaatca atgagtggag ctacataatt tgaatccact ttttcaaacg
    15361 tcactttttg catcgacgaa tgttttcttt cccaagataa cgtaaataga tttgactctg
    15421 gacttataaa gtctacttca ccttctagta ttactttctt atgcaaatat tcctctaata
    15481 aatatagact tgctaaagca tacaacacat tttccaaatt tgcttcctta taatttgaag
    15541 aacgaccatg ttttactctg ttatatattt tccaccaatc tggtgtatct tcaggccacc
    15601 catctccaaa aggttttagt gaagtcaatg ttaaagaatt cataacccta acaccattat
    15661 ccaaacacat taattcgcta gatttaataa cagctttaaa atcagttatg cttttattgc
    15721 tactgctatc aaatccgcaa agttctttga aaataatctc acactccgaa ccaatcgcta
    15781 gtagtagttt ttgatattca atagagaatg ttttgaaatt attctcatcg agcatcacaa
    15841 aatctgtggt attaataaac tcattttcta aatgtagata atacttccaa taaaaatttg
    15901 taaactctgc tttgtccatt aaatcacctc ttttgaatta taacaaaaaa aacacgtcca
    15961 caacagacgt gttctttgat ttctacacaa tcagcttgcc aaaaccatcc aatcagtcct
    16021 agcatgcctg tattaaaatt gaacgttcat catctatcat ctttcttagt ccagcatgtt
    16081 tgcaaccaca cctacaattt tcagtccaac aatagatttc gactagctaa gtcttgcaaa
    16141 attcaggcag ctaacccttc atttactacc ttaattatac taaataccca caacgtttac
    16201 aagtgtaatc attctaagaa atctgtttag ctatgatcgt tgtataatct ttaaacgccg
    16261 ttccttcatt gattgaaata attttataac tcttgttttt ccaagctact ttcatatcgt
    16321 ttgttagttc ataatcttga tcgtatcgaa taataaacgt caacgttcct tcaagaatgg
    16381 tacctacact agcaagcacg tcttttagca tttgtgaacg cacattagcc caacattcaa
    16441 atacagtttc ttcactatcc accatctgcc catcttcatc ttttacccgt tttgtctttt
    16501 taaacttgac tctctcagtc aattcattta cattcttaat gattggcata ataagcacct
    16561 ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc gttcgctgct ggcttcttga
    16621 cggttcaaat accaatgttg agtaagtaat gacaccgcca aatcaaattg ctgataatta
    16681 tccatatctt tatccgttgc gctactatca atagcacttt ttatatattc gcctgctgta
    16741 tcaatcaatt gttgaataaa gtcatcatca atagtgtgat cgatccgcat actttttttt
    16801 atgctatcca aaattaccat tttatctctc ctaatgaaaa aaaggcagtg ggataaaccg
    16861 ccgcccaata tttttctata cttttggtgt tacaactggc gtaaactcaa tatacactga
    16921 agcattttga tcaattttct tataatcgtt gcgaacgatc actgcaagtc cttgtgagta
    16981 gtaatcgaat ttttcccatt gagtcgttac ttgatttcta cgagcaacaa agatagattg
    17041 tgcaatatcc cccataatca tagggaatgt tccagcttta ggattggcaa acaatgtatc
    17101 tgcaatcaat actactggca taccgaataa tgatttacca ctaggtgctg tcacatcggg
    17161 ttgtaaaatg taacgtccat ctgaatcttt caatgtatct aggtggtaat atccgctttg
    17221 gtttagaatt accattttat ttaatgctgg gtctaatgcc acattgtata gttgtttcaa
    17281 atcgtccaac gtagcagccg ttttcttagt gaatgttttt aataaatcca tgatatgctt
    17341 attgtcagtg ttttctacca atttcgctaa ctgatctttg acctcttgta caatattcac
    17401 tgctgaatct tccacaacct catttgataa ggcaatctta ccagcacgag tttctacttt
    17461 ataatcaact gaagtaaaca tttcagcgtc aatatcacca atttcagcaa gttcagcttt
    17521 tgttgctaac actgcttgtt ggttagttgc tactggatat ttaccttgtc catttgatac
    17581 tgtttttact gttgcatatt gagctaagtt ataatttgaa cgtttcaaat caaagacttc
    17641 accgattact tcttcgggaa caactgccgc tgcatttaca gtagtgactc catcacgcac
    17701 ttcgccttgt gaacggatat attcttcata cccacgcaat tctgtttttt cgttatcgat
    17761 taaagttttt tccatgttat tactctcctt ttgtgcttta gaagtggatt ctaagctatt
    17821 gtttgtttgt tttgcttggt tgttactcat aaacgattcg taagaacgct tatttacttg
    17881 gacatttgat gaatcataag cgggaacggt cacgacacta atttcattaa gtgctttgat
    17941 tttatctatt gatcgttcaa ttgtgccatc ttcttttttg tcgaatgaat ctaatccaag
    18001 cacgaaccca aaggacatag aatcaacaac cccttttgag atattctcat acacatcatt
    18061 tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat cctgtatcat cgacattcaa
    18121 tttcaacgta cctgctttga cactagctag tggctttgaa taatcgtgtc catataataa
    18181 gaacacattt gataaatcca cctcttttaa tgcttcgggt gtaatcactt ctacaaagcc
    18241 gcctaaatct tttgacggtt gcccgaattt caacgcatag cctgaaatag ttttgccgtc
    18301 tgttggttgt ttttcatttt ctttctctac tttgggagaa tcggctgtta gctcagcttc
    18361 ttctgtcagc cgtttttcct tttcttcttc attattcaac tggtgtaact ccttcctgtt
    18421 cttttaattg atatttctcc aacgtatcta aatacgtaaa gttcaaactt gctagtaaac
    18481 gatcgccacc atccatgggg ggtagcccca ttttcgaccg cccctcatta atcgtgagaa
    18541 gtgacccttt gacctgatcc aatacatttt ttacttttgt ttcaggatca gtctctagta
    18601 actgatcgga attaaagcgg atattggttt caagtttcgt atctaattca ctcacaaaca
    18661 cattaaagta atgaatcagc gtactttgga tatactggag attactttga acggtgctag
    18721 aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc aaacgccttg gcaatttgtt
    18781 ttgtgttcca atcattggaa ttgataagct tcaaaacatc ggtgtttatt tctaatgttt
    18841 tgtagtccat tgtttcatca agaatgatcg ttctaagagc attatctcca ctacttgaac
    18901 cgttggcttc ttcaaacttt tcacgaattg cgttctttgc tgatccgtct aaatctgact
    18961 tatgaacctt caaaatacct gacccactga caccacgagt aaagaaattg tgcaatgtgc
    19021 ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc ataaagtggt ggtaatcctg
    19081 ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc gctagacttc acacgtcttt
    19141 ttttatcacc aatctgataa gacaacgtac cattatctaa ttgagtaacc gttacacttg
    19201 agttaggtaa taagtgaatt tcttctacct tttcaccgtt gcgtttaatc tctgcaaagc
    19261 tattaccgtt tagcaacata ttgaccgcca aagcaaactt taaatgccaa ccatccattt
    19321 ctgaattagg tttctcgttt agtaacttca ccagttcatt atcagcttgt ggcatattgt
    19381 ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc aataattcga acggctgcaa
    19441 acacatcact atttcttaat gcacgaacgc ttgtaaagct ggtggtgtaa tcatccgatt
    19501 ggattgatac cacatgttgt aggaaaggat ctcccgttgt attttgtctc gctttaaaaa
    19561 atgccatctt ttaacctccc ttctgttctg tatttagcat gaccgctacc ccaataagca
    19621 ctatgcccag cgctaacaag ccatagtaga catttgttag taaggtgata gcaaccaaca
    19681 cacacaccaa tccaagaagt aaaagaacgg tatgaatgta attagaaaga aaattcttca
    19741 cttgtataaa attcattgtc tgctttcgct ccttccgttg tggtgaagta atccattgca
    19801 aacacatagg cattgattaa tgcagctatt ggatcaattt tgttactgtt ctttgattta
    19861 ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt tgttcactgc atgggtgaga
    19921 atcgtatttt tattgtgaat aacgttgcct tcataaactt gttctctaaa agttcgggtc
    19981 ggaacgttta gagtaatcgt tccttgtctt acttctaaca ttgggtagtt ggctttttca
    20041 gctttactga taagcgaatt agcgttatac gggtcataac aaatccccat acattccaaa
    20101 tcattttctt gaatcaaatc ttgaataaac tgaaatactt ggtcatagtc cacgattcca
    20161 ctttctaatt gagtaatgga acactcacca gctttttcta gttctcgata aggcaaaccg
    20221 tcgcgctttt ctttgtcttg aagcccatat ttggtggcta caaagctatg tgaatcacaa
    20281 taaagttcgc cgttatctag cggtacgatc catgaaacgc tagttaaatc atctgtttta
    20341 gataaatcca ctccgatata aacgggcttg cctgtaatat cttgtggttc tacttcggtt
    20401 gcctgccagt cttcagcaat catatagctg tcctcactcg cttgtctcca taaattgaag
    20461 tttttcacca atacagaatt aaggttattt tgtttcaaag caaggttcac atcatcttga
    20521 atggttggaa tcattacttt tttgatttct tcactttcaa aaatcggatt ggctttgatc
    20581 catttctctt gatcgtgaat ttcttcggga tcgtccaatt cccatatcgc tataaagtac
    20641 ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca ataagtattc ctcgtacata
    20701 ggaacgttta aatcaagccc agcggtacta ataacacaca ataagccgtt cttttgttgg
    20761 gtcattcctg acttaatgac gttgtacgtt ttacgagttt ttgcttcgtg ccactcgtca
    20821 agaattgcgg ttgttggtgc aaacccatct agcgtgctgg tttcactagc cagtgccata
    20881 gcgaacgaat tagagggcaa gtgctgaatc ttagagttca taattttcag ttgtggtctt
    20941 aaaaacttac tagacttgac cacgtttcta agtgaattag acagcatatc atagcccaat
    21001 ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc ttgcttctgc tggttcttgt
    21061 tccataatca acgcattggc agccatgcca cttgcgatat acgttttccc attcttacgg
    21121 ctcatactgg taaacgctcg attgaatcgc ctgtaattcc cttctttagt acgccagcca
    21181 taaagactgc ctagaatgaa tttttgaaat cctaacatct tgattgcttt accgtccgta
    21241 gatggtagca actcaatgaa tttaatcgct ttggtggctt gttcttcatc gaacacataa
    21301 gggaagctat ctgacttaga acgctccaaa tcgcttatat gacgctcaca cgcttggata
    21361 attttcttgg gtgcaatcag cttacctgat aggactttct caatgtaatc attcatgaat
    21421 tcatcaactc tttgaacggg tcttcgggtt catcattttt aaccttgttc aaagctagtt
    21481 ttgatcgtga ttctaaggtc atacctagtg aggtggcttg ttttttcaaa tcactcatag
    21541 cttgtgattg aacccgaacc gctggattgg ctttctttac tccgttttcg catgtgataa
    21601 acgtacctga ttttcttatc tcggcttcgg ctgttttcat tcgagcaaag gctaaacaat
    21661 aggctaccaa tgaaccataa tctgcttcac tcaatggata atcttttttt aatgttggta
    21721 cgatacgatt ccattcactt ctagcgctcg ctgccatcca gtcagggggc gtgatattta
    21781 ttaatggttg ctgcttgaac aattcttctc tagcgtccac acggattgct ttttcttcac
    21841 ttgatatatt gccttttgta tcgtgtaata ttttagggtg tcccatgttg tcctcctttc
    21901 agacaaaaga aaaaggaacg taagcaatta agctcacatt ccttcgtttt tcgatcagaa
    21961 tttttcttta tctaatttat ctatataaat gatttttccg ttctgcattt gaatggtcac
    22021 ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct gttttatcag taataacaat
    22081 tgtatttttc aataaatctc ctactttcat tatatacttt ttcacttgtg attacaagtg
    22141 taatcgttat ttgtaattac ccttatgcct tttcacaacg gttatttgta ccaaaatgag
    22201 taggcatcga ttgtcagtaa gtttcacact atgccccctt aactcaaagg gtactctttt
    22261 ctcgtctttg tctttcattg gctgtcttgg aattatggtg tgcatggcat aaagaacgaa
    22321 aattagtttc atctaaccgt ctattccaat catcttttag ttcaattttg tgatctacaa
    22381 catcagcttt ccttatcaag ccttcttcta ggcaatcctc acatactgga tgatttaacc
    22441 gatacaagta tgaagccttt cgccatgttc tactttgata gaattgaaaa tatttacctt
    22501 cgctttgctt tctttcttca cgcttaattg ccgtttgctt tgctttatgc ttcccacaat
    22561 acttctgcct ataatcaatc aatgttttac atccagcatg attacattgt tttttaactg
    22621 ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc atcttgttcc cattttgatt
    22681 tgtattcaat ataaggcgaa actatcttca agcacgttcc gtctttaaaa ttaactttag
    22741 tgattttatc ttcctcaaaa ctaataaagt ctacatcttc ttgagtgttt gaatactcaa
    22801 tgtgtttctc accatcgaag tatctaatac tttccatatg cataattatt ctacctccaa
    22861 taaattagtt atcaattcat catctgttag tagcaaagcc aagaacttat catggatgtt
    22921 taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt ctcgctcgat catataggtt
    22981 aagcagacaa ttaaattcat ccattagctc acctgtatca tcatagttgc cactatctac
    23041 caatgatccc actactagcg attgacacca cgctactgac tcctgcagcc aatcaggttc
    23101 ttcttccatt atctttaata cttctggatc aaatgtggaa taatcttctg ttaccaatcc
    23161 cacactaaat gccagcgtgt tcattagaaa ccgatccatt tcatctgtat attgaaagtt
    23221 cggtgctttg tttgttgaaa taatattgag catttacttt cctccttgat tcgtaacacg
    23281 tggtaacacc tacgtaacag gtacccgtta ccactcaatc ccaacgggga caagggttct
    23341 agcgttttgg taacaggtaa cgggtaaaat ccagtaacat tattccccag catattagct
    23401 aactatttca aaagttttaa ggaaataagg tgttaccccg ttaccaaatt gctgtatccc
    23461 ttggggcagt aagggtaaac tagtaacggg ctaggtgtta ctgacccgtt actacccgtt
    23521 actttttgta ctttccagct cgatttttat tttcataggc atttgactta ttgtaaaatc
    23581 ctcgtcgttt atttcttttt ggcagttgct tccgttccca accaggaata ctgctaataa
    23641 tcaaggcaat ctttttagtt tctgcatctg atcgaccatt caataaatcc tttgcttgtt
    23701 tatcaaaaac agcttccaaa atatcagcgg tgagtacgct atcaactaaa taaaactctt
    23761 gttgcatagt agctccatta tagaaatgct gtaaattttt ctcattacat ttactgtcag
    23821 gatagttctg ataataagaa cgtctcgccc acgctggagc ttcttcccat ttatacggga
    23881 tttccatttc caaatattta gtgattgctt ctttaattgg attctccatc ttggcatcct
    23941 cttgatagtc ttttgcaatt tctaagactt ctttatcttc atcgggacta aaatagattc
    24001 gttgtccttt atcaaaaagc acctttgctt cagctaacac ttgaagaaaa tattcatcct
    24061 ttgttttaaa cggattcatc tttggcttat tcttacaagg caaaggataa aaacggcggt
    24121 ttcctgtatc atctttcaaa tactgcccat cattcgacgt tccaataaat acacattgcc
    24181 ttgcccaagc tatgacattc cgaccataag gaggtctaat tttatcgatc ttggcactga
    24241 tgaagttttt tatctgttca attttagttt tgttcattga agataattct ccaagttcaa
    24301 taattacatt gccttgaagc tgcatataat catctttctt ttcacctagc gaatctaatg
    24361 aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac ggtactctta ccaatccctt
    24421 gcggtccatc caaaactgga acgatctcaa actttattcc tggtttatag atacgtgcga
    24481 ctgatccgac tatccaacgt tttgttactt ctcgtgtgta aaggttgtcg tctaccccta
    24541 aaaggtcaat aaacagtgtt tccagtctag gctgttgatc ccatttaact ttttcgatac
    24601 gttcttttac tgggttgaaa acattctttc tagccacatt tactactgct ttgtgtattt
    24661 tgttgtcatc aaaaactaca ttgtagtttt cttcaatata tgccgttaag gcatcctcaa
    24721 aactgtcttc ccattctcca gcggtacgat tgatccaagg agaatctttt aataggtgaa
    24781 tgtatccgct aaattcgtta taagcaattg ttccagctaa attgctatca ttggttaaaa
    24841 tcaattctgc attcacaaca ctgttacttt taatctgatc gtctttattg catcttaatc
    24901 gtgacatcca ggttttcaat tcaatgatat tgtcttcacg ctcgctggct tcgggaaagt
    24961 ctaaaatact atctaattta tgcttatgag ccatgagctt tccccctcaa atctttttta
    25021 atcatcgatc gaataatttg attcacttct ttatctttta gcggtggttc attgtacgca
    25081 tttgctgtat aaatcaattc tgctaaagtt tgattatcta caccgtgcca tagtaaccaa
    25141 ccgcataact tagttaaatg atcgtttcta ctaccttcgc cagcgccttg aacaatcata
    25201 tctaattttt ttgctgtccc tgtttttgct acgggtcggt attgagtctt tatttcttgc
    25261 ggttttttta gcagtaggtt cactagccaa tcgggagctg gtgcaatatt atttagagat
    25321 acgttttcaa taggttcata tgctctccca acacttcttg aaataatcat agttggcgct
    25381 attggaactc ctttagtgat aatatcaatc ccgcttaaac ttttaccttt atcatcacgc
    25441 ccgaagatat cagcaaatgc acctgtttct tgttggataa ttacaccatc aggaatttta
    25501 aagaagaaat ggattccttt acctggtgtg atttccgtat aagtagaagg aaatggatca
    25561 tacattgaag caagtttttt ataattctca acgccgtttc gtgtttcaga atgttgatct
    25621 aggtcaacta ccaccatttg atgatcttca agcattaacc caatatttct agttgcatct
    25681 tccgtccacc attcattaat ttgattagaa tcgttagttg catcattcca accactagag
    25741 ccttttattg gaattttagt tccaggagat aagggataaa ttgaccatcc cgacttttgt
    25801 aattccgttg ctgcttgata attatttgcc aataaacctc tcctcctttt tcaaaatact
    25861 ggtataaatc attcagacac ctccatgagc ttcaactgct caagttcgta tttcaaccca
    25921 tcccgaacaa attcagcacc ttttaattga ttctgatact ttacccatgt attaaatgtt
    25981 ttcacccttg cttcttgcat atcagtaatg tttaaccatg cggttaaaat ctgatttttt
    26041 agctcgttga tttctcgaca aattattttg tgttctgcat gttttttgag tacatacaaa
    26101 actgttagtt tttcattgaa tgaatttcca aagtaggttt tcccgatatt attttctagt
    26161 tcagcgatcc cttgattaat ttcctctaat tcaaggctat cgagttggtt taatagtttt
    26221 gttttgtaca tctacttatg ccctccagtt cttaattgat agtcgtactc gtcataagca
    26281 gtgaagagat ataacgctaa tcctgcaaag atagctattt ttgcaattaa tggtaataag
    26341 ccgaaaattc cgccgattaa aatgcttcct ataattaagt ttcgtttcat aatggttccc
    26401 tccgattttt taatatggta taatcggaac aaagaatatc gggttttatc ccaattaagt
    26461 tgcctttatc ctgtccgcca agactgttat ggataaggct ttttctttgt tctcgatcat
    26521 gttaatgctc ctaagttgtc tgcaattcag cagaacgatt ataaattttc tcactcactg
    26581 aaaactgcaa cccatatttt tctttaacag tcattaattc tactgtctca tctaaaatag
    26641 gttcacgatc aaccaacatt tcaggagtca tatctttctt ttttaccatt ttggaatgac
    26701 cataaatagt tgatacagct ttattggcta tcacattcgc tttaatgtag tctactggtt
    26761 ttggttcagt aatagcgtgg cttaatttag tcattgcttc tttttggtgt tctttgtcta
    26821 gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg gcgtagttct ttgatgatgt
    26881 cgaaaatcca gtcttgaaat gcctcggctt cttttttacg gcttttgaat gccatgcggt
    26941 aaatattttt tttcgttgat gatgtttaca tcttgtgttc tgcccaatga atcgatgacc
    27001 ttactagtag taaccccatc tttaggcaaa gagtgaatgg ctctagtaac ctgtttaaga
    27061 cctaacgctt ccgcaacatc tttcgccact gcccaccatt catcattaat atcgacaaat
    27121 cttataatgt gtccgttcca aatttcagtt ttcattcaac gtcctccagt tcaagcccta
    27181 gcaagctcgc taacaactca acttgctgat acaaaatttc ttgatactct tcaatagaaa
    27241 gtcgttcttt aaactctgct tgtactaaat cgttcaagcc acgcaatact ccgcttaccg
    27301 ttggttcttg tggtagcaat ttttgatagt ccatttgttc gtacaaacat tcaaacgttt
    27361 cttctagcgc tttgccttcc aagtttgcta attctgccgc tgtgtcttct gtaagaattg
    27421 ttgataatac tttttcgatt gctttatttt gtgtcgattc cattattatt tcctcctatt
    27481 aatccctttg cccccacggc ttgcgtttat ttttggtatt cttccataaa gcgatcacat
    27541 tcatcttgtg aaattcttac gactccatca actataatca actttaaacc gttaggaaca
    27601 tatttctgca tcatagtatt gtatgaaacg ttcatatact tagcagcttg tgccttgttc
    27661 atatattttg gaaactggta ctttttttct aataatgaaa gtaacgttcc ttgagtttgc
    27721 tctacttgtt gaacaactcg atcgtcaata attttaaaag ctggatcatc aagcgtgagt
    27781 ttgtcaattt ttaattccat atttatcccc cctcgttagc atacatttct atacaaagat
    27841 tttaaaaaaa tgcaagaggc tcttgaatac atttctatac ctcttataaa ctcaatatac
    27901 catcatacct tttcatttgc aagaatttta acatactttt ttgaactttt atgtttcaca
    27961 catatttaaa tcgtgatata gtagagaaaa tatatgaatg gtggtgaaaa tgtggctaga
    28021 aaaaaatacc ccgaagcagt ggagacagga caaagaatta gactggctag aattgaaaag
    28081 ggactctcaa tggaacaact tggaggtaaa ttatccccac cggccagtaa aggtgccgtt
    28141 agtaattggg aaaatggcta taatcttccc aataacacca ggcttaaaca actttcaaag
    28201 gtattagatg tatccactac ctatctatta aacggatctt atacattaag agatatgcat
    28261 atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat tgaaaaaaac tattgaaaaa
    28321 aataactcta taattgattc taatctttct aatgctatca ataacatcga agaagataac
    28381 ctgactgtct cacagaagat ttatttaaca aacgcaataa aatatcttaa aacaggatct
    28441 cgaattgaat ttcctgacgg gagtacccca attgcttatc tcgcagctct tctaaatacg
    28501 cttttacgat atgactcatt aattaatgat tcagaaaaaa atacagatga gaaaaaagag
    28561 tatcaagaat tttatgaaaa tgatcttcta aaagagttca cagctcttgt tactgagcta
    28621 aataaacact attcaaaata ataataaact gcctcctcca accgccccca cggcttgcta
    28681 cactatggag ggaaaactat tatggcaaca ttcaagcaat atgaaactaa aaaaggtaaa
    28741 ttttggctct atgaagccta tcttggaatg aacaaaatga ctggcaaacc tgataaggtt
    28801 cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg ccttgtcacg cttgcaagtg
    28861 gactacgata aaaacggtat taaaaaagcg aacaacgaaa cgtttaaaga agtttatgac
    28921 ttgtggctag aaacatataa aaccactgtc cgagaagcca ccttcatgaa aactgaaatc
    28981 aagtttagaa agtggattct tccaaaatat ggaaatcttc gtgtgaatga agtcacggta
    29041 aaaaaagccc aacaaattgt aaatagctgg gctgaaacta ctgaccaata caaggtactt
    29101 cattcaactt ctaagcgtat ttttagctac gctatcaatc ttgggattat tgagactaat
    29161 ccactagaca acgtattaat gcctaaaaga accaccacta agcataaaga aaaggtcaaa
    29221 gtttacagta aagaacaact gatcaccttg tttgattacc taaacagcaa acctgaaact
    29281 tatcgaaacg actttgataa aacgttacta cgctttctct tttatagcgg ggttaggatc
    29341 agtgaagccc ttgcattgaa ttggtcagac attgattttc aagaaaatac ggtcaccatc
    29401 aataaaacac taagccaatc caaaaatggc tataaaatca gtgatcctaa aactgattgt
    29461 agtgctgggg ttcttccact agatgaaaga acaatttcta tattaaaaaa atggcagatt
    29521 aatcagcgga aatatatgct gacacttggt gtaactgatc cgactatgat tttttgtggt
    29581 atctataaag aactaatcac acaccacgct atttatgcaa gattgattac tatttgtgaa
    29641 aaagctatgg ttccatttct cggtgtgcat gtagcaagac acactcatgc ttcaatgctc
    29701 ttggacgctg gagctactat ggtagaagtt caaactagat tacggcatac aaaaattagt
    29761 cagacgatcg attgttacgg tcacttggca aaagaaacga aagaaaagac tgttgaaaaa
    29821 ttagtacaac acttaaatat tgactagatg gcatacaata tggcatacag taaaaaataa
    29881 aaaaaggaga aaccctatca tatcaaggtt tctcctagct ttatagtcta ttcttcttct
    29941 tccgccatga atgtattgaa tacttcttca atcatgtccc attctgcgtc tgtttcgatt
    30001 tgttctaact cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct
    30061 acatcttcat cttctgaaac accagcagga tataaaagaa catagttacg tccaaattct
    30121 tcttgtccgt caactgttag taggatttca tataatgttt catttccttg gtcatccacc
    30181 aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt tatgttctgt cataagatta
    30241 cctcttttct tttattatta aaataattgc ttacctgagc cgtctaaata attttgtaag
    30301 atcatcacag ctgctaattt atcgatgact ttttttcgtt tggctcttga agtatttgcc
    30361 tgctctacta gcatacgttc tgcctgaaca gtggttaatc gttcatcttg ataatcaact
    30421 ggcaaggaga atagttcttt gatcttttct ccgtatgaca tggaagcttc tgcccttggt
    30481 ccgatggaat tgttcatatt tttaggtaat ccaactacaa accggtctac ttcatattgt
    30541 gctactaatt ccttcaaccg atcaaagcca aactcttctt ctgcttcatt gatccgaatg
    30601 atctctactc cttgagctgt ccagccaaag gggtcactga tagcaacacc tactgtctta
    30661 gaaccgacat ccaagcccat tactctcata aattcactcc atgatttgag aggtagtcct
    30721 tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg aatcaaattt cgagcatcac
    30781 gataacgagg aatgtaggac gggtctccag aaagcaagta gccgacaatc tgattgattg
    30841 gattgtagcc tttttcttcc aaagcccgat aaacaatcgc taatgtttcg ctcacttctt
    30901 ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc atgattctac
    30961 acctctttcc cgcaaggtaa ttccagctta ccttctacaa ccttatttta ctcgaagaaa
    31021 gggaaaacta caaacaaata ttccaaactt ccgaataaat taagaaacac tatttgtatg
    31081 tttttttcct tttttctgta gctttatcaa aagtactctt ttcataaagt atacgctata
    31141 tttctttgaa tgtatgtttt tacacaattt tttcataata ttcataaaaa attcgaatag
    31201 ctgctactaa ttttttgttt ttgttgcttg tcctgtctca taattgatcg tgtagccatc
    31261 ttgaacattg tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc
    31321 caattgcact cccctggcta ccagttcatt cccctcaaaa tgaggagtga cacggtagcg
    31381 tacatgatga tttgtttctt tgatatatgc cgcaatatca ttttcgtgag caagcatgta
    31441 tggagcattc aacgaacgtg tccctgtcat caagttttta atgttgttat tttgaccagt
    31501 cagctggaaa ccaattaaat ggctgcgatt atataaccat tgcccgtcac ttaatttctt
    31561 ttgtttccaa cctgtcgggt caatatacaa aggctctctc ttttcagtag ggaacaaatc
    31621 ttttccaagc atagcattgg cttgtcctac tctgttcaat ttatcaatat ttgaatagct
    31681 ttcccatgtt cctttggaaa ggctaagatc ctcttttgta aattccggct gattcccatt
    31741 gatttctatt tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg
    31801 tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata tctgaatctt tttgtgtggt
    31861 ggaaacaccg aatgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt
    31921 ttttttgctc atttctttac tcctttacta acagctccct aaatcttaac atatgttccc
    31981 ctataaataa atactagaaa gaaatagaaa cagcaaaaaa taaaacctgt tttaagatgg
    32041 cttaaaaata caaaaaagag acggattcct cgtctctaat cagtcaccac tctatctgta
    32101 aggtatttat tattattcaa aaattttctt catactcaag acaatacact gatttcacaa
    32161 ccgcttggta aacggtgcgc aaaagcttgc atctgcggta ataagtccat ccaagttaaa
    32221 aacataagaa gttgtttttg cttggctgtt cttattactt gttgcagagc agctttttca
    32281 caacctattt taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga
    32341 ttttaccact aaactacacc cgcaatacaa aaacagtata cgcctctttt attccttcct
    32401 caagtttttt tattctttac gcttctggta tgttttgtag taaaataaaa ctaatcaatc
    32461 aattattgga gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt
    32521 actcgctggg aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat
    32581 gagtcgaatt gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg
    32641 tctctttatt ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc
    32701 gattaattta gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga
    32761 gctaacatta gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt
    32821 tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt
    32881 tggcggaaag atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc
    32941 ttatctatat agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt
    33001 attgattggc atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct
    33061 aatcattatc ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg
    33121 tgatttgcta gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac
    33181 tgctacgatg atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa
    33241 cacaagtatg gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt
    33301 tgccgttatt acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt
    33361 tggctggatg atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt
    33421 gcttggttct ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc
    33481 cgtaacgatt ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca
    33541 agcggttcga aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc
    33601 aattatgatt gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa
    33661 catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt
    33721 taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg
    33781 acaaaatatc tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga
    33841 gtattaaaaa aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata
    33901 ctgagaaact gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt
    33961 gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc
    34021 catagagcgg aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga
    34081 taaaacaaag ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc
    34141 atcatagttg cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg
    34201 gtccagttca gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag
    34261 cagccgaata aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac
    34321 agtccgctta ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg
    34381 aacttttctt ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt
    34441 tcttgatcca aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc
    34501 aatgcgatcc ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt
    34561 atttcgcctc ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa
    34621 agaacttgca taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc
    34681 tgatgcacat tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca
    34741 gtcagttgct ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga
    34801 gccgtgatag ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca
    34861 gcatcataga atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata
    34921 gtccccgttt gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca
    34981 agcaagatac tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca
    35041 aaaacaatca aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt
    35101 gagataatga tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg
    35161 actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa
    35221 aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa
    35281 aagatattat aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg
    35341 taataagtaa agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa
    35401 atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc
    35461 tttccctttt ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga
    35521 ttaactcgaa ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg
    35581 ccttccagcc attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa
    35641 ttcactaact gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca
    35701 taactgttgt gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc
    35761 atgagccttc atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg
    35821 ttcatccaat acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg
    35881 acgcattcca tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag
    35941 cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga
    36001 aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac
    36061 ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa
    36121 aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc
    36181 ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc
    36241 ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt
    36301 actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca
    36361 aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt
    36421 ctacaggaaa acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt
    36481 cattttctat cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct
    36541 ctagttgttc tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt
    36601 tttcagtatt aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt
    36661 ttttatattt tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta
    36721 ttttattttt ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt
    36781 gatcgctatt accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat
    36841 agttttcttt ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga
    36901 attcttttat caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat
    36961 tcggatgaga cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc
    37021 ctagttcgat accgctataa taggcgttca aatcgatgtc taggaaaaaa atatctgagg
    37081 aatagatttc attattttta atgaattcaa caaattgtat aacgttcaat ccctgaacga
    37141 tctcaaattt ccaaggactt aataaaattc tggattcttc caaaatttct actagcattt
    37201 tttcaaaaac caaactatca tccaaaataa atatacgcgt taccataaaa agtacctcca
    37261 ctcgatattt tactatcaaa atcatttcat acaacttttc aacatacatg ttaggaataa
    37321 attgtaattc tcttcacaat cttttccaac acctttgaaa ccgatatcaa atactttcag
    37381 gatgctgctt ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa aagccagaca
    37441 accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt cttttaaaaa
    37501 gaacgataca catactgatt tttcggagca gagactttct cagcgtttgt aacaactaca
    37561 gatgggattt cacgcttgaa tggttgataa tagtctgatt ggatggttcc ttctatttta
    37621 taccattcat catttttaac cgttactcct tccggcatat gcgtcaacaa accaaagact
    37681 cctgaatctg ccacacaatg gataatgccg aatcggaaca agaagatatc tgtattgcta
    37741 tcgttcggtg tttggtaaac aaatccttca tagctgattt ttttgccaat gaattcgcta
    37801 gggtaattat aaattagctc cataacttct aaatagtttt catctgtgat cttgatagtt
    37861 tgcttatccc catacttttc tttcaactca ttcatttggt ctaagtagtc tgatttgttg
    37921 aaataaatac tggtatccgg ctttaagtat tgtgtctgca tctccggatc ccctactgat
    37981 tctttactta ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc gagactaact
    38041 gttggaaaca acgtccccac gattactggc aaagctagca gtacataagc gattccacgt
    38101 tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc ttctgctttt
    38161 ccgtttttga cccacaaata cagctgaact aaagccaaaa gagcagataa aatcattgaa
    38221 agaatagcta agtagcgata atggacattg atatattgat cgagtttgcc agaaacttgc
    38281 aagtacatca tcaattcgaa atacccagat aaaatcaaaa accgcagcat attttccctc
    38341 ctaaataaat aatgtgaatc caataacgct aagggcaatc agtccgatca gcgataaaat
    38401 aaagcgtcct gtaaaatatc gtttcatcat cagtagattt ttgatatcga ccattgggcc
    38461 aaagaccaaa aaggcagcta caggtgctgt gccgaacaga cttaacaaag aagatcctat
    38521 aaacgcatct gcttctgagc aaagagacat cgtaaaggcc aataacagca tgatgataat
    38581 ggctaaaatc tttgttgagc cgagcgttag cataattcta gttggtaaat aagtctgcat
    38641 cgcagctgcg attaatccgc caatgatcaa ataacgacct gtatcaaaaa attcgtcgat
    38701 tgaatgtgtc aacacatgcc cagtttgatg gatcagctga ttttttggtt cattcttctc
    38761 actttgttct tcttcatgga catgatgatg gtgattttct gctactttag acgtgatttt
    38821 ttctttcaat acagcgtctt tttgaaaata ggcaagccag acaccaatca caagggcaac
    38881 aaaaaagctg cctatcaccc gccagcttgc catctgccaa gaattgccaa atgcgataaa
    38941 agtggaaaaa atcacgattg gattgatgat cggagcagtc agcataaaag caaacgctgt
    39001 gtgaactggt acgccttttt taacaaattg atgaacgatc ggtacgatgc cgcattcaca
    39061 tgaaggaaag aaaaatccta agatcgaacc agtaaaaata gagaagatac gattttcagg
    39121 aagccatctc ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg agataagaca
    39181 gcctaataat acgaaaggta atgcttcaat aacgatcgat aagaaaatcg tactcatctg
    39241 caaaaccgaa tttggaataa aagaaaacat gagtttcctc ctttttattt ctagttcatt
    39301 cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca
    39361 actcttattt tttaccaaag cacttcaatt gactttttct gttattccca ttaaattaag
    39421 gtctcataca tatcacttct atcataaaag ggagaataaa aatgaaacaa accactaact
    39481 tgactgaagt tcaagacata ctgcaacagt catccgttgc cgtcatttat ctttcgatgc
    39541 ctaattgcag tgtttgtcac gctgttcgtc cacgactaga ggaacttctc acacattatg
    39601 atattccggc acttcatttg gatgcatttg aaacaccaga agtagctagt aaatttgaag
    39661 tgctgactgc gccagttgtc ctgatttttc atcaaggaaa agaagtcttt cggcaagcac
    39721 ggttcatcga ctttaaaaaa atccgttatc tactagatga actgacagca gaagatgatt
    39781 ccctttctta tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga
    39841 ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct ttttattttt attctgctgt
    39901 attttcggaa tgcgcatttg cgtcagaatg caagaaatag atcaaggttt gcaattcatt
    39961 cgtcaaatca acattttgga tcaatacatc tgacggagca ttcaatcgag cagcggtaaa
    40021 gttcaagatt cctttgattc ctgcttccgt taactgattg actacttctt gtgctctttc
    40081 agcgggtatc gttaaaattg ctacttcgat ttgttggatc ttgatttgtt ctgacatatc
    40141 ttccattgga taaacgggga taccgtctac gatgcgccca acgatttcct cattcacatc
    40201 aaaagcacaa ctgatacgta tgctattact ttgatggaat ttgtatttta gtaaagcact
    40261 tcccaagttc cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt
    40321 tgcaaaaaag tgcataagat cttctacatc ataaccatac ccacgttttc ctaattcgcc
    40381 aaaataagaa aaatctcgac ggatcgttgc actatctact tgaacggctt cacttaattc
    40441 agttgaagac acttttttct ttccagcatt atttaaaatc cttaaatagc gataatacaa
    40501 aggcagtctt ttagctgtcg cctttggaat aatatgatct ttcacgaaat gacctccacg
    40561 tattgtctaa ttacatcttt gatttctcca tcatagcagt atttatagga aaaaatcaat
    40621 tccattgctc agatattgtg aaattttatt ctttattcat ttatttatca tttctgcaaa
    40681 tctcgcagta taatggaatt tatggtagac tactaattga aaaatagttt tttatttgag
    40741 gagaattcac aaagtagaaa agaggattcc atgatattat tacaagccaa tcagattgct
    40801 cgccattttg gtgcagaagt cttatttgaa aacatacatt tagaaatcag tacaggcgct
    40861 cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga ctttattaaa gatcattgct
    40921 ggaattgaag cacctgatga aggaaagatc gctaaaaata aaacagcgac tctcggatac
    40981 ctcgctcagg acacaggact tgattccaat gaaacagttt ggaacgagat gctgaaagct
    41041 tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc tagagatttt cattagcgaa
    41101 ggtacgccag acacacctgc ttatagttct tatttaaaag aatacgaccg gctgcaacac
    41161 gatttttcag atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt attgcatggg
    41221 tttaaattcg attcttcatt ttatgatcag aaaatcgata ctttatcagg cggacagcgc
    41281 acacgtttag ctttagctcg catgcttttg caacaccctg acattttgat tctagatgaa
    41341 ccaacgaacc atttagatat cgagacactt tcctggcttg agaattatct gcaaagctat
    41401 actggtgcat tattgattgt ttcccatgac cgttattttt tagataaagt agtcaatgaa
    41461 gtctatgaat taagcagaag aaaaatgcgc cattacaaag gtaactattc gaaatatcta
    41521 gatttgaaag cagaacagtt agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa
    41581 atccataaat tagaagactt cgttgctcgg aaccttgttc gcgcctctac cacaaaacga
    41641 gcgcaaagcc gcagaaagac attagaaaaa atggaccggc tggatcgtcc tcaaggggat
    41701 gaaaaacaag caaactttct attcagtata gaaaagcctt ctggaaatgt agtcttgcaa
    41761 atcgaagatg ccgctatcgg atatgataat gtcaccttat ctgaaccaat caatctagat
    41821 atccgtcgtc aagaagcgat tgctctagta ggtccgaatg gtatcgggaa atcgacgttg
    41881 ctgaaatcaa tcattcacca gatccctttt atccgtggaa aagaaacgct agggacaaat
    41941 gtcaccatcg gctattacga tcaaggtcaa gctgatctgc atacaaacaa gacgatccta
    42001 gcagaattgt gggatgagca tcctaccact ccagaaaaag agatccgaag tgctttaggt
    42061 gctttcttat tttctggcga agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa
    42121 aaagcacggg ttgctttagc caagttagcg atgaacaaag aaaatttttt gattttggat
    42181 gaaccaacaa accatttaga tattgacaac aaagaagtat tagaaaatgc attgatcgat
    42241 tatgacggca cactgctttt tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca
    42301 aaagtcgtcg aactctctga aaatggcagc aaagtctatc taggagacta tgattattat
    42361 ttagagaaaa agcaggaaga agaagaaatc gccgaattgt tggcacaaga tgagccagaa
    42421 tctgctcctg ctcccacgaa caaatctgat tattaccaaa gcaaagaaca gcaaaagctt
    42481 atccgtagcc ttcaacgaaa aattactcag gttgaagaag aaatggcgcg gatcgatgaa
    42541 tggatcgatc agttggaaca agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa
    42601 ctcaatgagc tgaaccaaga tttagaagct gctagacaag atcaagaaac aaaattgacg
    42661 gaatgggaag aactaagctt ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac
    42721 agaaaccatt catgctgttt tggtttttct ttttttattt tatcaaacaa aaattccata
    42781 tttacctttt tcaccaataa gaagaccaat tactagtcat tctcactaaa gcttctaaat
    42841 aatagtaatc accccaaata caaggtgtat ttaccccatt atttttgggc ttattataaa
    42901 cagactgtgt taaaaagcca tcttcttctt ccgttgtagc atatctttct ttcaattgtc
    42961 gcataatttt ccaaacagca gactggtata agttcttttc tgttttgtct aatagacctg
    43021 attctattaa ttctaataat ccacaaacca tgatcaccat actagatgta tctttttctt
    43081 ctctgctaga atcatcaaag atcaaatccc aataacaaat ggtatcttct ggcaaccgat
    43141 tcataaaata attggctaat attttgctta aatcaaaata tttttgttct ttcaaataac
    43201 gataacttaa tgcaaaaccg taaatccccc atgcttggcc tctagcccaa caggaattat
    43261 cactataacc ttgatgcgta cttccatatc ttggttttcc atctttgaca tccatataaa
    43321 atgtatgata agttgaacca tcttctctta ctaaataatc tgctgcattt tgaatatgag
    43381 cctgagcagc ttttttatat ttttgatttc cagtttcttc actcgcccaa tacaataatg
    43441 gaagattcat gcaacaatcc atgatcattc taccttgttg tgaaggatca ctaggatctc
    43501 cccaagcttg tataactcct gccttctccc aaaaacgctg aagtaatgtt tctgcagcat
    43561 caattgaagc ttccttcatt tttcgatttc ccgttatttt ataatctgct acggttgata
    43621 aagaatacaa aaaaccaaga tcgtgatgat tagtgataat ttgacagtca atcctgtgga
    43681 taaaatctat atctgctttt tcagctgttt gttgaaataa aggatcaccc gtcttctcaa
    43741 ataacaacca attaattcct gtccaaaatc cttctgtcca accaatattg tctcttaatg
    43801 gataacgtcc ttttatagga cactcgcttg ggaattgata ctgactgtta ctgatacgtt
    43861 tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc attattccat tctttttcca
    43921 ttaattcttt ataacattca tttatccatt tttctggatg tattatctct tcaaacatca
    43981 ctttatcaag catttcccca ccagccttta gcataatttt agtctttttt tattgttttt
    44041 attttggata gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat tgttccatac
    44101 ttctaaacta taatcccgat cttgatgagc aaccactcct gttccgagcc aatgggttcc
    44161 ttttccaaat gtaccctcta atccaggaat aaaagtgttt tcccatgtca tcaagtttgt
    44221 attgggcaaa cccataatag ctgtggattt tcgtttaata gtatcgtcac ttaaacagat
    44281 aatacctgaa aagccagctt cactcaccct atagttttcc ttgtcttctt ctatgattat
    44341 tccttctttc tcaggagaac attcaatagc aaaccctgtc tcatatagaa catattctgt
    44401 atctaattct actttgtgta cgcgaatctg ccagccttca aaaggaataa gccaagttct
    44461 gatttttgcc tgtaaagtag gttcccatgt agaggcaagt acctctttct tacatacaat
    44521 gtctcgtaca ttatttcgaa cgatgaactg tccataggtt ttgatgctct cattcttaaa
    44581 actggtcgtg atatatgtgt ccgctcgctg aatccctagc attgaatcca ttgccatttc
    44641 tgctttcaat tgattcgctc gcggtatcga aaaaccaaat tgactagaat aagcaaattt
    44701 taaatatttt tcattggtca atgtatggaa atttggccct aattgtcccg cattcaaaaa
    44761 aaatgtatgt ccattatcat gttgtatgag caaattggct tctgaaagaa gtttagttcc
    44821 ttcacgaata ggcataggca tttctttagc cgtccaatac tcatggtctt caggtagcgc
    44881 caacaataaa aaaatcttat tcagccataa tggagataac gtactattat agggttcagt
    44941 caatgtcaat tgagggtagg tataaccaat attcaggata cctttttcat caaatatttc
    45001 tttttgcatc caccatctta tattccgatt gactaacccc ttgatttcac ctaatttaaa
    45061 aggcaaaaga tcggcccaca acatagctga ccaaaagctt gaaactgcaa aacgataaat
    45121 catgcttcga ccaaaaggca catttgcacc atcttccgca aaaaaatgaa tatagtctct
    45181 agaaaaatca atagctcggt tcttatatat ctggctaatg ggcttttctg gttctagtac
    45241 gctataaatc aaaccataat aatgaaatgc aaaaggatta taataatctt ggcgacctct
    45301 tgaactgtct cgataccagc cattttgaag ataacaattt tctatattat caaatgcttc
    45361 tttcagtgct gattctttaa ctggataacc aaatttttgg ataattgttc cgacaattat
    45421 cttaaaaaat tgccaattac catctgcaca ttcatattca aaaatttgcg agaaccaatg
    45481 aataatctgg cttttttctc ctgtatctaa ttttttccat aataattgtt catgatgata
    45541 taacgctaat aacattgcgg gcatttctac catacgctgg tctctttcat gtatttctcc
    45601 ccaataatcg ggatgggaag gatctgttcc agcttttatt cctgatagaa ttaatttcaa
    45661 ccatcgttca tctaattggt caattgcggg ccccgctccc cataaaaccc gagagaatcc
    45721 ttccattcct gcaattttat ttccatatcc taccccttga tgtatataat caatttgtgc
    45781 gccacttgct cgataaaacg gctggagtgg ttgatttatc tttttaaata agtcaaagta
    45841 atcctgtctg gtccttaata gtggtactgg atgtttcatt gtattttctc ctctttttac
    45901 taccaaatag ctaattccga ctgttctaat cgaagtaagc cttcaataaa aaaataatca
    45961 gaatagatca gtggcacatt ataagtcagt tctctatggt atgcttctgc tccgcctcca
    46021 atgatcccat cttcatccat attccaattt gcatagtgct tttctgtctt ttggagtata
    46081 ttgatagcag cttcatgata catttttgat cgtacagagg aaacatatcc tgctaattcc
    46141 agaagaccgc atgcagcgca tactccagca ctggtatcaa tattcgaatc ttttacagca
    46201 aaatcaatga gaggaatata accagttttt tcgattttct gaataaaatt tactgctatc
    46261 ttttctgcta tagctagata gtacggattt tttgtgtaac gataacttaa cgcgaatcca
    46321 tataaagccc atgcatttcc tctgctccaa caagaattct cattgtatcc ttgcccgcca
    46381 agtaaccgaa tatatttccc tgattctgca tcaaattctg cgatatgccc ggttgatcca
    46441 tctttcctca ctagatactt agctgttgta tctgcatgct gccgagcaat tttagagaac
    46501 ctcggatcac cagtttccct cgaagcccaa tacagtaatg gtaaattcat catactatca
    46561 ataatcatcc aaccaggttt atggtcattc catgctacta aatagttccc tgaaatatta
    46621 tagcgtccag caagtaccgt agctgcatgt aaccctagtt gtcgactatg tttgttttga
    46681 gactgttgat aatgtgcaat agccgatggt aagaataaaa aacctacatc atgatgaacg
    46741 tctgaaaaat ggtacagggc agattctaat aattgttctt gttgtattgc tgcctctttg
    46801 aacttttctc gttttgtctt gtgaaagagc tgccataaaa taccactcca aaaaccattg
    46861 gtccaccagt cgattcctct gtcctttatt acgtctgtat attttccgtt gtaagaaata
    46921 tatggaattt tattccccat tctgtctaat tccgcatcta tcttttcaga agctacattc
    46981 aaaatttttc ttgaccattc cttcgtctgt tcattcatgg atatcccgcc attcttttaa
    47041 tttttatgaa tgtggagttg ctcctagaac gaactagatg gttaacattc gttatttttt
    47101 ttacttattt gcagctccaa tgtcattctc cttaaattta gactgcgccc acacataaat
    47161 caagaccatt gggatcactg aagttgctaa aattagcata attgcgccat aattaattgg
    47221 ctcacctgta ctagaaaccg tcatattttt gataattaag ggaaccgtat atttatcttt
    47281 atctgaaagc atgactaatg gaaatagata ttgattccaa cttcccataa agataagtaa
    47341 accactagta ataatcgtag gtgataaaat cggtacagcg attttcgtaa aaatcaccca
    47401 ctcattggca ccatcgattc tggcagcctc catcagtgct gtaggaaatt gactgctata
    47461 ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa gggatgatca cacctgcata
    47521 agtattcgat aatcctaaac gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa
    47581 aggcaccatc ctagatagca taactgtatt gaacaacagt ttattcccca taaaacgata
    47641 ctttgctaat gcataacccg ccattgtaga actggctgta gctaaaactg tactgattac
    47701 agcaataaca atcgaattcc aaataatcaa gggataatta tatctcgcca tgatggcttg
    47761 ccagtttttt aatagcatat ccccaaactt gaaattgatt ccgccactta aaatttcttg
    47821 attactttgc gttgaagcta caatcatata taaatatggc atcaaaaata ttaaggcacc
    47881 aaacacaata atgacgtacg taaaaattct gctttgtatc ttttgtttag tcatttttat
    47941 cacctccgaa tttaaattga atagccgata atataacaga tacaaacacc atcgtccagg
    48001 caatagcaga ggcttttcca aaatcaacca tttcaaaact tgttttatat aaattgactc
    48061 ctacgaccaa agctgaattt tctggtccat tggaagtccc aaagaatata tttgggatat
    48121 tgaaattacc caagccattg atagtggcta aaactgtggc cattaaaatg attggcttta
    48181 gcatggggat cgttaataaa aagaattgcc gaaatttcga tgctccatct atttcagcac
    48241 tttcgtatat ctctttagaa atcccttgaa gaccagctaa aaataaaata gtgttatatc
    48301 ctacactaag ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa ggatttgtta
    48361 accactgtac accgttaatt cctagcgcat tcaaaaatcc attgaataac ccagtcggat
    48421 tgaaaaaagt catgaatacg gaagctacag ccaccgttga tgtaatggtt ggaatataat
    48481 agacagttct aaaaaatcct tttcctttta actgtgctga attcaataaa acagcaaagt
    48541 aaatagctaa tagcatcgtt attggaattg atcctgccat gataatcaaa acattcgtgt
    48601 aagatttaat aaacaatgga tctcggaaga tagctagata attgtcaaat ccaacaaatt
    48661 caaatgagta gccaattttc ataaaactaa aagcaaacga gataatgatg ggaatcaaaa
    48721 tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc ataagcactt tttttatgag
    48781 acgtatctgt tgcttttttc ttgctgttct ttattgtctt gcttggtaac gaagattgca
    48841 tcatcgttaa gtcctcctaa tattttttaa tcaccagtta attttacgcc gtacttatga
    48901 gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg ccaatagtta
    48961 tatgcagcgg tagtcaaata agacgtagca tctgaataag tggctggtaa aacagtattt
    49021 gatgtatttt cattgatttt attcaatagt tcatagtatt tttgtcctcc ttagtagtca
    49081 aactttttct cagctgcttc tgtttgatag gccaatgagt tagatgtagc cacgcccagc
    49141 tccagtgcgt tggctaagca ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc
    49201 acttgtggat tttttgcatc tttagggaca taaaaacttg atcctcctga tactgcacct
    49261 tttcctggat tttcttttga aaaaggaatc agtggagcca tgcgccattt gtcagatgca
    49321 tcaggaaaat tcaaagacat attcgtagca taccagccgc cagcaacaaa cattgcagat
    49381 tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc
    49441 atttctttga ttaactttgc agcatgtttc gcttctttag tattcagacg gatatttcct
    49501 tcttcatcaa gcaaagattg attctgctgg gctagcatgt tgataaacaa ttcttggtcc
    49561 ccagattctg gcaaggcaat tccaaaacta ccagtttttt cttttaatgc ttttgccacc
    49621 tggatatatt cttcccaaga actgatttct tcaaaatcta cacctatttc agcaaatttt
    49681 tttggttgat aataacaaag tgctggggta aaatcactag cccaaggaat aatcgtttca
    49741 ttactcgttt gttgacgcaa aatattcaat ttttgatcat aaaactgatc gccatatttg
    49801 tcataaaatc catattcatc agcactataa aaagcatctt gaaatttttc cagcacacct
    49861 gctgctgaag tgtctgccat ggagataata tccggcatat cttcgtgtgt taccaatttt
    49921 ggggtgatca tttgtactgt tgctgttccc gaagctacat tcacaatttt taagtcaata
    49981 tttgctccag ttttgcggtt atagattttt gcagcatttt caacatagtt tgtcagtttg
    50041 ccgtttgcga catatacttc taatgtagtg gcttttttta tcattggatc tgcgatgttt
    50101 gctgttactt cttcgttagt ttttccacat gcacttaagc tgaataatag ggtaatgttt
    50161 agcaaagcta tcaccaattt gtatcttttt ttcatgtcta tcccttcttt cttttcaagt
    50221 tcttatataa aacgctttca cttatgtata ttaacggaat tagta
//
LOCUS       DACCZY010000006        46542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCZY010000006 DACCZY010000000
VERSION     DACCZY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.006"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            28..909
                     /locus_tag="IVZ67_000246"
     CDS             28..909
                     /locus_tag="IVZ67_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164349.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="IVZ67_000247"
     CDS             922..2532
                     /locus_tag="IVZ67_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8164350.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="IVZ67_000248"
     CDS             complement(2609..3268)
                     /locus_tag="IVZ67_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8164351.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="IVZ67_000249"
     CDS             complement(3551..4216)
                     /locus_tag="IVZ67_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164352.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVZ67_000250"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVZ67_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8164353.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVZ67_000251"
     CDS             complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVZ67_000251"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8164354.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVZ67_000252"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVZ67_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8164355.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="IVZ67_000253"
     CDS             6530..6850
                     /locus_tag="IVZ67_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8164356.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7831)
                     /locus_tag="IVZ67_000254"
     CDS             complement(6962..7831)
                     /locus_tag="IVZ67_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8164357.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="IVZ67_000255"
     CDS             complement(7927..9231)
                     /locus_tag="IVZ67_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8164358.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="IVZ67_000256"
     CDS             complement(9228..9737)
                     /locus_tag="IVZ67_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164359.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="IVZ67_000257"
     CDS             complement(9912..12047)
                     /locus_tag="IVZ67_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8164360.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="IVZ67_000258"
     CDS             complement(12130..13785)
                     /locus_tag="IVZ67_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8164361.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="IVZ67_000259"
     CDS             complement(13787..14101)
                     /locus_tag="IVZ67_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8164362.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="IVZ67_000260"
     CDS             14271..14531
                     /locus_tag="IVZ67_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8164363.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="IVZ67_000261"
     CDS             14545..14859
                     /locus_tag="IVZ67_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8164364.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="IVZ67_000262"
     CDS             complement(15589..16143)
                     /locus_tag="IVZ67_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8164365.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="IVZ67_000263"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="IVZ67_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="IVZ67_000264"
     CDS             complement(18504..19220)
                     /locus_tag="IVZ67_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8164366.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVZ67_000265"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVZ67_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8164367.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVZ67_000266"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVZ67_000266"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8164368.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="IVZ67_000267"
     CDS             complement(22690..24150)
                     /locus_tag="IVZ67_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8164369.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="IVZ67_000268"
     CDS             complement(24140..25435)
                     /locus_tag="IVZ67_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8164370.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="IVZ67_000269"
     CDS             complement(25767..26444)
                     /locus_tag="IVZ67_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8164371.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="IVZ67_000270"
     CDS             complement(26474..27565)
                     /locus_tag="IVZ67_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8164372.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="IVZ67_000271"
     CDS             complement(27666..28100)
                     /locus_tag="IVZ67_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8164373.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVZ67_000272"
     CDS             complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVZ67_000272"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8164374.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="IVZ67_000273"
     CDS             complement(30138..32033)
                     /locus_tag="IVZ67_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164375.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="IVZ67_000274"
     CDS             complement(32091..33866)
                     /locus_tag="IVZ67_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8164376.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="IVZ67_000275"
     CDS             complement(33916..35412)
                     /locus_tag="IVZ67_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8164377.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="IVZ67_000276"
     CDS             complement(35606..37228)
                     /locus_tag="IVZ67_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8164378.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37556..38869)
                     /locus_tag="IVZ67_000277"
     CDS             complement(37556..38869)
                     /locus_tag="IVZ67_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8164379.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39114..39338)
                     /locus_tag="IVZ67_000278"
     CDS             complement(39114..39338)
                     /locus_tag="IVZ67_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164380.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39358..39726)
                     /locus_tag="IVZ67_000279"
     CDS             complement(39358..39726)
                     /locus_tag="IVZ67_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8164381.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="IVZ67_000280"
     CDS             complement(39922..40632)
                     /locus_tag="IVZ67_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164382.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="IVZ67_000281"
     CDS             complement(40650..41147)
                     /locus_tag="IVZ67_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164383.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="IVZ67_000282"
     CDS             complement(41184..41636)
                     /locus_tag="IVZ67_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8164384.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="IVZ67_000283"
     CDS             complement(41771..42376)
                     /locus_tag="IVZ67_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8164385.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="IVZ67_000284"
     CDS             complement(42826..43446)
                     /locus_tag="IVZ67_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8164386.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43448..44317)
                     /locus_tag="IVZ67_000285"
     CDS             complement(43448..44317)
                     /locus_tag="IVZ67_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8164387.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="IVZ67_000286"
     CDS             complement(44340..44747)
                     /locus_tag="IVZ67_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8164388.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="IVZ67_000287"
     CDS             complement(44766..46055)
                     /locus_tag="IVZ67_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8164389.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46113..>46542
                     /locus_tag="IVZ67_000288"
     CDS             46113..>46542
                     /locus_tag="IVZ67_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8164390.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13109 a         9900 c         7985 g        15548 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaga gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    37261 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    37321 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    37381 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    37441 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    37501 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    37561 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    37621 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    37681 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    37741 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    37801 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    37861 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    37921 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    37981 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    38041 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    38101 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    38161 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    38221 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    38281 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    38341 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    38401 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    38461 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    38521 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    38581 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    38641 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    38701 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    38761 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    38821 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    38881 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    38941 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    39001 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    39061 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    39121 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    39181 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    39241 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    39301 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    39361 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    39421 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    39481 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    39541 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    39601 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    39661 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    39721 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    39781 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    39841 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatgtttt gatgtgcacc ccaaaagtta
    46081 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
    46141 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
    46201 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
    46261 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
    46321 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
    46381 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
    46441 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
    46501 aagatgagtc tcttgaagaa gaagttattc gtttaagagc ag
//
LOCUS       DACCZY010000007        44859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCZY010000007 DACCZY010000000
VERSION     DACCZY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.007"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(2..1012)
                     /locus_tag="IVZ67_000289"
     CDS             complement(2..1012)
                     /locus_tag="IVZ67_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8164391.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="IVZ67_000290"
     CDS             complement(1192..1536)
                     /locus_tag="IVZ67_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8164392.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="IVZ67_000291"
     CDS             complement(1529..1963)
                     /locus_tag="IVZ67_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8164393.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="IVZ67_000292"
     CDS             2101..2838
                     /locus_tag="IVZ67_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164394.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="IVZ67_000293"
     CDS             2831..4042
                     /locus_tag="IVZ67_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164395.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4249..5028
                     /locus_tag="IVZ67_000294"
     CDS             4249..5028
                     /locus_tag="IVZ67_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8164396.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="IVZ67_000295"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="IVZ67_000295"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8164397.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVZ67_000296"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVZ67_000296"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8164398.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="cpaF"
                     /locus_tag="IVZ67_000297"
     CDS             7744..8673
                     /gene="cpaF"
                     /locus_tag="IVZ67_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8164399.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /locus_tag="IVZ67_000298"
     CDS             8630..9679
                     /locus_tag="IVZ67_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8164400.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /locus_tag="IVZ67_000299"
     CDS             9676..9954
                     /locus_tag="IVZ67_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8164401.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /locus_tag="IVZ67_000300"
     CDS             9929..10399
                     /locus_tag="IVZ67_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8164402.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10416..10718
                     /locus_tag="IVZ67_000301"
     CDS             10416..10718
                     /locus_tag="IVZ67_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164403.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10735..11106
                     /locus_tag="IVZ67_000302"
     CDS             10735..11106
                     /locus_tag="IVZ67_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8164404.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /locus_tag="IVZ67_000303"
     CDS             11078..11437
                     /locus_tag="IVZ67_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164405.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="IVZ67_000304"
     CDS             11609..12616
                     /locus_tag="IVZ67_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8164406.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="IVZ67_000305"
     CDS             12641..13825
                     /locus_tag="IVZ67_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8164407.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /locus_tag="IVZ67_000306"
     CDS             complement(14262..15845)
                     /locus_tag="IVZ67_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8164408.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16423..16566)
                     /locus_tag="IVZ67_000307"
     CDS             complement(16423..16566)
                     /locus_tag="IVZ67_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8164409.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="IVZ67_000308"
     CDS             complement(16789..17268)
                     /locus_tag="IVZ67_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8164410.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="IVZ67_000309"
     CDS             complement(17341..17625)
                     /locus_tag="IVZ67_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164411.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="IVZ67_000310"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVZ67_000310"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="IVZ67_000311"
     CDS             complement(17969..19255)
                     /locus_tag="IVZ67_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8164412.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="IVZ67_000312"
     CDS             19393..20064
                     /locus_tag="IVZ67_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8164413.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="IVZ67_000313"
     CDS             20030..20518
                     /locus_tag="IVZ67_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8164414.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="IVZ67_000314"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="IVZ67_000314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8164415.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="IVZ67_000315"
     CDS             21479..22243
                     /locus_tag="IVZ67_000315"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8164416.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="IVZ67_000316"
     CDS             22476..24689
                     /locus_tag="IVZ67_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8164417.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /locus_tag="IVZ67_000317"
     CDS             24700..25146
                     /locus_tag="IVZ67_000317"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8164418.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="IVZ67_000318"
     CDS             25317..26576
                     /locus_tag="IVZ67_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8164419.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="IVZ67_000319"
     CDS             complement(26665..27612)
                     /locus_tag="IVZ67_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8164420.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /locus_tag="IVZ67_000320"
     CDS             28006..29298
                     /locus_tag="IVZ67_000320"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8164421.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="IVZ67_000321"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="IVZ67_000321"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8164422.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="IVZ67_000322"
     CDS             31191..32066
                     /locus_tag="IVZ67_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8164423.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="IVZ67_000323"
     CDS             32332..34215
                     /locus_tag="IVZ67_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8164424.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="IVZ67_000324"
     CDS             complement(34290..34574)
                     /locus_tag="IVZ67_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8164425.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="IVZ67_000325"
     CDS             complement(34564..35298)
                     /locus_tag="IVZ67_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8164426.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="IVZ67_000326"
     CDS             35427..36050
                     /locus_tag="IVZ67_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8164427.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="IVZ67_000327"
     CDS             36242..37363
                     /locus_tag="IVZ67_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8164428.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="IVZ67_000328"
     CDS             37370..40498
                     /locus_tag="IVZ67_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8164429.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="IVZ67_000329"
     CDS             40517..43711
                     /locus_tag="IVZ67_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8164430.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="IVZ67_000330"
     CDS             complement(43857..44564)
                     /locus_tag="IVZ67_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8164431.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14394 a         7966 c         9407 g        13092 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt ttttaataca gtctcaatat aactattca
//
LOCUS       DACCZY010000008        44718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCZY010000008 DACCZY010000000
VERSION     DACCZY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.008"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            81..473
                     /locus_tag="IVZ67_000331"
     CDS             81..473
                     /locus_tag="IVZ67_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8164432.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            501..1841
                     /gene="glnA"
                     /locus_tag="IVZ67_000332"
     CDS             501..1841
                     /gene="glnA"
                     /locus_tag="IVZ67_000332"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8164433.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3034..4644
                     /locus_tag="IVZ67_000333"
     CDS             3034..4644
                     /locus_tag="IVZ67_000333"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8164434.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            4936..5805
                     /locus_tag="IVZ67_000334"
     CDS             4936..5805
                     /locus_tag="IVZ67_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8164435.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5970..7241
                     /locus_tag="IVZ67_000335"
     CDS             5970..7241
                     /locus_tag="IVZ67_000335"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8164436.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7238..8533
                     /locus_tag="IVZ67_000336"
     CDS             7238..8533
                     /locus_tag="IVZ67_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8164437.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8632..8895
                     /locus_tag="IVZ67_000337"
     CDS             8632..8895
                     /locus_tag="IVZ67_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8164438.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9340..9789
                     /locus_tag="IVZ67_000338"
     CDS             9340..9789
                     /locus_tag="IVZ67_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8164439.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9846..11264
                     /locus_tag="IVZ67_000339"
     CDS             9846..11264
                     /locus_tag="IVZ67_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164440.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11264..12124
                     /locus_tag="IVZ67_000340"
     CDS             11264..12124
                     /locus_tag="IVZ67_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8164441.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12298..13071
                     /locus_tag="IVZ67_000341"
     CDS             12298..13071
                     /locus_tag="IVZ67_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8164442.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13218..13583
                     /locus_tag="IVZ67_000342"
     CDS             13218..13583
                     /locus_tag="IVZ67_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8164443.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13731..14939)
                     /locus_tag="IVZ67_000343"
     CDS             complement(13731..14939)
                     /locus_tag="IVZ67_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8164444.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14958..15236)
                     /locus_tag="IVZ67_000344"
     CDS             complement(14958..15236)
                     /locus_tag="IVZ67_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164445.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15429..16085
                     /locus_tag="IVZ67_000345"
     CDS             15429..16085
                     /locus_tag="IVZ67_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8164446.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16527..17324)
                     /locus_tag="IVZ67_000346"
     CDS             complement(16527..17324)
                     /locus_tag="IVZ67_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8164447.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17321..18505)
                     /locus_tag="IVZ67_000347"
     CDS             complement(17321..18505)
                     /locus_tag="IVZ67_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8164448.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18530..19777)
                     /locus_tag="IVZ67_000348"
     CDS             complement(18530..19777)
                     /locus_tag="IVZ67_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8164449.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            19992..21737
                     /gene="dnaX"
                     /locus_tag="IVZ67_000349"
     CDS             19992..21737
                     /gene="dnaX"
                     /locus_tag="IVZ67_000349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8164450.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21757..22071
                     /locus_tag="IVZ67_000350"
     CDS             21757..22071
                     /locus_tag="IVZ67_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8164451.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22096..22692
                     /gene="recR"
                     /locus_tag="IVZ67_000351"
     CDS             22096..22692
                     /gene="recR"
                     /locus_tag="IVZ67_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8164452.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22756..23400
                     /locus_tag="IVZ67_000352"
     CDS             22756..23400
                     /locus_tag="IVZ67_000352"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8164453.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23415..23744
                     /locus_tag="IVZ67_000353"
     CDS             23415..23744
                     /locus_tag="IVZ67_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164454.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23758..24696
                     /gene="holB"
                     /locus_tag="IVZ67_000354"
     CDS             23758..24696
                     /gene="holB"
                     /locus_tag="IVZ67_000354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8164455.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24732..25556
                     /locus_tag="IVZ67_000355"
     CDS             24732..25556
                     /locus_tag="IVZ67_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8164456.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25549..25896
                     /locus_tag="IVZ67_000356"
     CDS             25549..25896
                     /locus_tag="IVZ67_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8164457.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25965..26837
                     /gene="rsmI"
                     /locus_tag="IVZ67_000357"
     CDS             25965..26837
                     /gene="rsmI"
                     /locus_tag="IVZ67_000357"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8164458.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26946..28067
                     /gene="dinB"
                     /locus_tag="IVZ67_000358"
     CDS             26946..28067
                     /gene="dinB"
                     /locus_tag="IVZ67_000358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8164459.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28121..28723)
                     /gene="nrdG"
                     /locus_tag="IVZ67_000359"
     CDS             complement(28121..28723)
                     /gene="nrdG"
                     /locus_tag="IVZ67_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8164460.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29038..31227)
                     /gene="nrdD"
                     /locus_tag="IVZ67_000360"
     CDS             complement(29038..31227)
                     /gene="nrdD"
                     /locus_tag="IVZ67_000360"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8164461.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31553..32320
                     /locus_tag="IVZ67_000361"
     CDS             31553..32320
                     /locus_tag="IVZ67_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164462.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32310..34352
                     /locus_tag="IVZ67_000362"
     CDS             32310..34352
                     /locus_tag="IVZ67_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164463.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34633..34782
                     /locus_tag="IVZ67_000363"
     CDS             34633..34782
                     /locus_tag="IVZ67_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8164464.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34793..36307
                     /gene="dltA"
                     /locus_tag="IVZ67_000364"
     CDS             34793..36307
                     /gene="dltA"
                     /locus_tag="IVZ67_000364"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8164465.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36304..37509
                     /gene="dltB"
                     /locus_tag="IVZ67_000365"
     CDS             36304..37509
                     /gene="dltB"
                     /locus_tag="IVZ67_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8164466.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37554..37787
                     /gene="dltC"
                     /locus_tag="IVZ67_000366"
     CDS             37554..37787
                     /gene="dltC"
                     /locus_tag="IVZ67_000366"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8164467.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37790..39058
                     /gene="dltD"
                     /locus_tag="IVZ67_000367"
     CDS             37790..39058
                     /gene="dltD"
                     /locus_tag="IVZ67_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8164468.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39199..40281
                     /locus_tag="IVZ67_000368"
     CDS             39199..40281
                     /locus_tag="IVZ67_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8164469.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40281..41084
                     /locus_tag="IVZ67_000369"
     CDS             40281..41084
                     /locus_tag="IVZ67_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8164470.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41154..41894
                     /locus_tag="IVZ67_000370"
     CDS             41154..41894
                     /locus_tag="IVZ67_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8164471.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41905..42909
                     /locus_tag="IVZ67_000371"
     CDS             41905..42909
                     /locus_tag="IVZ67_000371"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8164472.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42954..44717)
                     /locus_tag="IVZ67_000372"
     CDS             complement(42954..44717)
                     /locus_tag="IVZ67_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164473.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14360 a         7815 c         9323 g        13220 t
ORIGIN      
        1 aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta gccaacttat
       61 aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg tccgtatttc
      121 caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc tattatgaag
      181 aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat tcgctgaatg
      241 atattgatat gttgcttgag attaaagact atctttcaga gggattgaat atggctggga
      301 tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac aagaagccgt
      361 tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa ggtgggttaa
      421 gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac tgaataaatt
      481 aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa atcaaacgaa
      541 tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc ttaggtacga
      601 tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat aaaatgatgt
      661 ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg tatttgtatc
      721 cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa gttgctcgtt
      781 taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct cgcgggaatt
      841 tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat ttaggacctg
      901 aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg accttgaatg
      961 ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc cgccgtgaca
     1021 tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat gaagtagcgc
     1081 caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt gataatatcc
     1141 aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac gcgactttca
     1201 tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg tctttgttca
     1261 atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt gaaacagctt
     1321 atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt tgcaatccaa
     1381 cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat gtcgcatgga
     1441 gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta tccacacgtt
     1501 tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca gtattgttgc
     1561 aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt gaccgtaaca
     1621 tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta ccttcaacta
     1681 tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca ttaggacaac
     1741 atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc agacaaactg
     1801 tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat accgtgaatt
     1861 agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga aaaatagttc
     1921 ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttagt
     1981 ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag caaaaacagc
     2041 ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt gaacaccgtt
     2101 taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     2161 ggctttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
     2221 tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata atttcaaaaa
     2281 cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca ggagaacacc
     2341 gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt tacttatgtg
     2401 attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg tcatctttga
     2461 aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata tgccaaaaat
     2521 ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga aagaaaaaga
     2581 ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta gttctttgtt
     2641 aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat ttcacttaaa
     2701 atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt ttatatcaag
     2761 cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat aatttctcaa
     2821 aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac ttgactttcc
     2881 cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa gtttacagaa
     2941 atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct gagacttttc
     3001 ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt tgtgaccggc
     3061 ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg cttattgaaa
     3121 aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt ggatccgggg
     3181 acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc tgaaacagat
     3241 ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata ctccaacgtg
     3301 acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga atacttggga
     3361 gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat catgcgagca
     3421 gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt aggggatata
     3481 gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg tacagataat
     3541 gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga aatgaaaact
     3601 aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc gaatattttg
     3661 gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc acaattttgt
     3721 gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta ctccatccca
     3781 ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa actagataca
     3841 ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa cttgaagaaa
     3901 aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata tatctcagta
     3961 gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat cgattggatc
     4021 aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc agatggaatc
     4081 ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc gattcgttat
     4141 gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat ggcttgtgtc
     4201 gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac gaatccagat
     4261 acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa tttgggtgga
     4321 actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac ggctgaagca
     4381 tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt caataacaaa
     4441 tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc agataaccgt
     4501 ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca attccaccca
     4561 gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt cggagcatca
     4621 ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa gtagcaagag
     4681 aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat tatttgtagg
     4741 aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata ttttattttt
     4801 tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac taccgaaatg
     4861 tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt tacaaaactt
     4921 aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc tgctcgtaaa
     4981 ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca agcaatcctt
     5041 gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg tgcagctaaa
     5101 tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga agcaatgaac
     5161 atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc tttggaatgt
     5221 atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt tgaagaaaac
     5281 ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc agttgaatgt
     5341 gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga attagctgat
     5401 attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg cggtatcggt
     5461 aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca cttacaagca
     5521 attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc aggtattcct
     5581 caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt taacactgaa
     5641 ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa agataaagaa
     5701 ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat cgttgaagat
     5761 gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc cacataacag
     5821 atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt ttttacttaa
     5881 aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc atatccatga
     5941 aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa ggaaaccgac
     6001 ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca ttgattccag
     6061 ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata caagatgttc
     6121 attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc aacaacatac
     6181 tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa atcaatagtt
     6241 tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa gcggtagtag
     6301 gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc aaaggatttg
     6361 aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg acagaaaata
     6421 aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca acgatcaatg
     6481 tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct gcacgagaac
     6541 cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt cgaggagcgg
     6601 gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg catttcatga
     6661 ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta ggaaatggta
     6721 tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa ttgcaggaaa
     6781 ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg aaagaattaa
     6841 aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg cagcagccac
     6901 ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg atctatgcga
     6961 aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc gtggaaggaa
     7021 atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg gcttctgatc
     7081 ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca acgacgattt
     7141 acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta acagccttag
     7201 gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt tgacaatggc
     7261 agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat ttaaaattcc
     7321 ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg cacaagcaga
     7381 gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag atggttacgg
     7441 ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt ctgcctcaca
     7501 aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc gtccacctaa
     7561 agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa aagatccgga
     7621 agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc aaagacagct
     7681 tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct tttctccaat
     7741 cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa cttctgtatt
     7801 aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa tcgttctact
     7861 gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag gcgacgtggt
     7921 atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat tagtattgga
     7981 acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg atagtattac
     8041 tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat tgagtggcgg
     8101 gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa gaaacatcga
     8161 agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa gccgtatgga
     8221 tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac tttcacgtga
     8281 attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta cccgaaaaga
     8341 agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa aaaatatgac
     8401 tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa caaaaaacaa
     8461 tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc cgaccagaaa
     8521 tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt tgtaaatcga
     8581 caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat catgaaagaa
     8641 aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg tttcaaattc
     8701 ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg taatacatac
     8761 ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg acgtcaaaaa
     8821 ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct caaagacgca
     8881 aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat ctgataggga
     8941 tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg aaaaagtcgt
     9001 aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat ccacttgata
     9061 aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa ccatttacct
     9121 aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat agacttgcga
     9181 ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat gggtcttttt
     9241 cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga cctcagttcc
     9301 aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga tcaattgatc
     9361 gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt atttgagcga
     9421 ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt cgttaaggca
     9481 ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg gattccccat
     9541 gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc caaattaacg
     9601 gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt agttcctgaa
     9661 aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt gatgaatgca
     9721 gattttagaa agggaataaa agaagccact acgccagcag atatcatcag acagattagt
     9781 caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa aggaggaaaa
     9841 taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat acttatatgt
     9901 cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa ttcgaaacac
     9961 aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca gatgcaatca
    10021 ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa gtaaagcgtg
    10081 tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca ttgagagata
    10141 ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa aagccgacaa
    10201 tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc gcaggcggaa
    10261 tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac tcagcagact
    10321 ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt gccatgatgg
    10381 ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt tttgccccag
    10441 gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc ggaatgattg
    10501 gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc gtaaaaaaag
    10561 aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta attattgggc
    10621 ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct ttgacaaatt
    10681 ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc gggatcatga
    10741 tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg atcggtttga
    10801 tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg acacctcccc
    10861 taggtattgc tttagccact ttattattca aacataaatt taccaaagaa gaacggactt
    10921 caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct ataccgtttg
    10981 cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca gtcgctgcag
    11041 ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt tgggtgatgc
    11101 taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca tcaggtacag
    11161 tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa aaacaagaca
    11221 agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat atacaatgaa
    11281 agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca atatttgcag
    11341 ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac cagtaatctt
    11401 agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg cagtaagagc
    11461 ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg gtacgataga
    11521 agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg cgtctttgaa
    11581 atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc atgcggtaaa
    11641 tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg aaggaggtgc
    11701 tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag aaacaggaat
    11761 cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa acagaactcc
    11821 aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc catttgtttt
    11881 acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat acggagttgc
    11941 caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga gagaaatatt
    12001 gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta ataaaaaggt
    12061 acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg ctagtttata
    12121 ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata ccctcttaac
    12181 cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct cgaagccaat
    12241 cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg tgattttatg
    12301 ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa caaccaattt
    12361 gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat taaacgtgac
    12421 cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc catgcttatt
    12481 gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga agaagaaaca
    12541 gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat ggtcttattc
    12601 ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt tgatggtttg
    12661 cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac aacggcaaaa
    12721 gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg ttcaaaagcc
    12781 tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg cgggctagat
    12841 ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa agcttttaga
    12901 aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca taaattcatg
    12961 catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga actgacaaaa
    13021 gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata aattattacg
    13081 tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct cgcttctctc
    13141 agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt tttatccagg
    13201 agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca atcatgatct
    13261 gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg gattcaacga
    13321 ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg atcttacgta
    13381 aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt atcttgtggc
    13441 tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg attcctgctc
    13501 tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa ggcatcgtac
    13561 cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat aaatgaaagc
    13621 tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac tcaaaaaacc
    13681 gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga tcaaatttca
    13741 tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg gccattaaca
    13801 agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa tcctctaaat
    13861 gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa
    13921 ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat ttttaaacaa
    13981 acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc tccagagata
    14041 agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt taagaataaa
    14101 tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc agtcaatgga
    14161 cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc agcaactaag
    14221 tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga aagacccatc
    14281 aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt gatattccat
    14341 gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg aaagacaagg
    14401 caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa acgaaaatca
    14461 gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa actagtactt
    14521 aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc agtaacgata
    14581 gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg aactaaatac
    14641 aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata gaagatcgcg
    14701 ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat gaataagcgt
    14761 gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct caagctggcg
    14821 tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata aatcaggtag
    14881 acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt cttttccatg
    14941 tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg aacttctaca
    15001 tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc tcttgtagac
    15061 ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac tgctttgatc
    15121 aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt catatcaaat
    15181 cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta attcatgttt
    15241 tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa aaaggaaaaa
    15301 aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag taaaatacct
    15361 ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg
    15421 gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta
    15481 tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata
    15541 tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc
    15601 ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca
    15661 tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc
    15721 gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt
    15781 attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa
    15841 agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag
    15901 aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca
    15961 tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca
    16021 ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac aatattatct
    16081 tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
    16141 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    16201 acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt
    16261 caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga
    16321 gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc
    16381 aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct
    16441 tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt
    16501 ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg
    16561 ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg
    16621 attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata
    16681 ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca
    16741 gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct
    16801 actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt
    16861 cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca
    16921 ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg
    16981 ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc
    17041 atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta
    17101 tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct
    17161 ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg
    17221 aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca
    17281 acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag taacctccga
    17341 tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct
    17401 ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga
    17461 cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat
    17521 tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat
    17581 ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt
    17641 tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt
    17701 taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg
    17761 tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa
    17821 cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg
    17881 taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca
    17941 cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt
    18001 tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta
    18061 ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt
    18121 agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac
    18181 aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac
    18241 cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg
    18301 ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac
    18361 gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc
    18421 agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag
    18481 gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg
    18541 ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat
    18601 ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag
    18661 gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac
    18721 atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt
    18781 tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc
    18841 ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca
    18901 taccacgtgc aatggaacct tgcacgtagc cttcacgtac gctaggatca cctagcatcg
    18961 ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta
    19021 cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat
    19081 ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg
    19141 cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat
    19201 tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa
    19261 tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat
    19321 agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt
    19381 ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt
    19441 caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat
    19501 ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca
    19561 aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg
    19621 ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga
    19681 ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc
    19741 cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag
    19801 ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga
    19861 ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa
    19921 ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa
    19981 gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag
    20041 acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa
    20101 cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga
    20161 ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg
    20221 aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca
    20281 gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa
    20341 aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca
    20401 atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga
    20461 ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac
    20521 ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca
    20581 gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg
    20641 cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg
    20701 ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg
    20761 tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag
    20821 aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc
    20881 aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg
    20941 cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa
    21001 atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg
    21061 cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag
    21121 taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta
    21181 atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga
    21241 caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac
    21301 agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc
    21361 agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg
    21421 cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg
    21481 tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa
    21541 gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag
    21601 aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag
    21661 aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta
    21721 cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg
    21781 caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg
    21841 ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca
    21901 ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc
    21961 gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg
    22021 gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact
    22081 gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg
    22141 aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg
    22201 aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat
    22261 ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct
    22321 tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa
    22381 aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa
    22441 gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat
    22501 gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat
    22561 ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct
    22621 gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga
    22681 cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga
    22741 gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga
    22801 aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc
    22861 attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt
    22921 gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga
    22981 agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc
    23041 gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc
    23101 gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat
    23161 cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc
    23221 gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag
    23281 ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa
    23341 gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag
    23401 gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt
    23461 tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct
    23521 tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag
    23581 cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta
    23641 cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg
    23701 ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg
    23761 aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt
    23821 tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa
    23881 catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg
    23941 ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg
    24001 gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa
    24061 ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa
    24121 atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt
    24181 ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt
    24241 caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag
    24301 cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca
    24361 gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc
    24421 gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa
    24481 tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag
    24541 gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag
    24601 ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct
    24661 gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa
    24721 ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt
    24781 attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc
    24841 aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc
    24901 ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa
    24961 aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg
    25021 agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt
    25081 ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat
    25141 ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg
    25201 gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg
    25261 tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt
    25321 tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa
    25381 aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag
    25441 ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg
    25501 aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga
    25561 tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt
    25621 gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa
    25681 aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa
    25741 aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga
    25801 ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt
    25861 tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc
    25921 ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa
    25981 gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt
    26041 tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg
    26101 aggatacgcg taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa
    26161 gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag
    26221 gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg
    26281 agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag
    26341 ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct
    26401 tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag
    26461 ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg aatttcttag
    26521 aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat
    26581 accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg
    26641 aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag
    26701 agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg
    26761 ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt
    26821 accatgaatt agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca
    26881 gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg
    26941 tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata
    27001 ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac
    27061 atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag
    27121 caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg
    27181 agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca
    27241 aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg
    27301 aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc
    27361 gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt
    27421 acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag
    27481 ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg
    27541 tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat
    27601 atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc
    27661 gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg
    27721 gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc
    27781 gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag
    27841 tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag
    27901 aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct
    27961 tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga
    28021 cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag
    28081 agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt
    28141 tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg
    28201 attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt
    28261 ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga
    28321 gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc
    28381 agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat
    28441 ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca
    28501 agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat cgaaaatttt
    28561 gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt
    28621 tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc
    28681 tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat
    28741 ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca
    28801 aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa
    28861 gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa
    28921 taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag
    28981 gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca
    29041 tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc
    29101 ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg
    29161 atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc
    29221 acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc
    29281 aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta
    29341 gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa
    29401 tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt
    29461 gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact
    29521 ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc
    29581 attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc
    29641 ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac
    29701 agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc
    29761 gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc
    29821 tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa
    29881 tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc
    29941 taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa
    30001 tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt
    30061 cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta
    30121 attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa
    30181 gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg
    30241 aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg
    30301 tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat
    30361 atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc
    30421 ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc
    30481 tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga
    30541 gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt
    30601 gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata
    30661 aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg
    30721 tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg
    30781 tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt
    30841 gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt
    30901 ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg
    30961 ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc
    31021 ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc
    31081 gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac
    31141 atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac
    31201 agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta
    31261 tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg
    31321 ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa
    31381 acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat
    31441 aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga
    31501 tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa
    31561 gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc
    31621 cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag
    31681 tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg
    31741 cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt
    31801 tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc
    31861 aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa
    31921 gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga
    31981 tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc
    32041 acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt
    32101 gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca
    32161 tgatccatat acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt
    32221 ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca
    32281 ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt
    32341 ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct
    32401 attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata
    32461 cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt
    32521 tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc
    32581 agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa
    32641 ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt
    32701 caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc
    32761 catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt
    32821 caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag
    32881 atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac
    32941 ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca
    33001 gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg
    33061 tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta
    33121 aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt
    33181 tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga
    33241 caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc
    33301 cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg
    33361 aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata
    33421 gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc
    33481 aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata
    33541 ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta
    33601 ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg
    33661 acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag
    33721 caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa
    33781 tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt
    33841 atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc
    33901 aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt
    33961 cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg
    34021 ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag
    34081 aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca
    34141 gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca
    34201 ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt
    34261 tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga
    34321 gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata
    34381 gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca
    34441 acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat
    34501 ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg
    34561 taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga
    34621 ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc
    34681 atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat
    34741 aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat
    34801 caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta
    34861 tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta
    34921 ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt
    34981 tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga
    35041 agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt
    35101 catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga
    35161 atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac
    35221 agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat
    35281 cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc
    35341 tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta
    35401 tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt
    35461 tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc
    35521 atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga
    35581 catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg
    35641 attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc
    35701 aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt
    35761 aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac
    35821 gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa
    35881 gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg
    35941 tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt
    36001 acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat
    36061 caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt
    36121 tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga
    36181 acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct
    36241 gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc
    36301 tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc
    36361 attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat
    36421 caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag
    36481 ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt
    36541 tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt
    36601 ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt
    36661 tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat
    36721 ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca
    36781 atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct
    36841 gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt
    36901 ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc
    36961 attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt
    37021 atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg
    37081 atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa
    37141 ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga
    37201 ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc
    37261 ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc
    37321 gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag
    37381 aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg
    37441 acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt
    37501 tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag
    37561 aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg
    37621 atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg
    37681 agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga gaggattggt
    37741 gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa
    37801 actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac
    37861 ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat
    37921 gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata
    37981 tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct
    38041 ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc
    38101 gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt
    38161 ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac
    38221 gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc
    38281 tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt
    38341 gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca
    38401 gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt
    38461 gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga
    38521 aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt
    38581 cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca
    38641 gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt
    38701 ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa
    38761 atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa
    38821 atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac
    38881 caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca
    38941 acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga
    39001 tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag
    39061 aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata
    39121 aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat
    39181 agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct
    39241 gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa
    39301 ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct
    39361 ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca
    39421 aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc
    39481 atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt
    39541 ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag
    39601 gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt
    39661 cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta
    39721 ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat
    39781 ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta
    39841 ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta
    39901 gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta
    39961 ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg
    40021 aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct
    40081 ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc
    40141 attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat
    40201 ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa
    40261 aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag
    40321 cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa
    40381 tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca
    40441 tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg
    40501 cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat
    40561 tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag
    40621 tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga
    40681 gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag
    40741 attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca
    40801 tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg
    40861 atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta
    40921 cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt
    40981 tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac
    41041 aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat
    41101 tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat
    41161 taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt
    41221 tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat
    41281 tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg
    41341 atcatattgc tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc
    41401 cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga
    41461 cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc
    41521 cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg
    41581 tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt
    41641 tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa
    41701 atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta
    41761 acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa
    41821 atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag
    41881 tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga
    41941 atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg
    42001 ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa
    42061 atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc
    42121 ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg
    42181 gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg
    42241 ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt
    42301 tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc
    42361 gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca
    42421 aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa
    42481 aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta
    42541 gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat
    42601 cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc
    42661 aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca
    42721 gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct
    42781 gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc
    42841 agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta
    42901 ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca
    42961 ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc
    43021 tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt
    43081 tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca
    43141 ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt
    43201 cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc
    43261 aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag
    43321 gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc
    43381 acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt
    43441 tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc
    43501 tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca
    43561 taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg
    43621 tcggtattta agatgtgatc gattagattg tagctgataa taacgattag aatgataata
    43681 tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa
    43741 atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg
    43801 ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta
    43861 gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc
    43921 agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag
    43981 taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa
    44041 tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat
    44101 acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga
    44161 cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca
    44221 gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt
    44281 ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag
    44341 actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag
    44401 agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga
    44461 tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt
    44521 tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg
    44581 tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt
    44641 atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt
    44701 cctgttaaaa ataacaat
//
LOCUS       DACCZY010000009        36833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCZY010000009 DACCZY010000000
VERSION     DACCZY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.009"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            309..1418
                     /gene="pdhA"
                     /locus_tag="IVZ67_000373"
     CDS             309..1418
                     /gene="pdhA"
                     /locus_tag="IVZ67_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8164474.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1421..2398
                     /locus_tag="IVZ67_000374"
     CDS             1421..2398
                     /locus_tag="IVZ67_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8164475.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2423..4066
                     /locus_tag="IVZ67_000375"
     CDS             2423..4066
                     /locus_tag="IVZ67_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8164476.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4074..5480
                     /gene="lpdA"
                     /locus_tag="IVZ67_000376"
     CDS             4074..5480
                     /gene="lpdA"
                     /locus_tag="IVZ67_000376"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8164477.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5642..5917
                     /locus_tag="IVZ67_000377"
     CDS             5642..5917
                     /locus_tag="IVZ67_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8164478.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5923..6690
                     /locus_tag="IVZ67_000378"
     CDS             5923..6690
                     /locus_tag="IVZ67_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8164479.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6858..8687
                     /gene="typA"
                     /locus_tag="IVZ67_000379"
     CDS             6858..8687
                     /gene="typA"
                     /locus_tag="IVZ67_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8164480.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8948..10720
                     /gene="recQ"
                     /locus_tag="IVZ67_000380"
     CDS             8948..10720
                     /gene="recQ"
                     /locus_tag="IVZ67_000380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8164481.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10834..11136
                     /locus_tag="IVZ67_000381"
     CDS             10834..11136
                     /locus_tag="IVZ67_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8164482.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11139..12302
                     /locus_tag="IVZ67_000382"
     CDS             11139..12302
                     /locus_tag="IVZ67_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8164483.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12328..15756
                     /locus_tag="IVZ67_000383"
     CDS             12328..15756
                     /locus_tag="IVZ67_000383"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8164484.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15777..16901
                     /locus_tag="IVZ67_000384"
     CDS             15777..16901
                     /locus_tag="IVZ67_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164485.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16891..17328
                     /locus_tag="IVZ67_000385"
     CDS             16891..17328
                     /locus_tag="IVZ67_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8164486.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17312..17650
                     /locus_tag="IVZ67_000386"
     CDS             17312..17650
                     /locus_tag="IVZ67_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8164487.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17769..18329
                     /gene="rsmD"
                     /locus_tag="IVZ67_000387"
     CDS             17769..18329
                     /gene="rsmD"
                     /locus_tag="IVZ67_000387"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8164488.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18326..18817
                     /gene="coaD"
                     /locus_tag="IVZ67_000388"
     CDS             18326..18817
                     /gene="coaD"
                     /locus_tag="IVZ67_000388"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8164489.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18807..19850
                     /locus_tag="IVZ67_000389"
     CDS             18807..19850
                     /locus_tag="IVZ67_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8164490.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20009..20737
                     /locus_tag="IVZ67_000390"
     CDS             20009..20737
                     /locus_tag="IVZ67_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8164491.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20853..21098
                     /locus_tag="IVZ67_000391"
     CDS             20853..21098
                     /locus_tag="IVZ67_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8164492.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21095..21313
                     /locus_tag="IVZ67_000392"
     CDS             21095..21313
                     /locus_tag="IVZ67_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8164493.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21449..21988
                     /locus_tag="IVZ67_000393"
     CDS             21449..21988
                     /locus_tag="IVZ67_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8164494.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            21981..24254
                     /locus_tag="IVZ67_000394"
     CDS             21981..24254
                     /locus_tag="IVZ67_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8164495.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24292..25338
                     /gene="holA"
                     /locus_tag="IVZ67_000395"
     CDS             24292..25338
                     /gene="holA"
                     /locus_tag="IVZ67_000395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8164496.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25427..25681)
                     /locus_tag="IVZ67_000396"
     CDS             complement(25427..25681)
                     /locus_tag="IVZ67_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8164497.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26081..26350)
                     /locus_tag="IVZ67_000397"
     CDS             complement(26081..26350)
                     /locus_tag="IVZ67_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164498.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26820..27275)
                     /locus_tag="IVZ67_000398"
     CDS             complement(26820..27275)
                     /locus_tag="IVZ67_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8164499.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27413..29341)
                     /locus_tag="IVZ67_000399"
     CDS             complement(27413..29341)
                     /locus_tag="IVZ67_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8164500.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29538..30371)
                     /locus_tag="IVZ67_000400"
     CDS             complement(29538..30371)
                     /locus_tag="IVZ67_000400"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8164501.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30563..31267)
                     /locus_tag="IVZ67_000401"
     CDS             complement(30563..31267)
                     /locus_tag="IVZ67_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8164502.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31290..31916)
                     /locus_tag="IVZ67_000402"
     CDS             complement(31290..31916)
                     /locus_tag="IVZ67_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8164503.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32242..34617
                     /locus_tag="IVZ67_000403"
     CDS             32242..34617
                     /locus_tag="IVZ67_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8164504.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34641..35132)
                     /locus_tag="IVZ67_000404"
     CDS             complement(34641..35132)
                     /locus_tag="IVZ67_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8164505.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35319..36239
                     /locus_tag="IVZ67_000405"
     CDS             35319..36239
                     /locus_tag="IVZ67_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8164506.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36393..36737
                     /locus_tag="IVZ67_000406"
     CDS             36393..36737
                     /locus_tag="IVZ67_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8164507.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        12221 a         6475 c         7673 g        10464 t
ORIGIN      
        1 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
       61 agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac
      121 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
      181 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
      241 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
      301 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
      361 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
      421 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
      481 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
      541 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
      601 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
      661 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
      721 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
      781 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
      841 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
      901 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
      961 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
     1021 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
     1081 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
     1141 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
     1201 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
     1261 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
     1321 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
     1381 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
     1441 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
     1501 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
     1561 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
     1621 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
     1681 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
     1741 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
     1801 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
     1861 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
     1921 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
     1981 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
     2041 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
     2101 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
     2161 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
     2221 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
     2281 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
     2341 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
     2401 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg
     2461 aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga
     2521 tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac
     2581 aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt
     2641 agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga
     2701 atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc
     2761 gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg
     2821 tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact
     2881 agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa
     2941 aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga
     3001 tgctcctgga cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga
     3061 aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt
     3121 attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt
     3181 aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg
     3241 tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc
     3301 agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg
     3361 agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt
     3421 caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact
     3481 atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt
     3541 cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc
     3601 atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc
     3661 aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat
     3721 gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc
     3781 tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg
     3841 ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc
     3901 acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc
     3961 attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat
     4021 caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag
     4081 ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg
     4141 tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata
     4201 ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac
     4261 accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac
     4321 tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg
     4381 gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg
     4441 tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata
     4501 acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac
     4561 gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca
     4621 tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag
     4681 taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat
     4741 tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag
     4801 aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat
     4861 ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg
     4921 gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag
     4981 gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag
     5041 cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag
     5101 ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag
     5161 gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat
     5221 tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta
     5281 cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata
     5341 tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga
     5401 cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag
     5461 gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg
     5521 cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt
     5581 cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa
     5641 gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt
     5701 aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt
     5761 tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt
     5821 gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa
     5881 aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat
     5941 catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta
     6001 acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag
     6061 aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa
     6121 gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat
     6181 ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa
     6241 tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt
     6301 ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc
     6361 ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc
     6421 cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta
     6481 gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat
     6541 gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact
     6601 cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa
     6661 atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga
     6721 taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg
     6781 aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa
     6841 aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca attattgccc
     6901 acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag
     6961 acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg
     7021 gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca
     7081 tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg
     7141 acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat
     7201 tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac
     7261 cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag
     7321 cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa
     7381 gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta
     7441 ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat
     7501 tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa
     7561 tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg
     7621 taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg
     7681 gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg
     7741 tggatcatca agatgcgtta cctatccttc acattgacga accaacattg caaatgactt
     7801 tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga
     7861 ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa
     7921 actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg
     7981 aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag
     8041 aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat
     8101 atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc
     8161 atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt
     8221 attcaactga attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc
     8281 aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga
     8341 tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct
     8401 tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata
     8461 atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta
     8521 aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat
     8581 tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa
     8641 ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat
     8701 gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt
     8761 ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt
     8821 aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc
     8881 acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag
     8941 cgggaagatg aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca
     9001 aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg
     9061 aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt
     9121 tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat
     9181 acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga
     9241 aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga
     9301 ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg
     9361 tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga accagctaga
     9421 tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc
     9481 tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga
     9541 acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga
     9601 atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt
     9661 agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg
     9721 tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct
     9781 gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat
     9841 ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag
     9901 agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa
     9961 gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt
    10021 aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta
    10081 ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat
    10141 agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa
    10201 ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc
    10261 tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac
    10321 gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta
    10381 tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta
    10441 tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga
    10501 agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt
    10561 attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat
    10621 gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc
    10681 tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa
    10741 tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta
    10801 taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg
    10861 atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac
    10921 agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc
    10981 ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag
    11041 ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa
    11101 atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa
    11161 ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga
    11221 tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat
    11281 cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata
    11341 aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga
    11401 cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc
    11461 tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt
    11521 acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg
    11581 tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt
    11641 ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca
    11701 attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct
    11761 ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc
    11821 gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca
    11881 acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc
    11941 agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag
    12001 aactgggagt gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca
    12061 ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg
    12121 gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt
    12181 tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga
    12241 tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat
    12301 aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa
    12361 atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat
    12421 gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga
    12481 caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa
    12541 gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt
    12601 gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat
    12661 atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt
    12721 ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac
    12781 ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct
    12841 cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc
    12901 tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta
    12961 cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt
    13021 cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa
    13081 cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca
    13141 ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc
    13201 gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa
    13261 ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga
    13321 attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac
    13381 tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt
    13441 ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta
    13501 gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc
    13561 ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc
    13621 acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca
    13681 ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa
    13741 attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca
    13801 cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat
    13861 gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg
    13921 gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat
    13981 ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg
    14041 tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg
    14101 ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca
    14161 aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca
    14221 gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg
    14281 gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat
    14341 actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg
    14401 gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg
    14461 cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca
    14521 atccatctgc atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca
    14581 aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag
    14641 cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg
    14701 attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg
    14761 tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga
    14821 cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa
    14881 atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca
    14941 tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa
    15001 gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa
    15061 ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa
    15121 ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa
    15181 gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc
    15241 tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat
    15301 gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat
    15361 gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat
    15421 atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc
    15481 aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga
    15541 gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat
    15601 aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc
    15661 gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg
    15721 tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga
    15781 aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac
    15841 cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc
    15901 aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa
    15961 aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat
    16021 ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca
    16081 tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc
    16141 cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt
    16201 tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt
    16261 atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa
    16321 caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat
    16381 atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat
    16441 caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag
    16501 acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac
    16561 atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa
    16621 aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga
    16681 agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg
    16741 acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg
    16801 acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc
    16861 aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg
    16921 gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat
    16981 cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc
    17041 ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat
    17101 cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt
    17161 gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta
    17221 cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg
    17281 aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat
    17341 aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa
    17401 aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa
    17461 tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt
    17521 agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac
    17581 agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga
    17641 acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg
    17701 atttttgatc attgtccaag tatttttttg gtatactagt accagtacca tttctaagga
    17761 gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag
    17821 gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat atgattggac
    17881 catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg
    17941 aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa
    18001 aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac
    18061 ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt
    18121 ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac
    18181 tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc
    18241 cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa
    18301 ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc
    18361 ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt
    18421 aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac
    18481 attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca
    18541 attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag
    18601 tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga
    18661 attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt
    18721 aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga
    18781 agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga
    18841 ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag
    18901 aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata
    18961 aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag
    19021 ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg
    19081 gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg
    19141 cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg
    19201 tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata
    19261 cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa
    19321 aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa
    19381 cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc
    19441 ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg
    19501 gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa
    19561 acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg
    19621 gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt
    19681 tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa
    19741 ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac
    19801 cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa
    19861 ttaaagaggg gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca
    19921 cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg
    19981 ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc
    20041 attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa
    20101 attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt
    20161 atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc
    20221 gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg
    20281 tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt
    20341 ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc
    20401 ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc
    20461 tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca
    20521 gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact
    20581 tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga
    20641 aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac
    20701 agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac
    20761 gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga
    20821 cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa
    20881 aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg
    20941 ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct
    21001 atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac
    21061 tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta
    21121 tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata
    21181 tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca
    21241 tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt
    21301 tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca
    21361 gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc
    21421 aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac
    21481 gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt
    21541 tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc
    21601 gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga
    21661 tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca
    21721 tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga
    21781 aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt
    21841 gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga
    21901 acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca
    21961 aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga
    22021 tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt
    22081 atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg
    22141 cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt
    22201 catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg
    22261 caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc
    22321 tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg
    22381 gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata
    22441 attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa
    22501 agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat
    22561 cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt
    22621 tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg
    22681 gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt
    22741 attatttgtt tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac
    22801 taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc
    22861 tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat
    22921 ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg
    22981 gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt
    23041 tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc
    23101 atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca
    23161 agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa
    23221 ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt
    23281 cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg
    23341 gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg
    23401 gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt
    23461 ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga
    23521 ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga
    23581 tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa
    23641 tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat
    23701 ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat
    23761 tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg
    23821 gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg
    23881 gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt
    23941 taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag
    24001 ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt
    24061 cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc
    24121 cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc
    24181 aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa
    24241 caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt
    24301 aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac
    24361 ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa
    24421 atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt
    24481 ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta
    24541 ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat
    24601 actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt
    24661 gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca
    24721 attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc
    24781 ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc
    24841 gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat
    24901 caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat
    24961 atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac
    25021 gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa
    25081 ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt
    25141 gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa
    25201 ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc
    25261 aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag
    25321 ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt
    25381 ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt
    25441 ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt
    25501 cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg
    25561 aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat
    25621 ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca
    25681 ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat
    25741 tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca
    25801 gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa
    25861 agacgctatt gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa
    25921 acaaagtttg aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc
    25981 aaagtttttt ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac
    26041 tgcagttatt ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta
    26101 ttttaatgta ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac
    26161 ggttccattt gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt
    26221 ttgccaaata gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga
    26281 tcccagtcag catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa
    26341 aagtttccac aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt
    26401 ccattttgat attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag
    26461 tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt
    26521 ctcattggca cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag
    26581 gagcatcccc tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta
    26641 tcaaatcagg aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc
    26701 agccgattgt tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt
    26761 atatcacgga tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc
    26821 tattttccta aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt
    26881 ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca
    26941 gtgatttgtg gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc
    27001 ccaccgcaaa aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt
    27061 agaccgattt ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca
    27121 atattgattc ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc
    27181 ataatcaaaa caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca
    27241 agtaaaataa ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga
    27301 aacaatcata acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat
    27361 tcataagctg tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga
    27421 cagaagacag ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa
    27481 gatcaggtaa aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt
    27541 cgtgtcctac aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca
    27601 agagaataac aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag
    27661 atagccgatg actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga
    27721 tatccatagc ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag
    27781 caggcgcatc attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt
    27841 ttttgactag ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta
    27901 cttgttcagc aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga
    27961 tgttttggga tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca
    28021 gacctaaaac agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat
    28081 tttccaaacg ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat
    28141 atgcgtgctt gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag
    28201 taatttcttc tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca
    28261 aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg
    28321 ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg
    28381 ttcctgtttt atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc
    28441 ctttgattaa aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag
    28501 cagatgcgac aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc
    28561 tttcatccca actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa
    28621 gcaaaatcaa ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg
    28681 gcgtattctg cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg
    28741 tttttgttac tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat
    28801 cgcctaaata tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg
    28861 cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca
    28921 agttatccac ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata
    28981 attgggcttt ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag
    29041 ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa
    29101 agtaattacc gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca
    29161 gtgtccgatc attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga
    29221 taatggcact gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac
    29281 cgaagatcat aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca
    29341 tcgttattag acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa
    29401 taacagctaa atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt
    29461 attatttata atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag
    29521 aagtgttctg gttttttcta cattgcaata aaccaataac caatcaaaat gataccaagg
    29581 ataatacgat accagccaaa agcggtaaag tcgtttttct tcaaatagct tagtaagaat
    29641 ttgatagcta agacggaaac cacaaaagat acgaatgttc ctacaagaag aacaacagtt
    29701 tcttccattt tgaatgaatt cccttcagct aaatatttga cgatcttcaa gccgcttgcg
    29761 ccaaacatga caggaatccc taagaaaaat gaaaactctg tagcgacaaa acgcgaagca
    29821 ccaatcaaga tagatcctag gatcgttgcg ccagaacggg atgttccggg aacaagtgcc
    29881 aagacttgga ataacccaac aagaagagct gcttggtacg taaagtcatt cagagaagtc
    29941 caacgaggtg ttttgttttt gttgcgtttt tcgacaataa taaaggcaat accgtaaacg
    30001 atcaaaacaa tggcaactgg taagaacttg tggaaatttg cttctaacca atcatccaaa
    30061 ggcaaaccaa tgatgatggc tggtatgcaa gcaacgatca ctttaaacca aagtgtccac
    30121 gtatcttttt tttctaccgt tgatttactt ggagaaaatg ggtttaactt gtgaaaataa
    30181 agaacgacga cagccaagat cgcccctaat tggataacga cattgaacat cgacataaat
    30241 tgtgaactgg catccagttt gatgaattca tctgctagga tcaaatgacc agtactactg
    30301 atgggcagcc attccgttat tccttcaata ataccgagaa tcactgcttt gataatattt
    30361 gcaaaaaaca taaattcctt ccttcccaat taagatacat cttatagtat accgttattt
    30421 tttgagaaac ccaatctgtt ttttaatatt aagaaaaact tgagaaataa agcagataag
    30481 tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt atgattctct
    30541 ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg actatgtgtt ttcccgttat
    30601 cgatataaga cgtatcattg atgccgtgga tagaaaatcg accgtttgca taagtgacct
    30661 ttgttacgct gccatttaaa agcccagcac gaacaggctg tttaggatcg atcagattaa
    30721 gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac atttccacca ccattttttt
    30781 cgcagtgatg ggcgatatct tcaaatcctg tccatacacg gtctcgcaat gtttcataag
    30841 tttccgccca cccagcggta tctaagttgt atacagcatt agcaatttgt tcaaaagaaa
    30901 cttctgtggt aaacatttca tcatatgttt taaaattcaa tacacgaggg atcacacccc
    30961 acatttccat gtcatatcca ccttcaagag acccgaaaca ccattctcta atcctttttt
    31021 caataaaata aggaatttct tttcccttag aatgttctcc tagaataata cgggcagttt
    31081 cgattgcacg accactgtca ctagaataag caaatgaaaa gtcgacattt tttaacccgc
    31141 gacctaaata atgaatgcct tcttgtcctt gctttgtgag gggtgtatca caccagcctt
    31201 gtactcgttc aattgtattg aacatcgttt ttccgtgtcg tacgatataa agtgtagttt
    31261 ctgtcatttt attctcctcc taagtcgttt taacgattga aaaaaggatt tgtccttttt
    31321 tcctgttcaa tcgtcgttgc atcgccatgt cctggataga ctgcaaattc atcaggtaat
    31381 acaaacagct gggtccgaat actatgaagt aattgttcta aattccctgt atacagatcg
    31441 gttctaccga tacttcctct aaacagggcg tcgccagaaa cgacaaaatc atcaaaaata
    31501 aagctgacgc ttcctataga atgccctggg gtagggacga cgcgaaaaga catgtttcct
    31561 aaagtataag ttttcatttc aaattcatat tctgcaggcg agacaataac atcagcaata
    31621 tcatcatgtc taccgagtcc agaaagattc aaaatcggat cacctagcca tttttgctct
    31681 agtgggctga cataaactgg gatttgataa tacgcgcgta aaggttccac tgcaccgata
    31741 tgatcgtagt gagtatgggt aagcaaaatc gctaccggct gttggtttgt ttttttgatt
    31801 tcttgtttga ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc ttcttcgttg
    31861 tagaccaaat aacagttttc ttcaatcatt cctgttttta atctttcgat atgcatgtgc
    31921 attcctcctt gatagccaaa acttctttca tccattagta taacgcattc attgctagga
    31981 aacaaaaaca ggtacttatt tctctttact tatctgaatt atcagactat atgaaaagta
    32041 tttggatatt ctaaagagaa ggaaagtaag ttttcatagc attttaatat gtccttgtta
    32101 cattagattc atccaaaaat ccaaattcta tatcctatat ccctgcctaa tcagcagggt
    32161 gtttttttat gtttggaaag agagctcttt ttgctaaact aggttttttc aggtaatctt
    32221 agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg gcacttagta
    32281 gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca tactgcatta
    32341 gaaggagcga gaatccaccg caagctccaa aaagatgcgg gagaaaacta tgaaaaagaa
    32401 gtctttttaa aaacaacagt tcaattagat gaatattacc tgatcgttga aggccgtgca
    32461 gacggtattt tcaaaagaga cggtatttat tatatagatg aaatcaaaac ctccgaacca
    32521 cgatttgaag atttagaacc agaacaagtg gaacttttct ttcatcaagc aagggtgtat
    32581 gcctatatct atagtcaaga acatgattta caagaaataa atctgcaatt gacttacttc
    32641 cagacggcag aagaagtgat cacgagaaag gttgaacatc agacaagaga gcagttagaa
    32701 acttttttta aaaagctgac tgaagattat aaagaatggc ttgtttttca agaaaactgg
    32761 cgcactgtac gcaatgcctc tttgatggct ttgaaattcc cccatgatga gtataggaaa
    32821 ggtcagcgtg aactagcggt agctgcttat aaaacgatcc gtacgaaaca gaaattgttt
    32881 gtagaagcac ctaccggaac aggaaaaaca atctctacgt tattccccgc tttgaaagct
    32941 gtaggggaag aagagggaga aaagatcttt tatctaacgg ctaaaacaat aactagacaa
    33001 gtagctgaag atgctatgac agcattaaaa gatacaggag ctgaagtaaa aagtgtcact
    33061 ttgacggcga aagacaagat ctgcttttta gatgagacga cctgtaatcc agatcagtgc
    33121 ccatatgcca atggttatta caatcggatt aatgaaggtt tatgggatct tttgaatcat
    33181 gagaatcaga tcactcgtga agtcatcgaa acttatgcaa gaaagcatac actttgtcct
    33241 ttcgaactat cattagatgt cagtatctgg tgtgacgtaa tcattgggga ttacaattat
    33301 ttatttgatc caactgttta tttaagacgt ttttttgaag atgaaaaaaa cgaggactat
    33361 ctgtttttga ttgacgaagc acataatttg gtgaatcgtt cccgagagat gtattcggca
    33421 gaactatctt atgaaaaaac gaaacgaagt aaggaagcta taccaaagga attcaaaaaa
    33481 cttcatcgcc gtttcaacaa actattaaaa gaatttgatt cgatccgaga aatagcaaaa
    33541 gaagatcatt gggattatca tcatcaaaaa gcaccggctg aatctttagt aaaggcaggt
    33601 tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca cccatcacaa
    33661 gaacaattgc ttccttatta ttttgatttg cttcattttt tgaaggtcag tgaattttat
    33721 gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt aaaagagttt
    33781 tgtattgatc caagcttgtt tcttgagcag tcgttggata aaggaaaaag cagtattctt
    33841 ttttctgcta gtttttcacc actctcttat tatcaggaaa cactgggggg acaaaaaagt
    33901 ctggcttaca aactgccgag tcctttccca gaaggaaacc agcaagtttt catcgcaaat
    33961 tacctagaga cgacttatcg aaaaagggaa cacagtctgg caaaattagt agagacgatt
    34021 catgcttttg ctgatggaaa ggtcgggaat tatttcgttt tctttccttc ttatcaatat
    34081 ttagatcttg cggtggaagc attcagacag caatatcccg acagcaatgt gctaatccaa
    34141 gaaacggaca tgaatgagga agaacgcgaa cgatttttaa caaaatttca aacaaatccc
    34201 gctgaaacat tgattggatt ttgcgtactt ggcgggattt tttcagaagg aatcgatttg
    34261 agaggggacc gattgatcgg cagtatgatc gtaggtgtag gtttgccgca gatgaatcat
    34321 gagcaggaac tgatcaaatc atattatgat gagaaggaac accttggatt tgcttacgct
    34381 tatcagcttc ctggaatgaa caaagtcttg caagctggtg gtcgagtgat tcgtggcatg
    34441 gcggatcaag gaatcgttgt attggcagat caacgatttt ctagtttttc ttatcgacgt
    34501 ttattcccac aacattggca aacagcaaga gaagtacgaa gtgttgggga attggaagaa
    34561 ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga ggaatgacag
    34621 acgtcagcct cgttttttga ttaacgatag ttagtgaatt gcaactcgat tggtaagtca
    34681 agtttacgta ataaagcaat cacagcttgt agatcatcac ggttcttacc tgtcactcta
    34741 atctgatctt cttggatttg tgcttttact ttcagttttt catttttgat agctgtagtg
    34801 attttcttcg cgttttcacg atcgattccg ttgaccaaat cggcagtttg tcgagctttg
    34861 ccacctagag cgtgttctga ttcggaaaaa tggatatttt tgattggcac atttcttttg
    34921 atcagcttac caaataatac atctttgatc tgttcgattt tgaaatcatc atcgctcaat
    34981 acaactagat tgctgttctc taatttgatt tctactgtag aatccttgaa gtcaaagcga
    35041 ttggacaatt cttttgttgt ttgctgaatc gcatttttta cttcttctgt attgacttct
    35101 gatacaatgt cgaagctagc atcttttgcc ataggaaccc tcctgatata catataaata
    35161 tttaccatac atctatctta tcataggatt agggcagatt cttacctgtt tgctaaaatt
    35221 cttctcattt gagttcgaag tattttattt taatagataa actgttattc ttacatttgg
    35281 aataccaaag taggggagag gtgcttgtga gtaaaattat gactgcgtat cataaaaatg
    35341 aaagattaaa gaaaatagtg gttattttga taaccggatt gaccgctgcg gtcgctttaa
    35401 attatttttt gattccagcc aatgtctttt ctgcagggat gaatgggatc gctcaaatca
    35461 tttcctcgct tttatatgaa tggtttcata tcaagataga cacaggaaca tttatttttc
    35521 ttttgaatat ccctgtgttt gtattagggt ttctcaaagt aggaaaacga gcaacgatcc
    35581 ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca gtcggacaga
    35641 tcactgataa tattttgatg aatgccctag taggcggtgt tttattaggt cttggcgtag
    35701 gattatcttt aaaaatgggc tttacaactg gtgggatgga tatcatctct cttgtacttt
    35761 cacaaacaac tggaaaaaca gtcggaaact acatgctgat tctaaatggt atcattgtag
    35821 tcatcgcagg cttcttcttt aactgggaaa gtgccttgta tacgattatt tccatttatg
    35881 caatgactca agtaattgat gccatccaca cgagtcatca aaaagtaaca gcaatgatcg
    35941 ttaccgtcaa gccagaagag gtaacagaag aactagcaaa acggatggta cgtggaatga
    36001 cattactgcc gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc atgatggtca
    36061 tcacccgcta cgaaatgtat gatcttgatc agatcgttca tgaaatcgac gacgatgcat
    36121 ttatcaatat tttaccaact cattcagtat ttggccggtt tgcaaatgaa aacgaacaaa
    36181 aaatgtatcg aacaactggc gtattgccag aaataaaaaa tcgttcacaa aaaaaatgag
    36241 gaatcaggtt tattttagaa taaatttaat ctattacatc taataagaac atttttataa
    36301 atttaaacga gaatcttact tctaaattct catttacaag cagctaaaaa aagagtatga
    36361 taaataaaaa tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa gaacggtctg
    36421 gacaagaaat agaactaatc aaagaacgta ttcttgctgc cttggaaatg gtgattgatc
    36481 ctgaattagg aatcgacatc gtcaatttag gcttgattta tgatatagaa ttcaatcctg
    36541 aaaatggaga aacagtcatt aagatgactc taacaactat gggctgtcca ttagcagata
    36601 ttttaacaga atctatccat gacgcattaa aagaagtgcc agaagtaacg aaagcagaag
    36661 tcaaactagt ctggtatcca gcatggacga cagataagat gagccgttat gcacggattg
    36721 cgttggggat acgatagaaa tcttggctga ctagctatga gaagcaaata ggaaaaacaa
    36781 aaaagccttt tatcctttct agaagaaaag agataaaagg tttctttgtt tat
//
LOCUS       DACCZY010000010        36533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCZY010000010 DACCZY010000000
VERSION     DACCZY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.010"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="IVZ67_000407"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="IVZ67_000407"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8164508.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="IVZ67_000408"
     CDS             2863..3969
                     /locus_tag="IVZ67_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8164509.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5014
                     /locus_tag="IVZ67_000409"
     CDS             3986..5014
                     /locus_tag="IVZ67_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8164510.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5020..5499
                     /locus_tag="IVZ67_000410"
     CDS             5020..5499
                     /locus_tag="IVZ67_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8164511.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5515..6810
                     /locus_tag="IVZ67_000411"
     CDS             5515..6810
                     /locus_tag="IVZ67_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8164512.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6821..7645
                     /locus_tag="IVZ67_000412"
     CDS             6821..7645
                     /locus_tag="IVZ67_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8164513.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7810..8709
                     /locus_tag="IVZ67_000413"
     CDS             7810..8709
                     /locus_tag="IVZ67_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8164514.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8835..9704)
                     /locus_tag="IVZ67_000414"
     CDS             complement(8835..9704)
                     /locus_tag="IVZ67_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8164515.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9857..11317
                     /gene="rhaB"
                     /locus_tag="IVZ67_000415"
     CDS             9857..11317
                     /gene="rhaB"
                     /locus_tag="IVZ67_000415"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8164516.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11314..12570
                     /gene="rhaA"
                     /locus_tag="IVZ67_000416"
     CDS             11314..12570
                     /gene="rhaA"
                     /locus_tag="IVZ67_000416"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8164517.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12584..13429
                     /gene="rhaD"
                     /locus_tag="IVZ67_000417"
     CDS             12584..13429
                     /gene="rhaD"
                     /locus_tag="IVZ67_000417"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8164518.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13431..13742
                     /gene="rhaM"
                     /locus_tag="IVZ67_000418"
     CDS             13431..13742
                     /gene="rhaM"
                     /locus_tag="IVZ67_000418"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8164519.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13824..14975
                     /gene="fucO"
                     /locus_tag="IVZ67_000419"
     CDS             13824..14975
                     /gene="fucO"
                     /locus_tag="IVZ67_000419"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8164520.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15102..15860
                     /locus_tag="IVZ67_000420"
     CDS             15102..15860
                     /locus_tag="IVZ67_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8164521.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15960..16604
                     /locus_tag="IVZ67_000421"
     CDS             15960..16604
                     /locus_tag="IVZ67_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8164522.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16611..17618
                     /locus_tag="IVZ67_000422"
     CDS             16611..17618
                     /locus_tag="IVZ67_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8164523.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17634..18476
                     /gene="kduI"
                     /locus_tag="IVZ67_000423"
     CDS             17634..18476
                     /gene="kduI"
                     /locus_tag="IVZ67_000423"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8164524.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18496..19299
                     /locus_tag="IVZ67_000424"
     CDS             18496..19299
                     /locus_tag="IVZ67_000424"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8164525.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19548..20612)
                     /locus_tag="IVZ67_000425"
     CDS             complement(19548..20612)
                     /locus_tag="IVZ67_000425"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8164526.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20609..21694)
                     /locus_tag="IVZ67_000426"
     CDS             complement(20609..21694)
                     /locus_tag="IVZ67_000426"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8164527.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVZ67_000427"
     CDS             complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="IVZ67_000427"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8164528.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVZ67_000428"
     CDS             complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="IVZ67_000428"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8164529.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23943..24596
                     /locus_tag="IVZ67_000429"
     CDS             23943..24596
                     /locus_tag="IVZ67_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8164530.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24681..25586
                     /locus_tag="IVZ67_000430"
     CDS             24681..25586
                     /locus_tag="IVZ67_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8164531.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25588..26220)
                     /locus_tag="IVZ67_000431"
     CDS             complement(25588..26220)
                     /locus_tag="IVZ67_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8164532.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26540..27064
                     /gene="infC"
                     /locus_tag="IVZ67_000432"
     CDS             26540..27064
                     /gene="infC"
                     /locus_tag="IVZ67_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8164533.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27136..27336
                     /gene="rpmI"
                     /locus_tag="IVZ67_000433"
     CDS             27136..27336
                     /gene="rpmI"
                     /locus_tag="IVZ67_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8164534.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27389..27748
                     /gene="rplT"
                     /locus_tag="IVZ67_000434"
     CDS             27389..27748
                     /gene="rplT"
                     /locus_tag="IVZ67_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8164535.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28000..29337
                     /locus_tag="IVZ67_000435"
     CDS             28000..29337
                     /locus_tag="IVZ67_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8164536.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29385..31514
                     /locus_tag="IVZ67_000436"
     CDS             29385..31514
                     /locus_tag="IVZ67_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8164537.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31489..32178
                     /locus_tag="IVZ67_000437"
     CDS             31489..32178
                     /locus_tag="IVZ67_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164538.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32586..33263)
                     /locus_tag="IVZ67_000438"
     CDS             complement(32586..33263)
                     /locus_tag="IVZ67_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8164539.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33399..33593)
                     /locus_tag="IVZ67_000439"
     CDS             complement(33399..33593)
                     /locus_tag="IVZ67_000439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164540.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33643..34083)
                     /locus_tag="IVZ67_000440"
     CDS             complement(33643..34083)
                     /locus_tag="IVZ67_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8164541.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34087..34932)
                     /locus_tag="IVZ67_000441"
     CDS             complement(34087..34932)
                     /locus_tag="IVZ67_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8164542.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35081..36499
                     /gene="pepV"
                     /locus_tag="IVZ67_000442"
     CDS             35081..36499
                     /gene="pepV"
                     /locus_tag="IVZ67_000442"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8164543.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT        11503 a         6649 c         7973 g        10408 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gatgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa cagatggttc
     4201 cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac tgatcgaatt
     4261 gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc
     4321 taaagattat tcgatttttt cagttcctta tctattcaat gatgaagaac atttctttaa
     4381 ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa atttaggatt
     4441 taccggattg acctattatg attctggtca gcgcagcttt tatatggtag acggtccgat
     4501 ccatacgccg gaagatttga agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat
     4561 caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac
     4621 gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct tgtatacagc
     4681 tggacatggt ggtgtggcta aatactattc ctatgatgag catacacgtg tgccagatat
     4741 catcatcatg aatgatgcca tcaaagaaca gctaactgat gagcaggaaa aagccattga
     4801 agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag caattgaaga
     4861 agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag ataatacacc
     4921 gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta cctacaaaga
     4981 cctttatcaa aagatccaag agcaaggagc gtagctgtga tgaaaatgat tttaaacaag
     5041 gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt tttatggcaa
     5101 gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct tgttcgattc
     5161 ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa agcacattta
     5221 gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt
     5281 gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg atggaatgct
     5341 gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg atatgtttat
     5401 ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa tctaatcgat
     5461 acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa taaaatggca
     5521 atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt agggatgccg
     5581 attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat accgtttgat
     5641 gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc
     5701 gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat agctaagaaa
     5761 ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat
     5821 gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc ttctaccgcg
     5881 attggcggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca
     5941 gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac tgcttttatc
     6001 atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg aggttatctc
     6061 gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat tcatcgttgc taaaaagaat
     6121 aaataccctg ttgtcgaaaa aggacagatg gctcatgctg gaaaaataat aaaagaggcg
     6181 attcctagcc ttctattgat tgtcatcatt atcggtggta tcttgacggg gatatttaca
     6241 gcagtagaag cttcagcgat agctgtcatt tactccctgt tgattgctat gttttactat
     6301 aaaacggtaa aactaaaaga tatacctaat atgttgtttc aatcagtagc aatgtcaggg
     6361 acgatcatgt tcttattggc tacttcttcc atgatgtctt tcgccatggc gtttaccggt
     6421 attcctcagg caattagttc gttgattatg ggcgtaacgg ataataaatt tattattctg
     6481 ttattagtca atcttgtgtt attactggtg ggtatgttta tggatgtggc gcctgctatc
     6541 ttgattttta caccaatatt tttaccaatc gtcacaagtg taggggttga tcctgtacat
     6601 tacgggttgt tttcaatcat gaatctgtgt gtaggctcca tcacgccgcc agtaggtaca
     6661 ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag aggcaatgct gaaaccgctt
     6721 cttccttttt atggggttat cttagtcgtc ttgtttttga ttacttactt cccgcaaatc
     6781 gtcatgtggc tacccaatat gctgagttag gagaatagtt atgttgaaca ttacaattag
     6841 aggacatgat ctttccgaag tccaaacgat agaagatctt gcagaaaaaa caaaagaaca
     6901 agggattcat acactgcagc tcgccttagg tctttcgttt cctgaaatac cttctggcgc
     6961 aaatgagatc aattctggga tgggaaatta tgtcaaacga gttttagaaa agcaagaagt
     7021 gaccgtagga attttaagtt gctatatcaa tatgattcat ccagatttaa cgatacgaca
     7081 agagttgctg acaaagttcg agcagtatct tcgctatgct tcttcctttg gtgcgtcgat
     7141 ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa tatacggaag aaaatttcac
     7201 agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc ttggtaaaag ctggagaaaa
     7261 ataccaaatg atggtgggga tagaaccagg tttgaatcat ccggtgtatt ctttagcaag
     7321 ggtagagcag ctgatccaag cagtagattc ggactatcta ggaatcattt tagatccgac
     7381 caatttgatc acaagtagtg attatcaaga acaagtccaa ttggtagaag aagcttttga
     7441 acgttttggt gaaaagatat gtgcggttca tttgaaagat tttcgtgtcg aacaggaaaa
     7501 aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac acaaaaatca aagaaatcat
     7561 catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa acaaaagatg atgggattcg
     7621 ttatggaaga agtcttcttg aataatgtaa agaagacgtt ggttaactaa tatttagaat
     7681 ggagggggcg gggctagaag atgccacgtc tcttcttttt tataagagta tataaaagca
     7741 gctctttttg attacaaaaa acgtggtata ataaaattgt gcataagtga acaaaaggag
     7801 gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag tcaatacgat ggtttctgat
     7861 cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa ataaaacgat ctatcatcat
     7921 catgatttct atgagatcca tgctacatta aagggagaag cgacttttta tttgaacgga
     7981 cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc attccaatga tttgcatcgt
     8041 atcgtccgcc aatcttcaga tgtattcgag cgtgtctata tctttgtcac cccttctttt
     8101 ctagaaagtc gttccacgcc atggtctaat ctatcggctt gttttcaacc aatgggagag
     8161 aagaggagtc gtgtattgca aatagaacag gaagtattga agcaaaaatt atcatttatc
     8221 gaccatcctc ctgataaaaa aatttacggt gaagacataa aatatgaaca agctctggta
     8281 gaatacttga tttttctcaa tcaagttgta caaaaagaag agagtgcgca agaacagaat
     8341 gctgtagttc ctaatgagcg tgtggaacaa atgttacaat atatctcaaa aaatttatct
     8401 catccgcttt ctttagagga aatggaaaag cagttttttg tcagcaaata ccatatcaca
     8461 cgagaattta aaaagtacac tggatttacg tttcatcagt atgtaatgaa gaaaaagctg
     8521 atctatgcca agcacttgtt gcgagaatac cgcagctcta gtgctgtata tagtaaaagt
     8581 ggtttttctt cttatccgca ttttctgaaa gcattcaaga gagagttcgg cataacgcct
     8641 aaagaatttt tgaaacgtga tacacagaaa cagttggttc attatgaaca ttttgaagat
     8701 gaacaatgag aatgatcact aaaaaataac cgcagtagct gctgttgttt acatagaagc
     8761 agaaactgcg gttattttat ttttatctat atatttcaaa atattttgaa aaaatgacgt
     8821 acatattata tgaatcagct gaatttacga aagtctctcg gcgatagtcc tgtttttgct
     8881 ttgaacaggc ggctaaagta agcaaaattc tcaatcccta tttcttcgga aatcattcga
     8941 accgtaaatt ctgtataccg aagaaggtct ttagcgtaag aaagtttttt gctgatcaaa
     9001 taatcgattg gtggagaacc gataaatttg gaaaaatctt tgctcagctg atatttattc
     9061 aataagaatt gttgttctaa ttgatcaagt gtaatcgttt ctttgaaatg ttcgtctaag
     9121 tattgcttca gtttgtaaac gtaggcggga atatcctctt cttcgaaatc tagctgatac
     9181 ttttgcaaaa tcagttcatt cagcaactcg tggatagcta cagaggactg gaaatcggta
     9241 cgggcattcg tgccttgttg catgtggatc aattctgtga tcaaatggtg gatcttgttt
     9301 tctgatggat ctcctgttga atggaaggtg ttatggccgt cttttgtgaa ctcctgataa
     9361 aatgattgga cgttctgacc attcaaatgg atccaatcca tttcccatgg ttcttcgctg
     9421 atcgtttgat aacgctgata ttcttggcag tcaataaaaa ataagtctcc agcttttagc
     9481 tgttgctgat tatcattata tgtaagcgaa cctttgccag acaaagtaaa ctttatcaag
     9541 taagaaggta aatgtgctcg ttcagtatag taagggattg gtgctttgaa gtaaccgatt
     9601 tcttggatat aaaaaaataa attcctgctt ctttgtgtag gcgtcaagat cactcttttt
     9661 gagtcttccg accatgagtg gttggctttt tcccagtaat ccatgtaaac actcccaact
     9721 tgtcaagata atgcaattat ataccaaaat agtgtgaagc aaaatataag attctgtatt
     9781 atgatagcat tgtcaataaa tgataacgct ttattaataa agtgctaatc aacaataaag
     9841 taaaagaggt ggagaaatga atccaacatt tgttgccatc gatatcggtg catcaagcgg
     9901 acgactaatg aaaagtacga gggatgcaaa aggttctttg atcttggaag aagtacaccg
     9961 gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc tggaagatcg attatcttat
    10021 caaagagatt ttgattggat ttgaaaaatt aaagcagaca gggattgatc gcctttttgc
    10081 ggggatcgat acatgggcag tcgattactg tctgattgat gaacaagggg atcggataaa
    10141 tgatccgatt gcttatcggg acaagcgtac caatggagca gtagaacagt tttctgaaat
    10201 ctattctcta gataagttgt atgagcagac agggattcag atccagcctt tcaatacctt
    10261 gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa gcacataaat tgttactgat
    10321 acctgattat ttagggtatg tctttactgg taacatggtg acggagaaaa ctaatgcatc
    10381 tactatgcag ctgctgaatg cagggacaaa agaatgggaa atagaacttc ttgaaaaaat
    10441 cggtgtctcg aaggaattat ttccaccatt gacagagccg ggaacgatac taggagatct
    10501 gcaaaaagaa caattttcaa aatatgattt gccagatgtg acctttatca cagtagcaag
    10561 tcatgatacg gcgtcagctg tattaggaac gccaggtcaa ggagaacgtt ggggttatat
    10621 cagcagtggg acatggtcgc ttttagggat tgaaacaaag atcgccaatg tttctcgcaa
    10681 tgctttttct gaaaattata cgaatgaatg gggtgcccat aacacggtgc gttttttgaa
    10741 aaacatcatg gggatgtggt tgatccagga agttgcccga atgcaagagt acgagtacag
    10801 ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg ctgcagcaag tgatggaaat
    10861 caatgaccct cgatttttga atcccccaaa tatgatagaa gagattcagc aatactgttt
    10921 tgaaacagag cagccagtcc ctgtctcatc gggagctatc gctcgttgtg tgtatgataa
    10981 tcttgctctt tgttacgcac aagagctgat caaagtagaa cacttaactg gaacgaaatt
    11041 ggaaaagctg cacatcgtag ggggcggatc gaataatcgt ctactgaatc aattgacagc
    11101 agattatgcc ggaatcattg tagaagcagg acctagtgaa gccacagcaa tcgggaatgt
    11161 tgccatgcaa atgctcagta caggtcagtt ttcgaatata gaacaagcaa gaaatgtaat
    11221 caaacagtcg tttccatgtg aaacttttta cccagatgaa aataagcaaa acacgatgga
    11281 atggtacgtg aataaaatca aaggagtaat cgtatgaaca cgattgaaag aaattacgaa
    11341 caagcaaaag aaaaatatgc aactatcggt gtagatacag atgctgtact agaaaagata
    11401 caggacatca aaatttctat gcattgctgg caaggagatg atgtcaaagg tttcttgaca
    11461 ccggatggcg aattgaccgg tggaatcatg gcgacaggga attttccagg agccgcacga
    11521 acacctgaag agcttagaca agatctggag aaggcttatt cgttgatacc gggcaaacac
    11581 aaattgaacc ttcatgctat ctatttggat acagaagaat ctgtcgatct aaatgaaatt
    11641 gagccgaaac attttgaaaa atgggtagag tgggcaaaaa aagaagaaat cggtcttgac
    11701 ttcaatccga ctttcttttc gcatccaatg atgaaagacg gctttacatt ggctcatcca
    11761 gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa gaagccgcaa aatcgcggag
    11821 tactttggga aagaaactgg gcaaacaagt atcaataact tttgggtacc tgatgggatg
    11881 aaagataatc cgattgatcg ttttacccca cgggaacgtt taatggaatc actcgatgaa
    11941 atttttacag aagagttaga tgaacggtat gtacaagatg cagtagaaag caaactattt
    12001 ggtttaggcg ctgaagctta tacagtcggt tcccatgaat tttatatggg ttacgggttg
    12061 actcgtgaca aacttgtttg tctagatgct ggacattttc atccaacaga agtgatttcc
    12121 aataaattat cttcattagc tttattcagc aaaggaatca tgttgcatgt ttcccgtcca
    12181 gtgcgttggg attcagacca tgtggttttg atggatgatg aattgcagga aatcgctaaa
    12241 gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg gtctggattt ctttgatgca
    12301 acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa atacacaaaa agcactgctg
    12361 aaagctatgc tggaaccagt tgagcgattg aaagaaatgg agctggcttt tgactttact
    12421 tcaagaatgg cttatacgga agaattgaaa gatttcccgt atgccgatgt atggaattat
    12481 ttctgttaca aaaatcaagt tccagttggt ttggattggc tggaagaagt ccaggaatat
    12541 caaaaagacg tgttggaatt aagaaaatag gagtgtggct atattgaaaa aaatagatat
    12601 tttacaagcg ccttatgtga aagaaatgat cgaaacgacg tcgaatcttt atcgcttggg
    12661 atgggatgaa agaaatggcg ggaatatttc ttatttacta aaagaagaag agatcacacc
    12721 gttcttggat cctgaaaaag tgatccgccg catctcgatg atttttgatg ccagccgttt
    12781 agcaggatgc tattttattg taactggttc aggaagatat tttaaaaatg tagcgagcga
    12841 acctgccgag aatcttggaa tcgttcgagt tgctgaagat ggcaaaacac tggaactttt
    12901 gtgggggctg gaaaacgaat gccttcctac aagcgagttg ccaagtcatt tcatgagtca
    12961 tatcgcccgt ttagaagtag acccagataa tcggatcgtt atgcattgcc atgctagtca
    13021 tctattagcg atgagtttta cgcatgaatt agacgaacgt tcctttagtc gtacgctatg
    13081 gcaaatgtgt acggaatgct tagttgtttt tccagaagga atttccatca ttccgtggat
    13141 ggttccggga acaaatgaga ttggggaagc aacagctgaa aaaatgaaag aaactcgatt
    13201 ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa gacatggatg aagtctttgg
    13261 tttgatcgag acagcagaaa aggcagcaga agtctatacg tacgtcaaag cgcaaggacc
    13321 aatcttgcaa acaatcacgg acgaaaacct gtggcattta gcagatgcgt ttggtgtcac
    13381 tccaaaagca ggttatttgg aggaagttca tacgaaagca ggtgtctgac atggtgaaaa
    13441 aagctttttg catgaaagtt tacccagaaa aacaagaaga atacaaaaaa agacatgatg
    13501 aactttggcc agaaatgcgt acagcacttc gtgaacacgg ggtcatcgac tattcgatct
    13561 atcttgatcc gagtacaagt ttgttatttg gttacttagt gatcgaagat gaaaagttat
    13621 gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg gaagtacatg gaagatatca
    13681 tggagacaaa tccagatgcc tctccagtca gttgggaatt gatgccagtc tttgaactgt
    13741 agcaacaagc aactgggcaa ccggttgctt ggggttcttt cgtttttatt gtgaaaaata
    13801 atacaaatta ggaggaatag aaaatgagta atcgaatgat tttaaatgaa acatcgtatt
    13861 ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca gcagcgagga ttcggcaaag
    13921 cagctgtcat tactgacaaa ggattgatcg agcacggtat agcaacaaaa gtaacagaac
    13981 ttctagataa agcagcaatc gcttatgctt tatacgacgg gatcgtacct aatccaacga
    14041 tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc agaggcagac tgtttgatcg
    14101 caatcggtgg aggttcgcca atcgatacgg caaaagccat tgggattatt ttgacgaatc
    14161 cagagttttc agacgtcatt agtttagaag gtgttgctga tacgaaaaat ccatgtttac
    14221 ctatattagc tataccaacg acttctggaa cagcagcaga agtaacgatc aactacgtca
    14281 tcacagatga agtaaatcac cgaaaatttg tttgtgttga tcctcatgat atcccaatcg
    14341 tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa aaaattggct gcatcaacag
    14401 gtatggatgc tatgacgcat gctattgaag ggtttatcac aaaaggcgct tgggaaatga
    14461 ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca ttctttggaa gcttctgtag
    14521 atggcgatca aaatgggcga gaaaaaatgg cattaggaca gtatattgcg ggaatgggct
    14581 tctcaaatgt cggtttagga ctggtacacg ggatggctca cccgttaagt gcatggtaca
    14641 acattcctca tggcgtagcg tgtgcagcac ttttgccaac tgtcatgaaa tataacaaag
    14701 aatatactgg agaaaagtac cgtgagatcg ctcttgttct agggattaaa ggagcagctg
    14761 aaatgtcttt agaagatgta agagaagcgg cttgtggtga gatcgatcgg ttgagcaaag
    14821 cggttggaat cccagaaacc atttctgaat taggtgtaaa agaagcagat attccagcaa
    14881 ttgcagaaga tgctttgcga gatgtatgca caccaggaaa tccaagagaa acgacagtag
    14941 aagaaatcat tgctttatat caaagtttga tgtaaaaacg aagcggatct tcaacattaa
    15001 attgcctgta aagagaaatg gtagtttata tctggatatc tcttttaaat tgatggtatt
    15061 tccgctataa tttttcttat aaagaaatag gagaatatta tatggaaaat aaaaagcctt
    15121 atggaactgt attgatcaaa gcctctaaaa tactagacta tttagcggaa aaccaagatg
    15181 tctctttgca gacgatcgca aagggtgtgg gaatgacctc ttctacagtc ttgaaaatac
    15241 tagatacttt gttgatgatc ggctacgtaa ataaaaattc agagaaaaac tatcgtttag
    15301 gtgcaaagct tattcgctac gcgaataaaa acattgaaca aatcgatttg gtagagttga
    15361 ctttaccgtt tttagaacag ctgcagcaaa agatcgatga gactatccac ttaggggtat
    15421 tggacaacaa tgagatcttg tacgtgaaca aactagatcc aaaacatcaa acgatccgca
    15481 tgtcttcaaa aatcggaatc acccgaccat tatacaattc tgcgatgggc aaagccgtat
    15541 tagcagaatt cagtgaagag cagtacactg attatcttga aaagcatccg ttgatccctt
    15601 acacagaata tacgatcacc aatgcattac ggttgaaaaa ggaaatcaaa acggtaaaag
    15661 aaacccacat cgcatttgac gatgaagaaa tcgaacgtga tattttttgt ataggagcct
    15721 caatcgtgaa aaacgaacaa atcattggtg catttagtgt aagtatgccg aaatatcgtt
    15781 taacagatga gacaaaagaa caaataatca gtgccctcaa gcaaacaaaa agcgaaatcg
    15841 agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt tttcttttct gccgtataat
    15901 atgggtatag atttccaagt tataaaataa aatttctaaa taggaaatag gtggagaaga
    15961 tgaaaaaaat tgatatttta tctcgattga aaaacgccgg agtgattgcg gtggtacgcg
    16021 gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat catcaaaggt ggtctaactg
    16081 gtattgagtt gacttttaca gtccctcaag cagatcaagt aatcaaagaa cttttgtcat
    16141 tctacaaaga ccaaccagaa atagtgattg gcgcaggcac agtattagac gctgtcacag
    16201 ctcgcttggc tatcttagct ggcgcagagt atattgttag cccttccttt gatcaagaaa
    16261 cggcagagat gtgcaatctg tatcaaattc cttacttacc aggatgtatg actattaccg
    16321 agatcaaaac agcgctgaaa agcggggtag atatcgtcaa actatttcct ggaagcgctt
    16381 atggaccaag tatcatttct gccttcaagg cgccgatgcc tcaagtcaat atcatgccaa
    16441 ctggcggtgt gagtctggac aatatgaaag agtggttcga tgctggtgtc gtaacagtcg
    16501 gagtaggtgg gaacctttta gcacccgcag ctacaggaga ttttgacaaa gtaacggaag
    16561 tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag gtagctgctt atgggaaaag
    16621 tagtgacact cggagaaatc atgttgcgat tgtccacaga gcagggaacc cgtatcgatt
    16681 caagtgaacg attcgctgcc cattatggcg gaggggaagc aaacgtagca atttctttag
    16741 ctaattacgg acatgaagcg atttttgcta caaaagtacc tgataacgca ttgggaaaag
    16801 cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa acaggtatta tctggtggat
    16861 caagactagg tacttattat atggaaactg gtagtggcga acgcgccgcc tcagtgatct
    16921 atgatcgtgc cggatcaagt tttgctgaaa tggatcattt ggaatggtcg ctagaggagt
    16981 tattccaagg aatagatata tttcatgttt caggaatcac cccagctttg tctaaaaaat
    17041 ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa agctggctgc ctgatcagct
    17101 tcgatatcaa ctatcgtgga aaattatgga cacagcaaga agccagcgaa gtaatccatc
    17161 agcttttacc tttagtagat atctgctcag ctggaaaaat ggatgctctt tatttgttag
    17221 gtatcgagaa agcgccagaa gcagaagaac aacctctaat ttattattat caaaaaatgc
    17281 aggaaaagtt cccgaatatt caggtgtttt attcgaccaa gcgtaccgtt cattcagcaa
    17341 gtgaaaatga attgaagggg acattgtgga tgaaccatga atatgtggaa tcccaagtcc
    17401 atagaatcac cccaatcgtg gatcgagtag gtggcggaga tgcttttgca ggtggcgtgc
    17461 tccatgggat tttatccaat atgtctccgc aagaaatcat tgattttgct acggcagctt
    17521 ctgccttgaa gcatacgatc catggggact gcaaccaatt tagtcaagaa gaagtcgcac
    17581 aatttttagc atgcggctct ggaaaaatca ttcgatagaa ggagtagaag aaaatgcaag
    17641 aaatggaaac acgttataca cacagtccag aagatatccg tcattattca acggaacaat
    17701 tgagaaaaga atttttagta gaaaaggtat ttgaaccagg gaaaatcagt ttgacttata
    17761 cgcacaacga tcgaatgatc tttggtggag tgacacctac tgaaaaagaa ttagaaatca
    17821 cattagacaa agaactaggt gttgattact tcttagagcg tcgtgaacta ggtgtgatca
    17881 atatcggtgg accaggattc atcgagatcg atgggcaaaa agaagaaatg aaaaaacaag
    17941 acggatacta tatcggaaaa gaaacacgac atgttgtatt ttcttctcaa gatgctgaaa
    18001 acccagctaa attctatatt agttctgttc cagctcatca taaatatcca aatgtaaaaa
    18061 tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg cctgacactg aatgaacgta
    18121 aaatctacca atacatccat ccaaatgtat gcgaaagctg tcaattacaa atgggttata
    18181 cgattttaga accaggaagc gcttggaata cgatgccttg tcatacacat gaacgtcgta
    18241 tggaagctta tgtctacttt gattatgcca atgaagatac acgtgtgttc cacatgatgg
    18301 gcaaaccaga tgaaacaaaa catttagttg tagacaatga acaagccatt atttcgccaa
    18361 gctggtcgat ccattcaggt gtaggtacaa gcaattactc attcatctgg gcaatgtgcg
    18421 gagaaaatat cacctataca gacatggata tggtgcaaat ggatcaatta aaataagcat
    18481 aagggagaga aaatgatgga atttagtatg gatatgtttc gtttagatgg aaaagtagca
    18541 ttagtcaccg gtgctgtcta tggtattgga tttgagattg cacgctcttt agctacagca
    18601 ggagcaaaaa tcgtatttaa taacttgaat caagaatcag ttgatgaagg tatagcgcat
    18661 tataaagaag caggcatcga tgcaaaaggt tatgtctgcg atgtgaccga tgaagaagcg
    18721 gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt cagttgatat tctagtcaac
    18781 aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg atgcggcaga cttccgccaa
    18841 gtaatcgacg tggatttaaa tgccccattc atcgtttcaa aagcagttat tccggatatg
    18901 atcgaaaaag gcggcggtaa aatcatcaat atctgctcca tgatgagtga attaggacgc
    18961 gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa aaatgctgac gaaaaacatc
    19021 gcttctgaat acggtcaata caatatccaa tgtaatggaa ttggaccagg ttatattgct
    19081 acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg gtgaacgtca tccattcgat
    19141 caatttatcg ttggacgtac gccagcagca cgctggggcg agccaatcga tttggctgga
    19201 ccatctgtct tcttggcttc tagcgcatct gatttcgtta acggccatgt cttgtatgtg
    19261 gacggaggca tacttgccta tatcggaaaa caaccttaaa agagctttag tacactgttt
    19321 agtggagagg ttgtgaaatc agcggattga acgaataccg aaggacagct gattaaacat
    19381 catttattcg aaaaaagagg ctgggacaga agcatttaac tccaagaaat aagaaggaat
    19441 tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttt
    19501 tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
    19561 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
    19621 tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt attcgttgcg
    19681 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
    19741 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cacggcattt
    19801 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
    19861 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
    19921 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
    19981 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
    20041 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
    20101 gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
    20161 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
    20221 gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
    20281 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt caaggcagcg
    20341 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
    20401 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
    20461 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
    20521 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
    20581 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
    20641 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaagaatgc
    20701 cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca gaagattttg
    20761 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
    20821 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
    20881 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
    20941 tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg tgaacttgat
    21001 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
    21061 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
    21121 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
    21181 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
    21241 tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg taaaaaacat
    21301 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
    21361 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
    21421 tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga aaaggatttt
    21481 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
    21541 attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact gtcacgaatt
    21601 gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat tctccggcaa
    21661 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
    21721 caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat ttggtaaatg
    21781 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
    21841 ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca tccatcgctt
    21901 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
    21961 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
    22021 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
    22081 atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
    22141 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
    22201 aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa atacttcggc
    22261 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct aatccaagct
    22321 taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag gaggctaatc
    22381 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
    22441 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgcaagg
    22501 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
    22561 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
    22621 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
    22681 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
    22741 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
    22801 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
    22861 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga ttcatccatt
    22921 gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg cttcgtcaag
    22981 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
    23041 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
    23101 gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata aatcggctgc
    23161 aagctgctag tagcggctgc atcgattcca ctaggattac catgggcgat tttttccgaa
    23221 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
    23281 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
    23341 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
    23401 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
    23461 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
    23521 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
    23581 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
    23641 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
    23701 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
    23761 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
    23821 actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata ttgggggtat
    23881 atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
    23941 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
    24001 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
    24061 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
    24121 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
    24181 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
    24241 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
    24301 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttggagcgta
    24361 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta tacgattatt
    24421 acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa agtccaaaaa
    24481 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
    24541 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc atttgattta
    24601 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
    24661 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
    24721 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
    24781 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
    24841 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
    24901 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
    24961 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cgaatgccag
    25021 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
    25081 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
    25141 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
    25201 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct atttatcgca
    25261 agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca cgacaagcag
    25321 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
    25381 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
    25441 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
    25501 ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt tggatgctta
    25561 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
    25621 gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt gagataaagc
    25681 gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
    25741 cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
    25801 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
    25861 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
    25921 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
    25981 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaaac
    26041 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
    26101 gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct catcttctaa
    26161 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
    26221 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagtc
    26281 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
    26341 attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc gcttctcgcc
    26401 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
    26461 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
    26521 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
    26581 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
    26641 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
    26701 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
    26761 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
    26821 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
    26881 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
    26941 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
    27001 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
    27061 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
    27121 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
    27181 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
    27241 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
    27301 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
    27361 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
    27421 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
    27481 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
    27541 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
    27601 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
    27661 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
    27721 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
    27781 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
    27841 ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact gaacatttat
    27901 ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
    27961 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
    28021 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
    28081 atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt cggaaacacg
    28141 ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac gcatatctat
    28201 acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt tggtgccttt
    28261 ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa ggtagttgaa
    28321 cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac cgctgttata
    28381 ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt gttgccaatc
    28441 ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg ctcgattatg
    28501 gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta tgctggtttc
    28561 aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat gattgggatg
    28621 attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa aaagaaacgc
    28681 tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat ctcatggtta
    28741 gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat cttaggtttt
    28801 attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg ttataaagaa
    28861 atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc gccaatggtt
    28921 ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc tccttatttt
    28981 tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat cgtatttgct
    29041 atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc aggctcagct
    29101 attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt gttcccgtta
    29161 tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg ggttgcttgg
    29221 ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat cccaactggt
    29281 tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa cgcctagtaa
    29341 acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa cgttttgtgc
    29401 gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat aatgcgacgc
    29461 acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt ggagtagccg
    29521 caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa taatatcagt ccagaagatg
    29581 ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa ggagacgtag
    29641 caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta gcaaaacgac
    29701 aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt gtcaatgcat
    29761 tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct tctttagaaa
    29821 gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat ggggaaccag
    29881 aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg gcttctgaca
    29941 tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat gcatctattt
    30001 taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa gcaggggtca
    30061 atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat gaaatgaaaa
    30121 aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc tctttgatgt
    30181 acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc aatatcgggg
    30241 tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac ccaacatata
    30301 tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta cgggctaatc
    30361 agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta gattatgcag
    30421 tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct cctaaagaag
    30481 gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg actattacaa
    30541 atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct tatggaaatg
    30601 tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg acggttgaag
    30661 acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg attttagaat
    30721 tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta gggggcggcg
    30781 ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg attcctgaaa
    30841 acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat gttgtagaac
    30901 gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa gcgatgaata
    30961 aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa atcgatccac
    31021 agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca accgatctaa
    31081 ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg cgattaaata
    31141 aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc gaacagaatc
    31201 gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc aaggtccaac
    31261 gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct gtcgaacaac
    31321 tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa ttttatctct
    31381 gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta cagcttttga
    31441 tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt gccggaaata
    31501 tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc cgaagaaatt
    31561 tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt gtccaagcag
    31621 aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag catcaaaaaa
    31681 aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc aatcgtttcc
    31741 tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga agatgaaggg
    31801 attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc ttcgatctct
    31861 aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat cgaagcttct
    31921 gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc tttttataca
    31981 catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc aaaagagatg
    32041 aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc atataatgaa
    32101 attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg tacggagtat
    32161 gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct ttttttccga
    32221 ataaacgatg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa caaacatgag
    32281 aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct
    32341 tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat
    32401 aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    32461 tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa aacggcaaaa
    32521 tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc tgattccaaa
    32581 acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta attatgaatc
    32641 acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa tccgacttca
    32701 tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa tactcgaccc
    32761 cagtctaatt ctgaaatcgt caaacgtaac catctgaaac gcgtactata actttctagc
    32821 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    32881 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttg ttctagtatt
    32941 ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg caggatcggt
    33001 ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa
    33061 acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt
    33121 gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt
    33181 ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc
    33241 tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa tctattataa
    33301 cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata
    33361 ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgg
    33421 atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg
    33481 aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa
    33541 ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga
    33601 actactcgac acaatacaag aaaaatgctt agacggtctt tattaagaat ttacatcatc
    33661 caaagaagcg tacgcttgga ataactgatc taactccgga ttgactttgc tcagcttcac
    33721 taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt
    33781 tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgtccccg tatacttttg
    33841 gatagttgga tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt
    33901 tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg gaaaccctag
    33961 atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat
    34021 aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc
    34081 catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc atttcggaag
    34141 tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat
    34201 gaaactatga agataaatag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa
    34261 aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cccgttgcca tagcagcaga
    34321 acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa
    34381 aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg
    34441 tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc
    34501 gggatagggt agcaaaagat gattagcagc aagtaaactt aaagcagagc catcaatcac
    34561 cagccattgt tcttttgatt ggttttctag tacttgtttg aaacgttcca aactcttaga
    34621 agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa
    34681 ctggtcagta tccgtccaaa ctacaatcat tgcttctggc agatgactgt gaagagcact
    34741 ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccgctgtgta cggcagctaa
    34801 agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc
    34861 aaatgttcct ttatgtgaaa tttttggacg aggtgtgatg actcttttca ctagttgttc
    34921 gtttatcagc atctaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa
    34981 aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta
    35041 tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa
    35101 agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa
    35161 cagcgagcgc gatgactcaa aagtaacacc agatgcacca tttggacctg gacctcgcga
    35221 cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga
    35281 taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca
    35341 catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaatcaa
    35401 ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta tggcagctta
    35461 ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt
    35521 agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga
    35581 agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg gtgaaaaagg
    35641 aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg tcttgaaatc
    35701 ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt
    35761 accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa
    35821 tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat tagttggtaa
    35881 aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt
    35941 cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta
    36001 tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg
    36061 caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa
    36121 cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga
    36181 catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga
    36241 accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga
    36301 ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt
    36361 gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc
    36421 aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat
    36481 ctatcgtttg gcgaaataat taattaaaaa gaggatcaag aattgcaaat tat
//
LOCUS       DACCZY010000011        34960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCZY010000011 DACCZY010000000
VERSION     DACCZY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.011"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            156..1862
                     /locus_tag="IVZ67_000443"
     CDS             156..1862
                     /locus_tag="IVZ67_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8164544.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVZ67_000444"
     CDS             complement(2020..2922)
                     /locus_tag="IVZ67_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8164545.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVZ67_000445"
     CDS             complement(2919..3623)
                     /locus_tag="IVZ67_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8164546.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVZ67_000446"
     CDS             complement(3654..4295)
                     /locus_tag="IVZ67_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8164547.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IVZ67_000447"
     CDS             complement(4333..4647)
                     /locus_tag="IVZ67_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164548.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IVZ67_000448"
     CDS             complement(4635..5264)
                     /locus_tag="IVZ67_000448"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8164549.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVZ67_000449"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVZ67_000449"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8164550.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IVZ67_000450"
     CDS             complement(5984..6916)
                     /locus_tag="IVZ67_000450"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8164551.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IVZ67_000451"
     CDS             complement(6913..7689)
                     /locus_tag="IVZ67_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8164552.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVZ67_000452"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVZ67_000452"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8164553.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVZ67_000453"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVZ67_000453"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8164554.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IVZ67_000454"
     CDS             complement(12014..13294)
                     /locus_tag="IVZ67_000454"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8164555.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IVZ67_000455"
     CDS             complement(13313..14239)
                     /locus_tag="IVZ67_000455"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8164556.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IVZ67_000456"
     CDS             complement(14252..15535)
                     /locus_tag="IVZ67_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8164557.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVZ67_000457"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVZ67_000457"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8164558.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IVZ67_000458"
     CDS             complement(16323..17234)
                     /locus_tag="IVZ67_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8164559.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IVZ67_000459"
     CDS             complement(17231..17695)
                     /locus_tag="IVZ67_000459"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8164560.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IVZ67_000460"
     CDS             complement(17700..18398)
                     /locus_tag="IVZ67_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8164561.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IVZ67_000461"
     CDS             complement(18385..18771)
                     /locus_tag="IVZ67_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164562.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IVZ67_000462"
     CDS             complement(18918..20588)
                     /locus_tag="IVZ67_000462"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8164563.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IVZ67_000463"
     CDS             20771..21004
                     /locus_tag="IVZ67_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8164564.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IVZ67_000464"
     CDS             complement(21103..21534)
                     /locus_tag="IVZ67_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8164565.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IVZ67_000465"
     CDS             21613..22026
                     /locus_tag="IVZ67_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8164566.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IVZ67_000466"
     CDS             complement(22064..23212)
                     /locus_tag="IVZ67_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8164567.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="IVZ67_000467"
     CDS             complement(23334..23930)
                     /locus_tag="IVZ67_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8164568.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVZ67_000468"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVZ67_000468"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8164569.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="IVZ67_000469"
     CDS             27160..27591
                     /locus_tag="IVZ67_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164570.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="IVZ67_000470"
     CDS             complement(27636..28985)
                     /locus_tag="IVZ67_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8164571.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="IVZ67_000471"
     CDS             complement(29055..30218)
                     /locus_tag="IVZ67_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164572.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="IVZ67_000472"
     CDS             30347..30670
                     /locus_tag="IVZ67_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164573.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="IVZ67_000473"
     CDS             complement(30853..32202)
                     /locus_tag="IVZ67_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8164574.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="IVZ67_000474"
     CDS             complement(32271..33224)
                     /locus_tag="IVZ67_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8164575.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="IVZ67_000475"
     CDS             complement(33326..34516)
                     /locus_tag="IVZ67_000475"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8164576.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DACCZY010000012        34772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCZY010000012 DACCZY010000000
VERSION     DACCZY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.012"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(228..1115)
                     /locus_tag="IVZ67_000476"
     CDS             complement(228..1115)
                     /locus_tag="IVZ67_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164577.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="IVZ67_000477"
     CDS             complement(1112..1981)
                     /locus_tag="IVZ67_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8164578.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="IVZ67_000478"
     CDS             complement(2118..3404)
                     /locus_tag="IVZ67_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8164579.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="IVZ67_000479"
     CDS             3677..4255
                     /locus_tag="IVZ67_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8164580.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="IVZ67_000480"
     CDS             4255..5184
                     /locus_tag="IVZ67_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8164581.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="IVZ67_000481"
     CDS             5181..6917
                     /locus_tag="IVZ67_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8164582.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="IVZ67_000482"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="IVZ67_000482"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8164583.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="IVZ67_000483"
     CDS             complement(7813..8322)
                     /locus_tag="IVZ67_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8164584.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="IVZ67_000484"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="IVZ67_000484"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8164585.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="IVZ67_000485"
     CDS             complement(9623..10714)
                     /locus_tag="IVZ67_000485"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8164586.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="IVZ67_000486"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="IVZ67_000486"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8164587.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="IVZ67_000487"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="IVZ67_000487"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8164588.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="IVZ67_000488"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="IVZ67_000488"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8164589.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="IVZ67_000489"
     CDS             complement(14677..15651)
                     /locus_tag="IVZ67_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8164590.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="IVZ67_000490"
     CDS             complement(15632..17515)
                     /locus_tag="IVZ67_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8164591.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="IVZ67_000491"
     CDS             complement(17533..19758)
                     /locus_tag="IVZ67_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8164592.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="IVZ67_000492"
     CDS             19873..20907
                     /locus_tag="IVZ67_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164593.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="IVZ67_000493"
     CDS             complement(20974..21987)
                     /locus_tag="IVZ67_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8164594.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="IVZ67_000494"
     CDS             complement(22065..22253)
                     /locus_tag="IVZ67_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8164595.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22693..22821)
                     /locus_tag="IVZ67_000495"
     CDS             complement(22693..22821)
                     /locus_tag="IVZ67_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8164596.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22824..24197)
                     /gene="rlmD"
                     /locus_tag="IVZ67_000496"
     CDS             complement(22824..24197)
                     /gene="rlmD"
                     /locus_tag="IVZ67_000496"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8164597.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24308..25363)
                     /locus_tag="IVZ67_000497"
     CDS             complement(24308..25363)
                     /locus_tag="IVZ67_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8164598.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25373..26803)
                     /gene="gatB"
                     /locus_tag="IVZ67_000498"
     CDS             complement(25373..26803)
                     /gene="gatB"
                     /locus_tag="IVZ67_000498"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8164599.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26800..28272)
                     /gene="gatA"
                     /locus_tag="IVZ67_000499"
     CDS             complement(26800..28272)
                     /gene="gatA"
                     /locus_tag="IVZ67_000499"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8164600.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28272..28577)
                     /gene="gatC"
                     /locus_tag="IVZ67_000500"
     CDS             complement(28272..28577)
                     /gene="gatC"
                     /locus_tag="IVZ67_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8164601.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28683..30719)
                     /gene="ligA"
                     /locus_tag="IVZ67_000501"
     CDS             complement(28683..30719)
                     /gene="ligA"
                     /locus_tag="IVZ67_000501"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8164602.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30736..32970)
                     /gene="pcrA"
                     /locus_tag="IVZ67_000502"
     CDS             complement(30736..32970)
                     /gene="pcrA"
                     /locus_tag="IVZ67_000502"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8164603.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33125..34684
                     /locus_tag="IVZ67_000503"
     CDS             33125..34684
                     /locus_tag="IVZ67_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8164604.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6336 g        11424 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taactaaaaa gactaagaaa caataacaaa agttaacact tcttagtctt
    22681 tctttagcac gtctatttat taaagatttt agggcctttt ctcatgtttt tacgatcatt
    22741 cttaggtgtt tccatttgtt tggatcggcc tccaccttgt ttctttttga tcaattcttt
    22801 cattttttct ttttggtcca tttttattct cctttaacga atgaagcgac taattcgata
    22861 tgatgggttt gcgggaaaag atctacaggc tgaactttct tcacggcata acctgcttct
    22921 gcaaataact tagcatctct tgcaaaagtt gccggattac atgagatata gacgattcgt
    22981 tctggtgaca tttcgattgc tgattcaata aaatgatgat ctaatccttt tcgtggaggg
    23041 tcaactacga tcacagatgg tttgatccct ttttcttgcc attttttcat aactttttct
    23101 gcttttccga cttcataatg aacattttcg attttattat ttaaagcatt gaactgtgca
    23161 tcttcaattg cttctggaat aacttccatc ccatacacct tatccactct gtctgctaaa
    23221 gacaagccaa tcgtacctat tcctgaataa gcgtcgatca caatgtcttt tttatctaac
    23281 tcggcaaact cgatggctgt ttggtacaga tactctgctt gttgagtatt tacttgataa
    23341 aatgactttg ctgagattcg ataggttttt cctaatagct gatcttcgat atagtctctg
    23401 ccatataaaa ctttttcttt gtctcctaaa atcacatttg ttttttcttc attgatattt
    23461 tggatgaccg aaaccacttc tggcaattct tcatgaatag cttcagcaat ttccttggat
    23521 ttaaagaatt tttctttacg agtaactaaa acgaccatca tttcatggga ataatgacca
    23581 cgacggataa tgatgtgacg aagctgtcct tcattttttt cttcattata tcctcgtact
    23641 tggaaccgtt gcaagatatc tctgactact ttgattgctt gatcgatgac aggatcttgg
    23701 atataaaaat cttcgatagg gactagatca tggctattct ttttgtaaaa accggtggct
    23761 aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc gaatggttct
    23821 gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt cgccattaca
    23881 tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg ggctaaaggt
    23941 gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg tgattgtgta
    24001 ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac tagtatttct
    24061 acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat ttttgctacc
    24121 cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt tttctttact
    24181 atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta ctgaactcta
    24241 ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt tcagcaccgg
    24301 cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca ccgtcgatat
    24361 cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc tctaattctt
    24421 cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca aagaactcaa
    24481 tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca ccatctaagt
    24541 tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg cttgttttgg
    24601 tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt gctgcaatat
    24661 gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc ccatcatcca
    24721 atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc cctaggaaaa
    24781 acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt ttgattggct
    24841 ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca aaaatactct
    24901 ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca gcgatattga
    24961 tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt ttttttatca
    25021 cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca tagtcgttcg
    25081 ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg attccattaa
    25141 ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa cctagttctg
    25201 cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca taggcgctgg
    25261 cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt ttcaataatt
    25321 cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc ctctatcgtt
    25381 tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt cctttagttg
    25441 ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt ttgaaatcat
    25501 cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt ttgttcggat
    25561 cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg ccatttgtga
    25621 tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg gcgatcaaac
    25681 gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc agtttctcac
    25741 tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg gcatctgcac
    25801 cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc aataccattg
    25861 cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa gctggcatct
    25921 caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat ttcgggatat
    25981 caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc aaaactgttt
    26041 tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct gaaagaagaa
    26101 cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat aaagagttca
    26161 agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga agagaaatat
    26221 tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct gtaaattgga
    26281 taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc atatcagctt
    26341 cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa gaataaccgc
    26401 caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg atccggattt
    26461 ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt ggttgatcaa
    26521 attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc cggtcaaaat
    26581 gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg aattccagcg
    26641 cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg tttgtattgc
    26701 tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag ttggttttta
    26761 actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt tccctcctaa
    26821 aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg ccgcttgata
    26881 catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta atccttctga
    26941 taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga tcgttaagat
    27001 gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga atgcaactgt
    27061 tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa aatctcgttt
    27121 gatcaatgta cggacttgtc ctgctttttt gaagtaagcg tcataataac ccgcgcttaa
    27181 ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt ctgaacgtga
    27241 gttgacatat acatcatcta atgatttcac attttcagag cgataaccgt agcggatacc
    27301 atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca atgatataat aaacagccac
    27361 accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg aacggaatgt
    27421 ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc ctaagtattc
    27481 tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg caaaatcagg
    27541 aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag ctgtcaatgc
    27601 taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag atgaagcaaa
    27661 ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta cgatgccgtt
    27721 aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa ctggaatttg
    27781 tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt ttgttgggtc
    27841 ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca tagcaaattc
    27901 atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat ccatgaccgt
    27961 tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg tcaagatatc
    28021 ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt tgctttcatc
    28081 aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat
    28141 gaatgcctcg acctcaggct ctgtttcttt gattcgttca aatgttgttt ttgtcaaatc
    28201 ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag aacgattgtg
    28261 taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac tttgataaag
    28321 ccgtcttctg tttccggcac atttttcaat aattcatcgc ggttccatcc agcttgcgcc
    28381 cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg aacaccttcc
    28441 gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg gtcggtaaaa
    28501 cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt
    28561 tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt tgctctctat
    28621 tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa aaaactcatt
    28681 tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc ttctgtccaa
    28741 acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc tcctgctacc
    28801 acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt ttcgatcgtc
    28861 tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac tgttttttct
    28921 ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg
    28981 acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact gaaataagtg
    29041 actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga ctcatagtca
    29101 cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc tgctttcgct
    29161 ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc tttgctgttg
    29221 tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttgtccaa cgtcaataac
    29281 tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt atcatacatc
    29341 tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga aacaaagtgg
    29401 ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa ggctacttct
    29461 tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa ttggtattct
    29521 tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat gatgtctccc
    29581 gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat ataatcaccg
    29641 ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt catgatagct
    29701 gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat tagcgtttca
    29761 acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgat agtaaaacca
    29821 agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat ttcatagggc
    29881 aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc aatcgaatga
    29941 catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc tagcgcatca
    30001 tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa agtgtccaga
    30061 tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc ggcgttccga
    30121 ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg gacaaatgac
    30181 cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt taatttgatt
    30241 gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt tccatccccg
    30301 cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga aagtccatct
    30361 atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac acgttgatca
    30421 aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga
    30481 gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg agaatcagac
    30541 gtgatcaaat caggatattg ttcctcgatt gctactaatt ctgcgtattc tttatcataa
    30601 acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca ttggttcagt
    30661 cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc
    30721 atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa gacgttttac
    30781 tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc ttactcggac
    30841 gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc agctgttatt
    30901 ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt tatagctata
    30961 tgctgttttt ttagcgaata caggcgtttt tttaggcgta ggacgcatgc cttcgatttc
    31021 cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt gcgtccgtcc
    31081 atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc gcgtgatccc
    31141 cacatatgcg agacggcgtt cttcctccag ttcgctttct tccatcaatg ctcgagataa
    31201 aggaaaaacc ccctcttcta gaccaatcag gaatacaact ggaaattcta atcctttggc
    31261 tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt caatgtcgga
    31321 aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat cagcgtcttc
    31381 ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa
    31441 gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt ctagatatcc
    31501 gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga tcatctgagt
    31561 gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc caccgatatt
    31621 tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag aagcaaaact
    31681 gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc
    31741 aaaactgatt gaatcttgag gatttaccag cacattcaaa tacgctaaaa tatctttgat
    31801 ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat ttgctttcaa
    31861 cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc
    31921 gtaattccga tgattgcttc gaatctcttc ttgcatccgg tctacgataa aacgggtttc
    31981 gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt tttctgtcca
    32041 caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg cgctaaggat
    32101 gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg gataatcttt
    32161 ttcaaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac tttgatctgc
    32221 atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta gcgtatactg
    32281 tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt tttgataata
    32341 cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa
    32401 gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt ttgccgcaat
    32461 ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca ggctgttttt
    32521 tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat caatgttcag
    32581 ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt
    32641 ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca tcgagtgaaa
    32701 agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac gttctttcat
    32761 ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat tgacttcttt
    32821 ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac ttcctgcacc
    32881 tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac gggggttcat
    32941 cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta tcaatcttgt
    33001 taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat ttgtttcttt
    33061 ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt
    33121 ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat attttattct
    33181 aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc gattatctat
    33241 cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac agccgattta
    33301 cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa
    33361 aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc ttcgcgggga
    33421 attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg tactttcagt
    33481 tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg catccattgg
    33541 acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt taatttctag
    33601 cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat tactctttgt
    33661 tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat ttgcttcagc
    33721 aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt tatatctagg
    33781 tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag gagtacttct
    33841 aggcatctta ggttttattt atcagaatgt cttgctggct ttgccaaaat tttacaaccg
    33901 attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt tgccaattgg
    33961 ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt ttggacatgc
    34021 ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt tttccatgat
    34081 ttcttacggt gggggtcttc caggggggat tttccttccg atattgacat tgggggcttt
    34141 aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa tctacatcaa
    34201 ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag caccgctgac
    34261 agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc ctttaggatt
    34321 ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc
    34381 attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg caaggacaac
    34441 ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac gagacttcaa
    34501 ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa tcttgactca
    34561 cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg aagcaaactt
    34621 gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga agcaatcatt
    34681 ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact tgattctata
    34741 tgattctata aaaaaagaag aggctgggaa aa
//
LOCUS       DACCZY010000013        34128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCZY010000013 DACCZY010000000
VERSION     DACCZY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.013"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..1035)
                     /locus_tag="IVZ67_000504"
     CDS             complement(16..1035)
                     /locus_tag="IVZ67_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8164605.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1046..1243)
                     /locus_tag="IVZ67_000505"
     CDS             complement(1046..1243)
                     /locus_tag="IVZ67_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8164606.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1266..1559)
                     /locus_tag="IVZ67_000506"
     CDS             complement(1266..1559)
                     /locus_tag="IVZ67_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164607.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1596..1733)
                     /locus_tag="IVZ67_000507"
     CDS             complement(1596..1733)
                     /locus_tag="IVZ67_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8164608.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1735..2181)
                     /locus_tag="IVZ67_000508"
     CDS             complement(1735..2181)
                     /locus_tag="IVZ67_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164609.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2184..4370)
                     /locus_tag="IVZ67_000509"
     CDS             complement(2184..4370)
                     /locus_tag="IVZ67_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8164610.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            complement(4370..5152)
                     /locus_tag="IVZ67_000510"
     CDS             complement(4370..5152)
                     /locus_tag="IVZ67_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164611.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(5169..6926)
                     /locus_tag="IVZ67_000511"
     CDS             complement(5169..6926)
                     /locus_tag="IVZ67_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8164612.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(6901..9732)
                     /locus_tag="IVZ67_000512"
     CDS             complement(6901..9732)
                     /locus_tag="IVZ67_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8164613.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(9729..10496)
                     /locus_tag="IVZ67_000513"
     CDS             complement(9729..10496)
                     /locus_tag="IVZ67_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8164614.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(10529..13648)
                     /locus_tag="IVZ67_000514"
     CDS             complement(10529..13648)
                     /locus_tag="IVZ67_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8164615.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            complement(13665..13952)
                     /locus_tag="IVZ67_000515"
     CDS             complement(13665..13952)
                     /locus_tag="IVZ67_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164616.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(14006..14374)
                     /locus_tag="IVZ67_000516"
     CDS             complement(14006..14374)
                     /locus_tag="IVZ67_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164617.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(14420..14932)
                     /locus_tag="IVZ67_000517"
     CDS             complement(14420..14932)
                     /locus_tag="IVZ67_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP8164618.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(14943..15335)
                     /locus_tag="IVZ67_000518"
     CDS             complement(14943..15335)
                     /locus_tag="IVZ67_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP8164619.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(15335..15673)
                     /locus_tag="IVZ67_000519"
     CDS             complement(15335..15673)
                     /locus_tag="IVZ67_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8164620.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(15657..15983)
                     /locus_tag="IVZ67_000520"
     CDS             complement(15657..15983)
                     /locus_tag="IVZ67_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164621.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(15980..16309)
                     /locus_tag="IVZ67_000521"
     CDS             complement(15980..16309)
                     /locus_tag="IVZ67_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP8164622.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(16321..16584)
                     /locus_tag="IVZ67_000522"
     CDS             complement(16321..16584)
                     /locus_tag="IVZ67_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164623.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(16602..17525)
                     /locus_tag="IVZ67_000523"
     CDS             complement(16602..17525)
                     /locus_tag="IVZ67_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8164624.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(17538..18140)
                     /locus_tag="IVZ67_000524"
     CDS             complement(17538..18140)
                     /locus_tag="IVZ67_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8164625.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(18267..19193)
                     /locus_tag="IVZ67_000525"
     CDS             complement(18267..19193)
                     /locus_tag="IVZ67_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8164626.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(19198..20697)
                     /locus_tag="IVZ67_000526"
     CDS             complement(19198..20697)
                     /locus_tag="IVZ67_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8164627.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(20710..21954)
                     /locus_tag="IVZ67_000527"
     CDS             complement(20710..21954)
                     /locus_tag="IVZ67_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP8164628.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(21941..22474)
                     /locus_tag="IVZ67_000528"
     CDS             complement(21941..22474)
                     /locus_tag="IVZ67_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP8164629.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(22501..22725)
                     /locus_tag="IVZ67_000529"
     CDS             complement(22501..22725)
                     /locus_tag="IVZ67_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164630.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(22732..22944)
                     /locus_tag="IVZ67_000530"
     CDS             complement(22732..22944)
                     /locus_tag="IVZ67_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164631.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            23306..23485
                     /locus_tag="IVZ67_000531"
     CDS             23306..23485
                     /locus_tag="IVZ67_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8164632.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            complement(23478..23819)
                     /locus_tag="IVZ67_000532"
     CDS             complement(23478..23819)
                     /locus_tag="IVZ67_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164633.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(24118..24585)
                     /locus_tag="IVZ67_000533"
     CDS             complement(24118..24585)
                     /locus_tag="IVZ67_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8164634.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(25177..25464)
                     /locus_tag="IVZ67_000534"
     CDS             complement(25177..25464)
                     /locus_tag="IVZ67_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164635.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(25461..25670)
                     /locus_tag="IVZ67_000535"
     CDS             complement(25461..25670)
                     /locus_tag="IVZ67_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164636.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(25667..25864)
                     /locus_tag="IVZ67_000536"
     CDS             complement(25667..25864)
                     /locus_tag="IVZ67_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164637.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(25861..26187)
                     /locus_tag="IVZ67_000537"
     CDS             complement(25861..26187)
                     /locus_tag="IVZ67_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164638.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(26184..26465)
                     /locus_tag="IVZ67_000538"
     CDS             complement(26184..26465)
                     /locus_tag="IVZ67_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164639.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(26478..26696)
                     /locus_tag="IVZ67_000539"
     CDS             complement(26478..26696)
                     /locus_tag="IVZ67_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP8164640.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(26693..27004)
                     /locus_tag="IVZ67_000540"
     CDS             complement(26693..27004)
                     /locus_tag="IVZ67_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164641.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(27181..27495)
                     /locus_tag="IVZ67_000541"
     CDS             complement(27181..27495)
                     /locus_tag="IVZ67_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8164642.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(27495..27800)
                     /locus_tag="IVZ67_000542"
     CDS             complement(27495..27800)
                     /locus_tag="IVZ67_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164643.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(27797..27958)
                     /locus_tag="IVZ67_000543"
     CDS             complement(27797..27958)
                     /locus_tag="IVZ67_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8164644.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(27973..28332)
                     /locus_tag="IVZ67_000544"
     CDS             complement(27973..28332)
                     /locus_tag="IVZ67_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8164645.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(28329..29180)
                     /locus_tag="IVZ67_000545"
     CDS             complement(28329..29180)
                     /locus_tag="IVZ67_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8164646.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(29197..30030)
                     /locus_tag="IVZ67_000546"
     CDS             complement(29197..30030)
                     /locus_tag="IVZ67_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP8164647.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(30037..30723)
                     /locus_tag="IVZ67_000547"
     CDS             complement(30037..30723)
                     /locus_tag="IVZ67_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164648.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(30729..31475)
                     /locus_tag="IVZ67_000548"
     CDS             complement(30729..31475)
                     /locus_tag="IVZ67_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8164649.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(31468..31809)
                     /locus_tag="IVZ67_000549"
     CDS             complement(31468..31809)
                     /locus_tag="IVZ67_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164650.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(31988..32695)
                     /locus_tag="IVZ67_000550"
     CDS             complement(31988..32695)
                     /locus_tag="IVZ67_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8164651.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            32740..33219
                     /locus_tag="IVZ67_000551"
     CDS             32740..33219
                     /locus_tag="IVZ67_000551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164652.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(33460..33726)
                     /locus_tag="IVZ67_000552"
     CDS             complement(33460..33726)
                     /locus_tag="IVZ67_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164653.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(33737..33928)
                     /locus_tag="IVZ67_000553"
     CDS             complement(33737..33928)
                     /locus_tag="IVZ67_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164654.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
BASE COUNT         9214 a         7312 c         5403 g        12199 t
ORIGIN      
        1 aacgaggggc tatttttatc gttgcggaat atttaaatac cagcgtttat catgaaaatc
       61 ttgtgctccg cctttagtgt tcccttctgg atcattcgtt gcccgcatca ttacatagac
      121 tttcttatta gggaagttgc gcatgttaaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacat ctggtctata tattccatca gcttttactc gagctaattc
      241 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag cgatgtgcca
      301 accagccaca tgtaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagggtag gatttgcagc acctgctcga gttggatcaa tgactggttt
      421 atcatctgaa gttgtcggat tttcatcggt aaatccatga gccaaatcat aggctaattt
      481 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat ccctggttga ttatacggtg tgtctagtgt
      601 aagtggaata ccatatttca ttgctgaatc tctagctaat tcaacgtatg ccttgtagtt
      661 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactatttgc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      781 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      841 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tgtactgatt
      901 atttgctact tgtgagctac cttcgtttgg gcctaaatta aattcattgt taatcgtata
      961 ggcaaaccca ttaattggca ataaaaaaag agccgttaat aggctcatcg cagtaatagt
     1021 aattttcttt ttcatttgtt tcctcctatt ttttcaaatt ataagccgac acgccagtga
     1081 taacgcctaa aaatgttgct actgcattga tagtgagtac tgtcatatct gttccattcc
     1141 atccatacgc tttgcctagt gttgcaacta atacagatgc agctggcaat actgttaaaa
     1201 ctgtccattt aatgacttga taatacttat cgggtaaaat catttcttct taactccttt
     1261 cttttttacc gagatctttc tctaaataaa gttttgattt gttgtgtgtg ttctaccaat
     1321 ttttctgtat gtgtatctaa tctttcatcg tgtttcttta gttcttcatg aatcatcaat
     1381 cgatctgatt tgctcgattc taaatcttta gtcagcaaat ctaaattgtg acttactttt
     1441 gaaagagttt cagtaatctt cgagaaagat gcagtgactg gtcttattac taataaaatc
     1501 aaagaaacaa tcgcagtgat tgatcctgcg attgttcccc attctcctaa attaatcatg
     1561 tgacaactcc tttaccttaa ataaaaacgc atcacttaag atgcgctctt atctttatta
     1621 atgatttttt ctgcttcttc gtctgtaatg catagtggaa cgaatagtcg aacttgatcg
     1681 tcagtaaaac agccccaatc atacatcatt ttcacatcgc taaaactaaa catactactc
     1741 acctcccttt gaagctggat ttagttgctc tttaatttct gcaatgtctt tgctattttg
     1801 gaacgaagca agcatcgttt ttgaattgat ttgtgctaaa ccatccgctt tttctttcaa
     1861 tgcagtattt tcctgtttaa tcgctacatc gcttagcatg agtttggcgt tgagctgttt
     1921 taaatcgccg ttctcatttt ctaacgactc atacatcgct ttgagattgt ttaaatcgtt
     1981 gtgatctagt acattcacta aaacaatcca ttggttcagt ttaggatcaa acatctgatc
     2041 agcaatcgtt agtggttccc catcagcacg aattccttcg agtggtggaa catcggtaaa
     2101 tggaacagtc ataaccatat catcaagcac ttgtccagca tactctccac ctgttcgtcc
     2161 gtatttccaa atatttttca tttttattcc tcccctaccc aataattacc atggaatgta
     2221 aaccattcat ttgcagcaaa ttcgctattc gtaataattg ttcctaagtc ttttcgcacg
     2281 tacatcgctt ttgatacact acttgttcct gacggacctg taagcatgcc atgaacaatt
     2341 tttgcgacaa attcatctgg gacttcatca tcaagtggta caccaaaagc ataagctttt
     2401 ttgaacttta ctgtaccatt tacaataact tcttttccgt gtttaataaa tgttgcatac
     2461 ccatcgataa acgattgact gttgtcttta gttaatgtat aaacagtgca atcattttca
     2521 ctgatcactt tatctcctgc tatttgaata ccatcggcaa agtttttcaa accttcaact
     2581 ctctgaggat catacacatt aacagcatca ttcaagcctt tttcagtata ttcaggtgtg
     2641 acatcccaac tgtaatcatt gggattgtta ctgtctttca agccttcacc aaagtattta
     2701 aactgactaa tattcggggt tcgtgtgtcg cctttttcga tcttgagcca gtcaatttga
     2761 catgcacctg ttgttgattg gggatactgg ataatagtta accgtttggg gtagttaact
     2821 ccaaccttct gcggtgtaaa agtcagaccc catgtatcaa ctaacccttc tactggctct
     2881 aagttaccat atctataatc tccactatcc tcgttgtaaa caatgaacgt ttggataggt
     2941 ggtttggttg ccttcatggt aattgtatag gtttgaccta aaacaaattc ttcttccatg
     3001 ttagcttggt ataggctgta ggcgctagat ttgattggaa acgtaacaga cttattagca
     3061 atattctcac ccaaaggcgc tttacctagc cagtaagggt catcaagtaa gtttggctga
     3121 tatggggtgg ctgttgagcc ttcttcgatt ttgatgtcgt acaacttaaa tccgccattt
     3181 attttatccc tgtcaacaaa gctaatactc atataaaatc gatctaaatt tgtgatttga
     3241 taattaacgt tagctgtacc tttgattgtt atttctttcc ctacatcatc tgttgtaata
     3301 tttgtactat ttgcttccaa taatatcttt tctcctggtg atgtacgata caccaaacgt
     3361 aatttatcaa tagctcctgt agttccttca tcaaatcgaa ctttcgcact cagagtatag
     3421 gttttcccac tagcaagctg aggtgtatta atacgcgtaa acataataat gcttcctgta
     3481 ccatcagaag taaaatgtag cttttcgtta tccgaagtta gtgacccgtg gtaaccaacg
     3541 ttaaaatcgc tcgattttag tttggacatt aaattcggat tcccactata atcataattc
     3601 ccgaagtcga tgctgttact gtacatcttt ttcagcttgc cgaggtcgcc gatttgctga
     3661 ttggtttgat tcatcttgtc ttcaactgct ttaagattgt tttgtgccga tgtaacgttt
     3721 tgagaaacgg tatcaatctt agtttgagta gcttgtagtt gctctgttac ctcgtcaaca
     3781 gtttgatcaa ttgcttgtgt ggctccctct acaaattcat ttatttgttg gtttgccttt
     3841 tcaatcgtag aatccacatt tgttaatgct gcgttaacag catctgttgc ttcttgtttg
     3901 actccatcaa gtaattgttg aaagtctgca ataaagtagt ctgctttatc ttgcgctagt
     3961 ccgtcgattg ctgaacgctt catacggaag gtgaagccta agttatcact tgttgatccg
     4021 tctggatact cgatgtaaac ataagcttcc accataccgt tataagcttt catcgcttct
     4081 ggcaagatat atacgacctg tccattcaag aagctttcag tgatgaggtt tttcgtgata
     4141 aaagggattg gctctttcgt gatcattcca tctacttcat catagataaa catcaacaag
     4201 cgcaagttag ctcctagcaa gtccgccggt gtgccgttct gttgttcgac gttgaactct
     4261 aacgctattt gattatcgta tgatttaaat acaagccccg ttgcttgcaa atcgtagtct
     4321 ttcggctgtg taggtacttt gatagagcct tttttgatga catgcgccat tatttcacac
     4381 cttcaatctt agttattttc acgttgttgc tcattgcggg attggaaaca ttgccggatg
     4441 aaatatctaa agctctgccg tttgacatcg ttatctttcg cgcttcgatt gtcagctcaa
     4501 attcgatgaa cgtcactccc gcgtctccat tccataaatt cgcttttgta attcgcgcgt
     4561 atctttgacg tttcacttct tctacaaagt ctccgtcacc gtctgtgtag tggatacgaa
     4621 gcgtttggta acggaaagta tcatctggca gattgactga ttgtcctttt cttaattcgc
     4681 cttcaaaaag aacagaagta gtgtaaacgc gccgccagcc taacgagtta taaccctcta
     4741 ccggttcgct gttatgtgtc attttgatgt aaatttcgcc gctataacga gcaaaagcga
     4801 cgattaattt tctcgaatcg tgatccgcga ataccatcat ttcaacaaaa ctgtcatctt
     4861 caatcccgtc agggttgtct gcgccccatc cggctttggt tgcgtatttg ccgggcggaa
     4921 tgtctaaaat gttcggatat ttttgtggta gcttataatc gcgtaaccaa cgcccacgcg
     4981 cttgcataac tgtttccggt gtagcaaagc ccgcgttgct ttcagtagct aggacgtgcg
     5041 cgtcgtgatc cgagccgtcg taatggatag ccgcttgacg caataaccat ttcacagcct
     5101 cttcataact catttcttca aaaatatttg gtttgctttg aaaatctaac atttaattgt
     5161 tcaccgcctt aatagttata agtgaagtct ctcgcgcgcc ctacgccttt gcttgtcact
     5221 tttcttgttg ctgtgtcaac ttctatttta taaaacgcta attcatccgg tgtatcaact
     5281 tggctagctg tatgagagaa gccgacgtcg caaagaatca tttttactgt tccaaacgcc
     5341 ccttcgactt gttcgtgcca atgcccgcag aaataaccta ctacaacgcc ggcgcctttt
     5401 gtattcattg cgaaggtctt taatccgccg aacgaaccgt cattcggttg tcctaattta
     5461 gaccaatcga tagttaccgg actaccagat ttaaaccctt ctatcaacgt gctaatcatg
     5521 ttttcattgc gaacgggaaa cttgctgaga cctaaaggcg tatgtcctac taacgccaca
     5581 tgatagtttc tcggaacgtt cactaaccat tctccgaaag cgttaatttg tttggcactg
     5641 aaagctcccg gcgctgtatc gctgtaaccg tccacgtact tgtcgcctgt gccgccttca
     5701 taaaaatcac aagtatcgaa acggtaaatt gctgcgtttt tatctgggaa caacacgcca
     5761 ccgtacaaac cgttccaata ctcttcgaag tcagcgttgc ataacattcc ctttcgcttg
     5821 cgccacgcgg ggtcgaaaca agcgtcgtga ttccctttgc agataataac cggtttttct
     5881 tgtcctgcta ccgctgcgtt agtgaagcgt ttgagcgtgc ctaacatgga atgtcgcgcg
     5941 ctccattcat caataatgcc tatatcgctg ccgagtgaac caagtccgcc gtcgatattg
     6001 tctccgccgt aaatcatcac atctgttttg ttccctaatt tttgaaattg agggattgcg
     6061 cgccaatgtc ttaaataaga agcatctttg taaccgacgc catctatacg caagttgtga
     6121 ttatctgcgt gaatgtctgt aatgaaagaa aagttaaatt tgctgttatt aacactgttg
     6181 acaaccgtat tcaaattgcg cggcactagg tcaacgtatt tcatcgtgtc gtaatcaaaa
     6241 aagcgtgttg tttctctaat ttgtgagctg ccgactggca cttggtattt ttggtttaaa
     6301 cgatcagcta atgattcgta gtcgccttta gcttcattca gaatgttaat gatcatacca
     6361 cctggatcga tattttccag tatttcacga ttatcttcta accactgctc ccagtcattt
     6421 ttgccctgat ccatgtaatc tttgaatttt cttagcaaat cctcaaatgt ccacacatag
     6481 ccagagtcac gtaactggct tctagatatt ccagaaatga ctcgataggt aaaatcctgt
     6541 gtgctaaatt gttcactcca agttccatca ccattaagtg atcggaaact gaaatgtgcg
     6601 gtgttttcac caccccattg ccagtcaggc tcacttaagg tgtaaacaag ccttgcttgc
     6661 gctggactgt attcttgtac tttttgttca acaggttggt tttcgccaaa ttttgttgta
     6721 ttaataaaaa acggcactag gccctcgaat gtttttagtt tgccatgttc caccacttca
     6781 acaacgaact tttgcgttaa aacatcccct tgccgaattc gaaccaaatt tattccgttg
     6841 tttggttcgg tggtggatag gaccatttta tgctgcgttt ctgccatgac tatccctcct
     6901 ttagaaatcg atgtagtctc ttgcattgtg gaaatgacct gaagaagatg gataaaactc
     6961 atccataaat tgaaaatgaa gatgttctcc agtggatggt cctgttgtcc ccatcagtcc
     7021 aatttgctga ccagcagtta ccttctgacc ttttgagacg tctactcggc tttgatgcgc
     7081 gtatcctgta tacattccat cagcgtgttt aatcactgtc caatttccat accagtcaaa
     7141 gtaatttgca tcacctgcaa caatcacttc gccatccgct gaagcaaaaa taggtgtatt
     7201 aggatttcca tttacaaggt caataccgtt atgaaattct tgtgctcctg tgattggaga
     7261 agtgcgccag ccaaattcgc tcgtcactgt gattggatcc gcaatcggct tcatatatcc
     7321 ttttgataca ggaatttcca aatctttgaa cttgtcatac cattcttgtg accatgccgt
     7381 ccgttctgga tgtggatcac gtggacgttc aaagttagcc acgaatgctt gtgctgctgt
     7441 gttgatatcg gtcagattca tgaattgtgt ccatgtgtaa ggataagcgc tagttgcgat
     7501 ccattgacca tttggtgcat gccacatcaa caatttaaac tgtgccgtga ttgtgtctgg
     7561 atcatcactg atgccagcct ttgtcattaa attgatcatg tagacacgtc cgctagttgc
     7621 gccagaagaa tcggtccatt gccatacacc ataaccgaac cctggtgcgc cattgccctc
     7681 atcagcggtt ggattagcat ctgattctcc ttgtgcattt ccaagtaagg ctgcagcagc
     7741 ttgttgtgta aaaccggcac ctattgccgt tgcccaaatt tgccaatatc gtttatcccg
     7801 atcagtagtt acttctggtg ggtattgtcc gttccaaccg ttatcgttcc ctccggaatt
     7861 gcctcctcca ttagtgtcaa tttttgtacc gttaacatag agttctttta catctagccg
     7921 tccatctatg gttatatttc cttctgaaaa attgccgtca ccataaagat tatatttgcg
     7981 cttatcagca gtaacatctg ctggaatttg aaaaatagga ttccctcgat cgccaccgtc
     8041 cccagcatta atggaaaaaa tatagtttgg ctctttccag acagcaaagc cgttaatccc
     8101 tttagaacca taagtagata caattgagcc caagcgttct ccatgtacat catctagtcc
     8161 agtagacttg acttgttttt caaaagaaag ttctcccccc tctgccacta attggaaatc
     8221 tttatcgtca aatgttttta aagctacccc ttgcacgagg ataccagaca gaatgcctgc
     8281 gcgaataaaa ttagcattga aagttccatc caacgtccaa gcggtcgtgc tatcaccatt
     8341 gtgtacatct tgaattgttt gccactcacc ttttttacat tgtttgaaag atatccctga
     8401 gttattttgg accataaaaa agcgtgatct aggaatgtta ggtccatcca tataaacagt
     8461 ttcatagatt tctctactat cactaacacc agcttcaatt ccatttaccc aataaataga
     8521 accgccatta tctcctgcac ctcgcataat gtcatcttga tattttccaa tctctgtcga
     8581 ttcgtaaaat gtcattttgc tagattctaa actattaata ttattgacaa tagaagccgt
     8641 ttgttttcta acatcttgtg ttaaattatc ccctagttcg atattcgttt gaccggtaag
     8701 ccgattgaat gtagttttat aaatacgagt tttatagtga taacctttat cgtatctgtg
     8761 aatagtcact gtatttccta tcacatctcc tccagtgact tcagctttga attgtactaa
     8821 cggtctagca gaatcgatta aggttgaata agtattttta agtaaatctg ttggatcatc
     8881 tatatcatca aacactacta cggtttctcg ttttctcatt gatccatctt tttgtggtat
     8941 cccatacttt tgagttgatt ccggatcttc aagccaattt tggcctttag gcttatctaa
     9001 aggatcacca ttcgactttt tccattcaac atcagtgaat tcaattcttc taccgtaacc
     9061 gtcaccaacc tcttcgcctc gcccacgacc tatcattgaa gttgagattg agcttctatc
     9121 tatctctctt acaactgtta atgctttact accatatacg aaacgtgtat tcgattcttc
     9181 gccaatttgt tcatatactt cgatccattt atcctttatt ccatcagaat tcaaagaaca
     9241 cctaaagaca aattccatac ctaaggtttg caattctttc aacgcttctt ttacagagac
     9301 atagtaaaaa gttgcagtta ctgttggtaa cattgcttct acgtgaccaa cgcgccaatt
     9361 tccttcagta aattcaatca atcgatcaag aacatttttt aagggctgcc cactcggcct
     9421 aatatctttg atgatgtaag catctaattc atttgtcgca aatcctaacc ctgtaaactc
     9481 taatgtttca aatgggtcgc taactttagt aattcgatac aacgaaaaag acgactcgtt
     9541 ttcacgaatc gccatatatc ttgcatcctc tatttcttta tcatattttg tcgtaacgta
     9601 aagagtatct ttcattagat cactcttatc agaactaatt tctttttctt gggagacttc
     9661 aatcaaactt cttgtatttt ttcttttaat aagtttttgc aagtgatcaa agaaataaac
     9721 tgtctcactc aaattgtcgc ccccctatag aatattttaa gcttcccatt attgctagtt
     9781 atcttctgac cttgcttaag ataaaagttc tcaaaatcac tttctaaatc aatcatagaa
     9841 gtacaatctt ctccgtttac agttacctgc tcatcggaaa aatcaaaaac caacacgtct
     9901 cctgttttta ttgccgcgtc agttatcgtg atattttgtt ctccgtttgt aattttgatt
     9961 gaattattca tggataaagt aacttcaatt tttcttggtg ttataggaaa ctgtatcgga
    10021 tttccaatat agccatcact aacacactct ttcgtatact ttagtggatc cgcacagaat
    10081 acattaaaac tcgaaataat cgagttggag tctcctggaa cagtatcagt tgatgtatag
    10141 cgaccgtagt aataataatc taattcatca tgaaacctaa tttccacgtc ttcattccgg
    10201 tataaataat tcaacagttc tttgaattta aactgtagtt tttctggatc tctatcttcc
    10261 agcttatatg ttatttttag tgttcttgaa ggtattttct gatttgtaat gattgaacca
    10321 atttgtatct cttgctgttc aacttctaca gaaagcattt ctctaccttc caccgtaagt
    10381 gtttgataac catcaatcaa atcttctaaa tacattccat cgtacatcat ggcagacgtc
    10441 ggaaggaatc gtttagaact agtgagatta atggttgtat ctttgaatga gtacatttta
    10501 ttttctcgtt gatccaaaat attccctcct aaaattccag attaatatct gcaccttcgc
    10561 ccatagcttg tgaaatatcg tccataaaca atctaaacga ttgtcttcca agattgattt
    10621 taaatacagc tggtttagta gagccaccca tatttatttt atgttcaact tgtgcaccaa
    10681 tgtttttatt tgcatttttc aaatttgcag ctatatctac atcagaattt gcattgaaaa
    10741 gttccgcgat aaagtctgcc atacttccaa cagtattctg tacgtcattg aatcctcctg
    10801 tcagaccttt attcagaccg ttcataatag cctgaccagc tggaatcaat agctttctat
    10861 cgtattggat aggtcctttg tgttcacgaa tccaatcacc aatacctcca acaaaatctt
    10921 gcacagattt ccatgcattt tgtaaccctt ctagaaaact atccatgata gcttttccgg
    10981 ctgctagtaa atcgatattt ttcaagttat caaaccagcc agttactcta tcaaccgtgt
    11041 cactaacagc atttactaaa ttatcccatg cctcttgagc accatttact aaattgttga
    11101 aagtatctat agtaccttgt tttaggtttt cccatccctg aatgatgtta tctttggttc
    11161 cagtaattaa atcaccaatc caagatttga atgaagtcca aatgtcttta gcaccttgaa
    11221 ctgtattgtt gaataaatct atcgttcctt gctttaagct gttccaactt tttataagag
    11281 tttgcacgat attattaacc gtttggaaaa accattgttt tagattattc caaagttcaa
    11341 ttgcaccata ttttgtatca atccaagttt gaattatgga gaattttaga tttatccaca
    11401 tttgaattgc actgtattta atatcaatcc ataaattagt aaaaaatagt ttcaaatcaa
    11461 tccaaatcat agtcgcttga taaactactt cattccaaat attagaaata ctttgtatga
    11521 atccagtcca taaactaacg gcactattta ctattgcacc gatatattcc gtaaatatat
    11581 tttttacaga tgtccaaata tttaccactg attggacaag tgtgttccag attagatcta
    11641 aatcctcttt catttgttcg aagtcaccag taattaaatc tataataaac agtagtggcg
    11701 cagctattaa agacttaatg atttcccaag catttactat tatgttttta acctcagcaa
    11761 atatacttgt tattgctttc actatattag agaacacatc tgaaaagcta cttacaaatg
    11821 gaccaatata ttttagtata aaatcaaaac cagatttgat tttacttgta attgttttcc
    11881 atgcagtaga tatagtattt ttaaaccctt cccatttttc accagcactt gaaactaaat
    11941 catcaataac tgatatgaca ccttctttta aactattcca aatctctgaa gcagagtttg
    12001 caatacttga ccataaattt gaaaagaact cttttgttcc agtccatttg tttttaatcc
    12061 aatctgccgc tttcccaggg gcttcttgaa ctgtagtcca aacattgtct gcgccttctt
    12121 taatggactt ccataaattg ttaaaccatt ctcctgtaga tttccatgca ttctgaatcc
    12181 attctactgc cgagcttaca gcagacttga ttccctccca taagccaatc caaaagtttc
    12241 taaaatcttc actcgtattc caaagataga tgaaacctac aacgagtagt gctaccgcag
    12301 ctataaccag aaaaaccgga tttgtaagaa gacccattgc tttacttaat ccactaaaca
    12361 attttgaact ttcacttaaa gctttaaaag ctactttcac cctattgatc catataatta
    12421 ctgaaccaat cataaaaatt aacggtccta tagcagcgac aattgctcct atggccacta
    12481 ctaatttttg agttgattct ggagcactta caaatttttc tactaagccg gatatggcat
    12541 ctgctacttt tttgatggat ggtgctagaa tcttttgaat tacaatagct gctgactcaa
    12601 aagctccaaa catttgctcg atggaagaat tcatattatc ttgcatggtc cgagccatat
    12661 cgtcagctgc accatcagaa tctttcagag attttgttaa tttgcccaat gaatcaggtc
    12721 ctttatcaat caaagccatc atccctgata atgattcttg cccatatagt gttactaaag
    12781 cattttgttg ttgttcaggc gtcaggcctt caaaagcttt tttaagtaat tctacttgag
    12841 tttttaaagg tttcatttta ccgtcagcat cataaaacga aacacctaaa ttatccattg
    12901 tatcttgcat agcctttgtt ggccttgcta acctagacaa tgctcctcgc aacgttgtac
    12961 ctgcttgaga acccttaatg cctgcgtcac tcataatacc aatagctgct gcagtttctt
    13021 ccaaagaaat ccccattgaa ttagctacag gagcaacata cttcaatgct tctcccatgt
    13081 ctccaacttc agcattggta tccgcagcag cacgagcaaa tacatcagcg acatgtcctg
    13141 cttcacttgc ttctaaacca aatcctctca aagcagtagc agtattttca gaagctagag
    13201 ccacatcccc tccagataca gctgctaagt ctaaaagacc cggcattgct ttcatgattt
    13261 cttgtgcgct aaatccagca gaagctaagt tttccattcc agcagctgat tcttttgcgc
    13321 taaaagcagt ttttgctcct agatcaatcg cttgctgttt catctgttcg aatgtgtcgc
    13381 cggttgctcc tgatatagct tttacacgac tcatttgtgc ttcgaagtcg ccaccaactt
    13441 tagcagctgc tacgcctact cctataagag gagtggtaat atactttgtc attgcggcac
    13501 cagtaccttg catcacttta ccaacagcgg ttgtcatact atttgaattc ttttcaaaag
    13561 ttttaacagc atcttgtgca tctttaaaag tctttacaaa tccactatct gtggctttta
    13621 ataaggcttc aacagaaaat tgttccatga ttctcctcct ttcctcaaga gttagcttta
    13681 gttagtaagc tttggaattt tttatcttgt tttgaaagtt cggaaactcc catgattgaa
    13741 tcttcgattt tttgataatt gaagaattct tcaaaggatc gatatacagg aactgtcttt
    13801 ttgcctactt ttttctccgc ttggacttgc tgatttgccc acgctaattc gtgaatcaac
    13861 ttttctttgt caagccaaga taactgggct gcagtcatac gaatgttgta ttcatataac
    13921 gtcattcttt cgatatctga gatattggtc attcccaaat atcgaaaaga attgataaga
    13981 atttgttcgt atgccagtgc agaatctatt ccacttgttg tttttccgct tctttcaatt
    14041 tctgattcag gtttcggacc gctaactttc ccgcgttcga ctccgccaat tcttttagga
    14101 cttcatcaaa caatttttca atgtctttaa cttcatcgat gtaatcatcc aattcatcta
    14161 atgtaataga ttcatcttca gttctatttg ctatttccag aactcgtgat aacgtgttaa
    14221 cgttataaga acgtagctct ggtaagactt ttgcggagag tcccattcca aattccatat
    14281 ttccatcgac gaaaggcatc actttatcta attcacgtac aaatttagtg ccaaatttaa
    14341 acgaatattc tttaccttta atttttaatt tcaatgtttt tcatcctcct aaaataaaaa
    14401 agagagcatc taagccctct tatgctcctg tcgaagttgc tttcacggta tctttgaatg
    14461 catattgaac aacatcggct tgatcttctg tcaaggttgc ataaccatct tgaccaacac
    14521 catttactgc aaatgataaa cttaattcaa cgttatcctc tgcagcagcc gatggagtaa
    14581 attcagacac atatgcttgg taataagtag ctttgtactt atttgcatta tcatctgttc
    14641 cctgttctgc tttattaatt tcccaaattt caatgatatc cccatttaat aaagcttgtt
    14701 tcatttcatc tacatgagaa tctcctttag caactattga agtagccgaa aaatcatatt
    14761 caaccgggct taaactttga acgtttccat cttttgtcac tgtagagtct gaatctcttg
    14821 ataaaccatt ttcgtgttct gtttgaaatg ccattttcca agcggcttcc tgagtttctt
    14881 ttttcaataa gcgataaagc aaaatgacat caataccttt taatgcttcc atgttcttcc
    14941 tcctatctaa ttctaaattc aagtgtgaca accgcccgtt ttagcggtgt attcgttgtt
    15001 gtgtcgtcca tcacttgaat tccacttgct tgataattta aagcccaata atagccttct
    15061 gtggcttcta tcaatctagc ttcattaaaa agagcagatg ccatatctga caactgcttt
    15121 cgtttcttct gtaatcccca aacggataaa accacaatca cagacccttt aatgtcagtt
    15181 ttatttactt catggattgt ctgagtgttc tcaaattcca caaaaggata accaacatcc
    15241 tctaaagttt tgtaatcgta tgttttatat ccaagtttgt tttgggatat tttaaaaagt
    15301 tcatcaaaaa ttgattgatc tcttgtctta atcatcattt caccaaggct ttcatttcag
    15361 ccataaattt gactttttgg taattaaacg ctggtctaac ataaggctgt gccgacataa
    15421 atcgagtgcc atattctaca taaggtgcat aatctgctgt cggtcctaca ataccagtta
    15481 aaccagcttc taaaagattc atgtttattg atcttcgtaa gtaacctgta tccactggcg
    15541 cacctttttg cattcgttca gtcatttcag cagtattact tttcacgact ttttgaacgt
    15601 cattaagcgt tgctgctttt ttcagatgtc gcatcagctg atcgattcct ttatattcaa
    15661 gttgtgcctt catcaagaac cacctcttgc acaattaaac tatttctata tgctggattt
    15721 ctagctgttt tttgttgcca agtctttcct tcaatctcga tatagtcaaa tgtagggata
    15781 gaaaaaagag gctgcgtcct aatgaccttc gccccttctt ccacactacc aaaaatagtc
    15841 acacttctat cagtgccaat atctgtcacg tttgcctctg ttcttgttct ttctggtttg
    15901 ccttcaaccc actcaccgag atctggatca tatttagagt cagatgaacg tttaacaaat
    15961 ataatttcat ctgtaaatct catacaaatt taaacctccc tcgctttggc ttgtacaact
    16021 cttcctgatc tttacgctta aattcatcaa tctcattttg atactctgaa aaatccgagt
    16081 caggaaaagc catagataaa ccttcttgag aatatgactg cataccttct tgaccaattc
    16141 tattgaatct tttcaacgat acttcatata caactgtttc aaattcttta ggaacttctt
    16201 gcgtatttaa caaggttttc atacgctcat tcgttcttcg ctcaataact tcaagctttt
    16261 catctattgt tcctttaaga agttttttaa tatcatctgc aatcatcata tttttacttc
    16321 ctaactagca ggttgacctg tcacattgat tgaagtagtg aattctccag aagtaaatgt
    16381 gaatgttgct gacccttctg ctgcaatcgt tccatcaaaa ccaccatttt catttttggt
    16441 cactgttgcg atagctccat cacttgaagt tgctgtagta gctgcaacaa cagcagctgc
    16501 atcgctagca tctgcaggca cagctgaaat agtaaatgtt ttagtatcgc ctactttacc
    16561 ggtccatgtc ttttgatttg gcacaatacc ggtagcaggc gttacgcttt tggggaaatc
    16621 ttcccaaatg cttcatcttt tacaatcata aatccaacat ccattgttgc acgcaaagca
    16681 atcagttctt gctcaaacaa gttaactggg gttccatctt cattagttaa agtagacaat
    16741 tgggcttctt cagaaatctt aaatgaaata ttatatggga tcccataaaa catgtaatta
    16801 aagtctccag cgtaaagtgt tcctttatct aaagacttaa ggtctactac tggtaatccg
    16861 tcaattgtat tagtagagcg atcataaata aactcaacat ttgacccgac tgtttgagct
    16921 gcagaacgta attctgtacg attttttcgg tttgaaataa acgcattagg ttcgaattca
    16981 ttttctgcta acttgtcttc taaggctagg atattatcat aagtcaatcc gccttcaacc
    17041 acattccccg cactaataac tgatccgtct agtgactgag gaaatgggtt ttctttattt
    17101 aataaggcag ctgcatcaaa ttttttatag aaagcttcag caatttttgg ctgcatctcc
    17161 tcaaagaaat ctgataattt ataatttaaa tattcacgag aaaccggaag aatgacaccg
    17221 agtttttttg cagtcatcgt agcttgcatc catttaggtt tagacgtttt aattttttca
    17281 ccttcaccca cccagtatgc gcctggtcct tctgcaaagt attcaaattt cttttctttg
    17341 tcagtcattt cttcgtattt tgctaactgc atgatcttag aattttccat aacttcactc
    17401 aaaatgagcg tattatattt atcaggaatt tttccctctt tcgtttcata taccaagaca
    17461 ttatctggat cccatgtttg agcaaacatt tgcaagttca ttggtaaaag ttgtttcttt
    17521 ttcattaagt tttcctccta tttgataatt cgatttttag cagctagttt agccactgtt
    17581 tctttagtat ttttcgatgc tgtaaattgt ccaccttcat ttggtggtgt ttgtcttgcg
    17641 ttttctctct taatcaaaga agcaaagtta gtgatgactg ctacagcttg ttttgtggca
    17701 tctgcatcat cagaaacaat cagaccaagt aaatcatcat cgtgtggtaa atttgcatct
    17761 gtcagcattt tagaagcttc tttcgtcatt tcagatagtg cctgtccacg ctttaattca
    17821 gcgatttcag cttctttttg ctccaactca tgctgtagtt tttcttccgc attcattttt
    17881 gccagctttt tagcttcttc tttttttgct gctagttctt tttcccacgc tgcttttgct
    17941 ttattcgtct cagcagcgat cattttcgct acttcatcac gagaaaatgt tttgccagta
    18001 ttgttttctt cttttgcttc gggcggtgtc tcttgtgagc cagctggtag gtttccttgt
    18061 tgtccctcat caccagatcc accatctcct ggttcagaaa aaaattgtaa gttcattggc
    18121 ataaataaac gttttttcat gattaatcct ccacggttac gccgctaccc gataaatttg
    18181 accagttacg ccggtcagcc gaaaatagct ttctctttaa cgcctgtaag ctgtaagaag
    18241 gcacaataaa aagccgttaa tttgtgttaa cggcttgata ttcgttctat ataaggtgct
    18301 gtactgcatc gacaaaatgg atgcatatta ggagcgttac ttcctggctg catatcggca
    18361 acatcaaaaa cttgattatt taacggtata catagtttgc acgccgttgg ttctgctata
    18421 tagatgtact gggtaatgcc tgcatctcta taacttcgtt cttgaatccc tacctgaact
    18481 ctagtcgttt cagtcaccat caaacgttga gtgttgaact tagtgttttc tcgtccttca
    18541 gctgttaaat attctgccaa tttagatgca agttgcttgg gatttttccc catcgttata
    18601 cttctgacta acaacctatc taattctgat ttcaattcag attgatacat ccataagcga
    18661 tcactaaaag acacgtcatc actcaaaaag gagctgttta ttactagttc tatcagcttt
    18721 gcatatccac ttgaagcaat agtcatttct aaaataccgg cttgtctctt caattcagct
    18781 aaaccagctt ttgttaattc attcgaaaag tacttatcca attcattaaa cagtgaaatc
    18841 aattcaagcc cgatattagc ttttaagagc tctaatctat ttacacgcat cgtaagattg
    18901 tatagcttta attcttggtt tgctgtagga gaaaaatctt tctctttgac atacttcttt
    18961 gctttgcgag cgaatgcttt gacatccatt tcactagcac gcttcatcgc ttcgctacga
    19021 gtgattttct ggccattgga gaaactgtcc cactgcgcgt ctatttcttt ttgtatcgca
    19081 tcttgtgcgt attgcatgcg acttttaatt tctgccatgc gctttttatc atctttaatt
    19141 tgttgctttt gccattcctt ttcccgtttg atccaatatt cttgggagtt catttaatca
    19201 cccccctgtt tcatcttttt tgttgttaat tacttcttca ccatctgaat caaaaatgcc
    19261 aatctgcttt tgcgtttctt tatttactcg tttcaactct gcctgtacat ctggaacaaa
    19321 agaagcgagt cctaagatcg tctcttgact gagttcagct ccagcatcaa ccaaagattt
    19381 caactcttcc agaatggctt taggtagatt aggcgtaaat attacacgta agcctttcaa
    19441 atcggagtta tccatttcag aaatacttga ttttaggcta aataaaagac gataacgccg
    19501 cataaggcct tttttgaata gcctttgctt tgttgccgtc atttgttcaa atccaaataa
    19561 tttatatttc attgcttctc ccgattgcac tccggaaaaa ttgtcatcag taagatcagg
    19621 aaccatggat atttcgtgaa tatccttgcg tactctgtat ttgtatgctt ctacaccgtt
    19681 cacatcatat tgtttgtaaa tatatcctgc agtcacactt gttttattac cgttcacatc
    19741 agttccagac tcaagcaaaa gcatattcgc ttctttctgc ttgatggcgt cctctgtgga
    19801 tagtcctgct gcttcaatat caccactaat aactagaaga gcatcgttta gatcagtcat
    19861 ataattggcg gtatcagact gccctgcatc gtatagatca atcaaagata gtacatcttc
    19921 atacaagccc atccgaaaac gattaggaga atactctgta ataggtacct ctttatattc
    19981 atgcggttca tcctggggat tctttaactc aattgctgtt agtgtcgtct catcataagt
    20041 gatacttttt tcttttgtgt atacgattgg ttgaatgtac tgtttatcag catccttgtt
    20101 gaatcttgtc ttaggatacc gtacagccaa aataggctct cgttttactg tagtatcata
    20161 tacaacaaac gtttcaaata cattagccaa atcaacataa tctgtatcat ctgaatctcg
    20221 atagataatc tcataggctc tcccatactt atccatatca agccagagtt cagcatttaa
    20281 cccatctatg tcattattag tattaaactc ttcgatttct ttttgttgat ttgtgtcctc
    20341 gatttgcact tttataggat tgcctgtgtt gtacccaaca tcaaacgtac aaagaacttt
    20401 tccaaagtta tgtgctgatc ggtgatccgc tttttctttt tctctgcgcc tacggttatc
    20461 catgatattt gtatttctag ctttataata atcatctaat acacttagcc tttttacctg
    20521 atattcatga tgatgtttta tcattgctgc taaagtatct aaatcgttaa gtaaatcttc
    20581 tgctgagcta aatctatagt gaagatttga gtctacgcta aactttacat aatttgtgtt
    20641 cacgtcatta gaataatgta tatcagctcc gtattcaaac tcgtttacct tatccatttt
    20701 tcacactcct taaaacatgc gtttaatacg tttaatcttt tcgttgacat taacttttgg
    20761 tttcttaaga agctcatgag tatatatcgc atatcgaacg gagtctagca catcatcata
    20821 ttcttttatt ggctcgcctt ttttcttgtc ccaaatgtat tgataaattt catcccgaaa
    20881 tttcatcact ctatcttgac agataaacaa tttatcttgt ttaaatctct tggcgacttc
    20941 ctcaactcca gataaccgtg ctttatgagc atttttagca ttaatctttt ctcttacaaa
    21001 cctagccaca tgctccggtc tcgcagagtc acaataaaaa ggtactcgta agccgtaacg
    21061 ctcttgaatg ccttttgcca catctaccca ataatcaatc tcttcaaatt gagtagcatg
    21121 ttcttcgatt aaataagctg ttccgtcatc cgtttcacct ataactacga ttgaacccca
    21181 gtgttcatat ccccagtcaa caccacaata aaagttgctc aaaggtggta agtcttttga
    21241 ctggatgtag tgcttgctag catcaaaatc acgataaacg acaccttctg cagaaaccca
    21301 taaacctttg atatcacgat cataaaacat gccgcttggt gttgattctt taatattgtt
    21361 acgatatcgt tcagataaaa atgtattatc atctaattca aagtggaacg attgaatatt
    21421 tttgcttgaa ttgtcgatat actctttctt tagccagtgt tccgggttgt cagggtttgt
    21481 atcagctagt attctcgctc ctgttcctga acaacgtgaa acgatttcag caaatacttc
    21541 ttgtctagct agcgatgcct cattgatata agcgccatat gctgtcatac cacgaattgc
    21601 gccaacaccg ccaatgtttc ctgtataagc ttgtacgacc ttaacgccaa atagcttaaa
    21661 gtttccatgt ttatcgaatt taggttctat gctgtacatg ttgtatagtt cctgtaagat
    21721 gttcttttgg attgttgcac ttgaaactcc tgctaggata tacattggtt ccttaatgtc
    21781 ttcttcatcg gcaatctttc gcacacgtcg caattcaaac aagaacaaat cattgtttat
    21841 ttttgttttt ccagaacgtt ttgctccatg taataaagta ataaaccaat cattcttaat
    21901 ggttttattt aacacgtcga tttgctttgg gttataaata tcaactagtg ccatctaatt
    21961 caccactaat cttttctaac aactcatcaa gtttttcttc ggtagaacgc tctgagttta
    22021 tctgtaattg ttctaatttt gcttgcatta attttatttc tgtttgagct ttttctaatt
    22081 gcgtttgtga taacagcgat ttatacttca aatacaaatc caaggctttc aatttgcttt
    22141 ctaaatctgg tgtatattca tattgcatat gtttaataac cttgtttttc tccaagcggt
    22201 ctatttgctt gctcacgctt atttgtactt ctccttgcca aatatctatc aattcattta
    22261 aagcgtcctc ggcacttaat ttgcgcttct tttcgatagg atttagccgt tcattaatat
    22321 actgtattat gttaggttta gttaggtttt ccttacctat tactcttgct gatcgtttgc
    22381 tataacctgc tttaatggcg gcttgagtag cattaccacc attttttata tattcatccg
    22441 caaaagcttt ttgttttggt gtcaaattta ccacattcaa gccaccacct ttcaatttta
    22501 ttaacaatat ctctcaatat ttgtctgtat gttctgctca ctaaaatatc catggccgca
    22561 gtaacgaaga ttgtacttat caatctcttt tggcgtagct tctctggtca tttcaatgat
    22621 ggagtacttc tttttaatct ggactgactg gacaactcta attggatcat ctgcgtttgg
    22681 ttgcggatat ttatttgata atgatacata ccagtagttc ctcattacgt atcactcttt
    22741 ctgttggtta ctggcagaaa aggttcgcat cataaattcc atagcctgtg cgtcattaaa
    22801 tccttgcaga ataagctgat cgtagaaata cttagcttgt tttgcgatta acgctaagct
    22861 tttttgagtt tcgtagaatg tcgcctccat tgttttgttt gcttcattcg ttttaaacaa
    22921 ctctcttaat tggttttcgt tcattttttt actccccttc caaaataaaa agaccactcg
    22981 atgagtgatc taatatgtaa aaactacacc tcataaacga gatgcagtta tagttctatc
    23041 aagcaaccta caccgattcc atcgattact atcgacctcg ccttgctcgt gtactttgag
    23101 cgcccatttc caaccctcag ttgccaaagt cactggcaag gaatcgaacc ttgcatggtt
    23161 gccgaagcat tgacctaaca cacatgctta gcgtctaccc tttccgccac agtgacaaat
    23221 taatattgtg aaaataaata ctaagtgtat aattttattt atcagcgagt ggtccgctga
    23281 aataaattaa aaggtggtga aaaatttgta ctttgttata agagaagcaa ctaatggcca
    23341 atattacttc gttattaagt ccgataacaa tgaagtagta gcaactagcg aaacatactt
    23401 gactaaatat tccgctgaac aaaccattaa ttctataaaa aacggaatca ccaaagactc
    23461 tcaagttatc gatgtgacta aataagacga ctaacttcat ttgctaggga gttcatttca
    23521 gctgcttttt ctaccaaagc agttgagata ctttctagct tttctgattt aacagaatta
    23581 aatactggcg tcttgatact aatcgataca tcttgacgag gatcaggact cgttgctcta
    23641 tttttttgca ccactgatct gtacttttct actgaatgat actcaattac ttgttctaaa
    23701 tgttttttgt actctttaca tacagcttct ttttggatca attctaaacg ttgttgctca
    23761 ataatatcaa tcaaccgttc ccgatccata ccttgatact ttatttgctc gacttccatt
    23821 cgttttccct ccaatacata aattaataaa cagcaacgga tgatagataa taagaacaat
    23881 ttagaaggag ttggaattca catccttatt cttaatattt ccgttgctgt ctatcgaagc
    23941 ttaattgtga aacaataata aaacgatgtt ccttttatta ttattttgtc tcagacctat
    24001 cactaatctt tcgacactat cataataaat catttaccag gtaagtgatt ggtataaaaa
    24061 aggtataaaa tggaaaccaa atgggtaatc aaagggtata aaaagtgtaa aaactggcta
    24121 cttaaaagca accagttcta acgatgaagc aaattggatg ataattctgt ttgattctac
    24181 tttaaccgat tcttcgctag tattattcct ttgagcagtt acataaatgg gcagaccatt
    24241 gatataacga tcatagaata tcttcttgcg cctttctgtt acatcaggtt tatgcggatg
    24301 ctgaattgct gaatagccac gaacaaataa tttgtgcaag tactcaaatt cttcttgcgc
    24361 ttcctctctc tcaatcatca ttcgttctgc ttcaaatgta ttatttgctg tagaaggtgg
    24421 aaccaaggag taagatgccg ttactttagg ctcccgaggt tgacctatcc tacatctagc
    24481 tgacagatat gctgaaagaa aaacaccaac attatgtttt gtgcgatcca tatccacatc
    24541 ttttgcctct ggtgtctcat acttctttac atcgaaaagt accatccatt gattcctcca
    24601 tttatgatat aataattgtg tcagaattat taattaaggt cggaggaatc cggctttttt
    24661 tatacccatg cttatgctaa gcttttcaag tagcgaggtt gcactcgtta cccatataca
    24721 tgttgagcta tctggcggaa aacagatggc tcactatttc aatattctgc taaggacagc
    24781 cagtggtcgg ctgtcttttt tatattttaa tgagaagcct tacttatttt tttatcttta
    24841 ttacgaaaaa tgatattatc tattgataat aaagcgtata ggagatggtt gagtgactat
    24901 gtggagcatg ctgttatttt ggattcccgt ttgtattggt atcgtcgcat tttgttactt
    24961 tgtcaaacac tctagaacaa ataagctcct catgttatct tttttaccta tagtattttt
    25021 tattgtacaa attgttaaat atacctatat tgaatcgcaa gaaatattca ttttttatgt
    25081 ggtaggttta tttatctctg tggtcttttt cataatgata ctttcctatt tttataaaaa
    25141 ataaattttt ctcttagaag tacatttttt cgctgtttat atagccttca tatccaccaa
    25201 tctcgctaca gctaaattct ctttgctttt cgctaaccac ttatcacatt ccatcgtgtt
    25261 ttcaatacga atgatcgctg agtgattata gacgtgttct acatatccac gaaatggata
    25321 gatgaaccct tctgcttcgc agcgaaccat gtcaccgact ttgaattttg gtttcttacg
    25381 ttttttagga ttctttgtcg gcatatctag cattaaaccg ccgataccat gactactagc
    25441 gtaaaatccg tcttttaatt tcatctttct acctcctcat gatcgacagt gaccagttca
    25501 tatacttgtg ttaatccacc aagtcgtcgt gaaacttggt tagcttcttc gaggctatca
    25561 aaccatctcg cactttggag tatgtctaca atgaataatg tgttagcccc tatagagcgt
    25621 tcatcacttc tataaaattt attcccgaac tttactacat aaatcttcat tctgcttcct
    25681 cctcaatcgt ccaagcattt gtaaatttct ctaagcttat tagttcatct tcatttaaat
    25741 atccgtaacc atataaggat tctaaaaggt cttcttttga atagcctcta aaatcaagtc
    25801 tgagaccgct taggatgtat acataggttt tatttccata aaacttgatt gtgtatgctt
    25861 tcatcccact tcctcctcat cgatctctat ctcgacatct ctgccttcta aaatctgttt
    25921 ggtatcaata taggctacga gtatttttcg attttggctt aaagcaatat acggatcaat
    25981 caaagcaact tctgcgtgta gacgataata agcaatacaa tctatttcta aaaatatcaa
    26041 acgatcaata gcatcattaa tcataaaatc tgtaacgttt tctgctgtat ttaaaccatc
    26101 tgcatattta ggcaggactt cattattgat gaactcagct acatgttttg gacataaatc
    26161 agtcagttct tgttgtgtat agttcatttg ctgtcctcct gattaatata tttcctcaaa
    26221 ctacgtatcc ctttaccacg tttttttgtt ttgcgctcaa tccacgttcc tgtagttccc
    26281 cagtagtcaa taattccatt atgggtatta attgcaaatt gatgattgtt tggataatga
    26341 gtaaattcaa atcccaaggt ttcaatattt ttagttgctg aattgcccat ctttctgaca
    26401 tgttgttttc tacgttcttt tagatgcggt ttaatatctt tccagtattc gcccatatcg
    26461 cccatttcgc tgtcctccta tctccttgtc cacaataaaa accacatcaa ctcttctgtc
    26521 gtaccgttta aataaagatt tatttctgca ccaatcgcaa ctaaaattcc gacaacaaac
    26581 aaaataatcc ctactgctgt atattttttg ccttttgcac tctcgtaaat gaggtatgct
    26641 cctaacatcg ctagtaagcc gccaattaca caccaaaata ttgttaggct gatcatttgc
    26701 tgtcctccaa atcactcgac ttcacgaata caccatctac catttttcct gtgcgtcctt
    26761 tgatttcgtt gtatgcttgg ttcagacact cgtacaaatc catatcattt tgcatagcta
    26821 aaataatcaa ggttactact acatctccaa ttccgtctct taaaccatgt tcatcttttc
    26881 tagctagaga agcggcaact tccccgatct cttcgatcgt ttttaacatt tgcttgctgg
    26941 aatcagcttg atccaatccc ttatctttag cccactgctc tacttttgtg attagttcgt
    27001 ccattattca ttctccttta tatatttaag ttggataata caacttgtaa caaatgaagc
    27061 taatgttaaa attgtgccga ttggtgttaa aaaaccgctt tttatcgcat taattaatat
    27121 agctacaaat atcgttatat agaataaaaa gtgaattgac gctaacgttg aattaaacat
    27181 ctagtcctcc tcgaaatact cattcagtat ctctctatac ttttctacaa atttgaaacg
    27241 atcctgatga agtttcttgc tccaatttgt ttgccgatcc agctcacgca tctgatcgaa
    27301 ccctttttga atttcgttgt aataaaattc aatgtttgct gctgctttcc aatgcctgct
    27361 acttcgcact cctgctcctg tttcagccat ttccaactta actaattccg ctcgttcttt
    27421 tgctttttta tctttctgaa tcttcatcat gattttcttg aggatgatat cactgtattg
    27481 tgtaatgaga tccattattt ctcctccaca tacctaaact gtcgcccctt tgaatcaatc
    27541 cataagctcc tagctctatc ccagatgatg tttttgctca gtccagtaat ttcagataac
    27601 tgttcagctg tacccgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc
    27661 gttttccgtt taggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact
    27721 atttctttgt cttcttgcca attctcagaa tgtgtcagtt caatgattcg tttcattgct
    27781 gctttcttac ccacgctcat tcctccaatc tacgaatttc ccttcttaaa ttcactatgt
    27841 gcaaattgat tgcctttctc gccgtttcat tgaccatcac tgcctttatc cgttccagat
    27901 cgtcaatctc acgttgaagg cttcgaatac gcatttgaat cacttcttct gttgtcatga
    27961 tggaccacct cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat
    28021 caacggcact tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg
    28081 cattcctttg acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg
    28141 aatatactcg atcttgcgat acttttttcc atctagttca aatttcggca tcagctcaaa
    28201 tctttcctga agttttactt tccagccgtt cgcttctgct tgccacaagg ctaaatcgta
    28261 atactctgct tccgcgatag aatcaaactt gatacctcga tgaacagttt tcttattacg
    28321 gtatttattc atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg
    28381 aatttcagcg cataaccttc tgaattctta aatattctag aaacaattct ttcgccgtag
    28441 gcttctctta gttcaggacc ggataagttt gttgtgatga tcgttgcctt gttctgtctg
    28501 gcttctaaga gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccgcta
    28561 cctaattcag ctccaatatc gtcaaaaacc accaaatcag ttgttttgat atcggctata
    28621 agcgatcctt caatttcttt tctcagttca gcattgttat aagaaaactt tatttgctct
    28681 aataactctt gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt
    28741 tcccaagctg tcgccattga caagtggctt tttccgcttc ctgatttccc tgatagaatg
    28801 aaatgtgcag gatggttcag taagacatca tttacaaagc ttttagctct ttctaaagca
    28861 attttcgttt cttggtccac tacgtgataa ttctccattt tgcatttaaa caaagtttta
    28921 tctgttaata ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctgtcgtta
    28981 tatatccgtt cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg
    29041 catgttggtt tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt
    29101 tcgctacatt ctggacattt cccgctttgc actaatactc ttcttattag tttctccata
    29161 gcatttgcta ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc
    29221 actaggattg ctgtactgta gtttttgact ttgcttttta tgattattct tgtctgcttt
    29281 gatttcgaat ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc
    29341 aaaccaaaat tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagacgc
    29401 tttatctctt tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt
    29461 catttctttt ggaaaatctt cagttaaatt actttgcaat tttttagcaa ggcgtaagtg
    29521 ttcgtcagaa tacttacctt tcttttcttc tttatctata tctatatctt tctctatctc
    29581 tatctctaac tctggtgtag ttttgtctgg acatttgtcc gacacttgtc ctccagttat
    29641 taaattccgt tttgcctctt ctattttctt tctgtattct ctttttctat ctgcttcagt
    29701 tgaggatttt ccaatgaaac tttgtatatc agacatataa attgctccgt tatctaatac
    29761 gtcaataagc tgcaaatcac ggaaaatttg taccgctttt tctacgactc ctacagaatg
    29821 tcttgtaata gttgcgagca ttgtagagtt aaatggaatc ctgtcattaa acatcaactt
    29881 accttcgtgt tttagacttc ttaaataaag tttgagaaga atattagaat aaatatagcc
    29941 atctggcata ctttctaaga gaaccatctc gtcactatcg aaaaaattct cttttagttt
    30001 taaatagtag tagcgtttgt tgtcagacaa tattttttac cctcctattc taagtttctt
    30061 aattgtttcc tggtttaact tgatcccttt gatttgatat ttatttttga aattgatcac
    30121 acctatattg tgtttctccg tgtgatgaat cctgcagagt gctgcaaatg tgtactctga
    30181 atgatcaact tctttgcgct ttcgtcttcc tagcgctttg tcaaagtgat cgatgtcagc
    30241 tcctgttttg ccacagatgc agcagactct ttttgtgatg catttgtaga agtaatattc
    30301 ttgattcgct ggtaaaatct catagccttc tttgaaagga atatgatgtt caaagatgaa
    30361 atctaagatg atatttgcta agacattagc atcactcaca gttgtattcg attcgtcttt
    30421 gaggcttatt ttgcgccctg tgacgccttc aaaacggaag taaaagaatt ccttccagaa
    30481 gtccgttggc atgcctgtat cgatgaaaat atcgcctatc agcgcataga tgaagtttcg
    30541 ttgctgcaca gtaaatcgac gtggatcaat aaatcgaact tcaataatcc gatcgccatc
    30601 atatccgtca tacatcgtct ttagtcgatc aatgttcact tcttcattga tcgttgcacc
    30661 tatgtctttt cctttgaact ttttcagaac cgctgaatat gaatcgatca atggtttaaa
    30721 cactcatatc acttctcttt tgtttcttct ctgtactgat cttcaatcca gttaacgcct
    30781 cgttttagaa tgcccaagtc tctcttggtc catttactgt catcagcggt tatagaagcc
    30841 gcatcagtca atgcaacaat tgcttcatca attgattttt cgtacttgtt agcaaccagt
    30901 tgtaaagcat ccaagaatag ctttttgctt ctttgagtag ctggttcaag catcgagaca
    30961 tcttctggca tatcttcgcc agcaaatata tatagcccta gcccaaacat tgctagattt
    31021 tttacaagac agcgcatgat cgttttgttg atatcaaaca tagttgctgc ttcaactcgc
    31081 ttttcgattt ttccaacaat ctcttttttc ttcgtttcgt tattccactg ataatcattg
    31141 acttcgtagg tatatggctc atctttcatt gccttgtttg caccatccat gactggtaac
    31201 cacatgtcac gctttactcc gttgactgtg atactggtaa aaaccatata gcctgttttc
    31261 ttatcaaaga catacggcag atatgtttct ggatcatgat agatttcata atctactgcc
    31321 tcacacactt tgctaacttc tgcccaagcc catgcccacg aaagataagt gaggttattt
    31381 cgtttttcag taacatcttt cacatttatt ttgtataagg tattaaaaag cgtattatca
    31441 cttctatttt tcaaaggttg ttccttttca ctcatcaaat tctgcctcca tttcagcaat
    31501 gtatttctta cctgatccgt aataagagat atcaatcaag ttatctctgt catactcttc
    31561 tagcgcatca atcaagccat cttcgatgac atagatatat tcagtttttt tggacttcct
    31621 cgataaatgg ataagataga catgatccca aatactcaca aaatttccca aatcgtcttg
    31681 atcacatgct agttcttcat ccgtcaaaag attacgtctg atttttcgat tgcttgtttc
    31741 cttgatattc gatttgcccc aactaggatc agtcaaatat tgatctagag tggaaagttc
    31801 ttttttcatg tggtaacatc tccttagatg tattttcttt gtgactcatt gctttggtcg
    31861 gctgagtcac ttttttattt gttgccatgc tttttgctta tcaatatgtt gttggcttag
    31921 gatgtttggt ttattgtgtc tccaccagcg attagcaatt attacgccta tttttagcgc
    31981 ttcagctcta ttcatcctct aacaactcca tttgcttaat aattgtcatc gtcgctgtag
    32041 aaggcatcca attgttaata aaatcgataa catcatcgaa ctgttttgcc ttgatacgat
    32101 ttctagctac tgctccagtg attcgcttaa ttccgccatt caagtcttta aacaattctg
    32161 cttttgcttt gctatttaaa tgctgctgat tacaaataac tctaattttg ttgcgaacca
    32221 tattagaaat tgttcggtaa tctggttcag ctagaagttg attgttcttc aaatcgttta
    32281 aatcagactc gatagcgtct actctttcat tcgtttcttc gtttgcagct agagcaagta
    32341 atgccatttc tcgtttagtt gctggaattg atggcaattg gtcttttaat tctttttcca
    32401 tcttgttgaa ggcctcaata tattgaagtt taaaacttaa agctttctta ccagtgaagc
    32461 ccattgctaa tagagagaag ccgtctctat tcatataaat aattcggtat gattgtttat
    32521 tttgaggatg aacataagta tcttcccaaa ataggtctgc ccaattttcg gcaaccccct
    32581 cttttagttc atcaatagcc gtcaaaacat gtctgtgctt tttatcgaag ctttctgcaa
    32641 cttgtaagct cgtagtcaca gcttctttat ttttcaaaat tactaattct tgcattattt
    32701 cttctctcct ttttgatata attctggtgg ggtgaaaaca tgagcagaaa aattgataat
    32761 ataatattgt taacaactat tttttattta gtaatcggag caactctttc cttactgtca
    32821 tttttgtcca acagtagttt gattgtgtta aatcaaaaaa gcatattaac agaatttgcc
    32881 cacatagatt acttctcttt ttttaaaact ggactaatat gtttatttct atcttcattt
    32941 atcctctata taggaactac attttgtcta atggtgctta atttaaaaat gtttataatt
    33001 gaaaaatgga tagttattat cgcaaagatt ttctcatcga tatcaattat cattccaatg
    33061 acattgacgt tttctaaaga gcaatttaac gtagctacta cattcgtatc ttttatagct
    33121 ctattttcgt ttgctgttcc taaaactttt caaagcaaat ctagcaacgc aaaggttaat
    33181 gtagaaaaca ctgatagaaa aaatgatgaa agttattaaa aaaaataatt ctttcattta
    33241 ttgtcaaacc tttcttatag acattagact tttttagtca gtccctcccg actggctttt
    33301 tcgctctgta ctcagcttca tcaagcccaa tgaaaatcca aagcatgtaa acgatcgtgc
    33361 cgattaatgc ttgtctacta ccccatagac ctaaagcgta gataattagt ggcgcgctga
    33421 atactaatgt tcgattaaac tttcccatgt tgacctcctt taaattttgt atttagccat
    33481 cagttcatcg atgtcctttt tgtcatatct taaaaagcca tctatacgaa tttcttttaa
    33541 accgtgagct attaacttct caaaaccttg atcgtttaca cctcccacat attttctagc
    33601 ttcgatcttt tttaaatatc gtgtttctga caaattcttt tttgaaagtt gactaagtgc
    33661 gtcctttaca acttcaacaa ttgcaattct taaacgagtc aaaaactctt cgcttagaat
    33721 attcatgact tgcctcctat cgaattctgt aatcacggat gatctctaga atagttctgt
    33781 ttgcctttgg tccactccag tgtccatcaa gaatttgttg cattcgtaca cgcgtgtgac
    33841 cataagcagt agccaaatca tccatagtta ttccattctc tttcataaac tctttgatcg
    33901 ccgcacgacc gttatccaag tttgacatta acccacttcc tttttttata aatttgtaag
    33961 ttaaaatgat agaaaaaata taatttccat tgacattttt acactataga gtaaaatgaa
    34021 tacatagaga aataagccta taacaaaacc ttttatttgc acttggtcgc caaactttat
    34081 gctataaggt gtgtttttag tttgcttttt tctatcaaat taacttac
//
LOCUS       DACCZY010000014        33820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCZY010000014 DACCZY010000000
VERSION     DACCZY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.014"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            53..1951
                     /locus_tag="IVZ67_000554"
     CDS             53..1951
                     /locus_tag="IVZ67_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8164655.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2281..3000)
                     /locus_tag="IVZ67_000555"
     CDS             complement(2281..3000)
                     /locus_tag="IVZ67_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8164656.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3337..3669
                     /locus_tag="IVZ67_000556"
     CDS             3337..3669
                     /locus_tag="IVZ67_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164657.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3656..5659
                     /locus_tag="IVZ67_000557"
     CDS             3656..5659
                     /locus_tag="IVZ67_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8164658.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5678..6148
                     /locus_tag="IVZ67_000558"
     CDS             5678..6148
                     /locus_tag="IVZ67_000558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8164659.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6169..6753
                     /locus_tag="IVZ67_000559"
     CDS             6169..6753
                     /locus_tag="IVZ67_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8164660.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6768..7754
                     /locus_tag="IVZ67_000560"
     CDS             6768..7754
                     /locus_tag="IVZ67_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8164661.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7744..8055
                     /locus_tag="IVZ67_000561"
     CDS             7744..8055
                     /locus_tag="IVZ67_000561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8164662.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8077..9858
                     /locus_tag="IVZ67_000562"
     CDS             8077..9858
                     /locus_tag="IVZ67_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8164663.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9851..11227
                     /locus_tag="IVZ67_000563"
     CDS             9851..11227
                     /locus_tag="IVZ67_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8164664.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11234..11869
                     /locus_tag="IVZ67_000564"
     CDS             11234..11869
                     /locus_tag="IVZ67_000564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8164665.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11899..13254
                     /locus_tag="IVZ67_000565"
     CDS             11899..13254
                     /locus_tag="IVZ67_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8164666.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13446..14840)
                     /locus_tag="IVZ67_000566"
     CDS             complement(13446..14840)
                     /locus_tag="IVZ67_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8164667.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14932..15903
                     /locus_tag="IVZ67_000567"
     CDS             14932..15903
                     /locus_tag="IVZ67_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8164668.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15900..16682
                     /locus_tag="IVZ67_000568"
     CDS             15900..16682
                     /locus_tag="IVZ67_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8164669.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16851..17222)
                     /locus_tag="IVZ67_000569"
     CDS             complement(16851..17222)
                     /locus_tag="IVZ67_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8164670.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17356..17799
                     /locus_tag="IVZ67_000570"
     CDS             17356..17799
                     /locus_tag="IVZ67_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8164671.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="IVZ67_000571"
     CDS             complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="IVZ67_000571"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8164672.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18670..20037)
                     /locus_tag="IVZ67_000572"
     CDS             complement(18670..20037)
                     /locus_tag="IVZ67_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8164673.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20021..20851)
                     /locus_tag="IVZ67_000573"
     CDS             complement(20021..20851)
                     /locus_tag="IVZ67_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8164674.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21014..21451
                     /locus_tag="IVZ67_000574"
     CDS             21014..21451
                     /locus_tag="IVZ67_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8164675.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21527..22147
                     /locus_tag="IVZ67_000575"
     CDS             21527..22147
                     /locus_tag="IVZ67_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8164676.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22336..22842
                     /locus_tag="IVZ67_000576"
     CDS             22336..22842
                     /locus_tag="IVZ67_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8164677.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22859..23758
                     /locus_tag="IVZ67_000577"
     CDS             22859..23758
                     /locus_tag="IVZ67_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8164678.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23845..24729
                     /locus_tag="IVZ67_000578"
     CDS             23845..24729
                     /locus_tag="IVZ67_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8164679.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24921..24992)
                     /locus_tag="IVZ67_000579"
     tRNA            complement(24921..24992)
                     /locus_tag="IVZ67_000579"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24957..24959),aa:Arg,seq:ccg)
     gene            25052..26386
                     /gene="rpoN"
                     /locus_tag="IVZ67_000580"
     CDS             25052..26386
                     /gene="rpoN"
                     /locus_tag="IVZ67_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8164680.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26589..27626
                     /locus_tag="IVZ67_000581"
     CDS             26589..27626
                     /locus_tag="IVZ67_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8164681.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            27667..28668
                     /gene="gap"
                     /locus_tag="IVZ67_000582"
     CDS             27667..28668
                     /gene="gap"
                     /locus_tag="IVZ67_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8164682.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28778..29968
                     /locus_tag="IVZ67_000583"
     CDS             28778..29968
                     /locus_tag="IVZ67_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8164683.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30072..30827
                     /locus_tag="IVZ67_000584"
     CDS             30072..30827
                     /locus_tag="IVZ67_000584"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8164684.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30943..32241
                     /gene="eno"
                     /locus_tag="IVZ67_000585"
     CDS             30943..32241
                     /gene="eno"
                     /locus_tag="IVZ67_000585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8164685.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32640..33779
                     /locus_tag="IVZ67_000586"
     CDS             32640..33779
                     /locus_tag="IVZ67_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8164686.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11177 a         5973 c         7207 g         9463 t
ORIGIN      
        1 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
       61 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
      121 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
      181 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
      241 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
      301 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
      361 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
      421 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
      481 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
      541 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
      601 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
      661 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
      721 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
      781 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
      841 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
      901 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
      961 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
     1021 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
     1081 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
     1141 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
     1201 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
     1261 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
     1321 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
     1381 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
     1441 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
     1501 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
     1561 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
     1621 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
     1681 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
     1741 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
     1801 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
     1861 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
     1921 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
     1981 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
     2041 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
     2101 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
     2161 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
     2221 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
     2281 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
     2341 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
     2401 ttcaatgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
     2461 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
     2521 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
     2581 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
     2641 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
     2701 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
     2761 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
     2821 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
     2881 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
     2941 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
     3001 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
     3061 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
     3121 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
     3181 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
     3241 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
     3301 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
     3361 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
     3421 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
     3481 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
     3541 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
     3601 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
     3661 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
     3721 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
     3781 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
     3841 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
     3901 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
     3961 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
     4021 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
     4081 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
     4141 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
     4201 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
     4261 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
     4321 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
     4381 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
     4441 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
     4501 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
     4561 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
     4621 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
     4681 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
     4741 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
     4801 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
     4861 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
     4921 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
     4981 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
     5041 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
     5101 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
     5161 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
     5221 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
     5281 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
     5341 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
     5401 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
     5461 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
     5521 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
     5581 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
     5641 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
     5701 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
     5761 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
     5821 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggtacgc aaggtttgta
     5881 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
     5941 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
     6001 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
     6061 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
     6121 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
     6181 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
     6241 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
     6301 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
     6361 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
     6421 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
     6481 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
     6541 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
     6601 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
     6661 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
     6721 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
     6781 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
     6841 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
     6901 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
     6961 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
     7021 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
     7081 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
     7141 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
     7201 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
     7261 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
     7321 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
     7381 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
     7441 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
     7501 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
     7561 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
     7621 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
     7681 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
     7741 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
     7801 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
     7861 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
     7921 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
     7981 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
     8041 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
     8101 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
     8161 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
     8221 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
     8281 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
     8341 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
     8401 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
     8461 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
     8521 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
     8581 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
     8641 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
     8701 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
     8761 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
     8821 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
     8881 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
     8941 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
     9001 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
     9061 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
     9121 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
     9181 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
     9241 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
     9301 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
     9361 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
     9421 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
     9481 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
     9541 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
     9601 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
     9661 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
     9721 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
     9781 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
     9841 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
     9901 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
     9961 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    10021 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    10081 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    10141 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    10201 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    10261 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    10321 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    10381 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    10441 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    10501 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    10561 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    10621 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    10681 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    10741 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    10801 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    10861 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    10921 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    10981 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    11041 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    11101 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    11161 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    11221 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    11281 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    11341 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    11401 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    11461 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    11521 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    11581 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    11641 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    11701 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    11761 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    11821 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    11881 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    11941 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    12001 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    12061 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    12121 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    12181 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    12241 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    12301 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    12361 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    12421 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    12481 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    12541 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    12601 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    12661 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    12721 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    12781 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    12841 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    12901 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    12961 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    13021 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    13081 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    13141 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    13201 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    13261 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    13321 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    13381 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    13441 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    13501 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgtacgtgta
    13561 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    13621 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    13681 ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta
    13741 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    13801 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    13861 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    13921 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    13981 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    14041 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    14101 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    14161 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    14221 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    14281 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    14341 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    14401 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    14461 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    14521 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    14581 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    14641 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    14701 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    14761 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    14821 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    14881 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    14941 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    15001 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    15061 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    15121 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    15181 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    15241 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    15301 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    15361 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    15421 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    15481 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    15541 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    15601 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    15661 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    15721 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    15781 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    15841 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    15901 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    15961 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    16021 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    16081 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    16141 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    16201 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    16261 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    16321 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    16381 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    16441 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    16501 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    16561 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    16621 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    16681 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    16741 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    16801 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    16861 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    16921 ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt
    16981 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    17041 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    17101 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    17161 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    17221 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    17281 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    17341 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    17401 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    17461 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    17521 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    17581 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    17641 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    17701 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    17761 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    17821 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    17881 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    17941 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    18001 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    18061 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    18121 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    18181 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    18241 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    18301 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccatcac
    18361 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    18421 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    18481 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    18541 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    18601 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    18661 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    18721 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    18781 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    18841 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    18901 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    18961 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    19021 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    19081 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    19141 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    19201 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    19261 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    19321 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    19381 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    19441 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    19501 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    19561 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    19621 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    19681 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    19741 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    19801 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    19861 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    19921 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    19981 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    20041 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    20101 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    20161 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    20221 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    20281 cttcttttaa agcagattga taaaatcgct tcatgacttg cccgcgtttt tcctgattac
    20341 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    20401 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    20461 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    20521 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    20581 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    20641 aacctgcatc tttcagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    20701 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    20761 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    20821 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    20881 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    20941 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    21001 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    21061 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    21121 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    21181 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    21241 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    21301 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    21361 cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    21421 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    21481 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    21541 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    21601 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    21661 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    21721 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    21781 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    21841 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    21901 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    21961 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    22021 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    22081 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    22141 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    22201 ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc ccaagtaaat
    22261 acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc
    22321 agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac
    22381 cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg
    22441 aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc
    22501 ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata
    22561 ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt
    22621 gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg
    22681 attttagata aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga
    22741 tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa
    22801 atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat
    22861 gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc
    22921 caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa
    22981 agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat
    23041 tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat
    23101 cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat
    23161 cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg
    23221 attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata
    23281 tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga
    23341 taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga
    23401 aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt
    23461 caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca
    23521 agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat
    23581 agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt
    23641 agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt
    23701 cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac
    23761 gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag
    23821 agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct
    23881 ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa
    23941 ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc
    24001 attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa
    24061 aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg
    24121 gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag
    24181 cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat
    24241 acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat
    24301 cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg
    24361 acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg
    24421 ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg
    24481 tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa
    24541 gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt
    24601 gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg
    24661 gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca
    24721 gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt agttgaacac
    24781 ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc
    24841 accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt
    24901 atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga
    24961 atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag
    25021 ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa
    25081 acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt
    25141 acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt
    25201 ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta
    25261 tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga
    25321 ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct
    25381 ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg
    25441 aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt
    25501 agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg
    25561 agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt
    25621 ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca
    25681 gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag
    25741 cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt
    25801 tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat
    25861 gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga
    25921 atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat
    25981 tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt
    26041 aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg
    26101 gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca ctacacgaat
    26161 tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct
    26221 gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga aattggttga
    26281 attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt
    26341 attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact
    26401 agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca aaactgttag
    26461 acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc
    26521 tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg
    26581 agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag
    26641 tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg attggacgtc
    26701 gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa acagatcttt
    26761 tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg
    26821 aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg
    26881 aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg
    26941 aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac
    27001 tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag
    27061 ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag
    27121 gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta
    27181 aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg gcaacttaca
    27241 attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt
    27301 gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa
    27361 aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca
    27421 atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg
    27481 aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag
    27541 cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg
    27601 agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa
    27661 tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc
    27721 cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct
    27781 aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt
    27841 gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc
    27901 aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt
    27961 ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt
    28021 atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca
    28081 ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct
    28141 atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac
    28201 gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt
    28261 gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt
    28321 ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca
    28381 acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc
    28441 aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt
    28501 tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta
    28561 atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca
    28621 tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg
    28681 tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg
    28741 ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga
    28801 tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga
    28861 cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct
    28921 agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga
    28981 taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga
    29041 agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa
    29101 tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga
    29161 aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa
    29221 cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg
    29281 tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt
    29341 agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat
    29401 cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac
    29461 ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa
    29521 agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga
    29581 caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat
    29641 tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac
    29701 attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa
    29761 ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac
    29821 aatcatcggt ggcggagact cagccgcagc agcagaacaa ttaggatttg ctgacaaatt
    29881 cacacacatc tcaacaggtg gcggcgcaag cttggaattg cttgaaggta aagaattacc
    29941 aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat
    30001 cgaacaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag
    30061 tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat
    30121 cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg
    30181 attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta
    30241 cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg
    30301 aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag
    30361 aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagcg aaagctatct
    30421 ttgcaaatgg tatgacacca atcctatgct gtggcgaatc tttagaaacc tatgaagcag
    30481 gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg
    30541 aacaggtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat
    30601 cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac
    30661 tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag
    30721 aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa
    30781 gcttgcaagc agattcattc ttgtcattgt tagacgctgt aaaataattg ttgaaaattt
    30841 gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg
    30901 gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat
    30961 gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac
    31021 acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac
    31081 gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa
    31141 gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac
    31201 caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta
    31261 ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta
    31321 gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg
    31381 atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg
    31441 atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc
    31501 cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt
    31561 ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc
    31621 gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca
    31681 gaattctacg acaaagaaaa aggtgtttac gtactagctg attcaggcga aggcgaaaaa
    31741 acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca
    31801 atcgaagacg gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta
    31861 ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct
    31921 gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca
    31981 ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta
    32041 tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaac
    32101 gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa
    32161 ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc
    32221 tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga
    32281 tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag
    32341 gtagagagtt tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa
    32401 aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg
    32461 atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa ataggaggga
    32521 agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct
    32581 atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga
    32641 tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa tcagtgaatg
    32701 gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat gacataacag
    32761 gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc
    32821 aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct ggtgatgaat
    32881 cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg
    32941 gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag
    33001 agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg
    33061 gtgaactagg tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga
    33121 tcatcggaga gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata
    33181 cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa
    33241 tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa
    33301 caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga
    33361 atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag
    33421 aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag
    33481 agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc
    33541 cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta cctttagttg
    33601 gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg
    33661 tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg
    33721 ataattattt ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag
    33781 atttttttaa taaatgaaaa aacagagcca gattagcatt
//
LOCUS       DACCZY010000015        33811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCZY010000015 DACCZY010000000
VERSION     DACCZY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.015"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            117..1082
                     /locus_tag="IVZ67_000587"
     CDS             117..1082
                     /locus_tag="IVZ67_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8164687.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1139..1369)
                     /locus_tag="IVZ67_000588"
     CDS             complement(1139..1369)
                     /locus_tag="IVZ67_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8164688.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1455..2342)
                     /locus_tag="IVZ67_000589"
     CDS             complement(1455..2342)
                     /locus_tag="IVZ67_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8164689.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2431..3654)
                     /locus_tag="IVZ67_000590"
     CDS             complement(2431..3654)
                     /locus_tag="IVZ67_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8164690.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3671..4750)
                     /locus_tag="IVZ67_000591"
     CDS             complement(3671..4750)
                     /locus_tag="IVZ67_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8164691.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4881..6608)
                     /gene="ptsP"
                     /locus_tag="IVZ67_000592"
     CDS             complement(4881..6608)
                     /gene="ptsP"
                     /locus_tag="IVZ67_000592"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8164692.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6608..6874)
                     /locus_tag="IVZ67_000593"
     CDS             complement(6608..6874)
                     /locus_tag="IVZ67_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8164693.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7353..9587
                     /locus_tag="IVZ67_000594"
     CDS             7353..9587
                     /locus_tag="IVZ67_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8164694.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9735..10055
                     /locus_tag="IVZ67_000595"
     CDS             9735..10055
                     /locus_tag="IVZ67_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8164695.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10260..10484)
                     /locus_tag="IVZ67_000596"
     CDS             complement(10260..10484)
                     /locus_tag="IVZ67_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8164696.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10682..12262)
                     /locus_tag="IVZ67_000597"
     CDS             complement(10682..12262)
                     /locus_tag="IVZ67_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8164697.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12663..14039)
                     /locus_tag="IVZ67_000598"
     CDS             complement(12663..14039)
                     /locus_tag="IVZ67_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8164698.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14167..15285)
                     /locus_tag="IVZ67_000599"
     CDS             complement(14167..15285)
                     /locus_tag="IVZ67_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8164699.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15427..15861
                     /locus_tag="IVZ67_000600"
     CDS             15427..15861
                     /locus_tag="IVZ67_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164700.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15939..16472)
                     /locus_tag="IVZ67_000601"
     CDS             complement(15939..16472)
                     /locus_tag="IVZ67_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8164701.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16566..17744)
                     /gene="mutY"
                     /locus_tag="IVZ67_000602"
     CDS             complement(16566..17744)
                     /gene="mutY"
                     /locus_tag="IVZ67_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8164702.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17737..18534)
                     /gene="recX"
                     /locus_tag="IVZ67_000603"
     CDS             complement(17737..18534)
                     /gene="recX"
                     /locus_tag="IVZ67_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8164703.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18641..20023
                     /gene="rlmD"
                     /locus_tag="IVZ67_000604"
     CDS             18641..20023
                     /gene="rlmD"
                     /locus_tag="IVZ67_000604"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8164704.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20197..20775)
                     /locus_tag="IVZ67_000605"
     CDS             complement(20197..20775)
                     /locus_tag="IVZ67_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8164705.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21097..21567
                     /locus_tag="IVZ67_000606"
     CDS             21097..21567
                     /locus_tag="IVZ67_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8164706.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21675..22901)
                     /locus_tag="IVZ67_000607"
     CDS             complement(21675..22901)
                     /locus_tag="IVZ67_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8164707.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23655..23855)
                     /locus_tag="IVZ67_000608"
     CDS             complement(23655..23855)
                     /locus_tag="IVZ67_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8164708.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23989..24597)
                     /locus_tag="IVZ67_000609"
     CDS             complement(23989..24597)
                     /locus_tag="IVZ67_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8164709.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24682..25152)
                     /locus_tag="IVZ67_000610"
     CDS             complement(24682..25152)
                     /locus_tag="IVZ67_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8164710.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25200..26114)
                     /locus_tag="IVZ67_000611"
     CDS             complement(25200..26114)
                     /locus_tag="IVZ67_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8164711.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26260..27063
                     /locus_tag="IVZ67_000612"
     CDS             26260..27063
                     /locus_tag="IVZ67_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8164712.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27241..28188
                     /locus_tag="IVZ67_000613"
     CDS             27241..28188
                     /locus_tag="IVZ67_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8164713.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28256..28549)
                     /locus_tag="IVZ67_000614"
     CDS             complement(28256..28549)
                     /locus_tag="IVZ67_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8164714.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28577..29242)
                     /locus_tag="IVZ67_000615"
     CDS             complement(28577..29242)
                     /locus_tag="IVZ67_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8164715.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29382..30014)
                     /locus_tag="IVZ67_000616"
     CDS             complement(29382..30014)
                     /locus_tag="IVZ67_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8164716.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30021..31088)
                     /locus_tag="IVZ67_000617"
     CDS             complement(30021..31088)
                     /locus_tag="IVZ67_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8164717.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31085..31816)
                     /locus_tag="IVZ67_000618"
     CDS             complement(31085..31816)
                     /locus_tag="IVZ67_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8164718.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31985..32464)
                     /gene="greA"
                     /locus_tag="IVZ67_000619"
     CDS             complement(31985..32464)
                     /gene="greA"
                     /locus_tag="IVZ67_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8164719.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32600..33775)
                     /gene="mltG"
                     /locus_tag="IVZ67_000620"
     CDS             complement(32600..33775)
                     /gene="mltG"
                     /locus_tag="IVZ67_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8164720.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        10088 a         6862 c         5604 g        11257 t
ORIGIN      
        1 acatattttg attatcaaag tattttctgt gaacagaact atagaattcc ctaaaacttg
       61 tccattattt caaaatttgt tataggcttt ttattcaaag cgtaaaagga gatcctatgt
      121 taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt ctatcttatg aaagaccgag
      181 atggtaaggt catctatgtt ggtaaagcaa aacggctcaa aaatagagta agcagctatt
      241 ttcatcagaa taaacagcat tcaaaaaaag tattacgtat gattcatcat ataacagatt
      301 ttgatttcgt cgtagtagac acggaattag atgctttatt gctcgagtgt caattgatcc
      361 aacattaccg tcctttttat aaccgacaaa tgaattattt cagtaattat aattatgtac
      421 atattaccaa taagggattt gtattaacag atactcctac ggctcgaaca tacgggcctt
      481 ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat ggaagaaact tatcaaatgc
      541 cttggttgtc tgaaattagt ttgttagctt tgcgagtcca gcttcctgac ctacaggaaa
      601 tgacttttga acagaaaaaa aaggaactcc aagggctgtt tcaaggccga aataaaaagc
      661 tactgactta cttgaaaaaa agacaacaac atttcatcta tcagttgaat ttcgaaaaag
      721 ccggtatgct tcaaaaagat atcgaattag ttacttattt tattagacgg atccaggaac
      781 aaaagcagtt tttaagaaca ccatcgctca ccttcagcat gccattagca gcagatgaga
      841 gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc agaaacaatg attacctctg
      901 gtgacgtttc tcctgacttc tattatgaaa agaaagaagc tcatttatct ttgaaacgtc
      961 aattgtcaaa agaagaaatc gatcctgtac aaatcttgat cagttaccgc aaaaaattag
     1021 aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag caactaaatt
     1081 agttgcttct cggcttcttt taggcatctt caacttcaaa tgtcaattat cctaaaattt
     1141 attggattaa gtcatagatt tccatcgcta cgatatcgat attatcaaat tcgtattgct
     1201 gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc tttatcataa gttactgtac
     1261 acttctctac gccttctttt tcaaaacggc gaacttgcag ttcattatct gctgactcga
     1321 ccatagcctc cagtcgtttt atgattgcga ctaattgtga atgtgtcatc ggaggtcctc
     1381 cttttcttat ttctgttata ttgtatcaaa agcatagcct acttgcattg attttctcta
     1441 aaaaaactcg ttttttatga ttttttttct ggtatttttc cttcaggcca ccaaagtttg
     1501 atcaaagctt gggttccgtc attctcaaaa cctttatctg ccaactcttc atatagtttc
     1561 aatgcttggg tagttgctgg caatacaaga tccatttttt ctgcttcttc taatgcaatc
     1621 tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc ttttaaaata
     1681 cgaggagaat aattgcttaa agaccagtta gctgcacttc cacctgaaag cgtctcaatc
     1741 actttcttta aatcaagtcc gtttttttgt gcatagacaa gcatttccgt cattcctgtc
     1801 atcgtacctg caatcatgat ctgattagcc atttttgtat gctgaccttt cccagcagaa
     1861 ccatgcaatg taaatgtcgt accaaattct ttgaataaag gcagtacttg atcataaacg
     1921 gcttcttctc caccgaccat gatcgtcagt gtaccattct ttgctcctaa gtcaccaccg
     1981 gataccggtg catctaacgc atctgcgcct ttttcttttg ctgtttttgc aattttttct
     2041 gccaggcttg gtgtacttgt agtcaaatca actaaaatat gtcctgagat atctgcagaa
     2101 aaaatccccg actgtccata gtagatttct tctacatctt tggggtaacc taccattgta
     2161 aagagaatat tactttgttc tgctaccgct tttggtgtat ctgcccagat agctccttct
     2221 gttactaaag gatcagcttt tgcttttgtc cgattataga cagctacttc gtgtcctgct
     2281 tctagtagat ggcgtgcgac agcacttccc atgacgcctg ttccgataaa accaagtttc
     2341 attttttcga cctcctaaaa atcaaaaatc cttgcttcta tgctagtata cgctagaaac
     2401 aaggattttt tcataaaaaa tgtttgaatt ttatctttta aatgaattta attttatttt
     2461 gattcgttca atcgagtctt cttcttcttt tcttcgcatc tcttcttcat gataaacaac
     2521 catgtcttga taaaattcga tcatagcttc gccgaaatgt gtagatgaaa tatcataaag
     2581 tttagcttct aaaacttttg gatctttagg aacttcactg gaaatataat caataagtgc
     2641 tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat cgaacaatcg
     2701 gttcaaataa gcatttcctt ccacgacaca aggtgttccg gctgccatcg cttctgtata
     2761 agtaagccct tgtgtttctg aagtagaagc actgacaaaa taatctgccg ctttataata
     2821 aagtgctaca tcattgttgt ccacttctcc tgtgaactgc acaaattctt ctaactgcag
     2881 ttctgagatc aattcctcta actcttctac ataaggtcct tttcctacga taactaatct
     2941 ggcgttagga taagcggaga tgacctgagg aaacccatta atcaaggctt ggatattttt
     3001 ctcataagaa atcctgctta aagaaagaat catcaaatgg ttttcctgaa gacctagtga
     3061 ctcccttaat ttgctgatat cctgctgagt gatatcagaa cgtttgaatt tttcaatgtc
     3121 gataccagta ggaatcacgc gaagcggtgc tattacacca tagctgcgta atgtatcaat
     3181 aacacgttca cttggacaaa ctacacctgt cgtatgattt gcaaataatc tagacaagta
     3241 ttttacatgg gattgcctga tcacttttcc tttaccgata taatgaaggt agtcttcata
     3301 catcgtatgg taagtatgga tcagaggtat tttcaacttc ttagctacca tcttcccaag
     3361 tatccctgct ccgaattctg tatgggtatg aataagatct agttctagtt ctttggcaac
     3421 caaataagca taccacatgc ctctaacaac aactcttcga tccttgaaag aaataaatgg
     3481 tacacttggc attcgaatca catctttttc caacttatcc gcattaggat cggtcgttgt
     3541 aaaaatatag acttcatgcc catgtctttc taattcttgt tttaatgttt tgatcgatgt
     3601 agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt tcatgttttt
     3661 cgcctccaaa ctagtccttg acctgattta ctttttttaa tttgctagag actttttcat
     3721 tcagtacact ttcgtataca tttttaagtt cttcaccgat tttattgata cttcgctctt
     3781 ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc ttgattggta atctgttgga
     3841 tcagtcggat aaattcttcg tttgtcttcc ccatatagca gttcttgcta tcttctaacc
     3901 agccattata cactggaata tctcgaacaa gtacgttttg tttgcttgct aaagcttcta
     3961 aaacaacgat cccttctgtc tcttcataag aagggaaaaa gaatagatct gcacctgaat
     4021 acgcaccttc tatgatctct ccacgaatgt aaccaggaaa caggacattt tcaggatgat
     4081 cttcttttac aattttccgg atgttccgag gaatcgagta catgggaaca tgaccaaacc
     4141 aaataaatgt gtattcgggc atcttcttag caatttctac gaaatcaatg attcctttac
     4201 gttcgaaaaa caaaccgaca caaatgatta ctttttgatc cggagacagt ttgaaatatt
     4261 ctctgaactt ttgttctttc attggatcag ggtgatattt ctctaagtcg atcccattgg
     4321 aaatagcttg aatcggcaca cggataccat agctttccaa caactgtttt gaataaggtg
     4381 ttggcgtgat cagatagtcc gctttttggt acaatcctac cagatatttt tttacgatag
     4441 gagaaagctg gttcgatctg ataaaggaat ttcggaaatc ttcctctgta gaatgcgcat
     4501 gatagacaac tttttttcct tctcttctgg ctttattgac catattatga ctattgattc
     4561 cgtatgtatt gatgtgcagg atgtcataat cttctttttc atctagtgta taagagatac
     4621 cgacttccgt cagagcacgt ttttgatgat caagcgcacg cccgattcca gattttgcaa
     4681 gcatcttttc actttcaaaa tataataata ccttcacctt acgtccccct tcacttatat
     4741 agtataacat agcctacaaa ctgtcactca aacttaagcc ctatttttgt ctattcttac
     4801 tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc gtagctcttt tacaagccac
     4861 accagctttt gatacgttgt ttattttaca tattcatgaa caagttcaac gacttcttcc
     4921 atcatatcgc attctttcaa tgcacgatca gcaagttcag ccattttcgc tgtgtccaag
     4981 cgtttcatta aactacgcgt tttaagaata gatgtcgcac tcattgagaa ctcatctaga
     5041 cccataccta ctagcaatgg aacagctgtt tgatctccag ccatttctcc acacatacct
     5101 gcccattttc cttcagcgtg tgctgcatca attacatttt tgatcaaacg taaaattgat
     5161 gggttatatg gttggtacaa gtaagaaacg cgttcgttca tacggtctgc tgccattgtg
     5221 tattgaatca aatcgttcgt acctacagag aagaaatcta cttcttttgc aaacttgtct
     5281 gctaagactg ctgctgctgg aatctcgatc atgatgccga cttggatcgt atcagatact
     5341 tccttgcctt cacttatcaa tttttgtttt tcttcttcaa agatcgcttt tgctgcgcgg
     5401 aattctttga gtgtagctac catagggaac atgatacgta aattaccatg aacagatgca
     5461 cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc cttgttcaga taaactgata
     5521 cgcaatgcac gatatcctaa gaatggattc atttcatgag gtaattgcag atatggtagt
     5581 tctttatctc caccgatgtc cattgtacga acaactacag gttttccttc cataccttct
     5641 aatacagctt tgtaagcttc atattggtca tcttctgttg ggaagtctgg agaatccata
     5701 tacaggaatt cagtacggta cagaccaact gcttctccac cattattatg gacaccaact
     5761 agatcttttg gtgttccgat gtttgcagct aattcaaaat gcttgccgtc agctgtgaca
     5821 gtttctgcat tttttaattt ttcccattct gctttttgtg ctgcgtaatc tgcacctgct
     5881 ttttcaaatt cagctttttg ttcatctgta ggatcgatga ttacatcgcc ttcgatcccg
     5941 ttgacagcca aaatatcgcc ttctttgact ttagcagtaa tttcttttgt cccaacgatt
     6001 gctggaattt ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc accaatgtct
     6061 gttacaaacg cttttacaaa attgcgatct aattgtgctg tatcacttgg agtcaagtca
     6121 tgtgcaacaa cgaccacttc ttcgttgatc attgatggat ttggtaaagt aacgcctaat
     6181 aagtgcgcta aaatacgttt agcaacgtca cgaatatctg cagcacgctc ttgcatataa
     6241 gcattatctt ccattgcttc gaacataccg atatacatat ccgttacttc ttttagcgct
     6301 gattcagcat ttacgctgtt gtctttaata ttttgcttga tttgacctac catttctgga
     6361 tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc taagctttgc
     6421 gctgcttttt cacgaatttg ctgcaattct tcagtagatt tagctaatgc agcatccaaa
     6481 cgagcttctt cagccgcagt atcttctact gttgccttac tgaatgttaa atccggttga
     6541 actagcaggt aagcttttgc aacggctaca ccgtcactag cggcaatccc ttttagcatt
     6601 tcaaccatta ttcagataag ccttctttct tcattgtttc aacgattgct gccattgcat
     6661 cagcttcgtc agcaccttct gcagtaatag taacatcaga accttgacct acacctaaag
     6721 acataacgcc catgattgat ttcaagttta ctgatttacc tttatactct aagtttacat
     6781 cagagttaaa tttgcttgct gtttgtacta atagtgtagc tggacgagcg tggatcccag
     6841 tttctgctac tacgtgaaat tctttctttt ccatattaat cggtctcctt taagtgagtg
     6901 ttgtttttat gtgagggttt tcttttttag taattcagag aatcctaaca aatgccaccc
     6961 tttcatctgt aaagattaac attttttttc gttagataca acagttttca ttcaatttca
     7021 gataaaagta ttcaaattat tccggattct ttttcatcct tttcttcaca ttttataaca
     7081 aacgtgttta tttccttttt cttgtcgctt tctcttctcc tgacgtttat cattctttcc
     7141 tttgaccctc tttgttttca tatgaaacga attacttgct tcccttttta tttttagtat
     7201 aataagagtc aaaggtcagg aatggtcaat agagatttca ccttttctac ctcataaatc
     7261 tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa cgagatgtaa aatatggatg
     7321 tacccatttt tgtgaaagga cgtgaatgat tcatgctttg tcaaaactgc ggcaagaatg
     7381 aggcaacgat tcacttgtat gccactgtca atggtcagcg gacacaactc gattactgtc
     7441 agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg tctgactaat atggcccaaa
     7501 atgatccatt tggatttggc agtttggacg atcttttccg ttccatgtct cgccaaatgc
     7561 aacagcaagg aaattatgag caaacaccac caacacaatt tggcggaaac aacaacttca
     7621 atggtggtca gccacctcaa ggcaatacag aaggtttatt gggagaatac ggaatcaata
     7681 tcacagaaca agcacgacaa ggtgatatcg atcctgtgat cggacgcgat gacgaaatca
     7741 aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa tcctgtcttg attggagaac
     7801 caggtgttgg taaaacagca gttgttgaag gtttagcaca aaaaatcgta gatggtgatg
     7861 taccgcaaaa attaatggac aaagaagtca ttcgtttaga tgtcgtatct ttagtccaag
     7921 gtactggtat acgaggacaa tttgaagaac gtatgcaaaa attgattgaa gagatccgtc
     7981 aagcagaaaa tgtgatccta ttcatagatg aagttcacga aatcgtcggt gctggttctg
     8041 ctggtgatgg aaatatggat gctggtaata ttttaaaacc agctttagct cgaggagaat
     8101 tgcaaatggt tggtgctact accttaaatg agtaccgcat catcgaaaaa gacgctgctt
     8161 tagaacgtcg tatgcaacct gtacgggtag atgaaccaac tgtcgaagaa acgatcagta
     8221 ttttgaaagg tttgcaaaaa cgttatgaag actatcatca tgtaaaatat actgatgagg
     8281 ccatcgaagc agccgcaact ttatccaatc gttacgtcca agatcgcttc ttgccggata
     8341 aagccatcga tttgctagat gaaacaggtt ctaagaaaaa cttgacgatc caaatcgttg
     8401 atcctaaaac aatcgagaaa aaactgcaag aagcagaaga acaaaaagtt ttagcttctc
     8461 gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca gatcaataaa cttcaaaaaa
     8521 tgaaagaacg tcagctcact gaagaggaaa cacctgtgat taccgaaaaa gatatggaaa
     8581 aaatcgttga acaacggaca ggtattccag tgggtgaatt aaaagaaaaa gaacagacac
     8641 aactgaaaaa cttagcagat gacttgaaag ctcatgtcat cggacaagat aacgctgtag
     8701 atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt aaacaaacag aatcgtccaa
     8761 tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa aacagagctt gcgaaacaac
     8821 ttgcctacga actattcggt tcacaagatt caatgattcg tttcgatatg tctgaatata
     8881 tggaaaaaca tagtgtctct aaattgattg gttctcctcc aggttatgtc ggatacgaag
     8941 aagctggtca attaacagaa aaagtacgga gaaatcctta cagtcttgtc ttgttagatg
     9001 aagtagaaaa agctcatcca gatgttttgc atatgttctt gcaaatctta gatgatggtc
     9061 gattgacaga tgcacaagga agaacggtca gctttaaaga tacgatcatc attatgacaa
     9121 gtaatgccgg aactggtaaa gtagaagcaa acgttggttt tggtgcggca cgcgaaggtg
     9181 ttactcgttc tgtattgaac caattaaaca actactttac accagaattt ctgaaccgtt
     9241 ttgacgggat catcgaattc agtgcattat caaaagaaaa cttgatgaca atcgtcacac
     9301 taatgctaga cgatgtcaac caaatgctgg cagcacagca acttcacatc gaagttccta
     9361 cgaatgtcaa agaaaaatta gttgacttag gctatgatcc atctatgggt gctcgtcctc
     9421 ttcgacgcac gatccaagaa caaatcgaag atggtatcgc tgaattttat cttgatcacc
     9481 cttctatcca tgagttgaaa gcaaaactgg ataaagatgg aaaaatcatc gttacttcta
     9541 aaccagaacg tttggctaaa gaatcagccg aagaaacggc tgaataaatc cagttttata
     9601 gcgtgagacg gtatttcttt gagaaatgcc gtctcattct tttttcagaa aagtcaaaat
     9661 actgttcatt ttataaaaaa gaaattataa taagagaaac aagcaattaa aaatacataa
     9721 aagaggtgag cgccatgaaa ttggtaaatg taacaaacag ccattcccgt cttgtattaa
     9781 accagcttga aaacactgat gcccatctag tcaaggtcta cacagccgga aataccacta
     9841 ttgtctacac tgaagcccct gaacataatg aaattctgct gatcaatgac aaacgtaaaa
     9901 tccagccgaa agaaatcgaa gatgctaaaa attatttctt agataaaatc aaagacgccg
     9961 tttaccacga ggatgaaatc aaagtaatcg aattggcagg ccttgttgaa atctcgattc
    10021 caaaacatga attaggcgct acattagccg tgtaaacaaa ttttgaacat aataattaaa
    10081 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    10141 aaagcagaag caactccttc ggaaataagc tgaaattcac gaaaattgct tttcaaattt
    10201 ttgtgaattc cagcttattt cttggagtta aacgcttctg tctcagcctc tttttctttc
    10261 tagctttttg atttttctct tttcttttgt acttttctct tttcattttc agttctacgc
    10321 gtgtctttat ttttaacttt ttcctcttta tggatgattg atcgtacttt ttgtgcaatt
    10381 tgctcgtttt tgtcccagtc ttttacatct aataatcgac ataatcgtaa catctcttct
    10441 tttttctgtt tttttctttc acgtctttgc cattctgttt ccatgtgtca cactctcctt
    10501 tctttaatca tatgtacgca aaataatttt tatatttttt tgattagaca tttctatgat
    10561 tttaatatag taaaaagagc cgtcacggaa aatccgtcac ggctctaaac aaaatatgag
    10621 aggctgtttt tgtttggcta ttcttattac ttgatgcagg gcagcttgtg tcaagacttc
    10681 tttacatcaa actttttaat tttacattgg gatatttatc cgcaaaccag cgttctgcaa
    10741 attggttctc gaataagaac aacggttggt cgaaacggtc acgagccaag atattgcgac
    10801 ttgaactcat cttctcatcc aaatcttctg gatcgatcca gcgagcgatt ttgtgtccca
    10861 ttggagacat gattacttcg gcgttatact catttaacat acgatattgg aatacttcga
    10921 attgtagctg accaacagct ccaatgatat attcttccgt tagatatgtt ttataaagct
    10981 gaatcgctcc ttcttgaact aattgataga ttcctttgtg gaaggatttt tgtttcatga
    11041 cattttttgc agtcactttc ataaacagct ctggagtaaa tgaagggagt tcttcataag
    11101 caacttttag ttttccttcg aacaatgtat ccccaatctg ataattgcct gtatcatata
    11161 caccgatgat atcgcctgct actgcttttt cgacgttttc tctcgaatct gccataaatt
    11221 gtgtgacatt gcttaatttc agttttttat ttgtacgttc caaccagaca tccattcctc
    11281 gttcgaatgt tccagaacag attcggacaa aagcgattcg gtcacgatgg gctggattca
    11341 tatttgcttg gatcttgaat acgaaacctg aaaattcttc ctcataagga cttaccatct
    11401 gatcatcttg tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa gtctctaaaa
    11461 aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc tgatcgccac
    11521 gagcaatttt ttcttgatta aatgcatctc cagcctcttt gattagttct acttcttcta
    11581 agacttgttc atataaggaa ttttcagtta gaggatgatt ttcgggaagc agtccatctt
    11641 catccaattc tactaatcgt tcatcctgat aattttccgg acgataaaac tctacacgat
    11701 tatggtagat atcataaaga ccttctagcc ctttacccat accgattggc caattcatcg
    11761 gataagattc gatatctaat agctcttcta attcttctaa caattccaat ggttcacgac
    11821 catcacgatc tagtttgttg ataaaagtaa atatcgggat gccacgtttt tttacaactt
    11881 ggaataattt ctttgtctgt gcttcgatcc cttttgcact atcgattacc atgaccgcac
    11941 tatccacggc catcaaagta cgataagtat cttcagaaaa atcttcatgt cctggtgtat
    12001 ccaagatatt tactcgtttc ccttgataat caaactgcat aacagaacta gtaacagaaa
    12061 tcccacgttg tttctcgatt tccatccagt ctgatttggc aaaattacct gtttttttgc
    12121 cttttacggt cccggcttga cggatagccc caccaaacaa tagtaattgt tctgtaatcg
    12181 ttgttttccc agcatccgga tgggaaatga tcgcaaaagt tctgcgattg tccacttgtt
    12241 gctttaattt tggattattc ataacctaac tccatttctt ccattcaatt atttatattt
    12301 tctctaggca cagattgtcc gatctcttaa agaaagacaa tttcatatag ggctgtgaca
    12361 aaagccgtgt agcagtctct ttttccgagt aaacggcgtt ctagagacag cctatcagca
    12421 ataagcccac ccaagccaaa aatacgaaaa gctgtttttg cttggctgct cttattactt
    12481 aatgcaggat agctttttca caacctttgt caactactac aactgaatca gtatagcatg
    12541 gattcagcga atgaaaaagc caccaaacca taagagtcaa atattttttt gtcagctgac
    12601 taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg tctttcataa ccaagtttcc
    12661 aattatcttc ttggctcgtc atcttcgaat tcttttctga agtatcttcg ttcttcttca
    12721 ggttccgctt ctgtttcttc cggatcatga aaaatgacac ggattttaag gattcgagaa
    12781 ccttccattt cttcagaaac cagcgtaata ttatcgacat caaacgataa cttttcgcct
    12841 tcatccggaa tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc tacatcactc
    12901 atatgcagat cactgtcaaa agcttcattg aactcatcaa tcagcatacg tccttggatg
    12961 atatattcgt gttcatcgat tttttcataa agattctcaa cttcatctga ttcatcatcg
    13021 atttctccga cgatttcttc taaaaggtct tctagtgttg ctaacccaac gacgccgcca
    13081 tattcatcta ataaaatagc catctgattc tgcgtctttt tcatttcata taacagatca
    13141 tcaataaaga ccgtttcagg aacgaataaa ggctcctgca tgattttctt gatatctaaa
    13201 ttatcaaagc cgaatttatg ggcagctttt agtaaattct ttgtatgaag gattccgact
    13261 actttatctt tgtcttcgtt ataaacaggg attcttgaat agttttcgga taaaacttca
    13321 ttcacatttt cttccacagt gtcgttgata tcaatcataa atgcatccgt tcgtgggacc
    13381 atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat ttctaattct
    13441 tcattattca atacgccttc gttttcaagc atataacgca tttcatctct ggtcattttt
    13501 gaatcttcat catcaaaagt catcggcgtg atacgcgcca ataaacttgt ggaagcagaa
    13561 agcagccaga cgaagggctt agctactaca ccaatcaagc gtatcagccc tgaagtgaac
    13621 tttgcgactt cttctgattt atttaaagca atacgtttag ggtacaattc tccaaaaaca
    13681 atcgatacat aggtcaaaat agccagcaca atgatatttg caatattttt tgccgctgtg
    13741 ccgccaccta ataccggagc taaccgttcc gataaagtat tagctagtga agcaccagac
    13801 aaaatgttga ccaaagtgat tcctacttgg attgtcgata ggaaattatt tggattctgc
    13861 aatattttta aaagcttttg agatttcaca tctccttcat cagctttttg ctcgattcga
    13921 ttcttattga tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt gatcaatgtc
    13981 aaaacgatca ataataaaat ctgcgctaac agcgactgac tctcagggtc agcattcata
    14041 atcgcattct ctccttataa aattaatttt tagtttacga tagaaaaaaa cgtggcacta
    14101 tagccacgtt aaaatggtat cacaaagcaa ttttctttgc aataaaaaca agtgtactaa
    14161 aactacctac gaggtcgtag caattttctt cttgttttca ttttgcttgt cttctttgac
    14221 gattttgatt atataggaaa tgatcgtctt gcagattgcg taaaaaggaa cacctaagaa
    14281 gataccaagc aatccggcaa tatttccagc tactagaaga acgataatga tcgttaatgg
    14341 atggattttc agtgatttcc caattacatt aggataaatg atattcccat ccaactgttg
    14401 gacgattagc acaaccacac aacaaagtaa tgcttttacc ggatcattga agactgttac
    14461 tagaaatgca ggtgctagtc ctagataagg tcctaaatat ggaattaggt ttgtcaatcc
    14521 cgcaatgacc ccaaataaga aagcataatc cacaccaatt accatatatc ctaaaaatgt
    14581 gaaggtacca acgaataaac attcgattgc ttgtccgcta atgtaattag ccaatgtttt
    14641 attcagttgc cccaataact ccacaatttg gtttcttctt ttttctggga aaaactgttg
    14701 gatgtttggc actagttttt ctccgtcttt caacatatag aacaagatga atggtgctgt
    14761 aatgattact acggttgccg aagcgatcgt tccaacgatc gaacctaaac tattggatac
    14821 tccactaagg aattgctgaa tgattgtccc gtaagaaatg tctaattgct caaaatattt
    14881 attgatatct acttccttga acaaaggcaa ttcggctacc tcttttaacc agctttctac
    14941 ctgtttgatg aaatcaggca tattagatgc caatgaggaa atttgctgta ctagacttgg
    15001 gatgacacta agtaaaatcc aaataattgc tgcaactaaa aggagcagca caattgcgac
    15061 agcccatatt cttttgattt ttgctttcat caacagcttg acaataggat tcaataaata
    15121 gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg tcccgatcgg
    15181 ttcgaaaata aagtttattt ctgatgaaat catgatcaat gcagctatga tcaataattc
    15241 aagcgaccaa aacattagtt tagactgtct aagtttttca aacaatagat ttcctccttt
    15301 ctttcttact agtatatatt atattcgtta aaaattcttt tattcttttt ttatttttga
    15361 aaacttgcca tactttttat ttgttttatt ttccagtata ctatacgaaa ggaaggtggc
    15421 tagaccatga aagttgtaca aacaaaagat acaatgagca atatttactt agatgctgta
    15481 cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg agaaatagac
    15541 aaagatgaag cccattgtat ccacttcgtc ctgtactcag ataaaaaaga accgcaagga
    15601 actgtacgtc ttttgccttt agaaaacgga aaaatgaaat tacagcggat ggcgattctt
    15661 tctgaataca gacaccaagg gttaggaaaa atattgattg aagaagcaga aaactttgct
    15721 aagaatcaag gctataatac gattttatta ggggcacagt ccacagctga aactttttac
    15781 gaaaaactag gatatacagc ttacggcgat ccttttgaag atgctggcat gcctcatatt
    15841 tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg
    15901 agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg
    15961 taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc
    16021 cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt cgggtaattc
    16081 atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga
    16141 aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa
    16201 ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg
    16261 aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca
    16321 tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt
    16381 aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc
    16441 gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt
    16501 gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc
    16561 tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt
    16621 tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg
    16681 ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc
    16741 catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag
    16801 agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga
    16861 tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact
    16921 tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa
    16981 taatacgctc cagaatgatt ttgaagagct gcactgatat aataaacgtc ttttggcttt
    17041 gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag
    17101 gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga
    17161 tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct
    17221 tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg
    17281 ctgactactc gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa tgcgatactt
    17341 gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt
    17401 tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg
    17461 gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt
    17521 gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg
    17581 tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta
    17641 tagcgccaag gcaggtttct tttttcttgt tcataccatt gaatgaactg atcttgaaat
    17701 tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc
    17761 cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac
    17821 ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg
    17881 tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat cagctatcgc
    17941 ttgttggatc acttcttgag aaaagccttt cgtcatcaat gtttgttgga tttttctcaa
    18001 taattctttt tggctttttc cgtgattttt tccaaacgct ttttcggctg tctgatgtgc
    18061 attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt
    18121 caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat
    18181 attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa
    18241 gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg
    18301 gatttctttt tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa
    18361 atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt
    18421 taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa
    18481 atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat
    18541 cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat
    18601 gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa
    18661 aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg
    18721 ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt
    18781 gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac
    18841 aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt
    18901 acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt
    18961 ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga
    19021 tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca
    19081 aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg
    19141 cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa
    19201 atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt
    19261 tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt
    19321 cccgcaaaaa aacgcaatga tccgtgagat caatgaacga ctgcccgaag ttgtttcaat
    19381 catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt
    19441 atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg
    19501 tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc
    19561 attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg
    19621 cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat
    19681 cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg
    19741 gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat
    19801 cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc
    19861 tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa
    19921 tttaagtaaa aagttcaaga ttgaatattt acagtccgtc gacatgtttc ctcagactgc
    19981 acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa
    20041 aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta
    20101 tgaatactgt acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg
    20161 ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac
    20221 ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc
    20281 ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt
    20341 gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca
    20401 agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca
    20461 agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc
    20521 atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt
    20581 tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt
    20641 tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc
    20701 aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt
    20761 actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa acgctttatt
    20821 gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag gtgaaaaaag
    20881 taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac atgagaatca
    20941 aatcatatga tctttttcaa aaacatcatt catataaaca aatgttcaac tttttaataa
    21001 caacaattat caattttttt cttacttcac aaactaaata caggtcttgc tgtgagcctt
    21061 catggtaaac taatattata gaaaggagtt agtaaaatga agatgataaa agatattcgt
    21121 ttttgggttt gtgttatagg aattgtcata ttaggctttt tatcagggtt gcttagcgga
    21181 aatcccggaa aatattacta ttctttgcaa cttccaccat ttgcaccacc tagctggata
    21241 tttgggccga tgtggacctt actgtatata ttgatgggta tttctcttta tttgttactt
    21301 aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat tcagttcatt
    21361 tttaatttta tatggtctgc tctatttttt aatttaagaa atattttcat tgcagcaatc
    21421 gatattacac ttttagttat tttcttgtca gtgcttatgt atcagttgtg gctgcatcat
    21481 cgcctggcaa tgtggctcat gattccttat tatttatggg tactattcgc aactctattg
    21541 aattattcaa tttatttttt aaactaagcg ctaaaaagag acgtcctgta atttaacaca
    21601 tagaaaaagt gccacctaag ttagattttt tgtctaattt aggtggcact ctttattaac
    21661 caaactattt ttttctagta cccacttgtt gttccatact cattattctg gtcataacca
    21721 taaccatttt gttcctgtcc agcgtaagga tcataacttt gagtatcttg acttggtgta
    21781 tagtactgac ttgtatcttc tgtttgctga taataggtat tcggatcttg gtactgatca
    21841 tatgtgtttt ctgaatagga agaataatcg gtgtaccctt cactattcga tccatcaccg
    21901 tttgtcgaat cgccatcttc cgaatcatca aagaacatca ttgaagcatt ctcatttttc
    21961 aaattaggta atgtatcgct tgtttttata tttaattgtt tcttcagttc attttggatc
    22021 gaaagaagat cattttttcc taaaatttga tagtagatat cattgatcat ttgtccttcg
    22081 ccttgcaact ggtcttgttt gattgtttca aacgccgggg tgtagttcgt cgctacatca
    22141 agcatgtcat cccaatctaa gtccgtcgtc acattgttct caactgcttt taaaattttt
    22201 ctataattgc ttacgccatc taaacttaaa actttattga cgattttcgt cactacttcc
    22261 cgctgacgac gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat tcgagaatag
    22321 gctaaacctt tttctccatt caatgtttgt tcacctactg gtacatggac accatccaaa
    22381 gtaaagtcaa ttttattatt cactttgatg ccaccgaccg cattgatcaa atcttccatg
    22441 ccatccaaat tgatcgttac ataatgatca ataggaatat cgagcagttg ctcaattgaa
    22501 tccatggcca tttcgacacc accaaatgcg taggcatgat ttaatttatc taccgttcca
    22561 taacctacga tattcgtata aatatctcgg tccaaactga tgatcgttga ttttttttgt
    22621 ttcggattaa ctgttactac catcatcgta tcagaacgtc cttgttccgt tcttcctaat
    22681 ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc aatgcttact
    22741 ctctcagaac gtttggagga tattcgatta gagtttttag aaattttgtt tactgtttgg
    22801 ctcgcttctc ccataaatct gattccgtat gctgataatc cggcaatcag caaaacgaat
    22861 acccctaaca aggttaatag aactttttgc catcgcttca taatacacct ctgtttcaca
    22921 ttttctaaaa tattgtctca tataactgtt caatcagcaa attcactttt taatcataag
    22981 aaaactttaa aaaacatttt tgtgtaacga ttattatttt ttaaaatttc tcaaaattct
    23041 taactatcat accataccgt gaaaaagatc gaaagaccga atacacgcag ttcgcaatcg
    23101 ttttatcttt ttttatgtat tttttccaga aaaagcgaac aacaaggtat tttccaaata
    23161 atcgtgcagt aaaatgatcg cacatacctt tcccatcttt ttaacagtct ttgcctaccg
    23221 ttcagattat cattcgattt cacgacaatt tcttcctatg actggtcctt ttgttaaacc
    23281 catcttctat tcgttttatt tccagctcac tgcatggctc ttaaaatgac tcgaactagt
    23341 gattgcatta ccatcatctt ttaatggata gatatgctcc tcttcaaagt aaaacgattt
    23401 tcttttaaag taatagaatt gacatggtaa tgagtatgta tactagtggt ttcacaaatg
    23461 gtcttttatt caacatccaa aaactgaaat ttctttaaaa tccattacta acgaagaaaa
    23521 tgttccttgc ttgataatta gttaggacat gtcaagtgaa caaatgcatt tacttagcca
    23581 aaaaatctaa aaacttcata aatagacaaa aagacacgac tctctaagaa gtcgtgtctt
    23641 tattcaacaa attattataa tttagtcact tcagcagctt gaggtccgcg tgcaccttca
    23701 acaatcgtga attctacttc ttgaccttct tctaggctct tgaagccgtc tccttgaatt
    23761 gctgagaaat gaacaaatac gtcatctccg ccgtctactg taataaaccc aaatcctttt
    23821 tcattgttga accatttaac cgttccgtgt tccataaatg tatatcctcc aaaaaagttc
    23881 ttgcaagtaa ccttgcaaga acattatacc caatttagaa aacgctagca agctttcgct
    23941 cttttatttt ccaaattatt tttaagactg atatgacgga ggaaatggtt attttgctgc
    24001 ttcgaaacgt ttgtttactt catcccaatt aataacattc cagaatgctg caatataatc
    24061 tggtcggaca tttttatatt tcaaataata agcatgttcc caaacgtcca aacctagaat
    24121 tggtgtcttt ccttccatca atggagaatc ttgatttgca gtagaggtaa tagctaattt
    24181 tccattttcc attacaagcc aagcccatcc agaaccaaat cgtccagcgg ctgctttttt
    24241 gaattcttct ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt cttttatttc
    24301 tcctgtaggt tcgccaccag catttggtgc catgatttcc cagaaaaatg aatggttagc
    24361 atgtccgcca ccattgttac gtacagctgt cttgatatct gttggaatag cgtccatatc
    24421 agataataat tcttctattg ttttttcgcc taattctggg tatttctcaa ttgctgaatt
    24481 taaattcgtc acataagtat tgtgatgctt atcatgatgc agatgcatcg tttcttcgtc
    24541 aatataaggt tccaatgcgt catatgcata aggtagatct ggtaatgtgt aagtcataaa
    24601 aaaattcctc ctcaatgttt tcttaataat gtctacatta caaagagtaa caaagagaag
    24661 gaaattcttc aattgttatg cctagtcgtc tttttcactt tgacctactt cttcttcaaa
    24721 aacctcagtt tctttaaaaa taaacaactt gctgaaaata tagttcgcaa ggataacaat
    24781 aatttgagtg atgattttcg ccacatactc attgctgttc ataacattga tcaacaatac
    24841 cattgccccc atgtccaatc cgtaagaaag gatacggtag aagaagaact taccgcattc
    24901 aataagtgca tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt tgttcgttac
    24961 aaaagcaaac aatacagaac aaaaccacga aatagaattg gataaggcca tacttaaatc
    25021 aaaatattgg cgtgcgagag caaaactaac aaaatttaca atcgtagcca aaccaccaaa
    25081 aaagagatag actagtatct cccaatatcc ttttgcttct aaatatcctt ttaactgtcg
    25141 atactgcttc atgagccaac ctaccttatt aaaaattctt atttatcata ctctatattt
    25201 caataacttt catctttatt taaaaaaatt aatttttttt gatcctttgc ctttgtctct
    25261 gcttggatcg tcacatgtgt aataccatat tttaatttta ataaagaaat tatacgagga
    25321 tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata
    25381 attatctttt cgctatactg ccaaatatgg acatgatgaa catcctttac cccatcgatt
    25441 gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg tatcaaaata
    25501 gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt
    25561 gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc
    25621 acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa
    25681 ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata
    25741 actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat
    25801 gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc
    25861 aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt
    25921 ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct
    25981 gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc
    26041 gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact
    26101 attccttcct ccattttatt tatattggat agtctttaga caagaataac atttttttga
    26161 tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg atacttcgct
    26221 agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt
    26281 atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa
    26341 gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt
    26401 tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg cattcctgat
    26461 tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca
    26521 aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact
    26581 gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt
    26641 cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc
    26701 aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct
    26761 ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat
    26821 ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta
    26881 acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact
    26941 ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct
    27001 tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat
    27061 taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag gttgtgacat
    27121 ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg
    27181 cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact
    27241 atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc
    27301 catactatag cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat
    27361 gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc
    27421 ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca
    27481 aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat
    27541 gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt
    27601 gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta
    27661 atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg
    27721 aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct
    27781 ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta
    27841 acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct
    27901 gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta
    27961 aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa
    28021 gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca
    28081 cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc
    28141 acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca
    28201 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
    28261 gttttttaaa gtgataggtt ggctcctcca ggttgggatt ataatcaaca agtaaatcat
    28321 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
    28381 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
    28441 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
    28501 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
    28561 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
    28621 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
    28681 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
    28741 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
    28801 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
    28861 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
    28921 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
    28981 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
    29041 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
    29101 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
    29161 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
    29221 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
    29281 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
    29341 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
    29401 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
    29461 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
    29521 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
    29581 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
    29641 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
    29701 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
    29761 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
    29821 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
    29881 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
    29941 tcttgaatag ataaataaaa agagacgcct aagcgcacca tttcatggtc atctactaat
    30001 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
    30061 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
    30121 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
    30181 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
    30241 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
    30301 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
    30361 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
    30421 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
    30481 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
    30541 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
    30601 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
    30661 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
    30721 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
    30781 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
    30841 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
    30901 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
    30961 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
    31021 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
    31081 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
    31141 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
    31201 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
    31261 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
    31321 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
    31381 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
    31441 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
    31501 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
    31561 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
    31621 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
    31681 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
    31741 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
    31801 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
    31861 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
    31921 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
    31981 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
    32041 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
    32101 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt
    32161 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
    32221 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
    32281 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
    32341 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
    32401 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
    32461 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
    32521 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
    32581 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
    32641 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
    32701 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
    32761 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
    32821 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
    32881 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
    32941 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
    33001 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
    33061 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
    33121 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
    33181 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
    33241 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
    33301 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
    33361 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
    33421 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
    33481 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
    33541 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
    33601 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
    33661 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
    33721 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
    33781 ctcctcctta ctatcaagtt cgtaacttta t
//
LOCUS       DACCZY010000016        32767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCZY010000016 DACCZY010000000
VERSION     DACCZY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.016"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(28..1260)
                     /locus_tag="IVZ67_000621"
     CDS             complement(28..1260)
                     /locus_tag="IVZ67_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8164721.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1515..2084
                     /locus_tag="IVZ67_000622"
     CDS             1515..2084
                     /locus_tag="IVZ67_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8164722.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2262..2702)
                     /locus_tag="IVZ67_000623"
     CDS             complement(2262..2702)
                     /locus_tag="IVZ67_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8164723.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2860..3624
                     /gene="map"
                     /locus_tag="IVZ67_000624"
     CDS             2860..3624
                     /gene="map"
                     /locus_tag="IVZ67_000624"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8164724.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3656..4579
                     /locus_tag="IVZ67_000625"
     CDS             3656..4579
                     /locus_tag="IVZ67_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8164725.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4804..5043)
                     /locus_tag="IVZ67_000626"
     CDS             complement(4804..5043)
                     /locus_tag="IVZ67_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8164726.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5048..5467)
                     /locus_tag="IVZ67_000627"
     CDS             complement(5048..5467)
                     /locus_tag="IVZ67_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8164727.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5694..6932)
                     /locus_tag="IVZ67_000628"
     CDS             complement(5694..6932)
                     /locus_tag="IVZ67_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8164728.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7039..7674)
                     /locus_tag="IVZ67_000629"
     CDS             complement(7039..7674)
                     /locus_tag="IVZ67_000629"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8164729.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7788..7880)
                     /locus_tag="IVZ67_000630"
                     /pseudo
     CDS             complement(7788..7880)
                     /locus_tag="IVZ67_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8016..8651)
                     /locus_tag="IVZ67_000631"
     CDS             complement(8016..8651)
                     /locus_tag="IVZ67_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8164730.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9163..10305
                     /locus_tag="IVZ67_000632"
     CDS             9163..10305
                     /locus_tag="IVZ67_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8164731.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10298..10504
                     /locus_tag="IVZ67_000633"
     CDS             10298..10504
                     /locus_tag="IVZ67_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8164732.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10583..11776
                     /locus_tag="IVZ67_000634"
     CDS             10583..11776
                     /locus_tag="IVZ67_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8164733.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(11783..11941)
                     /locus_tag="IVZ67_000635"
                     /pseudo
     CDS             complement(11783..11941)
                     /locus_tag="IVZ67_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            11981..13120
                     /locus_tag="IVZ67_000636"
     CDS             11981..13120
                     /locus_tag="IVZ67_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8164734.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13113..13913
                     /locus_tag="IVZ67_000637"
     CDS             13113..13913
                     /locus_tag="IVZ67_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8164735.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13913..14740
                     /locus_tag="IVZ67_000638"
     CDS             13913..14740
                     /locus_tag="IVZ67_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8164736.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14718..15452
                     /locus_tag="IVZ67_000639"
     CDS             14718..15452
                     /locus_tag="IVZ67_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8164737.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15552..16418
                     /gene="rfbA"
                     /locus_tag="IVZ67_000640"
     CDS             15552..16418
                     /gene="rfbA"
                     /locus_tag="IVZ67_000640"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8164738.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16432..17004
                     /gene="rfbC"
                     /locus_tag="IVZ67_000641"
     CDS             16432..17004
                     /gene="rfbC"
                     /locus_tag="IVZ67_000641"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8164739.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17026..18054
                     /gene="rfbB"
                     /locus_tag="IVZ67_000642"
     CDS             17026..18054
                     /gene="rfbB"
                     /locus_tag="IVZ67_000642"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8164740.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18152..19003
                     /gene="rfbD"
                     /locus_tag="IVZ67_000643"
     CDS             18152..19003
                     /gene="rfbD"
                     /locus_tag="IVZ67_000643"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8164741.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19037..21070
                     /locus_tag="IVZ67_000644"
     CDS             19037..21070
                     /locus_tag="IVZ67_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164742.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21114..22394
                     /locus_tag="IVZ67_000645"
     CDS             21114..22394
                     /locus_tag="IVZ67_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8164743.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22604..23410
                     /locus_tag="IVZ67_000646"
     CDS             22604..23410
                     /locus_tag="IVZ67_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164744.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23422..24642
                     /locus_tag="IVZ67_000647"
     CDS             23422..24642
                     /locus_tag="IVZ67_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164745.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24632..26218
                     /locus_tag="IVZ67_000648"
     CDS             24632..26218
                     /locus_tag="IVZ67_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8164746.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26257..28395
                     /locus_tag="IVZ67_000649"
     CDS             26257..28395
                     /locus_tag="IVZ67_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8164747.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28763..29746)
                     /locus_tag="IVZ67_000650"
     CDS             complement(28763..29746)
                     /locus_tag="IVZ67_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8164748.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30124..31515
                     /locus_tag="IVZ67_000651"
     CDS             30124..31515
                     /locus_tag="IVZ67_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8164749.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31531..32571
                     /locus_tag="IVZ67_000652"
     CDS             31531..32571
                     /locus_tag="IVZ67_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8164750.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32590..>32767
                     /locus_tag="IVZ67_000653"
     CDS             32590..>32767
                     /locus_tag="IVZ67_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8164751.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRI"
BASE COUNT        10539 a         5692 c         6479 g        10057 t
ORIGIN      
        1 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
       61 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
      121 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
      181 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
      241 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
      301 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
      361 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
      421 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
      481 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
      541 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
      601 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
      661 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
      721 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
      781 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
      841 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
      901 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
      961 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
     1021 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
     1081 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
     1141 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
     1201 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
     1261 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
     1321 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
     1381 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
     1441 atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     1501 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     1561 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     1621 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     1681 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     1741 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     1801 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     1861 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     1921 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     1981 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2041 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2101 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2161 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     2221 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     2281 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     2341 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     2401 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     2461 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     2521 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     2581 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     2641 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     2701 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     2761 aaaagaaaat aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     2821 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     2881 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     2941 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3001 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3061 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3121 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3181 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     3241 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     3301 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     3361 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     3421 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     3481 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     3541 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     3601 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     3661 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     3721 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     3781 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     3841 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     3901 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     3961 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4021 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4081 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4141 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4201 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     4261 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     4321 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     4381 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     4441 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     4501 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
     4561 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttatt
     4621 tgaaatttta tatctaattt tcaattatcc gcataaacac tatacattca agcattttga
     4681 agtatagaaa aacactcatc ttaccacgaa tttaccacga ttgaatgagc cttgatatga
     4741 ccgtaaaact actaagggag atagcacgaa actgtgtatc tcccgatttt tttatacagt
     4801 tgattacttc actccgattt caaatttcaa tattgctttc atcatgtctg tatgaatttc
     4861 tccttttagt tcttcatcaa cctgcataat gattttacca ctttcagagt ggtaaaaagg
     4921 acgcaagcat aatttagaaa tataaccgtc ataaaacttc aaaatctctt taactgccat
     4981 ttcatctccg cttgacgctt tgcaaatcaa ttcaaaagag ggatagccat aatctttact
     5041 catttcttta cttcctttcg ctcaaaagca tttttaactt ccttagtcct ctagttcttc
     5101 tactgcaaat agcagaacgt gatacatggt acagttctga aatttctctg tcgttcattc
     5161 cttgaaaata acgtaataaa atcgcactac gctgtttttc tgacagtcta tgtaaaactg
     5221 taccaagtgt ttcatcagat acataaattg taaagtgcaa gactttaaat gatacacaat
     5281 cataaaaaca ggtatcaatc gttccacgtt caacttgttt cgtttcggct atttcacaaa
     5341 acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt tcgacttttc actttgttct
     5401 taatgacaat cagcattaaa gaattgaact gtaatctgac gatatgctcg aaagaagatg
     5461 tcctcataat ctcaccccct ttctgctaat gctgaaatat ataaacggaa taccgtttat
     5521 accctttaga aatacagaaa aatggtaaga aaaaagccca cacaaaacat atatttgtgc
     5581 gagccttaaa tactgcaata ttactctttc ttttataaca ctatcctttt agcagatttc
     5641 aaattttctt ttatatattg tttttagcct aacatactat ttaacccctt gtattatatc
     5701 tctaactttt gaaaaattct ttttgaatat attgaaatca tgattgaagc aatagaagct
     5761 acaaacataa caacccatgt ttcttgtata aatacatcga ataaaatacc gtaaaccatt
     5821 tgtccaatag gttgaccgca catagcaact gctaccagcg tagccattat ctttcctact
     5881 aattgcggtg gtgtttgtgt ctgaacaaga gtgtaaattt gaacaacaaa catagtagat
     5941 acacccatag ccacaaagca catcactgta actacccagt aggaaactat tgcaggcaat
     6001 tctaaaaata gcgaaattcc cataagtaaa acagaaacag aacaaataag taacaaggta
     6061 taaaggtttt ttagttttaa ctttgaagca acaagagctg ttaaaatacc accacataaa
     6121 ccacctaatg ataatgctga ttgtgaaaaa ccatatagtg tatctggcat ttttaaaact
     6181 tcagaaatta agataggcag accaacaatt aaaacagcac ttaaaaccaa attgaaaatg
     6241 caaaccaaaa agattacgga tataaaaatg ggtttctcct ttttcaaaaa ggtataacta
     6301 tcttttaaat cgtttttagc aactgcaatt acacctactt cacgaggtct tttttggtgt
     6361 ggaatatgga taaaaatttc cataatggct gaaaaagtaa aacatataat actaacaatt
     6421 aaaatgggat tgataccata aaattgaaaa actactccgc ttatagctgg tccgataaag
     6481 tttgctaaag tatttacttg atttattata gcattaccga cggtaagttt atctgatgat
     6541 accaatagag gaatacttgc ttgtaccgct ggttgatatg caccagaaat accataaagt
     6601 aacatcaaag caacaataaa caaaggtatg ataggaattt ttcccaatga caaataaaat
     6661 actaaaatta tgattgctgt tacaaaatct agtatgacca taatgtttct tttatttgtc
     6721 ctgtcagcaa taataccacc taccagcgac aaaataacca taggtaaaaa agagcaggct
     6781 gtagcaatac caaacaaagc tgatgaacca gtttctttta acaaatagta cggtaatgca
     6841 aatcgtagga tagcgtttcc aaataaagaa attatctgtc ctataacaac aagtataaaa
     6901 tcacgattaa aaagtttttt ttctgcttgc ataaaatctc tcctttttat ttaatcaaac
     6961 agaccgtcgg tttatataat ggtttaaata aactgctctt ttatagagca gtctatcgta
     7021 tagggataac aaatacagtt atttacaaaa ctgtataaat gcagaaattg tttcttcgtc
     7081 taataatgtg tctatgccta ttccatttaa aagggcattg aatgtattgc aacgtttttc
     7141 cataccctct ttatccgtca tttcaactaa tgggttgagc catacattac ttaataccat
     7201 gataacttct gcaatttcat gtgaattttc aatctccaaa gacttatcct tgataccttg
     7261 ttctaaaacg ggttcaacaa attcaggtac tacaatttca tacatttgtt ctatttgcat
     7321 agcaagaaaa cgaggattgt ttaatagttg aggagaaact gtcaataaaa cgctttggtt
     7381 agaattgaat accgctgtct taaatatttc tcttaacttt tctaaaccgt ttaaactatc
     7441 gttatctcta attttagcaa atattactgc attttcattt ccaattcttt gaaaaattgc
     7501 ttttaaaata tcttcctttg aatcgaaatg atgataaata gcacctttag aaagttttgt
     7561 ttcattgata atatcttgca atgatgtgtt atcatatcct ttttcacaaa acatttttgt
     7621 agaaacatct aaaatgagtt ttactgtttc ttctggatat ttatttcgtg ccataactta
     7681 cctccaatac aaaccgttgg tctgattaaa gtatatctta gtattttatg cttgtcaata
     7741 taaagacttg caattttata aaaatgcaga atgcaagcct ttttcattta ttctacaatc
     7801 ttccagttgc tttccttatg cggtgtcaat tcatactgtg atacctgcgt tgcctttgcc
     7861 tgatttacca caaatcagat tttatccctt tcttcctctt gattgcccac aacagaaaag
     7921 aaaaagccct gtcaagagct ttgccaccat tggtggtgct ttgcactctt gatcgggctt
     7981 tttgtttgct gtatcttctc gcaagaggaa aaaagttact cgtcctcatc atcaaaattg
     8041 ttattcctta atacttctcg taacagttcc atatcttctt ttgagttcgg gtttatcatt
     8101 ttcacgactt gataaggtgt cttatactta tgtccctcta tgctttcccc aaacatatcc
     8161 atactgtata aatcatcata gcggtctaat aatttttgaa acttcaaatg ctggataaat
     8221 tctttgattg ggtaaaggtc ggacgcttca atgcttttcc cgttgatata gtctgtaata
     8281 tattcccgta acttttctaa ctcatattcc agccattctt cctcgctgtc aaagaagtta
     8341 tcatagtgtc catgtttcag ttctgcattt agacgttctt cggctcttaa aaactgacag
     8401 acttcctttt cagattgatt gacatacttc cattctcccg acaacaattc attgggcgta
     8461 acgtccagca cttccattat cttttccagc gtttcatagg caggatagtt caaccctctt
     8521 tcaatcttgg aaaggctctg catattgata ccgattttgt ccgcaagttc ctgttgtttc
     8581 attcccctaa attttcttat ggtctgtata ttatttccta aatggcttat tttcattgca
     8641 taaccctcca tatctcgtta tatcttcgct attataagtc tatcatgctt aatatgattt
     8701 gtaaaggcaa aaaaagatat tgacaaataa gcatataaga attactatgt tcttgtaatc
     8761 gaataagcac aatagaatga aatataaaaa cattcaaaaa gaaataccta tctgattaaa
     8821 tccgtagata tgaatatcgg ttgaaatgga acttgttaga aaaggcaaaa cagaacttgc
     8881 tgacaaagta tttctgacac ggtggcggaa gcctctcagg cttaccgcca tgacaccttg
     8941 tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc gagccttgtc tgtgcccccg
     9001 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac
     9061 ctgtgagtag taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg
     9121 tttacaggta tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt
     9181 gcacttgaaa tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt
     9241 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc
     9301 aaggaaacac agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt
     9361 tatctgctcg ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc
     9421 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
     9481 tataaggagc attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
     9541 ggcgtatttt tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca
     9601 cagaacaggg actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga
     9661 cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa
     9721 aaatacagaa atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga
     9781 aaattaagcg gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc
     9841 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
     9901 gatattatca accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa
     9961 gaactgttat tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg
    10021 gaatttcctg actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa
    10081 atgaaccctg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
    10141 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg
    10201 attgatgaat gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg
    10261 gacatatcag acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc
    10321 cgtatgggaa aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga
    10381 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
    10441 caaccgcata cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat
    10501 ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc
    10561 aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct
    10621 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
    10681 gggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
    10741 aaaacgggaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
    10801 cggtatcgac agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
    10861 acagaggaca aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt
    10921 aaaagaggac aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga
    10981 atgggcgtta cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg
    11041 ctcgttaaaa gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt
    11101 tgatttcaag ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga
    11161 ggaacaggag caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta
    11221 tgatgatgtg ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac
    11281 agtagccgac attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag
    11341 taaggaacag ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc
    11401 attaagcaaa gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa
    11461 gtcgatagag gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa
    11521 agttgccatt gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca
    11581 caaggacaat cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag
    11641 gctggcaagc aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat
    11701 cagtatcaca atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca
    11761 gcgtctaatc gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata
    11821 agaagataga cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata
    11881 aaataaggct ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa
    11941 tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat
    12001 ggttataaga ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat
    12061 catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc
    12121 aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt
    12181 attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat
    12241 catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg
    12301 gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac
    12361 gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg
    12421 gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg
    12481 gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc
    12541 tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc
    12601 ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt
    12661 tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc
    12721 tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa
    12781 agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag
    12841 tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc
    12901 tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat tgattattgc
    12961 catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca
    13021 gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca
    13081 gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc
    13141 ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt
    13201 caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg
    13261 agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg
    13321 atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc gattctcgct
    13381 atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct
    13441 tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg
    13501 gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg
    13561 tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag
    13621 gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg
    13681 atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag
    13741 acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt
    13801 atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt
    13861 ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc
    13921 agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg
    13981 accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc
    14041 tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca
    14101 aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg
    14161 ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg aactattgcg
    14221 taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg
    14281 acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt
    14341 tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa
    14401 atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg
    14461 gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga
    14521 acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt
    14581 acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg
    14641 acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg
    14701 gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta
    14761 tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc
    14821 tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata
    14881 tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag
    14941 gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg
    15001 cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc
    15061 agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag
    15121 agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga
    15181 taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt
    15241 tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga
    15301 agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg
    15361 agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg
    15421 cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc
    15481 catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag
    15541 agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt
    15601 taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc
    15661 caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag
    15721 atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat
    15781 atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta
    15841 ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt
    15901 ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg
    15961 tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga
    16021 ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg
    16081 acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga
    16141 ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac
    16201 gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta
    16261 tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc
    16321 gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc
    16381 aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta
    16441 acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc
    16501 ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac
    16561 tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc
    16621 caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat
    16681 gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta
    16741 tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact
    16801 ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat
    16861 gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga
    16921 gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat
    16981 ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt
    17041 gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat
    17101 ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg
    17161 gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta
    17221 gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac
    17281 gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca
    17341 cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt
    17401 tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa
    17461 ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa agctggttca
    17521 gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt
    17581 tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgaat
    17641 atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc
    17701 catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa
    17761 acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta
    17821 gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc aggacacgac
    17881 ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa gcctgagttt
    17941 acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg
    18001 tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga
    18061 gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt
    18121 ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag gaaatgggca
    18181 actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg tttcaacaga
    18241 tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca cagaattaaa
    18301 acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag aagaagaagc
    18361 aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac ttgcagcaaa
    18421 agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa
    18481 aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa
    18541 attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc
    18601 ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac
    18661 ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt
    18721 ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc
    18781 gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag atacagatgt
    18841 agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc
    18901 agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt ggcaagaagc
    18961 acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt ttatcaaata
    19021 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    19081 agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    19141 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    19201 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    19261 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    19321 tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    19381 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    19441 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    19501 ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag ctctatcgct
    19561 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    19621 gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt atttctacaa
    19681 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    19741 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    19801 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    19861 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    19921 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    19981 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    20041 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    20101 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    20161 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    20221 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    20281 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    20341 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    20401 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    20461 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    20521 ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    20581 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    20641 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    20701 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    20761 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    20821 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    20881 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    20941 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    21001 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    21061 cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg agagtggaga
    21121 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    21181 tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca actttgttta
    21241 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    21301 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    21361 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    21421 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    21481 tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    21541 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    21601 tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    21661 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    21721 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    21781 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    21841 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    21901 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    21961 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    22021 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    22081 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    22141 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    22201 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    22261 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    22321 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    22381 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    22441 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    22501 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    22561 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    22621 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    22681 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    22741 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    22801 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    22861 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    22921 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccactt
    22981 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    23041 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    23101 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    23161 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    23221 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    23281 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    23341 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    23401 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    23461 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    23521 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    23581 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    23641 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    23701 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    23761 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    23821 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    23881 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    23941 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    24001 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    24061 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    24121 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    24181 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    24241 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    24301 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    24361 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    24421 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    24481 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    24541 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    24601 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    24661 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    24721 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    24781 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    24841 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    24901 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    24961 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    25021 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    25081 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    25141 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    25201 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    25261 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    25321 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    25381 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    25441 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    25501 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    25561 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    25621 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    25681 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    25741 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    25801 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    25861 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    25921 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    25981 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    26041 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    26101 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    26161 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    26221 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    26281 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    26341 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    26401 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    26461 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    26521 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    26581 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    26641 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    26701 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    26761 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    26821 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    26881 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    26941 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    27001 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    27061 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    27121 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    27181 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    27241 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    27301 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    27361 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    27421 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    27481 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    27541 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    27601 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    27661 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    27721 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    27781 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    27841 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    27901 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    27961 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    28021 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    28081 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    28141 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    28201 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    28261 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    28321 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    28381 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    28441 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    28501 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    28561 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    28621 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    28681 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    28741 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    28801 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    28861 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    28921 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    28981 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    29041 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    29101 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    29161 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    29221 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    29281 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    29341 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    29401 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    29461 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    29521 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    29581 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    29641 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    29701 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    29761 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    29821 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    29881 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    29941 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    30001 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    30061 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    30121 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    30181 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    30241 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    30301 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    30361 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    30421 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    30481 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    30541 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    30601 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    30661 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    30721 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    30781 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    30841 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    30901 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    30961 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    31021 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    31081 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    31141 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    31201 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    31261 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    31321 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    31381 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    31441 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    31501 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    31561 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    31621 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    31681 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    31741 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    31801 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    31861 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    31921 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    31981 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    32041 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    32101 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    32161 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    32221 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    32281 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    32341 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    32401 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    32461 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    32521 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    32581 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    32641 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    32701 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    32761 cgtattt
//
LOCUS       DACCZY010000017        32631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCZY010000017 DACCZY010000000
VERSION     DACCZY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.017"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="IVZ67_000654"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="IVZ67_000654"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8164752.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1932..5585)
                     /gene="rpoC"
                     /locus_tag="IVZ67_000655"
     CDS             complement(1932..5585)
                     /gene="rpoC"
                     /locus_tag="IVZ67_000655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8164753.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5664..9281)
                     /gene="rpoB"
                     /locus_tag="IVZ67_000656"
     CDS             complement(5664..9281)
                     /gene="rpoB"
                     /locus_tag="IVZ67_000656"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8164754.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            10009..10977
                     /locus_tag="IVZ67_000657"
     CDS             10009..10977
                     /locus_tag="IVZ67_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8164755.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10995..11900
                     /locus_tag="IVZ67_000658"
     CDS             10995..11900
                     /locus_tag="IVZ67_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164756.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11897..12703
                     /locus_tag="IVZ67_000659"
     CDS             11897..12703
                     /locus_tag="IVZ67_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8164757.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12860..13828
                     /locus_tag="IVZ67_000660"
     CDS             12860..13828
                     /locus_tag="IVZ67_000660"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8164758.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(14145..14669)
                     /locus_tag="IVZ67_000661"
     CDS             complement(14145..14669)
                     /locus_tag="IVZ67_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8164759.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14956..16767
                     /gene="scm"
                     /locus_tag="IVZ67_000662"
     CDS             14956..16767
                     /gene="scm"
                     /locus_tag="IVZ67_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8164760.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(16922..17908)
                     /locus_tag="IVZ67_000663"
     CDS             complement(16922..17908)
                     /locus_tag="IVZ67_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8164761.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(17931..18497)
                     /locus_tag="IVZ67_000664"
     CDS             complement(17931..18497)
                     /locus_tag="IVZ67_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8164762.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(18511..18927)
                     /locus_tag="IVZ67_000665"
     CDS             complement(18511..18927)
                     /locus_tag="IVZ67_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8164763.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(19384..21318)
                     /locus_tag="IVZ67_000666"
     CDS             complement(19384..21318)
                     /locus_tag="IVZ67_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8164764.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            21620..22555
                     /locus_tag="IVZ67_000667"
     CDS             21620..22555
                     /locus_tag="IVZ67_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8164765.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            22645..23748
                     /locus_tag="IVZ67_000668"
     CDS             22645..23748
                     /locus_tag="IVZ67_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8164766.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            23761..24714
                     /gene="menA"
                     /locus_tag="IVZ67_000669"
     CDS             23761..24714
                     /gene="menA"
                     /locus_tag="IVZ67_000669"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8164767.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(25649..26995)
                     /gene="gor"
                     /locus_tag="IVZ67_000670"
     CDS             complement(25649..26995)
                     /gene="gor"
                     /locus_tag="IVZ67_000670"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8164768.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(27129..28370)
                     /locus_tag="IVZ67_000671"
     CDS             complement(27129..28370)
                     /locus_tag="IVZ67_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8164769.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(28388..29311)
                     /locus_tag="IVZ67_000672"
     CDS             complement(28388..29311)
                     /locus_tag="IVZ67_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8164770.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(29532..32024)
                     /locus_tag="IVZ67_000673"
     CDS             complement(29532..32024)
                     /locus_tag="IVZ67_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8164771.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(32031..32498)
                     /locus_tag="IVZ67_000674"
     CDS             complement(32031..32498)
                     /locus_tag="IVZ67_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8164772.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         9323 a         6989 c         6099 g        10220 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt
     1921 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
     1981 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
     2041 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
     2101 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
     2161 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
     2221 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
     2281 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
     2341 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
     2401 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
     2461 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
     2521 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
     2581 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
     2641 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
     2701 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
     2761 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
     2821 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
     2881 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
     2941 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
     3001 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
     3061 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
     3121 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
     3181 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
     3241 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
     3301 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
     3361 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
     3421 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
     3481 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
     3541 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
     3601 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
     3661 cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg
     3721 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
     3781 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
     3841 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
     3901 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
     3961 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
     4021 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
     4081 aagtagtagt tacctagaac catatcttgg gaaggagtaa caactggttt accatcttta
     4141 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
     4201 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
     4261 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
     4321 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
     4381 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
     4441 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
     4501 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
     4561 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
     4621 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
     4681 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
     4741 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
     4801 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
     4861 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
     4921 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
     4981 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
     5041 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
     5101 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
     5161 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
     5221 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
     5281 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
     5341 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
     5401 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
     5461 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
     5521 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
     5581 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
     5641 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
     5701 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
     5761 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
     5821 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
     5881 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
     5941 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
     6001 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
     6061 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
     6121 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
     6181 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
     6241 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
     6301 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
     6361 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
     6421 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
     6481 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
     6541 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
     6601 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
     6661 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
     6721 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
     6781 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
     6841 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
     6901 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
     6961 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
     7021 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
     7081 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
     7141 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
     7201 atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct
     7261 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
     7321 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
     7381 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
     7441 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
     7501 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
     7561 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
     7621 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
     7681 ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag
     7741 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
     7801 cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
     7861 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
     7921 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
     7981 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
     8041 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
     8101 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
     8161 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
     8221 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
     8281 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
     8341 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
     8401 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
     8461 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
     8521 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
     8581 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
     8641 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
     8701 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
     8761 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
     8821 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
     8881 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
     8941 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
     9001 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
     9061 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
     9121 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
     9181 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
     9241 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
     9301 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag
     9361 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
     9421 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
     9481 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
     9541 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
     9601 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
     9661 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
     9721 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
     9781 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
     9841 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
     9901 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
     9961 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
    10021 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
    10081 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
    10141 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
    10201 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
    10261 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
    10321 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
    10381 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
    10441 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
    10501 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
    10561 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
    10621 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
    10681 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
    10741 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
    10801 ggaatcgact acgaaagctt gggtaaacaa acagggatca tggcagcaaa aatcttagac
    10861 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
    10921 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
    10981 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
    11041 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
    11101 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
    11161 atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc
    11221 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
    11281 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
    11341 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
    11401 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
    11461 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
    11521 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
    11581 ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
    11641 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
    11701 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
    11761 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
    11821 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
    11881 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    11941 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    12001 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    12061 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    12121 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    12181 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    12241 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    12301 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    12361 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    12421 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    12481 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    12541 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    12601 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    12661 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    12721 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    12781 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    12841 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    12901 accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat
    12961 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    13021 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    13081 gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgt
    13141 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    13201 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    13261 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    13321 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    13381 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    13441 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    13501 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    13561 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    13621 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    13681 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    13741 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    13801 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    13861 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    13921 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    13981 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    14041 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    14101 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    14161 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    14221 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    14281 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    14341 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    14401 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    14461 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    14521 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    14581 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    14641 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    14701 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    14761 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    14821 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    14881 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    14941 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    15001 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    15061 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    15121 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    15181 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    15241 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    15301 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    15361 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    15421 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    15481 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    15541 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    15601 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    15661 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    15721 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    15781 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    15841 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    15901 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    15961 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
    16021 acttcaagcg acagtacaga tactacctct accacaagtg atagcacgga tacatcagct
    16081 tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggacac atcagcttca
    16141 agtgacagta cagatactac ctctaccaca agtgacagca cggacacgtc agcttcaagc
    16201 gacagtacag atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac
    16261 agtacagata ctacctctac cacaagtgat agcacggaca cgtcagcttc aagcgacagt
    16321 acggatacat ctaccacaac aagtgatagc acggacacgt cagcttcaag tgacagtaca
    16381 gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag tgatagcacg
    16441 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
    16501 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
    16561 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
    16621 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
    16681 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
    16741 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
    16801 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
    16861 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
    16921 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
    16981 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
    17041 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
    17101 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
    17161 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
    17221 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
    17281 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
    17341 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
    17401 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
    17461 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
    17521 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
    17581 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
    17641 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
    17701 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
    17761 acagctttcc gccggcatgg atcgtttctt tgattgcttg ttcgacaggt ttgtttttca
    17821 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
    17881 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
    17941 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
    18001 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
    18061 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
    18121 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
    18181 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
    18241 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
    18301 agtcgtagcc aaagtagtaa gaagctgtct cgttttttca tggtaaaaat ggcaacgatc
    18361 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
    18421 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
    18481 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
    18541 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
    18601 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
    18661 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
    18721 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
    18781 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
    18841 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
    18901 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
    18961 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
    19021 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
    19081 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
    19141 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
    19201 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
    19261 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
    19321 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
    19381 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
    19441 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
    19501 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
    19561 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
    19621 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
    19681 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
    19741 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
    19801 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
    19861 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
    19921 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
    19981 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
    20041 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
    20101 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
    20161 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
    20221 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
    20281 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
    20341 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
    20401 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
    20461 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
    20521 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
    20581 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
    20641 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
    20701 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
    20761 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
    20821 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
    20881 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
    20941 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
    21001 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
    21061 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
    21121 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
    21181 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
    21241 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
    21301 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
    21361 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
    21421 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
    21481 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
    21541 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
    21601 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
    21661 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
    21721 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
    21781 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
    21841 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
    21901 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
    21961 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
    22021 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
    22081 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
    22141 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
    22201 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
    22261 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
    22321 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
    22381 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
    22441 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
    22501 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
    22561 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
    22621 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
    22681 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
    22741 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
    22801 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
    22861 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
    22921 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
    22981 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
    23041 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
    23101 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
    23161 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
    23221 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
    23281 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
    23341 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
    23401 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
    23461 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
    23521 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
    23581 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
    23641 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
    23701 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
    23761 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
    23821 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
    23881 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
    23941 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
    24001 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
    24061 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
    24121 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
    24181 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
    24241 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
    24301 gctttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
    24361 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
    24421 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
    24481 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
    24541 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
    24601 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
    24661 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
    24721 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
    24781 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    24841 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    24901 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
    24961 tttatcagag gttgtgaaaa agtcgcttag tttcgagtat taagaaaaat tttgtgaaaa
    25021 tggctttcca catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    25081 gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta ttagagaaac aaatggaaaa
    25141 atagttcctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacact
    25201 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
    25261 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
    25321 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
    25381 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
    25441 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
    25501 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
    25561 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
    25621 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
    25681 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
    25741 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
    25801 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
    25861 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
    25921 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
    25981 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
    26041 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
    26101 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
    26161 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
    26221 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
    26281 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
    26341 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
    26401 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
    26461 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
    26521 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
    26581 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
    26641 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
    26701 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
    26761 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
    26821 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
    26881 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
    26941 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
    27001 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
    27061 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
    27121 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
    27181 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
    27241 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
    27301 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
    27361 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
    27421 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
    27481 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
    27541 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
    27601 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
    27661 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
    27721 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
    27781 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
    27841 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
    27901 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
    27961 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
    28021 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
    28081 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
    28141 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
    28201 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
    28261 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
    28321 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
    28381 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
    28441 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
    28501 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
    28561 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
    28621 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
    28681 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
    28741 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
    28801 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
    28861 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
    28921 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
    28981 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
    29041 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
    29101 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
    29161 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
    29221 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
    29281 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
    29341 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
    29401 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
    29461 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
    29521 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
    29581 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
    29641 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
    29701 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
    29761 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
    29821 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
    29881 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
    29941 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
    30001 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
    30061 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
    30121 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
    30181 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
    30241 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
    30301 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
    30361 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
    30421 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
    30481 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
    30541 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
    30601 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
    30661 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
    30721 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
    30781 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
    30841 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
    30901 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
    30961 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
    31021 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
    31081 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
    31141 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
    31201 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
    31261 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
    31321 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
    31381 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
    31441 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
    31501 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
    31561 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
    31621 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
    31681 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
    31741 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
    31801 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
    31861 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
    31921 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
    31981 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
    32041 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
    32101 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
    32161 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
    32221 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
    32281 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
    32341 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
    32401 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
    32461 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
    32521 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
    32581 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DACCZY010000018        32169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCZY010000018 DACCZY010000000
VERSION     DACCZY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.018"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="IVZ67_000675"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="IVZ67_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8164773.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="IVZ67_000676"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="IVZ67_000676"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8164774.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="IVZ67_000677"
     CDS             1947..2645
                     /locus_tag="IVZ67_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8164775.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="IVZ67_000678"
     CDS             complement(2813..5452)
                     /locus_tag="IVZ67_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8164776.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="IVZ67_000679"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="IVZ67_000679"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8164777.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="IVZ67_000680"
     CDS             7295..8470
                     /locus_tag="IVZ67_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8164778.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="IVZ67_000681"
     CDS             8474..9115
                     /locus_tag="IVZ67_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164779.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="IVZ67_000682"
     CDS             9112..10029
                     /locus_tag="IVZ67_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8164780.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="IVZ67_000683"
     CDS             10026..10697
                     /locus_tag="IVZ67_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164781.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="IVZ67_000684"
     CDS             complement(11238..11726)
                     /locus_tag="IVZ67_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8164782.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="IVZ67_000685"
     CDS             complement(11927..12193)
                     /locus_tag="IVZ67_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8164783.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="IVZ67_000686"
     CDS             complement(12193..12456)
                     /locus_tag="IVZ67_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8164784.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12449..12721)
                     /locus_tag="IVZ67_000687"
     CDS             complement(12449..12721)
                     /locus_tag="IVZ67_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164785.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12932..13117
                     /locus_tag="IVZ67_000688"
     CDS             12932..13117
                     /locus_tag="IVZ67_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8164786.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="IVZ67_000689"
     CDS             complement(13259..15082)
                     /locus_tag="IVZ67_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8164787.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="IVZ67_000690"
     CDS             complement(15680..18385)
                     /locus_tag="IVZ67_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8164788.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="IVZ67_000691"
     CDS             complement(18691..19638)
                     /locus_tag="IVZ67_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8164789.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="IVZ67_000692"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="IVZ67_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8164790.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="IVZ67_000693"
     CDS             complement(20273..20635)
                     /locus_tag="IVZ67_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8164791.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /locus_tag="IVZ67_000694"
     CDS             complement(20646..21767)
                     /locus_tag="IVZ67_000694"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8164792.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /locus_tag="IVZ67_000695"
     CDS             complement(21783..22133)
                     /locus_tag="IVZ67_000695"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8164793.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="IVZ67_000696"
     CDS             complement(22263..23774)
                     /locus_tag="IVZ67_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8164794.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /locus_tag="IVZ67_000697"
     CDS             complement(24096..25448)
                     /locus_tag="IVZ67_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8164795.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..26677)
                     /locus_tag="IVZ67_000698"
     CDS             complement(25601..26677)
                     /locus_tag="IVZ67_000698"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8164796.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26873..28618
                     /locus_tag="IVZ67_000699"
     CDS             26873..28618
                     /locus_tag="IVZ67_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8164797.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28898..29335)
                     /locus_tag="IVZ67_000700"
     CDS             complement(28898..29335)
                     /locus_tag="IVZ67_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8164798.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29319..30692)
                     /locus_tag="IVZ67_000701"
     CDS             complement(29319..30692)
                     /locus_tag="IVZ67_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164799.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31014..31502)
                     /locus_tag="IVZ67_000702"
     CDS             complement(31014..31502)
                     /locus_tag="IVZ67_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8164800.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31524..32126)
                     /locus_tag="IVZ67_000703"
     CDS             complement(31524..32126)
                     /locus_tag="IVZ67_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8164801.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6879 c         5940 g        10155 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    25921 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    25981 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    26041 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    26101 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    26161 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    26221 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    26281 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    26341 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    26401 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    26461 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    26521 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    26581 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    26641 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    26701 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    26761 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    26821 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    26881 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    26941 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    27001 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    27061 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    27121 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    27181 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    27241 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    27301 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    27361 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    27421 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    27481 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    27541 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    27601 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    27661 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    27721 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    27781 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    27841 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    27901 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    27961 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    28021 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    28081 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    28141 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    28201 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    28261 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    28321 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    28381 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    28441 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    28501 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    28561 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    28621 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    28681 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    28741 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    28801 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    28861 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    28921 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    28981 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    29041 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    29101 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    29161 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    29221 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    29281 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    29341 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    29401 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    29461 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    29521 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    29581 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    29641 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    29701 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    29761 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    29821 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    29881 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    29941 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
    30001 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    30061 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    30121 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    30181 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    30241 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    30301 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    30361 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    30421 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    30481 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    30541 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    30601 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    30661 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    30721 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    30781 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    30841 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    30901 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    30961 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    31021 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    31081 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    31141 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    31201 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    31261 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    31321 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    31381 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    31441 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    31501 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    31561 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    31621 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    31681 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    31741 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    31801 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    31861 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    31921 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    31981 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    32041 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    32101 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    32161 ctgcctcct
//
LOCUS       DACCZY010000019        32113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCZY010000019 DACCZY010000000
VERSION     DACCZY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.019"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            148..519
                     /locus_tag="IVZ67_000704"
     CDS             148..519
                     /locus_tag="IVZ67_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8164802.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(793..1485)
                     /locus_tag="IVZ67_000705"
     CDS             complement(793..1485)
                     /locus_tag="IVZ67_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8164803.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1488..2273)
                     /locus_tag="IVZ67_000706"
     CDS             complement(1488..2273)
                     /locus_tag="IVZ67_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8164804.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2510..3910
                     /gene="lacG"
                     /locus_tag="IVZ67_000707"
     CDS             2510..3910
                     /gene="lacG"
                     /locus_tag="IVZ67_000707"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8164805.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3935..4252
                     /locus_tag="IVZ67_000708"
     CDS             3935..4252
                     /locus_tag="IVZ67_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8164806.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4265..5938
                     /locus_tag="IVZ67_000709"
     CDS             4265..5938
                     /locus_tag="IVZ67_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164807.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5952..7583
                     /locus_tag="IVZ67_000710"
     CDS             5952..7583
                     /locus_tag="IVZ67_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8164808.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8074..9639)
                     /gene="guaA"
                     /locus_tag="IVZ67_000711"
     CDS             complement(8074..9639)
                     /gene="guaA"
                     /locus_tag="IVZ67_000711"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8164809.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9698..10621)
                     /locus_tag="IVZ67_000712"
     CDS             complement(9698..10621)
                     /locus_tag="IVZ67_000712"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8164810.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10817..11422
                     /locus_tag="IVZ67_000713"
     CDS             10817..11422
                     /locus_tag="IVZ67_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8164811.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11730..12746
                     /locus_tag="IVZ67_000714"
     CDS             11730..12746
                     /locus_tag="IVZ67_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164812.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12887..14188
                     /locus_tag="IVZ67_000715"
     CDS             12887..14188
                     /locus_tag="IVZ67_000715"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8164813.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14234..14896
                     /gene="deoC"
                     /locus_tag="IVZ67_000716"
     CDS             14234..14896
                     /gene="deoC"
                     /locus_tag="IVZ67_000716"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8164814.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14917..15315
                     /locus_tag="IVZ67_000717"
     CDS             14917..15315
                     /locus_tag="IVZ67_000717"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8164815.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15381..16463
                     /locus_tag="IVZ67_000718"
     CDS             15381..16463
                     /locus_tag="IVZ67_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8164816.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16675..18237
                     /locus_tag="IVZ67_000719"
     CDS             16675..18237
                     /locus_tag="IVZ67_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164817.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18230..19363
                     /locus_tag="IVZ67_000720"
     CDS             18230..19363
                     /locus_tag="IVZ67_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164818.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19363..20319
                     /locus_tag="IVZ67_000721"
     CDS             19363..20319
                     /locus_tag="IVZ67_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164819.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20418..21584
                     /gene="deoB"
                     /locus_tag="IVZ67_000722"
     CDS             20418..21584
                     /gene="deoB"
                     /locus_tag="IVZ67_000722"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8164820.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21608..22426
                     /locus_tag="IVZ67_000723"
     CDS             21608..22426
                     /locus_tag="IVZ67_000723"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8164821.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22443..23150
                     /gene="deoD"
                     /locus_tag="IVZ67_000724"
     CDS             22443..23150
                     /gene="deoD"
                     /locus_tag="IVZ67_000724"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8164822.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23448..24134)
                     /gene="gpmA"
                     /locus_tag="IVZ67_000725"
     CDS             complement(23448..24134)
                     /gene="gpmA"
                     /locus_tag="IVZ67_000725"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8164823.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24306..24887)
                     /locus_tag="IVZ67_000726"
     CDS             complement(24306..24887)
                     /locus_tag="IVZ67_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8164824.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25020..25703)
                     /gene="rpiA"
                     /locus_tag="IVZ67_000727"
     CDS             complement(25020..25703)
                     /gene="rpiA"
                     /locus_tag="IVZ67_000727"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8164825.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26022..26435
                     /gene="rpsL"
                     /locus_tag="IVZ67_000728"
     CDS             26022..26435
                     /gene="rpsL"
                     /locus_tag="IVZ67_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8164826.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26519..26989
                     /gene="rpsG"
                     /locus_tag="IVZ67_000729"
     CDS             26519..26989
                     /gene="rpsG"
                     /locus_tag="IVZ67_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8164827.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27083..29170
                     /gene="fusA"
                     /locus_tag="IVZ67_000730"
     CDS             27083..29170
                     /gene="fusA"
                     /locus_tag="IVZ67_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8164828.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29342..30529
                     /gene="tuf"
                     /locus_tag="IVZ67_000731"
     CDS             29342..30529
                     /gene="tuf"
                     /locus_tag="IVZ67_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8164829.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            complement(30708..32009)
                     /locus_tag="IVZ67_000732"
     CDS             complement(30708..32009)
                     /locus_tag="IVZ67_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8164830.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         9812 a         5912 c         7012 g         9377 t
ORIGIN      
        1 tatagaatca acactcagag aacgggttgg tttttaatag atttaataag tactgataat
       61 gaaatttctc ctggttggga aaacgaagtt ggttaccctg tagaagaatt ttttcaagat
      121 tactttgcgg agtatgccga taggataatg agagattacg taacagataa aactcaagaa
      181 gtcatcaatc aatcccagtc attaaatgaa ttatctgata aggcgaaaga agaagcagtt
      241 gccattattt cttcagggat tagtgactat attgatatga gtggtgaaat caattatggc
      301 gataaagaca aaattgtacg catgataaaa gcttttatca aaacttttga tgtgaaggca
      361 gatcttaagt ttaatgatac ttacctagta ggaaaattaa ttaatatcct tgggacagag
      421 aaagatacgg aaacgtttat ttcctctttg tctatgttgt ttacagataa gcatttttca
      481 actcagcttg gtggagagga actagtggaa atcttttaac cattacgccc agcattaata
      541 aaagtatatc aaaaaagtac tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa
      601 aacactattt ttaatgttaa ttatagatag agatgaacgc gtcatatggt taaaagaatg
      661 aaaaagtcaa agatacactg ctatatttca ctaaggatat agcagtgtat ctttcatgtc
      721 gtttcactgg cagtttttta taaaaggagg tgtgcatttc agttgctttt ctttggtagt
      781 tagttgaatc gtttaagaat taacggatgt ggcagacagg tgctctgtcc aaaaaccagt
      841 aaaatctttg atggttccat ctttatttag ctgtgcctgt tcaaatactg ttttgttttt
      901 accgaatgga tcaattgcaa taattgttcc gatccccgca cgtttggcag caagtaatcc
      961 tgagtaagca tcttcgataa caagacaatc agccggagct aagctcagtt tgtctgccgc
     1021 tttaagaaaa atatccggtt gcggtttccc agggaaagag ccgtcgtcat agacaatctt
     1081 gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt
     1141 gacagtagcc gtggcaatcg ttaatggaat ctgttggtca gtcagctggt caagagtcgc
     1201 ttcaagccct tttgtcaact gaagctcgtt ttcattttgc aagcaaagca tacgataata
     1261 gctttctttt tcttctgaca atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa
     1321 atgtttcaaa atctccgtat tcgtccgacc atgaatattt tttaaaatat cctcgtctga
     1381 taaaccatct tttgtatgat catgtatcat atgcagccaa gcagcttcat gcagatgaga
     1441 gtccaaaaac attgttccgt tgaaatcaaa tataatccct ttcatagtta cgctcctata
     1501 gtggtatttt cggttgaatc agcaatttgc agatcgacat attgtgcata gtgctcttga
     1561 acctcccgcg agatctggcg atccgtaatg agcagatcga aatcatgcaa attataaaac
     1621 acataaaaat catctcgatt gaatttttta ttatcaacga gtagatattt cttacgggaa
     1681 ttatttagag cgatctgctg tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg
     1741 ttgtgaacgc catttgaact gataaatgct tttgtaaact ttagtcttct taaattgtcg
     1801 ttggtgatcg gtccgacaaa tgcgccggta tttttccgga aatcaccgcc aatcagtaga
     1861 atttctggtc catctgcttg agcaagaata tcaaaaactg gataattatt tgtaatgatc
     1921 cgaattttct tgttcagtaa atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag
     1981 aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg cagcaatttc ttttttttct
     2041 tgaatctgca gagtggcttt ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga
     2101 ctttgagccc ctccatggac acgcagcagc ttgcccgatt tttctaattc atctaaatct
     2161 ctgcgaacgg tcatatcaga aacatctaat tgattgatga tttcattaac ggtaaggatt
     2221 ccttttttat tgaccatttc tacgatagta agcaatcttt cttttttcaa cattgtcttc
     2281 acctctaatt taaggataat ctatcatata aacggacaac agtcaatcta attcgaacaa
     2341 aagtaaacat ttagggtggt ttttatgttt gttacaatag attttattgc tgttttctgg
     2401 tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca
     2461 atcagttaga aagagcgaaa actgattata gatttgagga gagatgtaca tgcttaaatt
     2521 accagaagat tttatttttg gcggagccac agcagcctat caggttgaag gagccactaa
     2581 ggaaggtggc aaaggcgctg ttgcttggga tgacttttta gaggaacaag ggcgattcag
     2641 tcccgatccg gcaagtgatt tttatcatca atatgcaaag gacattgaat tgtgtgaacg
     2701 ttttggtgta aatggtctaa gattatccat tgcttggagc cgcatttttc ctgatggagc
     2761 tgggaaacca aatccagaag gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa
     2821 aagaaacgta acgccgtttg tgacgttgca tcattttgat acccctaaac gtttgtttga
     2881 ccatggagat tttttgaatc gagaaacaat agaagcattt gtttcctatg ccattttttg
     2941 ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt aatgaaatct atccagttgc
     3001 aaccaatcaa tatttgttag gtgttttccc accaggaatc aaatatgatt ttacaaagat
     3061 cgtcgcctgt cttcataata tgatggttgc tcatgcccgt gtagtgaact atttcaaaga
     3121 aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta gagacgaaat atgcggcaac
     3181 agacgcgccg gaagataagc atgctgcctt tttagacgat gcattgtcga tccgtttctt
     3241 attagatgct acctatttag gctattattc aactgaaacc ttgactgctc ttgatgagat
     3301 atgtgaagca aatcaggcca gctatcattt tcctgaagaa gatttcgttg aattgaaaaa
     3361 agcatccacg cgtaatgact acttagggat caatcactac caatgccatt ttgtcaaagc
     3421 ttacgatgga gaaaatgcaa tccatcataa cggtacgggt gaaaaaggga cctctgttta
     3481 taaagtgaag ggaatcggag aacgtattta taaagaagga attcctcgaa ctgattggga
     3541 ctggctgatt tatccagaag gtctatatga cttgttgctg cgaattaaaa gcgattaccc
     3601 acattacaac aaaatctata tcactgagaa cgggatgggg tataaagatc agtttgaaga
     3661 tggcattatt atggatcagc cgagaatcga ctatttgaga gtctatttgg aatccttaag
     3721 caaggcgata acagccgggg taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct
     3781 tttctcatgg acaaatgggt ataacaaaag gtatggcttg ttttatgttg attttgaaac
     3841 ccagaagcgt tacccaaaag aatcagctta ctggtacaag ttggtcagtg aaaccaaaac
     3901 gattatttaa taagtgataa tggaggaaga aaagatgaat aaagaggaat tacaaatgct
     3961 tggttttgag atcgttgcat attcaggcga cgctcgcagt acattattga aattattgaa
     4021 agaagtaaga caaggaaact ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa
     4081 tttaaccctt gcacataatt cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga
     4141 gatggagatg ggattcattt ttatccatgg gcaggatcat ttaatgacca ctttattgct
     4201 gagagatctg attcaagact ttattgtact gtacagacag aatcaaggat aggagaagaa
     4261 cgctatggat ggaatcatta aacaaatcga aaaagggaaa ccattttttg aaaaaatctc
     4321 acgtaatagt tatttaggag ccgttcgtga cggattttta acagcgatgc cagctatttt
     4381 attttcaagc attttcattt tgattgctgc ggttcctgag atttttggtt accaatggcc
     4441 tgaatctgtc tccacatggc tatggaaagt ctataattat tcgatgggtg ttgtagggtt
     4501 actcgtttca gcaaccactg ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa
     4561 aaacaaagta atcaacacca cctctgtgat gctggcctct gtggtaagtt tcctgttgtt
     4621 aagcgtctct ccgattgaag gtggattttc aacggaatat atggggacaa agggactact
     4681 ggcttccttt gtcgctgcat ttttaacagt taatttatat aagttttgtg tgctgaaaga
     4741 tattaccatt aaaatgccta aagaagttcc tggaacgatt tcgcaaacct tccgagatat
     4801 ttttcctttc tctttttcag tatttgcggc cgttttaatt gatttaggca ttcgacaaat
     4861 cttccaaatc tcatttgcag aagcaatcat tacggtgctg cagccgctat ttacagcagc
     4921 tgacgggtac ttaggaatcg ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg
     4981 tgttcatgga ccgtcgatcg tggagccggc aattgcggca attatctatg ccaatgtgga
     5041 aaccaatctg caattgttca attccggtga acaagcttct aaagtactga cggtgggttt
     5101 agggaatttt gtaggaacta tgggaggaac tggggcaaca ttggttgttc cttttatctt
     5161 cctactcttt gctaagtcta agcagttgaa ggcagttggg aaagcttcct ttattccagt
     5221 aagtttcgct gtcaacgaac cattgctatt tgcggcaccc atgattttga acccttattt
     5281 ttttattcca tttttgttaa cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga
     5341 tgtattgggc atgaacagct ttatgtacgt tttgccttgg gcaactccag caccgatcgg
     5401 tttggttcta ggaacgggca tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt
     5461 cgttgacttc ctgatctatc taccattttg caaagcatac gatgctgtct tagtagagaa
     5521 agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga
     5581 ggaagtacct gtggcagcga gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt
     5641 ggtactttgt gctggcgcag ggacaagtgg gatgttagct aatgctttaa aaaaaggtgc
     5701 atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct tatggttctc attacgaaat
     5761 gctgcaggat tttgatatgg tggtcttagc accgcaagtc aattcttact atgatgatat
     5821 caaagaagac accgatcgct taggaatcaa gctggttgcg acaaaaggag cagaatatat
     5881 cggattaacg agagatcctg aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag
     5941 gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt
     6001 taggggcttt gggggctgtt tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg
     6061 gagcagatat ttcgatcctg tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg
     6121 atatttcaaa agatgcctta gagattttaa aaaataatgg tgtcaactat gttcgactgc
     6181 gtttgtggca ggatccttat aatagtaatg gagaaagtta tggagcaggg acaaatgatc
     6241 tggaaacgac gattgacctt gcaaaacgag caaaaaatct gggacttaag gtgctgctgg
     6301 attttcacta cagtgatttt tgggttgatc ctgggaaaca gaatcttcct aaagcatggc
     6361 aaggtctgac ctttgaagaa atgaatacgg cattgtacga ctatacaaag aacgttctgt
     6421 cggaaatgaa gcagctggac gtttatcctg atatggttca gatcggcaat gaactgaata
     6481 gcggaatgct ctggccttat ggcaagtctt ggggagaagg cgggggagaa tttgatcgat
     6541 tggctgcttt tttgaagtct ggtatccaag ctgttcgaga cactcaacct aaaacaactc
     6601 cagtaatgct ccacttagct gatggtggcg atacaggagc atttacttgg tggtttgatg
     6661 aaatcacgag tcgaggtgtg tcatttgatt taattggagt gtcgtattat ccgtattggc
     6721 acggttcaat gaatgaactt caaaccaata tgaatgcgat cagccaacgg tatggtaaag
     6781 aagtgatcgt agttgaaaca gcttatgggc atacaacagc gaatgccgat actatgccaa
     6841 atgcttttgg tgaagccgaa gcagctgcgg ggggatatga accaacaccg acaggacaag
     6901 cagagtattt gctggatctt gccgatcgga ttcaggcagt accgaataat cgcggggcgg
     6961 gcttcttcta ttgggaacct ctttggtaca acggaaatgt gagttgggca acacaagcag
     7021 gtatgaatta tctcagtgtt caatccacta tgggaaacga atgggacaac caagcgatgt
     7081 ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa
     7141 taaatctagt taaaaatgca agtttcgaaa gcgatggacc aacgaccagt ccttccaatt
     7201 ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat caaaacggaa gcgggcactt
     7261 tttttgggga ctacaaacta actttttggc aggatcaagc atacgaggca tcagcttatc
     7321 aaaatgttag tggcataagc aaaggaactt acacattgag tgcctatgta atgtctgggg
     7381 gagagtttga tactaatcag ttatatatca aaaattatgg tggagcggaa ctaaatactg
     7441 cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa agacattcca attacaacag
     7501 gtgtttgtga aattggggtt tatagcaagg cttcaggaaa tgcttggacg aacgttgatt
     7561 ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga ttcaagatcg gaggttccct
     7621 tcagccacat cgttagcgta gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa
     7681 taaccagaaa cgatttccag tcatttttac caaaacgagt aaagcaccag acttcatcta
     7741 cactttgtat tatgctaaag agagttcaac gatttcgtaa gaagcaatga aaaaattgca
     7801 aaaagttgtg cagataacat aaagtttgtt taagaaaggg taaccttaga attccgtcgt
     7861 gaatatgatt tgagcactta gatggataga aaaaagttct cggaaaacag ttcgtttttc
     7921 gagaacttct ttttatgata agaatgaaac aaattaactt atattatagt ctcttttggt
     7981 agggaaccat cttattttta atgaaaatca agcattttag ctattgaaga atgttgattt
     8041 atagggattc cccctccata ttatgttggc tctttattcc cactccactg ttgctggtgg
     8101 tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac
     8161 agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat
     8221 tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc
     8281 gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc
     8341 agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc
     8401 agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac
     8461 gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt
     8521 gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg
     8581 agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc
     8641 ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac
     8701 atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa
     8761 cttgtctaag aagcgatctc ttgcattgac tttgatgatg ttcaagccga attttccgcc
     8821 tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat
     8881 agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac
     8941 gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac
     9001 ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc
     9061 aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat
     9121 accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc
     9181 gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt
     9241 ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc
     9301 tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa
     9361 ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat
     9421 cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg
     9481 attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc
     9541 aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat
     9601 aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt
     9661 aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg
     9721 aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca
     9781 agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa
     9841 aggcttcttc acgatctccg atagcaaaac gataataata attctctgga ttttggaaag
     9901 ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg
     9961 catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta
    10021 attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact
    10081 tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac
    10141 cagattttac acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc
    10201 ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag
    10261 tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc
    10321 tggctgttgt actttttcca acggctacac ttccggcgat cccgataata aaaggcggta
    10381 cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca
    10441 tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa
    10501 tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat
    10561 tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca
    10621 tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc
    10681 ccaccactta agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga
    10741 gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat
    10801 gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga
    10861 ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg
    10921 tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg
    10981 ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg
    11041 gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc
    11101 ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca
    11161 atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc
    11221 gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa
    11281 gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa
    11341 gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat
    11401 catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact
    11461 gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa
    11521 gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa
    11581 taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta
    11641 tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata
    11701 gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa
    11761 tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta
    11821 gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt
    11881 atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg
    11941 tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg
    12001 gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac
    12061 aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg
    12121 agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg
    12181 gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag
    12241 ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg
    12301 gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc
    12361 tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg
    12421 catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca
    12481 tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct
    12541 cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag
    12601 tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg
    12661 aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga
    12721 gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa
    12781 aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc
    12841 ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga
    12901 tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt
    12961 cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat
    13021 tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc
    13081 tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg
    13141 tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac
    13201 agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga
    13261 agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga
    13321 aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta
    13381 tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat
    13441 gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg
    13501 tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg
    13561 gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga
    13621 agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc
    13681 agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa
    13741 agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc
    13801 gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca
    13861 tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt
    13921 tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg
    13981 taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg
    14041 tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac
    14101 ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac
    14161 gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa
    14221 aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc
    14281 atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt
    14341 ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc
    14401 cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga
    14461 aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc
    14521 tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc
    14581 aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat
    14641 catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg
    14701 tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc
    14761 agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat
    14821 cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac
    14881 tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg
    14941 atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta
    15001 ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca
    15061 tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga
    15121 gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt
    15181 ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta
    15241 ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact
    15301 gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta
    15361 ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac
    15421 tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg
    15481 gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg
    15541 accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac
    15601 tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc
    15661 caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac
    15721 taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg
    15781 atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt
    15841 cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg
    15901 gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg
    15961 caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct
    16021 ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca
    16081 tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg
    16141 aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg
    16201 aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg
    16261 gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac
    16321 acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg
    16381 aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc
    16441 cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca
    16501 aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt
    16561 ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg
    16621 tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt
    16681 caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca
    16741 aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac
    16801 ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg
    16861 aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta
    16921 gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac
    16981 atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa
    17041 gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt
    17101 gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca
    17161 gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta
    17221 atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag
    17281 ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc
    17341 ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga
    17401 acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt
    17461 gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag
    17521 gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg
    17581 atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa
    17641 gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa
    17701 gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa
    17761 acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa
    17821 catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca
    17881 aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat
    17941 cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt
    18001 attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc
    18061 gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt
    18121 gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc
    18181 tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg
    18241 cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt
    18301 aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct
    18361 agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt
    18421 gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct
    18481 ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga
    18541 tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat
    18601 ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac
    18661 aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc
    18721 tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg
    18781 gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc
    18841 aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat
    18901 ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac
    18961 gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg
    19021 attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg
    19081 aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt
    19141 gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga
    19201 gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg
    19261 tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga
    19321 atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta
    19381 attgccttga tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta
    19441 ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc
    19501 atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg
    19561 acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac
    19621 gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg
    19681 atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat
    19741 aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt
    19801 ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc
    19861 ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa
    19921 aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta
    19981 ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt
    20041 aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt
    20101 ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc
    20161 ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa
    20221 attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt
    20281 ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg
    20341 tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa
    20401 agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa
    20461 ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca
    20521 ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc
    20581 gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag
    20641 aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca
    20701 aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag
    20761 aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg
    20821 attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg
    20881 tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc
    20941 aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc
    21001 cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga
    21061 atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg
    21121 ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa
    21181 gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca
    21241 gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct
    21301 atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag
    21361 aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg
    21421 atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg
    21481 cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt
    21541 cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag
    21601 aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa
    21661 aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga
    21721 tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac
    21781 agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc
    21841 catgcaaggg agattccact actatgaagg tcattccatg caaacagtga cctatccagt
    21901 acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt
    21961 caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg
    22021 tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag
    22081 tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt
    22141 ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga
    22201 gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt
    22261 catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc
    22321 agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa
    22381 acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata
    22441 aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg
    22501 gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca
    22561 atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc
    22621 aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt
    22681 atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg
    22741 ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt
    22801 tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga
    22861 tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct
    22921 acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa
    22981 tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga
    23041 tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta
    23101 cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg
    23161 aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt
    23221 tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt
    23281 gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
    23341 ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa
    23401 aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta taggtagtat
    23461 tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc tgttggaatt
    23521 tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag ggcgcgtaat
    23581 gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg agcaatttca
    23641 tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc accaggaata
    23701 tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc gtcagtttct
    23761 tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg
    23821 tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg acgttcgttt
    23881 aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa gatcaagtta
    23941 catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc gattccagct
    24001 tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc tagatttaca
    24061 tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg acgagaaaaa
    24121 actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg aaattttcag
    24181 ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag ataagattct
    24241 atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt
    24301 aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata tttataacat
    24361 gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa
    24421 tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt
    24481 ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc
    24541 atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt
    24601 ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt
    24661 aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt
    24721 tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca
    24781 tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt
    24841 aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc
    24901 tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag
    24961 atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt
    25021 tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta
    25081 gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta
    25141 gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca
    25201 tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct
    25261 tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac
    25321 ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac
    25381 gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca
    25441 gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc
    25501 tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct
    25561 gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg
    25621 gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg
    25681 ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct
    25741 ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa
    25801 agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa
    25861 caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata
    25921 tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg
    25981 tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat
    26041 tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag
    26101 gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag
    26161 caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac
    26221 gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact
    26281 tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac
    26341 gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc
    26401 gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact
    26461 tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg agttacggat
    26521 gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa
    26581 attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa
    26641 cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt
    26701 gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg
    26761 gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct
    26821 tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc
    26881 aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac
    26941 tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg
    27001 tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt
    27061 aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat
    27121 cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta
    27181 tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat
    27241 ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa
    27301 gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga
    27361 acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga
    27421 acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt
    27481 tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga
    27541 ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac
    27601 aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga
    27661 gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa
    27721 attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga
    27781 agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt
    27841 ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc
    27901 tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa
    27961 aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt
    28021 taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt
    28081 tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg
    28141 tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat
    28201 cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag
    28261 tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc
    28321 taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga
    28381 tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg
    28441 tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa
    28501 cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg
    28561 taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac
    28621 accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc
    28681 tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt
    28741 agctggctat ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt
    28801 cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa
    28861 gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga
    28921 ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga
    28981 agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg
    29041 ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga
    29101 ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa
    29161 agctgaataa tttcttgaaa ccggatagtc ctgcaaagag attatccaaa gagactatat
    29221 ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg
    29281 tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa
    29341 aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca
    29401 cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa
    29461 cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg
    29521 aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt
    29581 tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga
    29641 cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat
    29701 cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat
    29761 ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga
    29821 atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga
    29881 aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat
    29941 cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc
    30001 aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg
    30061 tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt
    30121 tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact
    30181 acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat
    30241 cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg
    30301 tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac
    30361 aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat
    30421 ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga
    30481 aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt
    30541 tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt
    30601 tgttccgagc ttttttattt attgcaggat caggaaaaaa gggctctttg tcaaatagga
    30661 ctgatagagt gagataagaa catctaaggt agaattcctt ttctacttta gatgtttttt
    30721 gattcatagg taatcagatt atttagtaag aaaattctgg tcttcatttt ccgaaagctt
    30781 cgaaagccat aagcgactcg tttcaatgct ttgatgtggg tatgcagatt ttctattttg
    30841 gcatttgaat aaggcaattc gagggctaaa cgaatgccgg attcatagtt tataaaagct
    30901 ttttttatag aatgcagttc ttttggaacg gaattgggca tcgttcttac aagtttaaag
    30961 aaagtgtccg cctctttgtc gtgaaacgct tgaagaattt cttgatagta aaaataggcg
    31021 attcgtaatt cgtcagagat cgaaagcaga cgatccacca cttcactttc ggtgagatac
    31081 gcccaaccaa aattgcgcca tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg
    31141 atcagaagcc gagacaacgc ctttaattgt cgataatatt tggcttgtgt tggatcgttt
    31201 cgatcaaagc ttttcatgat tcgtacgcga atatcctcaa atttacgaat gagatgtttg
    31261 acaatgtgaa aacgatccac aacgagtttt gcctttggaa aacattcctt tatcagagag
    31321 gcataagaaa cattcatgtc cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca
    31381 aattgcataa aataagaaac gagagccttt ttcttccgtg aaggaagaat atccagtaat
    31441 tttccagtat cgccatccat acaagaaaag gagtagggac cgacttgatt tttcagtgaa
    31501 cgaaattcat ccattaataa acaacgcggt aaccaatttc tttttacttt tgtcatgggg
    31561 atcagagaag cgagtgtccg agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt
    31621 gtcgtcattg cttgtttttc agttagctct tctaaaattt tatagcgcac ttgtttagcg
    31681 ataaagcagt ttctatcgac aatgtaggta ttcgccgtaa aataggtgtg gcagccacga
    31741 cagtggtagc gttgtttcgc taaagcaaga taagtcagtt ggttcgcaca tttattaagt
    31801 tgaatgagcg attttttctt tccgttctta acgatcatgc cttcatgatt catctgttta
    31861 cacaaaggac agcgacgaat ttctttactt aacgtcgcag aaacaacaag actcttgata
    31921 ttcttaatgc tcttcgattc cacatttgtg atgaaaatgt ttttatcttt gattccaagt
    31981 agtagtttga tatgatgaag atcaggcata cagaacgctc ctttgattgt gttgtggtga
    32041 ttcaattcta acggagcttc tgtatgtttt gttttttcat gctcaaaagg gaccgataga
    32101 atgattttct atc
//
LOCUS       DACCZY010000020        30977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCZY010000020 DACCZY010000000
VERSION     DACCZY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.020"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(29..1801)
                     /locus_tag="IVZ67_000733"
     CDS             complement(29..1801)
                     /locus_tag="IVZ67_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164831.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1755..3509)
                     /locus_tag="IVZ67_000734"
     CDS             complement(1755..3509)
                     /locus_tag="IVZ67_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164832.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3619..4188)
                     /locus_tag="IVZ67_000735"
     CDS             complement(3619..4188)
                     /locus_tag="IVZ67_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8164833.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4206..5630)
                     /locus_tag="IVZ67_000736"
     CDS             complement(4206..5630)
                     /locus_tag="IVZ67_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8164834.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5632..6300)
                     /locus_tag="IVZ67_000737"
     CDS             complement(5632..6300)
                     /locus_tag="IVZ67_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8164835.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6430..7014)
                     /locus_tag="IVZ67_000738"
     CDS             complement(6430..7014)
                     /locus_tag="IVZ67_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8164836.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7345..7578)
                     /locus_tag="IVZ67_000739"
     CDS             complement(7345..7578)
                     /locus_tag="IVZ67_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8164837.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7593..8543)
                     /locus_tag="IVZ67_000740"
     CDS             complement(7593..8543)
                     /locus_tag="IVZ67_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8164838.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8540..9382)
                     /locus_tag="IVZ67_000741"
     CDS             complement(8540..9382)
                     /locus_tag="IVZ67_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8164839.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9384..10103)
                     /locus_tag="IVZ67_000742"
     CDS             complement(9384..10103)
                     /locus_tag="IVZ67_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8164840.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10795..10953
                     /locus_tag="IVZ67_000743"
     CDS             10795..10953
                     /locus_tag="IVZ67_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8164841.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11025..12251
                     /locus_tag="IVZ67_000744"
     CDS             11025..12251
                     /locus_tag="IVZ67_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8164842.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12339..12731)
                     /gene="rpsI"
                     /locus_tag="IVZ67_000745"
     CDS             complement(12339..12731)
                     /gene="rpsI"
                     /locus_tag="IVZ67_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8164843.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12747..13190)
                     /gene="rplM"
                     /locus_tag="IVZ67_000746"
     CDS             complement(12747..13190)
                     /gene="rplM"
                     /locus_tag="IVZ67_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8164844.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13709..14176)
                     /locus_tag="IVZ67_000747"
     CDS             complement(13709..14176)
                     /locus_tag="IVZ67_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8164845.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14296..15834)
                     /locus_tag="IVZ67_000748"
     CDS             complement(14296..15834)
                     /locus_tag="IVZ67_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8164846.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15914..16600
                     /locus_tag="IVZ67_000749"
     CDS             15914..16600
                     /locus_tag="IVZ67_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8164847.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16801..17328)
                     /gene="msrA"
                     /locus_tag="IVZ67_000750"
     CDS             complement(16801..17328)
                     /gene="msrA"
                     /locus_tag="IVZ67_000750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8164848.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17412..18089)
                     /gene="pgmB"
                     /locus_tag="IVZ67_000751"
     CDS             complement(17412..18089)
                     /gene="pgmB"
                     /locus_tag="IVZ67_000751"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8164849.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18086..19216)
                     /locus_tag="IVZ67_000752"
     CDS             complement(18086..19216)
                     /locus_tag="IVZ67_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8164850.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19216..21399)
                     /locus_tag="IVZ67_000753"
     CDS             complement(19216..21399)
                     /locus_tag="IVZ67_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8164851.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21396..22421)
                     /locus_tag="IVZ67_000754"
     CDS             complement(21396..22421)
                     /locus_tag="IVZ67_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8164852.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22466..23293)
                     /locus_tag="IVZ67_000755"
     CDS             complement(22466..23293)
                     /locus_tag="IVZ67_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8164853.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23290..24357)
                     /locus_tag="IVZ67_000756"
     CDS             complement(23290..24357)
                     /locus_tag="IVZ67_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8164854.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24383..25219)
                     /locus_tag="IVZ67_000757"
     CDS             complement(24383..25219)
                     /locus_tag="IVZ67_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8164855.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25216..26085)
                     /locus_tag="IVZ67_000758"
     CDS             complement(25216..26085)
                     /locus_tag="IVZ67_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8164856.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26155..27438)
                     /locus_tag="IVZ67_000759"
     CDS             complement(26155..27438)
                     /locus_tag="IVZ67_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8164857.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27452..29137)
                     /locus_tag="IVZ67_000760"
     CDS             complement(27452..29137)
                     /locus_tag="IVZ67_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8164858.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29412..30437
                     /locus_tag="IVZ67_000761"
     CDS             29412..30437
                     /locus_tag="IVZ67_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8164859.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9447 a         6199 c         5052 g        10279 t
ORIGIN      
        1 attgtaggtt aatttaaagc ctatttcatc atcgtgattg agcttctttt tcatagacga
       61 atttctcaga tagtttagtt ttctttatga gatttttgta tatttctgac cttcgataca
      121 actcattatg agtaccttga tcaactagtt ttccttgatc catcactaca attttattta
      181 ccttttcaat agacttcaag cgatgcgata taattagtac tgtcttatct tgtattaaac
      241 gatttaaact ttcctgaata cgtttttcat tttccgcatc taaactggca gtaatttcat
      301 ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct
      361 ctcctccaga aagtgaactt ccgttttctc cgaccaatgt atgataaccg tcaggtagtt
      421 tttcaataaa gtcttcgcag ttagctaaac gagctgcttt tttcacttcc tcatctgacg
      481 cttgactatt tccaatacgt atattttcca aaatacttgt attaaacaaa gttacctctt
      541 gaaaaacaat agcgattttg ctaaacaaag aagctggaga tacacgttta atatcatacc
      601 catcaattaa aatacagcct tcatcataat catacagtct agacactaat ttcaagatac
      661 tagttttccc ggaaccacta gcacctacta acgcagtcac ttctccttgt ttagccgtaa
      721 aagagatatg atctaaaatt ggggtattgt tatcatatga gaaagagaca tctcgcaact
      781 caatgtcaaa agatttcaga ggactatctg atccctcttg gatactggtt tccttcatag
      841 ctctaattcg ctgtatttta ggagctaaat aaaatatttc aagcactgct tctttcatag
      901 aatcgaagga atcctttatt tttattgcgg ctagtaagta accaacgacg tagagtatag
      961 tcacttcacc cgttaacagt aagtgaacac ctactagaat gactactgct aaactaacat
     1021 atgagaaaat agaggataag gccatgactg aaaaagtact taattctacc tttaaatgaa
     1081 tcctttcact ctcttccatt tttttgaaaa gtctgtcttg aactttttta gatagattaa
     1141 agctattaat ctcttgctgc aattctatag tttcttgaaa ttcttccgag ttctctctca
     1201 aagtatcgta atatttagta ttggctttcg tttgggattt ctttgataac aagattaaga
     1261 caaaactaaa taacgttggc aaaataacag ctaatcccat tttgacatta ccaaccaaaa
     1321 gcatcactga aataaaaggg aaaaacagag ccataccgcc ggctttaggt attgcatgac
     1381 tcatcgcatg ctcaatacct tcaacgtcag acatgatagt ttgagataaa tctgataaat
     1441 tatgttttga aaaataggat aatggtaaat ttgacaaatc gtctgcaatt tgcactctta
     1501 aatgcgcact ttctttataa gtactattat ataagttttc atactcccta tctaacaacc
     1561 aatacatgaa aagcaaagta acaaacgata cggcaaaata aagccaacgg ggctttgtat
     1621 tttctaaaac taattcgtct atcagcatca ttataatgaa tgctggtacc atattaatgc
     1681 agtatacaaa aaaactagaa atagaagctt tagttaaagc ttctgcgccc tgatcactta
     1741 aagcaaattt gtttttataa aaacttttca ttgtcaagcc tccaatcatt tgcattctca
     1801 taattagtga ccaattgttt atacacttga tgtttagcca ttaattcatt atgatttcca
     1861 cgttctacta ttttcccttc tgacaagaca agaatttcat ctacagattt aatgctggat
     1921 aatcgatgag ctatcataat aactgttttt tctttcatca gatgttgaaa agccttttgt
     1981 agttcatact cattatctgg atcaatagat gcactggctt catccataat aacaatcttt
     2041 gcatctttta aaattgctct ggcaatagct atacgttgtt tttctcctcc tgacagataa
     2101 actcctttac taccaattaa ggtatgctct ctttcgggaa atttttctaa aatcgattcg
     2161 caaccggcta atcttagcgc gtcaaatact ttacttgctg gcgcttgagg atttgctata
     2221 gaaacgttat cgtaaatact ctgattgaat agtttcgtat cttgaaagac aaaagcaatt
     2281 gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct taccaccaat tttaatctgt
     2341 cccttatcca cattgtagaa acctgaaatt agtttagcta tcgtactctt tccgcttcct
     2401 gattttccta ctaatgcata aattcttttt tgttctaaaa ctaagttgaa attttctaaa
     2461 acacactctt ctttgtatga aaagctgaca ttttcaaatg caatgtcaca atttttaatc
     2521 tgtgtctcgt tcccatattt tagttggtct tgcttcattt ctaaataaag ggagtctagt
     2581 gtttcgactg catatcttgc ttcaaacaag tgcatactta catacattat tctcatgaaa
     2641 gaaacaaaaa taattccact taaaaaaaac aacatcaaca agtcaacagc taatatcttg
     2701 ggagtggtaa gagaagttat aaaaaaggtt ataggaacta tcaaaatgca aactatgcca
     2761 aaaaatagcc attgaaaaag aacaaaaggt gttttcccac tcaaagaata cttatatgca
     2821 aacttagcat aatcgttaat tgctttattc aaggctttaa atgatgttac tttagcacca
     2881 aatatcttta taacttgaat tccgcggata tattctaccg tttcagagct taacacatct
     2941 aacgattctt gatatttttg aataaaattt gtattctctg tcattttctt taacaagaat
     3001 acaccaatta ctaacatggc gattagaacc aatccaattt taaagttaat gaaaaagcct
     3061 aaggcaactg ataaaacagg tgttactatg gcttgagcac tatctggaat caagtgagca
     3121 attgccatat gtgttttagc cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa
     3181 tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc taattccctt ttttctcaaa
     3241 ttagtctcta agcgaaaccc aaggatatga gaaaataagc cagaaaaaat ataaaataaa
     3301 gctcctaaag ttaaccaagc aacagtttga accgcataac tcttagctaa atattcatct
     3361 cctacaataa ttagagactg aagaaatttg taaatactgt aatatccata gactgttaat
     3421 acgacagaaa aatcagaaaa taaagtggct aaccatccgt agtatttcat tttagggaca
     3481 taactaaata attttttgta tactttcatt ttttcctccc ccgcattcaa tttcttctct
     3541 tattcgttta caactgttaa tgctttccgt aatagcgtaa attagagatg tacaaacaga
     3601 taaaaggtaa actacgttct attttttgta tttttttaag acttttcttc caatcaatac
     3661 accaatcata gataaacttg ctacaactaa cactgaaatg attccccata tacctaccgc
     3721 atagtggatc attgcatcca ttgttgcagc agattgtcct ttatctatcc aatcttgacg
     3781 aaaacgatct gtaaagaacc atataggaat ccactgtccg gcagcactgc ctaattgcat
     3841 tagagcccaa gacgcaactt gaggaattgc tcgatcatat ccaaatacag atagaataat
     3901 atcagctaag atcgccatac taatcgaact gataaaccaa ggtaagtacg ccccgcccat
     3961 taacgtaaaa aataacaata aaacaattgt gtaaatagta aaaattcctt ttctagggta
     4021 gttatagcta atataaacaa atatgattcc agataataat ccaaaaatcc cgggggatat
     4081 gctgtgacca aacgccccac caatactact aaccattatt gaaaaaaagt aaattactag
     4141 ggaaataact gtagctaaac caataaaaat ataatctttt gttttcatat atattctcct
     4201 caattctatt tcgttatgtc attaaaaatc tgttctatca aagttgaatc ttctactcta
     4261 agttttatat ctttttttat tccaccattt tttaaataaa taattctatt gcatgtttta
     4321 taaagaaatt ccggatcatg agtagctata actactgcac ccttttcttt taagtcttta
     4381 ataaggtcag caatcctttc catattaata taatccaacc cacttgtagg ttcatcaaat
     4441 agaaataaat tagtttttga caatgaagct atagaaacca ataatctttg tttttctcca
     4501 ccggataaat caaacggtaa ttgatctttc attttataaa gttccatttt ttttaaaatt
     4561 ttttcttgct gatctttact aactatttca ttccccaggg ctaactcgct cctcactgat
     4621 tctgtgaaaa attggtaatc catttcttgc attactaaaa acggcgatga caaatttttt
     4681 ttattttcga ttatctttcc tttggtaggg cgaataatct tcatgagtat tcttagtaag
     4741 gttgttttcc ccacaccgtt tttccctacc aaaccaataa catctccaac tttgacatca
     4801 aaatttatgt cttttagaat attttcataa gtaatttctt caacttttaa tacatctgca
     4861 cttacctttt cgatataggg aacttcaatc gaactattaa aaatgtcaaa tttccttaat
     4921 gcgttattgt tttcagtttt taacaactga atagttgtag tttttacagt tctattttta
     4981 acaaatagaa cttgatcaat taattctttc aaatagtaaa aacgatgttc agctataaca
     5041 atagtaaatc ctttattttt taaatcacaa agtaagtcag caagtttctt agtcgctttg
     5101 aagtccaaat ttgaagacgg ttcatccaat ataattaatt tagagtttag cgataaacta
     5161 catccaatag aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat
     5221 atatttgaaa gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt
     5281 gtaaatccaa agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat
     5341 tgtcctctag gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag
     5401 gattgtagcg agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt
     5461 ggaataagtc cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta
     5521 atcaatgtaa gactgccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta
     5581 acatccttat atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa
     5641 aatacttaag aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt
     5701 tgttttatag taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc
     5761 ggtcatttct tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa
     5821 atattgcaga ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat
     5881 cattcttatt tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat
     5941 tatttttttc ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac
     6001 actaaccaac aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga
     6061 tgtcgcaaaa aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa
     6121 aaacagaact gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa
     6181 tactaaaact gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa
     6241 aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac
     6301 tctttcacct ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt
     6361 attcagataa cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc
     6421 tttttttaat taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct
     6481 ggttaatagc atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc
     6541 gtagaatgag taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat
     6601 cattccgata gtctttaact tatctagaca acagactgtc tttttcgtca caaatgatgt
     6661 aattttttca acaatatctt gatatttggt tccagctgat tggaataaaa tcaaatcgaa
     6721 tatacgttta ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct
     6781 ccaacttttc tctatatcta tattttctaa gtttaagagg tatttggtaa actcttcatt
     6841 atacatatta ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt
     6901 agaagtaaaa tgtttataaa aagcacccgt agttacgcct gcggtcaggc acagttgcct
     6961 tatcttagtt tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaactt
     7021 ttggtgagtt ataatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt
     7081 aactttatta tcttacacag gacttgaagt ttcaatcgtt aacgtttatt actgaactca
     7141 ttccttagag ctaaactaat gagaaagagc agaataactg atacaaaaag caatcctaca
     7201 agaattaata gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa
     7261 aaaaattata tccagctatt aggataagtc ctatcataat cactccaata gccattttct
     7321 tatcctccct aaaaataaag tcttttactt aaaacctttt ttgtaatcgt caataaattt
     7381 tttcccttta tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagc
     7441 cagcatctca cttacagatg ctggactaac acttagaact tttgctaaat cattattttt
     7501 taatttcttt tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt
     7561 cgttttaatt tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta
     7621 tccaggttcc atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt
     7681 gaatcagtaa aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt
     7741 ggtctatcat ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt
     7801 tcaactaagt gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg
     7861 taagcagaag gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca
     7921 atcatttttt tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa
     7981 tccaattttt caatatactt atctagattt tctttataga attttttatt atcaggatct
     8041 ttttccgcta actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac
     8101 caagcatgag gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg
     8161 ccatcacttg ctgcaaaata gtctttgttt tcctctttgt tcgcattttt aactaatttt
     8221 gtaaaccaag catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct
     8281 tttgaagttt tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata
     8341 ggtacgatac tgtgtaaatc aattttatct tttgctatat tttcggtaat atctgctaaa
     8401 atcgaattag taactactac ttttaatttt tcgttcgaag ccactttatc tttgtcttta
     8461 ctatttattg aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt
     8521 actgctttcc attttctcat cataacgatc tctccttctt tcttaaaaat ttttgttttg
     8581 gtgagattag aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg
     8641 ctgctatgtt aaaactataa ccaataaaca atccaagcaa tgatgaactt gctccgactg
     8701 ttgcagaaat aaatatcata gttttcaagc tatttgcata taaatatgct gtagctgctg
     8761 gtgtaattaa cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta
     8821 ctaaagttaa tagaaccatc aatagataat gataaaaact gacattcatc cctattgctt
     8881 ttgccattaa tggatcaaaa gaagtgatta ataattcttt aaaaaataga actactataa
     8941 ccaacactaa aaccgaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa
     9001 tatttccaaa taaaatatga aataaatccg ttgagctatt tgcaactcct ataaggataa
     9061 ctcctagtgc taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac
     9121 tattgctttt tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa
     9181 taaaaaaatt aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa
     9241 tagcatcacc cataagagac attcctctta aaataataaa acaacctact gttccagaaa
     9301 caatccctat aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac
     9361 cattaataaa attttgaagc atattattcc ccctctccaa taaatatagt atctccatag
     9421 gcttttttta aattttcttc attaaataca gattctacac ttccgtgagc aattagattt
     9481 cgattcaaaa gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca
     9541 attagcactg tttttccctg atgtttcaat ttttttaacg tatccataat tattcgttca
     9601 ctaactaagt ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca
     9661 agacatcttg ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga
     9721 ttagaatatt ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca
     9781 tttttaattc tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt
     9841 acttttattg gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc
     9901 agttttttat tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt
     9961 aacattgctt taagcaatgt ggattttcca gcaccatttg gaccaataat acctgttaaa
    10021 gcaccttctt gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca
    10081 ttaagctttt tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc
    10141 atatggtata caaaaattat attttagtca accctaaatt ttatagttta gatttttttc
    10201 tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta agtagctttc
    10261 aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact actttattgg
    10321 cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata agcatcaaaa
    10381 catttataaa aattattcaa gactatgtct gttccatata caaatatgaa aatcagtaaa
    10441 ctccatttaa cttttttcgt agtgtgtctt tttatacatg tttatcgtta ttaatagtga
    10501 ataggaaaaa aactattttt gatcattgac aattaaatac aatttttgaa taacgatccc
    10561 tgctattcga gctagaaact caagtacgcc atgacctgat tagcgacaaa tacttagaga
    10621 tttctttttg ttctgggaaa acaaaatgcc atgacaatag cagcaaataa tgatacttcc
    10681 gtaaggctcg ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcaattc
    10741 aaaaatttca taactactat tttaggagat gattttatag aatccagagt ctacctgaat
    10801 aaaaaacaaa cctgtgcatt tctaggaatc gataataata ctctagataa gtggattcgt
    10861 atatataatt tcccagtaat aagagttggt ggtgtagttc gctttgacgt agaagatatt
    10921 aaagacttta tgaatacatt taaaataact tgaaattttc ttctcgccgc atatactaaa
    10981 attagtgaaa tatactgcct tacttttttt aaggagtgag gattttgcct acaaagcttt
    11041 caaatgggaa atataaaact aatctgagat atccaaaaag gtttaaagaa ataacaggaa
    11101 ttgcatcaga aaagtatcaa aaaacatttc caaataggca attggcgatt aaagccgaaa
    11161 atgatatgaa gaagaaaata gaaaaagttt tgagagagga gaatgcaaac tcattagaat
    11221 taaaaggaaa aataacgttt aaaaaatttt acgaatctaa atggcttcct agatatgaat
    11281 taggtcaaac aattagatca aacaggcctc ctagtgatat aaccattagt aatacaaaag
    11341 atatatttcg gttgcatatt ttacctatgt ttggtgaata cgcaatgaat tatctaaata
    11401 tcaatactga gataatatca gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa
    11461 aaatcataaa aggatatgtt cgttccatgt tcgatattgc agaaatacta aattatatcg
    11521 aatttaatcg aactactaaa attattcaaa gtatcactgc acctaaaaaa aatgctttag
    11581 aagaaaaaag aatacaagaa ggtaagcaag cactatcatc aaaagaactt actaattgga
    11641 tagaagcagt aaatgatgat tttaataatc atttacttac ttttcatgat tacacacttt
    11701 ttatgattac cctgtatctt ggtgatagaa aaagtgagac ttacgctttg caatggaaat
    11761 atattgattt tgaaaaacaa acggtccgtc ttaaacatac tttagataaa tatcaaagaa
    11821 aaaagtttac aaaaggtcga aaagataccg tcattcaagt accagaagtt gtaatgacat
    11881 tgctaagtga atggaaatcg gtacaagctg atcaattgtt gaaattaaaa attaagcaaa
    11941 cgttagatca gtatctcttt acttatacta aaccatctgg agaagtaaat tgtcctgttc
    12001 atgccgatta cctaaactat cgaatcaatt caattaaaag aaggcatcct gacttagttc
    12061 atttatctcc acacaaactc cgtcatactt atgcaactat agctagacaa ggtggagcag
    12121 atatgaatca aatttcaaat gctttaacac actcagatat ttctacaaca aagatatatg
    12181 tgaacacacc agatatagtt gataaggctg tttttgaagc ttttcagaga ggattgaata
    12241 agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat
    12301 aattgaaaag tgccagaaac gttgatatat aaaggatatt aacgttttga gaattgtgaa
    12361 gctttacgtg ctttcttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta
    12421 agtaagccag cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga
    12481 gcgataccat gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca
    12541 aatacgtcat atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc
    12601 aagtcagcat gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt
    12661 cctggtacta gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga
    12721 acttgtgcca atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt
    12781 tgttgtgctg catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg
    12841 cggcctaaag tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa
    12901 tttttagcac gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga
    12961 cggtaataaa ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg
    13021 acgatgacga aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt
    13081 agtacagatg caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca
    13141 taccatttac gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc
    13201 tccaatttaa gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca
    13261 aacaatacca tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga
    13321 ttgaaaaatt tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc
    13381 tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg
    13441 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa
    13501 gaagctgttt tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc
    13561 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
    13621 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
    13681 ctttttcggt caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat
    13741 gagcttggat catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt
    13801 gtgttggatc gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta
    13861 gataacgata gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc
    13921 caatttcatc agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac
    13981 cgtctaatgt gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga
    14041 actcatccat cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt
    14101 ggtgaacaac aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg
    14161 tttgttggaa tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag
    14221 actttcaatg aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt
    14281 tatccgtaaa gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg
    14341 ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata
    14401 ttttttatga tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc
    14461 ttcactgata ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc
    14521 ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg
    14581 acgaaaaatc ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc
    14641 ctggcaaact ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt
    14701 catcatttct ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc
    14761 gtcccaaagc ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc
    14821 cgctggcaca aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt
    14881 agcagattga acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc
    14941 attgctagtc gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc
    15001 catttctgga tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac
    15061 ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc
    15121 tgcccctccc aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga
    15181 tggtagacct gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat
    15241 gttcaagtag tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt
    15301 tcctgaagct gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc
    15361 tttgataccg acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca
    15421 cttgattttg ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc
    15481 tttgccaagc acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca
    15541 agtgatcatt cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc
    15601 agaggagaag gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt
    15661 tataacggtg cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt
    15721 ttcttggatc aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa
    15781 agcaactgct ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc
    15841 acctcaaatt ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa
    15901 agggggagat ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg
    15961 tttaaccgcc cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt
    16021 caaatgctgt cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca
    16081 acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc
    16141 cttatgtggt ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac
    16201 gttcatatgg atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt
    16261 ccattctcac acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg
    16321 cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta
    16381 tggtcaatcc tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt
    16441 gtctctagaa gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt
    16501 ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt
    16561 gagcagtgcg ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga
    16621 cttgtcacaa cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg
    16681 cgacaggttc acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct
    16741 caaaatgctg gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc
    16801 ttatttcttg acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata
    16861 aagattgtaa tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata
    16921 aaattcgctt cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg
    16981 caatgcttgt tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac
    17041 tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg
    17101 ctgccaatag atttccacca actgatcata agaaataact tctggatcat attcgatctc
    17161 tactgcttct gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac
    17221 atgcccgcca gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt
    17281 gaccatacac caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc
    17341 ttttttctca tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa
    17401 aacggaaact attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga
    17461 tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct
    17521 attcccaata catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca
    17581 atgaccaagc atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct
    17641 ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc
    17701 aaattcgttt tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg
    17761 atgtttgttt ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca
    17821 tccatttttt gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc
    17881 tctttttctg agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg
    17941 gttctggata ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg
    18001 ttagctaaaa ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct
    18061 aaatcgaata gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa
    18121 gtaaagatgc gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt
    18181 cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg
    18241 ctttttcgct tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta
    18301 ctcttctagc aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt
    18361 cgcctacgat tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata
    18421 cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc
    18481 caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat
    18541 catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga
    18601 aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga
    18661 ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta
    18721 gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac
    18781 cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt
    18841 gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc
    18901 taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa
    18961 aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag
    19021 taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt
    19081 ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac
    19141 ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg
    19201 caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta
    19261 ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg
    19321 tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt
    19381 ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc
    19441 tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc
    19501 atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata
    19561 cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga
    19621 tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga
    19681 aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag
    19741 ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt
    19801 gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg
    19861 ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa
    19921 aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt
    19981 cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat
    20041 ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat
    20101 gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg
    20161 aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg
    20221 tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg
    20281 tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc
    20341 gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata
    20401 taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc
    20461 gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg
    20521 taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt
    20581 tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt
    20641 ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag
    20701 agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc
    20761 tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt
    20821 agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc
    20881 gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg
    20941 tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac
    21001 tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac
    21061 tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc
    21121 tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat
    21181 ttttatttcc catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa
    21241 aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc
    21301 ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt
    21361 gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt
    21421 tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct
    21481 gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca
    21541 tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg
    21601 aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt
    21661 ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct
    21721 aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg
    21781 aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt
    21841 actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc
    21901 aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac
    21961 cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg
    22021 atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt
    22081 ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg
    22141 cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg
    22201 ggtgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta
    22261 tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca
    22321 accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt
    22381 tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc cctcctttgc
    22441 ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg
    22501 tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc
    22561 ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt agcgtttgaa
    22621 aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat
    22681 ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa
    22741 tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt
    22801 aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca
    22861 aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga
    22921 ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga
    22981 acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca
    23041 aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc
    23101 acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt
    23161 tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac
    23221 agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg
    23281 taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg
    23341 gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc
    23401 aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat
    23461 tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac
    23521 gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt
    23581 agcaaaggca acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa
    23641 tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc
    23701 ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt
    23761 ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc
    23821 ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc
    23881 atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta
    23941 acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac
    24001 accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg
    24061 atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg
    24121 aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca
    24181 ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt
    24241 tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg
    24301 gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt
    24361 ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa
    24421 taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg
    24481 ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta
    24541 aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa
    24601 ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc
    24661 gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg
    24721 gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca
    24781 tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata
    24841 atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt
    24901 tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta
    24961 ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg
    25021 gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa
    25081 tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca
    25141 aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc
    25201 tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc
    25261 aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa
    25321 ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc
    25381 tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt
    25441 gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc
    25501 tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata
    25561 cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc
    25621 gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat
    25681 tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac
    25741 ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa
    25801 aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac
    25861 tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa
    25921 tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat
    25981 attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag
    26041 tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg
    26101 ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt
    26161 cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg
    26221 gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt
    26281 ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag
    26341 ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt
    26401 attcactggt tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg
    26461 agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga
    26521 aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa
    26581 accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat
    26641 tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc
    26701 tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt
    26761 atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg
    26821 agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt
    26881 attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc
    26941 tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct
    27001 aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc
    27061 ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag
    27121 ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt
    27181 accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg
    27241 cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact
    27301 taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt
    27361 agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt
    27421 aacaataatt tttttcattt ttttccacct cttattttat tattttcagc cacatatatt
    27481 catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca
    27541 ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga
    27601 taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt
    27661 ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca
    27721 tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa
    27781 tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc
    27841 ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta
    27901 acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg
    27961 ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat
    28021 ctgggttttt cttgtagttt actcgggtac cctctttctt cagctgttcc actaattcaa
    28081 ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg
    28141 ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt
    28201 tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact
    28261 gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg
    28321 gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga
    28381 ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc
    28441 ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa
    28501 gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt
    28561 cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta
    28621 acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa
    28681 aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag
    28741 acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat
    28801 cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat
    28861 cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact
    28921 tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc
    28981 cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct
    29041 tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg
    29101 agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg
    29161 agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta
    29221 aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc
    29281 ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat
    29341 agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg
    29401 tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat
    29461 caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg
    29521 tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag
    29581 gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca
    29641 atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg
    29701 acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga
    29761 tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc
    29821 tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact
    29881 acacgaacat ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa
    29941 cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg
    30001 tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc
    30061 gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg
    30121 atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat
    30181 tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg
    30241 caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca
    30301 ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc
    30361 aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc
    30421 aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt
    30481 actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt
    30541 tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta
    30601 ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt
    30661 cgttttttta aacacattta cccttatttc aatccatgtt atttggggat acttatctct
    30721 aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca
    30781 ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat
    30841 tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg
    30901 ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta
    30961 gacaaattat tttagac
//
LOCUS       DACCZY010000021        30817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCZY010000021 DACCZY010000000
VERSION     DACCZY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.021"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            622..1950
                     /locus_tag="IVZ67_000762"
     CDS             622..1950
                     /locus_tag="IVZ67_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8164860.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2152..2985
                     /locus_tag="IVZ67_000763"
     CDS             2152..2985
                     /locus_tag="IVZ67_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8164861.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3001..3738
                     /locus_tag="IVZ67_000764"
     CDS             3001..3738
                     /locus_tag="IVZ67_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8164862.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3893..4861)
                     /locus_tag="IVZ67_000765"
     CDS             complement(3893..4861)
                     /locus_tag="IVZ67_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8164863.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5093..5926
                     /locus_tag="IVZ67_000766"
     CDS             5093..5926
                     /locus_tag="IVZ67_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8164864.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5960..6799
                     /locus_tag="IVZ67_000767"
     CDS             5960..6799
                     /locus_tag="IVZ67_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8164865.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6825..7385
                     /locus_tag="IVZ67_000768"
     CDS             6825..7385
                     /locus_tag="IVZ67_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8164866.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7584..9305
                     /locus_tag="IVZ67_000769"
     CDS             7584..9305
                     /locus_tag="IVZ67_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8164867.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9470..10612
                     /locus_tag="IVZ67_000770"
     CDS             9470..10612
                     /locus_tag="IVZ67_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8164868.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10628..11839
                     /gene="thiI"
                     /locus_tag="IVZ67_000771"
     CDS             10628..11839
                     /gene="thiI"
                     /locus_tag="IVZ67_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8164869.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12412..13059
                     /locus_tag="IVZ67_000772"
     CDS             12412..13059
                     /locus_tag="IVZ67_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8164870.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13452..16097
                     /locus_tag="IVZ67_000773"
     CDS             13452..16097
                     /locus_tag="IVZ67_000773"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8164871.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16407..17720
                     /locus_tag="IVZ67_000774"
     CDS             16407..17720
                     /locus_tag="IVZ67_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8164872.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17710..18363
                     /locus_tag="IVZ67_000775"
     CDS             17710..18363
                     /locus_tag="IVZ67_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8164873.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18418..19110
                     /locus_tag="IVZ67_000776"
     CDS             18418..19110
                     /locus_tag="IVZ67_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8164874.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19260..19460
                     /locus_tag="IVZ67_000777"
     CDS             19260..19460
                     /locus_tag="IVZ67_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8164875.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19714..21393)
                     /locus_tag="IVZ67_000778"
     CDS             complement(19714..21393)
                     /locus_tag="IVZ67_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8164876.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21395..21607)
                     /locus_tag="IVZ67_000779"
     CDS             complement(21395..21607)
                     /locus_tag="IVZ67_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8164877.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22000..23730
                     /gene="efrA"
                     /locus_tag="IVZ67_000780"
     CDS             22000..23730
                     /gene="efrA"
                     /locus_tag="IVZ67_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8164878.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23727..25487
                     /gene="efrB"
                     /locus_tag="IVZ67_000781"
     CDS             23727..25487
                     /gene="efrB"
                     /locus_tag="IVZ67_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8164879.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25582..25779
                     /locus_tag="IVZ67_000782"
     CDS             25582..25779
                     /locus_tag="IVZ67_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164880.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25932..26417
                     /locus_tag="IVZ67_000783"
     CDS             25932..26417
                     /locus_tag="IVZ67_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8164881.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26521..27114)
                     /gene="clpP"
                     /locus_tag="IVZ67_000784"
     CDS             complement(26521..27114)
                     /gene="clpP"
                     /locus_tag="IVZ67_000784"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8164882.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27294..27821
                     /locus_tag="IVZ67_000785"
     CDS             27294..27821
                     /locus_tag="IVZ67_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8164883.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27912..28649
                     /locus_tag="IVZ67_000786"
     CDS             27912..28649
                     /locus_tag="IVZ67_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8164884.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28653..29570
                     /gene="miaA"
                     /locus_tag="IVZ67_000787"
     CDS             28653..29570
                     /gene="miaA"
                     /locus_tag="IVZ67_000787"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8164885.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29572..30801
                     /gene="hflX"
                     /locus_tag="IVZ67_000788"
     CDS             29572..30801
                     /gene="hflX"
                     /locus_tag="IVZ67_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8164886.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10175 a         5272 c         6577 g         8793 t
ORIGIN      
        1 gtaggttcga gtcctactgc cggagtaaat aaagcttatg caaagaaaaa gaaatccaat
       61 cacttgggtt tctttttctt ttattattat gatgaatcaa gctttctgtt tttgtcacgt
      121 atgttgtaat ctttacgaaa gagtagagtt ggctactcat aatcaaccat acttcccatc
      181 atcgctaaat acgaaataaa tcagcattcc tacaccaatc aaagcacaag ctgcaccaag
      241 aaaagggatg acattcccaa agttatctgt attcataagt atgccaccac ccacaaacaa
      301 aaagccgcca gcaatcaggt ttttcatttt attcatctcc tttcttacgt tatagtattt
      361 ccttctttca atgagtattt tgaaagctat cttattgtat gatgttcttc taggcctctt
      421 ctttattata caggccattt tcgttatctc atagaaatat tcctttttat ctgaaaacta
      481 ttgtataccg tatgacattt ttcttcatga agttgtaagt atattctcat tcaaatgaaa
      541 atttcatcat agaagagatt gtatttctaa attcttaaga gtaaaatacg aagtataact
      601 tggatttgga aggatgattg gatgaaagct attacgcctg atatgacaac aaaatttaac
      661 aaacagtttc aagcaaatat caaacagcag gcattgcagc gggcagttgt gaagaacgga
      721 attactgctt ctgctgaaaa tgtttcagca catgtggaga atacaccggt attttctata
      781 gatttggcta cggggaaagt ggctaatcaa atgcaatcgg ggcgttgctg gatgtttgct
      841 gcactgaaca cattccgtca taagatgctg aatcaattcc atttaaaaga gtttgaatta
      901 tcacaaaatt acactttttt ctgggataaa tatgaaaagg caaattattt ctatgaaaac
      961 atcattgcaa caggaaaaga ggcaatcgat agccggaaag tagctttctt actcgctact
     1021 cctcaacaag atggcggcca atgggatatg atcgtttcat tattccaaaa atatggcgtt
     1081 gtgcctaaat cggtgatgcc ggaaagtgcg aatagttcaa attctcgaga tttgaataac
     1141 tacttgaaca aattactgcg taaagatgca gtagtgttac ggaaaatggt tgctcaagga
     1201 gaaacattag acgctattga agaaaagaaa gaagaaatgc tagaaagcat ttataacttc
     1261 ttagctatct cattaggaac acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa
     1321 aagaactatc atcttgaccg aggactgaca ccacaaattt tttatgataa atacatcggt
     1381 gtaaaattag atgattatgt tagcgtgatc aatgcaccaa caaaagacaa accattcaat
     1441 cgttcttata cagtagagat gctaggaaat gtcgttggcg ggaaagaagt caaatatctg
     1501 aatgttgata tggaaacttt caaaaaatta gcgattgctc agcttgaaga aggagaatct
     1561 gtttggtttg gttgtgatgt aggtcaatct tctactagaa ctagtggcat catggcctta
     1621 gatgcttatt caatggatga tctatttgat acggatttta caatgactaa agctgaacgt
     1681 ttagattttg gagaaagtct gatgacccat gcgatggtat tgaccggtgt tgacttagta
     1741 gacgggcaat caacaaaatg gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat
     1801 ggcttctttg ttatgagtga tgcctggatg gacgaatata cgtaccaaat cgttgtaaga
     1861 aaagatttat tgacgaaaga gcaattaaat gcgttcaatg aagaaccaat tgttctatct
     1921 ccgtgggatc caatgggtgc tttagcatag acgagtattt caatgaagag tatgtagtaa
     1981 agataaaaag agctggctta agccagctct ttttatcttt aaagtccatt ccctgttttt
     2041 tatggtccct gtgctataat tctaaataaa ggataaggat tggaaataat ccttttatca
     2101 ttaaaaatat gatgacgaag tagaataaaa ttgggagtag gggggaaagt catggatatc
     2161 caaactgttt tatctgatct gaaattaaat ggtaaagtga ttccagttgt tggtggcgat
     2221 gttaatcaga cctatcgtat aaaaaccgaa catcgtgctt attttttaaa aatccatccg
     2281 aatgtaaaaa aaggattttt tgaagcagaa gtagacggat taaaagaact gtcagcattc
     2341 gtacgtgtac cggatacgta tatgctaggt gaaacaagtg aaggcgctta tttgttaatg
     2401 gaatggatcg agccgggaaa aggggatcaa cgagatcttg ctgcagcttt agctaatctc
     2461 catcagcaaa cagcgccaca atttggtttt cgtaaagata actatttggg tacacttgtt
     2521 cagaaaaatt cttttgaaga agattggtgg acttttttct ttaaagatcg gcttgaatcc
     2581 cagatttctt tggcagaaga aacgaatcga tggaatgtac agcgtcaaga aaagtatctg
     2641 cgatttaaag aacgtgtatt gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg
     2701 cacggagacc tgtggagtgg taatgtgttc tttgaccagc aagggcatcc agtttttgtc
     2761 gacccagctg tttcttatgg gaatcgggaa caagatattg caatgagcca gttattcggt
     2821 ggtttccgac cggaattttt agatgcttat caaactatat ttccattaga aaagggatgg
     2881 aaagaccgac tgccgattta ccagttatat tatcttttgg cacatttgaa tatgtttgga
     2941 gaaagttacg gttcccaagt agatcaatta ctagaaaatt tttaagagga gggcgtgttt
     3001 ttggcaaaaa aatataccaa aaagcatgaa gtttcatatt atgagtgtga catcaaccaa
     3061 acgatgacat ttccttcgat gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac
     3121 cagttgggaa gaggcagtga attcgtcaat agctttggac ttacttgggt catcacgaat
     3181 tattccatgg agattaatcg tctccctaga gtcggtgaag taatctcagt aacgactcaa
     3241 gccatggagt acaataagtt tttctgttat cggaacttct ggatccatga tgaagatggg
     3301 aaagaattag tcaaaataga atctgttttc gtattgatgg attttgtgaa tcgaaaaatg
     3361 agcagtgtta atgaagagat cattgctcct tttgagagtg agaagatcaa gaaaatcaag
     3421 cgtcaggaaa aaattgaaaa gattgattcg ggcttcatgt tgccatatcg tgttcgtttt
     3481 tatgatatcg atagtaatca gcatgttaat aatgcgatgt attttaattg gatcattgac
     3541 gtattaggat atgacttttt gactacgcat gttccagaaa aagtaattat tcgatttgat
     3601 aaagaagtag agtacgggaa tgagatcgaa agccattttg aacaaatccc tatcgaaaat
     3661 ggtgtcaaaa caaaacatga aatccgaatg caagaacagc tgtattgtga agcaaatatt
     3721 atatggaaaa ataaataaaa aagaggttgt gacagaaggg ggcaacttca agaaataaga
     3781 agaaatttac gaaaattgtt tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa
     3841 gctatttttg tcacagcctc tctttttaag tttagctatt agataatgat gtttagtttt
     3901 ggataaacga aatcaattct tcacccgaca aaccatagtt tacagcaatc aatagtttta
     3961 aacgtgcttt ttgactatta atactgttac agaaaataat ccccattttt tctagttctt
     4021 ttcccccgcc ttcataatca taaacaggct ctgcgattcc attgaaacaa cgagaaacaa
     4081 gtactacagg gattttttgg acaagaagct tttccaaagc agctaaggct tgtggaggga
     4141 gattccccgc acctaaagct tcgattacca aaccgtctac tttcgtatgg gcgatggctt
     4201 ccaataagtc cccttgcatc ccagcatatg ctttaacgat cgggatagtc ccatctacag
     4261 cggaaatgtc cagtcgcttt gtttctaaaa gttcctgcaa gaataaaatt cgattcttag
     4321 ttactaagcc gatgggaccc aatgtaggtg ttcggaaagt agcaacattt gtggtatggg
     4381 tctttgtcac gtagcgtgca gaatggatct catcgttcat gaccaccaaa acccccttat
     4441 ctagcgcttc ttcacaactt gcgacacgaa tcgcactttc aaaattataa aggccgtcac
     4501 ttccaagttc attacttgaa cgcattgccc cagtcaaaac aattggtaat ttgttgccaa
     4561 tcgttgtatc aagaaaaaaa gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca
     4621 ctccggatat acctgatgca taagcggaca agatacgttc ttttaatacc aacatgtgag
     4681 aaggcatgat gtgtggtgag ggaagattga agatatcttc aactaataat tctacatgct
     4741 tggggattgt gatttctgca tctaataatg ggtttgccac atcaggacta acaccgcccg
     4801 tgagcatgtt ttctttcatg gcgatcgttc cgccggtgtg tagtacaagt atttttttca
     4861 ttttacagac ttccttacga ataattttgc atagccgtta aatcttattg tatactatat
     4921 aaaagagttt tgctatcagt gaagaataac gaatagccta acttatcata aaaaagctga
     4981 ccttattaaa atggaaaaga ctcaaccttg acctcctaat acaatggagt atctggttga
     5041 gtttctttta caagaaagca gccaaggaaa tcaatcacga aggtggccgg aaatgatcag
     5101 aatcggatta acatcgttta aagaacatga caaattaact ggtaaaaaga ataacactct
     5161 ttatgaatat gcagggtttc tacccttagt ggaattagat actgcctatt atggaattcc
     5221 ccgaaagtcc tcagttgaga attggataag tgaagtacct gaatcatttc gctttgtagt
     5281 caaagtctat caaggaatca gcggtcaagg agactggaag ccttattatc agaccgaaga
     5341 agagatgatt gcgcattttt tagatgttat gtcgccaatg gtagatgctg gaaagatgtt
     5401 ttgcttttta gtccaattcc cctcatcatt caaatgtacg aaagaaaatg tccagtactt
     5461 gcgcaaactt agaaagtggt ttgagggtta tcctgtttct atagaattgc gggattattc
     5521 ttggtacagc gatgaatttc ttgaacgtac aagaaacttt atgtctagag ccgctttttc
     5581 tttagccatc atcgatgagc cacagttgct gaatacaacg attccttttg atccatttgt
     5641 caccaatccg aactttgctc tttttcgatt ccatggtagg aatgcgcaag gatggcaagc
     5701 aaatgataaa gagtggcgta aaaaaagaac gttatatcgt tattcagaaa aagaactgca
     5761 agaactagca catgaagtca aagtagttga ttctaagaca aaagaggtag gtgtgatttt
     5821 taataataat tctggtggtg atgcggctga aaatgcatta gcactgcaaa aaatattaaa
     5881 tatcaagcca gatggactca atccaaccca aatggatctg ttttaagggc ttattttatt
     5941 caagaaaggg gcgaagtcta tgatcaaagc tatttttttt gatattgacg gaacattggt
     6001 caccaatcgt tcaaaagcgt tggaatcaac aaaacaagca attgaatatg ctcggaaaaa
     6061 cggcatttta tgcggtgtag caacggggcg cagcccagta aagatcaaag aaatcattga
     6121 tgaattggaa ttagatatgt atgtcgtgta taatggtcaa ctggttttta ctgcagatcg
     6181 cacgattatc gaccaccctt ttgaacaaaa agtattggaa catattgttg aatttgcaga
     6241 tgagaaccat cgacaaatcg tttttggtgc gagaaatcgg ttagatggat caacgacgat
     6301 gctattagga caatcgattt ttatcaagcg actagtcagc tttttaccgc gtaaattccc
     6361 ggtacgtctg atgaaaaaaa tcctccaggt ttttagccct caccgacaaa aggatcgtta
     6421 tgaaacgtta gagatattaa aagaaccaat ttatcaatgt atattgctca gtccggagtc
     6481 agaacagaaa aagttagaac atcgctttcc cgagtgtacg tttcagcgtt caaatacgta
     6541 tactgtagat atcatcccaa aaggtggttc aaaattacta ggcatccaag ctttcgcaaa
     6601 tgctgtcgaa attgaaatgg aagaaattat ggcattcggc gatcattaca atgatatcga
     6661 gatgctgaaa ggtgtaggaa ttggagtggc catggggaat gctcagatcg aagtaaaaca
     6721 tgctgcagac tttgtgactc aatcaaatga agaagatggg atatacgaag gactgaaaca
     6781 cttcggtctg atttattagt caatgaaaga aggaaaaaat cagtatggaa gcagagacac
     6841 cttttcaaaa agtagaaaca ttccacaaaa cttttgatcc aagaagacca gagataccca
     6901 cggcttttac agaaaatgaa gctcatacac gtgctgcgtt caaaatagaa gaacttgttg
     6961 aatttgttgc agctacttca cctaataaag aacaactgca tgaagctgtt atgctgctgc
     7021 acgaagcatt agataaagca gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc
     7081 cgctggttga acaagtagat gcactgacag atatccttta ctttacttat ggttcttttt
     7141 cattactagg aatagtgcct gatcgaatat tcgaaatcgt ccatcaggca aatatgggga
     7201 aattatttcc agatggaaaa cctcattatg accctgtaac aaataaagtg ttgaaacctg
     7261 aaaactggca gatggaatat gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga
     7321 ttcaagaagc gattgctaag aagaaatccg ctgatatagg catgtgcaca aatgaaaaag
     7381 agtgatcaga gtttcactat ttttgttatc tttatacgat ggaaaaacaa aagattttcg
     7441 tttaagttag tgaatgcatg aaaatagtat gctataatct agaatagatg tcttttgcac
     7501 gagagacgta ttatttggat ttatgccaag cagaacgcca tctgttgcat aaaagatgca
     7561 tactgaaaac ggaggaacca taaatgggaa ataatgttat catcggaatc gtcattgcaa
     7621 tcattatagt cgccattgtc gtctttttgg ttagttactt gatgaagaaa aaaaaccaag
     7681 atcgattgga tgccttagaa aaacgcaagg aagaattatt tgacttgcca gtgattgaag
     7741 aagtcgatga tgttaaaaaa atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat
     7801 ggagtcaaaa atggaatgac atatctactt cctcgttcgc tgaactagaa agtcaaattt
     7861 tcgaagtgga aaacctcaat gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg
     7921 aagcagaagc tacaatggac caaatggaga aacaagtgac ggaaatccgt gaaggtctaa
     7981 aggaattgcg ggaaacggaa gaacgcaact cactagctgt tcagcaggca cttgacgtct
     8041 atgaagaact gaaagaaaaa gtcagagaag acgagcatga atacggacca gctctttttg
     8101 aattgcaaaa acagatcaaa gtgatcgaaa aagaatttac gcagtttgtg actttaaaca
     8161 cttctggaga ccctgttgaa gcccgcgagg ttttggataa agcagaaaaa catacgtatg
     8221 aagtggaagc tctgatgaaa aaaattccac cgctgtatga ggagctccat accactttcc
     8281 ctgaacaact tgaagaaatc agtgatactt acgagaaatt gatggaagaa caatatgtat
     8341 tcccagaaga agatcttgct gaagatatcg caaaagtaag tcgaagaatt gaaaattcat
     8401 tagccaatct tgaaaaaaca gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg
     8461 atttgattga ttcgttatat gatatcttag aacgggaaat ggaagctcag agatatgtaa
     8521 aaactaacca atcaacgatt gcagagtata tcaaacatac aacaaagaat aaccgtcaat
     8581 tattgattga gctggatcac acatctcaaa gctatacatt gaaccacaat gaaattggcc
     8641 gtgtacgagg tttccaaacc gaagtggaag aaatggaacg tcaaaatgaa cagatgattc
     8701 cacagatccg tcaacacgaa attccttatt cagaagtgcg cacgttctat aaaacagtct
     8761 tcaaagtctt agaagatatc gaaaagcaac aagtagagat cgacgactca ttacatgaat
     8821 tgagaaaagg cgaaaaagaa gcgcaagaaa agatcgatac attcgaattc aaattgcgca
     8881 gtttgaaacg ttttgtagaa aaacaacgtt tgcctggctt gccgaatgat tacctagaat
     8941 tcttttttgt tgctactgac cgtattgaag aactaagtgt tgtcttgaat aaaattcgag
     9001 taaacatgga agaggtcaat cgtttagttg ctctatgtga agaagatcta gaattattag
     9061 ataagaagac acatgattta gtagatgcag cagcattaac ggagcaaatg ctccaatatg
     9121 caaatcgtta tcgtcataca cacgaagaag tgagagaagc aattgataaa agctactatt
     9181 tattcaataa agaatatcac tatcaagaag cattagacga aatcggtaca gcattagagc
     9241 gagtagaacc tggtgctttc aaacgcatcg aagattttta ttttaatcat ccagacttag
     9301 tataaagcaa aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt
     9361 ctttttttgt atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc
     9421 ttggacaacc gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt
     9481 tgataacagt gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag
     9541 tcagcggatt attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt
     9601 gttgcagcaa gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta
     9661 ttttacttca ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa
     9721 aagagaatat gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac
     9781 agcagaacaa ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg
     9841 atttgtacgt gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt
     9901 tatggctgtg aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt
     9961 ggatgatttt cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc
    10021 attctcgcaa tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca
    10081 tggcccacgg ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact
    10141 gacaggtgga gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt
    10201 ggctatggtt aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac
    10261 acaacgctta aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt
    10321 ttcagaagga actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg
    10381 cgaagttctc gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg
    10441 ctcctcacgt aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct
    10501 ggctacatct gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca
    10561 atttatgatt attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag
    10621 tgttcttgtg aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa
    10681 gaatcgaaaa agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt
    10741 tccagaaatc aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga
    10801 cgggcagcta gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag
    10861 tattcgtgta gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga
    10921 aatttatact gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga
    10981 attgacaagt aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca
    11041 tatccaagtt caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg
    11101 cgcttatcta tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg
    11161 aaaaggaatg cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat
    11221 gaagcgggga gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca
    11281 agcactgcaa aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca
    11341 ttttatcgaa gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg
    11401 atattggatg acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat
    11461 tcgtcatggt ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt
    11521 gcaaagtatg gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac
    11581 aatggataaa cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat
    11641 ccagcctttt gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca
    11701 actggaaaag gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc
    11761 actagctggc ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga
    11821 attttcagaa ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa
    11881 caaaacgaaa aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct
    11941 gattgaatac catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa
    12001 ataaaatttt atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc
    12061 catgaaatag cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg
    12121 aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg
    12181 aacaccgtgt atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa
    12241 acaaaaatag ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt
    12301 tgaacaccgt tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg
    12361 ttagaaacat gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa
    12421 aaaacagata cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg
    12481 aagttgctgg atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc
    12541 caaattcctt ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc
    12601 ggatatggct atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa
    12661 gaagaaaaac gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac
    12721 aattttagaa gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg
    12781 atcaatcgag taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc
    12841 aaagcaaaaa atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct
    12901 attgatgaaa ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta
    12961 actgtcccag cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt
    13021 atttactttg atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt
    13081 gatcttgcga gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt
    13141 ttttcacgac cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga
    13201 acaagaggag taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc
    13261 aatgacaacc agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga
    13321 gtcgtgatcg ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta
    13381 ccgcgtaaat catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg
    13441 aggaaaacag catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg
    13501 aagctggacg ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa
    13561 aagcaaaacc ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag
    13621 ggcatgcttg ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat
    13681 atgatacttt atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag
    13741 aagcgaagct tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg
    13801 atcaagtgtg ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa
    13861 tgggcttatc tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag
    13921 cagtacgtaa agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata
    13981 tcatcaactg ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag
    14041 atatcgaagg cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag
    14101 aaattgcgac aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag
    14161 aagacgagcg ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac
    14221 aaatcccaat cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa
    14281 tcactccagc acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg
    14341 taaatgttat ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg
    14401 aacgttttgc tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta
    14461 aaatcgatac gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac
    14521 ctcggctgtc tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata
    14581 accaagaaac agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac
    14641 gttggatgga aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa
    14701 ttcctgcatg gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg
    14761 atagtgaaaa ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt
    14821 ggcctttctc aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc
    14881 ctactagcac tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt
    14941 tccaaagctt ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga
    15001 ttcgtgacga acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg
    15061 aagtaatcga aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac
    15121 caggacaaga tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca
    15181 aaatctggaa tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga
    15241 tcgatttatc tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa
    15301 cgatcgaacg agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac
    15361 tgtataactt tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac
    15421 tttatggaga tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg
    15481 atcagatcct acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac
    15541 atattcctca tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt
    15601 ttgatgatga aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc
    15661 gaaatattcg ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa
    15721 caaacgatgc taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct
    15781 gtaatcccga agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag
    15841 ctgttctgac aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag
    15901 aaatcaaacg attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag
    15961 gaaaactttc taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc
    16021 gtgcaaaaga aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt
    16081 tgcgtactat tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa
    16141 gctagctgat tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca
    16201 taaggagatg tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg
    16261 agaaggaggg aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct
    16321 ctctctgttt cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata
    16381 ataaaagaga aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata
    16441 gtcgattacc atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg
    16501 tagggcatcc agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt
    16561 ctactgtagc ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta
    16621 cttcacctta tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag
    16681 acaatgattt gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat
    16741 ttgaagcagt agctggtatt actgaattcg agacattaac agcaatggca tttgattatt
    16801 ttttgatgaa gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt
    16861 ctaccaatgt agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg
    16921 aaatacttgg agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag
    16981 gcgttccagt agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag
    17041 ctaaagcaaa aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc
    17101 atcctgatga gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc
    17161 aattaaccac tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat
    17221 tgtattttgt ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg
    17281 gattgaagaa atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg
    17341 ttttagatgg tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag
    17401 aattcaaagg atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag
    17461 aaatgctaga acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac
    17521 atccaaaagc catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg
    17581 tttcattgtg gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat
    17641 tagttgtgac cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag
    17701 gaggagatca tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca
    17761 gagttagtgt attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat
    17821 aaagacctgt atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat
    17881 atgattttct cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag
    17941 gaaacgcttg aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc
    18001 ctctcttttt tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca
    18061 gtcattgaac ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct
    18121 gcagaaaatg ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag
    18181 ctaggtacag cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca
    18241 gcttctgcgg ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa
    18301 ttgatcaaaa aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa
    18361 taaaaatcca tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg
    18421 ggaaaacgat tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt
    18481 tatggcgcgg aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa
    18541 cacccttata gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc
    18601 tctttacgac aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa
    18661 gcaatcgaaa tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga
    18721 gcggctccac cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa
    18781 gatcacaaac aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta
    18841 aaaaaaacag tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc
    18901 cattatgctg tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt
    18961 aatgttattc catcgcagca ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa
    19021 atgatgggga tcactgtact ggaccacttg atcatcggac gcaaacgata ttttagtttg
    19081 agagaagaag gaatgatgga agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag
    19141 tgtacaatta tattgtaatt ttgtttggga atagaagttg aaacatctgt tgcacctcta
    19201 tcgtacacga gcaatgttac aatttaaatt agtgcttttg gcacgaggag gatattatta
    19261 tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg atttggattt atctcacgtg
    19321 aagatggaag cgacgtattc gttcacttct cagctatcca aggtgacgga ttcaaaacat
    19381 tagaagaagg tcaagcagtg actttcgacg ttgaagaatc agaccgcggc ccacaagctg
    19441 caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt gatggtagac atcaattttt
    19501 acgagagaac aaagcgagta acaaaaatac ctgccacaag agacaatttc tcttgtggca
    19561 ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc tatgaaagag ccaccttgta
    19621 tcaaggaata agaacaaaca aaaatggctt ctcatgtttt tcatttgttt tctcttatat
    19681 tcaggacaag gtgctgattt cataacctct tatttattct tcatctggtg tcattaccat
    19741 tggcaaaatc attggatgtc tttctgtacg ttcgaataag aaaggctgaa gcgcgccagt
    19801 catcgcttct ttcaatttat attctgaaca gtcaggtcca ttcaaacatt ctcttaaagc
    19861 gtggaataag attcgctgag cttcattgat catttctcct gattctcgca tgtagacaaa
    19921 tccacgagag agaatatctg gaccagccaa gatttctttt ttcttgatat ccactgtcgc
    19981 gacagctaat acaagacctt cttctgaaag caggtgacgg tcacgtaaaa cgacattacc
    20041 aatatcaccg atcccgctac catcgatata cacatctcca gccacaaaat gcccagcagg
    20101 acgcgcagaa tcgctagtta acgctaaaac atcgccattt tccatgatga agcagttttc
    20161 tgcaggaaca cctacatctt gtgctagaga agcatgtact ttcaacatgc ggaattctcc
    20221 gtggacagga ataaaatatt ttggcttcat caaacgaagc attaattttt gttcttcttg
    20281 tccaccgtga ccggaagtat ggatgttatt gatttttccg tgaatcactt ctgcaccagc
    20341 ttcagacagc aaattgatca aatgattgac acttgttgta ttaccaggaa taggtgaact
    20401 tgaaaagatc actgtatcgt ctggctggat ttggatttga cggtgagtac cattcgcgat
    20461 gcggctcaac gcggccatcg cttctccttg agaacctgta cataggatca tcgtttcatt
    20521 ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg ccgtctggaa ctttgatata
    20581 tcctaaacgt tgaccgttga cgatggcatt ttccatgctt cgtccgaaaa cagcgatttt
    20641 acgaccagtt gccacagcgg catctgctgc ttgctgcaaa cggaagatgt tagaagcaaa
    20701 gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc ttcaaaatag aatgcccaat
    20761 cgttttttct gatttagtaa atgttggaat ctcagcattt gtactatcag ataaaaggca
    20821 caagactcct tcttccccga tacgagccat tttatgcaag tttgctggtt cgccaacagg
    20881 agtgaaatcg aatttgaagt ctccagtcgc tacgatattt ccagaaggtg ttttcaccac
    20941 gatccctaat gcatctggga tactatgggt cgttctgaaa aagctgacag aagtctttct
    21001 aaaacggatg atcgtatctt cattgatctc atggagctct gcatctcgaa gcaagccatg
    21061 ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga ccagcataga taggaatatt
    21121 tgcttgtttc agcaagtagg gtattccgcc gatgtggtct tcgtgcccgt gagtaatcac
    21181 caaggctttt actttgtgta agttttgtat gatgtagctg tagtcgggaa tgacatagtc
    21241 aattccaagt aaatcgtctt ccgggaattt gatcccggca tcaatcaaaa tgatttcatc
    21301 ttggaattgt accccatacg tatttttccc gatttcacct aatccaccaa tagcaaagac
    21361 gcctgtctcg ttatttttga tggaaagttt catattaaaa ctccactaat gagaaatcag
    21421 cattttcttt ttcgtactct agatgacttc cttctagaag ttgaacgaat tcaatattgt
    21481 atggggtatt ttcttccaca cgttggcgag cgtctacatc gctttttgcc tcaatgtaaa
    21541 gagattttgt ttcttcccgt ttagggttgc gtacttttgt ttcttgataa taaactttat
    21601 agatcattgt tttgtctcct ttatctgcgt aaagcagtga tttagctagt tgattttttt
    21661 agacaacaca aaacgagggg acaagccacc tggtttttca tatgctttga ctagaatact
    21721 ggattcgagt caaagagaca agcctcgttt ttgcttatct gttcggatat tttttcaacc
    21781 gtaccttctg tataaaaaat atacaacaat taattgttat tctagcataa aacagcaaat
    21841 aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt ttatcatccc gctataatca
    21901 taatgacttt tttatgagag aagtgttcta ttaataggct cgttgtgcta tcatttttag
    21961 taatgaaaat gtaacaaaag attaaaagga gatgagaaag tgaaactaat gtggcattat
    22021 accatgcgtt ataagaaatt cttgtttttc aatttcatat gcgtatttgg ttttattttg
    22081 attgaattag gacttcctac gattttagca cgtatgatcg atgtaggaat ccgcaatgat
    22141 gattttgatt atgtgaagca acaagggata tttatgatcg ttattactgt aatcggtatt
    22201 attttgaata tcttgttagg ttattttggt tcgaggatta cgactaatat cgtagcagat
    22261 attcgagatg atttgttcga aaagatccaa agttattcgc atcaggaata cgaaaatcta
    22321 ggcgtatctt cattgatcac tcgtacaaca aacgatgctt atcagatcat gttgttcctg
    22381 caaaatattt tacgtatcgg ttttatggca ccaatgatgt tcattgtcag tttgtacatg
    22441 gtcatgcgta caagtgtgac actcagtttg tatgtagtag gtgcattacc tttactatta
    22501 ttggcagtcg tagcgattgc taaggtgtct gaaccgttat ctaaaaagca acaaaaaaac
    22561 ttagataaaa tcaacagtat cttgcgggaa aatctttcag gattgcgtgt gattcgtgca
    22621 ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag tcaatgagaa ttatgcttct
    22681 agttcaaaaa gcttattccg tttgatggct gttgcacaac cgggttttta tttcttattt
    22741 aatatcgtaa tggtattgat catctggagc ggggcagtgc aaatcgatgc aggcgagctt
    22801 cgtgtaggag atttgattgc gtttatcgag tatatattcc atgccttgtt ctcattcatg
    22861 ttatttgcta gtgtgttcat gatgtatcca agagcagctg tttcagctgg acgtatccaa
    22921 gaagccctag atgccaaacc ttcgattgta gaagatccaa atggtatatc tgatacaaaa
    22981 actaaagggt atatcgaatt tcaaaatgta acatttgctt atccaggcca ttcgcaagag
    23041 ccagtgatac gaaatgtcag ttttacagcc tctccaggag agaccgttgc ttttattgga
    23101 agtaccggta gcggaaaatc tacgttgatc caattgatcc ctcgctttta tgatgtgagt
    23161 gaaggaaaga tcttgatcga tggagtagac gtgagagatt atcggctaag caagttgcga
    23221 gataagattg ggtttattcc gcaaaaagcg ctgctattta ctggaacgat tgcagataat
    23281 ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg aacgtgcagc tgagattgct
    23341 caagcttcag aattcatttc tcgaaaacca gatggattta atgaacattt atcagaaggt
    23401 ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa tagccagagc catcatacgc
    23461 cgtccggaaa tctatatttt tgatgacagc ttttctgcat tagattatca aacggatgcg
    23521 aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta ctgtattgat cgtagcacaa
    23581 cgtgtgggaa cgatcatgca tgcagataag atcatcgtat taaatgaagg agaagtcgtc
    23641 ggtatgggga cgcatcggga gttgttggaa aaatgcccga tttattatga tattgccgct
    23701 tctcaattat caaaggagga attagcatga aaaaaacctt ttcttcctta aaacgtttag
    23761 ctcgatatat cagaccgtat cgtgagactt ttcttttagt gatcctattt acagttctga
    23821 cagtggcttt caacgtggct ttgccatatg ttacaggact tcctactaca gaaatcagcc
    23881 gtaatattgc caatggcgaa tcaatcaatt tttcttatat attcaaatgt ctcatttgga
    23941 tcacagttgt tggaataggg tattgtattt cgcaattact ttctggtgtt ttgatgacaa
    24001 atgtcgttca aagtgccatg catgatttga gaagagacat agaagaaaaa atcaatcgtc
    24061 ttccggtttc atatttcgat aaaaaccagc aaggaaatat tttgtctcgt gtgacgaatg
    24121 atgtagatgc agtcagcggt gctttacaac aagcttttat tggtatcgta aatgcgatat
    24181 tgggaattac gttggctgca gcaatgatgt tttatatcca gccgatgatg gcgctgatct
    24241 ctatgatcat gattccaaca tcgatctgga tctcaaaacg agtgatcaat gcttcgcaga
    24301 aatattttca aagcatgcag aattctcttg gtgagctaaa cggctacgtc caagaaaata
    24361 tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa agaaacattg gaaggattca
    24421 aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc agcattcatt tctggcttga
    24481 tgatgcctct agttcaatta actgcctatg ctacttatat tggtatggct gttctaggaa
    24541 gcttttatgt tattactggg gcgatcgttg tcggtcaact acaagcattc atccaatata
    24601 tttggcaaat cagtcagcca atggggaacg tgacacaatt gtcttcagtt ttgcaaagtg
    24661 catcagcggc aacgaaacgt gtattcgaga ttttagatga gccagaagaa aaagaaaaca
    24721 aagtagatgc ttcactgcca gaaacgattt taggagatgt gacattcgat catgtcgatt
    24781 ttgcatatga tccgaagaaa ccattaatcc aagatttgaa ttttgaagtg aaagctggtc
    24841 aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac gacattgatc aatctattga
    24901 tgcgttttta tgacgtagac aaaggagcaa taaggattga tggaatcgac accaaatcaa
    24961 tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt gcaagatgca tggctttatc
    25021 aaggaacgat tgcagataat atccgcttcg gaaagcttga tgcgacagat tatgaagtag
    25081 ttgatgctgc gaaaacagca aacgtagatc attttatccg aacgatgcca gatggatatg
    25141 atatggccat caattctgag ggagacaatg tgtctttagg acaaaaacag ttgctgacga
    25201 ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt agatgaagca acaagttcag
    25261 tagacacccg tttagaagcg ttgattcaaa aagcaatgga tcgcgtgatg gagggacgga
    25321 caagtttcgt catcgctcac cgtttatcga caatccgtga tgcagatctg attctagtga
    25381 tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag tttattggca caaggtggat
    25441 tttatgaaaa actgtataac agccaatttg ctgaggaagc agaataagtc atataagaat
    25501 aaaacagttt aaaacttatt ttttgttctc tattcttgat aatggtaaag taaagttatc
    25561 aagaaaagtg aggacacgga gatggaacag tttttaaatg aaaaaattac tgaagccaaa
    25621 agagaagttg atcgtttaac gcacgtctgt cagacgaagc cttttgattc agctgatttt
    25681 tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa tagaagtgta cgaattaatt
    25741 ttaaaaaaag taatagaaga aaatatagat gtacaataaa aacaggtaaa acgtcagtta
    25801 ccagatactg taaatagaca tctggcctga gcaggtttta cctgtttttt ttcttatgaa
    25861 ataaatatat gctatgatac ctttattcaa taggattagt ttctacaaaa aatgaaagga
    25921 gaaaaataaa tatggaacgg tttatagcat caaatgccac agtgattggt gatgtgacat
    25981 tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg cggggacagt aattggataa
    26041 aaataggaca aagaacgaat atccaagatg gtacaatcat ccatgtcgat catgatgcac
    26101 ctgtagatat tgcggagaat gtgacggtag gacatcagtg catgctacat ggctgtacaa
    26161 tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt gaatcatgcg gttattggtg
    26221 agaatagttt gattggtgct ggatcattag taacagaagg aaaagttatt cctcctaatg
    26281 tcttggcttt tggtcgccca gctaaagtga tccgtccttt gacaaaagaa gaaatacaaa
    26341 aaaacaaaga aaacatccag cattatgtcg agataggtcg gatgcatgcg caaggaaaat
    26401 tcaaggagca aagataggca gtagcatcca taaaataaaa aaggatggtc agtttgagtt
    26461 gaccatcctt ttttatttgg tttagataag agagcttaaa aaagcttcct ttgaaacaga
    26521 ttaatttaat gaagaactgt tttccatgac ttcatcgatc aatccatatg cttttgcttg
    26581 ttcagctgtc atatagttgt cacgatctgt atctttttca atcacttcaa gcggttgtcc
    26641 agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt tgcaaaatat ggcgtgccgc
    26701 aatttcgatt tcagttgctt gcccttgagc gccgccaaga ggttgatgga tcatgatctc
    26761 agcatttggt aaagcaaatc gtttaccttt tgtaccagct gtcagcaaga agcttcccat
    26821 tgaagcagcc atacccataa caattgtttg gacatctgct ttgacaaagt tcattgtgtc
    26881 gtaaatagcc atacctgctg taactgaacc accaggagaa ttgatataaa gatagatatc
    26941 tttttcagaa tcttgagcgt ctaagaaaag caattgggcg atgatcgaat tagcaagatc
    27001 atctgttact tggccgctta acatgatgat acggtctttc aataaacgtg agtagatatc
    27061 gtaagctctt tcacctcgtg aagattgttc aatgactgta ggaattaaat tcataataga
    27121 gttcctcctt gatttcgttt agaaatagtg taccataatt tagtttctaa tacctaaaat
    27181 gtagtataca tttttggtca aaaaaggtca aataaaaaac ttaggcaaat tagtcgcata
    27241 aacccgcttt atggtatgat aagcagagaa acaaaacaag gaggaacaac agattgatag
    27301 ttggtattac tggccttatc ggagtgctga caggtgctgt agttgtctat atcggcatga
    27361 tactgcaaat gcgtcacttg gaaaataaag aacagcgtag acgtgaattg gaattgaaag
    27421 tcaaagaaat cgagacgctg aatcaattaa ataaaaagat caacgagatc ttgcaaaaac
    27481 ggacaacgct gatggaagaa tatgtcagct ttgatgcatt tgatgattgt tatatcacga
    27541 ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa tagtttttat ctgccaaatt
    27601 atattttaga cgagctattt aagaatatct cccatcgaaa agtgatttta tctccagatg
    27661 aaacaatgaa gattggtgga tatgcttata aaggcggacg cgtgatttta gagaatttta
    27721 cggatagtct gactgaaatg atcaatgaaa aaaaagctca gttgaagact atgacgcgcc
    27781 agccgattgg tttcttctcg aaagatgatg tttggagctg atattttgaa gcattgccgt
    27841 tgcacatgag agcgtggtaa tgctttcttt ttttcttaat gatacaatag aactgaacgg
    27901 agtgaagaaa gatgttacaa atctatgccc atcgcggaag cagcggcacc cacccagaaa
    27961 atacgcttcc tgcatttgca gaagctgtac gagtaggtgc agatggtatc gaactagatg
    28021 tccatctaag caaagatggt tatttgatcg ttatgcacga cgaagaagtg gaccggacga
    28081 ctaacggtaa agggctgatt cgagaaaaaa cattggaaga actcaaaaaa ttgaatgcag
    28141 ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc tgaattgaga gaagtgttag
    28201 gactgctcct ccaaaaaaat taccgaggga tgctaatgat tgaaattaaa actgatcagt
    28261 atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt aaaaagtaga gattggcctt
    28321 ttcaacatgc atattgcagc tttcatttgg attctttgga aaaaatcgca ttactggaac
    28381 cggagataca gctggactta ttgatgagta cttctgataa aaagatccaa ctagcagcag
    28441 aaacccctta tatagaagga gtacatccaa aaaatacttg gttgttcgaa catccagtcc
    28501 aggcagctga ttttccaaaa gcaattcgtg catggacgat taacgatgaa gaagaaatgg
    28561 ccaaatgtca agaactcaaa atagatggaa taataactga ttttccggag aaagctcttc
    28621 ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa agtattagtg atcgtcggtc
    28681 cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc acaactattt ggaggagaga
    28741 tcatcagtgg tgattcattg caaatctata aaaatctgga tatcggtaca gcaaaaatca
    28801 aagaagaaga gatgaataat gttccgcatc atttgattga tgtgatcgaa cctactcaac
    28861 gttactcagc cgctgatttt cagcattcag gtcgacaatt gattaccgag attgcgaacc
    28921 gagggcacct acctatcata gctggtggta cgggtctcta tatccaatcc cttctatatg
    28981 attatcagtt aggagcgaca gaagaagcga atcctgagat tagaaaaaag tatgaaacgt
    29041 tagctgaaac aattggttcc caagcattat ggaatcagtt gaaagaaaaa gacccggcag
    29101 ctgctgagaa aatccactgg aacaatcaac gcaaagtgat aagagctctg gaagttttag
    29161 agacgacagg acatagtatc acggcaccta aagaagagcc aaagcaattg tatgattacc
    29221 tcatgattgg attagatacg aagcgtagta ttctttatga acgaatcaat caaagagtcg
    29281 atttgatgct agcagaaggt ttagtagcag aagcgcgtaa ggtgtatgag cttggagaag
    29341 tccaggccag tcaaggtatt ggatataaag aatttttccc ttattttgaa gggaaagaaa
    29401 cattggaaga agcaacagaa caagtcaaac tacattcgag aagatacgcg aaacgtcagt
    29461 tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga tcttataagt gagccaaatc
    29521 aaaaaagtca gattgagcaa acaatcaaaa aatggctgga ggcggaataa aatggaagaa
    29581 aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc gttacttttc agagtcaatg
    29641 gaagagctag ttcagctgac gaataccgca tcaggagaag tcgtattcac aattacacaa
    29701 aagagaccac aagtggatcg gcaaacgatt atcggtaaag ggaaattaca agaattagtt
    29761 caacaagcgg atgcgcatga agcggatttg atcatcttta atcatgagct aactccgaga
    29821 cagagccaac tgattaccga tgctgttggt cttccggtaa ttgatcgcgt gcagcttatc
    29881 ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga agctgcaggt tgaacttgct
    29941 cagttagaat acctgctgcc tcgtttagta gggcaaggga aaacattatc tcgtcttggc
    30001 ggtgggatcg gaacaagagg gccaggtgaa acaaagttag aaacagaccg ccgacatata
    30061 cggaataaaa tttttgctgt taagaaagaa ttgaaagaag tagaagctca tcgagagcgc
    30121 aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg gacttattgg ctacacgaat
    30181 gccgggaagt caactatctt gaatctattg acacaagcgg atacttattc gaaagatcaa
    30241 ttgtttgcaa cgttagatcc attgactaag agatggcgtt ttgctgaagg atttgagatt
    30301 actgtaacgg ataccgtcgg gttcatccaa gacctgccta cgcaactgat cgatgctttc
    30361 cattctacat tagaagaaag tcaaaatatg gatcttttgc tacatgtagt cgatgctagt
    30421 tctccagatc gtattttaca ggagcagact gtcttaaaat tgatggatga actgaatatg
    30481 aaagaaatgc cgatactgac tgtttacaat aaggcagacc agatcgaccc agcgatgttc
    30541 acgcctacgc tattcccaaa tgtattgatt tctactcaga cgaaagaagg taagatggcg
    30601 ttgatcgaag caatcaaacg tcagctgatg gaactaatga ccccttatac actttctgtg
    30661 ccaagcaacg aaggacagaa attaagcgag ttgagaagac aaacaatggt tttggacgaa
    30721 gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt ttgcaaaaac caattcaaaa
    30781 tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DACCZY010000022        30081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCZY010000022 DACCZY010000000
VERSION     DACCZY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.022"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..1279)
                     /locus_tag="IVZ67_000789"
     CDS             complement(59..1279)
                     /locus_tag="IVZ67_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8164887.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(1294..2382)
                     /gene="murG"
                     /locus_tag="IVZ67_000790"
     CDS             complement(1294..2382)
                     /gene="murG"
                     /locus_tag="IVZ67_000790"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8164888.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(2408..3769)
                     /locus_tag="IVZ67_000791"
     CDS             complement(2408..3769)
                     /locus_tag="IVZ67_000791"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8164889.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(3783..4745)
                     /locus_tag="IVZ67_000792"
     CDS             complement(3783..4745)
                     /locus_tag="IVZ67_000792"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8164890.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(4771..6963)
                     /locus_tag="IVZ67_000793"
     CDS             complement(4771..6963)
                     /locus_tag="IVZ67_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8164891.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(6964..7365)
                     /gene="ftsL"
                     /locus_tag="IVZ67_000794"
     CDS             complement(6964..7365)
                     /gene="ftsL"
                     /locus_tag="IVZ67_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8164892.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(7370..8329)
                     /gene="rsmH"
                     /locus_tag="IVZ67_000795"
     CDS             complement(7370..8329)
                     /gene="rsmH"
                     /locus_tag="IVZ67_000795"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8164893.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(8350..8781)
                     /gene="mraZ"
                     /locus_tag="IVZ67_000796"
     CDS             complement(8350..8781)
                     /gene="mraZ"
                     /locus_tag="IVZ67_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8164894.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(8951..9319)
                     /locus_tag="IVZ67_000797"
     CDS             complement(8951..9319)
                     /locus_tag="IVZ67_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8164895.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            9490..10446
                     /locus_tag="IVZ67_000798"
     CDS             9490..10446
                     /locus_tag="IVZ67_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8164896.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10519..10635
                     /locus_tag="IVZ67_000799"
     CDS             10519..10635
                     /locus_tag="IVZ67_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8164897.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(10690..12372)
                     /gene="recN"
                     /locus_tag="IVZ67_000800"
     CDS             complement(10690..12372)
                     /gene="recN"
                     /locus_tag="IVZ67_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8164898.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(12390..12839)
                     /locus_tag="IVZ67_000801"
     CDS             complement(12390..12839)
                     /locus_tag="IVZ67_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8164899.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(12946..13764)
                     /locus_tag="IVZ67_000802"
     CDS             complement(12946..13764)
                     /locus_tag="IVZ67_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8164900.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(13773..14663)
                     /locus_tag="IVZ67_000803"
     CDS             complement(13773..14663)
                     /locus_tag="IVZ67_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8164901.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(14665..14889)
                     /locus_tag="IVZ67_000804"
     CDS             complement(14665..14889)
                     /locus_tag="IVZ67_000804"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8164902.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(14894..16231)
                     /locus_tag="IVZ67_000805"
     CDS             complement(14894..16231)
                     /locus_tag="IVZ67_000805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8164903.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(16232..17086)
                     /locus_tag="IVZ67_000806"
     CDS             complement(16232..17086)
                     /locus_tag="IVZ67_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8164904.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(17183..17632)
                     /gene="nusB"
                     /locus_tag="IVZ67_000807"
     CDS             complement(17183..17632)
                     /gene="nusB"
                     /locus_tag="IVZ67_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8164905.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(17625..18050)
                     /locus_tag="IVZ67_000808"
     CDS             complement(17625..18050)
                     /locus_tag="IVZ67_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8164906.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(18296..19360)
                     /locus_tag="IVZ67_000809"
     CDS             complement(18296..19360)
                     /locus_tag="IVZ67_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8164907.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(19532..19777)
                     /locus_tag="IVZ67_000810"
     CDS             complement(19532..19777)
                     /locus_tag="IVZ67_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8164908.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(19916..20290)
                     /locus_tag="IVZ67_000811"
     CDS             complement(19916..20290)
                     /locus_tag="IVZ67_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8164909.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(20373..21287)
                     /locus_tag="IVZ67_000812"
     CDS             complement(20373..21287)
                     /locus_tag="IVZ67_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8164910.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(21308..22111)
                     /locus_tag="IVZ67_000813"
     CDS             complement(21308..22111)
                     /locus_tag="IVZ67_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8164911.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(22144..23133)
                     /gene="manX"
                     /locus_tag="IVZ67_000814"
     CDS             complement(22144..23133)
                     /gene="manX"
                     /locus_tag="IVZ67_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8164912.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(23236..23727)
                     /locus_tag="IVZ67_000815"
     CDS             complement(23236..23727)
                     /locus_tag="IVZ67_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8164913.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(23918..26752)
                     /locus_tag="IVZ67_000816"
     CDS             complement(23918..26752)
                     /locus_tag="IVZ67_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8164914.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            27371..28165
                     /locus_tag="IVZ67_000817"
     CDS             27371..28165
                     /locus_tag="IVZ67_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8164915.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            28261..28563
                     /locus_tag="IVZ67_000818"
     CDS             28261..28563
                     /locus_tag="IVZ67_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164916.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(28599..28970)
                     /locus_tag="IVZ67_000819"
     CDS             complement(28599..28970)
                     /locus_tag="IVZ67_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164917.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(28972..29373)
                     /locus_tag="IVZ67_000820"
     CDS             complement(28972..29373)
                     /locus_tag="IVZ67_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164918.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(29387..29794)
                     /locus_tag="IVZ67_000821"
     CDS             complement(29387..29794)
                     /locus_tag="IVZ67_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164919.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT         8605 a         6382 c         4924 g        10170 t
ORIGIN      
        1 ttctaccata aattactggt ttttagaaag tacattctta ctttttaaag ttgttttatc
       61 aattttcatt taaacttgac ggagttgtac ttggattatc ttctatttga cttgtttcac
      121 ttgaatcatt tgaatcagat gatccgtttg tgctagacga tccaggtgtt tgtgcttttt
      181 gcgtttctgc ctcttcactt gattcgtttg tttgatccgc attttcttca ctttcagttg
      241 tttcttccga ttggcccgat tcttccgttt cttgagtgct accgtaagga tgcgagaaga
      301 taccaacttc catgtcgatc acaccttttt catccatatc tttggctact tgcgaataat
      361 atttcatttg gttcgcaaga ttattgatat ttacaataac ttgattgccg tcattcatat
      421 tcaattggat cagattttta ttgcttgctc ttggcgtgta cttgatttca gaaattgctt
      481 tttgtaattc tgatgataac tgattgtatt gctttgttaa ttctttgatt tttgcagagt
      541 ctttgaactc ttccaagata gggagatttt ttgtaggatt tgctactttt tcatctaata
      601 ctgttccatt ttcaatcact gggtagtatt gattgttttt tgcgatcaaa gcaatctctt
      661 tatattctgt tacatccaaa tcgaattcat tcatcccttt gaaatgtata tttgctgttt
      721 cgattcttgg ctgtgctttt ttaaggcgat cgatataagt agatcgatga aaatactgtc
      781 cccaaacatt ttctcctaat gacaattttg tatcagagat gatttcttgg ctgttgactg
      841 tcttattgcc tgatactgca actgcttgta atttgctata aggagaaaca taatagatca
      901 aaaaaatcag cggtataccc aatacagtga tgatcaaagc taaccgacga aatagtactt
      961 tgtgccgttg actttttaaa ttgggcagcc ggttcaaaaa agaaccgtta tatggtccac
     1021 cgtttttctt ttgcttgatc tcttcaaaaa attcgatttt tttctcggac tggctttctt
     1081 cttgctgttc ctcttgaacc gttttttctt cttcagaatc tgaaaggggt gtatctaatg
     1141 ttatgtcttc tttttttggc tcttggtttt tcatctcttg ttctgccaga tattttcggt
     1201 tagccttttg ccaaggagtc aaattgtttt catcagaaga cggatgagac aatttttttg
     1261 gttctttttt cttactcact aagaccaccg cctttatttt ttgattattt gttttactaa
     1321 gtcataaaga cgttttgaag catcagggat tccttgttct ttagaagctc ggcacatttg
     1381 cttttgcaat tcttcatcac ccatgatttc gttgactgtc tgacttagtg attcacctgt
     1441 tagttcatta tctgcaatca tttttgctgc tcctgcgtga acaagactca ttgcattttt
     1501 agtctgatga tcattcgtaa catatgggct gggaaccaac actgccggta accccagagc
     1561 tgtgaattct gcgatcgaag ttgcaccagc acgccccacc agcagatcac tggaagccat
     1621 gacctctgcc atatttttga tataaggctg gacactaata ttttgtattt caggatttat
     1681 tttatttagg atctcttcat aatatctatc tcctgaagca tacaaaactt ggtaagaaga
     1741 aggaatatat tcacttaaaa aaactgatac tgcttgattg attttaagtg ctccttgact
     1801 accgccaaaa atcaagactg ttttcttttc tggatccaaa tcaaatgttt tcaaaatatc
     1861 tgatttttta gtatctgcta cttcttgtgc tcgaggatta cctacaagaa ctgctttgtt
     1921 ttcaggaaaa aaggaagccg catctttaaa tgaaagagcg atccgatcca catatcggct
     1981 taaaaattta ttggtaacac ctggtacact gttttgttca tggataactg ttggaatacc
     2041 cattttggct gcagcgtaca ctaccgctcc ggaaacatat cctccagtac cgataacgat
     2101 atctggttga aaatcactga ggattttttt tgcttgacgg atactcttaa gaaacaatcg
     2161 gaccgtcttg atgttatgca tggaaatttt tcgtttaaat ccttgaatct ccaaggtatg
     2221 gaaaggcatt cctgtatcag ggacgatttt gttttctaga cctcgttttg ctcccacata
     2281 catgaattca gcattcggtt cttgagtctt tacataattg acaaaggcaa gagctggata
     2341 aatatgtccc cctgttcctc cgcctgtaac caaaattttc attttttatc gctcgcttct
     2401 actttttcta ttttaatcgg ttcacagctt ccatgaatct gtttcctcta gtttcaaaat
     2461 ttgggtattg atcccagctg gcattcgctg gtgataataa aactacatca ccatctgtac
     2521 tgttttctaa tgctaaagga actgcttgtt ctacagaatc ggctgttact attgtctcga
     2581 tgcctgcttt ttttccagca tccatcaact tttcttttgt ttggccaaat acgaccattg
     2641 ctttgattcc ttttaatgaa ggaattaatt catcaaatgt atttcctctg tctaatccgc
     2701 cagccaataa cacgacctta gacgtttcaa atccacttag agccatttta gtcgctaaaa
     2761 tattggttgc ttttgaatca ttataaaatt ttctgccttg gatttctcct acatattgcg
     2821 tacgatgtgg tacgccatgg aaaaaatgca acgtttctcg tatagcttca tttgatatac
     2881 catacaattt tgctacagaa atagcagcta gtgcattttc tacattatgc ttacctggta
     2941 cgccaagttc agatatttcc attatttttt cttttttata gtatattgag taatcgtcag
     3001 catacacacc gtcttccaac acttcttttg tcgagaaagg cagtacgcgt gcttttgttc
     3061 tttgcgctaa ttcttgtagt tctgactgat tccagttaag gatcagataa tctttttctg
     3121 tcatgttttt ttgaaggttc catttagctt tgacatattc ttttcttgta ccatgataat
     3181 caatatgtgc ttcatagata ttggtaatca ccgctatatg agggcggaaa tctgtgattc
     3241 ccatgagttg gaagctagac aattccatca caattttatc ttctgatttt gcttcttgcg
     3301 ctacaccact tgctggatac ccaatattac ccgctaaacg tgcaattcct tgatctgctc
     3361 ctgcattcag tagtagtcct gtcatcgttg ttgtcgttgt ttttccgttc gttcctgtaa
     3421 tcccaataat tgggcattcc gctacttcgt aagctagctc aacttccgta attaccggaa
     3481 tacccatttc ttgggctttt gctactaaag gatgactgta gggaatccct ggatttttga
     3541 ccatcaatga aaattcttca tccaacaatt cgattggatg actacctgtt attactttta
     3601 tccctaaaga aagcagctct tgtgcttccg gattttcgtc aaaaggtttt ccatcattaa
     3661 cggtgacaag tgcgcccaat tcatgtaata atttggccgc acttactccg ctttttgcga
     3721 gtcctaagac aagcactttc ttattttcat atgttgatat ttttttcatt tcccgtcatc
     3781 tcctaccaaa cgatccataa tgtgatcaac gaagtcacaa tgctgatcaa ccaaaaaact
     3841 gtatcaatct tccactccga ccatccgcac atttcaaagt gatgatgaat tggagacatt
     3901 ttaaaaattc gttttcctgt aagcttaaag gaagtaactt gaagcatcac acttgctgtt
     3961 tccataacat agatcaaacc aatcaaaagc aatgtccatt cttggtttag cattatagaa
     4021 attgccgcta aaagtccgcc caacgctaat gaaccgacat ctcccataaa aatcttggca
     4081 ggttttctgt tgtagacgaa gaaacccagt aaacctccta aaacacttaa gcaaatgatt
     4141 aatacatcat actgatgctg atgccaagca attagaccgt aagctgcaaa agagatactt
     4201 cctaagccag caactaatcc atcgatgcca tctgtcagat ttactgcatt tgaaaatcca
     4261 acaagccaga agagagcgaa aataccataa atgattccta aaggtagatc tagtccgaag
     4321 aaattcaata cacctggata accttcagaa cggtaaacaa gataaaagat gattccccct
     4381 agaatctgtc caatcaattt ctgcttcgag ttcagtccca tgtttctttt cttgaatatt
     4441 tttataaaat catcaagaaa gccgattact ccgtataacg ctaatacaaa aagcaagatg
     4501 aaaagagtag gagttagctg tttttgccaa gctccaaccc aaattcctgt caaaatagag
     4561 ccgatcagaa acactaagcc gcccattgta ggagttccgg ctttgctatt gtgccacttt
     4621 ggtccctcct cacgaatcgc ctgaccttgc tttttcattt gaaaataacc aataaatagc
     4681 ggcattgcgg caattgtcat tgcacaactg ctgacaatgg gaatcaatgc ttgtgtccat
     4741 tccatgctct tttctctcct atttctcttc ttattcttct aaagtaaaac taagtttgtc
     4801 ttcagtgatt ttttcatatg gagcaagttc ttgtttgaca caataaccat ctccatcaaa
     4861 actaacttca ataccgagga ttttccccag cttcatgata tctgcttttg accatcctgt
     4921 tacgtcaggc atcagcttat caccaccagt atataggatc agtttttctc cagagatcaa
     4981 ctgatcaccg ttggcagtag attgtttttg tactttttta ccattaccga tcactacagg
     5041 ttgcagtcca cttttttgag catcagctgc tgcaacgtcc gcttcaagat tacgataatc
     5101 ggctacggat actttttctg tttttgtttc tgtatctgaa tcttcttcag tttctttaaa
     5161 atccattgcc cgtttcatca atggatttgc gattttagcc aatgccattc gatcatatgt
     5221 cttagggtgt ttcattgtta gatacaagac atagtctgga tcttcagaag gaaccatctc
     5281 cacaatcgaa tacaggtagg ctgtatctcc tgtttgatat ccccctgtat cagaagcgat
     5341 ttgggctgtt ccgttttttg ccgatacatt gtatccaggt acactatata caccataagc
     5401 actaccgtag ttttcgcttt cgacaacatc tctcatatat tcacggactt tttctgttgt
     5461 ttcttttgaa aaaggttgcc ctagaacttc tgtttgagtt gttcgctctt ctccagtttg
     5521 gggatccacc actttgctga tatatcgagg ttgaatcatt gtaccgttat tcgcaataga
     5581 agtgaaagct ttcatcattt ggaaattcgt cacacctacc gcttgtccat aagcactcat
     5641 tgctcggtcg acaatattta atgtaggtaa agcaccgttt acttcatcat ctaatccaga
     5701 atgagtactt tgtccaaatc ccagtttttg taaatagtta taccatcgtc cgcctaatcg
     5761 ctgttccagt atgaccatcc cgacgttact tgaccatgaa agagcttgac gcatggtcag
     5821 cacacctttt tcaccaaagt catggtcatt gatcgtagca tcttctactt ggatttttcc
     5881 agattggaag gtttcattct cgttgaattc tccttcttcg atagcagctg cagtagtgaa
     5941 aactttcatg gttgatcctg gttcataact atcttgtacc aagaaattac gccagatagc
     6001 atcttttcca gtcaatcctt ccattgtttc aggattgaaa gtaggtcgtt gtcccattgc
     6061 caagatttct ccggtttttg ctttcatcag gactgctgtc aattcttctg gctgatattc
     6121 ttcatttact tgatccatca gtgtttctaa ataactttgt agtctgctgt ctaaagtcgt
     6181 gtagatatct tgtccgtcga cagctttttc ttcttctgcc actgtacctg gcagaggatt
     6241 ttgaaaatta tctttttgat aaataatctt accatcttta ccgctcaaga tatcattgta
     6301 agcagactcc aaacctagtt taccaactaa gccattctca tctttgtctg gaactgcgta
     6361 tccaataaaa tgagaagaaa agacgccatt tggatacatc cgagcctgat ggtcgacaaa
     6421 ataaagacca gcgacacctt cttttttcat gtccgcttcg atgttttgct tggtttcttg
     6481 ggagatattt ttcccatagc taccaaattc aacttgatac agctcttttt ttgcgccgtc
     6541 ctctaaaata ttcagtgcat cttttttgtc tatagaaaga ttcttatgca ggatttctgc
     6601 tattttatca aaattctttt cttcaacata cagtttttta tccccagagg tatatgtttt
     6661 tgagagtatc gctttaattg aataagagga ggcatcttct gcgagcgcca caccgttacg
     6721 atcataaatc gttcctcttt ttgcttttac tacttcactt ccttggtaca actgttttgt
     6781 tttttcagct aatgaggtac cagcaacatg acctccaata acgatatacg agaaacggac
     6841 ggcaaataaa aagaacaatc caatactcgt agcaaataaa atgataccta cttttttgcg
     6901 attgttcatc ggatttagat ttttcttttc cataaaccgc cgaattttat ttttaagact
     6961 catttacttc actttcctta aattgtcgtc attaatagac aatccttttt cttctgcgat
     7021 ttgtttaatc cgttcagtac gagaaagttc gtttttttct tgttctaaac tcgttttttc
     7081 ttgcttttta tcattgattt ctgtttgtat tgacgtgatt tctttttcag ctccgctgat
     7141 atttgtgcgc agcataactg ttaaaacaca taaagcaaca atagcaatcg ccacgcttgc
     7201 cacagcaatt ttttccaaag cagaaatctg ctttaatcgt tttgccggtg attgtggaag
     7261 aatgatttct tgattcgaag aagaatcagg catttgttgt tccggctgat ccaccaattc
     7321 aggtaaatca tattcgtaat cttgcatttt ctttaattct gccatcattc tactcctctt
     7381 tcattcttat tttttctgca atacgtaact ttgcactgcg tgatcgattg ttttctgtta
     7441 gctcttcctc tgaaggaagg attggtttac gagtaatgac tttcagttct ggttggaatt
     7501 cgtcaggtat gacaggaagt cccggtggta gatcttgtac cgtactatat tctttaaaca
     7561 tacttttgac aattctgtct tccaatgaat ggaatgtgat tacgctgatt cttccattga
     7621 ctttcaataa ccggattgcc tgttctaaag aggcctcttc ggcaccgagt tcgtcattta
     7681 ctgcaatccg cacagcttga aaaattcttt ttgcaggatg cccgcctttt cttcttgcgg
     7741 gagctgggat gacttgttta atgatttcga caagctcacc agttgtttgg attggttttg
     7801 tttttcgtac tcgttcgatt tcacgagcaa tttgtttgga aaatttctcc tcgccatagc
     7861 ggaagaaaat ctttactaat tcatggtagg aatattcatt aattacgtga taagcggata
     7921 aaggagctga ctgatccatg cgcatatcca acggtgcatc ttgatgataa ctaaatccac
     7981 gttctgcttc atctagctga ggagaggaaa cccctagatc atacaaaatg ccgtctacct
     8041 tatctacatg ctcactcaag cgttcttcta attctctgaa gttcgctttg ataaatgtga
     8101 ccataccttg ttcgacataa gaagctaaac gcagcttggc gtgatcgatt gctttttgat
     8161 cttggtcaaa cgcatacaga tgccctttag aacttagttg gcttaataaa tattcactat
     8221 gtccggcacc gccgagtgta cagtctacat aaacgccatc cggtttgatg ttcaatccat
     8281 ctaccgtttc tttcaacatg actgtatagt gttgaaatgt ttcagtcatt agtcagcctc
     8341 ttcctattct tatagtccga aatcaatcat actttcagca atttcgtcaa agttctcttc
     8401 tgctgctgca gaaaaatctt gccatcgtgt ctcatcccaa atttcaattc ggttagatac
     8461 accgatgact acgcaacctt ttgtcaaagc tgcataattt cttaacgtgc tgggtatatt
     8521 gattcgtcct tgtttatcaa gttcgcattc agtggcagcc gaatagaaga atcgcacaaa
     8581 agtacgtgcg tcttttttag ccaacggcat ttcgttcagt ttagcttcca gattttccca
     8641 ttcgctcatc ggatagccga aaagacagcc atccaatccg cgtgtcagaa caaacttttc
     8701 gccaagttct tcacgaagtt ttgagggcac gattaaacgt ccttttgcat caatattatg
     8761 ttggaattca cccatcaaca ttgaaagccc cccattcctc cactaactaa aattagtcta
     8821 ccacaatccc ccactttcta ccaccttttc tgggaaatcc gggaacaact ttttttattc
     8881 ataaacattt ttttaaaaat caagctgcaa aaagcctttt aaaagacttt ttacagcttt
     8941 tctatttttt tcataggtaa gaaagtatgt tcacaatgat caagacagcg tacaagacga
     9001 ttgtcaacag aaaaaccaaa cgccaagtca tcttgaaata acgcggataa ataatttctt
     9061 cataataata tgcttggaaa acagcaatac aaattcccat tagcaaaaca gaaataaaga
     9121 aaaacggtaa gatagaaatt gtccctgtat ttctagacaa tgcatgcata ccgccccaca
     9181 aaaaaggaat cgccaaatca ggggctttta ttttaaaacg acgatttaac gaaaataatt
     9241 ttacaagaaa ttggcaacca aacaaaacga ttaccgggta aatataccaa aacaagataa
     9301 gtggtgtgaa tgtgcccatg aacgtaaact ccttttttat cgttgcttca agtatactgt
     9361 atatacttta cacaactgtt tcattttatt taaatttatg cagttatatt ctatatctta
     9421 caaacacatt ccatgtattc caattcccta tccgttataa taaaagaaac aggcaacagg
     9481 aggaaaatca tgatcaaata ttttacatta gataatgaga aactgcgtaa taccggaaag
     9541 gaaactgacc aaacgatctg gtattgtgta gaacgtccta atcaagaaga gattcatcag
     9601 ctgacacatc aatttgaaat tccgtcagat tacatcactt ccatattgga cgatgcagaa
     9661 aattcccgta cagaagaatt caatcaatca aagttgacaa agccagcgtt attattgtta
     9721 caatatcctt ttgctaaaac aagtcctagc ggttattttc aagtggatac atacccgctc
     9781 tctattattt tgacgcccaa aaagaagctt atcacaatta cgaatcatga accactgttt
     9841 ttaaagaaag tctttagcca gacctttcca aaaagtgata tttcgcctaa tctcaatatc
     9901 ttctttcaat tcatttggca ggtcacactc tcttacaaca gttatttagc tacgcttcaa
     9961 tctcaatgcg atactttgga agccaaactt caagtatcca ctgaaaacaa acaactttat
    10021 caaatcatgg atatccaaaa aagcctcgtt tatttcagag aagcgacttc tgctaattta
    10081 gatgcattga aagaactagc gcaaaataaa cttttccaag aaaatcatgc tttgaaaaat
    10141 catttacgtg atgtattggt ggaaacagag caggcaatga ctacttctcg cattcaatta
    10201 aaactagtag aacaaatgaa tcagactttt tcagcaatcg tatcgaataa tctgaataat
    10261 attatgaaaa ttttaacttc tttaacgatt attttaacta taccgacaat cgtcggcagt
    10321 atctatggaa tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta
    10381 gttctattaa cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt
    10441 ttataaaaaa gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta
    10501 atgacgaggt tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca
    10561 aaatttgtca tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta
    10621 atgagtgtca tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg
    10681 cgaggtggct caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag
    10741 ttttgtaatc tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt
    10801 caactctgaa acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg
    10861 atagtcggcg actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt
    10921 cgatatttga gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc
    10981 cacttcatca aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc
    11041 aagcaacatc cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc
    11101 tcctggattg gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct
    11161 attttcttct tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa
    11221 ttcatgcaga atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc
    11281 tgcggcttgt tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa
    11341 ttgaccttct gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa
    11401 tattgactca atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg
    11461 cgtaacttct tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc
    11521 gccgattgca tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata
    11581 ctccacatct agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac
    11641 actgttttgt tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt
    11701 ttgataattg ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa
    11761 atcagcagca gctatttctt cttgctggaa atggagcata tctattctct gaacatagga
    11821 tttttcattt tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc
    11881 ttgctgataa ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc
    11941 aagatgtgat tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa
    12001 ataactgccg attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac
    12061 attttttcca gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat
    12121 ccctaattca accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc
    12181 aagcacacat ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa
    12241 cagacctaaa gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt
    12301 cattccttcg tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat
    12361 ttcttgtagc atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat
    12421 aaaactgcct ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc
    12481 gaaaataatt ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc
    12541 ggcaatgttt tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt
    12601 aatagttttt ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg
    12661 tagccgccat ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc
    12721 gtagcttgag taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt
    12781 cggatatctt gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt
    12841 acgtcttcgc tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat
    12901 aaagaagaat ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa
    12961 ctgttcgtgt gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct
    13021 ttcgggtact ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg
    13081 agaataactt aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc
    13141 taaaactttt tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct
    13201 gcctgcttcg aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc
    13261 atgtaatgga ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc
    13321 tggaattcct aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac
    13381 gacgactcta ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc
    13441 ataactcatt tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc
    13501 aatatctaac aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa
    13561 ttttaatccc cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact
    13621 cactggaatt ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc
    13681 agccatcact gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa
    13741 tacatctacg cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc
    13801 atctggcgca tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc
    13861 ttgttggcgg ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa
    13921 cgcgggtaag tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc
    13981 gttgtaccaa tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg
    14041 gctaatcgtt gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc
    14101 agcaaggcat aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct
    14161 aaagaaagct ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt
    14221 gatcccgcac aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt
    14281 gccatactga ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat
    14341 gcttcgccga atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc
    14401 gcaggcaaat catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact
    14461 tgatagacgg gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag
    14521 ggacgtattc ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta
    14581 tccgttgtat gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata
    14641 gctgaaaagt cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc
    14701 atttgattct gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca
    14761 atgtttgctt aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc
    14821 tccttgttct aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg
    14881 attttccata tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat
    14941 cttgataatg gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt
    15001 gatcatttaa cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat
    15061 caagagattg aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt
    15121 tctcttctaa gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa
    15181 gcggagccac ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat
    15241 aaatttgctg tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac
    15301 cttcatacaa tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt
    15361 cttgtttgcg ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa
    15421 tgcgcatcaa ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg
    15481 cagcccgaac atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg
    15541 aaatcaccgg cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc
    15601 ataaatcctc aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct
    15661 cttctactct ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg
    15721 ttgggaacaa aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc
    15781 ggataaccgc tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca
    15841 tttttttagg cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt
    15901 cttgatataa agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac
    15961 ctcccgcttc atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct
    16021 ggaatcgtaa tttttggaat gctcctttga acataatagc agatattttt gcatggtcat
    16081 cttttaagct gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg
    16141 ttaaatatac gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta
    16201 atgtagtgac agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag
    16261 attttacggt ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag
    16321 gagtgatcca gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac
    16381 cgttagcatc acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt
    16441 ttgtgacaaa atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt
    16501 cagccagatt ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta
    16561 gctgagccat cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg
    16621 ccggatcgat agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct
    16681 tgtccggttt acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt
    16741 tttcagggcg gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt
    16801 ggaccaatat accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt
    16861 cgtcttcaga aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg
    16921 ctacacgttc tttatttctt acatagattt ggctggctgg attttcccct accaataaaa
    16981 cgactaatcc gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg
    17041 cctgcatttt ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag
    17101 atattttttg tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg
    17161 attcatagaa aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata
    17221 aaacaccgtt gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg
    17281 cttcatttaa agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga
    17341 tacgcaagat caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat
    17401 gtttttcaat cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat
    17461 caagataaac aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt
    17521 cgattgcgtt gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata
    17581 aagcctgcaa tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc
    17641 tcttcgtctt caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct
    17701 actacatgga tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg
    17761 acccgttcct gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag
    17821 taaagatcta ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct
    17881 gcacgtccta ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct
    17941 tctactttag aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc
    18001 tctcctaaat cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc
    18061 ctccctttgc ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg
    18121 tggtaagaaa actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac
    18181 tttattatct cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt
    18241 ctataacaaa gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata
    18301 agatgatcaa ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca
    18361 agtcatcctc aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga
    18421 tcacatttcc tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga
    18481 tctctaaacc gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg
    18541 aaatataatc tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat
    18601 caataactgc taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag
    18661 gatcaccaat agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa
    18721 agtccaatgt gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca
    18781 tagccgaacg caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag
    18841 aacgcatata aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca
    18901 ttttcaggat gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga
    18961 tttcttcttc atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt
    19021 ccgcatcgat gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa
    19081 aagctaatga gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc
    19141 cgacattttt aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa
    19201 aatcagtaat aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa
    19261 agttcgttaa gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt
    19321 cttgcatttt tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt
    19381 tgcacgcttt tttccattat agcaaacttt tcttcgtaca cgacagttaa ataaaaattt
    19441 ccggaaaact tctttttatt ttaaataaat aaagccaaaa aggagtcaca gtctcgctgc
    19501 aactcctttt agtttactat tttgtaaaaa cttatgatcg ttttccaaag aaaaatgaga
    19561 caactgctac caaaatcact gcgcctaaaa tagatggaat aagtgccata cctgcagctt
    19621 ttggtcccca ttcgccgaaa atcgcttgtc caatacttga accgactagt ccagcaatga
    19681 tattcgttac acagcccatc gaatttcctt tactggtgat cgcacctgca attgctccga
    19741 tcacggctcc aacaatcaat gtccataacc aactcataat ataacctcct tctttgctaa
    19801 tcaattttag catatgggat ttaaagggga caaataattg gttttgctat ttcatatttt
    19861 cttctgcaaa gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt attttttact
    19921 tgcgtttaaa catactacga ataccacgtt tcactacatc aaagaaactc aatgattgta
    19981 ccattctatc ttcatctaca tattctctga tcgcacgaag cactttttta ggttctttgg
    20041 aagaaaatgt gtatgtacca ttttttttcg tttgaatggc atatcgagga atccattttc
    20101 cctttagcat gactgatgct agtacataat ccacttcttc ccatggaatt tggatatatt
    20161 tgcgtctatc tcgcgcatgg taaaactcaa aaccattatt acctatcatg atttgcccgt
    20221 aatctgtcag acccgtaaac gaagtagcat caattaccaa gtctactttt gtattgattg
    20281 attgaaccat tttctccctc cagatatttg tttatgaaca gtatactata taaaaaaagc
    20341 tcgggacaac gcccgagcta tatttttatg gattataaaa gtccgatgac gtgtccaaca
    20401 acaccgacta cgaataaacc aaggatgata acgattggag aaaccttttt cttaagcaac
    20461 cacatacata acaatgttaa tcctaatgca gctaaaccag gaatcaattg gtctaagttg
    20521 tcttgtaatg ttgttacctt ttcaggagaa agggctaagc cattattaac ttgttcaaat
    20581 gcactcttga ttccttcacc acctgaaggc aatttatccc attcgatgta agcaccttta
    20641 tctaatttta cagttgatac aactggtaag aatttgattg atacccatct ttgtacaagg
    20701 gcagctaata cgaacatccc taaaattgaa gcaccttttg ttacgtcttg taacaatccg
    20761 ccagaaaggt catcagtaat tttagaacca gctttgtagc caaattcttg tgtataccac
    20821 atgaatgccc aacgaataat gttccaacct aagaagaaga tgattggtcc aaggatattt
    20881 cctcccatag ctaatgaagc acctagtgca cctagcattg gacgtactgt aaaccagaac
    20941 actgggtcac caacaccagc taaaggaccc atcataccta ctttaacccc ttgaatcgct
    21001 acatcatcaa caggcgcgcc attcgcacgt tcttcttcca gagccaatgt aacccctaaa
    21061 attggagaag caatgtatgg gtgtgtatta aagaattcca agtgacgttt taatgctgca
    21121 gaacgttctt cttttgtttt gtataatttt ttgattgctg gaatcataga aaatgcccag
    21181 ccaccgtttt gcatacgttc gtagttccat gaaccttgaa tgaaggtaga gcgccacgca
    21241 acagctaaac gatctttttt tgataatttg attttttctt ctgccatttt attttcctcc
    21301 ttcaagatta atagtcattc aaaatgtcgc ctaaagggtc accagagttg cttccgcctc
    21361 cattatttga tgaaccaccc attttagaaa ggttcaagta aataagagct aatgcaacac
    21421 ctaaagcacc tagagcaatc aatgttaatt gagaaattgc agctacaacg aaaccaataa
    21481 cgaagaatgg ccatacttct tttgtagcca tcatgttgat taccaaagcg taacctacag
    21541 ctacgaccat tcccccaccg atagccatac catctgttaa ccatgctggc attgattcaa
    21601 ggaatgattg cacagtttgt gctgggataa atagcaacgc tgctgctggg accgcgatac
    21661 gaaccccttg taaacataca gcaatgatgt gccacatttc aaccttacgg atgtttcctt
    21721 cttcagcagc cgcatccatc aaatgaacga ttggaacagc gattgtacgt acgatcattg
    21781 ttaagaacaa ccctgcaaca gccaaaggaa cggcaatcgc gatcgcagaa ggaacacctt
    21841 taacaccttg tccacctaaa actaaaataa ttgctgatgc tactgatgct aatgcagcat
    21901 ctggtgctac agcggcaccg atatttgccc aacctaaagc gatcatctga agcgtaccgc
    21961 caagtacgat accagcttcg aggttaccgg taactaaacc gatcaatgta catgccacta
    22021 gtggttgatg aaattgaaat tcatcaagaa taccttccat accagctaga aaggcaacta
    22081 atacgactaa aattattgaa ataatagaca tgataagcct cctataaatt ttaatattat
    22141 ttattacgct tttgctaatt cagcttttgc ttttttcaaa atatcatcca tgttatcgcg
    22201 tgaatcatta ggtactttac gtacatcgaa tttcacacct ttttgttcaa gcttctcaaa
    22261 cgcttcaacg tcttcttgtc ccatagacaa tactttactt acaacgactt taccgaccga
    22321 atgcgccatt gacccaacat ttaattcttt gatgtctacg ccaccttcaa cagctgtcaa
    22381 tacgtcttca ggattttcaa ataaaagcaa tgctttggta tttccaaaac gagggtcttt
    22441 tgctacttcg atcattttgc taataggaat aacatttgct tttacacctg gaggagcagc
    22501 ttgttcgatt agtctcttac gcagatcatc ctttgatact gcatctgaaa caacgataat
    22561 acggtttggt tgcactgctt ttgtccaagc tgtcgcaact tgtccatgta acagacgtga
    22621 gtcaacacgt gctaatacat atttgatttt tccgtctccg accacagttc cttctggaag
    22681 tgctccttta ggttgtgcgt cttcaactgc tgctttcggt gcttctgccg gttctaattc
    22741 ttccggtttt acttttacac cgtcttttgc tgtttcaatg atgtgtgctg caatttcatg
    22801 agcagattcc attgaaaagc gtgatgcgta ggcttcaatc aacattggta aattcaaacc
    22861 gctgacgatt gcccatttat ctttgtgctc ttcgaataat gtgtttgatt gattgaacgg
    22921 tgttccgccc cataaatcaa ctaagaataa cacttcgtct tgattatcaa aagaagcaac
    22981 cgcttcttcc aatttggctc tcaagtcatc tggaccttca cttggtttca aaatcacggc
    23041 tttgacgttt tcttgttcgc caaagatcat tgaaccggat tgtaagattc cttcagcaaa
    23101 ttgtccatga cttgctagga taatccctac catatttata cctcctattt acttttgttg
    23161 taacaaattc aaaaatatta ataattgcta accattacaa cagaattatt tgaccttttc
    23221 caacaaccat tcttgctaca cctttttgat tttcgctagt agttccatca aaaggatttt
    23281 cctgtcagca ggtacttttc gaatctctaa ctccacttct tgactgttca agtaacgaaa
    23341 ggaatcaata tcttgttgat ttactgaaac aaaattggta atcattgatt tgccttcagt
    23401 aaaactcata ccgccgatat tcaacgactt caactgtaca ccgcagcgga caaccgcttc
    23461 aacatcttgg ggattggtga ataaaagcat cacttttgct cgtgataata atccactatg
    23521 atagatttga atcattttct gcacggttat tacatttgct gtgatccccg gcggtgcagc
    23581 ttcttttagt agaaactttc tcaatttatc ttgagcgact acatcgctga tcacaatgat
    23641 acgttcaaca cctgtttgct tcgaccaaac tgttgcgact tgcccgtgga ttaaacgatc
    23701 atctatacgt gccaatcgaa tatccatctg caaaactcct tcttcctcac tacttcacag
    23761 aaacccctat catttctgtg tgaacaacca cctcctagca cacactacta taagcaaaaa
    23821 gcgtgccaac ttttttacga tacactaatt gaatttaaag gattcttaac caatacacaa
    23881 aaacgataca ctataaaagt gtatcgtttt tgtgtattca tggcacagtg tattcttttt
    23941 tatctaactg attatggact agacgtagca gataatacat ttcttctatc ggcactttta
    24001 cttttatctg atcttccaac agaacaactg aggctgccaa ttggctataa agaggatcat
    24061 tcgttgtatt tgtcaattcg ttttcttcca cagtcaatgg ttcattccga ataatacgtt
    24121 cgatcattcc tgctgtgtgc atcactaggt tgattttgaa tccatattct tcatcgccga
    24181 ttcccgtcgt ctgagagatt tgtgtagaaa attgccacat tggatcgatt aacttactac
    24241 cattaataaa gatgaagtta tcagagataa actcaacaca cgtattttta gctgactctt
    24301 catctaggga gatgaattca gtttcctcca attctgattc catcagcaac tgatccaaaa
    24361 tgacttcgat attttgatca ataaaacgtt ctaaaggaat aaatggggct tggatattca
    24421 aatcagtaat cccagtagta gctatgatct gatattcttt ttggataact ggcagttctt
    24481 ctttcacttc cacaatcgac aaagctagaa cttctaattc tgtttcttga cgcttgttta
    24541 ccgctcgttc gatcaattct ttgattcttt gtgctgtacc ttcgccagat gcgcagatag
    24601 ccaaaacagc ttttttctta tgaataggtt ttgcttcctg aacagattct acagaagata
    24661 tttctgcgta acctcgaaaa tttttcaatg attcatgcaa catatctaga tctgtcccta
    24721 atactgagac ctttcgtaca gtttccaaaa ctagagaagt tgttaccata tcaacagtac
    24781 gtactggaat tccagtgtct ctttgaattt gcgaattaaa cgacgcaagg gaccccatat
    24841 ctactaagag tatgaccccg cttccttcat ctacttcttg tacattgcgt tcaattcggt
    24901 ccaatgctgt tttcggatcc atatccaaag gcatgtctac tgctcgtacg ttctctgcat
    24961 ctaacaactg ttggacaact tggaccatac tgcttgctgt gctgtttccg tgagctgcaa
    25021 cgactacacc gattctgccg cttgcttgat ttgcccgtaa cgagacgagt agaacggtca
    25081 agtaatagtc ttcactttct ggaatctcca cttgaaaata cgtttcgata taactacgca
    25141 cttctttcgc aacagcatat tcatctggaa aatcaaaagc catctcacgg atgttatcat
    25201 tcgtatgccg ttcttctcct aagttgattt ttttcaaaaa agaactgata tgcaagctca
    25261 tagcataaat aaagttttgc tgaaactcat atggtaaaga tcttttgacc attacataaa
    25321 tctgattggt cacttctatg attttcggat ctacaaattc tgctaatttg ctgtctgcat
    25381 taaaagaaaa accatggtct ttatagaaag acttcagatg gatgttgata tctgtagaaa
    25441 tgaaatgatt gattgcttct tgatccaaac catccgactt cagtaaagct gctttatctc
    25501 cgatgatatc atatagatta taaggtaact cataggagtc cgattggatt ttcatgatcg
    25561 tatcatttgg tgctacagtt atctttggct ccaatatttt agaaagctct gccattgcac
    25621 tgcggttgct tgctaattga atcaatccgc tacgaatccc ttctgtcaaa tcatcgattg
    25681 tgatcgagat ttcgggtgat tgcatatgat ttagaaatcc tcgtgcacat actaactgga
    25741 tatttgactt caattgtcct atatttccat aggtcacact gccgatcaat gcttttgcta
    25801 catcttctgt caaagaaatc tttcgctgcg tgcggtttgc ttcgtgggca atcatgattt
    25861 ttactaaatc gactttctca tttgcaggac gtttttcaaa agccggaagt ttgatattga
    25921 ttgggatacg ccgaacaaaa gtttctaata gagaagaacc tgggtcttct gtggtcgcac
    25981 ctacgatccg tacatttgct tcatgggatt tcgttgtttc cccaagacga ctgtaagttc
    26041 catgatccat aaagtaaaag atcatttctt gaccttcagg cggcaaccgg tgaatctcat
    26101 ccaaaaacag cataccgcca tccgcttgat cgataatccc cattttttct tcatctgcgc
    26161 ctgtaaaagc accttttgcg tacccaaata aatgactcat caaaagctca gggttattgg
    26221 cataatcagc acaattgaaa acaatcagtt ccgtttcctc atctattaca tgatgcgctt
    26281 ttgcaaaatg atacatcgcg tgtgcaaaat aagtctttcc tgatccggta ggtcctgtaa
    26341 tcaaacaatt taatcctctt ggcggataca aaatcgcagc ttttgcctgt tccactgaat
    26401 ttttcatgct gccgttagct ccaatgattt ttgcaaagat atccttagta tctatttgga
    26461 gtgtattttt ttcttcagag accggcgttt ctttgtaact gactacatgt tttgtaactg
    26521 gctttacgtg cgtaacgagt gtttgatata tgtaacgtac tggtcgtccg tctgtcttta
    26581 tcagttttcc ttcgcgcact agctgattca gatctttact gctatttgtt cgctgaatcc
    26641 ctaacacttc agcaatttcc tgtgttgtta ctccttgatc atattctgcc tttgttaaat
    26701 gagcggtttt ttctttgaca tacatataaa ttcggtcaat gcgttttgcc aatggtatcg
    26761 ctccttttct atagactatt gtacttaggc ttcaaaacat aatactatgt atttatttct
    26821 gtttcgtcaa ctatttacct gattttgttt tgttgataac acgcggatag atagaaatgt
    26881 tcaatttaaa agaacgaaaa atcccgatac gatttttata atggaaacaa gaaaaagaac
    26941 actgtttacc atataattat ttcactgata gcaaacaata tagcttccat caattgaaaa
    27001 cgatagttat caaaacatcc tgattccaaa ttggcaaaaa aatattaagt gtaaaaagac
    27061 aaagtaaact tcgtagcctc cactacaact tttattttta taaacaaaaa aggctgtgac
    27121 aaaagtcttg tcacaacctc tttaatcata agattcaatt aagcttcttg agctactgga
    27181 taaacagaca cttcaattat actgtttcct gataagctct caattcttct gaacccatga
    27241 tgttagtgct tttcttctat tattaaatat cttggaattt tctattcttt ctaaaaagta
    27301 tggtcaaagt atggtcacta ttgatagaat gttaatagta gtagaaagct gatatctagg
    27361 aggaactaat gtgagtaaac aatgtgcaaa gtgcggaggt aaaattggct taacttcata
    27421 taaaatcaaa gataaacaaa ttgtttgcgg caattgtatg aaaaaagctg gttacggtat
    27481 gactactcct tataaaataa taagatcttt gaatttagaa gatttagata aggatccatc
    27541 agaaaaaaga aaattaaaca tatctgatca tactgcacaa aacaatcatg atataggtaa
    27601 tgatagaatg agaaccactg aagagatggt agaattttgt cttaaatacg gctacggtaa
    27661 aggaatgact aaaaaatgga caactcatca ttttaactta atatcagatc aacttaacag
    27721 tgacgaatac gtaatcttct gctttgtagg attgcataat tatatctcat caactaagca
    27781 tgacaataat tatgcatacg cattaactaa taaacgttta attgtagctc aacaaaaaat
    27841 gataggcaat aatgtacaaa gtattatatt agacaattta aataatatta gtaaaaaaag
    27901 aggagtgctg ttgggtacat taactattga tactcttggt aaagtattga atgttgcagt
    27961 tgataaagat actgttgata ggataagtga ttctttaaac gaaattattt ataatttaaa
    28021 aagaagaaat atttctgcta caaattccaa tacatcaaca atttcctcag cttcagaaat
    28081 aagaaaatat aaagaactat tggatgatgg aatcattaca gaagaagaat ttaataaaaa
    28141 gaaacaagaa ttattagatt tataatatct atcaaagaag atgggcattt aatttagctc
    28201 atctttttct atttacaata caaacatttg ttcgtatact ctttttgagg tgaactttat
    28261 atgttaatgg aaggaaaaac acaattatgg tttaaattcg atccttcgaa tagatttgtc
    28321 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
    28381 ttaatcaatc tctactattc taataagaac tatttcaaaa ttcctgctgc gaaaactaga
    28441 atgaagaagg gtgtatactt tttgtttgat atcgtgactg acgtgccgga cgcacgtagc
    28501 gatcatcggc gttatgacta tataaagtat actttcgttg atccagaaag atacaaagat
    28561 taaataaaaa aataacaaac aaactcgctt gaaattcatt aggtgagttt gtttattatt
    28621 ttttctgctt ctcgattcat cagcgccata tctgttttcg taatggtttc attaggaata
    28681 tagtcagcac attttctttt ctctaacatt ctatacccac tattatgtat tcctaaggta
    28741 ttcaattcct taactgttga aaacaaattc caaacggttt ggtggcttcc ttttccgcta
    28801 cttccattat aaataatatc ataattttct ttcaaatact catcagattt tttaaaagca
    28861 ctgtagtatg ctcttgaaac agcagttctt ctataagatt ctttagaatt atatttatca
    28921 tctgattcaa tttgtttgga aatagataaa tattcattaa agtcaaacat tttattcccc
    28981 caaagttgct actacagatt tccccatata tttatagtca gataatacat tatcgagagt
    29041 atccaaaaca tcgtaatctg aggaaaacaa ttcagatgtt gttttgataa caaagaaata
    29101 caagtttaaa tcttcatcat attttatatt gatttcagct tttcttttta aaaccgtatt
    29161 tatcacatca aaaattttgc catcattact tgaaattgtg ttaatctcat tttttgtagt
    29221 aattacaagt tgattatcat ttttagtttt cggagtagaa cttgcatcca caacaactgg
    29281 agatgctaga attaccgatg cgcaagcaaa aatcgcagca aaatttatta agctaccact
    29341 ccgatgtgta tttgatggta ctatatcaag catccgtatc gtctccttaa tttttttcca
    29401 ctggtttgtt gaccttgata tcaaagattt gccttccaat ctcagttcca ttaaatttaa
    29461 caacagcata atgctctcct gcttcttcaa ccaagacatt tctggcgttt atattaaagt
    29521 ttatatcgtt aacttgagga ggcaagctta ccgatacttc acccgtagaa aaaatttgtt
    29581 tttcattctt gtttaaaact tcaatttcta ctatttttat cttttctgta tcctctatct
    29641 cagacaccac agctgttaca gaaaatgata aagcagtagg aattaaaggc gcattaatag
    29701 aaagagctgg attaattact ttattaataa cattagtgcc aaattgctga ctttctacat
    29761 cttctgaaat tattaattgt actctataag acattgattt ctcctaattt ttacatttat
    29821 ttaataaatt tatgtttcac ttttgctagt caatttagtt taacaatagt aacaaagctt
    29881 tgtcaacgga aatattagct aattttgtaa gcatatttat aagtatattt gtaagcatat
    29941 ctgacattta atcagtagta aaagtgcctc tatataagga acaaactgtt tttttactat
    30001 ctaaattcgt atacagtttt actaacaaat tagctaacaa tattagcaac tatatcaaca
    30061 actatatgta cttcccctca a
//
LOCUS       DACCZY010000023        30035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCZY010000023 DACCZY010000000
VERSION     DACCZY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.023"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..548)
                     /locus_tag="IVZ67_000822"
     CDS             complement(<1..548)
                     /locus_tag="IVZ67_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8164920.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKI"
     gene            complement(625..1155)
                     /locus_tag="IVZ67_000823"
     CDS             complement(625..1155)
                     /locus_tag="IVZ67_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8164921.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1159..2019)
                     /gene="rlmB"
                     /locus_tag="IVZ67_000824"
     CDS             complement(1159..2019)
                     /gene="rlmB"
                     /locus_tag="IVZ67_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8164922.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2012..2407)
                     /locus_tag="IVZ67_000825"
     CDS             complement(2012..2407)
                     /locus_tag="IVZ67_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8164923.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2404..3816)
                     /locus_tag="IVZ67_000826"
     CDS             complement(2404..3816)
                     /locus_tag="IVZ67_000826"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8164924.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3813..4349)
                     /gene="cysE"
                     /locus_tag="IVZ67_000827"
     CDS             complement(3813..4349)
                     /gene="cysE"
                     /locus_tag="IVZ67_000827"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8164925.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4671..6131)
                     /locus_tag="IVZ67_000828"
     CDS             complement(4671..6131)
                     /locus_tag="IVZ67_000828"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8164926.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6301..7458)
                     /locus_tag="IVZ67_000829"
     CDS             complement(6301..7458)
                     /locus_tag="IVZ67_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8164927.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7492..8862)
                     /gene="radA"
                     /locus_tag="IVZ67_000830"
     CDS             complement(7492..8862)
                     /gene="radA"
                     /locus_tag="IVZ67_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8164928.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8925..9452)
                     /locus_tag="IVZ67_000831"
     CDS             complement(8925..9452)
                     /locus_tag="IVZ67_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8164929.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9682..10488
                     /gene="proB"
                     /locus_tag="IVZ67_000832"
     CDS             9682..10488
                     /gene="proB"
                     /locus_tag="IVZ67_000832"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8164930.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10485..11732
                     /locus_tag="IVZ67_000833"
     CDS             10485..11732
                     /locus_tag="IVZ67_000833"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8164931.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11808..12152
                     /locus_tag="IVZ67_000834"
     CDS             11808..12152
                     /locus_tag="IVZ67_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164932.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12269..12595)
                     /locus_tag="IVZ67_000835"
     CDS             complement(12269..12595)
                     /locus_tag="IVZ67_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164933.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13210..14412)
                     /locus_tag="IVZ67_000836"
     CDS             complement(13210..14412)
                     /locus_tag="IVZ67_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8164934.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14529..15044
                     /locus_tag="IVZ67_000837"
     CDS             14529..15044
                     /locus_tag="IVZ67_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8164935.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15243..15689)
                     /locus_tag="IVZ67_000838"
     CDS             complement(15243..15689)
                     /locus_tag="IVZ67_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8164936.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15736..17214)
                     /gene="msr"
                     /locus_tag="IVZ67_000839"
     CDS             complement(15736..17214)
                     /gene="msr"
                     /locus_tag="IVZ67_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8164937.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17568..18197)
                     /locus_tag="IVZ67_000840"
     CDS             complement(17568..18197)
                     /locus_tag="IVZ67_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8164938.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18181..18645)
                     /locus_tag="IVZ67_000841"
     CDS             complement(18181..18645)
                     /locus_tag="IVZ67_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8164939.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19132..20400)
                     /locus_tag="IVZ67_000842"
     CDS             complement(19132..20400)
                     /locus_tag="IVZ67_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8164940.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20631..21368)
                     /locus_tag="IVZ67_000843"
     CDS             complement(20631..21368)
                     /locus_tag="IVZ67_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8164941.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21435..22004)
                     /locus_tag="IVZ67_000844"
     CDS             complement(21435..22004)
                     /locus_tag="IVZ67_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8164942.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22132..23004
                     /locus_tag="IVZ67_000845"
     CDS             22132..23004
                     /locus_tag="IVZ67_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8164943.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23346..24218
                     /locus_tag="IVZ67_000846"
     CDS             23346..24218
                     /locus_tag="IVZ67_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8164944.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24215..26173
                     /locus_tag="IVZ67_000847"
     CDS             24215..26173
                     /locus_tag="IVZ67_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8164945.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26505..27356)
                     /locus_tag="IVZ67_000848"
     CDS             complement(26505..27356)
                     /locus_tag="IVZ67_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8164946.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27479..27946
                     /locus_tag="IVZ67_000849"
     CDS             27479..27946
                     /locus_tag="IVZ67_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8164947.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28209..28691)
                     /locus_tag="IVZ67_000850"
     CDS             complement(28209..28691)
                     /locus_tag="IVZ67_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8164948.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28791..29654)
                     /locus_tag="IVZ67_000851"
     CDS             complement(28791..29654)
                     /locus_tag="IVZ67_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8164949.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8564 a         6363 c         5448 g         9660 t
ORIGIN      
        1 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
       61 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
      121 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
      181 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
      241 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
      301 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
      361 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
      421 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
      481 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
      541 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
      601 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
      661 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
      721 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
      781 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
      841 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
      901 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
      961 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
     1021 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
     1081 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
     1141 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
     1201 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
     1261 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
     1321 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
     1381 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
     1441 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
     1501 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
     1561 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
     1621 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
     1681 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
     1741 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
     1801 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
     1861 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
     1921 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
     1981 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
     2041 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
     2101 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
     2161 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
     2221 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
     2281 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
     2341 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
     2401 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
     2461 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
     2521 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
     2581 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
     2641 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
     2701 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
     2761 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
     2821 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
     2881 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
     2941 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
     3001 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
     3061 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
     3121 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
     3181 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
     3241 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
     3301 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
     3361 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
     3421 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
     3481 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
     3541 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
     3601 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
     3661 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
     3721 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
     3781 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
     3841 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
     3901 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
     3961 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
     4021 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
     4081 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
     4141 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
     4201 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
     4261 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
     4321 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
     4381 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     4441 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
     4501 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
     4561 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
     4621 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
     4681 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
     4741 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
     4801 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
     4861 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
     4921 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
     4981 agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc
     5041 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
     5101 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
     5161 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
     5221 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
     5281 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
     5341 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
     5401 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
     5461 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
     5521 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
     5581 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
     5641 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
     5701 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
     5761 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
     5821 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
     5881 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
     5941 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
     6001 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
     6061 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
     6121 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
     6181 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
     6241 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
     6301 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
     6361 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
     6421 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
     6481 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
     6541 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
     6601 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
     6661 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
     6721 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
     6781 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
     6841 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
     6901 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
     6961 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
     7021 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
     7081 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
     7141 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
     7201 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
     7261 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
     7321 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
     7381 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
     7441 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
     7501 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
     7561 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
     7621 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
     7681 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
     7741 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
     7801 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
     7861 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
     7921 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
     7981 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
     8041 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
     8101 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
     8161 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
     8221 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
     8281 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
     8341 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
     8401 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
     8461 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
     8521 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
     8581 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
     8641 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
     8701 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
     8761 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
     8821 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
     8881 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
     8941 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
     9001 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
     9061 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
     9121 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
     9181 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
     9241 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
     9301 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
     9361 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
     9421 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
     9481 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
     9541 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
     9601 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
     9661 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
     9721 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
     9781 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
     9841 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
     9901 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
     9961 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    10021 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    10081 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    10141 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    10201 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    10261 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    10321 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    10381 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    10441 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    10501 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    10561 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    10621 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    10681 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    10741 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    10801 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    10861 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    10921 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    10981 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    11041 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    11101 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    11161 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    11221 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    11281 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    11341 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    11401 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    11461 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    11521 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    11581 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    11641 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    11701 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    11761 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    11821 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    11881 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    11941 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    12001 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    12061 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    12121 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    12181 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    12241 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    12301 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    12361 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    12421 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    12481 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    12541 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    12601 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    12661 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    12721 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    12781 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    12841 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    12901 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    12961 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    13021 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    13081 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    13141 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    13201 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    13261 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    13321 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    13381 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    13441 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    13501 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    13561 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    13621 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    13681 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    13741 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    13801 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    13861 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    13921 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    13981 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    14041 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    14101 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    14161 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    14221 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    14281 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    14341 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    14401 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    14461 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    14521 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    14581 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    14641 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    14701 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    14761 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    14821 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    14881 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    14941 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    15001 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    15061 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    15121 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    15181 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    15241 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    15301 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    15361 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    15421 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    15481 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    15541 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    15601 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    15661 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    15721 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    15781 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    15841 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    15901 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    15961 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    16021 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    16081 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    16141 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    16201 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    16261 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    16321 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    16381 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    16441 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    16501 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    16561 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    16621 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    16681 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    16741 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    16801 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    16861 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    16921 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    16981 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    17041 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    17101 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    17161 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    17221 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    17281 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    17341 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    17401 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    17461 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    17521 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    17581 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    17641 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    17701 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    17761 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    17821 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    17881 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    17941 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    18001 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    18061 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    18121 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    18181 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    18241 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    18301 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    18361 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    18421 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    18481 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    18541 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    18601 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    18661 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    18721 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    18781 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    18841 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    18901 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    18961 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    19021 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    19081 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    19141 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    19201 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    19261 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    19321 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    19381 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    19441 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    19501 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    19561 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    19621 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    19681 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    19741 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    19801 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    19861 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    19921 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    19981 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    20041 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    20101 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    20161 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    20221 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    20281 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    20341 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    20401 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    20461 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    20521 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    20581 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    20641 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    20701 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    20761 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    20821 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    20881 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    20941 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    21001 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    21061 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    21121 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    21181 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    21241 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    21301 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    21361 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    21421 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    21481 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    21541 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    21601 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    21661 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    21721 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    21781 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    21841 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    21901 aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact
    21961 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    22021 atccttactt tataacggac ggcttatgat gtctaatact tataaaacat acttaactat
    22081 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    22141 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    22201 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    22261 ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat
    22321 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    22381 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    22441 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    22501 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    22561 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    22621 cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg
    22681 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    22741 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    22801 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    22861 atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca
    22921 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    22981 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    23041 acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct
    23101 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    23161 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    23221 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    23281 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    23341 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    23401 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    23461 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    23521 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    23581 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    23641 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    23701 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    23761 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    23821 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    23881 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    23941 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    24001 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    24061 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    24121 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    24181 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    24241 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    24301 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    24361 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    24421 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    24481 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    24541 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    24601 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    24661 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    24721 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    24781 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    24841 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    24901 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    24961 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    25021 agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac
    25081 agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    25141 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    25201 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    25261 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    25321 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    25381 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    25441 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    25501 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    25561 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    25621 agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt
    25681 ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa
    25741 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    25801 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    25861 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    25921 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    25981 aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    26041 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    26101 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    26161 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    26221 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    26281 cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct
    26341 tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt
    26401 ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
    26461 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    26521 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    26581 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    26641 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    26701 tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    26761 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    26821 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    26881 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    26941 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    27001 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    27061 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    27121 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    27181 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    27241 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    27301 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    27361 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    27421 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    27481 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    27541 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    27601 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    27661 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    27721 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    27781 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    27841 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    27901 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    27961 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    28021 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    28081 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    28141 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    28201 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    28261 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    28321 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    28381 gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt
    28441 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    28501 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    28561 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    28621 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    28681 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    28741 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    28801 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    28861 aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag
    28921 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    28981 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    29041 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca
    29101 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    29161 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    29221 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    29281 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    29341 atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    29401 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    29461 aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    29521 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    29581 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    29641 agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa
    29701 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    29761 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    29821 ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa
    29881 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    29941 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    30001 tcaattggaa caattattta tttctaacaa aatca
//
LOCUS       DACCZY010000024        29917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCZY010000024 DACCZY010000000
VERSION     DACCZY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.024"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..708)
                     /locus_tag="IVZ67_000852"
     CDS             complement(<1..708)
                     /locus_tag="IVZ67_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8164950.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLD"
     gene            complement(820..1851)
                     /locus_tag="IVZ67_000853"
                     /pseudo
     CDS             complement(820..1851)
                     /locus_tag="IVZ67_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1933..2622)
                     /locus_tag="IVZ67_000854"
     CDS             complement(1933..2622)
                     /locus_tag="IVZ67_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8164951.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2728..3096)
                     /gene="rplL"
                     /locus_tag="IVZ67_000855"
     CDS             complement(2728..3096)
                     /gene="rplL"
                     /locus_tag="IVZ67_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8164952.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(3149..3652)
                     /locus_tag="IVZ67_000856"
     CDS             complement(3149..3652)
                     /locus_tag="IVZ67_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8164953.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(4062..4751)
                     /gene="rplA"
                     /locus_tag="IVZ67_000857"
     CDS             complement(4062..4751)
                     /gene="rplA"
                     /locus_tag="IVZ67_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8164954.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4855..5277)
                     /gene="rplK"
                     /locus_tag="IVZ67_000858"
     CDS             complement(4855..5277)
                     /gene="rplK"
                     /locus_tag="IVZ67_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8164955.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5620..6288
                     /gene="sdaAB"
                     /locus_tag="IVZ67_000859"
     CDS             5620..6288
                     /gene="sdaAB"
                     /locus_tag="IVZ67_000859"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8164956.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            6301..7173
                     /gene="sdaAA"
                     /locus_tag="IVZ67_000860"
     CDS             6301..7173
                     /gene="sdaAA"
                     /locus_tag="IVZ67_000860"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8164957.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(7264..8283)
                     /locus_tag="IVZ67_000861"
     CDS             complement(7264..8283)
                     /locus_tag="IVZ67_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8164958.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(8456..9343)
                     /locus_tag="IVZ67_000862"
     CDS             complement(8456..9343)
                     /locus_tag="IVZ67_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8164959.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(9420..9965)
                     /gene="nusG"
                     /locus_tag="IVZ67_000863"
     CDS             complement(9420..9965)
                     /gene="nusG"
                     /locus_tag="IVZ67_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8164960.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(10066..10236)
                     /gene="secE"
                     /locus_tag="IVZ67_000864"
     CDS             complement(10066..10236)
                     /gene="secE"
                     /locus_tag="IVZ67_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8164961.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(10260..10412)
                     /gene="rpmG"
                     /locus_tag="IVZ67_000865"
     CDS             complement(10260..10412)
                     /gene="rpmG"
                     /locus_tag="IVZ67_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8164962.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            10574..11218
                     /locus_tag="IVZ67_000866"
     CDS             10574..11218
                     /locus_tag="IVZ67_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8164963.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(11274..11777)
                     /locus_tag="IVZ67_000867"
     CDS             complement(11274..11777)
                     /locus_tag="IVZ67_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8164964.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11790..11836)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12374..13339
                     /locus_tag="IVZ67_000868"
     CDS             12374..13339
                     /locus_tag="IVZ67_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8164965.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(13438..14610)
                     /locus_tag="IVZ67_000869"
     CDS             complement(13438..14610)
                     /locus_tag="IVZ67_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164966.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14597..15742)
                     /locus_tag="IVZ67_000870"
     CDS             complement(14597..15742)
                     /locus_tag="IVZ67_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8164967.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15756..16688)
                     /locus_tag="IVZ67_000871"
     CDS             complement(15756..16688)
                     /locus_tag="IVZ67_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8164968.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16805..17884
                     /locus_tag="IVZ67_000872"
     CDS             16805..17884
                     /locus_tag="IVZ67_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8164969.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17881..18499
                     /locus_tag="IVZ67_000873"
                     /pseudo
     CDS             17881..18499
                     /locus_tag="IVZ67_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(18609..18836)
                     /locus_tag="IVZ67_000874"
     CDS             complement(18609..18836)
                     /locus_tag="IVZ67_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8164970.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18939..21527)
                     /locus_tag="IVZ67_000875"
     CDS             complement(18939..21527)
                     /locus_tag="IVZ67_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8164971.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(21547..22206)
                     /locus_tag="IVZ67_000876"
     CDS             complement(21547..22206)
                     /locus_tag="IVZ67_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8164972.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(22255..22758)
                     /gene="trmL"
                     /locus_tag="IVZ67_000877"
     CDS             complement(22255..22758)
                     /gene="trmL"
                     /locus_tag="IVZ67_000877"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8164973.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22979..23845)
                     /locus_tag="IVZ67_000878"
     CDS             complement(22979..23845)
                     /locus_tag="IVZ67_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8164974.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23954..24595
                     /locus_tag="IVZ67_000879"
     CDS             23954..24595
                     /locus_tag="IVZ67_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8164975.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24657..26018)
                     /gene="mgtE"
                     /locus_tag="IVZ67_000880"
     CDS             complement(24657..26018)
                     /gene="mgtE"
                     /locus_tag="IVZ67_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8164976.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(26036..26935)
                     /locus_tag="IVZ67_000881"
     CDS             complement(26036..26935)
                     /locus_tag="IVZ67_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8164977.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26937..27734)
                     /locus_tag="IVZ67_000882"
     CDS             complement(26937..27734)
                     /locus_tag="IVZ67_000882"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8164978.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27712..28416)
                     /locus_tag="IVZ67_000883"
     CDS             complement(27712..28416)
                     /locus_tag="IVZ67_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8164979.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            28547..29122
                     /locus_tag="IVZ67_000884"
     CDS             28547..29122
                     /locus_tag="IVZ67_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8164980.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            29240..29917
                     /locus_tag="IVZ67_000885"
     CDS             29240..29917
                     /locus_tag="IVZ67_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8164981.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8797 a         6305 c         5383 g         9432 t
ORIGIN      
        1 gtctagtttt tctgatttat ccatgttttt acgtggcgga acggatatag taatgccctt
       61 aagggcaagc ttttcatgga ttttatggct aatatatcct ttgtccccta ggatattggg
      121 aagattcgct tcttccgata aagtctctaa cactttcacg tcatggacac ctggattcgt
      181 gattgagtaa gtaatcggaa agccagtctt agtaacgatc atatgaattt taagaccata
      241 aaaataagat ttttttgttg agttatagcc aactttggct atttgattca atagttttgc
      301 ttgtctattt ctaactggtt tacataatgg actaggaaaa ctatctataa ttccgactag
      361 ttcacctttg gtgagcttct tgatgaaaaa gtatcgaata atcttgatag tgtaagctaa
      421 gtttgaactt aagcgagtga atcgactcct actaggaaag tcaccattag gaaataagac
      481 ggaacaaacc gctctatacg tggctctttg gctagtatag ccattaatta tgccccatat
      541 aacacaagca ataatcactg tatcgcgttg cttcagttga tcagtatttc ttcggttttt
      601 aatcttatct ggtacacaat tgtggtatat gttagaaaca gttctcataa tttccttgaa
      661 tgttataaaa gtatctgtat aatgttcagt atattttgat gaacgcatat gtagtcctca
      721 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
      781 acattttcct ctaggaaaaa attaactagc accacgggta aatacagtat tatttcggtt
      841 gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac caaattgatt
      901 cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc cgccaaccaa
      961 taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt ttctttcaga
     1021 ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca aaccaaaaat
     1081 cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga tataaaaaat
     1141 caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga ctgccgcttc
     1201 tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat caaggtatga
     1261 aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca gaagtgtaag
     1321 aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa gactggcaac
     1381 aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc
     1441 catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg
     1501 cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg taaacgaagg
     1561 cgcaaaaaaa gcggccaaca gagagacggc agataacgct accattcgaa tgatcaattt
     1621 tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg catagcctaa
     1681 ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac tagctgctac
     1741 ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac aagtgattga
     1801 taatagtaaa ataagcgaag ttgttaaagg tgttttttca ttactcttca ttgatttccc
     1861 ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa atacaactat
     1921 tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat ctttataaat
     1981 gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg cagttttgtt
     2041 atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag ggatgactcc
     2101 aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg cagcaaaatt
     2161 gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta tttttggatg
     2221 tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat ctaaaaattt
     2281 taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca aaatgttgat
     2341 gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta ctgaagtagg
     2401 aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg atgcaaaagt
     2461 cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat ttaaaaataa
     2521 ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta tcagaatgat
     2581 tcccaacgag atcaacatca atcggattat tttctttttc atatttcctc ctgaaaaaaa
     2641 tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag
     2701 gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc
     2761 aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg
     2821 agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc
     2881 tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg
     2941 ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac
     3001 gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt
     3061 taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt
     3121 cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca
     3181 ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag
     3241 atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga
     3301 ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt
     3361 ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag
     3421 tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat
     3481 ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt
     3541 catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt
     3601 cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt
     3661 ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata
     3721 gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact
     3781 cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt
     3841 cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact
     3901 attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc
     3961 ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag
     4021 aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca
     4081 acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta
     4141 gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat
     4201 ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca
     4261 cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact
     4321 gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct
     4381 acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg
     4441 attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt
     4501 gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat
     4561 acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca
     4621 acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg
     4681 gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc
     4741 tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt
     4801 ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc
     4861 tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt
     4921 agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac
     4981 tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt
     5041 caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac
     5101 agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt
     5161 tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg
     5221 agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag
     5281 acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt
     5341 ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta
     5401 tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata
     5461 aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta
     5521 cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct
     5581 tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga
     5641 agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga
     5701 gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat
     5761 atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta
     5821 gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg
     5881 gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca
     5941 aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg
     6001 atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg
     6061 ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact
     6121 agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa
     6181 ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa
     6241 agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa
     6301 atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa
     6361 ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc
     6421 atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc
     6481 tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca
     6541 gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa
     6601 gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt
     6661 cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa
     6721 ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt
     6781 gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg
     6841 gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa
     6901 aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa
     6961 cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc
     7021 gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg
     7081 ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga
     7141 cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag
     7201 agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt
     7261 ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc ctgcaagttg
     7321 catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc
     7381 acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc
     7441 cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg
     7501 tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctag
     7561 ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac
     7621 tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat
     7681 tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc
     7741 ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg
     7801 taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc
     7861 tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt
     7921 gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc
     7981 ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc
     8041 agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga
     8101 tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc
     8161 tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt
     8221 ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc
     8281 catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc
     8341 ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa
     8401 atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt
     8461 ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat
     8521 gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca
     8581 acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat
     8641 ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct
     8701 ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag
     8761 actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa
     8821 cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc
     8881 ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa
     8941 gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata
     9001 cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat
     9061 ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca
     9121 acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag
     9181 tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag
     9241 tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg
     9301 ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca
     9361 tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat
     9421 taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata
     9481 tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat
     9541 ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt
     9601 tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt
     9661 gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg
     9721 acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt
     9781 ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca
     9841 acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct
     9901 tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat
     9961 tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt
    10021 tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca
    10081 gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga
    10141 agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg
    10201 tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt
    10261 tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc
    10321 aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg
    10381 gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag
    10441 acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat
    10501 ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat
    10561 tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt
    10621 tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg
    10681 tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat
    10741 ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa
    10801 cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga
    10861 gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag
    10921 tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac
    10981 aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca
    11041 cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac
    11101 attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt
    11161 agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc
    11221 aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca
    11281 cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg
    11341 acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt
    11401 ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact
    11461 aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc
    11521 aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag
    11581 acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc
    11641 aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt
    11701 aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg
    11761 cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt
    11821 gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat
    11881 ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc
    11941 gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa
    12001 gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa
    12061 tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg
    12121 ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggcttttttg
    12181 tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat aaccaagaat
    12241 taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag
    12301 cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt ttattgaata
    12361 gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg
    12421 aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta
    12481 tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa
    12541 agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt
    12601 gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga
    12661 agtagaattc ccagttgctt atattccagc aggttctgga aatgattttg ctcgaggggc
    12721 aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga
    12781 aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa
    12841 taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa
    12901 aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc
    12961 tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt
    13021 ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat
    13081 tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa
    13141 tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa
    13201 atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg
    13261 tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa
    13321 acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt
    13381 attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta
    13441 attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat
    13501 cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc
    13561 aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata
    13621 tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa
    13681 caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc
    13741 tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa
    13801 gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag
    13861 aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt
    13921 tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc
    13981 tttgcgatta aacgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc
    14041 ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc
    14101 cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc
    14161 atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg
    14221 ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcgccat
    14281 ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg
    14341 attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt
    14401 ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat
    14461 caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa
    14521 gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag
    14581 caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc
    14641 ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa aaactagcca
    14701 aatcagtgta gaaataaagt ttatacaaac tttcgctgat caagttgacg agatttaatt
    14761 gaccaacaat cggaatatga agatcaatcc aatacttcac ttgttccgac cccatcatac
    14821 cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat gctatccttt tgcaccagat
    14881 tcatttttgg tatcaaatta ccaaccaatg tacctaacaa aatcgcacac aacgacccca
    14941 gagcggtaac aatcatcaac cattgccaac gttcaccaaa gtctacttga tagataaaac
    15001 gaaagacagc taacataata taaatctctg tcaaaacaat cgtaaagctt gctgccaaat
    15061 tagcgattga aactaacgat tgctttctag gaatcaagga caagcgaata ccatttgacg
    15121 attgatttgc ttgctgatcc tgtacattgc ggacccccca catcgtcccg tagagaatcg
    15181 ccattccaat cagagtgaag aaataaaaac ttttgaaact catattacga cctgcatgaa
    15241 cttcttgaaa agatacttct tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct
    15301 ccacttctgc cactttactc gaaattatac caatacgctg taaaaattga tccatgacgt
    15361 ttttcatcaa cgtctgactc attcctgctt gacccacatg gagcgaaaca gatgaaccat
    15421 caatgagata aaaaccagcc aaatcatctg catttagctt ttcattcgct tctacttccg
    15481 atagttcctg tacttcaaat aatggtttat cattgacttc gacttgttct aagactgtgg
    15541 taaaattttc cgaaagcgct gtttgatccg ctttgattac aattccagta ttaatcgttt
    15601 caagattgcc accttgatca atctcgccaa aggccgccat aaacatcaat cctaaaagaa
    15661 ttggaaaggc caatgtccaa aacaacatgg atttatttcg cagcagtatt tttgtgcgat
    15721 atttataaaa atgccaaaac attatttccc ctcctctagt ctctcaaatc cttgccagta
    15781 atctctaaaa agacatcatt caaggacgct tctttggttg ctatttttcc gtaactgatc
    15841 ttttcttctt gtaagattgt aagaatcggc aacaatactg tatttagttc agtagttgtt
    15901 acttggagct gacctttttt ataagcgatt ccatcagaag ctattgttcg ctgaatcgct
    15961 gacaacacat tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
    16021 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
    16081 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
    16141 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
    16201 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
    16261 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
    16321 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
    16381 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
    16441 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
    16501 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
    16561 gtcgatttcc cactgccgtt tggacctaac agacctaaga cttctccttc ttgtacttgc
    16621 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
    16681 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
    16741 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
    16801 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
    16861 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
    16921 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
    16981 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
    17041 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
    17101 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
    17161 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
    17221 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
    17281 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
    17341 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
    17401 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
    17461 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
    17521 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
    17581 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
    17641 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
    17701 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
    17761 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
    17821 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
    17881 atgataacag tagtcattat tgatgatgat ccatttgtga cgaaatcgtt acaaaccatt
    17941 ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcga
    18001 aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa cgggcatcga
    18061 tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt tgacgacatt
    18121 caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa
    18181 acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac aggccgtatt
    18241 tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga cgattgatag
    18301 tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg
    18361 gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg ttcgcaatta
    18421 catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg ctatctattt
    18481 ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc
    18541 atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt tcctcacgat
    18601 tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg cgtgttaggc
    18661 ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg atcgtctcgt
    18721 tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa ctttttaaag
    18781 tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt agccataaag
    18841 taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa agaaaacgct
    18901 aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt tgccggtata
    18961 aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg gttatcgatc
    19021 acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc tttcttttct
    19081 tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg gtttttcgtt
    19141 tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg tggctctttt
    19201 gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata agctgctagt
    19261 ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt caacgtagtc
    19321 atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc ccctaacaaa
    19381 atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg aagcatccgc
    19441 tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg tgatttatgg
    19501 attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata agtgacctca
    19561 tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt tgccaagatg
    19621 atacccgtga tttctcccat atctccattg aatacattca attcgggagt attgactaat
    19681 tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc cttttttcca
    19741 ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat ccctgcaggt
    19801 ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt atttttcgct
    19861 ttttccacaa ttttagagat caagggttca atttgataag catcacttcg gaaataggaa
    19921 cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat ctcatgggca
    19981 agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc cattttggga
    20041 atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga gggaagctga
    20101 tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta
    20161 gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc ttctaattct
    20221 ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg atggatcgta
    20281 ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt tggtgctgca
    20341 agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag tccgttcaac
    20401 tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc tggtcctcct
    20461 gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg cgaaggtcca
    20521 tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc tttttcagga
    20581 taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat cccccattca
    20641 gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt tccttcttca
    20701 acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg gcgacttgtt
    20761 tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt
    20821 tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct cggtgaatct
    20881 gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat tccttctatg
    20941 tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt cttataggcc
    21001 tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc catgatcact
    21061 tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc
    21121 aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat agcatcttct
    21181 cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg
    21241 gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata tctttcgagt
    21301 ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg ataagtctca
    21361 ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg gtaatcagct
    21421 gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa
    21481 atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg aagccgcctc
    21541 cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg tttggttcca
    21601 tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa tcgtcaaact
    21661 actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg tcatccattg
    21721 aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat ctttatgtcg
    21781 ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc
    21841 gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca gattatccgg
    21901 atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc ctagttctct
    21961 tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga taccttctgc
    22021 tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa catattttac
    22081 ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag aatcgccatt
    22141 gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg taaaatatag
    22201 ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa ctgtctaatc
    22261 cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag
    22321 tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga catgctcatc
    22381 attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt ctggcaaacc
    22441 ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact
    22501 atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta aaaatgttaa
    22561 aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat ccaaccctgc
    22621 ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat gaagcggagt
    22681 attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta atggttcaaa
    22741 taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt ctattttctg
    22801 acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt
    22861 tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat aaaaaccaat
    22921 gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc tctactcatt
    22981 aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg tcaattgtaa
    23041 tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc tgccattcta
    23101 aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat tcataggtta
    23161 cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca cgttcattgg
    23221 aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc tttaaataat
    23281 tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt tggaattctt
    23341 ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca
    23401 aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta gctgcgtaga
    23461 ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat tttgaaagtt
    23521 cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct tcaggtaaat
    23581 gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt ctacgccttg
    23641 gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa taaagtgttc
    23701 cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta tcagactttt
    23761 ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc aaaaactggc
    23821 gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc ttaccgttag
    23881 atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag tgaaagataa
    23941 aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta ttcctctagc
    24001 aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac
    24061 cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag atatccccgt
    24121 aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg aattgaaaat
    24181 catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag tatttggttg
    24241 tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg aaacagcaga
    24301 agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc ggcaattcga
    24361 agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc
    24421 tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct atacagatgg
    24481 ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac atgtagctaa
    24541 agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt tataatctta
    24601 taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt gtttttttac
    24661 ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact cgtcaaatcg
    24721 ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag acgatccatt
    24781 agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag catagccatc
    24841 cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg
    24901 atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac aagtcgaaca
    24961 aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc tagcgactgg
    25021 gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa aatactcgct
    25081 tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc taaaaacaac
    25141 agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc attaatttct
    25201 tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc aataacgtcg
    25261 atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt aacaggaaga
    25321 gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc gcctacatga
    25381 actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc attcacgatt
    25441 aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac atagatgtaa
    25501 tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt
    25561 tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg
    25621 taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact taaatattta
    25681 gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt atacatctca
    25741 gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt catatgttcg
    25801 ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag ataggaatag
    25861 atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc atagatatgc
    25921 atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc ttgagtcaac
    25981 tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc tcgccttatt
    26041 cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg aactccagca
    26101 actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct tgtcttttta
    26161 ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag ccaattgcgg
    26221 aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca actaacgcga
    26281 tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct tcatcagaga
    26341 ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata tgtccatgtg
    26401 ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca aacttctttt
    26461 cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc
    26521 cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg taaccttgtt
    26581 ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat tgggcaggaa
    26641 tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca tacacgatat
    26701 cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga atcacaatgg
    26761 ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca ttaactttga
    26821 tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct ttttctttta
    26881 aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac tgcatatcaa
    26941 tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg ataagaagca
    27001 aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc ttttgcgaca
    27061 ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat ttctagccat
    27121 tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt attcagtgaa
    27181 gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc gccgacactt
    27241 ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc tctaaaactt
    27301 tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc ccgtttaatc
    27361 gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata actgatccga
    27421 acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct gtctactagt
    27481 tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt atgcacgccc
    27541 aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa taatgtgccg
    27601 tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat ccctgcttgt
    27661 tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt attatgaacg
    27721 atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa gcgttcgata
    27781 gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt cttggatttc
    27841 ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg cacgctgaag
    27901 tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg aatgctcaat
    27961 cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa ttttcttctc
    28021 accgctgatc agctgtacag gatactcgat caccagatga taagaacggt agccacttgc
    28081 ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc ttttacggat
    28141 gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta acccggcaat
    28201 atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg attttgttac
    28261 aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat gtcgattttg
    28321 ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata cagtttgttc
    28381 atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta cttcattaac
    28441 attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt tatgcattga
    28501 ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga cacaaatgct
    28561 tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca tcgctcacta
    28621 tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata ccgttgatga
    28681 acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc aagctgaata
    28741 tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct ttataacaga
    28801 agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa
    28861 actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc ttacaacaaa
    28921 acgagctgaa ttccctattg aagaaggact tttagctatt gatgaaagct ggggagacaa
    28981 tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa aaattgcttt
    29041 tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca agatccaacg
    29101 tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga gttcattttt
    29161 ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt gtatagaatc
    29221 tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt taggggaata
    29281 ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg ggacaccatc
    29341 aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa ttggggatgt
    29401 catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact tattctcaat
    29461 gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg
    29521 ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat attctcaaga
    29581 acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta aagaagatcg
    29641 tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc gggaaatggg
    29701 catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg attttggtgt
    29761 tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt gtaaaacaac
    29821 agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt ctcagttcca
    29881 ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DACCZY010000025        29572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCZY010000025 DACCZY010000000
VERSION     DACCZY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.025"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            1..270
                     /locus_tag="IVZ67_000886"
                     /pseudo
     CDS             1..270
                     /locus_tag="IVZ67_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            289..603
                     /locus_tag="IVZ67_000887"
     CDS             289..603
                     /locus_tag="IVZ67_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164982.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(656..1429)
                     /locus_tag="IVZ67_000888"
     CDS             complement(656..1429)
                     /locus_tag="IVZ67_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164983.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1534..1779
                     /locus_tag="IVZ67_000889"
     CDS             1534..1779
                     /locus_tag="IVZ67_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8164984.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1935..2786
                     /locus_tag="IVZ67_000890"
     CDS             1935..2786
                     /locus_tag="IVZ67_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164985.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2819..3493
                     /locus_tag="IVZ67_000891"
                     /pseudo
     CDS             2819..3493
                     /locus_tag="IVZ67_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3750..4949
                     /locus_tag="IVZ67_000892"
     CDS             3750..4949
                     /locus_tag="IVZ67_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8164986.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            4971..5183
                     /locus_tag="IVZ67_000893"
     CDS             4971..5183
                     /locus_tag="IVZ67_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8164987.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5258..5734
                     /locus_tag="IVZ67_000894"
     CDS             5258..5734
                     /locus_tag="IVZ67_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP8164988.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5731..7539
                     /locus_tag="IVZ67_000895"
     CDS             5731..7539
                     /locus_tag="IVZ67_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8164989.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7616..7831
                     /locus_tag="IVZ67_000896"
     CDS             7616..7831
                     /locus_tag="IVZ67_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP8164990.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7923..8786
                     /locus_tag="IVZ67_000897"
     CDS             7923..8786
                     /locus_tag="IVZ67_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8164991.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8817..9359
                     /locus_tag="IVZ67_000898"
     CDS             8817..9359
                     /locus_tag="IVZ67_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP8164992.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            9373..9795
                     /locus_tag="IVZ67_000899"
     CDS             9373..9795
                     /locus_tag="IVZ67_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8164993.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9725..12124
                     /locus_tag="IVZ67_000900"
     CDS             9725..12124
                     /locus_tag="IVZ67_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8164994.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12156..14147
                     /locus_tag="IVZ67_000901"
     CDS             12156..14147
                     /locus_tag="IVZ67_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8164995.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14170..14421
                     /locus_tag="IVZ67_000902"
     CDS             14170..14421
                     /locus_tag="IVZ67_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8164996.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14411..15640
                     /locus_tag="IVZ67_000903"
     CDS             14411..15640
                     /locus_tag="IVZ67_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8164997.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15637..17718
                     /gene="topB"
                     /locus_tag="IVZ67_000904"
     CDS             15637..17718
                     /gene="topB"
                     /locus_tag="IVZ67_000904"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8164998.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17867..21787
                     /locus_tag="IVZ67_000905"
     CDS             17867..21787
                     /locus_tag="IVZ67_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8164999.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21788..22732
                     /locus_tag="IVZ67_000906"
     CDS             21788..22732
                     /locus_tag="IVZ67_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165000.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22736..22939
                     /locus_tag="IVZ67_000907"
     CDS             22736..22939
                     /locus_tag="IVZ67_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165001.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23239..23475)
                     /locus_tag="IVZ67_000908"
     CDS             complement(23239..23475)
                     /locus_tag="IVZ67_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165002.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23658..24029)
                     /locus_tag="IVZ67_000909"
     CDS             complement(23658..24029)
                     /locus_tag="IVZ67_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165003.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24026..25354)
                     /locus_tag="IVZ67_000910"
     CDS             complement(24026..25354)
                     /locus_tag="IVZ67_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8165004.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25315..25644)
                     /locus_tag="IVZ67_000911"
     CDS             complement(25315..25644)
                     /locus_tag="IVZ67_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8165005.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25902..26272)
                     /locus_tag="IVZ67_000912"
                     /pseudo
     CDS             complement(25902..26272)
                     /locus_tag="IVZ67_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            26417..26602
                     /locus_tag="IVZ67_000913"
     CDS             26417..26602
                     /locus_tag="IVZ67_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8165006.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27545..28204
                     /gene="vanR"
                     /locus_tag="IVZ67_000914"
     CDS             27545..28204
                     /gene="vanR"
                     /locus_tag="IVZ67_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8165007.1"
                     /translation="MSIRILFVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28207..29550
                     /gene="vanS"
                     /locus_tag="IVZ67_000915"
     CDS             28207..29550
                     /gene="vanS"
                     /locus_tag="IVZ67_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8165008.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         7723 a         7421 c         7747 g         6681 t
ORIGIN      
        1 tgtttctact agtaatgtat ctttttggaa actatgtaac gatgtaaaca agattttttt
       61 cagaagaaaa aacgataaaa gttgtgataa acgatttaaa aaataggaga aaatatatca
      121 agcgcatggt atgaaacgca catttaaaaa gattcgacta actaaaaaaa taagtttaca
      181 aagcattgtt gtgtattaca agtgatctat acactgaaaa atgatagaga aaatctgata
      241 cgtccgcaac aatggacaat tgaaaatgaa agtgaggaaa ttggaagaat ggctgatgaa
      301 aataaagttg ttccgcttga aacactagtg ataaataagg aagagtttga gcaacttttt
      361 cttgattttt ttgttcaata tgacaggatt tatgatttta gttcgtcact agatttattt
      421 gaattagagg cagaaataat agaaaacagt cgagaggaga atggtaaaat catcatttca
      481 gtagaaaaat tcaatgaatt attctcagaa acatttgaaa tagatacaga ttattatcaa
      541 ccacaagaag ttttccaatc atttagaaat cagattattc gctcaggtaa gaccgtaaac
      601 tgaaaaacaa taaaagaaac gttgaaagaa taattattcc ttcaacgttt cttttttaga
      661 atgttttaat gaccaaagca gtttcattgt caggcttagg taattcatga aatcccgtat
      721 ttatagtgct caattgatca actaaccatg ttttactttt tgaattgtag ataaatctaa
      781 aattaatatc gttattatcg tgttgaaggg gcgtctccaa ataatctgct gtatattcat
      841 agtcaacgta acctgctaat acaaaagaga gtatcccttt atttaatgtt agatttaatg
      901 gataatcata cgactttatt tttttcaact tcatattgta catatcttca gccataaaag
      961 ttgagttcgt gatttcatct tgggtctcat taaaaaagtt atccgaataa tcgcttattt
     1021 ttgaaaagtc tttattggca ccagcctcaa aaaattgttc aactttttca gtataaagtt
     1081 gtgctaacgc tcctttttca atattaactt taatattttc atgtctatca atttgtgtgc
     1141 tgttcagtaa gggaagatca gcttttagtt ttatatcgtc ttgatttctt tcaatttcaa
     1201 ttggcaaaat cttttcagta gattcagttt ctgataaagg aacatctagt tttttatcat
     1261 gataataagc ttcaaatcca tctggaaccg gaaaaaaatg tattgggtaa ttcttttctg
     1321 aggttgttgt actagtttcc gtacttgtag aacttgtgtc agaagtggta cacccgctta
     1381 tagttccgat gagtaaagcg caagttagaa tgattgcaaa tttcttcaaa aaaattcctc
     1441 cttaaaattg aatatggact aatactagtc ccattgtagc ataccgaatc tttaagatta
     1501 ataagaaaaa ggactaattt tagtccaaaa ggtgtgctat tcttgaaaga ggaaaaaatt
     1561 attgataaag aaaatgtaat cggtagcaat attagacgag tccgattaga aaaaggaatt
     1621 ggtcaaaccg agctgattag agacctacag cttcgtaaaa ttgctataac tagggaaacg
     1681 ttggttaaga ttgaaggtgg ccgccaacac attaagttgg atcaattgaa agccatcaaa
     1741 gatattctac aggtgtctta tgaagatttg cttcaatgac tacttgtagt catttcgttt
     1801 gaaattatta tgttacaccc ttatgtgtca cgaagaaata ctggtataat aatgaatggg
     1861 atatgtaacg atagggtata ctgttatgtt gcctaataaa ttgtggatca aaatacaaga
     1921 atggagggat atccttgtat aaggcatatg aattggattt tacaaatgct gacttaaatg
     1981 cattctcctc aagctcgcaa tcatatacac atgtagtaaa tcaaatagat cacaaccaaa
     2041 aatctattaa caagagaata gaaaatctat ttgaaagtga aaatgattta gttgattatt
     2101 attctaagga cagcaaaatt ctagatgcag acgaaattat taaagattgg tttccaacta
     2161 taaaagcaga tatttttata tctcactctc atgctgatga aaaattagct atacgttttg
     2221 catcatggct atttgaaaat tttggtctta ctgcatttat tgattcttcg gtttggggat
     2281 acagttctga tttattaaaa aaaatagacc aaaaatattg ctataaggaa caaacaaaaa
     2341 catatgatta tgataaaaga aatgtaacaa ctagtcatgt tcatatgatg ctttctacag
     2401 ctttaaataa tatgattgat agtacagaat gtctgttttt tcttaataca cctaattcaa
     2461 ttagtttgag caatgaaatc actaatgaac agaaatttac gtattcgcca tggctatata
     2521 gcgaactgac gacagcttca atagtggaaa aaaagaatcc acgattggaa agtaatcctc
     2581 aaatgagcac cgaggatgtt agaagcatta taaagcacta ttcttcgcaa gaaaatctgc
     2641 aaataaaata ttctttaaat ttaagtgaat taacttattt acgaaaagct gatttgaaaa
     2701 aatgggcttt agatgctgtg gaatgcacta aaggaagtaa tacattagat attttatata
     2761 aaaacaccat tttcactaat ctctaatcaa tttactgaat cgaaaggaac cgggtagaat
     2821 ggataagaaa gttttaataa agaggttctc tgaagctatt gaaaaaggaa atgcagcaat
     2881 atttgcaggt gcaggattat cgatgtcaca aggatatgta agttggccgg aactacttaa
     2941 tgatcctgca acagaaatcg ggttggattc taaaaaagaa acagacttag ttactctggc
     3001 tcaatattat aaaaatgaaa atggtggatc aagaggaatt ttgaatcaga tacttatgga
     3061 taattttggt gaagaattag aaatatctga aaatcatcga atactagcaa gtttacctat
     3121 tgaaacttat tggacaacta attatgatca tctaatagaa aaatcaatcc gagaagctta
     3181 taaaaaccct caggttaaga aaaattatac tcagttagca acaactaatc caaatgtgga
     3241 tacaattgtt tataaaatgc atggtgatat agatgatgtc agttcgacag taataacaag
     3301 agatgattat gaaaaatatg atgattcgta cgcgttattt aaagaaactt taaaaggaga
     3361 tttgttaaca aaaacttttc tctttttagg atttagtttt acagacccta atttagaaag
     3421 aattttatca gatataagat gggttttgag agaaaaccaa agacctcatt attgtataat
     3481 gagaaaaata ttatagaaaa aattttaggt tatcgctgaa agccccggaa atacgggcat
     3541 ttcgcaggat atgagaactc aatttactac caatttacta ctttcggatt gagccgcata
     3601 tatgctgaac catactgcta atacccgcca ccccaaatac ggcacatcat aaattgaata
     3661 cgacaccgta aacgcggcgt tcctctttcc cgtcatggga gaacaggacg ccgctttttt
     3721 atgcccaaat agagaaagga ggcgacgcca tgctatttac ttccggccct gaccgggcgt
     3781 ttgaacggct gatgcaggaa aagcccggtt tcgatcacta cgacggcggc ggggcggacg
     3841 cgccggagga ttgcggcacc tgccgatttt accgcccgga ttggaaatat caattctgcc
     3901 agtttgcaga gtgtccctat cagccgggca agctgaccga ctatgcagcg gtcaaattca
     3961 cagtgaaagg agcgaacaac gctatggcag ttttccgtgt ggaaaagaac aaaggctata
     4021 cggtgatgtc aaatcaccac ctgcggaata agaacctgac cttaaaggcc aagggcctgc
     4081 tgtcgcagat gttgtccttg ccggaggatt gggactatac cctaaaagga ctgtcctata
     4141 tcaaccggga aagcatcgac gcgatcagga cagcggtatg ggagcttgaa aaagctggat
     4201 atatccggcg tgagcagggc cgtgacgcaa aaggcaaaat ggccgatatg gtctatacca
     4261 tctacgaaca gccggtattg gaaaacccgg tattggaaaa tccaacatcg gataacccgg
     4321 tgttggaaaa tccgacatcg gataatccgg cgtcggaaaa tccaacgcaa ttaaataaag
     4381 aagtacaaag aactgactta tcaaaaaaag aaaaaataat tacagatgga caaagtaccc
     4441 attccattcc tatcccatcc ccgacccctg cccctatgga gggcgaggca gcggctacgc
     4501 cgccggaacg gaaaaggaca ggaaaggatg aggctgtacg gatttatcgg gaaatcattt
     4561 tggagaacat cgagtatgcc tatctcattc aggacaggag cattgaccgt gaacagcttg
     4621 acgaaatcgt agacctgatg ctggaaaccg tctgcaccgc ccgaaagaca atccgtgtcg
     4681 ccggggacga ctaccctgcc gagctggtga aatccaagta catgaagctg accggcgaac
     4741 atatccgctt tgtccttgac tgtctgcggg agaacacaac gaaagtccgc aatatcaaac
     4801 aatacctgcg ggctatgctg ttcaacgccc ccagcaccat ttccaactac tacacggccc
     4861 ttgtcgcgca cgacatggca cagcccgatt ggggaaagcc taaatccggc ctgccggact
     4921 attcctgcgg cgagggcgaa agcctgtaac gagaaaagga ggacgaccct atgaagcagg
     4981 gcgcattgat ttttgacgag cgaacagacc gttacgacat ccgctttgat ctggccgact
     5041 attacggcgg cctgcattgc ggggagtgtt tcgacgtgat gatcggcggc aaatggaggc
     5101 ccacccgcat tgaaatggcc gaaaactggt atcttgtcgg catccgggcc gacgaccttt
     5161 ccggcctgcg ggtgcggatt tgacctatat ctacctgcgg cgtctggtgg ttccgcctta
     5221 gacgccgctt ttgactttct gacaaggagg gatgcgattg caggaagaag ttactcaaaa
     5281 gaccattgcc ctgtccatga aaacgggcaa gctcacggct caagtgctgc aagcggcgct
     5341 gaaaaagtac ctgcaacacc gggccaaggg caaaaacacg ctgcaccacg gccagcagag
     5401 cctaaagcag cttaaaaagc atggggctga tctctccaac attgagatca ccgaggccaa
     5461 cattggagcg ttcaagccat gcgccaagaa atacggcgtg gattttacct tacgaaaaga
     5521 taagaccacc cagccgcccc actacgttgt gattttcaag gccaaggatg cagataacct
     5581 tgatctggcg tttcgggagt ttaccgccaa gacgctttcc aaggagcagc ggccctctat
     5641 ccgcaaggtt ttgtccgctg tgaagcaaaa ggcggcggcc cagaccaagc agcgggccaa
     5701 ggagaaaatc aaggaaaggg ggctggaact gtgaagcctg acgtaaagaa attgctgatc
     5761 ctgaacctcc cgtatctgat ttttgtgtac ctgtttgcga aatgcgggga ggcgtaccgg
     5821 ctggccgctg gtgctgacct gtcggggaag ctcctgcatt ttgcggatgg ttttgcggcg
     5881 gcttttgcca acccgctgcc aagcctgcac ctgtttgacc tgtgtatcgg tatcgccggt
     5941 gccgtgatcg tgcggctggt ggtgtactgc aagagcaaga acgtgaagaa ataccgcaaa
     6001 ggcatggagt atggcagcgc ccgctggggg acggccaaag acatagcgcc ttacatcgac
     6061 cccaagtttg aaaataatat cctgctcacc caaacggaac ggcttaccat gaccggcagg
     6121 cccaaagacc ccaagacagc gcggaataaa aacgtgctgg tgatcggtgg cagcggttca
     6181 gggaaaacga gatttttcgt gaaacccaac ctgatgcaat gctttccgac cacggactat
     6241 ccgacttcgt ttgtggtgac agatccgaaa ggcactttgg ttctggaaac cggcaggatg
     6301 ttccagcagg cgggctaccg cattaaaatc ctgaatacca ttaacttttc caagtccatg
     6361 aagtacaacc cctttgtcta catccattcg gaaaaggacg tgctaaagct ggtaaatacc
     6421 ctgatttcca acaccaaggg cgagggggaa aagtcagcgg aggatttttg ggtgaagtcg
     6481 gaacggttgt tttattcggc cctgatcggc tatatctggt acgaggcccc agcggaggaa
     6541 atgaacttct caacgctgct ggaaatgata aacgccagtg aagcccgcga ggacgacccg
     6601 gagtttcaaa gccccgtcga tctcatgttt gagcgtttgg agcagaaaga ccccgaccat
     6661 tttgcggtgc gccagtacaa gaaattcctg ctgtccgcag gcaagacccg ttcttccatc
     6721 ctgatttcat gcggtgccag acttgcgccc tttgacatcc gggaggtacg cgagctgatg
     6781 gaggacgacg aaatggagct ggacaccatc ggggacgaaa agacggtgct gtatctcatt
     6841 atgagcgata cggacacgac ctttaatttc attctggcta tgctccaaag ccagctaatc
     6901 aacctgcttt gtgaccgggc tgatgatgtg tacggcgggc ggctgcctgt ccatgtacgc
     6961 ctgattttgg atgaatttgc caacattggc caaataccca atttcgataa actcattgcc
     7021 accattcgaa gccgtgaaat atcggcctct attattttgc agagccagtc gcagctaaaa
     7081 gcgatttaca aggacgccgc cgagatcatt tcggataact gcgatagtgt gcttttcctt
     7141 agtgggcgcg gcaaaaatgc caaggagatt tccgatgcgc tggggaaaga aacgattgac
     7201 agtttcaaca ccagcgagaa ccggggaagc caaacgtccc acggattgaa ctatcaaaaa
     7261 ttaggaaagg agttgatgtc ccaagacgaa attgccgtga tggacggcgg caagtgtatc
     7321 ttgcagctac ggggtgtgcg cccgttttta tcggagaaat tcgatattac caagcatccc
     7381 cggtacaaat accttgccga cgccgacaag aagaatacct ttgacgtgga cagatttttg
     7441 actatcacgc gccggaaacg gcagcaagtg gtaacgcagg atgaagtatt cgacctgtat
     7501 gaaatcgacc tgtcggacga ggacgccgcc gctgaataaa cagcggcttt tttcattcca
     7561 acaacaaaat caaaaaatca ttatgagccg gaaaccggca gaaagaggta aatttatggc
     7621 attttttaat tccgcagtta ctactttgca gactatcgtg atcggactgg gcggcgctct
     7681 gtgcgtatgg ggcggcatca atctgttgga gggatacggg caggacaatc cgggggcaaa
     7741 atcgcagggt gtcaagcagc ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct
     7801 ggttcccctg ctgtccgggc tgctgggctg atcctggcct ttgtccctgt ccaaaccctt
     7861 aactaatcgc ctccgcgtcc tcccggccag accgggaggg cgcgggaaag gaggtgctac
     7921 ccgtgattag tgaaatcatt gaagaatgga taaaaggcat cctcatggac ggtatcacgg
     7981 gcaacctgtc cgggctgttc gatacggtaa atgccaaagt cggagaaatc gccgccgacg
     8041 tgggacgaac cccgcaggca tggaacagcg gtattttcaa tatgctgcac agcctgtcgg
     8101 aaaccgtgat cgtccccata gccggggcca tactcgccct tgtcatgtgc tatgagctta
     8161 tccagatgat aacggaaaag aacaatatgc acgactttga cagctccatg ttcttccgct
     8221 ggatattcaa atccgcgttt gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg
     8281 gcgtattcga cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga
     8341 catccattga ttttaacacc ctgataccag accttgaaag ccaattggag gctatgagcg
     8401 tgggcgctct tttggggctg tggttccaaa cccttgttgt gggcctcacc atgaatatcc
     8461 tttccatctg cattttcctt gtgacctatg gccgcatggt ggaaatctat gttgtcacgt
     8521 cgctggggcc tatccccctt gccacaatgg gcaacgcgga atggcgcggc atgggccaag
     8581 ggtatctgaa atccctgctg gcgctgggct tccaagcctt tttaatcatg gttgtggtgg
     8641 gtatctacgc ggtgctgata cagaatatcg gttccgcttc cgacatatcc ggcgcaatct
     8701 ggggctgtat gggctatacg gtgctgctgt gcttctgcct gttcaagacc ggcagtatat
     8761 caaaggccgt attcggggcg cattgatccc aaaatccaag catacacagg aggaacttgt
     8821 ttatttcaac gtacaacatc atttacgctg acccgccgtg gcggtatgac tgcaaaaacg
     8881 tacagggcgc agcggaaaat cactattcca ccatgagcat tgacgagctg tgcgccctgc
     8941 cggtggaacg gctggcgtcc aaagactgcc tgctgttttt gtgggccaca ttccccatgc
     9001 tgccggaagc tctgcggtta atcaaagcgt ggggcttttc attcaagacc gtggcttttg
     9061 tatggctgaa acagaaccgt aaaagcctta catggtttta cgggctgggc cgctggacaa
     9121 ggggaaacgc ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct
     9181 ctgtccatca gtttattatc agcccgattg aacaacacag caaaaagccg gatgtgaccc
     9241 gtgaaaaaat cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt gccagacaga
     9301 aaacccccgg ctgggacgtg tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa
     9361 aggaggtgcc aaatggctta tgtaaccgtc cccaaggact taacccatgt gaaaagcaag
     9421 gttgtgtttg ggcttaccaa acggcaactg atctgttttt ccggggcgct ggtgatcggc
     9481 gtccccttat attttctcac ccgtgactat cttaccaaca gcgcggcggc cctgctcatg
     9541 gtatttgcca tgctgccggg ctttttgctt gccatgtacg agcgtcacgg ccagcccctt
     9601 gaagtggtga ttatgcagat catccagtgc tgcttcatcc agccaaagga gcgtccctat
     9661 cagaccgaca acacctacac cgcccttgtg cggcaatacc agatggaaca ggaggtaaag
     9721 gccattgtcc aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga cccgcgccca
     9781 gcgaaaagaa attgacgcgg taatccgtaa atataagggc gacggcaggc cccacacggc
     9841 gcagcagagc atcccctacg aggtcatgta cccggacggc gtgtgccggg tatcgcccgg
     9901 cgtcttttcc aagtgcattg agtttgcgga catcagctat cagcttgccc agccggacac
     9961 gcagacggct atctttgaaa aactgtgcga cctgtataac tatgtggacg cctccattca
    10021 tatccagttt tctttcctca atcggaaagt tgacccggtg cagtacgcca agagctttga
    10081 gatcgcgccg cagggggacg acttcgacga catccgggcc gagtacaccg gcattctgca
    10141 aaagcagctt gcaaacggca acaacggcat ggtaaagacc aaatacctga cattcaccat
    10201 cgaggccgaa agcgtgaaag cggcgcgagc cagattgaag cgcatcggct ttgacctgtt
    10261 aggctatttc aagagcatgg gggccgtagc ccatgtgatg gacggctggg aacggctgaa
    10321 cctgctgcat ggcgtgtacc acccggacgg cgagatattc aattttgact ggaagtggct
    10381 ggccccgtcc ggcctctcca ccaaggattt tattgccccg tcctccctgt gcttcggcaa
    10441 tgcaaagaca ttcggcatgg gcggcaaata cggcgcggta agtttcctgc aaatcctgtc
    10501 cccggagctg tcggacgata tgctggccga cttcctcaat acggaaagcg gtgttttagt
    10561 caatctccat gtgcaggcca ttgaacagac caaggcgatc aagaccatca agcgcaaaat
    10621 taccgacctt gacgctatga agattgcgga gcagaaaaag gccgtccgca gcggttacga
    10681 tatggacatt cttcccagcg accttgccac ctacggcgag gacgccaaga agctgctgac
    10741 aaagttgcag acccgcaatg aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc
    10801 cgacaccaag caaaagctga ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca
    10861 taactgtccc cttgtccggc tggactacca gcaggaacag ggccttgcaa gcagcctgcc
    10921 gctgggcgtc aatcagatca agatacagcg cagccttacg acttccagcg tggccgtgtt
    10981 cgtccccttt gtgacgcagg agctttttca gggcggcgcg gccatgtatt acggcatcaa
    11041 cgccaagtcc cgcaacatga ttatgctgga caggaagcag gcccgttgcc ccaatgcctt
    11101 gaagctcggc acccccggca gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt
    11161 gttcttaacg acgcctgacg atatttttat ttcagacccg gaggccgaat attaccccct
    11221 tgtcaaacgg ctccacggac aggtaatccg gctgtcgcct accagcaagg actttgtgaa
    11281 cccgctggac atcaacctga attattcgga ggacgataac ccgctggccc tgaaatccga
    11341 ctttgtgctg tcgttctgtg agcttgtcat gggcggcaag aacgggctgg aagccattga
    11401 aaagaccgtg atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg
    11461 cccggagaat atgccgatcc tgtccgacct ccacaaagcg ttgttagatc agcatgtccc
    11521 ggaggctgac cgggtggcgc aggcccttga cctgtatgtg tccggctccc tgaacgtatt
    11581 taaccaccgt accaacgtgg acatcggcaa ccggcttgtg tcctttgaca ttaaggagct
    11641 gggcaagcag ctcaaaaagc tggggatgct cattgtccaa gaccagatat gggggcgcgt
    11701 cacggccaac cgaagccaag gaaaggccac atggtatttt gccgacgagt tccatttgct
    11761 tttgaaagag gaacagacgg cggcctattc tgcggagatt tggaagcggt ttaggaaatg
    11821 gggcggcatt ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct ccccggaaat
    11881 tgaaaatatc cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt ccggggatag
    11941 aaaaatcctt gcggaacgct tgaatttgtc caccgaacag cagaagtaca tcgacaattc
    12001 cgaacccggc gagggccttt tgatctttga aaacgtggtg ctgcccttta ccaaccccat
    12061 cccgcacaac acccagcttt acaagattat gacgacccgc ttaaacgagg tggcgggagt
    12121 atgacccggc gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga
    12181 aagtgacgca ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc accggcgagg
    12241 tggagaacat ttccagccgg gaggccgaac aggatttgtc cgcgccgggg ccgggcggca
    12301 ccgccgataa gctgctggaa cgggccgacg ccatccatga cggccataag gccaagaaag
    12361 ccgcgaagca ggccggggct gtggccgccg agggggacgc tgccctgcac cggccatccg
    12421 cccggctcca actgtcggag gaagaacggg ccgacccgga tttacagccc tatatccgaa
    12481 aatcggacaa acgggccgat aagctggata aggcccgcgc cgccatcccc aagaagcgcg
    12541 tcccggtcaa ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc gtcctgcact
    12601 ttgacaaaca ggacaagggg cctccctccc tgaaacccaa tccggcaggc cgtcctgttt
    12661 ccgaggtttt tctattcggc cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg
    12721 tggagggcgg ccataagggg gaggaacttg ccgagcggca ggccggaaaa gccatccgca
    12781 gcaccatccg gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc
    12841 tattgtctgc caacgcggag tatttctacc agaaatcttt gcgggataac ccggagatcg
    12901 cacaggcggc cagcaacccc atttcccggt tctggcagaa acagcagatc aagcgccagt
    12961 acgccaaggc cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca
    13021 agacagccgg ggcctccacg aaagcggcca aggccgcagc caaagaaagt gaacgggcgg
    13081 cctcctttgt ggcccgccac tggaaagggg cgctggccgt ccttgtgttg gggcttttga
    13141 tgctgcttat catcggcggc ttacagtcct gcaccgccat gttcggcagc gccggaagcg
    13201 gcatcacggc gtcctcttac ttttccgagg atagcgatat gttggccgcc gaggacgcct
    13261 acgcggagct ggaagccgag ctgcaagact atctggacaa ctacgaggcc acccatgact
    13321 atgacgaata ccattttgac cttgacgaga tcagccatga cccctatgtg ctgatttcca
    13381 ttctgtcggc gctccatgac gggatattta ccatcgggga ggtacagggt gatttgtcca
    13441 tgctctttga caagcaatat atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca
    13501 ccgaaaccga tacatggacg gacgcggagg gcaacaccca tacagacacc taccaagtgc
    13561 cgtatgacta ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc catgtgccag
    13621 tctatatcat ggacgaggaa acgctaagtt tctattccct gtatatgtcc acgctaggaa
    13681 accggcctga tctgttttcc ggcagccagt accccaacgc ctccaccctg acgccgccga
    13741 cctactacga cgtacccccg gaggctttgg aggatgccac ctttgccgcc atgctcaaag
    13801 aggcggaaaa atatatcggt tatccctatg tgtggggcgg cagctctccc agcaccagct
    13861 ttgattgcag cggcttcatt tcgtgggtaa tcaatcatag tggatggaac gtgggacggc
    13921 tgggggcgca gggcctatat aacatctgca cccctgtttc cagttcgcag gccaagcccg
    13981 gcgaccttgt atttttcaag ggaacctacg acacccccgg cgtgtcccac gtcggcctgt
    14041 atgtgggcaa caatgtgatg ctgcattgcg ggaacccgat ttcctacacg aaccttactt
    14101 caaactactg gcaatcccat ttttacagtt acgggcgttt accttaacgc cagaaaggag
    14161 ctttcaatta tggcaaccaa acttgaccgc atcgaaaagg acatccagaa aaccaagtct
    14221 aagatcgcgg agtttcagaa acagcttcgg gagctggaaa cgcagaaaac cgaacaggag
    14281 aatttgcaga tcatccagct tgtgcgcggc atgaacatga agccggagga atttgccgcg
    14341 ttcttacgca gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca tgaacaggag
    14401 gacaatgccc atgaagaata agaccagacg gatttcccgt attctccccg cacttctggc
    14461 cgtgatgctc tgcgtcacgg cctttcccgt taccgccctt gcaggcggca atgacccggc
    14521 accggccccg ctgccggagg ctacaaccga acccgctacg ggcggtttgg agcctgaaac
    14581 agacgaaacc ggcaccgcca cggacgggac agaaaccgag gccgaaaaag ataccggccc
    14641 tctgaccggc aaggacatca ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat
    14701 tgccgccacg gacgacggca tccagatcac caccggcacc gacaaaaaag aacctgaaca
    14761 gaccggcacc gtcaccacca acggcggcag gctgaatgtc cgcaccggcg cggggcttga
    14821 caagaccgcg ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg
    14881 cgactggatt aaaattctct tgccggagcg catcggctat gtccattccg actatatgac
    14941 cgtcagcgaa aaggaagtag cggctaccgg cgagggcggt ttttccctct ccattgaccc
    15001 ggaggaaatt gcttctctgc tggaactgtt caatggcggc ggggccagcg ccgccctgtc
    15061 cccggacggg aacctgtcac tgattgacga catcggcagc gccgcgaaaa atggcaagca
    15121 gtttatcacc gtggaaacca aaaacggcaa tgtgttttat ctgattattg accgggacga
    15181 cgagggaaaa gaaactgtcc atttcttaaa tcaggtggat gaagccgatc tgctgacgct
    15241 gatggaggac ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa
    15301 ggccggggct gtgaacacga attgcccggt atgtaaaacc aacctgaccg agtgcagcgg
    15361 cccggagccg caggaacccc agccggagga acccgaagcc ccggaggaag aacccaaggg
    15421 cggcgcgggc ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct
    15481 ttactatttc aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg atttgggcga
    15541 gtatgatttt ggggaggacg aggatttgga ggacgaggaa cccgaaaccg agtttgaccc
    15601 ggaagctgat tttgtgagtg aacaggagga cgaggaatga catataaact tgtaatcgcg
    15661 gaaaaaccca gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac
    15721 ggctatttgg aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt
    15781 gccgacgccg gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt
    15841 ctcccggaaa catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc
    15901 tgttcgctga tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc
    15961 gagggggaat tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag
    16021 cgcctttgga tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa
    16081 ccgggcggcg actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg
    16141 ctgatcggca ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg
    16201 ggccgcgtcc agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat
    16261 ttcaagaaag agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc
    16321 agccgctttt ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag
    16381 gccgtttgcg tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac
    16441 cttaccacct tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt
    16501 gactatgccc aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa
    16561 tatcttaccg acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg
    16621 gtgccgtttg ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa
    16681 aaggtgtccg accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc
    16741 actctggccg agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc
    16801 gtggccgggc cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag
    16861 tttaccgcca agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc
    16921 cgttcttcct taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag
    16981 ctttgcgagg gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg
    17041 ccgcccaagc cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag
    17101 gatatgccgg aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt
    17161 ctggaaaagc tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc
    17221 aaggacggca tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact
    17281 gccgaatggg aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt
    17341 atggacggca tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag
    17401 gaaaagcaga aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt
    17461 gaggctgtct atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc
    17521 atgtggaaga atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc
    17581 gctgcgctgc tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc
    17641 aaaacctacg acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc
    17701 attgaacaca ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc
    17761 cggtaaaatc ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc
    17821 cccaaccccc tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt
    17881 ttctatccaa gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga
    17941 tttgcccacc acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga
    18001 caatccgcag gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat
    18061 tccctatgac atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct
    18121 gggccagctt gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg
    18181 cggctttgag aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca
    18241 tcttcccgac gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat
    18301 ggtggatatg ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat
    18361 tgcccagcag gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga
    18421 atggcagcgc gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc
    18481 ccagccggaa gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc
    18541 cgacaaagcg ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg
    18601 catgaaagtg attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg
    18661 ctatatggct tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct
    18721 gctgatcgcc atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt
    18781 tgaccgccat gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa
    18841 ggttaagcag cagatggaga aaaccgaccc ggccacccag cagcccgtga tcggcgcgga
    18901 cggcaagccc gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt
    18961 ctttgacatc agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac
    19021 cggcgacgtg cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc
    19081 cataagcatt gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg
    19141 cattgccatc aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat
    19201 tgcccatgcc aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg
    19261 gccagaccgc agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta
    19321 ttacgggctg gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag
    19381 ggagttatcc gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat
    19441 aaccgagatc gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca
    19501 ggaggccgtc acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga
    19561 acaggccccg gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag
    19621 cccccagcta aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg
    19681 tctggatgca gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg
    19741 tatctttcag ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg
    19801 ttccctgatc gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa
    19861 aaagcatgta atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga
    19921 tatgctgtta ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga
    19981 acagacggcg cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta
    20041 tttgggggcg cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta
    20101 tcagttgccg gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc
    20161 acaggtacag gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata
    20221 cgcggcggct ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg
    20281 cggggaggaa acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct
    20341 ggccgtggac agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc
    20401 tttggaggat atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag
    20461 cctttccgtt tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt
    20521 agacagcttc ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc
    20581 cgaactgtct acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc
    20641 cgaaccgcag ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg
    20701 ccagacacag gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc
    20761 catcaatgag ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc
    20821 cggcagcgcc acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg
    20881 ccagaaaaag cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta
    20941 cgcccgcaag ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc
    21001 tgtcctgatt gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc
    21061 cagtgaccgc aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag
    21121 caccggcatg ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa
    21181 actggaaaag ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa
    21241 gcatgggacg ctggacggat gcccccatct gatcgaaaga ggcattgaaa acctgaaagc
    21301 ggatatggcg tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctctccaa
    21361 caataacgcc gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga
    21421 ggcggcctat gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa
    21481 ccgcctgcaa atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag
    21541 caacggtttc cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac
    21601 aatccgcgtc gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc
    21661 cttacagctt gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc
    21721 ggccaagacc gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt
    21781 agagtaaatg cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg
    21841 gcagaacacc gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga
    21901 tgccgccagc gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac
    21961 gtactgtttc cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa
    22021 attttacggg gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac
    22081 acagggcggc actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca
    22141 cgtcattgaa accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg
    22201 tgacatcccg gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt
    22261 gtactatgag gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca
    22321 gcacagccgg gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga
    22381 cagccggatt gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc
    22441 taacagcccc aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc
    22501 ttccaccaag gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac
    22561 cgggatgaaa ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt
    22621 gagtattcgc aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc
    22681 cagcccaaaa aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat
    22741 gcactttacc gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag
    22801 aacgataaac aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc
    22861 ccagcagacc ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt
    22921 ccacttcaca gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg
    22981 cgtccggcgt cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga
    23041 taggatgaag tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt
    23101 ttcaatccct taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg
    23161 cgcagcgggt gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg
    23221 gtgcagccct cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag
    23281 tttcagggaa atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc
    23341 ctgtctgatc tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt
    23401 ttccgtggcc cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt
    23461 ttcttgtccg tccatcctga tacccccgtc agactgattt tttctatgat accatattcg
    23521 gaagcatgga accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca
    23581 gcacttcggc ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc
    23641 ttgaaatata agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg
    23701 cgctcggcct ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg
    23761 ttagacttcg gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct
    23821 ttggtgatct cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg
    23881 cacagaagcg aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac
    23941 aggcagttgc cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat
    24001 tgagggtaat tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca
    24061 tacgcaaaat gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg
    24121 cttcgccgta cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt
    24181 tcagggcgta cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag
    24241 cttcatacaa aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat
    24301 tgcggtattc catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca
    24361 ccgccatatc cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg
    24421 cggcggtgat ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag
    24481 tcaggaaatt caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat
    24541 agcctgccga ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct
    24601 tgcgctcttt gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct
    24661 cggtgtaggc ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga
    24721 aagaaataaa ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat
    24781 catcaaaatc ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc
    24841 agctcgtccc tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg
    24901 gcttcaccac ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg
    24961 catagctctg tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa
    25021 taatgtgatt gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc
    25081 cttgcagcac ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt
    25141 cgccgggggc aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct
    25201 gcgccagcag catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca
    25261 ccagcagctt gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg
    25321 ttgacttgat aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc
    25381 ctcgatgtcc tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac
    25441 gttgacgccg atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt
    25501 gacgatatag ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag
    25561 cagcgccatt ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac
    25621 gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt
    25681 tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag
    25741 tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag
    25801 ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta
    25861 cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca
    25921 ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg
    25981 tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc
    26041 cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt
    26101 atgacaaccg gcagactggg aatggcccgt cgttttcgat gttggcaaga tagcgccagt
    26161 caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt
    26221 tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc atatcgcacc
    26281 tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat gcacctttct
    26341 taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat tataatgtac
    26401 cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga aatggggtgt
    26461 gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg ctgtaaagat
    26521 ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa
    26581 aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat ggttttgaag
    26641 agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg gcaggctgct
    26701 ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca catggatgta
    26761 tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag
    26821 ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg attctttatg
    26881 gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta tggggtgatt
    26941 gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt tacatttggc
    27001 gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag gaatatagac
    27061 ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc ggagtgcggc
    27121 aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt
    27181 gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa
    27241 acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga tattctgatc
    27301 cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa catcccaaac
    27361 attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa taatatagat
    27421 acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc cgaaaagaaa
    27481 tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa atggagggat
    27541 ttttatgtcg atacgaattc tatttgtcga ggatgatgat catatctgta atacagtaag
    27601 ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa atgaggcata
    27661 caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc tgcccggtat
    27721 gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca ttctgatgat
    27781 gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg cagacgacta
    27841 tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc tgctgcggcg
    27901 cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc cggaggattt
    27961 cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg aaatcctttt
    28021 gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt cacgtatatg
    28081 gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa atttgcgggc
    28141 caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat tggaggaatc
    28201 ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat cattgtcctg
    28261 ctactgcttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc ttatttcaga
    28321 gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact gattcagaat
    28381 agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa ccaatccttt
    28441 gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa tgccaataca
    28501 tcaaatagtt ttagacccaa ctttctttat gtggtacata gagatgataa tatttcgatt
    28561 gttgctcaaa gcaaggcagg tgtgggattg ctttatcaag ggctgacaat tcggggaatt
    28621 gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa
    28681 atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa cctgaaagat
    28741 gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga tatgcattcc
    28801 atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc aagggaacat
    28861 gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct aaaaacgccc
    28921 atcgcggcta caagcgttct gttggaggga atgcttgaaa atatcggtga ctacaaagat
    28981 cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa aatcatttcc
    29041 gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc tgaaccgttg
    29101 gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc agaggcaaac
    29161 aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga tccgaggctg
    29221 ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc gcagggaggc
    29281 gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg
    29341 ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta tcgcattgat
    29401 caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg
    29461 ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt tttgttctgg
    29521 ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat tt
//
LOCUS       DACCZY010000026        27425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCZY010000026 DACCZY010000000
VERSION     DACCZY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.026"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(86..448)
                     /locus_tag="IVZ67_000916"
     CDS             complement(86..448)
                     /locus_tag="IVZ67_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165009.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(522..902)
                     /locus_tag="IVZ67_000917"
     CDS             complement(522..902)
                     /locus_tag="IVZ67_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8165010.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            984..2159
                     /locus_tag="IVZ67_000918"
     CDS             984..2159
                     /locus_tag="IVZ67_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8165011.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2236..2820)
                     /locus_tag="IVZ67_000919"
     CDS             complement(2236..2820)
                     /locus_tag="IVZ67_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8165012.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3021..4019
                     /locus_tag="IVZ67_000920"
     CDS             3021..4019
                     /locus_tag="IVZ67_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8165013.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4131..4640
                     /locus_tag="IVZ67_000921"
     CDS             4131..4640
                     /locus_tag="IVZ67_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8165014.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4670..5329)
                     /locus_tag="IVZ67_000922"
     CDS             complement(4670..5329)
                     /locus_tag="IVZ67_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8165015.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5331..6095)
                     /locus_tag="IVZ67_000923"
     CDS             complement(5331..6095)
                     /locus_tag="IVZ67_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8165016.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6103..6780)
                     /locus_tag="IVZ67_000924"
     CDS             complement(6103..6780)
                     /locus_tag="IVZ67_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8165017.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6817..8202)
                     /locus_tag="IVZ67_000925"
     CDS             complement(6817..8202)
                     /locus_tag="IVZ67_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8165018.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8412..8496)
                     /gene="ffs"
                     /locus_tag="IVZ67_000926"
     ncRNA           complement(8412..8496)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVZ67_000926"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8519..9034)
                     /gene="tadA"
                     /locus_tag="IVZ67_000927"
     CDS             complement(8519..9034)
                     /gene="tadA"
                     /locus_tag="IVZ67_000927"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8165019.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9140..9997
                     /locus_tag="IVZ67_000928"
     CDS             9140..9997
                     /locus_tag="IVZ67_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8165020.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10078..10413)
                     /locus_tag="IVZ67_000929"
     CDS             complement(10078..10413)
                     /locus_tag="IVZ67_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165021.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10480..11850)
                     /locus_tag="IVZ67_000930"
     CDS             complement(10480..11850)
                     /locus_tag="IVZ67_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8165022.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11928..12464)
                     /locus_tag="IVZ67_000931"
     CDS             complement(11928..12464)
                     /locus_tag="IVZ67_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8165023.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12773..14254
                     /locus_tag="IVZ67_000932"
     CDS             12773..14254
                     /locus_tag="IVZ67_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8165024.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14357..15064)
                     /locus_tag="IVZ67_000933"
     CDS             complement(14357..15064)
                     /locus_tag="IVZ67_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8165025.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15143..15481)
                     /locus_tag="IVZ67_000934"
     CDS             complement(15143..15481)
                     /locus_tag="IVZ67_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8165026.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15592..16554)
                     /locus_tag="IVZ67_000935"
     CDS             complement(15592..16554)
                     /locus_tag="IVZ67_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8165027.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGEGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16569..17900)
                     /locus_tag="IVZ67_000936"
     CDS             complement(16569..17900)
                     /locus_tag="IVZ67_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8165028.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(17908..18588)
                     /locus_tag="IVZ67_000937"
     CDS             complement(17908..18588)
                     /locus_tag="IVZ67_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8165029.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18807..19286)
                     /locus_tag="IVZ67_000938"
     CDS             complement(18807..19286)
                     /locus_tag="IVZ67_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8165030.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19413..21092)
                     /locus_tag="IVZ67_000939"
     CDS             complement(19413..21092)
                     /locus_tag="IVZ67_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8165031.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21640..23142
                     /locus_tag="IVZ67_000940"
     CDS             21640..23142
                     /locus_tag="IVZ67_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165032.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23155..23817
                     /locus_tag="IVZ67_000941"
     CDS             23155..23817
                     /locus_tag="IVZ67_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165033.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(23918..24121)
                     /locus_tag="IVZ67_000942"
     CDS             complement(23918..24121)
                     /locus_tag="IVZ67_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8165034.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24232..25416)
                     /gene="iadA"
                     /locus_tag="IVZ67_000943"
     CDS             complement(24232..25416)
                     /gene="iadA"
                     /locus_tag="IVZ67_000943"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8165035.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25573..27312)
                     /gene="yfcC"
                     /locus_tag="IVZ67_000944"
     CDS             complement(25573..27312)
                     /gene="yfcC"
                     /locus_tag="IVZ67_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8165036.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8159 a         5737 c         4653 g         8876 t
ORIGIN      
        1 aacatttcat tgtatttttt ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa
       61 cttctttgtt cgcttaatct gtattttaga agaaaggcac tccctctact tcgtaagttc
      121 caagaacaga gcgcatttga cctggttctt cccaaatata tccttcgcct aattcttctc
      181 tttgattctt ttgttcagtt tcaaagacaa acaacggaac attttcctct ttaatatgtg
      241 tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat atgaactatt tcttttaaga
      301 aattaagtat attcgctaaa ccagtctttc cttcagaaag ttccgctaac gcaaattcca
      361 acttgtcatc aattgaatgg accaaaaatt ttttagtacc atcctccaaa tgcaacataa
      421 ttgtaccagc aactcttttg cttgccaaaa tttccacctc gatcaacttg ttctatggct
      481 tacataaaaa ttctagcaca ttttcagaaa aaatacactt tttatttctt attttctttc
      541 cgttctagtt cagataatgc ttcttctatc caaataggta actgctccgc aatagtggta
      601 aatccgatct tacgattttt attcgtgaaa tacctttttc tgcgatatgg aacagctgga
      661 tttgtttttt ctaacgttct taaagataaa ctgatttttt tggaatattc atcaatgtcg
      721 atgatctgaa ctgttactgg ctgtccgact tttaacaatg aatggatatt tttcgtataa
      781 ccggattgta cttctgaaac atgaataagt ccttgcgtct cgttatcaag agaaacgaat
      841 gcgccataag gctgtatccc tgtcactttt ccttcaatta tttgtccaat tttataagtc
      901 atctacttcc ctctcttcgc atttatcatt atactgtaaa tagctcaaaa aaagaagcga
      961 cgaaaatgac tggaggtctt aagatgatcg aatttgataa acagatttca agaaaagata
     1021 cagaaagtgt aaaatgggac gcaattgctg agacatatca aatggatgac ttacttcctt
     1081 tgtgggtagc agatatggac ttcttgtctc ccggtggggt agctaaagcg tttagtgaat
     1141 acatcaaaca tggaatcttt ggttatgcca ctctgtctga taaactatat caggctatca
     1201 ttcattggga acgccaacac catgctgtgg agttgacgaa agaaaatatc gtttttacaa
     1261 gtggtgtgtt aaccagttta gctgcagcag tacaaacttt tacaaaacca ggcgattcta
     1321 ttttgattca tgacccagtt tatcctccat tttcctctat tatagaaaca aatcaacgtc
     1381 acatcgttcg aagctctttg ctagaaaaaa ataatcattt catcatggac ttgtctgaca
     1441 tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat cctcacaatc
     1501 ctggagggcg tgtttggagc aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac
     1561 atcaagtgat tctgtttagt gacgagatcc atcaagattt aactttattc gatcatacat
     1621 ttacttccat gctaacgatt gatcctaatt tagatgatct actagttgct tttacttccg
     1681 caaccaagac tttcaatcta gctgcaatca aaaattcaat ggtatttatc aaaaatccag
     1741 agatgaaagc tgattttgaa aaacatttag tgatgaacca acagcacgaa atcaacactt
     1801 ttggactgat tggaacccaa gcggcttatg agacgggtgg agaatggtta agccaactga
     1861 ttccttatct agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag tatcttccga
     1921 aagtgagaat aatgaaccct gaaggaactt acttaatgtg gttagatttt accgcctata
     1981 tttcagacga ccaagaattg gagaacactc ttgttcggaa aggaaaagtc gtattaaatc
     2041 ctggcatcac gtttggtcct tccggtcatg ggcatatgcg cttgaatatt gcttgtccag
     2101 aagaaacttt actggaagga cttaatcgaa tcaagtatgc tttagagaca atcgattaaa
     2161 tcatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt cataattact
     2221 ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat catgtacagg
     2281 tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca tcccttctag
     2341 tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac ctttatacgc
     2401 ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat tagctggcac
     2461 atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg aatttgccat
     2521 agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa aactttcgcc
     2581 ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga tcatgaaatc
     2641 aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa gttcaatgaa
     2701 attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt caccacggtt
     2761 tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg ggaatgccat
     2821 attgtttcct cctaatattt gcatacttca taataataat aacataatac ctaacctctc
     2881 gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac
     2941 cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt ctgatatagt
     3001 gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg
     3061 tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca
     3121 gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata
     3181 ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa
     3241 ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg
     3301 aagttatcga gattactaca aataaaggca tccattattc caaagcagtc attttagctt
     3361 taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa
     3421 accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta
     3481 ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg
     3541 aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct
     3601 tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata
     3661 atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga
     3721 tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt
     3781 ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc
     3841 caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta
     3901 ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg
     3961 aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat
     4021 tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat
     4081 gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca
     4141 aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag
     4201 aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg
     4261 cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta
     4321 tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac
     4381 atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg
     4441 tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg
     4501 gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata
     4561 tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa
     4621 gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt
     4681 ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata
     4741 attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat
     4801 tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat
     4861 gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca
     4921 gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat
     4981 atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa
     5041 acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca
     5101 agctgaggat ggttttgaag ggattattga gataattcat taggtgatca aaatttttca
     5161 gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata
     5221 cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata
     5281 ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata ttagaagctc
     5341 cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct
     5401 tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg
     5461 atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact
     5521 tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg
     5581 gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta
     5641 ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt
     5701 gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga
     5761 gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg
     5821 ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta
     5881 gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct
     5941 actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt
     6001 tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt
     6061 ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt ccctttttct
     6121 gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt
     6181 ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga
     6241 tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg
     6301 gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc
     6361 aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc
     6421 ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc
     6481 tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt
     6541 aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact
     6601 gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat
     6661 ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt
     6721 tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat
     6781 gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt
     6841 caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc
     6901 aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata
     6961 atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata
     7021 tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat
     7081 aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc
     7141 atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg
     7201 atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc
     7261 tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc
     7321 attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa
     7381 ttcatgccca cgttctagac aactatttat ttcttgttca tcttctgtaa aagctgtcat
     7441 tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt
     7501 taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt
     7561 gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc
     7621 tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa
     7681 aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg
     7741 tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc
     7801 gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg
     7861 gagcgtattt ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa
     7921 atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc
     7981 ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg
     8041 atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt
     8101 ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg
     8161 agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct
     8221 ttatctttat cataccatag gcagcttttt cttccattga aacaagctct atttcagagt
     8281 ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa
     8341 aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg
     8401 ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga
     8461 cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt
     8521 atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga
     8581 aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt
     8641 ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg
     8701 gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca
     8761 taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt
     8821 tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt
     8881 ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg
     8941 gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt
     9001 ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc
     9061 ttgttttact ttagcatatg ataaaaaggc tgtaactaac tttttttcta tggtaaaata
     9121 aatacaagag gtgaacaaga tgaaaatgga taaattactt gctctttacc cacaagcaca
     9181 gaaaaaaaac tctccagcat cagaagcgga tatcctttct tttgcaattg atgatgcttt
     9241 tatctggatc aaacaagatt cattatctca acaagaaacc agccttttga aagcgttatt
     9301 ccctgtaatc aacgatcaaa aaaaacaccc atggtatcac tatcttttta aagatgcttc
     9361 ttgtgttcaa gaaaaaagct ttcgtttgat ccagcttcac gtcgaatcaa aaggagaatt
     9421 cttaaaaaaa gaatggcaag atactatttt ggaattattt cgctattctg ctgatttctt
     9481 tttttataca gacacagatg cgctcttagt cgaacaaata aaccgtgatt atcttgatgc
     9541 tactgaaatc aacggtattt ttctatcttt agatgctgat tttgatacga caacgtctgt
     9601 attcgttggt tctttccatt cacctggcac cgacctcgtc cctttgtttg ctgaagaaag
     9661 aaaaattttt ttaacagagc aaaacaatct ttatactcac agccgtactt tttctatgca
     9721 agatgtggct ttacattact atacaaaaga tactgtaaaa gatagtaagt tgatcaaaag
     9781 ttacatagaa ctgatcaaac aaaatgaaat ggaagtgatc attcaagaat tatggaatga
     9841 attaggcaat gtcagctctg ctgcaaaaac actgtttctt catcgtaata ctctaaaata
     9901 tcgaatcgaa aaatttcaag aacagtctgg tttcaatcta aaaaaagcca acgatttact
     9961 gttttgctat cttttattgc ttcatgaaca taattaaaga tagattaaag gggttgtgac
    10021 aataattttg tcacaacccc ttatttcgaa tgaatagtgt tcaaaagcta gtttctctta
    10081 catcattttt tcaacaaaat gcattagccg gtcttttaca tcttcggttg aatctttgat
    10141 tgaatcacca taaacagaga ttttcttttt tcctgatttg atttttgcta acattcccat
    10201 caaagaatcc aagtcactat ctgaaagacc atctacgatt tctagtaatt tttcatttcc
    10261 atcaaatttg tcatagacga atttttttac tttcgtccga gtagtcatat gatggatctt
    10321 ttccattact tttcccgata caacgactgc tgtcgctaca ctggcagcgg ctgctacgct
    10381 taatccgatt gttacttttg ttgatgtttt cataagtatc agctcctttt tatctatctc
    10441 catcatagca tagacaaaaa aataaacgaa ctaatccact cagtgaaatc tcaatccttt
    10501 agggaaaaag ttctttactg ttccttttac ttgtttccca aatccgacag gtatcttatc
    10561 taatactagc aaaacccatc cttgattccc atcacgttga aatgtttctc ctgctgtgta
    10621 agattgccat tccttttgag tgatcggcaa gcaaggtata ttctctatct tttttgtagc
    10681 aagcgctaat gcatagctag gttcaaatcg attctttttg aaatctccta aatgcaaacc
    10741 tgtacgtacg acttttaaac catcgagaga aggggctaac tcaggaactt cccaaagatg
    10801 gtcgttgaac actaaaagtc ttccagtagc ttcataatga aaatcatttg aaaactccgt
    10861 ccagagtttt tcctgctctt ttgtcatttg cactttactt ttttttcgtg tcttcttttc
    10921 tttatgcatc tgattttgtc catgaaaagt cagctttgca acaaaatgtc cttcaccctg
    10981 atctttatga ggccatatac gtattgtttt ttccaaacca gctacgcttc cccattcaga
    11041 acgtcctgaa ctaacggatt gagtcaaagg aatctcctca atcgttactg gataattttc
    11101 tacaagccag ctaatgatct cttcattttc ttctggtgca aacgtacaag tcgaataaat
    11161 cagttgtcct ttatttttta gcatttttat tgctgaagac aaaatctctt gctgacgttt
    11221 ttgacagtat agcggagatt cttctgtcca ctcttttatt gcatttggat cttttcggaa
    11281 cattccttct cctgaacaag gagcatcaac cacgatccga tcaaaaaagc ccgaaaaatg
    11341 agggacaagt tctgcgggtg catgatttgt cactattgcg ttcgaaactc cccatcgttc
    11401 tatattttct gataaaatct tcgcacgttt cggaaagatc tcattcgtca ctaataaccc
    11461 tttccccttc atttgagcag ctaattgggt tgatttacca ccgggagccg cacacaaatc
    11521 caaaactttt tcaccgggtt ttgctgcagc agctgttcca acgatcattg cacttggttc
    11581 ttggctatat tcatacccag cttgatggag gaaagacttt ccattgacag tgccaagaaa
    11641 tccctcattg ctgtagggag cgggttgtaa ttcttcgcta tggtaagttt ggaccatgtc
    11701 caatcctgcc ggttttagtg gattccaccg aaatcctttt ttcacgcttc cttgttcaag
    11761 cgcagaaaaa aaatcggatg cttcttcgcc taaaagaaga cgatattttt tgataaattg
    11821 ctgtggtaac gttgtcgctt cctccttcat tccaatgttt cgttagacat tttacaatct
    11881 tttttacgaa aaataaaggt ggctgatcta ctttttcatg ttgctgccta aggtgactgt
    11941 tcctttaaga tattttccaa gtattcatcc gtcacctcgt ttttatccca aacagcgttc
    12001 atcatttcaa gaaaatcttg agaagaatca tctgagcgca aacttcccaa tgcattaagc
    12061 cataccaatt caagcgactt ttcttctttg atcttcgtgt gacctttgac atatgagaaa
    12121 aatggccgct taaatagcga atccatgtct aaatcagatt ttgcaatgat ttcacgaata
    12181 ctttcatgga tagaaaaaaa atctttcaat aagtagacaa tgcttaaaac atcatcggaa
    12241 ctgcattcag ctaagtagtt ttcaaaataa acagtgattt cttctaatat cttctctttt
    12301 aacccgctga ttgatttgaa atgtgtataa atcgggtatg tagaacaacc gcagaactct
    12361 gctaactctc ttgcagtaat tgacttcatt ccatattctt ccacgtatac catacttttg
    12421 aacagcaaat attctttact ataatttgtt actctcccca tcattcttac ctccgtctct
    12481 tttcatctac ccataaataa ataacataaa taattctaaa aatattattg ataattatca
    12541 aaacatattt agttatatcc attaaaaatc aatttttccg ccttaaaaac aagaatatct
    12601 tgacaaaata ccctaggaaa taaacaatag aataaacaag gtaacaaaaa aattaaataa
    12661 cgttatataa aaaagaaggc gacactctgt gagaataaca cagactgaaa tgactttcca
    12721 ctcacagttt ttgtctaact atatacaaaa acagaataaa aaggaggtat ttatgctaat
    12781 tgaagcaaga aaaaaagagt tacttgttct acaaatggtc attcaaaaaa ataattgttc
    12841 tttacaagaa ttatccaatg acttacatat tccaaaacgg acaatcaaag aactgatccg
    12901 aaaaataaat gtatcactca agcaattttt attgattgat gaatttattt attctaatca
    12961 taaaggagag atccaaacca aagaaaccga tcaaactaaa aagttagctg ttttttctaa
    13021 gttaaaatta tgctacctaa aagaatccaa tcgttttaac tatttgatgt tactaatcaa
    13081 ttatccacaa actgctgtac caaaaaaata tttacttgaa caactttata tatcgccctc
    13141 ctatctcgaa aaactgacaa aacagctcaa tcattcctta ataaaattcc atttacggat
    13201 cctatctgta aaaaattgct gtactcttga aggaaatgag ttatctatcc gtctctatct
    13261 ttactttttt ctttcagata catttggagg taacgattgg ccattttcct cacttgaatt
    13321 aaatgcacta aaaatgaaca gtacagatga gcaaatagaa aaaatttcaa aacaagaaaa
    13381 aaatatctac ttgctgttaa ctttattcat attacgaaac aaacaacagg catattttcc
    13441 aaaaacttct tctgaaatca ctgaattgat ggacttattg cagcatacac atgattattc
    13501 agaatacttt acttcttttt cagtgagtcg cccttgtgaa gagacagaaa cagagagagc
    13561 ctactttaat tattttttct attgtttttt tccaaatcaa atctcttttc gacaaaaaca
    13621 ggagattggt tttttatttt ccacaagcaa aaaccttatt tgtcgacaag tcggtcaatt
    13681 gatgacttac ttaactcaac agtttcctct caaccattta gaaaataaaa taccaattta
    13741 tttttatttt cttgtgttgg cagttgtttt tttcgaactg ttgggtgaat ccagcagtag
    13801 tttttctaca ctttgctttc cagaagccac gtccaatcat tctagttctt ttatacagga
    13861 aaagaaatta agagaaaaaa tcgatacaat tttttcaaaa aatggggaaa gaaaccaaat
    13921 cagtcaatac cttagtcaat tgatccaaca cttactacaa acagaaacga aagagcagtt
    13981 actcatccat ttgcagataa ccaaacagtt tacaggaaat tatcatcttc aaagccattt
    14041 gcagcagtta tttcgcaaag atgctgttaa aattaccgca tattcagaac atgctgattt
    14101 gatcattaca gatcagcatg agaaaatttc agatgaaacg gagactttct atctggatac
    14161 aacgcacaat acagtgttat ttggcaagtt aataaaagcg gttcatgaac tgtatatgaa
    14221 aaaaggttat gagaacaaaa caaagaggat gtgacaaaat tcttgtcaca tcctcttatt
    14281 ccaaataaat ggtattcaat cagctaactc tcggtattag ctcagatcaa agcgctgatt
    14341 tcacaacttc tttattttaa tctcttcggt tgccgccgaa caataagatc aagcgcaaca
    14401 gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag tgctgctgca aataatacgt
    14461 gtcttgccat cggtacttct tcttccgtca gtagtccgcc ttcactcaaa atttggattg
    14521 ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa ttggaaaagt aatgctaaag
    14581 aaaaagcgaa aatcccgata ttgattaacg tagagttcca gctaagcaat acacctacca
    14641 taatcaaggg gaacgaaatc atcgaaccaa agttagctgc tggaacaagt gctgcacgaa
    14701 tcttcaatgg gacataaccc gaagcatctt gtaccgcatg tccgcattca tgagctgcta
    14761 cacctatcgc agcaactgaa gtagactgtg cagttgcttg agacaaactc aacatcttat
    14821 tacctgaatt gtagttatct gtgagatctc cggcaatttg ttgaacaccg acattattta
    14881 tctgttgact ttgtaatata tattgtgctg cttgtgtacc agtcacattg tttttgcttc
    14941 tcacttgatc gtattttcgg aaagtactat tgacgtacgc tgacgcaatt ccggaaataa
    15001 ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa accatacatt ccataaccat
    15061 acattttcgt ttcactcctt ttcgtttact ttattttatc attagtagta ggaaaagaat
    15121 gtgaaaagac cttgaatatt ctttaattct ttttcacaaa ttccgatttc agtttcatcg
    15181 gtccaaatcc ttctaccttg caatcgatat tatgatcgcc ttctactaaa cggatgttct
    15241 tgacttttgt tccttgttta atggcacctg acgcaccttt tactttcaag tctttaatca
    15301 ctgtcacgct atcgccctct aacagacggt ttccatttgc atctaatacc actaatccgt
    15361 cttctatgac agtttcttgt tcttctttgt tccattcgtt cccgcattct gggcaaatca
    15421 acaagcctcg atcttcatat gtgtactctg agccacattc tgggcaattt ggtaatgtca
    15481 tcttctttct cctttaaaat ctttagtgag cactattgta acggaaaaag aagatcttga
    15541 aaagatacat ttgtgatttt tactacaaaa ggcacgtttt cgaacgaagt atcattctgt
    15601 ttttctgctc ttatacgatt ggcatatatc ttggatctct ttatagattt cctctacttc
    15661 tgctaaatct tttgcattcg caccacttgc taatggatgg ccttctccat gatggcgttt
    15721 agccaattca ttgatgatcg gccccttaga acgtaaacgt acgcggtaat accctgaagg
    15781 ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc actccaggaa gtgaaacgat
    15841 cgaagctgtc tctgaatcat cgattccata tttatcaaga atttcttgcg gaaggattat
    15901 tcttcctgca ccattttcat ccacctggat attttgataa agataaccgg aaagtttagc
    15961 gactttcatc ggcatttgtt caatctctct gttcaaagca cttgcatcaa atggataatt
    16021 tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg gagggataca aaaagcgccc
    16081 tgtatctccg acgattcctg cataaagtaa ccgtgcagcc ttaacgttca tctttaaaga
    16141 ttcaggaaac atcaaagcaa attctgcaat catctcacta cagctacttg cttttgtatt
    16201 tacccacaca agatctccat aagggtcatc attgggatga tggtcgattt tgatcagttt
    16261 ttctgccaac tgatatcttt gatcactgat tctgggtgaa ttagctgtat cagtcacgat
    16321 gactagcgct ccttggtaga agacatcttc aacttcttgc atttctgcta aaaagcgaag
    16381 cccttcaatc gtttcaccta cttgacgtat ttctttttct gggaaacttt cccgtaatag
    16441 ttcggctaat cctacttgtg atcctaatgc atcaggatca gggcgcaaat gcctgtggat
    16501 aatgatttta tcgtattttt ttattgtatc tactatttct tgctgtactg tcatttcact
    16561 cctgctttct aagaacgttc cattacttga caaacaacga ttgctttgga aacgatggtg
    16621 ttttcaataa acacttcgat atccagttta gcggaacgtc tgccgatttc caatacacgt
    16681 ggacgaatgt ctaattcgct ttccaattgg atcagccgca aataatgtag gttcacttgt
    16741 tcaatcaaga tatttcgttt ttggttcatc accattgttt tttgtgcgac atcagaaatg
    16801 atttcgctaa gtacgccaaa agaaatggtt ccaacgccat tgaccatttg tggtgctact
    16861 acgaatttga acttagggtt ttccaaacga tttccttcac ggtcagtatc aatcgtatgg
    16921 atatttccag atatctgatc cgaaatcgta tctgcaatct gcggttgtcg ctggacaagc
    16981 tgcatcgctt tcattacatc ttgccgggta atcaagcctt ccaatgtcaa gtcatcggaa
    17041 acgaccggca tgacttctag cccatcccag atcatttgat ggctaacaga ggcaatactc
    17101 attgttttct ttacactgat tggttctttc gtcatcaccc gatcgatgat ctggtttggc
    17161 gatttcccta aaacatcttt tgccgtaaca atccccatca aacgatgatg gcggttgacg
    17221 actggaaaac gcgaatgctg cgtttcttct gacaaacgct gataatcaga aatcgtatct
    17281 gtctgctgca aataatgagt cttttcagct ggcatataga tatcactgac aagcatgata
    17341 tcttttttga tcaattggtc gcttaacgct cggttgatca tggttgcgac tgtaaatgta
    17401 tcataagagg tgcgtaagat cggcatttca agatcatcag ctagctttgc aatctcagca
    17461 gtcgtattga atcctcctgt gatcaatact gcggcgccat cttccaatgc taatttttgg
    17521 acttccgccc ggttgccgac gatcattagt gatcctggcg taatatagcg ttccatggca
    17581 tgtttagtca tcgcgccgat gacgaattta ttcaagacct tgtctaatcc tgccgccccg
    17641 cctaaaacgt ccccttcaat gatcctgacg acctcgccaa atgtcaaacg ttcaatatgt
    17701 ttttttaatt tgcgttcgat acggatggtc cctactcgtt gaattgttga aactagtccg
    17761 acattttctg catctttgat tgcacggtac gctgttcctt cactgacatt caattctttt
    17821 gcgatgcttc ttacagaaat tcgattgcca acaggtaaac tttcaatata ttctaaaatc
    17881 aaatcatgtt ttgtcgccat tttctgccta cacttccagc gtttccgaga cgctcattat
    17941 ttttccttca ctatcttcca gtaactctac aaaagattcg ggatcttgtt caatgacagg
    18001 gaaagtattg taatggattg gaactacttt cttggcacga attgtttgcg cagcctttgc
    18061 tgcatcttct ggccccatcg tatagttatc gccaatcggc aagaaagcga catcaatatc
    18121 gaattcttct gcaagcaatc ctagatcact gaaataagca gtatcccctg catgataaat
    18181 cgtttttcct tctgcttgaa taataattcc gctaggctcg cccatatact gaacgatacc
    18241 gtctacttca tagcttgaac tatgctgtgc atgtacgaat tttaccacgc cgaatggaaa
    18301 cgcatgtttt ccaccgatat tcataccgaa tgaatctact cctctgctgt tcgcataatc
    18361 agcaatctca acaatactga taattgtggc ttgattttct tttgcaatag gaatcatatc
    18421 tcctatgtgg tcactatgac catgagtaat cagcaaccaa tctgtcttta cttcttctgc
    18481 agttaagtct gtcaatggat ttcctgtaat aaacgggtca atcatcacgc gttggccatt
    18541 ttccatttca attgttaaac aggaatgtcc atgatacgta attttcatat tttacacctc
    18601 gcgttttctc tgtatctttt ttattaaaaa cgatttcatc acttgcacat caatgaaaaa
    18661 atatagctgc taaatatatt ttcattggat tgatagtaca gtatgtacaa agcaattgta
    18721 acagataata caggatgcac caagtaccaa gcagatataa tctcaagttt gaggtctctg
    18781 ctctgcttag cttctcttct cgtcccttaa ctaattaaat cagcgtaaat aggatggacg
    18841 atcttttcta aatcggctac ttttatccga aggcttctcc cgatttcacc gcttgacaca
    18901 attacttctt ctttttcttt taaagaactg tctgctaaaa aacaataatc aggatgtgcg
    18961 agttttattc caataggggt gtttgctccg tggggatatc ctgtatgcat taatacaaaa
    19021 tccattccag gaaaaccaac tttacgatct ttcgtcactt gtctcgtttt tttgtaatct
    19081 aagtgatcgt tcaaaggaat caatgcgatg atcaccccag ttttgtttcc tttcactacg
    19141 agagttttac atatcaaagc tggatcaatc cctctttttt gtagttcttc tgtgaaagat
    19201 tcttctacca catgactaaa atcaagtggt tgatagctga tcttttgttc ttctaaataa
    19261 cgttcgactt ttgtttgact catcattgat tcactccctt tattctatcg tttattataa
    19321 agcgagggat gctgtattca aaaattattt gtttttcgca aaaaaagagc aagacgagca
    19381 ctctatagag aggttcatct tactcttttt gtttattttt tatttcgacg ttgagtagct
    19441 tccataatga ctggcaagat cactgggcgg cgttttgttt gttcaaataa gtaacggcct
    19501 aattgatctc ggatgtcttg ctttaatttg ctccactcaa aatcattgct ttctaaatgt
    19561 ttctcaacga ttgtttcaat catattgctg ctttctttca tcagatcacg gctagctttt
    19621 acgtaaacga atccccgtga agtaatttga ggttttgaaa tgattttctt ttctcgccga
    19681 ctgatagtga caaccgctac gaaaatcccg tcttctgaca agatttttcg atcacgtaaa
    19741 acaatgtttc cgatatcacc tatgcctaat ccatcaatca ttacattttc tgcacttgta
    19801 gttcctgcaa cactcatacg cttgttctta tattccagca cgtctccgcg tccagtaata
    19861 tagatatttt tgtatggcat ccctactgca tgagccaaat ctgcatgagc agctaattca
    19921 cgatactctc cttgaactgg cacaaaatat gttggtttca tcaaattgat cattaattgc
    19981 aagtcattag gattggcatg gccagaaacg cgcatattct cagagatcaa cttcactgtt
    20041 ccaccagctc gataaatgat atcttccgtt ttcgccacga tggtttccat cgcgatgctt
    20101 ggcgtagtag tgatataaac caaatctcct tcttcaatct tgacattacg gtgtgtaccg
    20161 tttgccattt tttgcagaga cttgatgggt tcacccattc ggccagtttc taaaatgatc
    20221 agttcttctg gatcatagtt tttcatgtct ttcaacgtaa caagcagatc ttcacttggt
    20281 aattgaagtt tttctaaacg catcgctgtg cgaataattc gttcgaaatc ttgaccagtc
    20341 aagacaactt ttcgttgtgc tttttctgct gcatccaata cttgttgcac tcgttgcaaa
    20401 ttacttgcta cacaagcgac gatgatacgc ccttcccaat atttgatcgt atcaaatact
    20461 tcatctgcta tttgggcttc agaagctaca ggctgaggat tttctgcatt tgccgattca
    20521 cttagtaagg ctaagactcc ttctttgccg atttccgcca atcgtgcata atctgtttga
    20581 tacattggga tagccgtttg gtcaaattta aaatctcccg tataaacgat actaccttct
    20641 tttgttttca gattgatccc cactgaatct ggtatcgtat gagtggtacg gaaaaagctc
    20701 actactgttt caccaaagtc tatttctgta tgctcatcta ccacatggaa atccttaaat
    20761 cctttggctg cttcattacg actgacattc aattttgcta actcgatcgt caactctgtc
    20821 ccgaatactg gtacagaaat ttttgacaac aagtaaggaa gtgccccgat cgcatctgca
    20881 tgtccatgtg ttaaaaagac accagctaca cgatcaatgt tttcttctaa atacgtaaaa
    20941 tcagggatta cgacgtcgat ccccaacaac tcattttcag gatattttag cccacaatct
    21001 aaaataaaaa tatcctcgcc cacttctgcg acatacatgt tttttccatt ttcacgaacg
    21061 ccgcctaatg ggataatttt aatggtactc acaaacttca cctcttctta tgacatccaa
    21121 gtttaaagat gtcgattttc ttctttatct aaatatatga gccattccaa tgaagcatat
    21181 gcaaaatcac tatttctcaa cttatggaga atcagcagga aaattaccag cacatccgcg
    21241 ttctgacagt tggatgggca ttttattttg gatatacttc attaacaaaa tggatctttt
    21301 gcgaaacgct atagccagaa tacttatata agtattcagc tgttccccat tattatacaa
    21361 caaataaact aaatcgctag tatcagcaat tgttagaaca actatgcacg taaaaatccc
    21421 tctgtagcat ctaggatttc ttttgctatt cctctttcat ttcttcttcg tcggtttgaa
    21481 acatactttt ttcattttac cacatttttt gcaataacta tagtgacttt ttatatatac
    21541 agaacctcat gttgatggtg tgcaaacagc ataagattta agctagcttt aagataggtt
    21601 gtctattatc aaatgcatct actaggaggg atgcaaaata tgaattttat gaaacgtgca
    21661 tggaaaagta caaaagtaaa atggggacgt tcattgttat tgtttgccgt gttcacagcc
    21721 attcttattt ttgtgctagc tggcctcacg attcgaagcg ctgcagaaga agcagcgaat
    21781 caagcacaaa aagaagttgg agcaacagtg actttaagtg ccaatcgaga agcttcattt
    21841 aaaaaacagg aacaaacttc ttcaagcggt tctaccactg aagaacgtcc agatccaggc
    21901 agtttttctc ttacaccagt ttccttaacg gacgcggaaa aaatagctga attagataat
    21961 gtaaagagtt attcctttga aagttccgcc tcagctatgg caaaagatgg aataactgcc
    22021 atcactagtt ctgattcaag tgaagacagt tcagaaacta ctgatgcagg cggtgaacct
    22081 gctggaggaa tgggcgggaa tccgcaaatg actcaagcag actttcaaat cagtggtgtg
    22141 tcagcaactg cacaaaatag taatttttct gatggaacag caaaaatcac ggatggtgaa
    22201 ggaatagatg acagcgacaa aggcacaaac aatgtagtca ttgattctac tctagcagaa
    22261 acaaatgacc tggcagtcgg agatactttc acaatcacga gtactgaaga tgaagatacc
    22321 acttatgaaa tgacgatcaa aggaatttat gaatccagcg aaacaagcgg cagtatgggt
    22381 atgaaattca acttcatgaa tccgtcgaat acactttaca cttcttacac ttttgcaaat
    22441 gaactaaatg ggacatctga cgataacacg atagattctg ctatttacac tctatctgat
    22501 ccagataaaa tggatgagtt tctcaaagaa gctgagaagc tgatcgacac agatacattt
    22561 agcttacaat caaatgattc catgtatcag tcaatgttgg aacctttaaa caacgtggca
    22621 agtttttcta aaaatgttgt tttactagtg gctgtagccg gtattatcat acttactctg
    22681 atcattatga ttaccattcg ggagcgtcgg catgagttag gcgtcttgct atctttagga
    22741 gaatccagaa gtaaaatcat tctccaattg tttaccgaag tggctatttg tatgattctt
    22801 gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga atgccgtggg acagcagcta
    22861 cttgaccaac agacagaaac cacgacacaa aaccagcaag ctcctggcgg tggacaaatg
    22921 ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc aacaaggaac cacgaataat
    22981 ccatttgctg tttctgatca agtcagcgaa ttggaaatca atgtcaaacc tgcacaattg
    23041 ggactattag ccggagtagc atttggaatc agtctgtttt ctgtcttttt agcttctata
    23101 gggatattaa gattaaaccc aagaaaaatc ctattaaact aaaggaggaa tttgatgtta
    23161 cggacagaac aattgggatt ttggtatcaa gatccgcaag atgcgcttta taaagatgta
    23221 gaactgaatt tcgaagctgg aaaaatgtac gctattttag gagcaagtgg ttctggaaaa
    23281 acgacttttc tttcgcttat ttctggacta gatgcaccaa aagaaggaaa ggtatactat
    23341 caagatcgtt cactgaacaa aatcgggtta agaagttatc gtaaaaatga tgtctcgatt
    23401 attttccaag cttacaactt gcttccttat ttatcggctc tagacaacgt catagcagca
    23461 atggaaatca ctaagaccaa gcaagagaat tatcgacaag ttgctttaga tcgactggct
    23521 cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga ccaaactttc gggaggtcag
    23581 cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac atcaactaat cgtagcggat
    23641 gagcctactg gaaatctaga tgaaacaact tcacaagata tcgtcaaact tttccaagaa
    23701 atcgcccatg aacaaaatcg ctgtattatc atcgtgactc atgagattga agtagcaaaa
    23761 agctgtgatg aagtctttga actaaaaaat aaaaaatttc aaaaaatcga agtttaaaaa
    23821 acaggataat gatcaaataa aaaatccgaa ccatatctgt ctcgcagata tggttcggat
    23881 tttttatttg cttgattcaa ttagtaataa gtggttttta ttttttttcg tcttttcgag
    23941 aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt ttctttgatg tcagcaccta
    24001 gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc tgttgctttg ccgtatgctt
    24061 cttttccttc accgactact ttatctttca tatctttaga acgtcctttt aaatctgcca
    24121 ttgtgcttcc tcctcttagt ttgtttattg atctaccttc agggtaacat caacaacaga
    24181 tacagacaaa cagaacgctc gttttctaaa aaagatagtt agagctgttg attaaaaact
    24241 tttttcgaaa gtcccccata ctttgacttc tttttcttgg accattattt ctcctttagc
    24301 gataactgta tcaatatcaa gcgtttcttt atccaagata cacagatctg catcagcacc
    24361 aatttcgatt tttccttttt tatccaattt caatatacgt gctggattac tggttatagc
    24421 tcgtaaggcg atttccaaag ggatactttc tttttgcaca gcttctttga tccctactag
    24481 caatgccttg gcacttccta ctcctaaccc taggaaatga ttgttttcat caaaataagg
    24541 caagcttcct tgtccatctg aggtcatcgt gaaattatct aaagaaacat cttcctcgat
    24601 caatcgtttc agtgctttgc tgaagcgaac ttcaccatct gtcttctccc aaaaatcagg
    24661 atcttcgcta gcagtgaagt caatcgtgcc accacgtttg gcaaatgcca cgcaagcttc
    24721 aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat tgtgtgatcg gaatttctgt
    24781 ttcttctacg attcgtgtca atagctctag ctttcttttt cctccgccta agtggacatt
    24841 gatgatccca gcttttccgg ataacatgcc tcctacacga gcatctgcta ctgcacgagc
    24901 gaactcttca aaacttggct gagaagaacg gtgatcggat actgcgattt ctccgattcc
    24961 gataatttta tcaattgtca aaaagtcttt gatgatagaa tctgttactg tttttaccgg
    25021 caatcggtac gaaccttcgt atacataagt ggagattcct tctgcttcca gacctttcgc
    25081 tttgctgatc aacgcagtca tatctctacc ttctccgtct gttcccaaac aaccgacaac
    25141 tgtcgttaca ccagctgtag tcaattggct caatgtgact tctggcgttc gattttgata
    25201 accgccttct cctcctccac ctaaaatatg gaaatgacag tcaatgaaac ccggggttag
    25261 tacttttccc tcacctgaaa gttcttctac ttccactcct tcgtagccgc cttctagctg
    25321 ttcttctaca gccaaaatct tcccaccggc aatcagtaca tccatttttc ctaagtaatc
    25381 tggtgcaaac acttcaattt gtcgaatgat tttcatacat ttttcctctt tctttaagta
    25441 taactgatga taggatctta aaaaataatc caaacgatag gatctttact cctttgattt
    25501 taccgtaaaa tgacggttct tggagtcctt atctactgtt tggattactt ttttatttac
    25561 aggtctccag tactactgca gacctattgc aatggcaatc atgatgaaaa tagttcctaa
    25621 aacaaacagg aatccttgca tcttgatttg gaatcttgcc cattttgccc attcgatccg
    25681 tgcaacacct agcacaccaa tcaagctggc tgatacgggt gtaaaagcgt ccataaaacc
    25741 tgaaccaagc tgataagcaa gaactgctac ttgtctggat acacctacta aatctgccag
    25801 tggagccatg attggcatag tcaaagctgc ttgtcctgaa tttgatgtaa caaccaagtt
    25861 gaacaaactt tggaagatgt acatcgccca agcaccaatg acagctggaa caccggtcaa
    25921 cgccgtcccg atcccatgca aaatcgtatt caaagctgaa ggaacagatg catcagatcc
    25981 tcccaagaca agaagaatcc ctttggccat acctactacg attgctgtac cagctaaatc
    26041 agctgcacca gattggaaag aagaagcgat gtcattgact ttcattccat ctaatttgaa
    26101 aagaacagca atgattccag caactaatcc catcacaaag aattgagaag cgatttctgg
    26161 aatgtaatag cctttttgag tcacgcccca aacgatccag acaaggacaa ctaacatctc
    26221 taataagatc aatttatgcc caagcatgaa ttttttattt tcatcagatg tctttttgat
    26281 atgagaacgg aaataatcat ctgatttgta agtcaacgaa ctttctggtt ttttacggat
    26341 tttttctgca tatatcatca tataaccagc ggctaatgca gtaacaacta cccacataat
    26401 caatcggaat gtcgcaccag acaacacggg gattccagca atcccttgag ccaccgctac
    26461 gctaaacggg ctcatccaag aggtcgcatt tccgacttga gatgcgacat aagtcacggt
    26521 tactgctacg atcgagtcat atcctaacgc aatgacaaat ggaaccatca ccattgagaa
    26581 tggaattacc tcttctgcca tcccgaatgt agcgccaccg aatgaaaaag caaagaataa
    26641 tagcggtaaa gctaaacgct ccagtccctt agttttactg ataaacgcat agatacctgc
    26701 gtcgatcgct ccggtacgca taataatacc aaaagcacct ccaacaacaa ggatcaatgc
    26761 agcgatccct acagcagatc catacttatc tccagatact agtccttcaa aaacaaaatt
    26821 taagaaacca aagccgccaa aatcatctgt tccccatatt ttggctgttt tatgcaattt
    26881 tttagaagta tcatagatat tttcgccgta ctgatcgtaa agcacatcat ctgtcaagga
    26941 gatttcatct agtttttcct gcgtaagatt tttttctccg tcagcaatga ctttttccaa
    27001 tcctttttcc ggaacatcca gtttttcacg ttctgctgga tgatcttgca gttcttctaa
    27061 ttgatcaaac acatacgact tatccagctc gtaagcataa cggaaggaat cttgacgtaa
    27121 aacagtacga gtacttgttt cgccatttgc atctttgtat tcaatatctt gcgtgctgaa
    27181 ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag atgactacac caaaaataat
    27241 gacatacgta tggggtgtcg ataatttagt gaagtcgcgt ttcggttttc ctgaagaatt
    27301 tgttttcttc aaatttattt cttcctttct tttttcagta attttgatga ataaaaaatg
    27361 gataatcaaa tgaattatac agtaaaaatg acatttccac atgaataatt tattagaaaa
    27421 tcact
//
LOCUS       DACCZY010000027        26085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCZY010000027 DACCZY010000000
VERSION     DACCZY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.027"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(1237..1902)
                     /locus_tag="IVZ67_000945"
     CDS             complement(1237..1902)
                     /locus_tag="IVZ67_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165037.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..4064
                     /locus_tag="IVZ67_000946"
     CDS             2028..4064
                     /locus_tag="IVZ67_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8165038.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="IVZ67_000947"
     CDS             4068..4706
                     /locus_tag="IVZ67_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165039.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..5162
                     /locus_tag="IVZ67_000948"
     CDS             4734..5162
                     /locus_tag="IVZ67_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165040.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5619..6530
                     /locus_tag="IVZ67_000949"
     CDS             5619..6530
                     /locus_tag="IVZ67_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8165041.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6747..7184
                     /locus_tag="IVZ67_000950"
     CDS             6747..7184
                     /locus_tag="IVZ67_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165042.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7279..8163
                     /locus_tag="IVZ67_000951"
     CDS             7279..8163
                     /locus_tag="IVZ67_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8165043.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8387..11554
                     /locus_tag="IVZ67_000952"
     CDS             8387..11554
                     /locus_tag="IVZ67_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165044.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11574..12119
                     /locus_tag="IVZ67_000953"
     CDS             11574..12119
                     /locus_tag="IVZ67_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8165045.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12147..12602
                     /locus_tag="IVZ67_000954"
     CDS             12147..12602
                     /locus_tag="IVZ67_000954"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8165046.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12599..13357
                     /locus_tag="IVZ67_000955"
     CDS             12599..13357
                     /locus_tag="IVZ67_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8165047.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13443..14183)
                     /locus_tag="IVZ67_000956"
     CDS             complement(13443..14183)
                     /locus_tag="IVZ67_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8165048.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14244..15497
                     /locus_tag="IVZ67_000957"
     CDS             14244..15497
                     /locus_tag="IVZ67_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8165049.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15819..16046
                     /locus_tag="IVZ67_000958"
     CDS             15819..16046
                     /locus_tag="IVZ67_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8165050.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16169..16495
                     /locus_tag="IVZ67_000959"
     CDS             16169..16495
                     /locus_tag="IVZ67_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8165051.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16563..17276
                     /locus_tag="IVZ67_000960"
     CDS             16563..17276
                     /locus_tag="IVZ67_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8165052.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17586..18436
                     /locus_tag="IVZ67_000961"
                     /pseudo
     CDS             17586..18436
                     /locus_tag="IVZ67_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            18557..20422
                     /locus_tag="IVZ67_000962"
     CDS             18557..20422
                     /locus_tag="IVZ67_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8165053.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20470..21879
                     /locus_tag="IVZ67_000963"
     CDS             20470..21879
                     /locus_tag="IVZ67_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8165054.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21987..22688
                     /locus_tag="IVZ67_000964"
     CDS             21987..22688
                     /locus_tag="IVZ67_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8165055.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22847..23326
                     /locus_tag="IVZ67_000965"
                     /pseudo
     CDS             22847..23326
                     /locus_tag="IVZ67_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24174..24749
                     /locus_tag="IVZ67_000966"
     CDS             24174..24749
                     /locus_tag="IVZ67_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8165056.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24749..25189
                     /locus_tag="IVZ67_000967"
     CDS             24749..25189
                     /locus_tag="IVZ67_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8165057.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25205..25906
                     /locus_tag="IVZ67_000968"
     CDS             25205..25906
                     /locus_tag="IVZ67_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8165058.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         8805 a         4233 c         5377 g         7670 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     5161 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5221 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5281 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5341 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5401 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5461 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5521 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5581 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5641 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5701 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5761 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5821 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5881 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5941 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     6001 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     6061 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     6121 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6181 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6241 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6301 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6361 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6421 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6481 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6541 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     6601 aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt
     6661 acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat
     6721 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
     6781 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
     6841 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
     6901 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
     6961 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
     7021 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
     7081 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
     7141 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
     7201 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
     7261 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
     7321 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
     7381 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
     7441 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
     7501 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
     7561 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
     7621 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
     7681 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     7741 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     7801 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     7861 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     7921 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     7981 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     8041 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     8101 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     8161 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     8221 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     8281 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     8341 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     8401 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     8461 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     8521 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     8581 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     8641 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     8701 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     8761 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     8821 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     8881 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     8941 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     9001 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     9061 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     9121 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     9181 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     9241 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     9301 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     9361 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     9421 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     9481 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     9541 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     9601 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     9661 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     9721 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     9781 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     9841 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     9901 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     9961 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
    10021 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
    10081 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
    10141 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
    10201 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
    10261 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
    10321 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
    10381 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
    10441 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
    10501 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
    10561 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
    10621 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
    10681 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
    10741 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
    10801 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
    10861 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
    10921 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
    10981 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
    11041 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
    11101 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
    11161 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
    11221 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
    11281 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
    11341 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
    11401 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg
    11461 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
    11521 aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
    11581 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
    11641 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
    11701 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
    11761 aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
    11821 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
    11881 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
    11941 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
    12001 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
    12061 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
    12121 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
    12181 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
    12241 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
    12301 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
    12361 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
    12421 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
    12481 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
    12541 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
    12601 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
    12661 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
    12721 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
    12781 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
    12841 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
    12901 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
    12961 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
    13021 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
    13081 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
    13141 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
    13201 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
    13261 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
    13321 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga
    13381 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
    13441 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
    13501 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
    13561 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac
    13621 ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt
    13681 gttttgacca taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga
    13741 ggatacagga cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc
    13801 tgctgttctt tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg
    13861 tcttttctga tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc
    13921 gtataagcgg tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg
    13981 acatcaatac ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta
    14041 tcaatagtgc ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata
    14101 ttctcgatat gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct
    14161 tctatgataa aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg
    14221 aaacaaaaca atggaggaat aagatggtcg caacagtgac actttatttg acctttactt
    14281 tgattttaga tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct
    14341 ttggttgtat agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc
    14401 cttacctagt tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta
    14461 tcagctgttt gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt
    14521 ttgatctgat ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac
    14581 ttatcgcatt tattttctat ctattagtaa aaagtataga caaacagacg agatttctca
    14641 acttgctttt tacttatcca actgaacact attttggagg tatttgtatt ttattaagct
    14701 ggggcgtgat ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc
    14761 tatctctttt gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca
    14821 gtttccaagt aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag
    14881 aatattacca tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa
    14941 aaattactct tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc
    15001 attctgcagc tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag
    15061 ctgtcagggg tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc
    15121 aagtagttat tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct
    15181 gtttatctat ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta
    15241 cgatcaatct gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc
    15301 caacgaattt gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg
    15361 ggttaggtct atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa
    15421 tcgaattttc aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga
    15481 gcaaatcagc ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat
    15541 agagacaaca acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc
    15601 tgtttctttt gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta
    15661 cgaaagtata cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc
    15721 aaaatgctaa ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg
    15781 cgtggagaag caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag
    15841 agttagaaca agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag
    15901 agtactcgct caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt
    15961 gggaagaaga acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata
    16021 cacatggtgg ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac
    16081 aggagttttt ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag
    16141 taagaagtga ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag
    16201 agatgaacgt aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt
    16261 ggaagcatct gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac
    16321 acaattagaa cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc
    16381 agacaacctt cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt
    16441 gaaatatcct gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg
    16501 tgtcattcgg ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga
    16561 aaatgaaaaa aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt
    16621 tattagtagg actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga
    16681 caggagataa atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata
    16741 aagatgtcag ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt
    16801 cgcaaatgag aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa
    16861 tcaatttacc ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat
    16921 tatctccaga tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga
    16981 accctgaatt gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga
    17041 cagatttaga aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag
    17101 atacccaatt actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg
    17161 acatggacgc cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa
    17221 aagatgcgac agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata
    17281 ctgggacata agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa
    17341 ccaatttgct gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta
    17401 gatagacttg tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat
    17461 tcgatggtat gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag
    17521 aattcagcta agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa
    17581 gcaaaatgaa aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg
    17641 gggaagaagt cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga
    17701 tttcgccaga agcggtccaa aaagaatttg tttaagagat tcttttgcta aaaaccaatt
    17761 ccagcagctg atggctgata ttccggcaga ggagatcgaa tgggtcaaac aagtagtcga
    17821 actggctgaa gataaactaa aagttgaatt ttcgcctaat atttatctga cattgaccga
    17881 tcatcttcat tatgcgatca cacgagcaaa agaaaatatc caactgccta atccattatt
    17941 atttgaaaca aaaaaatttt atccgaaaga atatcaagca gcgaaagagg ctcttttgtt
    18001 cgtgaaacaa aagacgggtg tggaactttc tgaggatgaa gccggtttta ttgcctttca
    18061 ttttgtgaat agccaacaag gaaacgaaga catgcaagtg acgatgaccg ctaccacaat
    18121 ggtaagagat atactgagta tcatcagtaa attcttcggc atcgtgttcg atgaagaatc
    18181 tttgaattat cagcgtatca ttactcatct gcagtttttt gcccagcgtt atctcaaagg
    18241 ggaaggatct gacgagcaag atgaatttct ctacgcatta gttcaaggaa agtatccaaa
    18301 agcgtttcat tgtgtcgaac ggatcaatga gtatcttcga caaacgcaaa aacaggaaat
    18361 ggggatcgca gagcagattt atttaacgat tcatattcaa agagtagtaa gtgagaaaaa
    18421 aagcatccaa tcataaggat tgttactgta tggcaggcaa aacctggatt gaaaaaatag
    18481 gttcacgaat gtttgtgact gtttttttca atctgggttt ttatttttta aataaaaaag
    18541 aaagaaggaa aaaacaatgg ataatcaagc agttggtcgt cgtgtatggg aagcggtagg
    18601 aggacaaaag aatgtcaaaa gtttagtaca ttgtgctacg cggttgcgat tcagattaaa
    18661 agatgaatct ttagcagata cgcaaaagtt aaaagaagac ccggacgtga tccaagtcgt
    18721 acaaagtggc ggtcagtatc aagtagtgat tggcagtaat gtagcagatg tttatcaatc
    18781 gatcgtggat gaggaaggtc ttgcagtttc ggaggatgga aaagaagagt ccaaaaatat
    18841 tttgaatcgt ctgatcgata ttatctctag tatatttaca ccttttctag gggcgatggc
    18901 agccgccggg gtattgaaag gattcttatc attggcaacg gtgatgggct ggctgacagc
    18961 tgatagcggt acttatcaaa tattgtttgc tgcagcagat ggtgtcttta ccttcttacc
    19021 tgtcatgctt gcttttacag cagctaaaaa gttcaaagcc aaccagtttt tagcagtagc
    19081 aattgctatg gcacttgtgt atccagctat cacagcagtt gctggtgcgg gagaagctat
    19141 cagtttcttt gggatcccag tgattctttc accatctggt tatacttctt ctgtcatacc
    19201 aatcattcta gccgtatggg ttcaaagtaa attagagcca ttcgtaaaaa aagtgattcc
    19261 gcaatttctg caaatgattt tagttccgct tgtcgtttta gtagtgatgg ttccattaac
    19321 atttcttgct ttaggaccaa tcgggacggt tgccggtaat gctttaggcg gtctcttcaa
    19381 ctcgatttat ggatttagtc caatcgtagc aggtctaatc atgggaagtt tatggcaagt
    19441 attcgtgatg ttcggcatgc attggggatt cgtaccgatc atgtttttga atatcgaaca
    19501 atatgggttt gatgtattga tgccaatgct tcttccagcc atcttggcac aaggtggggc
    19561 tgctttagct gtagcattgc gaacaaaaga tacgaaactt cgagcattag ggatttcttc
    19621 tacagtgact tcgttatttg gaatcactga accaactgtg tatggtgtga cattaccttt
    19681 gaagaaacca ttcattgccg cttgtatttc tggaggtatt ggtggagcta ttatcggatt
    19741 ttccggagta aaagctttct ccagcagttt ggtcagctta ttgacgattc cgactttcat
    19801 taatacggtg gatggtgtag agtcaaatgt gacagtagca gtaatagcta caggaattgc
    19861 ttttgttctt gcattcgtag gtacattgat tttaggattt gacgaacaaa cacaagacaa
    19921 tcaattagaa aacaaacatg cgaatgcagg agaaccgatt acttcagcac ggcatacctt
    19981 aaaaagtcct ttaacgggta aagtactgcc attgagtgaa gtaccagacc aagttttttc
    20041 ttctggcgtg atgggaaaag gtatcgctat tgatccagaa gtaggagaac tagttgcacc
    20101 tgcagacgga gagatcacga ctatttttcc aactggacat gctgtaggga tcacaacgac
    20161 tgacggagcg gagattttga ttcatatcgg gatggataca gtagaattaa atgggaatgg
    20221 ctttgagatc ttagtcaaac aaggggatct cgtcaaagca ggagatttat tgattcgttt
    20281 tgacatcgaa gcaattagag cagctggcta tagcgtaatc actccagttg tcattacgaa
    20341 tactgatgca tttgccgata tacttgaatt agaccaaaaa gagattattg caaacgaaga
    20401 tgttctggca attgtaaaat aaggaggaaa tcaggatgca atcacgtttt tcagaaaaat
    20461 ttttatgggg tggagcaact gctgccaaat caattagaag gggcttatga tcgagacgga
    20521 aaaggactat ccgttgcaga tgctatgcct ggaggtaagc agcgctttgc gattatcggc
    20581 agtgaagaat ttgactggac gattgatcaa gaaaaatata tttacccgaa tcatcgagga
    20641 atcgatcatt acgatcgttt caaagaagat cttgccttgt ttgcggaaat gggattcaaa
    20701 tgttatcgtt tttcgattgc ctggacacga atttttccaa atggagacga aggtactcct
    20761 aacgaagcgg gattagaatt ttatgatcag ctgattgatg agtgcttaaa atatgatatt
    20821 gaacctgtga tcacgatttc tcattacgaa atgcctcttc atttggcaaa agaatacggt
    20881 ggctggaaaa atcgaaaact gatcgacttc tatgaacgat ttgctcagac agtattagag
    20941 agatacagca gtaaagtcaa atattggatg actttcaatg aaatcaattc ggcctttcac
    21001 tttccagctt taagtcaagg attagtaaaa agcaatggag caggagagta ccaaaatatt
    21061 ttccaagcat ggcataatca attcgttgca agtagcaaag ccgtaaaaat cggccacgaa
    21121 ttgcgttcgg atatccaaat cggctgcatg attatctatg caacaactta cagcatcgat
    21181 gccaatccag tcaatcaggc agcaacaatg atccaaaacc aagaattcaa ttttttctgc
    21241 acggatgtcc aagtacgtgg agaataccct gcatacacag cgcgtaccca taaaaaatat
    21301 ggagtcgatc cggaaaaatt agagcaaacg gaaaaagact tccagttgct aaaagaatat
    21361 ccagtggact acatcggatt tagttattat atgtctacgg caatcaatga gactgatcca
    21421 caagcagcaa cttcagaagg gaacttgctg ggaggagtaa aaaatccgtt tcttgaggca
    21481 agtgaatggg gctggcagat cgatccagaa gggctgagaa tcgcattgaa tgaattatac
    21541 aatcgttacc agaaaccatt attcattgtt gaaaacggtc ttggagccat tgatcatatg
    21601 gaagaggatg gcacgattat tgatgattat cggatcgatt atttacgtcg tcatatcgaa
    21661 gcgatggcta atgcagtagc agacggtgta gatttgatgg gctatacgcc ttggggatgt
    21721 atcgatcttg tcagtgcatc aacaggagaa atgagtaaac gttacggctt tatctatgtg
    21781 gatctagacg atgaaggaaa tgggacactg aatcgatcaa agaaaaaatc ttttgattgg
    21841 tataaagaag taattgcctc aaatggcgta aacttgtaat taaagcagct gaatgaaaaa
    21901 gaacacactt ctcacatgtg atttgtgttc tttttttatt ttttatgtgc tattctttta
    21961 gtaatagtaa agcaaagggt gacgttatga aaaggacacg tgtactatac ttggaagtcg
    22021 cagataaaat taaagaagat attttctcag gaaaatatcc agtcggttcg atgttgccga
    22081 cagaaagtga attagaagaa ctgtttcaag tcagcaaaat cactgtccga aaggcaatcg
    22141 aactgctggc atcagatgaa tatgtcgcaa agaaaagtgg tcgtgggacg acggttctaa
    22201 gcaatcgtcc ttacaatcga ttatccaaag cggcaagctt tacgcaaatc ttggagaact
    22261 cccgatatga agtccggaaa gaaacgctgg atttgcaatt ggtccatata aagaaggaag
    22321 atcctttgtt ttcctttttc ggtgagcaag ctgtttgttt taagcgctta tattatttga
    22381 acaatcagcc ttatatttat tttgttcatt atcttccaca agaaatgatg caattcagca
    22441 gagaagaatt tggcaaagaa tctttgtatc gtttgctgac gaaaaaagga tatactattg
    22501 actcattttc tgatgatttc tcggcagtat ttttaacaga agaagaacaa ggaattctga
    22561 aaacagacgc acagatcggc atcaaacgga tacggaaatc gtctacgcaa attggggaaa
    22621 tgattgaata ttcggaggcg gtctaccata cggagctgca tccttaccat attgattatg
    22681 aaacataaat aaaaaaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    22741 caaacaaaaa tagcttctca tatttttcgt ttggttaggc ttattaccgc agatacacgg
    22801 ctgtttctgg attaccattt attctggata agggggtgtg gcacgatttt cagaaggaat
    22861 ctatgaacca atcactgtat tgattttagc ttcgatggta accccattcg gcttgacgat
    22921 cgcttatttc ttagggaaaa tcattcgtaa aaatatactc aatcgtcaag aaatcgatac
    22981 attgaagacc gcttttccta tggggatctg tcagatcaca gagggatgtt tccctatcgt
    23041 attgaacgac ttggcacgca atgtcatagc aacgggagtc ggtgggacag taggtggcgg
    23101 attaagcatg ttttggggcg cagatagccg tattccggca tcgggaatgt ttgcagtcgc
    23161 tacgatgacc agaccatggg cattcattgg tgcattgtta gctggttctt ttgtcacagg
    23221 aataaccatt ttattattga aaaaaccagt agatccgaat gcagaaatca tccaagaaga
    23281 aaaagaggaa gaagacatat cttgggatga tttgacaatt agttaggaga ggcagaatgg
    23341 attacaagaa aaaagtacaa tagatgtacc ttgagacagg aatggtatga aagaatcaga
    23401 gttagaaaga atcgaatata cagattttgg tttaggagcg atcgaaactg tccagagcat
    23461 cggcaccctc cttgaaatga cgatgaagga aaaatgagaa cctttcgttg tcgaaagggt
    23521 ctggtatacc ttatgtcgaa ggaaaggaaa caaaaaaact ttcctgtact attccaaaaa
    23581 ggaaagaaag ttattataca gtaaaaaaag aaattattct tcatgctaga gatcagtaca
    23641 cgttcgagcc caatatcaaa cactagttcc aggcaggcgc aacaggtgcg gttatcactg
    23701 aattttcttc cagcagtgac gattcaagtg atatttttac aaatccagct atttatcgtt
    23761 aacaaaaaga tttttaggag aagctgatga acaaaaatga tcaaattttg atcgtttagt
    23821 tcgtcggctt ttttatttag agaaacatca tttatcagtg aaacataaca cgagaacaaa
    23881 atgatttttt cgttaataaa agaaacaagt atttgttgtt taaaataata cacgttattg
    23941 gcatatagga cgaaatccca gtatcttcta taagtaagtc atttcatatc gatgaaaaca
    24001 cgttatttat ttttgtttta tctataaaat ggctgaaaaa tgattttttt ctaaatacta
    24061 tatatagtgg cgtctatcct atataccact aaaaaactac aaaatctagt cttttatttt
    24121 aaaaaaacac gtgtagaatg aagatagact tttgagttag ggagtgtttt tttatgaaac
    24181 ttactgtttt ttgcggttcc cgttttggta ataaagaaag ttacaaattt attgcgcaaa
    24241 cgttaggaaa atacatggca caagaagaaa tcgaactggt ttacggtggt tcatactccg
    24301 ggattatggg agtgattagt aaaacagtac tggaaaataa cggaaaagtc acaggtatct
    24361 atccgaatgg gctttttgaa actgaattgc ctggaaaaga tgttactaca tttattccta
    24421 cagaaacgat cgatgaaaga aaggtattat tatttgaaaa gggagatgct gtcctcgttt
    24481 tccctggagg tctaggtaca ttggaggaat tctcacaact tctttcttgg atagcaatcg
    24541 gtctgacgcc cgacaagcca atcggaatat tgaatattgg cggttattac agcggcttgc
    24601 aacaattatt agaaactttt gcaaaagagg attttatgga taaaaaatgg ttggatcaag
    24661 taatcttttc caacaatcca ttggaactgg tcaatgaatt acgagcagta tctattaaaa
    24721 atcagttatt attgaaggag gcaaaataat gagtcaaata tatttagcag gaccattttt
    24781 ttcagaggag caaatcgatc gagtaagtcg tatcgaaaaa gcgttggagg aaaacaaaac
    24841 agtcacaagt ttctactctc cacgtcatca ccaagaaagc aattatgaac tattcagcgc
    24901 tggatgggcg caggaagtat acgaaaaaga tatggaagaa ctgacaaatg cagaatttgt
    24961 tgttgctatc cttgactttg agcatcaaac aatcgatcca ggaacggctt acgaattagg
    25021 tgtggcaact atgttgaaaa aaccgatgat cattgtacaa gaagagacgg tacctacgaa
    25081 cttgatgatt acacaaagtc tccatacgta tttaaaatca gaccaagctg tacgtgaata
    25141 tgattttgaa acattgcctg ttgaaactta cgtaggcgag tacttataga agaggaaggg
    25201 ttgtatgtca gtgattttga tttcaggaac gattggtgca ggtaagagca gtttgacgga
    25261 tatgcttgca aaagagattg attctaaacc attttatgaa aatgtcgaag ataacgaagt
    25321 attgccgctc ttttattcaa atcctgagca gtacgctttt ttattgcaaa tcttcttttt
    25381 gaacaagcga tttttagcaa tgaaaaatgc tttagtgaac gatgacaatg tgctagaccg
    25441 atccatctat gaagatagcc tgttgttcca tctaaacgct gatttaggtc gggtaacgga
    25501 tatcgaagta cagcagtacg atagcttatt agatacgatg ctaaacgagt tagatgatgt
    25561 tgctccaaaa aaacggcctg atttgatggt ccatatcaaa gtttctttgg acacgatgct
    25621 agaacgtatc aaaaaacgtg ggcgtgacta tgaacagcta gaaagcgatg agacgttata
    25681 tacctattat gaaacgttga acacccgcta taatcaatgg tatgaagatt ttgatgtatg
    25741 tcctaaaata caagtggatg gggataaatt cgattttgta gaaaatccag aagatgcaaa
    25801 aaaggtgatc cgtcagatcc aagaaaaatt agctgaattg gaaggaaagc ctgctggaaa
    25861 atattttacg gataaccagc atgcaaaagg tcaagaatta ggctaaaaat aaaaaaccaa
    25921 acgacgggaa agaaaaatta ctacgtgatg tagaataagc ctttctatcg tttggttatt
    25981 ttatattcaa atatttgaaa taaataaatc tttactcttt gatttgtaaa ctgaaatgat
    26041 ccactgtttg ttttactgat gaagcttgat caaaggaaga agctg
//
LOCUS       DACCZY010000028        25931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCZY010000028 DACCZY010000000
VERSION     DACCZY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.028"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            55..1380
                     /gene="ftsA"
                     /locus_tag="IVZ67_000969"
     CDS             55..1380
                     /gene="ftsA"
                     /locus_tag="IVZ67_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8165059.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            1403..2644
                     /gene="ftsZ"
                     /locus_tag="IVZ67_000970"
     CDS             1403..2644
                     /gene="ftsZ"
                     /locus_tag="IVZ67_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8165060.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            2661..3338
                     /locus_tag="IVZ67_000971"
     CDS             2661..3338
                     /locus_tag="IVZ67_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8165061.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            3352..3951
                     /locus_tag="IVZ67_000972"
     CDS             3352..3951
                     /locus_tag="IVZ67_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8165062.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            3966..4253
                     /locus_tag="IVZ67_000973"
     CDS             3966..4253
                     /locus_tag="IVZ67_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8165063.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            4302..5084
                     /locus_tag="IVZ67_000974"
     CDS             4302..5084
                     /locus_tag="IVZ67_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8165064.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            5105..5812
                     /locus_tag="IVZ67_000975"
     CDS             5105..5812
                     /locus_tag="IVZ67_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8165065.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            6074..8872
                     /gene="ileS"
                     /locus_tag="IVZ67_000976"
     CDS             6074..8872
                     /gene="ileS"
                     /locus_tag="IVZ67_000976"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8165066.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(8921..10447)
                     /locus_tag="IVZ67_000977"
     CDS             complement(8921..10447)
                     /locus_tag="IVZ67_000977"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8165067.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(10462..11109)
                     /locus_tag="IVZ67_000978"
     CDS             complement(10462..11109)
                     /locus_tag="IVZ67_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8165068.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(11293..11622)
                     /locus_tag="IVZ67_000979"
     CDS             complement(11293..11622)
                     /locus_tag="IVZ67_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8165069.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            11799..12527
                     /gene="sfsA"
                     /locus_tag="IVZ67_000980"
     CDS             11799..12527
                     /gene="sfsA"
                     /locus_tag="IVZ67_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8165070.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            12543..13556
                     /locus_tag="IVZ67_000981"
     CDS             12543..13556
                     /locus_tag="IVZ67_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8165071.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            13556..14833
                     /locus_tag="IVZ67_000982"
     CDS             13556..14833
                     /locus_tag="IVZ67_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8165072.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            14896..17598
                     /locus_tag="IVZ67_000983"
     CDS             14896..17598
                     /locus_tag="IVZ67_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8165073.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            17750..18067
                     /locus_tag="IVZ67_000984"
     CDS             17750..18067
                     /locus_tag="IVZ67_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165074.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            18097..18417
                     /locus_tag="IVZ67_000985"
     CDS             18097..18417
                     /locus_tag="IVZ67_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8165075.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            18525..19985
                     /locus_tag="IVZ67_000986"
     CDS             18525..19985
                     /locus_tag="IVZ67_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8165076.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            20053..20274
                     /locus_tag="IVZ67_000987"
     CDS             20053..20274
                     /locus_tag="IVZ67_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165077.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            20305..20487
                     /locus_tag="IVZ67_000988"
     CDS             20305..20487
                     /locus_tag="IVZ67_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8165078.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            20487..20900
                     /locus_tag="IVZ67_000989"
     CDS             20487..20900
                     /locus_tag="IVZ67_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8165079.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            21023..22204
                     /locus_tag="IVZ67_000990"
     CDS             21023..22204
                     /locus_tag="IVZ67_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165080.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            22593..22666
                     /locus_tag="IVZ67_000991"
     tRNA            22593..22666
                     /locus_tag="IVZ67_000991"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22627..22629,aa:Arg,seq:cct)
     gene            complement(22735..23874)
                     /locus_tag="IVZ67_000992"
     CDS             complement(22735..23874)
                     /locus_tag="IVZ67_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8165081.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(24173..24808)
                     /locus_tag="IVZ67_000993"
     CDS             complement(24173..24808)
                     /locus_tag="IVZ67_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165082.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(24921..25556)
                     /locus_tag="IVZ67_000994"
     CDS             complement(24921..25556)
                     /locus_tag="IVZ67_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8165083.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(25590..>25931)
                     /locus_tag="IVZ67_000995"
     CDS             complement(25590..>25931)
                     /locus_tag="IVZ67_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165084.1"
                     /translation="KTIPFKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDE
                     NTVMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEE
                     FDFKKKELLGL"
BASE COUNT         8375 a         4468 c         5310 g         7778 t
ORIGIN      
        1 ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca
       61 aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct
      121 gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt
      181 aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga
      241 caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac
      301 atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc
      361 accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa
      421 cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa
      481 gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct
      541 aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa
      601 atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt
      661 ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag
      721 caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct
      781 attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca
      841 tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa
      901 ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt
      961 ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc
     1021 gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc
     1081 ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca
     1141 aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa
     1201 attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca
     1261 aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa
     1321 gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa
     1381 ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc
     1441 agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga
     1501 agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa
     1561 ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg
     1621 ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc
     1681 tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg
     1741 tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt
     1801 gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc
     1861 tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt
     1921 tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca
     1981 aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc
     2041 tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag
     2101 tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac
     2161 atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt
     2221 gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat
     2281 cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc
     2341 aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca
     2401 aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc
     2461 agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag
     2521 agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga
     2581 agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa
     2641 ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc
     2701 aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa
     2761 aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag
     2821 aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa
     2881 agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg
     2941 attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga
     3001 aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt
     3061 caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg
     3121 ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg
     3181 gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta
     3241 gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat
     3301 ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt
     3361 ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac
     3421 caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag
     3481 aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact
     3541 tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta
     3601 catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa
     3661 cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct
     3721 atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta
     3781 gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat
     3841 ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt
     3901 gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa
     3961 ctacgattta tactattgca ctattacttc atttacttca acgggcagcc tatttctata
     4021 gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc
     4081 taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt
     4141 tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg
     4201 tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc
     4261 aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc
     4321 agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg
     4381 ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt
     4441 tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac
     4501 aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt
     4561 ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga
     4621 aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct
     4681 cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac
     4741 aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga
     4801 ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca
     4861 gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg
     4921 gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag
     4981 atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga
     5041 aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt
     5101 actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg
     5161 atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt
     5221 gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta
     5281 cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga
     5341 taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa
     5401 agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga
     5461 agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga
     5521 aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga
     5581 gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag
     5641 ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga
     5701 aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga
     5761 tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa
     5821 agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg
     5881 gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga
     5941 cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc
     6001 acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg
     6061 agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat
     6121 gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat
     6181 ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc
     6241 tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat
     6301 tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac
     6361 acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc
     6421 catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg
     6481 agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga
     6541 tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat
     6601 ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc
     6661 tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga
     6721 cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac
     6781 attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc
     6841 cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg
     6901 ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc
     6961 aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga
     7021 cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg
     7081 tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga
     7141 agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt
     7201 aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg
     7261 gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa
     7321 attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa
     7381 aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc
     7441 atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga
     7501 aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa
     7561 agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt
     7621 tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt
     7681 tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca
     7741 atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc
     7801 atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa
     7861 atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct
     7921 tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt
     7981 aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa
     8041 tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga
     8101 caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga
     8161 caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc
     8221 gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca
     8281 acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac
     8341 tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta
     8401 cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga
     8461 tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag
     8521 aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca
     8581 agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc
     8641 agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga
     8701 tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt
     8761 tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat
     8821 cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa
     8881 agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac
     8941 gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt
     9001 cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt
     9061 ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat
     9121 gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg
     9181 ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa
     9241 atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat
     9301 agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt
     9361 tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca
     9421 aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga
     9481 atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat
     9541 agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt
     9601 ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac
     9661 ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta
     9721 aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat
     9781 cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc
     9841 attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt
     9901 ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct
     9961 ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat
    10021 tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa
    10081 attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta
    10141 atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt
    10201 gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga
    10261 ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga
    10321 ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca
    10381 atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat
    10441 tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa
    10501 gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat
    10561 gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa
    10621 ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa
    10681 tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg
    10741 acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac
    10801 atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac
    10861 taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct
    10921 tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt
    10981 agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt
    11041 gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt
    11101 tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa
    11161 aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa
    11221 caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac
    11281 attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata
    11341 aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa
    11401 acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt
    11461 gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc
    11521 ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa
    11581 acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc
    11641 actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg
    11701 aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat
    11761 tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa
    11821 tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag
    11881 aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg
    11941 ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg
    12001 ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa
    12061 ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat
    12121 acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg
    12181 gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt
    12241 ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac
    12301 aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa
    12361 cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg
    12421 gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg
    12481 ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa
    12541 taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag
    12601 ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac
    12661 aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt
    12721 tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc
    12781 aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga
    12841 cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg
    12901 aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa
    12961 aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag
    13021 tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg
    13081 atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc
    13141 attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt
    13201 atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag
    13261 agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac
    13321 aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc
    13381 caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt
    13441 ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg
    13501 atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa
    13561 cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat
    13621 ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt
    13681 agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa
    13741 cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat
    13801 tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt
    13861 ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt
    13921 gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca
    13981 agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat
    14041 cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac
    14101 agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga
    14161 cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat
    14221 acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat
    14281 tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt
    14341 accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca
    14401 attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga
    14461 tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt
    14521 gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca
    14581 agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga
    14641 gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc
    14701 tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa
    14761 caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc
    14821 aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc
    14881 cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac
    14941 caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca
    15001 agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca
    15061 gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct
    15121 acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag
    15181 caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa
    15241 actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag
    15301 atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc tcaaaatccg
    15361 caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa
    15421 gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac
    15481 cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt
    15541 ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact
    15601 gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg
    15661 ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa
    15721 caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc
    15781 atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt
    15841 gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa
    15901 gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc
    15961 gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt
    16021 gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc
    16081 acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat
    16141 gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat
    16201 cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg
    16261 ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca
    16321 ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa
    16381 aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa
    16441 caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta
    16501 ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga
    16561 cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa
    16621 gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat
    16681 gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca
    16741 ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg
    16801 atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta
    16861 gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa
    16921 gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt
    16981 gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta
    17041 gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta
    17101 ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc
    17161 ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata
    17221 gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat
    17281 gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc
    17341 gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc
    17401 atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc
    17461 tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga
    17521 aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa
    17581 aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg
    17641 cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca
    17701 tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa
    17761 aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca
    17821 aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc
    17881 agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt
    17941 catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa
    18001 tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat
    18061 gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa
    18121 gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt
    18181 gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa
    18241 tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat
    18301 catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg
    18361 tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata
    18421 caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa
    18481 ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa
    18541 gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag
    18601 ttgctttgcg tgatgctttt atcggcatac tgcccgcaac aatggcggga tcagtcgctg
    18661 taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca
    18721 gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt
    18781 ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata
    18841 atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct
    18901 tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt
    18961 caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac
    19021 tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta
    19081 cagttatgat catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca
    19141 ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt
    19201 taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag
    19261 ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca ttcctaggat
    19321 tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct
    19381 ttggtattca tggatcaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac
    19441 aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg
    19501 tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt
    19561 ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct
    19621 ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt atcttcaata
    19681 tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat
    19741 tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag
    19801 tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag
    19861 caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct
    19921 tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac
    19981 aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcga
    20041 ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg
    20101 ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag
    20161 ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta
    20221 atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg
    20281 gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt
    20341 tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg
    20401 gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga
    20461 gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat
    20521 cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca
    20581 aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata
    20641 accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat
    20701 cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct
    20761 gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata
    20821 tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata
    20881 tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa
    20941 gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt
    21001 ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat
    21061 tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg
    21121 agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga
    21181 tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta
    21241 cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat
    21301 caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta
    21361 tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa
    21421 aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat
    21481 aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc
    21541 ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca
    21601 aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat
    21661 cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga
    21721 tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga
    21781 atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc
    21841 tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa
    21901 ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc
    21961 gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa
    22021 agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt
    22081 attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga
    22141 atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt
    22201 ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca
    22261 tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct
    22321 tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga
    22381 ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt
    22441 ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt taaggactag
    22501 tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa
    22561 aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa
    22621 tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtata aataacccct
    22681 gtatcctttg cggtacaggg gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt
    22741 atcattttta tataaactat cgaacacagt attgattttt tctttatcct gatcttcaag
    22801 ctctcgaaca atatgaagat aagtagacat tgtagtttct aaactgctat gtcctaaacg
    22861 tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat gtgtatgcct
    22921 tagtccatgc aaagtaaatt gcttttcaat ccctaattct tttaattttt tgcgcaaata
    22981 tttgctgacg gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa caaaattatg
    23041 agggtttttg atttcaaaat tctcgaataa ttctttttga ttgattttaa acttttttaa
    23101 gagatcaatc gtatgtttat caatggaaat tttcctattt gaagtttcgt tttttgtttt
    23161 tccccattca tttaatttat aattccatgt tttattaata gtgatgattt gatcttcgaa
    23221 atcgatatct tcccatgtta atcctagaag ttcagcaaat cgcattccag tggcaccagc
    23281 aactaacacg agcataggag aagagtactg cgcagaaagg ttttcttcta cgagtttcat
    23341 taaagattta aattgatcat aatctaaata tttatcttct tttggcttca atgaatcttt
    23401 tcctttgatt actgcttttc tagttggatc aaacggaatt aatccttctt ctacggcatc
    23461 tttcaacgac gctctaatat ggttattaaa cttaataacg gtagatttta catggtcttt
    23521 agcatactta tttaaaaagc gttgatatcc cactctatct aaatcagaaa tcaataccgc
    23581 tggcatatat ttctttatgt tcattaacgt atcttcgtat tttctataag tgataggaga
    23641 tactgttcct tctttataaa gttgcatcca atcctcaaag taatctgata ataatagatt
    23701 tttgcgatcc cctttaaggc ctttgttcaa gttatattct aattcgttgg cggcatcttt
    23761 tgcttctcct tttgtcttaa atcctgattt tcttatcttc gtgtattttc cgtcatcttt
    23821 tttgtatgaa atttcgtatt gccaactatt tccacgtttg actaatcttg ccataattga
    23881 atcagctctc tttctctgat acaataggaa ctataaagaa gcccattgtt tgggttttat
    23941 tattttttgc acatcctttt tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg
    24001 gtataattgg atgtgtatta gatcatccaa tcagaggtgt ttattatgat taaagtgatt
    24061 tttaaaagaa taactgaact tccagagttt agattatttg ttgattattg gtatgtctgg
    24121 ttttttattg ttgcattgct gataggtcta gatgtcatat tagaaagaaa acttaagtaa
    24181 gagttgagtt aataaattga attcctaaat gagatatttt gtagcccata ttttgtttgc
    24241 cgacatcacc taatactatg gagctaatcg ttttttctaa cagaccaaaa tcagataatc
    24301 tatttaagct accatctatt agctcttgtg aatagccaga catgctgata tttccagtga
    24361 aattttcgtt tccacgactc attaagcatg ctaacaattg aatctcaatc gatgttaatt
    24421 gattcagtac aagaagataa tactcctcgt tatcccatga agcgttgtta attcgaagta
    24481 aacaattctt ataaagattt ttatacatat ctattttatt ttgtgccttt gatttttcta
    24541 cctcgtcatt tatcccttcg aatattccaa tgagttggtc cttgttctct aagttgaaaa
    24601 catcttctgg aatttgatcg cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt
    24661 tctcgatgcg tttgaaacgc ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc
    24721 caccaatata aggaattgct ccaataccgc cttcaactat taattcaaga tattcttttt
    24781 tggttagttt ttcattgcta ttatccatag ttacctccta ttttgtcatt tcctttacaa
    24841 gcattgtgac tcctgatggc actcttttgt ataggtttta ttattgtgca caatctaact
    24901 cttagtgggg cgtggttcat ttatttttta cttactttac tattaatcat atcccattga
    24961 gcataggatt tttctatgtt cgatcctctt gaactaattt ctcctgtaaa agttacttta
    25021 tcgccctttg taaaggtatc agcattgttt aagtccttcg caaaaattac atatgaaact
    25081 ttaggtgttg cagaaatatc attccaactt ttatcatcgt actctttgtc tgctataatg
    25141 gctattctag tagtcattga ttcgataaca gttccttcaa aagtatatgt tttaccacta
    25201 atatatttag taaaagctgt attttgctca gtagctggag taatagaata ataactatct
    25261 aagaaagcac cccagtcatg attatttgtc tctaaaaatt cttcaaattg agttaggctt
    25321 ttattgctgc tgttagatgt agtagtttga gtatcagatg atgagctttc ctgtttgtta
    25381 ttgtcatttg cctcgtttga tgaggaaagc gagaaaccta cggtagtaaa aataatcccg
    25441 ataataagga gaatcatagg tgttttctta tttgtttttt tgataaatct tacaattaac
    25501 atgattaaac ctataataaa taaaacaata ccagtaaaac caaatatacc gtccattaat
    25561 agatcctctt tctcttttga tattgaatat tataaaccaa gcaattcttt tttcttaaaa
    25621 tcgaattctt cttgattaat aatcccttca tctaacaact ctttatactt tttaatttca
    25681 tctgcacttg aaatttgtgt ttctttggaa ttattatcta atatagattg ttgcttctct
    25741 atatattttt ttaaattttc catttcattc caatattttt ttgtaaacat aacggtgttt
    25801 tcatctttcg tggccgctaa tatacctccg cgactttcgt ttccgcctag caaggtaaat
    25861 tgtatataac cattactcat acctggtttt tttaattgta cggctgatat atttttaaag
    25921 gggatagttt t
//
LOCUS       DACCZY010000029        25596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCZY010000029 DACCZY010000000
VERSION     DACCZY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.029"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..468
                     /locus_tag="IVZ67_000996"
     CDS             <1..468
                     /locus_tag="IVZ67_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8165085.1"
                     /translation="KKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLFLK
                     DKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQI
                     PIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            505..1515
                     /locus_tag="IVZ67_000997"
     CDS             505..1515
                     /locus_tag="IVZ67_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8165086.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1630..2343)
                     /locus_tag="IVZ67_000998"
     CDS             complement(1630..2343)
                     /locus_tag="IVZ67_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165087.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2533..3123)
                     /locus_tag="IVZ67_000999"
     CDS             complement(2533..3123)
                     /locus_tag="IVZ67_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8165088.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3607..3834
                     /locus_tag="IVZ67_001000"
     CDS             3607..3834
                     /locus_tag="IVZ67_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8165089.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3850..5016
                     /locus_tag="IVZ67_001001"
     CDS             3850..5016
                     /locus_tag="IVZ67_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8165090.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5250..6293
                     /gene="hrcA"
                     /locus_tag="IVZ67_001002"
     CDS             5250..6293
                     /gene="hrcA"
                     /locus_tag="IVZ67_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8165091.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6387..6950
                     /gene="grpE"
                     /locus_tag="IVZ67_001003"
     CDS             6387..6950
                     /gene="grpE"
                     /locus_tag="IVZ67_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8165092.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7001..8830
                     /gene="dnaK"
                     /locus_tag="IVZ67_001004"
     CDS             7001..8830
                     /gene="dnaK"
                     /locus_tag="IVZ67_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8165093.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8981..10147
                     /gene="dnaJ"
                     /locus_tag="IVZ67_001005"
     CDS             8981..10147
                     /gene="dnaJ"
                     /locus_tag="IVZ67_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8165094.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10229..10894)
                     /locus_tag="IVZ67_001006"
     CDS             complement(10229..10894)
                     /locus_tag="IVZ67_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8165095.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10870..11043
                     /locus_tag="IVZ67_001007"
     CDS             10870..11043
                     /locus_tag="IVZ67_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8165096.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11088..11825)
                     /locus_tag="IVZ67_001008"
     CDS             complement(11088..11825)
                     /locus_tag="IVZ67_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8165097.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11827..13329)
                     /locus_tag="IVZ67_001009"
     CDS             complement(11827..13329)
                     /locus_tag="IVZ67_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8165098.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13588..15582
                     /gene="uvrB"
                     /locus_tag="IVZ67_001010"
     CDS             13588..15582
                     /gene="uvrB"
                     /locus_tag="IVZ67_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8165099.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15592..18408
                     /gene="uvrA"
                     /locus_tag="IVZ67_001011"
     CDS             15592..18408
                     /gene="uvrA"
                     /locus_tag="IVZ67_001011"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8165100.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18671..19936
                     /locus_tag="IVZ67_001012"
     CDS             18671..19936
                     /locus_tag="IVZ67_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8165101.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19940..20671
                     /locus_tag="IVZ67_001013"
     CDS             19940..20671
                     /locus_tag="IVZ67_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8165102.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20806..21690
                     /gene="rapZ"
                     /locus_tag="IVZ67_001014"
     CDS             20806..21690
                     /gene="rapZ"
                     /locus_tag="IVZ67_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8165103.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21687..22685
                     /locus_tag="IVZ67_001015"
     CDS             21687..22685
                     /locus_tag="IVZ67_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8165104.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22719..23654
                     /gene="whiA"
                     /locus_tag="IVZ67_001016"
     CDS             22719..23654
                     /gene="whiA"
                     /locus_tag="IVZ67_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8165105.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24197..24847
                     /locus_tag="IVZ67_001017"
     CDS             24197..24847
                     /locus_tag="IVZ67_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165106.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25034..>25596
                     /locus_tag="IVZ67_001018"
     CDS             25034..>25596
                     /locus_tag="IVZ67_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8165107.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8443 a         4337 c         5475 g         7341 t
ORIGIN      
        1 aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca
       61 aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc
      121 ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa
      181 acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa
      241 cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa
      301 cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca
      361 ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta
      421 cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata
      481 gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag aaatgcaacg
      541 attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca
      601 gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg
      661 caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg
      721 caagatgaaa atatccaaat gatctttccg acttggggag gagaattatt gattgagatt
      781 ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt
      841 agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca tggaactaac
      901 cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg
      961 aaaacaagag aaggagaatc gattctacag caatcttctg aaaagtatca aaaagtgtgg
     1021 ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca
     1081 gtcagcggag atgcagtaag gatgaaggga cgattattag gtggttgtat tgatacgatc
     1141 aggcatttaa tcggtactcc ttatggagat gtccaatctt ttaaaagaaa gtataatgat
     1201 ggaaagccga tcttgtggtt tctagaaaac tgtgagctat cgtcagttga tttaagaaga
     1261 tcacttgttc agatgaaact ggcaggctgg tttgatggca gctcggggat catgttcggt
     1321 agaagtgcgg cagaggattt gttagattat aaaatgaagg atgtatacga agatctgtca
     1381 aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg
     1441 acattgatta atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg
     1501 caaacgttta tttaaaaaat tttttcctgc cttggagtct atcaaagacc aataaatcta
     1561 aatagaaaag aaagtgaaag aagctgacaa aatgctgtca gcttcttttt gtataacata
     1621 ttagaaagtc taattatcta taactgtcac atcgattaac tgtttcacgt cattgggatt
     1681 tcctgtagga atagctacta cagatgcatt atcaatgatt tcttgagttg tttctctaat
     1741 gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa ctggataact
     1801 agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc
     1861 agtgagcata accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg
     1921 tgttcctgca ggataatgaa taacatgtaa ttcgaaatca cccgatgtat ctctaaaagt
     1981 attacctcta atagctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc
     2041 taacggatct attgactgag aagactgtga ggaggttgtg tctattgttg aagaagaaat
     2101 atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct
     2161 tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga
     2221 actggtttgt ctgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact
     2281 aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac ctaacttttt
     2341 catataaaat acctcattat ttttttaata ccagtatagc ataatcctga atttttgaat
     2401 ccgtaaaacc ttgtctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
     2461 agtgtgacaa ataatcatca gtaaaattac ttgtcacact attgataagg agtatttaat
     2521 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
     2581 tttgtataga cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
     2641 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
     2701 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
     2761 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
     2821 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
     2881 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
     2941 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
     3001 gacctctgat tggatagaag cagttttgtt ttgccatttc tcattttggt ttagtgtgct
     3061 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
     3121 cattttcaag ctctccttta ctatctataa caaggacaac agttttaacc tacagacatt
     3181 cattaaaatt gggtagcgac agcgaaaaca acaattctta agttttagac agagtataca
     3241 gatagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     3301 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     3361 acaatattaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     3421 cgggcactca gacatgaaaa caaccctaga gatatataac taggtaacca gtttaatgaa
     3481 agaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     3541 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     3601 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     3661 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     3721 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     3781 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     3841 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     3901 atcccatttt gcgagcatat ctgctattac tgtgatttta ataaagtttt tttagaaggg
     3961 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat gctagaaagt
     4021 catccgacag cgaatttaga aacactttac gtagggggcg ggacaccaac ttctttatct
     4081 gctaaacaac ttgatcgact gttatcaggt gctagagaga tcctgccttt tcaaaatggt
     4141 aatgagttta ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact tcaagtaatg
     4201 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgataa tcgcttattg
     4261 aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc aaactatgca atttttagaa
     4321 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     4381 gaaggatatc tggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     4441 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     4501 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     4561 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     4621 cataacctca tgtactggaa caatgcccac tattttgggt ttggagctgg tgctagcggt
     4681 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     4741 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     4801 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     4861 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     4921 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ctttttgggg
     4981 aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta aagaatattt gttttttatc
     5041 tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac tattgaaacg ttgatataac
     5101 tggatttaca tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag
     5161 tctttctctt gacaggagaa gggcttcttg ctatatttat aagtggatta gcacttagaa
     5221 tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac
     5281 gtctgatcat ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattgatgt
     5341 ctgagggagt caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg
     5401 aaggattgct gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc
     5461 gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga
     5521 ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg
     5581 agatattatc tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc
     5641 gccgactgac tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg
     5701 taacagatat ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc
     5761 aggaactaga aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga
     5821 cggtgtacca taaacttcgt acggagattc cgatgattct tcataaatat ttccaaacga
     5881 cagaagggat gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gataaggttt
     5941 atgtcagtgg acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca
     6001 aatcgatgtt ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg
     6061 atagcaatat tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga
     6121 gtatgattca agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag
     6181 ggcctactag tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac
     6241 tagcagctaa acttgctgat tactaccgtt cgcttgactt gtcaaactcg taatgtgaat
     6301 aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga
     6361 ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa
     6421 tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt
     6481 cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg
     6541 aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg
     6601 agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa
     6661 ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga
     6721 aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg
     6781 aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg
     6841 ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca
     6901 aattacatga tcgcgtgtta agaccaacca tggtcattgt tgcacaataa ttgtgcggat
     6961 ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat
     7021 tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat
     7081 cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat
     7141 ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat
     7201 caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc
     7261 acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg
     7321 tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca
     7381 agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc
     7441 aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt
     7501 attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt
     7561 tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat
     7621 catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa
     7681 aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt
     7741 atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt
     7801 ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac
     7861 aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag aaatcgacga
     7921 agttatttta gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga
     7981 aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc
     8041 tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc
     8101 attgtcatta ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac
     8161 aacgattcca acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt
     8221 agacatccat gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag
     8281 attccaattg acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt
     8341 cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga
     8401 acaaaaaatc acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt
     8461 aaaagatgca gaagcaaatg cagaagcaga taaagctcgt aaagaggaag ttgacctacg
     8521 taatgatgta gatgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa
     8581 agtggacgaa gaagaagtga aaaaagcaga aacagctcgc gatgaattga aagccgctgt
     8641 tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat gcattgaacg aaatcgttca
     8701 aaacttgact gttaaactat acgaacaagc ggcacaacaa caagctcaag aaaacccaga
     8761 agcagctcaa ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga
     8821 cgataaataa tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt
     8881 cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca
     8941 agcgacgtga aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta
     9001 ttatgaagtc ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg
     9061 taagctttcg aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt
     9121 caaagaagta tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatatga
     9181 ccaatacggg catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt
     9241 tggtggattc ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc
     9301 tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt
     9361 gcaatatacg gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa
     9421 gtataatcgg gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac caggcacaca
     9481 accaactact tgtcataaat gtcatggtgc aggtacgatt aatgtagaac gccaaacacc
     9541 acttggacga gtgatgagcc gtcaaacttg tgatgtttgt catgggacag ggaaagaaat
     9601 caaagaacct tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa
     9661 agtgaatgta ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga
     9721 agctggagaa aacggcggac catttggtga tttgtatgtg gtcttccgtg tagaagaaag
     9781 tgatatcttt gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca
     9841 agcagctttg ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat
     9901 ccctgctgga acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt
     9961 acgtggtggt gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct
    10021 aaatgaacaa caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga
    10081 agaacagcaa gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg
    10141 gaagtagttt ttctagtaaa aacgctaagt cattaataat taaaaaccag acgaacaatc
    10201 atatgttcgc ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa gccagcttta
    10261 gaatatgata atcgtcatag tttctagggg tataaccagt gagagaatgg aacttgttca
    10321 atcgatattg caaggtgttt ttatgaataa ataattcttc tgagcaattt ttgatgcttc
    10381 cgttattttt actgaagcat gccaaagtct tcttgaacaa atgtacttct tcttctgtca
    10441 ggcttttcaa aatacgtttt acatataatg ttcgttgatc tgacgggatt gctggtaaaa
    10501 cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt cacagattga tggaaagcta
    10561 tccattcgca aacttgtttg gcatgaagga aagattgcgg tgtctcttgc tctgtataac
    10621 aaagcgaccc aattccaaag tttagctgta actgatacat tttattaaac tttgtctgga
    10681 ttttatcaag caatgatagc aatgaggctc tggaaatccg attgactagg ataatgattt
    10741 gttgtctatg tacagtgagc aaagcctgtc cgtaataaga gaagaccctt tcgagcaact
    10801 ccaataaagg attgatccgt tcttgacaca attgctgatt gtcagaaagt tgtcccacaa
    10861 ctacataata tggagaagaa aaatcaagct caattaagtg agggtggtat cgactatgta
    10921 cttgatatca tcaatgaacc gatggtactt tcttatgcga tggaacaggt agagcgattg
    10981 cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat taggtgagaa aataagaaga
    11041 taaaaataca cggtcccttc atggcgaccg tgcatttttg gtttttatta aatatttaat
    11101 actttattca agaaatcttt tgtccgttca ttttgcggat tttcaaagat ttgttgaggc
    11161 gttccatctt ctaaaaagtt tccgtcgtca atgaacatga cgcgattggc cacttcttta
    11221 gcgaacccca tttcgtgcgt aacgataacc atcgtcatac cttgttctgc taactttttc
    11281 atgacgttca agacatcacc gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa
    11341 agcatgatgt cagggttcat cgctagagca cgagcaatag ccacacgctg tttttgtcca
    11401 ccagaaagca tttcaggata catatctttt ttatcttcta gaccaactgt ctgcagtaat
    11461 ttgattgctt ttttctctgc ttcttctcta gattctttct tcaggtccat cggtgctaaa
    11521 atgatgtttt ccaaaataga aagatgaggg aataggttaa agtgttggaa taccattccg
    11581 atatgttgtc tcaccaagtt gatatctgtg cttttatcca tcaagtgggc gccgtcaata
    11641 ataatttcac cgtcagaagg ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt
    11701 cctgaacctg aagggccaat gatacagacg acatctcctt cgttgatgga tacattgatg
    11761 tcgtttaata ctgtgttatc gccgtatttt ttgactaaat gctcaactaa gattttttct
    11821 gccatcttac ttcaccttct tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa
    11881 aatcaagtac atgatcgcaa tgatcaagta aacatacgta ctttgtgttg tacgtgcaac
    11941 aatgatctta ccagtttgca gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc
    12001 ttttaatgag ataacgaact gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg
    12061 caaaatgatt ttttgcattg tccgattata agaaagacct aaacttctgg aagcttccat
    12121 ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc
    12181 gttaagagtc aaggtgataa caccagcaag gaagtcagga atcgtaaatc caacgatacc
    12241 aggcaaccca aagaaaataa agaacgcaag aaccatcatt ggaattcctc ggatgatatc
    12301 gacataaata ctagcaattg tgcgaagagc gcgtattggt gctacactga ataatccaaa
    12361 aatcacaccg attactatag ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca
    12421 aagtcctttc aacaatgctt gatagttgtt ttggataagt ccaagaaatg ttgattcatc
    12481 agcagtggaa gactcactgg aagtttctga accatccgct acatattctt taataatctt
    12541 atcgtattcg cctgttcttt ttaattcttt caatccttca ttgaacatat taagaagctc
    12601 aggattttgt ccttttttaa cggcaaatgc atattttccg ccagattctt ttttgatcgg
    12661 tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt accggataat catccatcat
    12721 tgcatcgatt gcgccaactt ttaacccgtc gtacaattga tcagcagcat caaaatattt
    12781 gatagaatag ccatattcag attcatggtc gcttaaaaat tcagcacttt cggtaccaac
    12841 ttttgctccg actgtttttc ctttcaaatc ttcataagac ttgatcgaag tatctccttt
    12901 tttgacagct aattggatcc cgctatcaaa gtaagaatca gaaaaatcat agcttttctt
    12961 tcgttcatct gttacagtca ttccggcaat cattccgtct gcttgtcctg attccaaagc
    13021 ttgcaaagca gcagagaaac cacggtagtc cattcggatg ttaaatcctt gcagtttggc
    13081 gatgttttcc atcaatttta catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc
    13141 aaatggagca aaagcagagt cactggcaat tacgtatacg tcttttttgg gctctatttt
    13201 ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg
    13261 accaaacgca agtgtcacaa gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt
    13321 tcttttcata atcaacctcc taaatttcca atattttatt attataggcg aaaagtgctg
    13381 aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg
    13441 ttataaaaca taacatgtat aataagataa aaaagtggcg aaagtatgtt cggtgtggta
    13501 taatttttta gtaaggaatg cctttgcttt ccttttcttt ttattggtcg taaaatgaaa
    13561 aggaatgaat gaaaaggagg acgaagaatg attgagagag atacctcccg acactttgag
    13621 cttgtctcga aataccaacc ggctggtgat cagcctgaag cgattaacca attagtggat
    13681 ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg cgactggaac agggaaaaca
    13741 tatacgattt ccaatcttat tgagaaagtg aataaaccaa cactgattat tgcccataac
    13801 aaaacattgg ctggtcagct gtacggggaa ttcaaggaat ttttccctaa caatgctgtt
    13861 gaatattttg tttcttatta tgattattat cagcctgaag cttatgtgcc ttcaagcgat
    13921 acgtatatcg aaaaagattc aagtgtaaat gatgagatcg acaaattacg acattcggct
    13981 actagttcat tattagaaag aaatgatgtg atcgtcattg catccgtttc atgtattttt
    14041 ggattaggct cgccttttga gtaccaaaaa caagtagttt cgattcgaca gggagcagaa
    14101 ctagatcgca atcagttgat tcgtgactta gtcagtatcc aattcgaaag aaatgatata
    14161 gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca
    14221 agagatgaac gtgccttacg tgtggagttt tttggagatg aggtcgaacg tatccgagaa
    14281 gtcaatgcac tgacaggtga agttttagga gaaacggaac acgtagcaat cttcccggcg
    14341 actcactttg tgacgaatga tgaacatatg gagcatgccg tagcgaatat caaagcggaa
    14401 ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta
    14461 gaacagcgaa ccaattacga tattgaaatg atgttagaaa tgggctacac gtcgggaatt
    14521 gaaaattatt ctcggcatat ggacggacgt aaagaaggtg aaccgcctta tacgctattg
    14581 gatttctttc ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa
    14641 atcagaggaa tgtataatgg tgaccgagcg agaaaacaga tgctagtaga ttacggtttc
    14701 cgtttgccat ctgctttaga caatcggcca cttcggttag aagagtttga aaaacatgtc
    14761 aatcagatca tttatgtctc tgcgacgcca ggaccatatg aacatgaaca aacagatacc
    14821 gttatccaac aaatcatccg tccgacaggg ctgcttgacc cagtgatcga agttcgtcca
    14881 atcatgggac aaatcgatga tctagtaggc gaaataaatg aacgagtcga aaaagaccag
    14941 cgggtgtttg taacgacatt gacgaaaaaa atggcagaag acctcacaga ttatttcaaa
    15001 gaattaggga tcaaagtcaa atatttgcat agtgatatca aaacgctgga acgaacagaa
    15061 atcattcgag atcttcgatt aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt
    15121 gaaggattag acgtacctga agtttccctt gttgctatct tggatgcgga taaagaaggc
    15181 tttttacgta gtgaacgttc cttagtccaa acgattggac gagctgctcg gaatgaagaa
    15241 ggtaaagtaa tcatgtacgc agacaaagta acagattcta tgcgtctagc gatggatgaa
    15301 acgtcaagac gtcgtacgat tcagcagaag tacaatgaag aacatgggat cgttccaaaa
    15361 acgatcatta aagaaatccg agatttgatt tctattacga aagaatcgga agatgataca
    15421 aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg aagaaaaaga cacgttattg
    15481 atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt tggattttga aacagccgcg
    15541 aatgtgcgag atatgatttt agaattaaaa gcttcaaagt aggaggacaa catggctaac
    15601 gataaaatta cgattcatgg tgcacgtgcc cataacttga aaaatataga tgtaacgatt
    15661 ccacgagata aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag ttccctagca
    15721 tttgatacgc tgtacgctga agggcagcgg agatacgtag agagcctttc tgcttatgct
    15781 cgtcagtttt taggacaaat ggataagccg gacgtagata gcatcgacgg attgagtccg
    15841 gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg
    15901 acggaaatca atgactattt acgcttgttg tttgcacgtg taggacatcc gatttgtccc
    15961 aacgatcata tcgagattac cagtcaatca gtagatcaaa tggcagatca agtaatggct
    16021 ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa
    16081 cataaaaaaa tattcgaacg aatccaaaaa gagggatacg tccgcgttcg agtcgatggt
    16141 gagatctatg atttaagtga agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg
    16201 atcgtcatcg accggatcat tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta
    16261 gaggcggccc tgcgattagc agacggctat gctctggttg acgtgatcgg tgaagaagaa
    16321 atgttgttta gcgaacacta tgcctgtcct tattgcggat ttactgttgg cgaactagaa
    16381 ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc cagactgtga tggtttaggt
    16441 gtcaaattag aagtcgatca agatttagtg attccagata gaacgaagac acttagtcaa
    16501 ggtgctatcg ttccatggaa tccaatcagc tctcagtatt atcctcaaat gttagcgcag
    16561 gcttgtgaaa gtttcggtat cgatttagat acaccattcg aagaattacc ggaagagcat
    16621 caggagatca tcttgaatgg gacagatgat cttttccatt tccattatga aaatgatttt
    16681 ggtggcgttc gcgatgtaga aacgactttc gaaggggtat tgaagaatat cgagcgtcgt
    16741 tatcatgata cgaacagtga tttcacgcgt gagcaaatgc gtctttatat gacagaatta
    16801 acttgtcaga catgtaaagg ttatcgattg aatccccaag ctctatctgt tcaaatcaat
    16861 ggaacgaata tcgggcaaac aaatgagttg tctattcaaa aagcagtaga tttcttctca
    16921 aacttaacgt tatctgaaca agagaaagta attgccaaac cgattttaaa agaagtaaat
    16981 gacagacttt cattcttgga aaatgtggga ctagactatc taactttgag tcgcgcttcc
    17041 ggaacattat ctgggggaga agcgcagcga atcagattgg ctacacaaat tggatcgaat
    17101 ctttctggag ttttgtatat tttagatgaa ccatcgatcg gattgcatca acgcgacaac
    17161 gatcgactga ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc
    17221 gaacatgatg aagatacgat gtgggcagca gattatttga ttgacgtcgg acctggggcc
    17281 ggagaacaag gcggacaaat cgtggctgca ggcactcctg aagaagtagc taatgatgaa
    17341 aattctttga cagggaaata tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc
    17401 cgtaaaggta atgggaaagc aatcaagatc actggtgctt cagaaaacaa tctgaaaaat
    17461 attagtgttg aatttccatt aggagaattt gtagcagtca caggtgtgtc tggttctgga
    17521 aagagtacat tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt aaataagaat
    17581 tctgctaagc caggtaaatt caagacaatt tccggctacg aaagtatcga aaagatcatc
    17641 gatatcgatc aaagcccaat cggccggacg ccgagaagta atccagcgac ttatacaagt
    17701 gtatttgatg atatccgtgg gttatttgct caaacgaacg aggcaaaaat gcggggttat
    17761 aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat
    17821 ggaattatta agatcgaaat gcactttttg cctgatgtct atgttccttg tgaagtatgt
    17881 catggtaaac gatataattc tgaaacatta gaagtgcatt acaaaggaaa aagcattgct
    17941 gatattttgg aaatgacagt agaggatgct gtagaattct tcaagcacat tccaaagatt
    18001 catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca
    18061 gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga attgcacaaa
    18121 atctctaatg gaaagaattt ctatatacta gatgaaccaa cgacaggact tcatagcgat
    18181 gacatcgccc gcttgttgca tgtattacaa agattagtag atgctggtaa cacagtttta
    18241 gtgattgaac acaatctaga tgtaatcaaa acagcagatt atatcattga tttaggacca
    18301 gaaggtggag aaggtggagg aacgatcctt gcgactggaa caccagaaga aatcattaac
    18361 gtaaaagaaa gttatacagg tcactatttg aaaaaaataa tggtataaag aggatagata
    18421 gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt aatatcagtg
    18481 ttctagaatc attacatggt agttctttct acactgattg gtatagataa aaacttccta
    18541 taagttcaat ccgttcaaaa aagaattgag caatcggagg actttccttt gattgctttt
    18601 ttctttatgg tatgatattc cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa
    18661 gtgagagata atgatgaaca gtattgaaaa tgaagaattt atccctatat tattaggaag
    18721 cgacatgaac gtttatggta tggcacgttc ttttaatgaa gcatacggca aaatctgcca
    18781 agcatatgct tcggatcagt tagcccctac ccgctacagt aaaattgtca atgtagaagt
    18841 tattccagga tttgataaag accctgtatt tatcgagacg atgcttcgtt tggcaaaaga
    18901 aagatattct gataagagta aaaaatatct tttgattgct tgtggtgatg gatatgcaga
    18961 attgatctcc caacataaac aagagctgtc agaatatttt atctgtccat atattgatta
    19021 ttctttattt gaaaggctga tcaataaagt aagcttttat gaagtttgtg aagaatatga
    19081 tcttccgtat cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg gacatttgga
    19141 acaggaattg ccttttgaat tcccagttgc tttgaaacct gcaaacagtg tggagtatct
    19201 ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta gagactagag aagagttcga
    19261 tttaatttta ggccggatat acgaagccgg ttataaaagt gaaatgattg tacaagattt
    19321 tatcccgggt gatgatagta atatgcgtgt gctaaatgcg tatgtagatg aagatcatca
    19381 agttcggatg atgtgccttg gtcatccact attagaagat cctacaccag catcgattgg
    19441 taattacgtt gtgatcatgc cagattataa cgaaaaaatc tatcaaacca ttaaggcatt
    19501 tttggaaaaa atcgaatata caggctttgc caatttcgat atgaagtatg atcctagaga
    19561 tggagaatat aaattatttg aaatcaattt gagacaaggt cgtagcagtt tctttgtgac
    19621 attgaacggc ttgaatctgg ctcgttttgt cacagaagat cgagtgttca acaaaccctt
    19681 tgttgaaacg acctatggaa cgaatcaatc agataaagca agattatgga tgggtgtgcc
    19741 caaaaaaatc tttttagaat atgctcggga aaatgaagac aaaaaactag ctgagcaaat
    19801 gatcaaagaa aatcgttacg gtactactgt attttatgaa aaagaccgat caatcaagcg
    19861 ctggttgttg atgaaatata tgttccataa ctatattcca agattcaaaa agtattttca
    19921 tgtgaaagaa ggctagttca tggagaattt tttcagtatt ttaggcggaa tgggcacgat
    19981 ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga
    20041 atatttgaat tatgtcttat tcaatcatgc aacagttcct gatcgtacag cttatatttt
    20101 agatcgatca gaagaaaatc caatgccgtt tctgctggat gatattgaga aacaaaattt
    20161 attgcgacca aacttcattg tgttgacttg taacaccgca cactactttt ttgaagagtt
    20221 acaagcggca acggatattc ctatcttgca tatgccaaga gaagcagcaa atgaacttgt
    20281 ccgtcagcat acgacaggga gagtagcgat tctaggaaca gaaggaagta tgaaagctgg
    20341 aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca gtgattcctg atactgcgct
    20401 tcaagaaaaa atcaattacc tgatctatca tgagatcaag gaatcggatt atttgaatca
    20461 agagctctat tacgaaatct tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat
    20521 cctaggttgc acagaattat ctttgatgca tgaatttgct gaagataatc attacccagt
    20581 gatcgatgct caatctattt tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag
    20641 cgaggcattg gatacagcat cggaaaagta aaaaaattgg atttggggca aatatcctaa
    20701 aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg
    20761 gatgatataa ttttattatg cacaatgtga gaggggttcg taaatatgac agataatttg
    20821 caattagtca tcataacagg tatgagtggt gcagggaaaa cagtagctat ccaaagtttt
    20881 gaagatatgg gctatttttg tatcgataat atgccgcctc gattgattcc aaaattctgg
    20941 gaattgatca aagaatcagg taaagtcaca aaaatggctt taggagtgga tttgcgttct
    21001 cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc
    21061 gacacttcta tcttattttt agatgcttca gacgaagaac tagtggctcg ctacaaagaa
    21121 acaagacgag cacatccaat ggcgatggac ggtttgatca ctgaagggat acgtaaagaa
    21181 cgggcgatac tagacgactt gaaagcccaa gcttccgtga tcattgatac gacaacattg
    21241 acaccacgac aattacgtga aaaaatcaat gagtcattca aatcaaaggg agaccaagga
    21301 tttcggatcg aatttgtatc atttggtttt aaatacgggt tgccgatcga ttcggatatt
    21361 gtgatggatg tgcgtttttt gcccaaccct cattatatcc cggaactaag accattgacc
    21421 ggaaaagatc agccagtcta tgattatgtg atgtcctttg atgaaacgca atcattctac
    21481 cataaatttt tggatcttct ggaaactgta ttgcctggat acattaaaga aggaaaaagc
    21541 agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc attgactgaa
    21601 cgtttggcaa atgctttagg gaaaaactat aaagtcaatg tgacacatcg ggacaaggat
    21661 aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa
    21721 agatcgtagt ggtcggcggt ggcactggtc tgccagttat cctgaaaagt ttacgaaatc
    21781 aaggagcaga tatcacagca gtagtgactg ttgcagatga tggtggaagc agcggtgcaa
    21841 tcagagaatc gatcgctatg gctcctcctg gtgacttgag aaatgtctta gtggcgttat
    21901 cggatatgcc acaattctat gaagatattt tccaataccg tttcaaaaag gaagatcagt
    21961 ttttagccaa tcatacgatc ggtaatctga tcattgccgc agtttccgag atgcggggaa
    22021 gcacttatga ggcgattcaa ttattagcaa aaatgatgca tgtagatgga catgtttatc
    22081 cttcttcaga aactcctttg accttgcatg ctgtttttaa agacggaacg actgcagtag
    22141 gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa
    22201 atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg gacgccgaca
    22261 tggtcgtttt gggacccgga agtttattca ccagtatcct tccaaattta gtgataccag
    22321 aaatcggcga agcaatgctc aagacaactg ctgaaaccgt ctatatctgt aatatcatga
    22381 cccaaaaagg ggaaacggaa catttcacag atgccgatca tatacgtgtt ttacacaagc
    22441 atttgaatca gccttttatc gatacagtat tggtcaatac ggaaaaagtg cctgaagatt
    22501 atatggaccc agagatctat gatgagtatc tagtacaagt cgcacacgac ttcaacggat
    22561 tgagaaatga aggatgtcga gtgatttcca ccgacttttt agaattgaaa gatggtggtg
    22621 tcttccacga tggagacaaa gtagtagaag aattgttccg tttagttttc ggttcaaaat
    22681 attaagcaat ttattgttgg agaaaggagg agaataggat gtcatttgct gcagatgtga
    22741 agaaagaact aactggtttg gaagttcatc gagaacatgc gaaagcggaa ttagctgctt
    22801 tgctgcgaat gaacggctct ttaggtattg tgaaccagca gtttattttg aacgtccaaa
    22861 cagaaaatgc tgcaattgcc agacgtatct attctctttt gaaagatcat tatcaggtac
    22921 gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc
    22981 ggttgaagca aggcacaaaa gatgtgttga tggatcttga tatcatggat ggtatgatgt
    23041 tcaatggaca cgtttcggac gagattatgg gaaattcgca aaaaatgcgt tcttacttac
    23101 gtggtgcttt tatggcctca ggttcagtca ataatccgga aacgagtcgt tatcatttgg
    23161 aaatattttc tatttatgaa cagcataacc aagacatttg cgatatgtta aattattacg
    23221 gtctacatgc tcgaacgctt gaacggcgca atggctatat ttcgtattta aaaggagcag
    23281 aaaaaattgc tgatttctta acattgattg gtgcaaccaa ctcaatgctc cgatttgaag
    23341 atgttcgaat cgtacgggat atgagaaatt cggtcaatcg tctagtcaat tgtgagacag
    23401 caaatctgaa caagacgatt gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg
    23461 aagcaaaggt cggtctacat gcccttcctg aaaaacttca ggaaatcgca gaacttcggc
    23521 ttcaacatcc agaggtcagc ttgaaagaat taggggaaat gattccctca ggtgcaatat
    23581 caaaatcggg aattaatcac cgaattcgca aaataaatga atttgcggaa aaactgcgtg
    23641 aagaagcagt ttaatccaaa gaaaaaactg atacatgaag caggagatgc gaatgtatca
    23701 gtttttttta aaagctatac ttgcttttct tccgtcaaac tataattcca actttactgg
    23761 agtaacataa tgttgaaagg cttctattgt tttttaatac aatcgtataa aaagcgggga
    23821 gacaactgaa tgaagatttt agttttatac ataaactttt gcttaatata gaagtcctga
    23881 tatagatagg atcaagtatt tatctataac tggagattaa ttaaaaactg gaaggaactg
    23941 ctcgatcaac cttgagaact gtttttctta cttctggtca agattctcgt ggttggtgga
    24001 taaggatggt tgataagttc attggattag ctagtcgaaa acaaccaggt gtaattaatg
    24061 tagaagtttt aacaagggga tggagtgatt tattcagtga taatcataag ttcataaata
    24121 gtaattggga tgtatggttg gtatacgtta tacctgaact tgaactatca agatttggtt
    24181 tagaatttga gaatcgatgt ttataaaggg agggcaaaaa atgtgctttt ttacaacttt
    24241 tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc gaaatacagc
    24301 atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat cgatcgttgg
    24361 attttttcta ttcacagtta ttgtatttat ttggagaact acagcaccac cattgccaga
    24421 gattacatat ggagaatttc cgtttagtct tgaatatgaa atagatgagg aaagaatttt
    24481 gattgaagat acggtgattg cagaatttat aggaagtttt agagggaatg tgacaactca
    24541 acctagacga agttggagta taaaactttt aaataatagt ggagagttga aagcatttca
    24601 ttatgaattc ttaataatag aaagtgaaga tgtaacaatc actttaagta gtggctctgc
    24661 tggatatttt atgaatgatg gtggaagcaa gccacagctc acagtccgtg atcgtattgc
    24721 aggttttatg catttttctc actatgactt agaagaagct cgtaaaatac ttgcagaata
    24781 tgggatcaca ttaatcaatt gggaatatgc gccaccgata agaaattttt tcaatgatta
    24841 ttattagtct tataaaaaac agaatgtaaa atttatgtgc gcgagtattt agttttccat
    24901 tttttgtttg atagatatat agcattgttg aagcatggga tagtggtgcc aattttatca
    24961 atgaggttgg tcaggcagtt aacataattg gtgatgctat aaatccattt aattggaatt
    25021 ggaggaatta ggtgtggaaa cagtgagatt atggatattc atgaaaaatt ttcgttcggc
    25081 tttgagatat gttattctgg aatcaaaatc aggttattat ctgattgaca cagatcacta
    25141 tactttttgg ggaggctacc tttttccgat ttttaattgg ataatccccc ataaggttat
    25201 gaaaattaac ttgcctgcag aagaaataaa tagattaata ctcaacaaag atttacaaag
    25261 gaaaagagag aaagaacatt ctaatgttgc ttggataggt ccagcatttg cagtagtgac
    25321 ttcttcaatt gtcggtccta tgctagagag tatttatagt gatttttcta ttggaatacg
    25381 ttatttattt gttactttaa gtatagctgt tcttttgtgc ataaagggtt atgttagcaa
    25441 gaagaataaa gatattttaa cactaatcgg tgtaaacaat ttatctagta ctaaaattat
    25501 actgtttcct gaatctataa aagaaggatc aaggttgttg ataattttta taatgcttat
    25561 actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DACCZY010000030        24912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCZY010000030 DACCZY010000000
VERSION     DACCZY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.030"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(69..680)
                     /locus_tag="IVZ67_001019"
     CDS             complement(69..680)
                     /locus_tag="IVZ67_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8165108.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(803..928)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1010..1726)
                     /locus_tag="IVZ67_001020"
     CDS             complement(1010..1726)
                     /locus_tag="IVZ67_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8165109.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1732..2301)
                     /gene="scpB"
                     /locus_tag="IVZ67_001021"
     CDS             complement(1732..2301)
                     /gene="scpB"
                     /locus_tag="IVZ67_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8165110.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2285..3082)
                     /locus_tag="IVZ67_001022"
     CDS             complement(2285..3082)
                     /locus_tag="IVZ67_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8165111.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3052..3423)
                     /locus_tag="IVZ67_001023"
     CDS             complement(3052..3423)
                     /locus_tag="IVZ67_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8165112.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3442..4329)
                     /gene="xerD"
                     /locus_tag="IVZ67_001024"
     CDS             complement(3442..4329)
                     /gene="xerD"
                     /locus_tag="IVZ67_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8165113.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4425..4898)
                     /locus_tag="IVZ67_001025"
     CDS             complement(4425..4898)
                     /locus_tag="IVZ67_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8165114.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5003..5866)
                     /locus_tag="IVZ67_001026"
     CDS             complement(5003..5866)
                     /locus_tag="IVZ67_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8165115.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6062..8161)
                     /locus_tag="IVZ67_001027"
     CDS             complement(6062..8161)
                     /locus_tag="IVZ67_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8165116.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8381..9490)
                     /gene="rpoD"
                     /locus_tag="IVZ67_001028"
     CDS             complement(8381..9490)
                     /gene="rpoD"
                     /locus_tag="IVZ67_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8165117.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9514..11355)
                     /locus_tag="IVZ67_001029"
     CDS             complement(9514..11355)
                     /locus_tag="IVZ67_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8165118.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11537..13930)
                     /locus_tag="IVZ67_001030"
     CDS             complement(11537..13930)
                     /locus_tag="IVZ67_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8165119.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14037..14897)
                     /locus_tag="IVZ67_001031"
     CDS             complement(14037..14897)
                     /locus_tag="IVZ67_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8165120.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14956..15477)
                     /locus_tag="IVZ67_001032"
     CDS             complement(14956..15477)
                     /locus_tag="IVZ67_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8165121.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15797..16516
                     /locus_tag="IVZ67_001033"
     CDS             15797..16516
                     /locus_tag="IVZ67_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8165122.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            16542..19133
                     /gene="mprF"
                     /locus_tag="IVZ67_001034"
     CDS             16542..19133
                     /gene="mprF"
                     /locus_tag="IVZ67_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8165123.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19321..20508)
                     /gene="tuf"
                     /locus_tag="IVZ67_001035"
     CDS             complement(19321..20508)
                     /gene="tuf"
                     /locus_tag="IVZ67_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8165124.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20876..21232
                     /locus_tag="IVZ67_001036"
     CDS             20876..21232
                     /locus_tag="IVZ67_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8165125.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21473..22477)
                     /locus_tag="IVZ67_001037"
     CDS             complement(21473..22477)
                     /locus_tag="IVZ67_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8165126.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22643..23494
                     /locus_tag="IVZ67_001038"
     CDS             22643..23494
                     /locus_tag="IVZ67_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8165127.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23511..23756
                     /locus_tag="IVZ67_001039"
     CDS             23511..23756
                     /locus_tag="IVZ67_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165128.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23943..24647
                     /locus_tag="IVZ67_001040"
     CDS             23943..24647
                     /locus_tag="IVZ67_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165129.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7003 a         5167 c         4354 g         8388 t
ORIGIN      
        1 acattattgc atgtcttgta tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt
       61 tttgatattt atttgactgc tgtatgacgt tgttcgaact gatgttgttt tcgacttaac
      121 catggaagaa gttgcgcatg cagcactaaa aagacagcac tcacgatgaa tcctttgatc
      181 aagttgaaag gaacgatccc aatcagtaca tagtttgcta aagacatgcc cagcatctgt
      241 gttgcgttca gccctaaaaa cattaagtaa agaggcgtaa taacgaaata atttgcgata
      301 ctcataaaaa tagttagagc gatggttcct gtaatgacac ctaaaaattt gtttcttcct
      361 ttatgctgtt tctttacact aaagaaataa taaatcggga atgtaaagat cgttgttgat
      421 aagaaactag cagtgtctcc taccaaatta tccggagaaa aacctgtagt cagcagatgt
      481 aatactgaac gcaagaacgc tgttgcaact ccggccgctg gaccgaataa gaacatgctg
      541 atcaataccg agatatcact aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga
      601 aaagcaaaat actgtaaaac taatgcgatc gcagcaaaca tcgctactgc aaccattttc
      661 tgtacacggg tgttcttcat ttgaaatcct cctgttagga ctcctcttca gcgaactttt
      721 tgacgtttct ctccaaatga aaaaactcta ttttccaggg aaaatagagt tagagttatt
      781 tgatcaagcg tcgtcaaata agctctatct tctttatcca gactatactg tcggtatcgg
      841 aatttcaccg attcagctat ttttttgcaa aataggtcgt ggactataac caccggtcgg
      901 gaatttcacc ctgcccctga agacgaacct tatataattt ttgtttacat attcattata
      961 catatctatt caaaaaacgc aacatctaac acttataaaa agtaagtgtt cattttgagt
     1021 ggttcagcaa actgctgact tcttttttgt tcagttcacg gtaatcccca ggacgtaggc
     1081 cttgtaaagt cagatctccg tatttttcac gttttagttt ttgcacagga agaccaactg
     1141 cttgaagcat atttttcact tgatgattac gtccttcgtg gatcgtcaat tccacgatac
     1201 tgtggtttgt cttaggatcc actgataaaa tttggaattt agctggagat gtttttcgtc
     1261 cttcgatctt tattcctttt gtcaaaggtg caagcatcga ttttgtagcg atccctttta
     1321 cctttgcaac gtaaacttta tccacttcat gttttggatg tgtcaggcgc tgagcaaaat
     1381 ctccatcatt tgtcagtagc aagatccccg aagtatcgta atccagtcga cctacaggat
     1441 agatacgttc ttttaccatc ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg
     1501 caacagcaga aataactcct ttaggtttgt acaataaata gtaaccgtat tcttcttgat
     1561 aaattgggac ttcatccact tccacagtgt catgtttccc aacttttatc cctaattccc
     1621 ttacaacttg tccgtttact tttacacgtc ctgcaaggat gaattcttct gcttttcgac
     1681 gagaagtgat gccagcgtgg gcaattactt tctgcaatct ctccattaca attattctcc
     1741 tttattcatt ccatcaaaga atagatctgg tgacacgtct tcttcaagtg aaatttccat
     1801 ttcttgtgta tcaggcagtt cttctaatga tttcaaacca aaataatcta agaagtagcc
     1861 agttgttcca tataatatcg gccgacctgg tccttctact ctaccttttt cttcaatcag
     1921 tttatgggca gctagacgct gtaccgctcc tgaaacctga actcctcgaa tctgctcgat
     1981 ttcagctctt gtgattggct gtttataagc gatgattgct aaggtttcga cggcggcttg
     2041 agataatgtg tttgatgtcc cttgcgcata tcgcttaagt aagggagcca gctctttttt
     2101 agtcgttaat acataatggc tttccgtttc aaaaatatgc aaagctgagt gcgagttttg
     2161 agtgtaagat tcttttaatt ctttcactaa acttgtgact tctgcttcgc tgagatttaa
     2221 cagtccgctt aattcttcat tactgatccc ttcctcgcca acaataaaaa gaagggcttc
     2281 aagttcactt aatttcgtca tgttcggtat ctcctactgc tggataaagc ataattgatt
     2341 catagttttc tttttgttgc gcaatgatct gccctttttt catcaattct agcaaagcca
     2401 taaaagtagt gacaacttca cttttagaag gtgactcaaa aaaagaatcg aaagtacagc
     2461 catgcgcatt tctttttata cgctcttcga taaataccat tcgttcttcg atcgttgtgt
     2521 catcgggcgt gatcgttttt tccacttttt gagaaaattt cttacgttct aagattttct
     2581 gcatggcacg gatcaaatcg atttttttga cttgtcctcg cttcaacggc ttttcagcat
     2641 ccgctaaccc atcgatgttc ataggttctt ttgtgtaata aagacttcgt tcctctgctt
     2701 tttctgtcag tctttcagct gcatatttat atttacggta ttctaaaagc tgagtgacca
     2761 attcttctcg aggatcttct tcccataatt cttcatcgtc tgtgacgaat tcttggacag
     2821 gaagcaatgt cttgcttttg attgccatca atgtagcagc catcactaag tattctcctg
     2881 caacagctag ctcgagtgtt ttcattgtat ggatataatt catatattgc tccgtgatcg
     2941 tagcgatcgg tatatcatat atatcgattt ccatttgctg aatcagatga agcagcagat
     3001 cgagaggacc ctcgaataca tctaatttaa tattgatctc cgtcaactca ttcactcttt
     3061 cttctttttc tttgctagcc attttccaat gtaaggctct gtgatttcag ttgcacggat
     3121 ggagacatcc gggtactttt cctgtaattc atctaacatt tgaaagccca tattctcacc
     3181 gcgataagac ggattcaaag aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta
     3241 aataccaata atcccttgaa tgttggttga accttctggc gtaaagaaaa aaagttcata
     3301 atcgtcttca gattcgtacg tatcaatctc cttcaacaaa gaagttttac ctgtcagttt
     3361 cttatggaaa ctcaatagtc ccatagcaat tttacggttt tctttccggt aatgtgttaa
     3421 catcttctca ccttcctatg actaagccct tggaaaatgc tgcttataaa catccgtcat
     3481 tcttttcttc gtgatatgtg tataaatctg agtcgtagag atatccgcgt gacctaacaa
     3541 ttcctgtact gtccgcaaat cagctccatt ttctagcaga tgtgtggcaa agctatgccg
     3601 cagcgtatgt ggtgttactt ctttgtaaat cccagcctct cgtactaact gcttcagatt
     3661 tttccagatt ccttgccgag aaagaccttt cccatgatta ttgacaaata catgcatttc
     3721 agatgcgtcc tttacaagta atggacgtgc ttcgtctaag tagcgttcca gccattggat
     3781 cgcataatca cctaaaggaa tgatccgctc tttatctcct tttccaactg tctgtaccag
     3841 tccaagagaa agatgaagat ctcccaattt caatccaatc aattcactga cgcgcatccc
     3901 agtagcatac atgacttcca agattgcacg atctcttatc cctaacgttt ttgtcgtatc
     3961 aggtgcttca atcagctttt ctacttctgt cagagtcaat gtacttggta atttctgtgc
     4021 tttcttcggc gtatcgatat gttgcatcgg atcatgatca gtgatacgct cttgccgtaa
     4081 aaactgatgg aatcttctga gacttgagat catccgagta acagttgctg tcgctttttt
     4141 ttcttcatgc aacttctcca gaaaacggat gactaaaaag cgatcgacat cctgccatga
     4201 agcaattttc tgctcctgta gaaaatgcaa gtactgttcc aaatcacgtt catagctttg
     4261 tcgtgtattg gcggacaatc cccgttcaat attcaagtag tggagataat cgttgatttg
     4321 ctctttcata ataatccccc tcatgcctct ctatcatatc agataccgct tcattctgat
     4381 agtctttttc cataatctga gcaaagaaaa agccgtctag acactcagac ggactggttt
     4441 cttttttctt tttcatagca ttcctgacag attccatgga acgttagccg atgatctttt
     4501 actaaaaaat gataacgttc ttctactact tgctccactt caagtaaaag atcctcttct
     4561 acttcctcga tcgtgccgca gttcaagcat agtaaatgat gatggaaatg tttggcgcct
     4621 tcttttctca aatcataacg ggctaatcca tcattaaaac ttactttatc gacaattttc
     4681 aattctgtca agatttcaag ggttcgataa acagtagcaa gtccaatttc cggactcttt
     4741 tttttcacta aaaagaagat ttcttctgca gataaatgct ctttctcatt ttccaaaagc
     4801 acaagcagag tagcttctct ttgcggtgtc agcttaaacc ccgcttcatg tagctgctgt
     4861 tttgtttttt tcatcgccga aatagcattc atgaccattt actcaacccc aacaatttat
     4921 aataattaca aataaaaaaa gatataacac tatgttaatt aaaattatta taagctagtt
     4981 aagaaaaaca tgcaagggat tctcaattag attttttgac tagttttgtc acaccgtttt
     5041 cttgtgtaat caatccttgt ttcaaaagat tacctaatgc gcgcttaaat gctcctttgc
     5101 tgataccaaa agctttcatg atttcttctg gatccgattt atctgtaaag ttgatttgct
     5161 gatctgcaga acgttctaaa atggttaaaa tcatttgtgc atcatctgat atagcttcat
     5221 atgctcttgg cttcaatgaa agattcagta caccgtctgg tcggacacca atcactcgac
     5281 cttttatctg ctcgcctaga cgaggttcat tatatcgttc agaaggatgg ataaacccaa
     5341 gatagaaatc ttccgttaaa acgtacgtac ctgttaattt caagcggtat gctgttccta
     5401 tcacatcttt gtttttcatt tcttctgttc catatttgct taaagatttg aatatttttt
     5461 catctgctaa actgccccaa atacggtctt ttgcatcaac tctaagggaa atcatcaagc
     5521 gatccccttt tttaggccat agctctgtca ttgtcggcaa ttcatcaaga gatacgacaa
     5581 agtctttatc tggaagaccg atatcgacaa acgcacctaa atcttttcgt gtagcagtaa
     5641 cgattccaaa agcgtaatga ccgatacggc ttttaggaat ttgtgtagtg atccgatttt
     5701 catgtttttg attttggtaa ccgaatcctt caactgcttc accgattttg tgttcacctt
     5761 ctgatttgct taagccaaag gtttgtccgt ttttttggac aaagtagttt ttttcatttt
     5821 catcaataat aatacctgta aatatagttg ctaataaatc gttcattttt ttctccctct
     5881 atagtgaaag gttttcttaa tacatttctt tgctatcata acataatgtt atctacttga
     5941 atagctgagc cttggtttgg aaaggtgaaa aaaggagtat gacaacagtt gtgtcatact
     6001 cccctttttc gaataaacgg tgttaaacgg caggctcaca gcctctttca tttgcctaaa
     6061 attaattgct cccatttcct ccgaaccatc cagttttaca gttgatggtt acgatgtaaa
     6121 atggcaaagc actttctcca atgcctttca tcaatgcaga ttttgttgaa gtggcatata
     6181 gattataata gccttcccca ttttcgattc tggctttttc taatctgtaa cctccttcaa
     6241 cgatatatcc acgttggata catgtagcaa tcactttgct ttggacacct ggtgcccacg
     6301 tccatgctgg attagatgca tcggcaatat cacttgctct ggcagatttg attggctgat
     6361 tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct gcttgtgcga
     6421 ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg agcggcttgt tttgcttgtt
     6481 cttcagctgc ttttttcgct tcttctgctt ttttcttttc ctcttcttct tttttctctt
     6541 tatcagtgag catcttatct acttgtttca attgttcatt caactgagca gatagatctg
     6601 tatttttcac tttttctact gcttcttttg ccgtcttgta ttggtcgcgt gttgctgaat
     6661 ccactacttt tccgtcttta aagactatag ctgttttttc tttagctgtt tgcaattcag
     6721 catattgatt tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa tctgtgttat
     6781 ccgtttcgat tgcttggatc tctttatctg ctttcaactt gatgtcatct gctagatgat
     6841 catcgacgat ggcaggtgct atgaaccact cattcacttt ttggatatct cggatcttag
     6901 attggatatc ttggtaagtt gtttctaact ctgaatatcc tttttcattc ttaagactgg
     6961 ataaatcatt ttttagtttt gataaatctt gatgaatcat atctgcacga ataaattgat
     7021 ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag ttcttccatt tgttgctgtc
     7081 tttttgcttc tgctgctact tcggcttcta ctttttgatg atggtcataa taagcccagc
     7141 ttcctccacc gagtagaagt actgctgcta aaacgagata aggtactttt tttgattttt
     7201 tcttttgcgc tttatctgga tctgtttttt ctaagtttga ttcttgatgt gttttttcag
     7261 tatctggcac gattgttatt gatttcgctt ctgcttcaaa cagcttaggc tggttctctt
     7321 ctttttctat ttttgcttca agatcagtac cctctttgct tacagattga ttttcagcat
     7381 cggattcact atttatttca tcgggctctg ttgacttttt gtctgtgttt tcgagtattt
     7441 cttttttctc tgcttccggc agtttattcg ttttttcctt ttctaattgt ttttcagtaa
     7501 atacttcagt tttttctgtg atatccgttt ttacgtcttt tatttttact gtttctttac
     7561 ctgtggcgtt ttctctatca gagtcttttt cagtttcttg ttccttttct ggctctactt
     7621 tgttttcctg tgcaattttt tcactgatca ttttgagcag ctctttcgcg tgctcttctt
     7681 ttgtatcttc acgatgtgct ttgatataag cggatagtat cgaattttct tcctcattat
     7741 cgacgatttc tttcagttca gctactcttc ttttttgttt tgattcaagc gtttcttctt
     7801 gattctttcc tgattcttcc gaagtctcat gaacagttgc tttcttctgt tcatttttct
     7861 ctatattagt tacttgatcg tgagcaggtg gttcttcgtc ttctggttct gctttttcga
     7921 ataattccat tacagattct agaggtaaat ctttcaattc cgaccattcg attggatcgt
     7981 ttaattctgt atcaggaaag actgcatctt cagttgattt atcctgcata gctgcatctg
     8041 tatttgtttc ttcaattgat tcttttattt gctgatcttc tgcagttatt tgatgaccgc
     8101 agtctggaca gatagtattc tgctccattt ttttcgatcc gcaatttgga cattttttca
     8161 tcacttagcg atcggctcct ttactaggtt tacgctacca tttaatctat catattctat
     8221 acgtcacgcc aatagcttat ttttaggatt tacttaaaaa taatgaatga atttccttcc
     8281 tatagaaaaa aaagcttgag actgcgtagt ctcaagcttt ttttctttta aaataaacca
     8341 aacagtttta ctgatttctc ttctggtcaa tagtccacgc ttattctaag aaatctttta
     8401 attgttttga acgagaagga tggcgcaatt ttcttaatgc tttagcctcg atctgacgaa
     8461 tacgttcccg agtaacacca aatactttcc ctacttcttc caatgttctc gtccggccat
     8521 catctaaacc aaaacgtaag cgaaggacat tctcttcccg atcagtcaat gtatctagta
     8581 catcttccaa ttgttctttt agtaactcat aagctgcgtg ttctgctgga cttgttgctt
     8641 cctggtcttc tatgaaatca cctaagtgag agtcatcttc ttcaccgatt ggcgtttcaa
     8701 gggaaacagg ttcttgcgcg attttcaaga tttctcggac tttttctgtt ggaagatcca
     8761 tttccgcacc gatttcttct ggcgtaggtt ctcttccaag gtcttgcagc aattgacgtt
     8821 ggatacgtat caatttatta attgtttcaa ccatatgaac tggaatacgg atcgtacgtg
     8881 cttggtcagc gattgctcga gtgatggctt gacgaatcca ccatgtagca taggttgaaa
     8941 acttaaatcc tttgcggtag tcaaattttt caactgcctt catcagtccc atgtttcctt
     9001 cttggatcaa gtcaaggaac tgcatgcctc gtcctacata acgtttagcg atacttacta
     9061 ctaaacgaag gtttgcttct gccagacgct gtttcgcttc ttggtcgcct tcttcgattt
     9121 tcaatgctaa cgctacttct tcttccgcag ttaaaaggga gacacggcca atttctttca
     9181 gatacatacg aaccgggtca ttgattttta caccggttgg cgcagaaaga tcttctgttt
     9241 ttgcttgttc agcttttttc tcagcactct tcaagctgtg aattgatgga tcaccatttt
     9301 catccaccac actgatacct gcgtcttcaa tcttttggat caatttgtcc atttcatctg
     9361 cattcaaagt gaatggtgta gcaagctgat tggataaatc atcatataca acttgtcgtt
     9421 tgggtttgtt ttctttgatg aaagctgcta ctgctgcttc atacgttttt ttatctgttc
     9481 catttgccat aaaagaaggc cccccttgag tagctatttg gcttgtttta actgtttcgt
     9541 taaattgatg atttcaatcg tcaattcaag ctcaagctgt ttgtttccag ttcgtctggc
     9601 ttcttgctgc atttgttttt ttaatgttat ttcatcagct aagccagatt tgctgatcac
     9661 atctagcaag tcttggattt ctcgtggcga actttcttct gccaccggta aatattcgat
     9721 ttccaccacc aaccgcctta aattttcatc ttgcaaaaag tctaaaaact ctgcaaggtt
     9781 gaattcataa tgttgtgaga catatgcatc gaataaaaaa taaacttctg catatgcatc
     9841 atgaataaac tgaaattctc gttcttttaa aatattacgg acagaaactt ctttaaatgc
     9901 acgatataat agaatctgct ctgccttttc tacttgcgtc aaaggtctct ttttccgtgc
     9961 agtttcttgt ctcgtaacct gagtgtgttg actttcctga ggttcttgac gattttgctg
    10021 atttgtcctt cgaagatcgc gcagttgttt ttgtatcgtc tctctgccga tctgaaactc
    10081 tgttgacagt tgagatagat acatatcttg ttcgattggt gaaacaactc ggctaagttc
    10141 aactagtaat tcttcgaggt attcgatctt gtctttttca ttatctaaat ttttcccttg
    10201 ttttaaataa cgtgatttaa aagtaaagac tgtttcgcgc ccatgagcca ctaattcttg
    10261 gaaagatgct tctccatact tacggagata ttcatcagga tcaagctttt ctggaacgac
    10321 tacgacagaa agtgctaatc gactgttttg ccgaagcaat tcaatccctc gattggttgc
    10381 ttcaaaacct gcgttgtctc cgtcatagca aaagaccaat tctttcgtca gcttttccat
    10441 ttgttggatc tgttgagctg ttagacttgt ccccatagaa gctaatccag ttttgatacc
    10501 agcctgccat gcagcaatca catccataaa cccttcgaac aacaaaattt ctccgttttt
    10561 ccggatatcg cttcttgctt tatcaagatt aaataaaact tgtcttttat tgaacagttc
    10621 tgtttctgga ctattcaagt atttgggctg atctttttcc tgcccttttt caggtgcaag
    10681 ccagcgtcct gaaaaaccga tcgtctggcc ttgagaatta cgaagtggaa acatcactct
    10741 ttgataaaaa cgatcagaca tacggccatc atcatgcgtg acaaacaatc cagagcgttc
    10801 taacaattca tttgaaattg attcattctt gaatacttgc tgaagaaaag aacgctcatt
    10861 aggtgcaaaa cctatgttga attcttcaat taattctaaa gacaacccac gactttgcaa
    10921 atattctaaa gctggctgtc cagcttttgt atgcaatagc atgtgatgat agatttccgc
    10981 tgctttttca tgagcagcaa tcagtttccc cgtagcagat tcaggatttt ggacagctat
    11041 agtacgctga cgccattgat cttctaacgg tatctgttcg aagtctgcca cttttaccac
    11101 tgcttctgga aaacttagac cttctagttc ttgaagaaaa gtaaacacgt ttccgccttt
    11161 accacaacca aagcaatgaa aaatctgctt gtcttctgca acagaaaaag acggtgtacg
    11221 ttcttcatga aaaggacaca gccccaagta atttttacca gactttttca actggacata
    11281 ttgtccaatc acctcaacga tattcgtttg actgcgtact gtttcgataa cctcttgagg
    11341 aatccgttga gccatttttt caccccctgc tctattgata tcttttgggt ttaacagaag
    11401 atatttcagg agaataaccg gtaaaaaatc gcactaactg aaatcagtgc gattttaaag
    11461 gacacaatta tacatgtttg attgtatcat atttcattgc tttttcaagt ataacacatt
    11521 tctatctcat taagcatcaa tttgctactc ttttcagtaa ctgattttga atatcagcct
    11581 tccagtacaa tactcgaaga acaatattta cgagcatgaa tacaaagaag tatcctacgc
    11641 tctctcccaa ccctaagttt acttccaaaa ttccatggaa cagcattgca gctacatata
    11701 tccctacgat cgtcgttact gctaggaaga tgcgcaatgg gttgaaggga taacaagctt
    11761 ttacaactgc catgcaactg atcccaatca gcaagtaata catcaaagta gtagtttcag
    11821 catttcccca tgcgaatgtt tttccaagta gatacaccgc aatgatattc actactacaa
    11881 gcaaggcatt tggtaaagca ttaataagtg ctgtaggcaa ataacggtaa gtgatttttc
    11941 ttttatcttg ttcaaaagat aaaaagaagg aaggataccc ttcaatcgct aaatcaatca
    12001 gcgtgatttg gatagggata aacggaaatc caattgctgt aacggcacat ataatcgata
    12061 aaataaatga ataaatcgtt ttgataaaaa agatgcctga gactttcgtc acattgttga
    12121 ccactcggcg tccttcaaac aggacttctg gcaatgtagt aaaatcagag tccaacaata
    12181 ccagattcgc aatctgacgt gttgctccat caccttctgc catagcaatg ctgcaatctg
    12241 cttctcttaa cgccaaaaca tcattcacac cgtcgcctgt cattgcaacc gttctgccgc
    12301 tttctttcaa ttcatttacc agcaatttct tttgctgcgg actcactcgt ccaaaaaccg
    12361 tatagcgatg agcagcttct cgcacttcca tttcttcagt aatttgagaa agatcgacat
    12421 acgcatcata attcggtaat cctgcacgtc tagcgatatt ggagacagta aaaggattgt
    12481 ctccagaaat cactttcaga tccacacctt cctcatgaag ataagctagt gtttcttgag
    12541 cgttttttcg gattggatca tcgatttcaa acagagcaac cggctcaaca aatgctggtt
    12601 tttcttcgtt caattgctga tttggagcga tccccaacat aagtacgcga tagccattcg
    12661 cttgggctgt ttttagactg tccggaagcc gatcttcttc caccaaacgt tcaggcgctc
    12721 ctacgtacac gactcccagt tctggaaatt ccatcgcgcc ccatttgcga tcagaagaaa
    12781 atgcgacttt gtttgtcaca tcataccgat tagaaagctc aaaataatct ctcatagccc
    12841 gcatagtgat gttgttatca ctgctttctg ccagatacgt ccccataatt tctgggatgg
    12901 ctttctcgta cagcggatgt agtgcttcta cccgctggat actcattttc ccttcagtaa
    12961 tggtccctgt tttatccaag cataatgtat cgacatgggc cagcgtttca attgaataca
    13021 tgtcttgaac aaggattcgt ttttttgcca gtttggttac ccctgtcgtt aatgcaatac
    13081 ttattagcaa taccaaaccc tttggaagca tgcctaacaa agctgctgca gatgcaataa
    13141 cagaaatttt gatagaagca tcgcggatga acagtgcttc caaaaatagt atgatcccca
    13201 aaggaataat cacgaaacta gtgaatttgg atactttgcg aatcgaatct acaagttcag
    13261 aacgaatagc tttgtgagtc tttgcctcgc tagtcaagcg gacagcataa ttatctgcac
    13321 ctactcggat aacttcagcc atgacagaac cgcttgtcaa aaagcttcca gaaagcaatt
    13381 cagctccagt ttctttgaca atcaaatcag attcgcctgt caaaagagct tcatttgctt
    13441 ctgcccgtcc agatataacc tgtaaatcgg ctggtatctg ttctcctgct ccgattttta
    13501 ccaaatctcc taataccagc tctttagtag aaatcgattg ttctttaccg tcccggatca
    13561 ctagtacttc tgattgggaa acaatcgata atttccgtac aagatttctt gcatgtattt
    13621 cttgataaat tccaataacg atattgacga caataattgc taaatacgcc atgttggaat
    13681 acgcacctac aaataacaga caaatcccaa taccaaagtt cagaaaattg aataacgtca
    13741 atatattatc gctgaatatt tgttttgtac tttttgatgc atcttcctgg tagtcatttt
    13801 gctggccaga agccattttc tgaactacct cttgctgtgt aagtccattc attttctttt
    13861 tctccatttt ccgccaactt tcaaaaataa tcacgtgttt aagtctagcg tgttccaaga
    13921 ttaaaaacaa cttgttttta taaaatttat agaaaaacac acgaatgatt ttacaaaaaa
    13981 gatgccaacc tttttgtttg aatcacccgt gtgctgtttt tattatcaat ctatctttac
    14041 ttatattgtt cctccaaacg gctcatccac caacctagtg ctgtaccggc tataaaaaga
    14101 acaggacaag ctagtacccc agcaactccc aatcccgcgt aatcggtagg aatgatcata
    14161 atcgttgaag caaaaactac acctaaaatg aaatggaata gcttgctata tgcctttgag
    14221 aaaatgtaat ccatgatttt tgacaatcca agaacacaaa gaatcccacc aattccgatc
    14281 ggcacaatca cttcaagtcg tacttcttta aatccgtcag ccatcgcttt atacaatccc
    14341 atatacacca aaaaattaga agggctcaat cctgggacga tcatacccaa accaatcaat
    14401 ccgccggcaa tcagccatgt tccaaaattc tgatcgactt gcccgtcaaa taattgagcg
    14461 ccgtataata aaaacaaata agccagaaca aaagtaacaa ccatgatgat cacatcagaa
    14521 gtatctcttc cttttttccc tgcttctttc cataacgcag gaacagttcc agcgatacat
    14581 ccgacaaaaa accataatat cgttgtttca tacgcaccta ataaaaaact gactaaaaaa
    14641 gaaagtagaa agatgcctgt cactccgcct agaccaacag gcagaaaaaa caaaacattt
    14701 tctttgaaat tttttgtaag gtgggctaaa aaagaaatca gccgctcata catgccaaag
    14761 acagctgcta atgcaccacc gctgactcct ggcaagataa atcctgaacc gataaacatt
    14821 cccttcacaa accgtaacat ccagtctttg gttgtgtacc ccacttcttg attttccatt
    14881 ttttgccttt tttccactat ttgcgtactc cttctttcat ttaattttat tcatgattga
    14941 acatttcctg ctattttact taaagacttt ttcataggtc tctattattt tttcattcat
    15001 tttgacaata tcaggataaa acatgttacc tccattatga tagagatagc cgccattgta
    15061 aagcaaggac tggatccgcc aatatcggta ttgtttccct tcctcatttc ctaaaagtgg
    15121 gctcagcaca tctttagaat acgcttctgc taattctact gtgttttctc cgccatgatt
    15181 ttttacatag gcaatataat ctttcccaaa attatatgct tggacagctg tccataaatc
    15241 gcatccttgt ttctttgctt ctactatcat ttccgccaaa tatttgacac cttgatcaat
    15301 actgtcagct ggattatcaa tgatctcagc ttctccatgc agactttccg agctctgcat
    15361 gagatctgtc ccttttcctt ttgattcggt caatattatc gccttgatca aattctcgta
    15421 gttttccata tgataatttt ttgcagctgt tgttatttct atttgatagc tttccactcg
    15481 ttttaccgaa cgataagttc ttcctcataa aaacaacacc gcaccaataa ccgtcagaat
    15541 gatcatgtaa aaaagaatac ggataataga taatcgtttt cgcttcattt catcacctag
    15601 ttattttgac atcttttttt atcttactat ttctttatct tcttggctat ttcttccttg
    15661 atttctgtgt acgccttaag aaaaaatgaa ttttcctgta ttttttgaca tcttacgaaa
    15721 atgatcgttg tttttcagta aaaacaattg cccaatgact gatataatcg ttatacttta
    15781 cacagtaggt gattatgtga aaaaagaaaa gaaatatgca aaaatgccag atgggagcga
    15841 aatttactat gaaaagagcg gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg
    15901 cagcggccgc tttttttcag aacaggtccc tgtactcgaa cgctactata ccgtgtatct
    15961 agtagatagc cgaggacatg gtcgttcgac aaacgaagca tctatgttga attttcagtt
    16021 aatggccgag gatctgaata cgatcatgtt gctggaaaaa atcgatcagg cagattttct
    16081 tggatttagt gatggagcaa atctagcact ggtatttgcc agcagcttcc ctaaaaaagt
    16141 tcatcgattg attttaaatt ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag
    16201 agtcatcagt aatattcatt atgcatgggt atggttactc tctttatttc gcccaagtct
    16261 tcgcaaaaat cttttagtta tcaaactttt gctgcatgac atcgggctta cagaaaatga
    16321 tttaaaaaaa atcaactccc ccactttgat catcgtggga aaaaaagatg taatcaagct
    16381 aaaacattct ttgtatatag ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga
    16441 acaaggacat gaattagcta gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt
    16501 tctttctgaa acataactat taggaataga aggtgattta tttgttaaaa aataccatac
    16561 aatggctgaa aaaccaccta ggactgttca aaacaatctt tttgatttct gtcattgtca
    16621 ttattgtcgg tgaattgatg tccatcggta aaactctatc tatccagcaa ttaggagaaa
    16681 cgtttgccac tatacctgta tggaaaagcg gactaatgct attgattggt cttatctctg
    16741 tgttacctat ggttggttat gatattattt tgaatcggat gcttgaacaa aagcagaatc
    16801 ctcgttacct gtttgagaca agctggctga tcaatacgat caacaatatt gccggtttcg
    16861 gcgggtttgt tagtgtgggg cttcgctctg aactatatgg acaaaagaaa gagagcagaa
    16921 acgtgatcca agcattatcg aaagttttct tgtttttgat ggctggactt tctatttaca
    16981 gtctgctttc ttttttactt gtagtcttca cgccagttgc tccttttttg aagcaatatt
    17041 ggatatggtt gatcggcggc ggtctatatt ttcctgtcgt ctttttattt actactttca
    17101 aaaagaaagg attccttggt ggtacctcac ttaaaactca aggacagcta ttgctagttt
    17161 cattgttgga atggaccggt gtcctgttta gtttcctttc tgttggttat ttaatggaaa
    17221 tccggattga tctgtggcaa acaattccat tattcattgc tgcttcagtg ataggaatcg
    17281 tgtctatgat tccaggagaa attgggagtt tcgatgtaat gatgattatc gggttgtccg
    17341 ctattggtgt gccaagagaa atagtcgttg tgtggatctt gctctatcgc ctgttctact
    17401 acatcattcc gtttttgatt ggaatcgtct ttttcttcaa aaatatcggt tccacttttg
    17461 atcagcgtta ttctggtatc cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg
    17521 ttgtcttatt atacttttct ggaatcatgc tagtactttc tgccactatc ccgcaagctt
    17581 ttacagagtt tcgctggctt catagtttga acccattgaa atttcatttt attatccaat
    17641 ttcctagtat actattggga tttcttttaa tcattatggg cagagggatc gcagcacgtg
    17701 tgaaacgagc atatttgcct acaatcttct tgattgttct agctttactt tatgttttat
    17761 taagtgattt tagtttcaca ccggtggttt tcttgtctat tctgcttctg atcattcttg
    17821 cttctaaaaa cgaattattt cgggaacaat tgatctattc atgggagtgg cgtacgatcg
    17881 atggaatcat tattggtgca ctgtccttgc tttacatcgt tattggggta tacaatctgc
    17941 cggatttccc tcatcggcgt caccatttta tttccttttt cttatttcct tctgaaaaga
    18001 tttggttttc gggattgttg gccatcatcg ctgttagttt catgatcgtt ctatttgttc
    18061 atttccttca aggagaaaag aaacagattg gcgaagcctt taatgaagaa aaggccctga
    18121 agatccttac aacttatgga ggaaactccg atagtcaatt gatcttctta aaagataagc
    18181 ggatgtttgc ctatgaaaaa gacggtgaac caactgttct tctccaattc gcttgcttca
    18241 ataataaatg tattgtaatg ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa
    18301 tcgaagcttt tatcgaagaa acagatcgac tatgctattt accagttttc tacgaaacaa
    18361 gcgaagagat tgttatgatt ttgcatgaat tcggctacga tttcattaag atgggggaag
    18421 aagcttatgt agacctgaat agttttacaa cttcagggaa aaaaatgaag ggcactcgtg
    18481 ctgtcttgaa tcggatcgaa cgagaaggat ttacatttga tgtcttacaa ccaccatttt
    18541 ctactgagca aatgagtact ttcaaaaata tctctgataa ttggttgggt tcacgaaagg
    18601 aaaaagggtt ttctttaggc tttttctctg aagattatct acaaagagcg cctattgcag
    18661 ttgtcaaaaa tacccaagaa gaaattgtcg cttttgcaac gatcatgcct acttacacga
    18721 acaataaagt aggaacgatt gatctgatgc gatatgattc taaaaaagca ccttctggca
    18781 gtatggattt cttgttcttg catttgtttg cctatatgaa agaagaacat atccaatggt
    18841 tcaatttagg gatggcccct ctttcaaatg tcggaacttc cagaaagagt tttttacagg
    18901 aaaggattgc atacttggtt tacgagtttg gttctcattt ttattctttc catggattaa
    18961 gagaatataa aagcaagtac gctacaaact gggtctcacg ttatactcta tattctagag
    19021 atagttggat tgcatatgtt atgattgctt tgctgattat tgataatgcg ccagttgagc
    19081 agacacaagg tagaataagt ggtttgaaaa aatggatacg aagaaagtat taaaaactgc
    19141 ataccgatag atgaacactc tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca
    19201 tgcttctaca atctcttctt atacaattga agaacaaaga gcggtccaaa cagaaatcct
    19261 gtttggaccg ctcttttatt ttaaacattt ttcattacca aaatattcga gtcgcgtttc
    19321 ttatgcttcg atctctgtaa cgatacctga acctactgtc cgtccgcctt cacgaattga
    19381 gaatgtcgtt ccgttttcaa cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt
    19441 gtcaccaggc atgaccattt ctgtatcttc tggcaatgtg attgttcctg taacatcagt
    19501 tgtgcggaag tagaactgtg gacgatagtt gttgaagaat ggagtatgac gtccaccttc
    19561 ttcttttgtc aacacgtaca cttctgcttt gaattttgta tgtggagtaa tggaacctgg
    19621 tttagcaagt acttgtccac gttcgatatc gtcacgttgg atcccccgta acaacacgcc
    19681 tacgttatcc ccagcttccc cgtaatctaa cgttttacgg aacatttcta cacctgttac
    19741 aactgctttt tgtgtttcag gtttgatccc tacgatctct acctcatcac cgacacgaac
    19801 agcaccacga tcaatacgac ctgaggcaac agttcctcga cctgtaattg agaagacgtc
    19861 ttccactggc aatagtaatg gtttatctgt atcacgttct ggtgttggga tatattcatc
    19921 tactgtatcc atcaattcca taatagcagc ttcagcatct ggatcgcctt gcaatgcttt
    19981 taatgcggaa cctttgatca caggagtgtc atcgcctggg aaaccatatt cgctcaataa
    20041 ctcgcgaact tccatttcta ccaaatcgat caattcttca tcatcgacta aatcaacttt
    20101 gttcaagaaa acaatcaggt attttacacc aacttggcgt gacaataaaa tgtgttcacg
    20161 tgtttgaggc atcggaccgt cagttgcaga aacaactaag attgcaccat ccatttgtgc
    20221 tgcccctgta atcatgtttt tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta
    20281 gtggcgtttt tctgtttcat attctacgtg tgcggtattg atcgtaatcc cacgttcacg
    20341 ttcttctgga gctgcatcaa tactagcata gtcttgagga tttgctaggc cttttttgcc
    20401 taatactgta gtgatcgcag cagttaaagt agttttccca tggtcaacgt gtccaatcgt
    20461 accaatgtta acgtgtggtt tacttctgtc ataatgttct tttgccataa tttacaaacc
    20521 tcctaaataa tatagatcac tgaaaaattt cagctctcac ctttttatta tagtcttttt
    20581 ttgactaaat caaaaagaat acgtccgact tactttttgt aagattcgaa ctaagcttat
    20641 tatacgtcaa tattggtcaa agtcaactaa gagcactcgt ctgtcttata cgcgaaaaaa
    20701 gtacctttta taaataaaaa aatgtgtttt ttgagaaaaa cttcaagata gtccttaact
    20761 ttcttttgaa gcaataagaa caaaatgagg aaaagtttaa aattcagctg ttgccaacaa
    20821 ctttcaatag acgaaatctt ctatttcgtt aataataagg aaagaggtga agtacatgaa
    20881 aaaattaatt ggcgtacttg tcgttttagg catatttctc ggcggaagtt ttgcagctta
    20941 tcattatttt tacggcggcg aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc
    21001 agctacccaa gcagataacg gagagaaatt cacgacttat acctacaagc aagcggctta
    21061 taataaagat ggtgaaaaaa aacaggtgac gcttcgcgaa gaaagagaac atccattaaa
    21121 gatgaatgcg tatcttaaac tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga
    21181 aataaaggct tctgaagtgc ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt
    21241 cttaaaattt tattagataa aaagaggctg ggacagaagc gtttaactcc aagaaataag
    21301 aaggagttgc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
    21361 ttacttttgt ctcacgctct ttttttgtta tattcgtctt ttccgatcct tctcttgaca
    21421 caattcattt aactgatcac tggtacttga acacctaata ttctagtcat ttttataaac
    21481 gattcaatac ttcatccaca tttttgatca tcacggatat cgtaccatcc ggattattca
    21541 caaattcaat caaatctggg ttgcgatata catctactgg tacgatcagt tcgatcccgt
    21601 ttgatagctt gaatttttgt tttccaaatt ttttctcaga aatttcttgt acttctttta
    21661 caggaggtgc ttctgggaca aatccttgct cttgtacttc ttctttaaaa gccatttttg
    21721 ccgtgatgtt ttccttgaag actttttccg caatcatctc atgatccaac cgaccttttt
    21781 cttctatcgt atcatagaca gcttctttca aatcagccat gatatcaaat tcttctgtct
    21841 caaatttttt tccaagatgc ttggctactt tttggatcac ttttacattt tcttctaaag
    21901 aaggtgcagg aacactttcg atcactcttc cagaaaaata aagctctttt ttgccagaaa
    21961 actcatattt tttttcaatt aattcgtagg tccaatcagt caaattcaca gcgaatgctt
    22021 catctgcttt ttgtgttttc gcccctagaa ttgctcgatt caaaatcaag cggttgtcga
    22081 tccctgcttc gtctgcttca acaaagtgag tgtaagcctc acgataattg actttcaata
    22141 aagataagta catcactgta tctaattcat acaagacaac aaatacatct gcactaggtg
    22201 cttcttcact ttcttggtag atatcgaacc accgctgaac gattgcttca cttgcttcca
    22261 caaaattttc ttgagcgatt ttaagtgatt caacgatcat gctttcttct gctagttgtc
    22321 cagtttttgt ttgagcagaa gagatttttt ggatcttctt ttgaagaaaa tcacgaatat
    22381 attcttttgt cagatccagc tctttttgtg aaaagactgg agaaccagtt tctctgtcaa
    22441 taatatgcag aattgctttt tttaaataga tatccatttt ctcattccct tctgtcttag
    22501 tttacggaaa aaaaatcaaa aataccagaa tcaaaatcat tttcttttaa acaaaaattt
    22561 caagagtaga aacaaaatgg attctttttt tcgaaaaatg cgctacactt atagttgaac
    22621 gaataaaaaa ggagctgaaa gtatgaattc attaacaagc acttatcgtc tatcaaatgg
    22681 ttacgaaatc ccggtagtag gattcggcac atggcaaaca cctgatgggg atgtcgctgt
    22741 ttcctctgtt aaagaagcgc tggctgcagg ttatcgccat atcgatactg cccaaggata
    22801 caagaatgaa gaaagcgtcg gccaagcgat taaggaaagt ggaattccta gagaagagat
    22861 attcttgaca acaaaattat ggaatgcaaa ccacagctat gaacttgtga tgtcttcttt
    22921 tgaagaatca ttgaagaagc tccaaacaga ttatttagat ctgtttttga tccactggcc
    22981 aaaccctgtt gcttttcgtg ataattggga acaagcaaat gcagatacat ggcgagcatt
    23041 tgaagaactg tatgaagcag gtaaaatcaa agcaatcggt gtgagcaatt tcttacccca
    23101 ccatctagat acactagcaa aaacagcaaa aattatgccg atggtcaatc aagttttcct
    23161 tgcaccaggc gaattacaga caactgtagt tgagtatgca aaaaaacatg atatgatcct
    23221 cgaagcatat agtccattag gcactggtaa aatcttcgat gtgcctgaaa tgaaacaaat
    23281 cgctgaagca catgacaaga cgattgcaca agttgctttg cgttggtcgc tgcaacacga
    23341 attccttcct cttcctaagt cagtgacacc aagcagaatc aaagaaaata ctgaattatt
    23401 tgattttgaa ttaacagaag aagaaatgaa acaaatcgat cagttagatg gtgtagtcgg
    23461 caaagcaaaa gacccagata ctacccaatt ttaagtcgaa aggtgatttt atggatccaa
    23521 tcgaacgctt gaattctctc tcggaagaag ttacccagac ttttcattcc gattttgtct
    23581 ttttgatcga tgcagagaaa atccaacatt ttcctgcaag aaactggaca catgatcaaa
    23641 tcatcgaaga actgaaaaaa cggttcgatc attcgctaat ggtcaaaccc tggcacgaac
    23701 atgaagtcat ttattcacct gaactacctg tttttgcctt gataccgaaa aaatagaaag
    23761 aatggaacgg attatgtata tatacataat ccgttccatt ttttattttt ctttcaaaaa
    23821 acgggaaaat acgcaaattt ttctgttgta atttgcgtat acgcaagttt ttttgcatta
    23881 ctccttttca tttggctgtt tttttcttat aatcgaaagg gtatgagcga aggagagaac
    23941 atatgaagaa aaaacagaaa caaaaattat taaatcaatt ccgtccttca ttggatggtg
    24001 taagaggaca attattccgt tctctagaag aagagagctt gcgacgttat ggtttgccgt
    24061 tacaagtcat gcttgatccc aaaaaccgtc aaatcttaac gatcggatta gctgggaaaa
    24121 caaatgaaga tacacggatc aacgtgccaa tcgatgacaa cttcaatact gtattaaaga
    24181 gaattcgttc aggagaaaac gggattttcg aacgattccg tgacaatctt cttattgaaa
    24241 tcgtgtctta ctggaatgat caacgattga aaaacgatga accaactgcg caagctgttg
    24301 cgacaccagt tacagaagaa acagtcagtg tgccagaaga aaaaacggaa aaaccaaaag
    24361 cggacacaac tgaaaataca cgctcaactg atacgaatgc cgctttaagt tttgctgatt
    24421 tttcaaaagc cgttgctgag tatcctaaat tttacacaga acaaaatgaa catgaagtag
    24481 ttattaatga aaaaaatgct gatgaacctc gtaaattagc aactatttct atgactactg
    24541 aaaatgagtt cgttatcgaa aaagcactcg aacggaaata caaagtcaaa ttgactttga
    24601 ttcctttgat cgaacaattt gcagcgacag cgattactga tcgataaaaa ttctatttgt
    24661 cataaaaggc tgggatttaa aatcccagcc tttttagtca tataataatt tctacagcaa
    24721 gtaaaaagaa attccttcta acatcaggct caaatatcag cttgacctgt ccaacaaatc
    24781 acctatgatc ctacaatgtt aggcgacttc tcttctacct gaagatatct tggagttgcc
    24841 tgatttctta agatgcctaa ccttaactat aaaaaattct aataataaac atattttctg
    24901 atagagaaaa aa
//
LOCUS       DACCZY010000031        24382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCZY010000031 DACCZY010000000
VERSION     DACCZY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.031"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..837)
                     /locus_tag="IVZ67_001041"
     CDS             complement(<1..837)
                     /locus_tag="IVZ67_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8165130.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="IVZ67_001042"
     CDS             complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="IVZ67_001042"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8165131.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3338..3517)
                     /locus_tag="IVZ67_001043"
     CDS             complement(3338..3517)
                     /locus_tag="IVZ67_001043"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8165132.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3564..5387)
                     /gene="cadA"
                     /locus_tag="IVZ67_001044"
     CDS             complement(3564..5387)
                     /gene="cadA"
                     /locus_tag="IVZ67_001044"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8165133.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5665..7944
                     /locus_tag="IVZ67_001045"
     CDS             5665..7944
                     /locus_tag="IVZ67_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8165134.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8037..8684)
                     /locus_tag="IVZ67_001046"
     CDS             complement(8037..8684)
                     /locus_tag="IVZ67_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165135.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8863..9582)
                     /locus_tag="IVZ67_001047"
     CDS             complement(8863..9582)
                     /locus_tag="IVZ67_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8165136.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9713..10864)
                     /locus_tag="IVZ67_001048"
     CDS             complement(9713..10864)
                     /locus_tag="IVZ67_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8165137.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11081..11821)
                     /locus_tag="IVZ67_001049"
     CDS             complement(11081..11821)
                     /locus_tag="IVZ67_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8165138.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="IVZ67_001050"
     CDS             complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="IVZ67_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8165139.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12205..12798)
                     /locus_tag="IVZ67_001051"
     CDS             complement(12205..12798)
                     /locus_tag="IVZ67_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8165140.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12782..13429)
                     /locus_tag="IVZ67_001052"
     CDS             complement(12782..13429)
                     /locus_tag="IVZ67_001052"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8165141.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="IVZ67_001053"
     CDS             complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="IVZ67_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8165142.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="IVZ67_001054"
     CDS             complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="IVZ67_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8165143.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14875..15402)
                     /locus_tag="IVZ67_001055"
     CDS             complement(14875..15402)
                     /locus_tag="IVZ67_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8165144.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15582..16367)
                     /locus_tag="IVZ67_001056"
     CDS             complement(15582..16367)
                     /locus_tag="IVZ67_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8165145.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16360..17229)
                     /locus_tag="IVZ67_001057"
     CDS             complement(16360..17229)
                     /locus_tag="IVZ67_001057"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8165146.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17230..18609)
                     /locus_tag="IVZ67_001058"
     CDS             complement(17230..18609)
                     /locus_tag="IVZ67_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8165147.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="IVZ67_001059"
     CDS             complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="IVZ67_001059"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8165148.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19061..19537)
                     /gene="accB"
                     /locus_tag="IVZ67_001060"
     CDS             complement(19061..19537)
                     /gene="accB"
                     /locus_tag="IVZ67_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8165149.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="IVZ67_001061"
     CDS             complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="IVZ67_001061"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8165150.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="IVZ67_001062"
     CDS             complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="IVZ67_001062"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8165151.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="IVZ67_001063"
     CDS             complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="IVZ67_001063"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8165152.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22567..22791)
                     /locus_tag="IVZ67_001064"
     CDS             complement(22567..22791)
                     /locus_tag="IVZ67_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8165153.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22844..23806)
                     /locus_tag="IVZ67_001065"
     CDS             complement(22844..23806)
                     /locus_tag="IVZ67_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8165154.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23816..24256)
                     /locus_tag="IVZ67_001066"
     CDS             complement(23816..24256)
                     /locus_tag="IVZ67_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8165155.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6666 a         5431 c         4246 g         8039 t
ORIGIN      
        1 cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt
       61 tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca aaagtgaatt
      121 agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga gtacatcggc
      181 tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga tattcatgct
      241 tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg aaacaagcca
      301 aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga tatctttttc
      361 aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta ctactaaaga
      421 agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact ctacgacttt
      481 ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt gtgcattgtt
      541 accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac taaaaccttc
      601 atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca gactttgaaa
      661 accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag cttctgctaa
      721 ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt tggccaactt
      781 tttttctgct gaagaataat cttccatcat actttcaata cgactaatta cgctcataag
      841 ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc ttattcacgg
      901 tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt atcccatcaa
      961 tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct attgttcgga
     1021 ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct ctgctgtaat
     1081 ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca gctgtttctt
     1141 tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct ccaacaacta
     1201 attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt ttttcattac
     1261 gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt tcataagcat
     1321 aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt gccaaccatg
     1381 ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc attgtagaaa
     1441 caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct ccaacatatg
     1501 tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat gtttcttctg
     1561 taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt
     1621 ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt gcttgggctt
     1681 tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg cgatagctca
     1741 agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc aattcaagcg
     1801 tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca tcattcaacg
     1861 tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag cgatgcatca
     1921 ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg tttttgtaga
     1981 ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca tctcccatat
     2041 ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt ttgtacaact
     2101 caacatctcc cattattggt gtatatacat gttggtaacc tagactaact tctttgtcta
     2161 cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag aatggcaaac
     2221 cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt ttacgatggt
     2281 ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc ttgtcaaaaa
     2341 aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc caataagcac
     2401 ctgccactga taataattta aatacttgta tacggccagt ggatggaaca tgtggtccac
     2461 gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct tctggcaatt
     2521 cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct tcctgttttg
     2581 aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt tctgcttcga
     2641 ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg tagtaaaacc
     2701 ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga cgcatggctt
     2761 gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca tggtctggtg
     2821 tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg agttttccgt
     2881 tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca gcaattgcca
     2941 atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta atttttatca
     3001 taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa
     3061 atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc acctaatgtt
     3121 caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct tcaacaaggg
     3181 tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag cttttcctct
     3241 cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt gtttatttaa
     3301 ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat tccccattta
     3361 caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct tttggtgcct
     3421 gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat tgttccggac
     3481 tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct ctttctgatc
     3541 gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga atcaggaaaa
     3601 atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc cacgacttct
     3661 tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa
     3721 atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt atctcttgaa
     3781 atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc atctgcactt
     3841 tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc agcagcaagt
     3901 gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc tttagggata
     3961 ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga atctaattgg
     4021 actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat catcaaattt
     4081 ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg aatctgatcc
     4141 gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc tgtattggac
     4201 gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc atgattattg
     4261 actactgctt ggattccttg accggtcact tctttcagtt gactaacagg atacagttgg
     4321 ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc ctgttctgca
     4381 ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg aatcgcctct
     4441 acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt
     4501 gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact tgaacggctc
     4561 attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga tgccactacc
     4621 agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc accgatcaaa
     4681 taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa tcgggcaggc
     4741 ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc tgcggcacgt
     4801 ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat aatctgatct
     4861 ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc gaccgaagat
     4921 tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac tagtaactgg
     4981 tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt gaatcgatgg
     5041 gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg tttattcgcg
     5101 taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact agcccagtat
     5161 tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac gccatatctt
     5221 cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat ccctccagta
     5281 aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact taataaagct
     5341 atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt tttttctcct
     5401 cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc atcttaagca
     5461 tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac tatcactatc
     5521 agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat tactttttcg
     5581 attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta attcgttatt
     5641 tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac gcacgtggat
     5701 gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca acaagaattg
     5761 actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg agctggttcc
     5821 aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga attgcttttg
     5881 aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat
     5941 agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac attatatcta
     6001 ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg gcgcgcacca
     6061 attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac tgaaacagat
     6121 agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg acgtatttta
     6181 tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat attggatgaa
     6241 tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc
     6301 attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag tggaaagaca
     6361 tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg gctcgaggct
     6421 gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc gactgtattg
     6481 ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt ggtgcagctg
     6541 ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt gctaggggaa
     6601 aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat tgatcgctat
     6661 atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa tggccgtacg
     6721 atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca attaccttta
     6781 catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa
     6841 gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca agaactatac
     6901 gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg taaaaaactg
     6961 gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat gaattatcaa
     7021 tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact ttctcttcta
     7081 acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt tcgtacacac
     7141 ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact gatgcgccga
     7201 acatatccag tgacggttac acacaaagct cgctatatct ttattgatga aatgcaggat
     7261 ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa tttgactttc
     7321 tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc attggaagac
     7381 cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg ctccacaaaa
     7441 gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga aacaacagca
     7501 agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa attgaattgg
     7561 ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac agcaatcata
     7621 gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga gttgaaacaa
     7681 caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat tttacttcca
     7741 gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt agatgcagaa
     7801 catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg tgcgatgcat
     7861 gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat ggatatttca
     7921 tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat gttagcagtt
     7981 gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa ggtcatttat
     8041 tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga aataatcatt
     8101 ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg acggccaatc
     8161 ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc aattgattgg
     8221 aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa tgctaggtat
     8281 gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc gataagaatt
     8341 ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc catccgatac
     8401 tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg aatcagataa
     8461 ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata acggttcaga
     8521 taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct tgcgtatttt
     8581 tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt gcggtaaccg
     8641 cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt ccccgtccat
     8701 cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac cacaggttga
     8761 attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga gattgtcttg
     8821 cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac
     8881 attatggaac acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa
     8941 tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc
     9001 gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc
     9061 tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc
     9121 tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc
     9181 tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc
     9241 aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat
     9301 caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc
     9361 tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt
     9421 agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt
     9481 tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt
     9541 agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct
     9601 gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc
     9661 tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc
     9721 ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat
     9781 cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag
     9841 aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa
     9901 gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac
     9961 aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat
    10021 cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca
    10081 tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg
    10141 gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg
    10201 gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg
    10261 ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat
    10321 gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat
    10381 gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca
    10441 ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat
    10501 cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg
    10561 ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa
    10621 aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat
    10681 tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca
    10741 aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt
    10801 ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt
    10861 tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg
    10921 caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc
    10981 gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga
    11041 agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa
    11101 agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga
    11161 ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct
    11221 cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa
    11281 aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag
    11341 caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg
    11401 tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa
    11461 acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa
    11521 ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg
    11581 atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta
    11641 accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg
    11701 ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt
    11761 gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca
    11821 tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca
    11881 gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga
    11941 tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt
    12001 cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac
    12061 agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt
    12121 ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga
    12181 tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat
    12241 tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc
    12301 aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg
    12361 aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat
    12421 cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc
    12481 taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt
    12541 caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg
    12601 ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta
    12661 ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc
    12721 gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt
    12781 actatagtcc atattcatac aacccttcat tttgaatata atcaattact ttatctggaa
    12841 caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt
    12901 cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc
    12961 gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac cattttggca
    13021 aatattctac catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg
    13081 tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt
    13141 caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg
    13201 catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc
    13261 ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg
    13321 gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt
    13381 cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc
    13441 cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt
    13501 ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata
    13561 cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt
    13621 ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc
    13681 cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc
    13741 ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta
    13801 caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac
    13861 cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa
    13921 atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa
    13981 attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa
    14041 taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg
    14101 tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc
    14161 gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat
    14221 caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa
    14281 actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa
    14341 gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt
    14401 cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata
    14461 catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag
    14521 tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt
    14581 caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca
    14641 cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat
    14701 ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa cgctgacaat
    14761 agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac
    14821 cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc
    14881 gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt
    14941 ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag
    15001 gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac
    15061 cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc
    15121 taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac
    15181 acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa
    15241 aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc
    15301 taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata
    15361 gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca
    15421 aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa
    15481 aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa
    15541 aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc
    15601 ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca
    15661 atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac
    15721 gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc
    15781 atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct
    15841 tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct
    15901 agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt
    15961 aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct
    16021 cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt
    16081 tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa
    16141 tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt
    16201 ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca
    16261 ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt
    16321 tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc
    16381 tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac
    16441 aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag
    16501 ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg
    16561 ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg
    16621 gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga
    16681 gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc
    16741 tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt
    16801 ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat
    16861 gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt
    16921 atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac
    16981 aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca
    17041 tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt
    17101 cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg
    17161 cgagttagca ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa
    17221 cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc
    17281 ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat
    17341 ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat
    17401 cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc
    17461 ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc
    17521 acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg
    17581 attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca
    17641 cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt
    17701 gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa
    17761 atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt
    17821 agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga
    17881 aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa
    17941 atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata
    18001 aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc
    18061 tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc
    18121 accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc
    18181 ttcttctact gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg
    18241 catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc
    18301 aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa
    18361 accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg
    18421 aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa
    18481 ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag
    18541 ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt
    18601 agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac
    18661 cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa
    18721 cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc
    18781 cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg
    18841 cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc
    18901 cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa
    18961 ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta
    19021 tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa
    19081 agagtggctg tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc
    19141 cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt
    19201 ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca
    19261 gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt
    19321 tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg actggctccg
    19381 aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat
    19441 tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa
    19501 attgactgac taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat
    19561 gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact
    19621 tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac
    19681 attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat
    19741 cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc
    19801 tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg
    19861 tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc
    19921 catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg
    19981 gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc
    20041 ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc
    20101 attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc
    20161 tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc
    20221 aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt
    20281 tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc
    20341 aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt
    20401 tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc
    20461 agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat
    20521 ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct
    20581 atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat
    20641 ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa
    20701 gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat
    20761 taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc
    20821 gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc
    20881 aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac
    20941 tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca
    21001 tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc
    21061 tttactagct gcataatttg cttgaccaat atttccaatc aacccagata cactagagag
    21121 attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt
    21181 gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat
    21241 cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt
    21301 ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac
    21361 tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc
    21421 gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc
    21481 gattcctctt gtactacctg tgataaatac attttttcct tttacatcca taacgttcct
    21541 ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt
    21601 attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg
    21661 acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc
    21721 atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg
    21781 ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt
    21841 ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc
    21901 attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg
    21961 ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg
    22021 ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt
    22081 ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc tactaacgca
    22141 actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc
    22201 agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg
    22261 ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta
    22321 aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt
    22381 acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta
    22441 aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc
    22501 ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt
    22561 taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc
    22621 gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa
    22681 atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt
    22741 ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata
    22801 ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa
    22861 tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta
    22921 aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg
    22981 tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg
    23041 caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt
    23101 ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc
    23161 tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat
    23221 tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt
    23281 tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca
    23341 acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca
    23401 ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa
    23461 atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt
    23521 gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa
    23581 aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc
    23641 ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg
    23701 aaatccattc atcagaggta tccatcatct tggaaagatc attattagta ataatctttt
    23761 caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact
    23821 tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct
    23881 ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc
    23941 gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac
    24001 tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc
    24061 caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga
    24121 ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct
    24181 cagactcttc gattgtcaaa atatcattga agacactaac aagataatca tttactgttt
    24241 ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg
    24301 ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata
    24361 tgagcttttt ttaagatttt ac
//
LOCUS       DACCZY010000032        24259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCZY010000032 DACCZY010000000
VERSION     DACCZY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.032"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVZ67_001067"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVZ67_001067"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8165156.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVZ67_001068"
     CDS             complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVZ67_001068"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8165157.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVZ67_001069"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVZ67_001069"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8165158.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="IVZ67_001070"
     CDS             complement(3373..4680)
                     /locus_tag="IVZ67_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8165159.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="IVZ67_001071"
     CDS             complement(4683..5264)
                     /locus_tag="IVZ67_001071"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8165160.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="IVZ67_001072"
     CDS             complement(5836..7128)
                     /locus_tag="IVZ67_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8165161.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="IVZ67_001073"
     CDS             complement(7125..7748)
                     /locus_tag="IVZ67_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165162.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="IVZ67_001074"
     CDS             complement(7751..8587)
                     /locus_tag="IVZ67_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8165163.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="IVZ67_001075"
     CDS             complement(8604..9491)
                     /locus_tag="IVZ67_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8165164.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="IVZ67_001076"
     CDS             complement(9507..10790)
                     /locus_tag="IVZ67_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8165165.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="IVZ67_001077"
     CDS             complement(11473..12156)
                     /locus_tag="IVZ67_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8165166.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="IVZ67_001078"
     CDS             complement(12174..12791)
                     /locus_tag="IVZ67_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8165167.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="IVZ67_001079"
     CDS             complement(12801..13625)
                     /locus_tag="IVZ67_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8165168.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="IVZ67_001080"
     CDS             complement(13618..14178)
                     /locus_tag="IVZ67_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165169.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="IVZ67_001081"
     CDS             14352..15308
                     /locus_tag="IVZ67_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8165170.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="IVZ67_001082"
     CDS             complement(15613..16569)
                     /locus_tag="IVZ67_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8165171.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="IVZ67_001083"
     CDS             16673..17275
                     /locus_tag="IVZ67_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8165172.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="IVZ67_001084"
     CDS             complement(17363..18862)
                     /locus_tag="IVZ67_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8165173.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="IVZ67_001085"
     CDS             complement(18878..19402)
                     /locus_tag="IVZ67_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165174.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="IVZ67_001086"
     CDS             19700..20449
                     /locus_tag="IVZ67_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8165175.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="IVZ67_001087"
     CDS             20440..21225
                     /locus_tag="IVZ67_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8165176.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="IVZ67_001088"
     CDS             21194..21910
                     /locus_tag="IVZ67_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8165177.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="IVZ67_001089"
     CDS             complement(22012..23031)
                     /locus_tag="IVZ67_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8165178.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24108)
                     /locus_tag="IVZ67_001090"
     CDS             complement(23050..24108)
                     /locus_tag="IVZ67_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8165179.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7624 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DACCZY010000033        24138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCZY010000033 DACCZY010000000
VERSION     DACCZY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.033"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            114..887
                     /locus_tag="IVZ67_001091"
     CDS             114..887
                     /locus_tag="IVZ67_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165180.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(968..1537)
                     /gene="thiT"
                     /locus_tag="IVZ67_001092"
     CDS             complement(968..1537)
                     /gene="thiT"
                     /locus_tag="IVZ67_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8165181.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1603..1705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1711..2682)
                     /locus_tag="IVZ67_001093"
     CDS             complement(1711..2682)
                     /locus_tag="IVZ67_001093"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8165182.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2776..4149)
                     /gene="glmU"
                     /locus_tag="IVZ67_001094"
     CDS             complement(2776..4149)
                     /gene="glmU"
                     /locus_tag="IVZ67_001094"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8165183.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4652..5470)
                     /gene="purR"
                     /locus_tag="IVZ67_001095"
     CDS             complement(4652..5470)
                     /gene="purR"
                     /locus_tag="IVZ67_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8165184.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5637..6461)
                     /locus_tag="IVZ67_001096"
     CDS             complement(5637..6461)
                     /locus_tag="IVZ67_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8165185.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6434..7114)
                     /locus_tag="IVZ67_001097"
     CDS             complement(6434..7114)
                     /locus_tag="IVZ67_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8165186.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7137..8075)
                     /locus_tag="IVZ67_001098"
     CDS             complement(7137..8075)
                     /locus_tag="IVZ67_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8165187.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8320..9171)
                     /locus_tag="IVZ67_001099"
     CDS             complement(8320..9171)
                     /locus_tag="IVZ67_001099"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8165188.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9325..11898)
                     /gene="mprF"
                     /locus_tag="IVZ67_001100"
     CDS             complement(9325..11898)
                     /gene="mprF"
                     /locus_tag="IVZ67_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8165189.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            12039..12845
                     /gene="proC"
                     /locus_tag="IVZ67_001101"
     CDS             12039..12845
                     /gene="proC"
                     /locus_tag="IVZ67_001101"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8165190.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12924..14480)
                     /gene="rny"
                     /locus_tag="IVZ67_001102"
     CDS             complement(12924..14480)
                     /gene="rny"
                     /locus_tag="IVZ67_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8165191.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14891..15940)
                     /gene="recA"
                     /locus_tag="IVZ67_001103"
     CDS             complement(14891..15940)
                     /gene="recA"
                     /locus_tag="IVZ67_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8165192.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(16049..17290)
                     /locus_tag="IVZ67_001104"
     CDS             complement(16049..17290)
                     /locus_tag="IVZ67_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8165193.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17530..18108)
                     /gene="pgsA"
                     /locus_tag="IVZ67_001105"
     CDS             complement(17530..18108)
                     /gene="pgsA"
                     /locus_tag="IVZ67_001105"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8165194.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18317..19201)
                     /locus_tag="IVZ67_001106"
     CDS             complement(18317..19201)
                     /locus_tag="IVZ67_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165195.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(19979..21265)
                     /locus_tag="IVZ67_001107"
     CDS             complement(19979..21265)
                     /locus_tag="IVZ67_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8165196.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(21258..22526)
                     /locus_tag="IVZ67_001108"
     CDS             complement(21258..22526)
                     /locus_tag="IVZ67_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8165197.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22778..23239
                     /gene="mscL"
                     /locus_tag="IVZ67_001109"
     CDS             22778..23239
                     /gene="mscL"
                     /locus_tag="IVZ67_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8165198.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(23298..23744)
                     /locus_tag="IVZ67_001110"
     CDS             complement(23298..23744)
                     /locus_tag="IVZ67_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165199.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23837..24082)
                     /locus_tag="IVZ67_001111"
     CDS             complement(23837..24082)
                     /locus_tag="IVZ67_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165200.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         6899 a         5221 c         4219 g         7799 t
ORIGIN      
        1 ttttcaatga aaacgtgatt ttgtaatgga attttaaaat aaaagaaaca agctttttta
       61 atgcaaatgt tgtaaaataa aagagattaa gaggaaataa gaggaggaac atgatgactg
      121 agtcagttta tgtacatatt gataccacaa gtaatgctgt tttaacaaaa gggctgactg
      181 ctactgactt tgcaaacagt atcgtgcatt ttcctcaaaa cttgctcttg cttgatccat
      241 ctgcttctgc tggagaatat gaatcccata cagggctgaa agtaatccgt ggtacagaaa
      301 atattcagcg tttcttttcc tcttctcgta acaggcgtgg attcgatgaa ttgaagtgga
      361 tcgactttac tgatttaacg atgctgaaag aattgacacc tttagaaatt tcagaattat
      421 tatattttgg tcatatgaaa acacatcttc attcgccgtt tttttataaa ctacagaata
      481 attttgtata cttcgatctt aatgatcagc tgaatcggat ttattatcgc tatttagatg
      541 aattttatag gatttttgct agtaaattaa gttcgatctt gtcagataag atcaatgtaa
      601 aaaaatcttt tttcaaaaaa gaaacacctg ttgaaaaaat cagtgtggaa ttattgaaag
      661 aaatgcgtaa tttgatgcag gaagggatca ttttttcttt tgatcaagtt ggtttgatcg
      721 atggtaaata tcgtatccca atctatgtga ttgaagatta tatttggaaa ttaaaaggca
      781 cccggtatcg agaagaggaa atcatcgcta ctttaatcta ctctgtcacc gatcgacact
      841 ggcaattggt agaagatgcc gatctttctt atctgccatt gaagtagatc tatttcttat
      901 caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt tcttgcttct
      961 tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga
     1021 tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca ccattcatta
     1081 cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag aaaaccactc
     1141 ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca aacacgatgc
     1201 tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt gaagccatga
     1261 gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact tgaacgacac
     1321 ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca
     1381 tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa
     1441 tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct aacgctgcaa
     1501 caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct cctttgaata
     1561 aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt tattcacaat
     1621 tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc aatctcagcc
     1681 caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa acaatggact
     1741 aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc caacacttac
     1801 ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg taacaactag
     1861 acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata ctggatgtgt
     1921 acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat
     1981 cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt tgacttgtcc
     2041 aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta tgatcgcaat
     2101 cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat ggtctggtga
     2161 aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg caataagtgg
     2221 cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgga ctgcatgaag
     2281 gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc
     2341 tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat aataaggcat
     2401 gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga tcagcaactc
     2461 catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat gcgatccacg
     2521 aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa cagatgattt
     2581 tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat tggaatttaa
     2641 cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct ccactttctt
     2701 tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa taaatcaagt
     2761 gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt ctttattgac
     2821 ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc
     2881 ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg tgctagaacc
     2941 aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat agttgacgaa
     3001 gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa cataagtcaa
     3061 gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa agtttccgat
     3121 atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc
     3181 atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt gatcctcaat
     3241 cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt
     3301 tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc
     3361 gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca tctggttaat
     3421 tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga ctcccattga
     3481 ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt ttaagatttc
     3541 gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt tgcttaacgc
     3601 atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt gtacaagagc
     3661 ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa tatggtcacg
     3721 gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc
     3781 attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca ataatggtgt
     3841 atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag cagcttgcaa
     3901 aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc gttctcctag
     3961 ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt
     4021 ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg gatgtaaaac
     4081 tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa ttgcataacg
     4141 tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc ccagtatacc
     4201 cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga taaaaaaata
     4261 gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa ctcctcggtc
     4321 acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt
     4381 agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag ctgaccttcg
     4441 gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt gagctaatac
     4501 tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag ccagtttctc
     4561 ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact
     4621 actcgaaact aagcggcttt ttcacaacct cttattctga aaaataattt cctggcacaa
     4681 ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac gtataatcat
     4741 cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca acaagttctg
     4801 attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct ttcatgaaat
     4861 catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt tccatctttt
     4921 caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct tctgttattt
     4981 ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca gcttgggcaa
     5041 taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca aggtatttag
     5101 aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa tcagagagat
     5161 aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct agagattcaa
     5221 tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca cctgcagcac
     5281 ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg atgattccca
     5341 aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt gttaaggaaa
     5401 tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt tcactgcgac
     5461 gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt atataagttt
     5521 ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta tttattaaaa
     5581 taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag
     5641 ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa
     5701 gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt
     5761 agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag
     5821 cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt
     5881 gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc
     5941 gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca
     6001 tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa
     6061 aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt
     6121 gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc
     6181 attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat
     6241 atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag
     6301 aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt
     6361 cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca
     6421 agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca
     6481 ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta
     6541 gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg
     6601 attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa
     6661 aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat
     6721 ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt
     6781 cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca
     6841 ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta
     6901 atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta
     6961 atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga
     7021 caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt
     7081 aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa
     7141 ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct
     7201 gctttgattt gttcattagt caagctttct aaaggattca acacttctag cttcactcct
     7261 gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact
     7321 ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc
     7381 tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa
     7441 gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag
     7501 tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt
     7561 tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata
     7621 gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct
     7681 tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct
     7741 tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc
     7801 atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca
     7861 cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca
     7921 tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa
     7981 tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct
     8041 atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa
     8101 tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat
     8161 agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg
     8221 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
     8281 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
     8341 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
     8401 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
     8461 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
     8521 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
     8581 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
     8641 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
     8701 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
     8761 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
     8821 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
     8881 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
     8941 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
     9001 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
     9061 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
     9121 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
     9181 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
     9241 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
     9301 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
     9361 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
     9421 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
     9481 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
     9541 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
     9601 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
     9661 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
     9721 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
     9781 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
     9841 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
     9901 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
     9961 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
    10021 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
    10081 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
    10141 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
    10201 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
    10261 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
    10321 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
    10381 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
    10441 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
    10501 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
    10561 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
    10621 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
    10681 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
    10741 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
    10801 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
    10861 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
    10921 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
    10981 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
    11041 tagccaaact actgctacat cttgccgaac agacaattga ctcattccta agatcatcaa
    11101 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
    11161 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
    11221 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
    11281 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
    11341 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
    11401 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
    11461 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
    11521 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
    11581 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
    11641 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
    11701 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
    11761 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
    11821 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
    11881 gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca
    11941 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
    12001 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
    12061 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
    12121 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
    12181 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
    12241 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
    12301 tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca gcgcttgaag
    12361 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
    12421 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
    12481 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
    12541 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
    12601 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
    12661 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
    12721 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
    12781 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
    12841 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
    12901 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
    12961 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
    13021 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
    13081 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
    13141 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
    13201 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
    13261 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
    13321 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
    13381 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
    13441 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
    13501 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
    13561 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
    13621 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
    13681 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
    13741 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
    13801 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
    13861 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
    13921 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
    13981 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
    14041 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
    14101 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
    14161 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
    14221 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
    14281 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
    14341 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
    14401 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
    14461 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
    14521 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
    14581 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
    14641 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag
    14701 tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg
    14761 aacagatatt tttattttat gtacccactc gtataattaa ataactcacg atttctttat
    14821 tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc gtttttgttt
    14881 atcataattt ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga
    14941 cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca
    15001 tttcaggatg attagccata tagattttag cgttttcccg tccttgacca atccgatctt
    15061 ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta
    15121 gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga
    15181 aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg
    15241 taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca
    15301 atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga
    15361 tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca
    15421 atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga
    15481 tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac
    15541 ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa
    15601 gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag
    15661 catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa
    15721 ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac
    15781 cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt
    15841 ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt
    15901 tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat
    15961 ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa
    16021 gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat
    16081 caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt
    16141 tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac
    16201 cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta
    16261 tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg
    16321 ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga
    16381 atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc
    16441 cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta
    16501 cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt
    16561 ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca
    16621 ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct
    16681 gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc
    16741 gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta
    16801 cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat
    16861 gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc
    16921 cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt
    16981 caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt
    17041 gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga
    17101 gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat
    17161 ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga
    17221 aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt
    17281 ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat atttttttat
    17341 ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt tccaaataaa
    17401 cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac atgaaaagct
    17461 gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa cctctccact
    17521 aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt aatccacacc
    17581 tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg gaataccgat
    17641 caggctgaat gggatattat tgataaacag caagataata gcaaccattt gtgtagccgt
    17701 cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa gtagtaaacg
    17761 caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac caggggcctt
    17821 tccttggtca accaacatga taaaagcagt cataactagc attttatctg ccaacgggtc
    17881 cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc catctaacca
    17941 gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga cagccagtgt
    18001 ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca aaatgaaaat
    18061 cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac tatcgactcc
    18121 tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt tacttttcca
    18181 aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt aacgaagaag
    18241 gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa acagatgcgt
    18301 aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt tttggactct
    18361 gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc gttgcattgc
    18421 ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg
    18481 tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc acacctaacc
    18541 atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct gtgatattta
    18601 ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt tcttcacttg
    18661 attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc tctgaggaag
    18721 aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg attattggat
    18781 cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc aaagcaacca
    18841 atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat tcttcctgat
    18901 gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg accgatcctg
    18961 tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct cctacagctt
    19021 cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat
    19081 cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt aattcatcta
    19141 aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca
    19201 ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc aacgaaagac
    19261 gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg aataaacatt
    19321 gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa gaagctgttt
    19381 tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc tttcttaaac
    19441 ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat aaggagatat
    19501 ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa
    19561 acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acattaagaa
    19621 gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca caacctcttg
    19681 gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac aaaaatttga
    19741 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    19801 tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt
    19861 tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag
    19921 gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt
    19981 attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg
    20041 ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat
    20101 cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga
    20161 aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat
    20221 catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg
    20281 tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc
    20341 tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa
    20401 tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg
    20461 gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca
    20521 atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct
    20581 ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct
    20641 ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg
    20701 tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat
    20761 gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt
    20821 cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt
    20881 ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat
    20941 ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc
    21001 cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta
    21061 aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt
    21121 cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt
    21181 taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga
    21241 tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc
    21301 aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc
    21361 aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg
    21421 attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc
    21481 tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc
    21541 agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct
    21601 cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc tctgacattc
    21661 atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa
    21721 cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg
    21781 attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga
    21841 tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg
    21901 tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga
    21961 tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct
    22021 gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa
    22081 gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca
    22141 aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa
    22201 aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg
    22261 atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg
    22321 ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt caaactattt
    22381 gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca
    22441 gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc
    22501 acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc
    22561 ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag
    22621 ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt
    22681 ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg
    22741 atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt
    22801 catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac
    22861 aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc
    22921 aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac
    22981 aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac
    23041 tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa
    23101 agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg
    23161 cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct
    23221 ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc
    23281 tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat atgtggaata
    23341 tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata aaaagcttgt
    23401 aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg gatcatgtta
    23461 atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg atgaacaagg
    23521 acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc aaggatcttc
    23581 ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc aacttcttga
    23641 taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac
    23701 tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca aacgccccct
    23761 gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc ttacgaatct
    23821 caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact gtccgagtaa
    23881 gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga tccaaatcaa
    23941 caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc
    24001 ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa tctttcttga
    24061 ttgaagctaa agttgttggc atacagttca cctctactat caactatacc acatattttt
    24121 aaaattttca agtttacc
//
LOCUS       DACCZY010000034        23829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCZY010000034 DACCZY010000000
VERSION     DACCZY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.034"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..787)
                     /locus_tag="IVZ67_001112"
     CDS             complement(53..787)
                     /locus_tag="IVZ67_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8165201.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVZ67_001113"
     CDS             1114..1524
                     /locus_tag="IVZ67_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8165202.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVZ67_001114"
     CDS             1517..2218
                     /locus_tag="IVZ67_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8165203.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVZ67_001115"
     CDS             2218..3804
                     /locus_tag="IVZ67_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8165204.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVZ67_001116"
     CDS             3823..4044
                     /locus_tag="IVZ67_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8165205.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVZ67_001117"
     CDS             4041..4802
                     /locus_tag="IVZ67_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8165206.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVZ67_001118"
     CDS             5182..5691
                     /locus_tag="IVZ67_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8165207.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVZ67_001119"
     CDS             complement(5766..6305)
                     /locus_tag="IVZ67_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8165208.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVZ67_001120"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVZ67_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8165209.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVZ67_001121"
     CDS             7372..8169
                     /locus_tag="IVZ67_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8165210.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVZ67_001122"
     CDS             8197..8832
                     /locus_tag="IVZ67_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8165211.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVZ67_001123"
     CDS             8852..9625
                     /locus_tag="IVZ67_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8165212.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVZ67_001124"
     CDS             10033..10830
                     /locus_tag="IVZ67_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8165213.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVZ67_001125"
     CDS             10808..11221
                     /locus_tag="IVZ67_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165214.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVZ67_001126"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVZ67_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8165215.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVZ67_001127"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVZ67_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8165216.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVZ67_001128"
     CDS             15998..16558
                     /locus_tag="IVZ67_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8165217.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVZ67_001129"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVZ67_001129"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8165218.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVZ67_001130"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVZ67_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8165219.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVZ67_001131"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVZ67_001131"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8165220.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVZ67_001132"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVZ67_001132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8165221.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVZ67_001133"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVZ67_001133"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8165222.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVZ67_001134"
     CDS             21905..22483
                     /locus_tag="IVZ67_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8165223.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVZ67_001135"
     CDS             22535..23017
                     /locus_tag="IVZ67_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8165224.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVZ67_001136"
     CDS             23134..23508
                     /locus_tag="IVZ67_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165225.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DACCZY010000035        23653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCZY010000035 DACCZY010000000
VERSION     DACCZY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.035"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..601)
                     /locus_tag="IVZ67_001137"
     CDS             complement(26..601)
                     /locus_tag="IVZ67_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8165226.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="IVZ67_001138"
     CDS             complement(606..1580)
                     /locus_tag="IVZ67_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP8165227.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="IVZ67_001139"
     CDS             1863..2831
                     /locus_tag="IVZ67_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8165228.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="IVZ67_001140"
     CDS             2928..3221
                     /locus_tag="IVZ67_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8165229.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="IVZ67_001141"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="IVZ67_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8165230.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3687..4433
                     /locus_tag="IVZ67_001142"
     CDS             3687..4433
                     /locus_tag="IVZ67_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8165231.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="IVZ67_001143"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="IVZ67_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8165232.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="IVZ67_001144"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="IVZ67_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8165233.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="IVZ67_001145"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="IVZ67_001145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8165234.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="IVZ67_001146"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="IVZ67_001146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8165235.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="IVZ67_001147"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="IVZ67_001147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8165236.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="IVZ67_001148"
     CDS             9170..9712
                     /locus_tag="IVZ67_001148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8165237.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="IVZ67_001149"
     CDS             9735..11291
                     /locus_tag="IVZ67_001149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8165238.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="IVZ67_001150"
     CDS             11305..12207
                     /locus_tag="IVZ67_001150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8165239.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="IVZ67_001151"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="IVZ67_001151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8165240.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="IVZ67_001152"
     CDS             13650..14072
                     /locus_tag="IVZ67_001152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8165241.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="IVZ67_001153"
     CDS             14541..14771
                     /locus_tag="IVZ67_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8165242.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="IVZ67_001154"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="IVZ67_001154"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8165243.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="IVZ67_001155"
     CDS             16182..16358
                     /locus_tag="IVZ67_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8165244.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="IVZ67_001156"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="IVZ67_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8165245.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="IVZ67_001157"
     CDS             16853..17320
                     /locus_tag="IVZ67_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165246.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="IVZ67_001158"
     CDS             17405..18289
                     /locus_tag="IVZ67_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8165247.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18406..18639
                     /locus_tag="IVZ67_001159"
     CDS             18406..18639
                     /locus_tag="IVZ67_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8165248.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="IVZ67_001160"
     tRNA            18727..18807
                     /locus_tag="IVZ67_001160"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="IVZ67_001161"
     tRNA            18814..18885
                     /locus_tag="IVZ67_001161"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19401..20108
                     /locus_tag="IVZ67_001162"
     CDS             19401..20108
                     /locus_tag="IVZ67_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8165249.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="IVZ67_001163"
     CDS             complement(20167..20811)
                     /locus_tag="IVZ67_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8165250.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="IVZ67_001164"
     CDS             20938..21225
                     /locus_tag="IVZ67_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165251.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="IVZ67_001165"
     CDS             21333..21653
                     /locus_tag="IVZ67_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165252.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="IVZ67_001166"
     CDS             21971..23047
                     /locus_tag="IVZ67_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8165253.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23653
                     /locus_tag="IVZ67_001167"
                     /pseudo
     CDS             23044..23653
                     /locus_tag="IVZ67_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DACCZY010000036        23632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCZY010000036 DACCZY010000000
VERSION     DACCZY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.036"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            14..646
                     /locus_tag="IVZ67_001168"
     CDS             14..646
                     /locus_tag="IVZ67_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165254.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="IVZ67_001169"
     CDS             complement(780..905)
                     /locus_tag="IVZ67_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165255.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="IVZ67_001170"
     CDS             complement(1039..1932)
                     /locus_tag="IVZ67_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8165256.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="IVZ67_001171"
     CDS             complement(2114..3040)
                     /locus_tag="IVZ67_001171"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8165257.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVZ67_001172"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVZ67_001172"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8165258.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVZ67_001173"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVZ67_001173"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8165259.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVZ67_001174"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVZ67_001174"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8165260.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVZ67_001175"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVZ67_001175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8165261.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="IVZ67_001176"
     CDS             complement(11311..11745)
                     /locus_tag="IVZ67_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8165262.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="IVZ67_001177"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="IVZ67_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8165263.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="IVZ67_001178"
     CDS             complement(12642..13517)
                     /locus_tag="IVZ67_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8165264.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVZ67_001179"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVZ67_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8165265.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVZ67_001180"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVZ67_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8165266.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVZ67_001181"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVZ67_001181"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8165267.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVZ67_001182"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVZ67_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8165268.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVZ67_001183"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVZ67_001183"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8165269.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVZ67_001184"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVZ67_001184"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8165270.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVZ67_001185"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVZ67_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8165271.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="IVZ67_001186"
     CDS             complement(21968..22735)
                     /locus_tag="IVZ67_001186"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8165272.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVZ67_001187"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVZ67_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8165273.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6146 a         5107 c         4071 g         8308 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt ac
//
LOCUS       DACCZY010000037        22959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCZY010000037 DACCZY010000000
VERSION     DACCZY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.037"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            154..522
                     /locus_tag="IVZ67_001188"
     CDS             154..522
                     /locus_tag="IVZ67_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8165274.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            702..1643
                     /locus_tag="IVZ67_001189"
     CDS             702..1643
                     /locus_tag="IVZ67_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8165275.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1712..2443
                     /locus_tag="IVZ67_001190"
     CDS             1712..2443
                     /locus_tag="IVZ67_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8165276.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2587..4767
                     /locus_tag="IVZ67_001191"
     CDS             2587..4767
                     /locus_tag="IVZ67_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8165277.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4787..5269
                     /locus_tag="IVZ67_001192"
     CDS             4787..5269
                     /locus_tag="IVZ67_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8165278.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5285..6046
                     /locus_tag="IVZ67_001193"
     CDS             5285..6046
                     /locus_tag="IVZ67_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8165279.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6177..7511
                     /locus_tag="IVZ67_001194"
     CDS             6177..7511
                     /locus_tag="IVZ67_001194"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8165280.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7818..8303
                     /locus_tag="IVZ67_001195"
     CDS             7818..8303
                     /locus_tag="IVZ67_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8165281.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8296..8979
                     /locus_tag="IVZ67_001196"
     CDS             8296..8979
                     /locus_tag="IVZ67_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8165282.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9105..11750
                     /gene="polA"
                     /locus_tag="IVZ67_001197"
     CDS             9105..11750
                     /gene="polA"
                     /locus_tag="IVZ67_001197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8165283.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11795..12631
                     /gene="mutM"
                     /locus_tag="IVZ67_001198"
     CDS             11795..12631
                     /gene="mutM"
                     /locus_tag="IVZ67_001198"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8165284.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12595..13224
                     /locus_tag="IVZ67_001199"
     CDS             12595..13224
                     /locus_tag="IVZ67_001199"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8165285.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13276..13608
                     /locus_tag="IVZ67_001200"
     CDS             13276..13608
                     /locus_tag="IVZ67_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8165286.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13920..14411
                     /gene="nrdR"
                     /locus_tag="IVZ67_001201"
     CDS             13920..14411
                     /gene="nrdR"
                     /locus_tag="IVZ67_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8165287.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14434..15807
                     /locus_tag="IVZ67_001202"
     CDS             14434..15807
                     /locus_tag="IVZ67_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8165288.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15807..16733
                     /gene="dnaI"
                     /locus_tag="IVZ67_001203"
     CDS             15807..16733
                     /gene="dnaI"
                     /locus_tag="IVZ67_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8165289.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16862..17302
                     /locus_tag="IVZ67_001204"
     CDS             16862..17302
                     /locus_tag="IVZ67_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8165290.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17446..18147
                     /locus_tag="IVZ67_001205"
     CDS             17446..18147
                     /locus_tag="IVZ67_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8165291.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18144..19265
                     /locus_tag="IVZ67_001206"
     CDS             18144..19265
                     /locus_tag="IVZ67_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8165292.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19280..20512
                     /gene="pepT"
                     /locus_tag="IVZ67_001207"
     CDS             19280..20512
                     /gene="pepT"
                     /locus_tag="IVZ67_001207"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8165293.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20644..21552
                     /locus_tag="IVZ67_001208"
     CDS             20644..21552
                     /locus_tag="IVZ67_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8165294.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21587..21859
                     /locus_tag="IVZ67_001209"
     CDS             21587..21859
                     /locus_tag="IVZ67_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8165295.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21870..22916
                     /locus_tag="IVZ67_001210"
     CDS             21870..22916
                     /locus_tag="IVZ67_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8165296.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7747 a         4003 c         5035 g         6174 t
ORIGIN      
        1 cgcaaataac aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac
       61 gattctgttg agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga
      121 gaatgttaga acagatcgtt taggaggaaa cagatgttta cgaatgaaat aaaaatcatg
      181 ctttatgtgg acgatgtaga aaaaaatgca acgttttgga gagcagttgg ctttgcggaa
      241 aaagatcgtc aagaaatgga tgggacgctt ataattgaag taggagttga ggacagtcaa
      301 acctcatttc tgttatatga tcgagcattt gttgagtccc attcacctgg cacagctact
      361 agtgccccat ctttgatgtt ttacagtaaa gatatctttg gactatataa aaaaatgcag
      421 acgcaatcag tcacattagg tgatatggta cagcttggag aagaatatgt ctttaacttt
      481 gctgatcccg atggcaacta ctttgcggtt acaggtaaat aatagaaata aagactttgt
      541 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
      601 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      661 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      721 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      781 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      841 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      901 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      961 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
     1021 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
     1081 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
     1141 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
     1201 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
     1261 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
     1321 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
     1381 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
     1441 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
     1501 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1561 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1621 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1681 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1741 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1801 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1861 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1921 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1981 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     2041 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     2101 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     2161 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     2221 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     2281 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     2341 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     2401 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     2461 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     2521 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2581 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2641 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2701 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2761 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2821 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2881 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2941 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     3001 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     3061 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     3121 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     3181 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     3241 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     3301 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     3361 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     3421 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     3481 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3541 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3601 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3661 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3721 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3781 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3841 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3901 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3961 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     4021 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     4081 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     4141 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     4201 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     4261 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     4321 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     4381 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     4441 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     4501 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4561 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4621 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4681 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4741 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4801 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4861 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4921 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4981 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     5041 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     5101 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     5161 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     5221 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     5281 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     5341 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     5401 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     5461 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     5521 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5581 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5641 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5701 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5761 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5821 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5881 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5941 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     6001 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     6061 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     6121 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     6181 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     6241 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     6301 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     6361 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     6421 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     6481 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6541 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6601 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6661 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6721 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6781 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6841 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6901 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6961 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     7021 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     7081 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     7141 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     7201 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     7261 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     7321 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     7381 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     7441 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     7501 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7561 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7621 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7681 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7741 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7801 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7861 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7921 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7981 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     8041 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     8101 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     8161 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     8221 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     8281 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     8341 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     8401 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     8461 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     8521 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8581 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8641 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8701 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8761 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8821 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8881 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8941 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     9001 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     9061 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     9121 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     9181 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     9241 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     9301 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     9361 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     9421 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     9481 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9541 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9601 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9661 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9721 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9781 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9841 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9901 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9961 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
    10021 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
    10081 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
    10141 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
    10201 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
    10261 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
    10321 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
    10381 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
    10441 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
    10501 tgagccaaga actggcagaa aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10561 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10621 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10681 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10741 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10801 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10861 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10921 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10981 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    11041 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    11101 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    11161 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    11221 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    11281 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    11341 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    11401 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    11461 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    11521 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11581 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11641 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11701 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11761 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11821 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11881 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11941 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    12001 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    12061 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    12121 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    12181 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    12241 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    12301 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    12361 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    12421 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    12481 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12541 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12601 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12661 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12721 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12781 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12841 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12901 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12961 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    13021 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    13081 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    13141 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    13201 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    13261 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    13321 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    13381 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    13441 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    13501 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc
    13561 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13621 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag
    13681 atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa
    13741 aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct
    13801 tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca
    13861 actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa
    13921 tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg
    13981 atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat
    14041 ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt
    14101 tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta
    14161 tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa
    14221 acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg
    14281 aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct
    14341 taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag
    14401 aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa
    14461 ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc
    14521 ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg
    14581 ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt
    14641 agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc
    14701 ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag
    14761 ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt
    14821 ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg
    14881 catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat
    14941 gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa
    15001 tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat
    15061 tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg
    15121 tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat
    15181 gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag
    15241 agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc
    15301 tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt
    15361 ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa
    15421 gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat
    15481 gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta
    15541 aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag
    15601 cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat
    15661 caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa
    15721 gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac
    15781 ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca
    15841 tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg
    15901 cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa
    15961 aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc
    16021 caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg
    16081 tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc
    16141 gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac
    16201 gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg
    16261 ctgatccaaa agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa
    16321 catatttatt aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag
    16381 tccattttcc gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag
    16441 aaaagttaga tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg
    16501 cgatgagtag ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg
    16561 aacagttacc aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt
    16621 ccgtcactca acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac
    16681 gttatttgac aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg
    16741 acaagtccaa ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac
    16801 agaaatgacc ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt
    16861 tatgagggct attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg
    16921 cggtagcgca gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa
    16981 aacaagcagt acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac
    17041 tcaagtagaa gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga
    17101 agatccagcg aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc
    17161 tgaaaaaata agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt
    17221 agttgagatg ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat
    17281 tgatgtttca ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt
    17341 atagaacgaa aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct
    17401 tattttattt ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat
    17461 ttatctcagc gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac
    17521 attggatcag atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat
    17581 gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa
    17641 gatggattag aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca
    17701 caagatggta ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc
    17761 ctagaagctg gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca
    17821 aacatcggag agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca
    17881 gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa
    17941 aaaccagtta cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa
    18001 agtgagatct tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa
    18061 cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa
    18121 atgattgagg aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg
    18181 aaagctattg tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa
    18241 cattggataa gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg
    18301 aagaagcaat caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc
    18361 ctgtaaaacg gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac
    18421 atgatattgc cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg
    18481 actggttttg tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga
    18541 ttcattataa aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag
    18601 tattagctgc agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga
    18661 ttggacacgg tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga
    18721 agacagaaca agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac
    18781 aagtgattca agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg
    18841 ctatagatga accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa
    18901 tttctataga ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa
    18961 tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg
    19021 gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata
    19081 tctattatca tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc
    19141 attatattga atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag
    19201 aagaaaattg ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta
    19261 attaaaagga gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa
    19321 aacagaaaca cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc
    19381 ttttgcgcaa gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa
    19441 tgaagataac ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc
    19501 cattggattt atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca
    19561 aatcattgaa aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac
    19621 attgaataca aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac
    19681 agatggcagt acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc
    19741 aatggagatc ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg
    19801 tcctgatgaa gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga
    19861 ttttgcttat acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc
    19921 tgctcaagca aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac
    19981 aatgatcaat gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt
    20041 tccagaaaaa acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc
    20101 tgaagaagct tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg
    20161 caaagcacaa atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat
    20221 caaagtagat atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag
    20281 catcgtggaa ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc
    20341 agttcgtgga ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct
    20401 atttgccgga ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga
    20461 aaaagcaaca gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa
    20521 aagaacagct tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa
    20581 agggcaggac aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa
    20641 agaatggaaa agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa
    20701 ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta
    20761 gctgaaactg gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa
    20821 gctgatactt tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc
    20881 caaaaaacat tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt
    20941 gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc
    21001 aatgtacgga aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca
    21061 atcggaatct tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc
    21121 tcgtggatct ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg
    21181 gatttagaaa cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg
    21241 tctaactccg tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa
    21301 tttcatgtaa cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga
    21361 atcatgagag gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg
    21421 aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat
    21481 cttccagttg aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa
    21541 gaaaaagctt agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt
    21601 acacaatcct ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg
    21661 gtatgataaa catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt
    21721 tgtcagcctg ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa
    21781 aactcggaaa aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct
    21841 catttatttt ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca
    21901 ttctgaatct gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat
    21961 catttgcaga tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg
    22021 ttatattagg gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac
    22081 ctttcgcgcc gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt
    22141 accgaatcgt ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa
    22201 aaggaatgaa gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac
    22261 tggcagtgat cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta
    22321 atggtccatc aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg
    22381 cctttttatt ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga
    22441 ataagttatt taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct
    22501 actctttaag ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt
    22561 atctatggaa tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg
    22621 aaaaccatcc taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc
    22681 tttcaggggt tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat
    22741 tattcagacc agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca
    22801 gttcctttat cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag
    22861 tgatcaaaga agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct
    22921 tttataagat ctaccctttt tatcaaatca aagaaaaac
//
LOCUS       DACCZY010000038        22751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCZY010000038 DACCZY010000000
VERSION     DACCZY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.038"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..499)
                     /locus_tag="IVZ67_001211"
     CDS             complement(122..499)
                     /locus_tag="IVZ67_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165297.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="IVZ67_001212"
     CDS             755..1483
                     /locus_tag="IVZ67_001212"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8165298.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="IVZ67_001213"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="IVZ67_001213"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8165299.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="IVZ67_001214"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="IVZ67_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8165300.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="IVZ67_001215"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="IVZ67_001215"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8165301.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="IVZ67_001216"
     CDS             4618..6057
                     /locus_tag="IVZ67_001216"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8165302.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6089..7132
                     /locus_tag="IVZ67_001217"
     CDS             6089..7132
                     /locus_tag="IVZ67_001217"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8165303.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="IVZ67_001218"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="IVZ67_001218"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8165304.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="IVZ67_001219"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="IVZ67_001219"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8165305.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="IVZ67_001220"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="IVZ67_001220"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8165306.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="IVZ67_001221"
     CDS             10651..11565
                     /locus_tag="IVZ67_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8165307.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="IVZ67_001222"
     CDS             complement(11698..13263)
                     /locus_tag="IVZ67_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8165308.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="IVZ67_001223"
     CDS             complement(13256..14707)
                     /locus_tag="IVZ67_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8165309.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="IVZ67_001224"
     CDS             complement(14721..15737)
                     /locus_tag="IVZ67_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8165310.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="IVZ67_001225"
     CDS             15957..17045
                     /locus_tag="IVZ67_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8165311.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="IVZ67_001226"
     CDS             17099..18625
                     /locus_tag="IVZ67_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8165312.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="IVZ67_001227"
     CDS             18627..19832
                     /locus_tag="IVZ67_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8165313.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="IVZ67_001228"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="IVZ67_001228"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8165314.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..>22751
                     /locus_tag="IVZ67_001229"
     CDS             21330..>22751
                     /locus_tag="IVZ67_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8165315.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7322 a         3839 c         5155 g         6435 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga a
//
LOCUS       DACCZY010000039        22495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCZY010000039 DACCZY010000000
VERSION     DACCZY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.039"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..124)
                     /locus_tag="IVZ67_001230"
     CDS             complement(<1..124)
                     /locus_tag="IVZ67_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HAP8165316.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKL"
     gene            complement(215..667)
                     /locus_tag="IVZ67_001231"
     CDS             complement(215..667)
                     /locus_tag="IVZ67_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8165317.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(680..1090)
                     /locus_tag="IVZ67_001232"
     CDS             complement(680..1090)
                     /locus_tag="IVZ67_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165318.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1365..1577
                     /locus_tag="IVZ67_001233"
     CDS             1365..1577
                     /locus_tag="IVZ67_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165319.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1580..1792
                     /locus_tag="IVZ67_001234"
     CDS             1580..1792
                     /locus_tag="IVZ67_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165320.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2084..2308)
                     /locus_tag="IVZ67_001235"
     CDS             complement(2084..2308)
                     /locus_tag="IVZ67_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8165321.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2386..2574
                     /locus_tag="IVZ67_001236"
     CDS             2386..2574
                     /locus_tag="IVZ67_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165322.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2900..3235
                     /locus_tag="IVZ67_001237"
     CDS             2900..3235
                     /locus_tag="IVZ67_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165323.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3468..4409
                     /locus_tag="IVZ67_001238"
     CDS             3468..4409
                     /locus_tag="IVZ67_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8165324.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4411..5301
                     /locus_tag="IVZ67_001239"
     CDS             4411..5301
                     /locus_tag="IVZ67_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP8165325.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5344..6144
                     /locus_tag="IVZ67_001240"
     CDS             5344..6144
                     /locus_tag="IVZ67_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8165326.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6159..7010
                     /locus_tag="IVZ67_001241"
     CDS             6159..7010
                     /locus_tag="IVZ67_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8165327.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7211..7672
                     /locus_tag="IVZ67_001242"
     CDS             7211..7672
                     /locus_tag="IVZ67_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8165328.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7698..8150
                     /locus_tag="IVZ67_001243"
     CDS             7698..8150
                     /locus_tag="IVZ67_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165329.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8256..8450
                     /locus_tag="IVZ67_001244"
     CDS             8256..8450
                     /locus_tag="IVZ67_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165330.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8481..8867
                     /locus_tag="IVZ67_001245"
     CDS             8481..8867
                     /locus_tag="IVZ67_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165331.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8864..9067
                     /locus_tag="IVZ67_001246"
     CDS             8864..9067
                     /locus_tag="IVZ67_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8165332.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9064..9360
                     /locus_tag="IVZ67_001247"
     CDS             9064..9360
                     /locus_tag="IVZ67_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165333.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9436..9852
                     /locus_tag="IVZ67_001248"
     CDS             9436..9852
                     /locus_tag="IVZ67_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8165334.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10660..10890
                     /locus_tag="IVZ67_001249"
     CDS             10660..10890
                     /locus_tag="IVZ67_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165335.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11049..11882
                     /locus_tag="IVZ67_001250"
     CDS             11049..11882
                     /locus_tag="IVZ67_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP8165336.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11875..13290
                     /locus_tag="IVZ67_001251"
     CDS             11875..13290
                     /locus_tag="IVZ67_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP8165337.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13302..14831
                     /locus_tag="IVZ67_001252"
     CDS             13302..14831
                     /locus_tag="IVZ67_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8165338.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14923..15801
                     /locus_tag="IVZ67_001253"
     CDS             14923..15801
                     /locus_tag="IVZ67_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8165339.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15803..16120
                     /locus_tag="IVZ67_001254"
     CDS             15803..16120
                     /locus_tag="IVZ67_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165340.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16169..16870
                     /locus_tag="IVZ67_001255"
     CDS             16169..16870
                     /locus_tag="IVZ67_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8165341.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16885..17775
                     /locus_tag="IVZ67_001256"
     CDS             16885..17775
                     /locus_tag="IVZ67_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP8165342.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17798..18013
                     /locus_tag="IVZ67_001257"
     CDS             17798..18013
                     /locus_tag="IVZ67_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165343.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18025..18366
                     /locus_tag="IVZ67_001258"
     CDS             18025..18366
                     /locus_tag="IVZ67_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165344.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18366..18737
                     /locus_tag="IVZ67_001259"
     CDS             18366..18737
                     /locus_tag="IVZ67_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165345.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18730..19125
                     /locus_tag="IVZ67_001260"
     CDS             18730..19125
                     /locus_tag="IVZ67_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8165346.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19127..19504
                     /locus_tag="IVZ67_001261"
     CDS             19127..19504
                     /locus_tag="IVZ67_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165347.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19505..20113
                     /locus_tag="IVZ67_001262"
     CDS             19505..20113
                     /locus_tag="IVZ67_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8165348.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20113..20454
                     /locus_tag="IVZ67_001263"
     CDS             20113..20454
                     /locus_tag="IVZ67_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165349.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20696..>22495
                     /locus_tag="IVZ67_001264"
     CDS             20696..>22495
                     /locus_tag="IVZ67_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP8165350.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAI"
BASE COUNT         8132 a         3283 c         4646 g         6434 t
ORIGIN      
        1 gtaatttatt tagtttttca tcattttttt gttctaattc ataagcttct ttttttattg
       61 tgttagcttt cgacagttta gtgattccta taattaataa aataagtcca atcaataata
      121 acattcaaaa ctcctcattt ctttgatata attattgtag agtttactca gaaatgagga
      181 aagagtccat gtgaaagcat gggctttttt atttttaatg ttgtaatact aaatgttcaa
      241 gattatgagg aagccctaaa tattcaataa tttggaactg actcatatca gataaatctt
      301 gttcatctat tgttctagtt aacatttcaa ttgcaaacat attagcttca gcttctatac
      361 cataaatcat acaactaagg ttgtttgctc taaaagctgc tgtgcaaata cctttatgga
      421 gtctaacgtg ggctatttca tgccataata caaatctttt catagaaggt gataggtttt
      481 gattaattaa tatcacataa catcttttat tgtgtatcgt ttgtcctaat gttttattcc
      541 ctaaatcagt ttcaattaaa tctatatcag aatatttgat tattttatat ggattaatcg
      601 catcagatcc aaactctaca aataattttt ctatttcttc taatatccag tatttgttta
      661 aatgcatacc aaagcccacc tattgacgat attttttagg agtgaatttt ttctttgctt
      721 taactttggt taaggcaagt gcaagttgaa tggcatcgta taatttttcc ttatcttcat
      781 cgtctaaagg ttcaccataa taatttgttt cagcatctga attcaagcct tcaagtaatt
      841 tttcagcttg taaagcaata tcttgtttat ctttttctgt taaatcataa taatggcgtt
      901 tatcagtacg tcctaaaaga taatcagttg atacattaaa gtaatctgca actttttgta
      961 ttttatctcc agatggagta cttttattcc atctagacat gcttccattt ccgaaattta
     1021 actttctttc aagttctgcc aacgtcattg aacgttcttt taataagatt tttatacggt
     1081 ctaatagcat aggatatgca ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa
     1141 attatctatt tacaaataga gaatttgcag ttatactagt tttgtaagca agatatttac
     1201 ggcatacaaa gactaaaagc aataaactaa tagtctcccc agacatcagt aagctcgctt
     1261 tttgtgcgct gtattttgta tgcttacata atagcatatt tgcagttttt gtcaaactta
     1321 ttagacaaaa atattagaaa atattcgata agggggtatg aagcatgact ttaaaacagc
     1381 aagtattaat tgctttagta aagaaaggtt ggagtcaaag agagttagct cgacgaatgg
     1441 gtatatcaat tacttattta cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt
     1501 taaaacaaat cgaagaactt ttggatatca agttagaagt agattctaat gatcagccat
     1561 cagaacagga ggcgtaaaaa tggtacaacg tttgagcaaa gcggagttta aagagaaata
     1621 taagtattct gaaagtactt atcaaagaag aatgaaggaa tttaaaaaat cacgttttag
     1681 tgaggggtat aacgcagtta cttccaaaga aataacaatt gatgtcgatt tatatgaaca
     1741 gtttagaaca tggcgttcta aaaatcgatt tagagttaga gctgtttgtt aatagctaaa
     1801 aaatctaaat tcaccacttc tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa
     1861 gtccacgttt tttcattttg gcaactcctc ttcttggagg tggtgacggc gagagacgaa
     1921 ggagggatga catgaaggaa gaatcaaaaa tagggatttt gaatgagaac aattacacaa
     1981 gattaattat tgaaacagaa gaagggaaaa aagtagcaga agttacattg accgatgcaa
     2041 ctcctgctga cggttatcgt attcgattaa ctcctaaata tgattaacct tttggtgggt
     2101 gtggatcgtt tccgtgacta tcacggcttt gaatttttcc atctctgcca tgaataaata
     2161 gctcagatcc ttgattttta gaaatctctc tagcgatttt tactgcttct gattttgtat
     2221 tagtatgtgc agtcgcttta gaatttccag cacctttgac attccaacca ccattggaat
     2281 taggtacaac atgttgattt tttcccatag ttttcacctc catttctata gaaatatttt
     2341 atcaaataga aatggagaaa ttcaataaac gaaaggagaa gtgatatggc atataccttg
     2401 caacaagaac atcaaattct cggtttgatt aaacaacgca ggaaacaatt acaagatgac
     2461 cgtgcagcgc ttagaaaagc cgatgagcta tcagataggc aagctgaact aattgcttct
     2521 gaacttgagg atttgagaat gctagaaata aaaaataggg agattagatt atgaagaaga
     2581 cagacacact ttttatagga ttcattttgg ggttattagt gattgtagcg caccaaagta
     2641 ttatcggggg aagcttgttc gcagcattga tggttttaat caatctgctt gattcaaaag
     2701 aaaggagcaa ctatggcacg agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat
     2761 cggtggggaa atagccgccc tattatttta agcaagcaac atatcgaaaa gcaaaaagca
     2821 tggcaacaaa taaaaaagtg actctgccag ccagcaaaga gtcacataca aatttataaa
     2881 actaggagaa ttatatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg
     2941 tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa
     3001 aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca
     3061 tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta
     3121 cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa
     3181 caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat
     3241 gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt
     3301 tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc
     3361 agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga
     3421 ttatgctaaa ccaccatatg aagcaccaat tgatgaggag gatgaagatg agtaaatcta
     3481 ccttagaaat gagccatcaa gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg
     3541 atgttggaac aattttagga ttgaataaat ggaaatctcc ttatcaacta tggctagaaa
     3601 aaacaggaca agtagtactt gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt
     3661 tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa
     3721 accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag
     3781 tgggagaaaa tgccattctg gaatgcaaaa cagctaatca atttctcggt aaagaatggg
     3841 aaggcgaaga ggtaccgctt agctatctct gtcaagttca acattacatg aacgttctaa
     3901 acaaagacta ttgttacatc gctgtcttaa tcggtggtca aaaatttatc tggaagcgga
     3961 ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa
     4021 caaacgttct tggaggtatc gagccaatta ttgatggtag tcaagcgaca gctgatttct
     4081 tgaaagagaa gtatgcagat gttgaagacg ttcaaacgac gttaccaatt catttcgatg
     4141 aactagttga acagaaaaac gaactcaaac ggactaagaa agaaattgaa tcagctatcc
     4201 gtcaagtaga caacgagatt atcagcgagc taggtaaacg tgaagccagt atcggtatca
     4261 ctcaaaaaaa catcatcagt tggaaatttg tccgtacgag acgtatgaac tcgaagaaac
     4321 tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa
     4381 gaaggctaac cgaaagggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa
     4441 ttggcagcaa agccacagaa acaagttgca ccaggtcaat taggacttaa agctttgatg
     4501 aatacaccaa caatgagaaa gaagttcgaa gaggtgcttc atgacaatgc taatgctttt
     4561 atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg
     4621 tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta
     4681 ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag
     4741 aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa
     4801 tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg
     4861 acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt
     4921 ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc
     4981 gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg
     5041 gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg
     5101 ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt
     5161 caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa
     5221 gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct
     5281 aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata
     5341 cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc
     5401 aatccttata tgtataaatt atggaacttg tgtttgatga aagcgagtca tgagaatcgc
     5461 aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc
     5521 gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt
     5581 tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact
     5641 acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa
     5701 atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat
     5761 tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac
     5821 gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat
     5881 caggaaatca aaaagccgaa tctgcaaagt tgggcagata acattcggaa aatgatcgag
     5941 atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg
     6001 ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca
     6061 gcgcaagcca atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt
     6121 agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca
     6181 tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc
     6241 ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta
     6301 tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca
     6361 acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc
     6421 aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag
     6481 cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg
     6541 ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag
     6601 tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg
     6661 atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga
     6721 ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa
     6781 aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac
     6841 gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt
     6901 acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt
     6961 ctcaaacagc agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa
     7021 acaagaaaaa agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga
     7081 cgaattatct caacaagaga gtcgaatggt tcggaagata agtaagctaa ttatttatcg
     7141 ggatacagaa agatttctca ctgttttagg taacgtctac gagaatccag agttattgga
     7201 ggtagagtaa ttgccaaaaa tattagatgc atgttgcggt agtagattgt tctggtttga
     7261 taagaacaat tctgacgtaa catttatgga ttgtcgtcag cagtatgaag aattatcaac
     7321 aggtcatgtg atcaatgttg atccagatgt ggtcgcagac tttcgcgata tgccttttga
     7381 agatgatgtg ttcgatatgg ttgtatttga cccgccacac ttaagacatg tcggcgaaaa
     7441 ctcatggctt gccaaaaaat atggaaaatt agacgaactt tggccagaag acatcaggca
     7501 aggttttgca gagtgtatgc gagttttacg accttcagga acattaattt ttaagtggaa
     7561 cgaggaacag ataccacttt ctgatgtgtt agaggctatt ggagaacagc cattgtttgg
     7621 taataaacga agcaaaacac attggcttgt gtttatgaaa aaattcagct aacggaggaa
     7681 acaaaggagg aaaagtaatg aagattgtat taaataaatg ttttggtggc tttgggttga
     7741 gccatgaagc aaaaatggaa atttttaaaa gaaaaaatat tgaagtattc ccatacatca
     7801 ataactttag ctatgactca gatgatgaat acacgagata cacaggccaa aagctaggtt
     7861 cgatggattt tatctactat ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt
     7921 atagcgaaat tgaccgactg tacggaattg ctgatgattc cagtttttca agtgattcaa
     7981 acagaggtga taaggacctc gttgctgttg tcgaaaaact aggaagtgag gccagtggac
     8041 catatgcaag tcttaaagtc gttgatatac ccgatggggc tgagtgggaa ataagtgatt
     8101 atgacggcgt tgaaactgca cattatggtt ttcaaacagg tagtatttag tccactaatg
     8161 acaactaaag aggaggcaga aggatgagtg aagatgaata ctttgaacag tagtctatta
     8221 acgacagaga tttttaactg gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata
     8281 aaattaatag tctcaaagag gttttcggag acgtcgaagt agtgataaac gcacaaggct
     8341 atgacaacgg attttttaag cagattaccg acattagcat tcagcaagga atggaagacg
     8401 aagatggaaa ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca
     8461 gctaaagagg aggcagaagg atgaaaaaat tgcaccaact catatctgag aaagaaagcg
     8521 aactccaaaa tttagaagat agtttgggat tggggtttcc aattgttgag caagctaaaa
     8581 tgactcaaat cagtcatcta cgacttgaat tggaagattt aagacaaata gaaaaatcaa
     8641 ttcaactcaa cgacaaccag aaaatcgtgt tggagtggtt gaaaagcgag acaattttaa
     8701 ctagagaagc accaatatta tctgttaatg ctttttctga taaaaattta ctaggaaaat
     8761 tacctgataa agtacgcaaa gcttataaac tattggcttg taaacaagaa tatgaagtcc
     8821 tatcagcatt cgctcaatgg ggattagaac aggaggaagc ggaatgaaca acagacatcg
     8881 cagaataaca aaactaagaa aacaggaact gaatgtacta aagacaaagt ttgaaaaaga
     8941 atatggaatt tcagcagaag aaacatataa agtggcaagt cagtttgttg ctgatgcgag
     9001 taatgctatt cgtaagtttg ggatttcgat attaaatgat gatcgtaaat gggaggaaat
     9061 gagatgaaaa taaaagacgg attttacgct agtagtcatg gtatcggcgg tttaatgcta
     9121 gacatgccga taaagaaccc taaaacacgt cagaaatcaa aattcaaaat cggtgacatg
     9181 gtccgatgta aagcagaaga gttcatctat ccgtttcgtg gatatgtaga gcatgtctat
     9241 aatcattcag caatcattca cattgaaaac acgatggaat gcgataagtg gttagcgaaa
     9301 agcaaagata atttagcagt ggctcgtttg gtggatatgg aactaatcaa tgacaaataa
     9361 aaaagccgga tcgctccgac tgattcaata aatccaacac atttattata tcacataaag
     9421 gagcggtttg acttgatgca attgttacga gaggtagatt tcaaacagac aagatgtaat
     9481 gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga tggcaggtcg ctctttgata
     9541 gatattaagt cgccgattat tacggatatg ccgaaggcac cgaagcacgg caataaggca
     9601 gaagacgcga tcattcagat gatggatata gaagcggaga gagacgcaat actagcggct
     9661 ttgatggctc ttagtctgat tagccgtcag atactctact acagcttctg tgacgtaaac
     9721 aagcactcta attatgaaat agggcaattg atacgaggat acggagagaa gaatgtagag
     9781 aagttgaaat ccatcgcatt aatcgaattt gcagaagcat acaaaaaagg cgtgttagtt
     9841 cagtatcgtt gattttgtag ggtttttgta gggatagtgt agggtttttg agcggtttaa
     9901 cgtgatatta tggtagtgtc gaaagattag tgataggtct gagacaaaat aaaatgtaag
     9961 ggaggaaatc tccctcatcg ttttaactta agcttcgata gacagcaacg gaaatattaa
    10021 gaataaggat gtgaatttca actccttcta aattgttctt attatctatc atccgttgtt
    10081 gtctattaat ttatgcatcg gaggaaaaac gaatggataa agaaatcaaa gtaactgtca
    10141 aattagattt gactgaacta aaagaactgc tcaacaaggc tagtgaccaa gtcgaacagt
    10201 tacaagaaac tttagatgaa attgctaatt ttaaaatcca agtttcttag cagtatattt
    10261 tttagcagct gcactcataa aatcatacca agtatcagaa aaatataact taactaaata
    10321 attaaaaaag gtgggtaaat agaaatgacg gcgttaataa tttcaatttt tgcgctctgt
    10381 cttaatgtct atatgattgg atttaaaaat ggacaaaata aaaaatagta gcagccgaga
    10441 ataattttat agtgtcactg tggcggaaat agaagacgca aaggtggagg taggaagcgt
    10501 atggcgcact atcgggacct tgtacaaaac tagaaaggac tagtacgtgt gtggtgcgag
    10561 ccctagggag gaacaggata accgcctgtg tgtaggttgc tattacatat tagatcactc
    10621 tttgagtggt ctttttattt acataaagga ggctacataa tgagaaacta ctggtatata
    10681 tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct
    10741 gtccaaatca aaaagaagta ctccattgtt gaaatgacca gagaagctac gccaaaagag
    10801 attgataaat gcaaacttct ttattgcggt catggtttct atgatgaacc aaacattcaa
    10861 aataatatta acaagaattt gagggattag aatgcaagaa atggcttaaa aatctccaat
    10921 caggacaaat caagttagac aaagtttccg atttaaagat attgattgaa gctgatttaa
    10981 tgctaaaaga tattgaaaat tagaaaacaa aactcaacct aagaagattg cgaggtggtg
    11041 tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga atttggttag
    11101 aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt tcagattctc
    11161 aggttagaaa atggaaatcg caagacaaat ggagcgctga attgaaaagt aacgttacca
    11221 atggcaaaag taacgttact aatcaaggtg gcgctcctat tggtaatcaa aatgctaaag
    11281 gtaacaaagg aaacagcaga gcatcaccgc caaagagaaa taagaatgct cttaaaactg
    11341 gtgagtatga aacaatattt tttgacacgt taaccgataa tgagaaggac atctattcta
    11401 gtttgaatga tgatccttct tttgctttgt ccgaagaaat acgacttctt aaaataagac
    11461 aacttcgaat gatgaaaaga atccaacaag ctgaagctgg actaaatgat gaagaagtcg
    11521 aacgattgca gcagctaaga aagattaaaa ctccgattga aaaaaatggt aaaaagctag
    11581 aaatcaagcg tgaggttatg caagatgtgc agattagcag aaaaaaacat cgcaaaattg
    11641 atgatattct ttcaattgaa gattcattga ctcggattag caaccagtta gctaaagcca
    11701 tcaagcaaat gaatgaactt tatatgaatg aatacagaac tgatttaatt aaagctcaga
    11761 ctgataagat ccaagctgag acaaatgaaa ttggcggaaa taattcaggt gaagaaatag
    11821 aagaatggaa acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa gaaaatgaat
    11881 aatgaattta ttccttttgc tgatattggt tctgccattg attattacta tgataaacca
    11941 gtagcttttt gccaagatat tttgcatttg aatcctgatg aatggcaaga aaatgtttta
    12001 aatgatttag ctgaattttc aaaggtttct gttcgttctg gtcaaggagt tggaaaaaca
    12061 gcattagaag caggagcaat actttggttc ttaacgtgtc gaccctacgc taaagtaata
    12121 gcaacagctc cgacaatgaa gcaactttac gatgtacttt gggcagaggt agctaaatgg
    12181 ttaaatgata gcttgatcaa aaacttactg aagtggacaa agaccaaaat ttatatggtt
    12241 ggtgattcag agcgttggtt tgctacggct agaacagcga ctaaaccaga aaatatgcaa
    12301 ggttttcacg aggaccatat gttgattgtg gtagatgaag cttctggtgt gtctgatcca
    12361 attatggaag ctattcttgg tacgctatca ggttttgata ataagctgtt gatgtgtgga
    12421 aaccctaata atattgaagg tgtcttttac gattcccaca attcagaccg tgataaatac
    12481 agagttcata aagtatcaag ctatgatagt aaacgtacaa acaaagacaa tatagaaatg
    12541 attcttaaaa aatatggaaa agaaagtgat gttgctcgtg tccgtatttt tggagaattt
    12601 cccaaaggtg cgttggattc atttatcagt cttgaaacgg ttgaattggc tacagaaaaa
    12661 caaattagtg attctttagt caataaaaca acggttgctc atattggtgt tgacgtagct
    12721 cgatatggtg atgattctac gattctcttt cctagaattg ctaccagggc attggagtat
    12781 gagaagtatt caaaacgtag cactatggaa acaacaggat atgtcatcaa catggccaag
    12841 aatctaatga gtcaatatcc gagtattgat aaagtgatga ttaaagtcga tgacactggt
    12901 gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag aagacaagca ttatcctttt
    12961 gaggtgtttg gagtgaataa cggttcaaca tcagaagacg atttttacga taatttaggt
    13021 actcaactat ggggaaacat caaggaaatg ttagaagaaa atatgacagc aaatcttaac
    13081 ggagaacagc ctgttattga attgccttct gatagttcgt taatcaaaga attaagtact
    13141 cgcaaattca aaatgacaag tagaagtcgt atacgtttag aaagtaaaga tgatatgaaa
    13201 aagcgaaata ttggtagtcc cgatattgct gacgcactgg ctttagcgtt ttatgagcca
    13261 ccaagtcact atcaatttat tcaattttag gaggtgagtt tttgattagt acagtattat
    13321 ctttagagag atataagaag ctacgtgtta aatatgcaac acaaattgaa gatggaatgt
    13381 ttgatccgaa tggttttata gaatatatga aaccattttt tgctgatcgc gaaagaaaat
    13441 atctagctta tactagcgaa aagaatgaaa tagacaatag accaaagcct aacactgata
    13501 ttgtaaaagt taataataaa cttcatgctg gtatgtattc aattgtcgta gaccaagcag
    13561 tcaatcattt tactggtata cctatcaaat gggattacga tgtatcggaa caaaaaagaa
    13621 cactcattca aagattaaaa gataaattct taaaaaatga tatagaactt ccaacagttc
    13681 cagaagcttt taacaaatta acaagcaatc ttgattccat gagattcgca atgcttgatt
    13741 ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt ccgattgtta gagcctgtgg
    13801 aagaagatga tggttggaag ttaagagcaa gtaatattga accttggaga gcagaaagat
    13861 atggaaatgc cggaatctat atcaaagaaa agtatgactc ttaccagaaa aaattttttc
    13921 aagaaatgaa agttatgaca agaaataaaa ttctgacata tgcttgctat ggtgatttga
    13981 attttgttac aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct ttggggacga
    14041 ttaccttggc agaatttaaa aataatacga atcgttattg tgattttgaa gtagctgagg
    14101 aaattggaga tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta
    14161 agcttgcgta catgcttgtt actggcacaa caatgagcaa aggcactgct aaagaaatga
    14221 tgaatcagct agggattatc aacttaaaag accctactgc aaaagctgaa tatgtcacaa
    14281 aaaaattagc aaaagatttc aatgaatatc atatggatct attgaaaaaa cagttttaca
    14341 ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa atcaaacagt tctggagaag
    14401 ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa tactaaagag caatatttta
    14461 gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt catgacaaag
    14521 tagaaattga gcctgaaaaa attattttta ctttctctag atctttgcca actgatttaa
    14581 gttatttagc tgaagcttta cctaaacttg caccatacgt atccaaacga actattcaga
    14641 gccaaattcc atttgtaaca gatgttgatt atgaaaatga aatgatggaa ttggaaagtg
    14701 gaagtgctta tccagatagt gaatacaatt ttggcggagg tggcaatagt gacgatagaa
    14761 acgaagtatt ggaccaaacg tcgagaactg gaggatcaag caaggctcaa acaagaaaat
    14821 cagacactta aaaaattaac tagtgtattt cctgaagcac tgaaagagat acaagcaaaa
    14881 ctattatcac aagctgactt acacaatatc acttatccag aaatgatgga gttttacagt
    14941 acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa attataagtc attaaaaatg
    15001 tatgatgcaa aatacaaaga gtttatcgat gaattttttc caccatttga ctatgcaaaa
    15061 gtcaatcgct tattacaaat acgatcagat gtatttaaaa ttcttgcaga atatgcgatg
    15121 gatgcagatg tgaatcaata tttttctgat cgcttagagg aaattcttca aagaacatat
    15181 tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg atataccaaa ttatttacca
    15241 gaaaatgaaa ttaaacatta tttgaactat ccatggtcag gaaagacatt ttcaagaaga
    15301 ctttggggaa atatttcatc gcttgaacag aaactctcta atgctattgt aaaaagtgtt
    15361 gctagtggag aaggcgttat acacgcatta aacaccatga gattagactc agaaatttgt
    15421 gacatgttta agttagaaga atcaaagtat aacaaagcga tagaaaatct cgttcgaacg
    15481 gagtatgcaa aatttgcaca agatggtatt gaaaaatcat atttagaaac aggtattgag
    15541 gaatacaacg tattgactgc aaaagatgag agagtttgcc ggatttgtgg aggaaaggca
    15601 agtaagaatc cctataaact gaaagatgct gtcataggtg aaaatcgagc acctttccat
    15661 agtcgttgta gatgtacgga tgttcctaat ttaccaaaat taggaaagga tattgatgaa
    15721 gaatatgacc gtttatttgg cgatttatta gatgagtttg cacatgattc ttttggaatt
    15781 aatttgaaac ggaggaagta gaatgaaaga tttttttgaa gcagtactaa caattaatgt
    15841 aaatgctgat attgcagaag cctacaaaac agctattgag tctgagaacc atcctaatgg
    15901 cttgagagac cattggaatg gcaattatgc ctacgtggtt attggcgatc aaactgttaa
    15961 ttatcaagat aatactccag ttgataagaa taccgttaat ttaacgattc aattattatc
    16021 tcattcatta ccaaatttaa aagaaacagt tgattggtat gaaaagatgg gatgtattgt
    16081 tgttagaact gactacaaag aaggaaagtc tagtaattag gcttttttat tttgtccgaa
    16141 atgacgttaa actagcgcaa tactgggctt ggttgaatgg tggggcgcaa ctattaaaac
    16201 tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca
    16261 caaatcatta atgccaatga atttgcaata ctttgccgaa ggtgacgatc aaaagttttc
    16321 ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc
    16381 acaatctcaa agtgttgccg acaaacaatt ggaagcttgg aaacaaaata acttagataa
    16441 aattaaacag gataccatca aggaatatga ggaatctaag aaaaacaagt cacctgagca
    16501 aattcaactg gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag
    16561 tgataataaa gcatttgttg cagaacaaat tgctggatta gaactagatg gagagttaaa
    16621 agagtctatt tctcaattta tgctaaataa tcttgttagt tcggatacag atttcactaa
    16681 gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt aatgagaaac atgcagatgc
    16741 attaaaagaa ctacaaatga agtctgcatt tggtggaact caacaatcga ataaccaggt
    16801 tcagcagaac aatgaaacat taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca
    16861 atttaactag gagagtgaaa aattatgaaa aaaacatctt taaataattt agagtatttg
    16921 gatatttcac cagcgattaa tgctatgcaa gtaccaaata cacctttttt aagctattta
    16981 cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat
    17041 atcaacaacg atgattcttc tgaaaaactt gagggcggag aatatccaga tgctgaatca
    17101 ggtcgaactt ggtttaacaa ctatactgaa atttttagaa aatcaacctc tgtatctggt
    17161 acattagatg ctattaatgt gaatggagtc ggaaatgaat taactaatca agtagcacta
    17221 cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga ttactggtgt aaaagctgat
    17281 gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga acttgatcaa ttcagcaaat
    17341 aaggcagaaa cagccactgc gggtgcagta acaagaaaag atatcgatac tttatttaaa
    17401 ttgatgtatg aaaaaggtta tatgggagaa aaactatgct tgatttctcc agatatgcag
    17461 gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga
    17521 gtaacttttg gattgcaaat tggaaatatc gtgtctaatt acggtacagg tattgctcta
    17581 ctagaaccat cattgccaaa aggaacaatt gccgcaatcg atactaatta tgtgaaacta
    17641 cgtccattac gtgaatggag agcagaagaa cttgcaaaaa caacggattc tagacgtatc
    17701 ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa actctggggc aattttaaat
    17761 ttaaagtctg aataaaaggg agttagtact cccttttttg ataggaggaa ttacagtggt
    17821 aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat agagtaggta aaactaaaaa
    17881 ttttgttggg tttgttcatc ctaaaactcg tagatttatc acagcagatt caaataacga
    17941 atttatcatt tctatagatg ataaagaagc aattgcaatt ttggaagatg caattgatgt
    18001 taatgaaatt taggaagtga tctgatggat gaatcgctaa aaacggaaat cattgagtct
    18061 acaaaagaag attttccaga tttgagtgaa gaacgcataa ctaatttatt agaaataatt
    18121 ttgctagaaa ttgaatcata caacacttgt aaaaatgata tttcatggga aaagttaaaa
    18181 agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa aacagtatct
    18241 tcagttagac gtggcgatac aacgattagt tatgcttcaa caacaaatga tgttagtgag
    18301 ttacttctag gctatggtga tttgatacga agagttattg gttgtggagg attggagttt
    18361 ttttaatgaa tgaagcagat attttagaaa ctacttatga agatagttgt attattgaaa
    18421 gactgacgga cattgaagat tctaatacaa atattactat tcaagattac aaaaaagtat
    18481 atgataatcc tatttcttgc gctctttcac aaggtcaaat cgatggacta gcagtcatag
    18541 aagatggaga gatggtaaat gtttcaactg acacatataa attatttgtt catcctaaga
    18601 ttaaactaaa aaaaggagat cgaataacaa taactcaaaa ggccagtggc ttaatttttt
    18661 ctctatttgc tactaagcct ttttactatc ctagtcattg tgaagtgaat ttgataggaa
    18721 gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag aaaagatcat tgagaatttt
    18781 aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat
    18841 aaagtcgtta gtttagctaa accattaact cctgtaaaat caggtaattt aaggcgagga
    18901 tatcgtgtag taaaagctag aaaactatca agcggtcgaa ttgttggagc agttataaac
    18961 aatgaacatt attttaaata tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc
    19021 aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta tattcctcga
    19081 agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat
    19141 cattgctgca atcagtagca aattaaaaga aatctatcct gatgggacaa tatatcttga
    19201 ttcagttatg cagtcaacta aagattttta tttcgtttta tcagtaatgg aatctggaac
    19261 tgaaaatgta ggaattgata ttcaaaatgt ttctttctta attgatattg cattgattga
    19321 taataaacct aatagaaatt taattaatga attagtctca cgttgtggga ctttttttaa
    19381 tacgattaca atcgatgagc aaatattatt tccaaaagcc tatttacctg atgaagcaga
    19441 tggtgttcaa catattcgtt ttacattaga atttccacaa tatattgaat ggagtgaaag
    19501 ataaatggga gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt
    19561 tcctttggaa gatgagacag ggaattttcc ggtatatggt actgcgttgc caatgggaac
    19621 agcagtaagt atcaaaccaa cagctaatta tgaaacaaca caagattatg gggattcagt
    19681 ggttcaagat caatttacag cttttggtgg tgcagaagtt gaactggaag caaatggata
    19741 tactcataaa gttttatcta caattactgg agggaaaatt gttaaaggtg gtgccttgcg
    19801 ttctggagaa gatattgcgc aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg
    19861 gaaataccgt tatacggtct tttataaagg acagtttgct ctagattcag atgaaacatc
    19921 aacgattgaa ggaagtaaag taagttttac tcatccaact tggaagggtt catttgtaga
    19981 tgtaccagga cttggatata tgtattcagt tgatgaagat gatgaaggcg tggataaagc
    20041 aatgatcgga aattggttta caaaagtagc aattccaatt gaagaagcag aactttcagg
    20101 aggtacagaa taatgtcaaa atatcaaaca acgattaaat taacgaaaaa agatgaagaa
    20161 ggtaaatatg agcaagtaca atttaaatct gctgaatttc taccaggccc agtcgtagaa
    20221 gaagcagcag aagtaatgga gggaatgcaa aatgctgtag atggaaaatc agtatcagaa
    20281 gcattaagtc gagcttattc atttattgca gatattttgt ttgaaggaca gtttactggt
    20341 gaagattatc gtcaagggat cgatgctcga gaaattgctt cgttgacagg taagttatta
    20401 aaatctgtta ctgcaggctt tgatgaaact tatacagaaa cgaaaaaaaa gtaagcgaag
    20461 ctctcaaata tccttttttt aaacattcga ttacttatcg agaattagat ttaaagacgc
    20521 aattactcga agcaggttgg acgttaccag aaattgaacg tactgacttt gacgagttaa
    20581 tgcgtctttt tgcttttcga gatgcagtaa aagagcatga ggatgttgag tactacgata
    20641 acttcaccca attttaggag gtgatacttt gaataacgat gacctaattc tgaagatgat
    20701 actagatgaa tctggtttta ctgccggtat gaataatgct gtcaaaaagt tgaactcttt
    20761 tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt tcattaggta gtatttggaa
    20821 aatatttgct ggaagttttc tagctagcgg agtaactaga atcgtaggag ctggctttga
    20881 tctaatcaag ggttccataa gtggagcggt tgatcgagta gatacgatga ataacgctct
    20941 acgaaatttc caaaatatgg gatttagtaa ttcagaaatt atgaagaata tcggaaagaa
    21001 tgggctttta tctcaaggta ttcaaggact tcctactgct ttgaatgatg cgataagtca
    21061 tgttcaactt cttgcttctt ctacaggtga tttaactcgt tcaactcaaa tatttaaagc
    21121 tttaaatgat ggaattcttg gtttcggtgg ttcaactgat caagttaatg aagctgttat
    21181 tcaattatct caaagtttct caaacggaaa agtagacgca caaacttgga actcaatgat
    21241 caacgctcaa cttggaccta ctctttctgc tattgctaaa aagatgggaa ttacaatggg
    21301 agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt
    21361 aatagaaatg gataccaaag gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga
    21421 ttctactaag ggaattaaaa cctctataca aaatgcaaaa acagctgtta cacgtggtgt
    21481 tggtgaggta atagaaggat taaataaagc attagtagat tcagatttag gtggatttaa
    21541 gggaattatt gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa
    21601 tattcctaca gcagtatcat ttttaagtaa tttatttaac gaaattcaaa aatttggttc
    21661 tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct accgcttttg gagcgttcat
    21721 gtttcaactt aaaggtatac cagcaataat aaaaagcttc aataatttta agaatgccat
    21781 aattggcgtt ggaaattcat taaagattat gggggcaata gctgctgcta attcatttgt
    21841 tttaattatt ggtgcagtcg ttggtgcaat agctgttttc ggttacttta tggcaaccaa
    21901 tgaagagctt agaaacaagg ttatatctat ttggaatgat gtgaaagatt ccgtaattgg
    21961 tgtattaaaa aatataaagg actggggaat tgatacttgg aattctgcta aagaaatggc
    22021 atcaaatgca gttgagggtg tcaaagatgc ttggtcagga acaaaggaat ggttttctaa
    22081 cacctggaaa ggtattaagg atggggccac aggtttattt gataaaacag tagagacttc
    22141 aagaaatgca gtcgatagtg taaaaaatgc atggtctaat atgaagaaat ggttttctga
    22201 tacttggcaa agtataaaag attcagccag agaaaaatgg aatggaatca aaggttctat
    22261 tatgtcagtt gctggtccat tgattacagg tataaagaat gcatttttac atgttacttt
    22321 ttatctagag accttatgga ataatttagt tgagattggt aaaaacgtct ttgagatttt
    22381 aaaaaatgtt atacttgcgc cggttttgtt tattacctcc atgatttctg gtggatggga
    22441 agaaacaaaa aataatatga ttgctgtttg gaataatatt aaagaaagtg ccata
//
LOCUS       DACCZY010000040        22249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCZY010000040 DACCZY010000000
VERSION     DACCZY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.040"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(135..653)
                     /locus_tag="IVZ67_001265"
     CDS             complement(135..653)
                     /locus_tag="IVZ67_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8165351.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(655..1272)
                     /locus_tag="IVZ67_001266"
     CDS             complement(655..1272)
                     /locus_tag="IVZ67_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8165352.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1269..2123)
                     /locus_tag="IVZ67_001267"
     CDS             complement(1269..2123)
                     /locus_tag="IVZ67_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8165353.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2120..3031)
                     /locus_tag="IVZ67_001268"
     CDS             complement(2120..3031)
                     /locus_tag="IVZ67_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8165354.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3072..4406)
                     /locus_tag="IVZ67_001269"
     CDS             complement(3072..4406)
                     /locus_tag="IVZ67_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8165355.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4614..5444
                     /locus_tag="IVZ67_001270"
     CDS             4614..5444
                     /locus_tag="IVZ67_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8165356.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5688..5937)
                     /locus_tag="IVZ67_001271"
                     /pseudo
     CDS             complement(5688..5937)
                     /locus_tag="IVZ67_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6128..6322)
                     /locus_tag="IVZ67_001272"
     CDS             complement(6128..6322)
                     /locus_tag="IVZ67_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165357.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6827..7012)
                     /locus_tag="IVZ67_001273"
     CDS             complement(6827..7012)
                     /locus_tag="IVZ67_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165358.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7862..8203)
                     /locus_tag="IVZ67_001274"
     CDS             complement(7862..8203)
                     /locus_tag="IVZ67_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8165359.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8203..8433)
                     /locus_tag="IVZ67_001275"
     CDS             complement(8203..8433)
                     /locus_tag="IVZ67_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8165360.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8720..9058
                     /locus_tag="IVZ67_001276"
     CDS             8720..9058
                     /locus_tag="IVZ67_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165361.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9263..9502)
                     /locus_tag="IVZ67_001277"
     CDS             complement(9263..9502)
                     /locus_tag="IVZ67_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8165362.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9478..9825)
                     /locus_tag="IVZ67_001278"
     CDS             complement(9478..9825)
                     /locus_tag="IVZ67_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8165363.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10557..11138)
                     /locus_tag="IVZ67_001279"
     CDS             complement(10557..11138)
                     /locus_tag="IVZ67_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8165364.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11321..11947)
                     /locus_tag="IVZ67_001280"
     CDS             complement(11321..11947)
                     /locus_tag="IVZ67_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8165365.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11969..13300)
                     /locus_tag="IVZ67_001281"
     CDS             complement(11969..13300)
                     /locus_tag="IVZ67_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8165366.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13455..13856)
                     /locus_tag="IVZ67_001282"
     CDS             complement(13455..13856)
                     /locus_tag="IVZ67_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8165367.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13942..14148)
                     /locus_tag="IVZ67_001283"
     CDS             complement(13942..14148)
                     /locus_tag="IVZ67_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165368.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14243..15745)
                     /locus_tag="IVZ67_001284"
     CDS             complement(14243..15745)
                     /locus_tag="IVZ67_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8165369.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15763..16218)
                     /gene="argR"
                     /locus_tag="IVZ67_001285"
     CDS             complement(15763..16218)
                     /gene="argR"
                     /locus_tag="IVZ67_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8165370.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16316..16933)
                     /locus_tag="IVZ67_001286"
     CDS             complement(16316..16933)
                     /locus_tag="IVZ67_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165371.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17154..18527
                     /locus_tag="IVZ67_001287"
     CDS             17154..18527
                     /locus_tag="IVZ67_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8165372.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18602..19714
                     /locus_tag="IVZ67_001288"
     CDS             18602..19714
                     /locus_tag="IVZ67_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8165373.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19786..21423)
                     /locus_tag="IVZ67_001289"
     CDS             complement(19786..21423)
                     /locus_tag="IVZ67_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8165374.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21591..22199)
                     /locus_tag="IVZ67_001290"
     CDS             complement(21591..22199)
                     /locus_tag="IVZ67_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8165375.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6488 a         4536 c         3874 g         7351 t
ORIGIN      
        1 aaattttata acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac
       61 tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta
      121 ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact tcctaaaata
      181 gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc
      241 aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct
      301 actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt
      361 gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg
      421 ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat
      481 tctctagtat caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca
      541 ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc
      601 ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc
      661 ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa
      721 caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag
      781 aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa
      841 aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa
      901 gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc
      961 cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc
     1021 taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga
     1081 accgagcttt ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact
     1141 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
     1201 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
     1261 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
     1321 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
     1381 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
     1441 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
     1501 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
     1561 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
     1621 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
     1681 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
     1741 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
     1801 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
     1861 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
     1921 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
     1981 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
     2041 attcctatta ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg
     2101 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
     2161 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
     2221 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
     2281 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
     2341 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
     2401 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
     2461 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
     2521 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
     2581 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
     2641 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
     2701 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
     2761 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
     2821 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
     2881 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
     2941 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
     3001 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
     3061 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
     3121 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
     3181 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
     3241 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
     3301 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
     3361 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
     3421 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
     3481 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
     3541 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
     3601 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
     3661 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
     3721 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
     3781 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
     3841 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
     3901 aataactttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
     3961 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
     4021 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
     4081 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
     4141 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
     4201 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
     4261 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
     4321 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
     4381 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
     4441 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
     4501 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
     4561 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
     4621 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
     4681 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
     4741 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
     4801 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
     4861 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
     4921 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
     4981 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
     5041 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
     5101 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
     5161 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
     5221 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
     5281 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
     5341 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
     5401 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
     5461 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
     5521 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
     5581 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
     5641 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
     5701 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
     5761 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
     5821 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
     5881 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
     5941 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
     6001 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
     6061 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
     6121 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
     6181 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
     6241 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
     6301 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
     6361 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
     6421 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
     6481 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
     6541 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
     6601 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
     6661 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
     6721 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
     6781 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
     6841 taaaataacc aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa
     6901 taaatgttga aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat
     6961 accctataaa agctaataag atggttagga atcctttaga atttttactc attcatagac
     7021 acttccttaa ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg
     7081 actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa
     7141 aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg
     7201 taatcaatta gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt
     7261 gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt
     7321 ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt
     7381 tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga
     7441 tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc
     7501 ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga
     7561 gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat
     7621 tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa
     7681 aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta
     7741 gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg
     7801 gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg
     7861 cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt
     7921 ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt
     7981 gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg
     8041 aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga
     8101 ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata
     8161 caatatcacc ttttttagga atataaattc tttcttctga cactataaaa tttctctccc
     8221 ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact
     8281 aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat
     8341 atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga
     8401 acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata
     8461 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
     8521 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
     8581 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
     8641 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
     8701 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
     8761 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
     8821 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
     8881 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
     8941 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
     9001 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
     9061 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
     9121 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
     9181 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
     9241 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
     9301 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
     9361 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
     9421 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     9481 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     9541 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     9601 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     9661 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     9721 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     9781 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     9841 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     9901 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     9961 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
    10021 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
    10081 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
    10141 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
    10201 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
    10261 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
    10321 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
    10381 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
    10441 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
    10501 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
    10561 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
    10621 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
    10681 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
    10741 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
    10801 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
    10861 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
    10921 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
    10981 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
    11041 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
    11101 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
    11161 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
    11221 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
    11281 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
    11341 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
    11401 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
    11461 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
    11521 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
    11581 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
    11641 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
    11701 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
    11761 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
    11821 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
    11881 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
    11941 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
    12001 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
    12061 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
    12121 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
    12181 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
    12241 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
    12301 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
    12361 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
    12421 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
    12481 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
    12541 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
    12601 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
    12661 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
    12721 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
    12781 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
    12841 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
    12901 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
    12961 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
    13021 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
    13081 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
    13141 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
    13201 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
    13261 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
    13321 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
    13381 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
    13441 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
    13501 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
    13561 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
    13621 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
    13681 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
    13741 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
    13801 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
    13861 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
    13921 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
    13981 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
    14041 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
    14101 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
    14161 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
    14221 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
    14281 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
    14341 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
    14401 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
    14461 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
    14521 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
    14581 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
    14641 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
    14701 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
    14761 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
    14821 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
    14881 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
    14941 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
    15001 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
    15061 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
    15121 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
    15181 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
    15241 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
    15301 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
    15361 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
    15421 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
    15481 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
    15541 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
    15601 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
    15661 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
    15721 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
    15781 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
    15841 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
    15901 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
    15961 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
    16021 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
    16081 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
    16141 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
    16201 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
    16261 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
    16321 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
    16381 cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga
    16441 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
    16501 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
    16561 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
    16621 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
    16681 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
    16741 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
    16801 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
    16861 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
    16921 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
    16981 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
    17041 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
    17101 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
    17161 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
    17221 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
    17281 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
    17341 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
    17401 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
    17461 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
    17521 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
    17581 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
    17641 catggaatca tatcttcctg tttcttgtgc ccgttttgtt tctttctttg attatcggct
    17701 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
    17761 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
    17821 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
    17881 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
    17941 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
    18001 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
    18061 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
    18121 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
    18181 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
    18241 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
    18301 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
    18361 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
    18421 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    18481 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    18541 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    18601 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    18661 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    18721 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    18781 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    18841 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    18901 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    18961 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    19021 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    19081 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    19141 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    19201 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    19261 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    19321 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    19381 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    19441 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    19501 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    19561 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    19621 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    19681 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    19741 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    19801 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    19861 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    19921 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    19981 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    20041 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    20101 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    20161 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    20221 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    20281 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    20341 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    20401 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    20461 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    20521 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    20581 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    20641 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    20701 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    20761 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    20821 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    20881 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    20941 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    21001 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    21061 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    21121 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    21181 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    21241 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    21301 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    21361 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    21421 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    21481 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    21541 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    21601 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    21661 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    21721 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    21781 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    21841 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    21901 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    21961 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    22021 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    22081 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    22141 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    22201 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaactt
//
LOCUS       DACCZY010000041        22168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCZY010000041 DACCZY010000000
VERSION     DACCZY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.041"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            234..2069
                     /gene="tdc"
                     /locus_tag="IVZ67_001291"
     CDS             234..2069
                     /gene="tdc"
                     /locus_tag="IVZ67_001291"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8165376.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2374..3852
                     /locus_tag="IVZ67_001292"
     CDS             2374..3852
                     /locus_tag="IVZ67_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165377.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3818..5149
                     /locus_tag="IVZ67_001293"
     CDS             3818..5149
                     /locus_tag="IVZ67_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165378.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5263..7815
                     /locus_tag="IVZ67_001294"
     CDS             5263..7815
                     /locus_tag="IVZ67_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8165379.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7822..9261)
                     /locus_tag="IVZ67_001295"
     CDS             complement(7822..9261)
                     /locus_tag="IVZ67_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8165380.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9607..9969
                     /locus_tag="IVZ67_001296"
     CDS             9607..9969
                     /locus_tag="IVZ67_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165381.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10156..11013)
                     /locus_tag="IVZ67_001297"
     CDS             complement(10156..11013)
                     /locus_tag="IVZ67_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8165382.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11133..12044
                     /locus_tag="IVZ67_001298"
     CDS             11133..12044
                     /locus_tag="IVZ67_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8165383.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12051..13702
                     /gene="mdcA"
                     /locus_tag="IVZ67_001299"
                     /pseudo
     CDS             12051..13702
                     /gene="mdcA"
                     /locus_tag="IVZ67_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13804..14103
                     /locus_tag="IVZ67_001300"
     CDS             13804..14103
                     /locus_tag="IVZ67_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8165384.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14093..15769
                     /gene="mdcD"
                     /locus_tag="IVZ67_001301"
     CDS             14093..15769
                     /gene="mdcD"
                     /locus_tag="IVZ67_001301"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8165385.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15773..16366
                     /locus_tag="IVZ67_001302"
     CDS             15773..16366
                     /locus_tag="IVZ67_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8165386.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16390..17466
                     /locus_tag="IVZ67_001303"
     CDS             16390..17466
                     /locus_tag="IVZ67_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8165387.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17491..18360
                     /gene="mdcB"
                     /locus_tag="IVZ67_001304"
     CDS             17491..18360
                     /gene="mdcB"
                     /locus_tag="IVZ67_001304"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8165388.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18471..19235
                     /locus_tag="IVZ67_001305"
     CDS             18471..19235
                     /locus_tag="IVZ67_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8165389.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19724..21817
                     /locus_tag="IVZ67_001306"
     CDS             19724..21817
                     /locus_tag="IVZ67_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8165390.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21842..22144
                     /locus_tag="IVZ67_001307"
     CDS             21842..22144
                     /locus_tag="IVZ67_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165391.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7178 a         3788 c         4723 g         6479 t
ORIGIN      
        1 ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt gttcgctttt
       61 ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa aatctacgtg
      121 gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag
      181 ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag
      241 atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa
      301 tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat attccttcag
      361 atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc
      421 atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc
      481 attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag
      541 cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa
      601 cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg
      661 gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc
      721 gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa
      781 tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct
      841 ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc
      901 aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag
      961 atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa
     1021 tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc
     1081 taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa
     1141 tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg
     1201 ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt
     1261 atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg
     1321 acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc
     1381 ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata
     1441 tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag
     1501 atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct
     1561 ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt
     1621 caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag
     1681 gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat
     1741 tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg
     1801 atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta
     1861 catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa
     1921 cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc
     1981 cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg
     2041 aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag
     2101 acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt
     2161 catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt
     2221 ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt
     2281 ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa
     2341 aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa
     2401 atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt
     2461 ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg
     2521 tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt
     2581 ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg
     2641 ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg
     2701 tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc
     2761 attttagtcg tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc
     2821 ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta
     2881 gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca
     2941 tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc
     3001 atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca
     3061 gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt
     3121 ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc
     3181 gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg
     3241 tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca
     3301 gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg
     3361 aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca
     3421 atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta
     3481 aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga
     3541 ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag
     3601 agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc
     3661 ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc
     3721 attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa
     3781 gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg
     3841 gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt
     3901 atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt
     3961 gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa
     4021 tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt
     4081 ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc
     4141 aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag
     4201 cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga
     4261 atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg
     4321 attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat
     4381 cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt
     4441 cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa
     4501 gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac
     4561 gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt
     4621 agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat
     4681 tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata
     4741 tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt
     4801 acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt
     4861 ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt
     4921 tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg
     4981 gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt
     5041 cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt
     5101 attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg cccagataca
     5161 ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc
     5221 cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg
     5281 acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa
     5341 cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa
     5401 cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc
     5461 tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg
     5521 atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt
     5581 ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc
     5641 cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca
     5701 gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga
     5761 gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac
     5821 cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt
     5881 gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa
     5941 caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc
     6001 attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg
     6061 catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca
     6121 gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc
     6181 atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag
     6241 acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc
     6301 tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca
     6361 gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat
     6421 ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca
     6481 acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta
     6541 tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa
     6601 tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag
     6661 gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat
     6721 ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg
     6781 aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta
     6841 aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg
     6901 attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga
     6961 attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat
     7021 gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa
     7081 gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa
     7141 gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg
     7201 ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta
     7261 cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat
     7321 gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag
     7381 caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg
     7441 gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt
     7501 ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag
     7561 ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt
     7621 gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta
     7681 agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt
     7741 gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga
     7801 gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt
     7861 gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag
     7921 agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag
     7981 ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa
     8041 cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat
     8101 agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt
     8161 gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc
     8221 atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa
     8281 tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt
     8341 ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc
     8401 taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt
     8461 ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc
     8521 ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt
     8581 atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca
     8641 acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa
     8701 aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt
     8761 gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca
     8821 agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt
     8881 agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac
     8941 agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa
     9001 aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc
     9061 ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga
     9121 aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt
     9181 atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat
     9241 ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat
     9301 aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac
     9361 tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca aaaaataggt
     9421 gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac aggcggattg
     9481 aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca actacttgta
     9541 ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag aaagagatgg
     9601 aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata caatttgtct
     9661 ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca aaaaagcaaa
     9721 aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga tgcaagtgac
     9781 cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg aattttgcga
     9841 ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat ctctattgat
     9901 ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag ttttaaatca
     9961 acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt agcttcaaga
    10021 aataagaagg aatttacgaa aattgcttct caaatttttg ggaaatttcg gcttattttc
    10081 taagaaatta cttctggaac accgtttgtt cgaaaaaaga gctgcgacaa gaattttgtc
    10141 ccagctcttt tttttttatt ttcctaaata aaattcccgc atcgtttttg caatcatttt
    10201 gatcaaagga tgatcttctg tgaagctaga agataaataa attgttcgtg tcaagggcaa
    10261 atctttgatt tcccgcaaga cgaaaggatc atttttatcg taagaccaag aaacttttgg
    10321 gaaaaagcta acagctaatc cttgcttgac aatagaacgt aatgtcgcag gatcatctgt
    10381 cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt gctgcatcca gtgtatgacg
    10441 gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt tggatatctt ctaattgcag
    10501 atgttctttt ttagcaagag gatgtttctt aggaatggcg acataaacag gttctttcat
    10561 gataggaata ttggtcatgc caggcttcat ctccgaagtg atgtacagta caggatcggt
    10621 aatatcatct ggtaaatgat gttgcatgat cgttaattgg atatcaggat gcttttccat
    10681 aaaaattcgg accaagttcg gaatagaaac agaggctacc tctaaataca atatgacgga
    10741 agtaacgatc ttctcattga ccgaagacaa taatttgatt ccttcttcta atgtgtctaa
    10801 tgtcttgact gcatatggat aaaattcttc tgcataaacg gtcgtctcaa tgtttctgcc
    10861 gctatggtaa aacaaaggga cacctaattc gttttccaaa tgtttgatcg cacggctcaa
    10921 ggcaggttga gaaataaata attctttggc tgcttgggta acattttttg tttcgaatac
    10981 ttttttaaaa tactgcagat gaagcaattt cataattatc catcccatcc ataccttttt
    11041 gttatggttt tttctttttt tggcattttt tattatagca gaaattgact aagatagcag
    11101 tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac aggtttctta tttcctggac
    11161 aaggcagtca gaagttaggg atgttagcgg atatccctga aatgtacatg gaacgtttag
    11221 aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa ctatcaagat acggttttta
    11281 tccagctagc attactgaca aaagcagtat tttatttaga tgaaatggaa aaaaacggta
    11341 ttcagcctga tattgcagca ggacattcga ttggtgcttt ttgtgcagct gttgcttgtc
    11401 gatctttgag atttgagaat gcagcggaac tagtctatca ccgcgcagtc ttaatgaaag
    11461 aagcttatcc aagcggttat gcaatgggcg tagtagtagg tgtgacgaga agcgaagctg
    11521 aggaaattgt agcaaaaaca tttgatcctg accatccagt ttatctgtca aatgaaaatt
    11581 gtccgatgca acatacgtta tctgggagta tcgaaggttt atccgttacg ttggcagaag
    11641 ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt ccctgtgcca tctcactgtc
    11701 tcttgatgaa tgatacggtc aagaaattca agccgtatat gcagtctgtc catctatctg
    11761 atgctagatg tttgtatata aaaaatacgg atggaagagc cactcaggac gcagaagaga
    11821 ttcgtaaaga tttgttggaa aacctagctt tccctgtgca gtggaaccaa atgatggact
    11881 tggcaaaaga attaggaatg gatctgacga ttgaatttcc ccccggcaat actttgacaa
    11941 agctgattca tacaaaattt ggtgtagata aagggatccg tacgatcaat ctagaccaac
    12001 atggtattga tgacgccacg tttctatatc aaaaatggag atgactaatt atggaaaaac
    12061 caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca gcgattggaa aaaatcagca
    12121 acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt agaagtaatg gaaaaactga
    12181 ttgctcccgg cgacaaagtt gtattagaag gcgacaatca aaaacaagca agttttcttt
    12241 cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga tctgcatttg atcatgtcta
    12301 gtatctcacg accagaacat ttgaatattt ttgaaaaagg aatcgcaagc aagatcgatt
    12361 tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat gattgaagac ggcacaatga
    12421 aattaggtga tatccatact tatttggaac tttatggacg cttattcatc gatttgattc
    12481 caaatgttgt tttagtagct gcagataaag cagataaaga cggcaacttg tacacaggtt
    12541 tcaatacgga agaaacacca acaatcatcg aagctgcagc tttcaaagat ggtatcgtga
    12601 tcgttcaagt taacgaagtt gtcgattctt taccacgtgt agatatccct ggtgactggg
    12661 tagacgttgt cgtgaaagca gataaacctt atcaattaga agctttattc acacgagatc
    12721 ctcaaaacat cacagaattg caaattctaa tggcaatgat ggcaatcaaa ggaatctacg
    12781 cagaacacgg cgtacaatct ttgaatcacg gtattggatt caatacagca gctatcgaat
    12841 tgttactgcc aacttatggt gaatcactag ggttaaaagg gaaaatcgcg aagaactggg
    12901 tattgaaccc acatccaacg atgattccag ccatcgaatc aggttgggtt gaaagcatcc
    12961 atagttttgg tggcgaagtt ggtatggaaa aatatattgc tgctcgccca gatgtctttt
    13021 ttgtcggtaa agacggcacg atgcgcagta accgaacatt atcacaagct gcaggacaat
    13081 atgccatcga tatgtttatc ggttcaacct tgcaattaga ttacgaagga aactcttcta
    13141 ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc taacatggga cataatcctg
    13201 gcggtcgtcg tcattctaca ccagcatggc aatcacttcg cgacaatgat gatccattag
    13261 ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata tggttcaaat aaaaaaccag
    13321 tctttgtcga agaattagat gcactagctg ttcaaaaaca agcaggctta gccaatgcaa
    13381 tgcaccagtg atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc
    13441 ttatttgtac aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg
    13501 tgttacacct cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg
    13561 catcgtgaaa ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt
    13621 agctgcacaa accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat
    13681 gaaattcaga acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga
    13741 tggaagaaga aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga
    13801 tccatggaac aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat
    13861 gtaggagtgg tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa
    13921 tctactgtaa aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta
    13981 acgagatttt ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct
    14041 acaccaggag ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa
    14101 tagttttgta gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga
    14161 attgattgat ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc
    14221 tgaaagtgat gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt
    14281 aatcgctatg gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat
    14341 cgttgctgca cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt
    14401 cattgtacta gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat
    14461 ttcagaaatc ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat
    14521 tccaggacgt gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt
    14581 aatcgcaaca aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc
    14641 aggcgttcga gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg
    14701 tcaacgggta gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa
    14761 agctgctgtc atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc
    14821 attttattta tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta
    14881 caatgaagca attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg
    14941 aaatgaacca gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa
    15001 aaatccaact tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga
    15061 agccatcgtg acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg
    15121 tgaagttggt ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga
    15181 agataaagac aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc
    15241 atacggctat aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc
    15301 ttatgcaaaa ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat
    15361 ttcaggtgca tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa
    15421 agaaattacc gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt
    15481 tgctgaaatc gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta
    15541 tacaaaacta ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga
    15601 acaaaatgtc agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac
    15661 aaatgacacg atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt
    15721 cgaaacaaat aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca
    15781 agcacatgat attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg
    15841 ggtcgttcta actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga
    15901 aatactaccg attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc
    15961 aaaacatgcc gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag
    16021 acaagagaaa atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt
    16081 atcaggatgt aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc
    16141 ggcagtaaaa gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt
    16201 gccaattcag gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt
    16261 gactcggcaa ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct
    16321 attgaaaaca gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga
    16381 gagaaagaga tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta
    16441 tcagctgtaa cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac
    16501 agcgcaacaa atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca
    16561 atgtttattt ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg
    16621 gaatttttga aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct
    16681 tatctttggc tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt
    16741 ttactgaaat atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg
    16801 cagatccaat tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa
    16861 aaatatgtat ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc
    16921 tatcaaacac aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt
    16981 gttgctagtt tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa
    17041 tatttggaca gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct
    17101 gtccgttcat tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat
    17161 acgatccaaa gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa
    17221 atgattgtaa acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat
    17281 cgtacgtatt tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt
    17341 tggctgcagt taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt
    17401 gcatttgctt taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc
    17461 aagtaatagt aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg
    17521 actcagcaac tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca
    17581 aaacctggat tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta
    17641 tttcttaagt ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg
    17701 catcatgcta ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa
    17761 aaggcaatgc tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg
    17821 gggctattga tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg
    17881 aaaatacttg atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa
    17941 aaaaccactc atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa
    18001 gcgatattag ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat
    18061 gattcatatg tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac
    18121 acatgcgtat tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat
    18181 gctgctagtc aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag
    18241 gaaaaccaaa tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg
    18301 aagatagagg aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa
    18361 ataagacaaa attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc
    18421 aaggctatca taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa
    18481 tcaattattt agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag
    18541 gattcgctgt cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag
    18601 gttcatttgg tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag
    18661 attcttatgg gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc
    18721 ttgcattagc tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa
    18781 gacaaaagaa aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa
    18841 agaaagcaca gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag
    18901 aagaaactcc tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga
    18961 aagttctgca aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc
    19021 aggaagtagg aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca
    19081 gatgctgtga ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac
    19141 aacgacagta tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc
    19201 aagccgattt tattcagccg attatgctgg attgacagga aatatttatg aacggagttg
    19261 tgacaaaagc cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat
    19321 cggcaataag gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc
    19381 gctcaaggct gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat
    19441 ctgcgataat aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct
    19501 tattacttga tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt
    19561 ttgtaataat aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt
    19621 ttgtttgctt tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat
    19681 gtaagcggaa ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat
    19741 tgaaatactt cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag
    19801 tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt
    19861 gagcagtaaa aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct
    19921 ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt
    19981 gaatcggcgc gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat
    20041 ccatctaaac aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat
    20101 gagacggtta cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca
    20161 aggactggtt tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa
    20221 tcagaatctg ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca
    20281 aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc
    20341 caataagttg ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt
    20401 gatctggatc cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac
    20461 agaaataaaa gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt
    20521 ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc
    20581 tagagatatc tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat
    20641 ccaatttgta gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga
    20701 aaatctctat aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat
    20761 gaatccattg aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca
    20821 ttttgttcct acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt
    20881 tcttgtcatc cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta
    20941 taaatcagtc gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct
    21001 gaaagaaaag tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt
    21061 agtagataaa ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa
    21121 gccgctttta gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt
    21181 tttgaaaaga aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt
    21241 attcactcaa ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta
    21301 tggcaagtta gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca
    21361 gcctatgtta aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac
    21421 ttgggaggaa tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga
    21481 gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg
    21541 caaacacatg gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag
    21601 cgtagctacg attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat
    21661 tattttttgt ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat
    21721 tgaattgatc aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga
    21781 tttcaaacaa ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa
    21841 gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc
    21901 acagatgtct gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg
    21961 tgatatttct tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg
    22021 ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat
    22081 tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat
    22141 ctaaatacaa aaagaaaaga gggtaaaa
//
LOCUS       DACCZY010000042        22061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCZY010000042 DACCZY010000000
VERSION     DACCZY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.042"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..1957)
                     /locus_tag="IVZ67_001308"
     CDS             complement(26..1957)
                     /locus_tag="IVZ67_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8165392.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVZ67_001309"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVZ67_001309"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8165393.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="IVZ67_001310"
     CDS             complement(2886..3638)
                     /locus_tag="IVZ67_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8165394.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="IVZ67_001311"
     CDS             complement(3797..4006)
                     /locus_tag="IVZ67_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165395.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="IVZ67_001312"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="IVZ67_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5709..6296)
                     /locus_tag="IVZ67_001313"
     CDS             complement(5709..6296)
                     /locus_tag="IVZ67_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8165396.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVZ67_001314"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVZ67_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8165397.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /locus_tag="IVZ67_001315"
     CDS             complement(7876..9165)
                     /locus_tag="IVZ67_001315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8165398.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="IVZ67_001316"
     CDS             complement(9319..10251)
                     /locus_tag="IVZ67_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165399.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="IVZ67_001317"
     CDS             complement(10408..11067)
                     /locus_tag="IVZ67_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8165400.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVZ67_001318"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVZ67_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8165401.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="IVZ67_001319"
     CDS             complement(12108..12398)
                     /locus_tag="IVZ67_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165402.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12807..14612
                     /gene="glmS"
                     /locus_tag="IVZ67_001320"
     CDS             12807..14612
                     /gene="glmS"
                     /locus_tag="IVZ67_001320"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8165403.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14684..16039)
                     /locus_tag="IVZ67_001321"
     CDS             complement(14684..16039)
                     /locus_tag="IVZ67_001321"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8165404.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16063..17232)
                     /locus_tag="IVZ67_001322"
     CDS             complement(16063..17232)
                     /locus_tag="IVZ67_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165405.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17229..18116)
                     /locus_tag="IVZ67_001323"
     CDS             complement(17229..18116)
                     /locus_tag="IVZ67_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8165406.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVZ67_001324"
     CDS             complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVZ67_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8165407.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         6079 a         4829 c         3916 g         7237 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    12541 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    12601 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    12661 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    12721 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    12781 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    12841 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    12901 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    12961 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    13021 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    13081 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    13141 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    13201 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    13261 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    13321 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    13381 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    13441 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    13501 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    13561 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    13621 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    13681 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    13741 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    13801 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    13861 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    13921 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    13981 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    14041 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    14101 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    14161 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    14221 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    14281 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    14341 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    14401 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    14461 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    14521 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    14581 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    14641 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    14701 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    14761 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    14821 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    14881 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    14941 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    15001 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    15061 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    15121 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    15181 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    15241 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    15301 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    15361 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    15421 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    15481 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    15541 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    15601 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    15661 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    15721 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    15781 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    15841 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    15901 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    15961 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    16021 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    16081 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    16141 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    16201 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    16261 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    16321 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    16381 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    16441 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    16501 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    16561 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    16621 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    16681 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    16741 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    16801 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    16861 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    16921 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    16981 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    17041 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    17101 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    17161 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    17221 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    17281 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    17341 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    17401 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    17461 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    17521 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    17581 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    17641 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    17701 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    17761 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    17821 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    17881 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    17941 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    18001 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    18061 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    18121 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    18181 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    18241 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    18301 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    18361 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    18421 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    18481 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    18541 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    18601 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    18661 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    18721 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    18781 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    18841 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    18901 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    18961 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    19021 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    19081 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    19141 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    19201 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    19261 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    19321 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    19381 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    19441 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    19501 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    19561 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    19621 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    19681 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    19741 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    19801 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    19861 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    19921 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    19981 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    20041 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    20101 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    20161 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    20221 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    20281 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    20341 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    20401 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    20461 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    20521 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    20581 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    20641 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    20701 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    20761 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    20821 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    20881 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    20941 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    21001 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    21061 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    21121 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    21181 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    21241 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    21301 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    21361 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    21421 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    21481 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    21541 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    21601 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    21661 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    21721 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    21781 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    21841 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    21901 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    21961 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    22021 ctctcttatt atctgttcca ttataacgta aaacatgcca t
//
LOCUS       DACCZY010000043        21831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCZY010000043 DACCZY010000000
VERSION     DACCZY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.043"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(548..2044)
                     /gene="lysS"
                     /locus_tag="IVZ67_001325"
     CDS             complement(548..2044)
                     /gene="lysS"
                     /locus_tag="IVZ67_001325"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8165408.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2155..3159)
                     /gene="dusB"
                     /locus_tag="IVZ67_001326"
     CDS             complement(2155..3159)
                     /gene="dusB"
                     /locus_tag="IVZ67_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8165409.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3160..4047)
                     /gene="hslO"
                     /locus_tag="IVZ67_001327"
     CDS             complement(3160..4047)
                     /gene="hslO"
                     /locus_tag="IVZ67_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8165410.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4264..6375)
                     /gene="hflB"
                     /locus_tag="IVZ67_001328"
     CDS             complement(4264..6375)
                     /gene="hflB"
                     /locus_tag="IVZ67_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8165411.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6465..7010)
                     /gene="hpt"
                     /locus_tag="IVZ67_001329"
     CDS             complement(6465..7010)
                     /gene="hpt"
                     /locus_tag="IVZ67_001329"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8165412.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7010..8455)
                     /gene="tilS"
                     /locus_tag="IVZ67_001330"
     CDS             complement(7010..8455)
                     /gene="tilS"
                     /locus_tag="IVZ67_001330"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8165413.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8575..9045)
                     /locus_tag="IVZ67_001331"
     CDS             complement(8575..9045)
                     /locus_tag="IVZ67_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8165414.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9091..9516)
                     /locus_tag="IVZ67_001332"
     CDS             complement(9091..9516)
                     /locus_tag="IVZ67_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8165415.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9583..9852)
                     /locus_tag="IVZ67_001333"
     CDS             complement(9583..9852)
                     /locus_tag="IVZ67_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8165416.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9863..11458)
                     /locus_tag="IVZ67_001334"
     CDS             complement(9863..11458)
                     /locus_tag="IVZ67_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8165417.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11473..14994)
                     /gene="mfd"
                     /locus_tag="IVZ67_001335"
     CDS             complement(11473..14994)
                     /gene="mfd"
                     /locus_tag="IVZ67_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8165418.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15011..15571)
                     /locus_tag="IVZ67_001336"
     CDS             complement(15011..15571)
                     /locus_tag="IVZ67_001336"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8165419.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15924..16898
                     /locus_tag="IVZ67_001337"
     CDS             15924..16898
                     /locus_tag="IVZ67_001337"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8165420.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17068..18468
                     /locus_tag="IVZ67_001338"
     CDS             17068..18468
                     /locus_tag="IVZ67_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8165421.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18546..19268)
                     /locus_tag="IVZ67_001339"
     CDS             complement(18546..19268)
                     /locus_tag="IVZ67_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8165422.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19347..19853)
                     /locus_tag="IVZ67_001340"
     CDS             complement(19347..19853)
                     /locus_tag="IVZ67_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8165423.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19976..21775
                     /locus_tag="IVZ67_001341"
     CDS             19976..21775
                     /locus_tag="IVZ67_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8165424.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6059 a         4756 c         3914 g         7102 t
ORIGIN      
        1 aaaggtctac ttcatcgaga gtgtttcccg attcattttt ggctgccgta gcaactttta
       61 tatcatatca gagagacaag cgattgtcaa ctctttttta aaagtttttt tcgatttcgt
      121 ttttcgaaat ctttcggcaa ctcatttatc ataacttatc gtttgttatt tgtcaagaac
      181 ttttttgaag tttttttcga tagaatcaag cttcgcaagt ggttcttttt taacgactca
      241 gttatcttat catgtcgtta gtaacatgtc aataactttt ttgttatgtt tttgaataaa
      301 tattttcttc tgtcttttgg acaagtaata atataccaac taaatttagc ttggtcaacg
      361 aattttttgt attttttcaa gtttttttca ataaacgatt attcataact tttctttctt
      421 tatcgttcgt tttcgtcttc tttaatgtct agaatagtcg gcattagctt ttgaactttc
      481 gttattattt attcctttct acacagcaaa agctgcctaa ggaattcctt agacagcttt
      541 tattcgatta tctcattgtt gggaatagca aaacatcccg aattgattgt gcatcagtta
      601 gcaacattac taatctgtcg atcccgatgc ctagaccacc agttggcggc ataccgtatt
      661 ctaaggcttc caagaagtct tcgtctacac cgtgagcttc gtcatttcct agttcacgtt
      721 ctttttcttg cgcttcgaat ctttctcttt gatcaattgg atcgttcagt tctgtaaagg
      781 cattagcaaa ttcgcgcccc actatgaata gttcgaatcg atctgtgaat cgaggatctt
      841 ctggattttt cttcgctaaa ggcgaaactt cgactggatg tccgtataca aacgtcggtt
      901 gtgtcaatgt ttcttcaaca aatgtttcaa agaattcatt gataacatgt ccgacaccca
      961 tagcttcggt tacttctaca ttgtgttttt cggctaaagc taatgcttct tccaatgtca
     1021 tttcttgcca gaagtctaca ccagtttgtt ctttgattgc atctaccata tggatgcgtt
     1081 taaagtcact ttctaagtcg acagcttgtc catcatatgt gatttttgct gttcctagta
     1141 ctttttcagc tgcgttacgg ataatacctt ctgtcagatt cattacatca ttgaaatcag
     1201 tatatgctgt gtatgcttct aacatcgtaa attctggatt atgtgtagta tcaattcctt
     1261 cattacggaa aacgcggccg atttcataaa ctttttccat tccgccaaca attagtcgtt
     1321 ttaaatgaag ttctagcgca atacgcaaat ataaatcaat gtccagtgca ttatgatgcg
     1381 tgataaatgg gcgtgcggca gctccacctg cctcattgtg caatactggt gtttctactt
     1441 caatatagcc attgccatct aaatagcgac ggatttcgct gataatttgg cttcgtttca
     1501 taaatcggtc aaagctttcg cgattactga ttaaatccaa gtagcgttgt ctgtagcgtt
     1561 gttcaatatt cgttaagcca tggtatttat ctggcagtgg acgaagtgct tttgacaaaa
     1621 tcgtgatttc tgaagctttg atagaaactt cgcctgtgtc tgttttcatg atttgtccag
     1681 ttacaccaaa gaaatctcca aggtctgcat gtttgaatac ttcataggcc tcatcaccta
     1741 cttggtcttt tcttacataa atttgaattt ggccttcacg atcttgtaga tgcgcaaatc
     1801 cagctttccc ttttcctcgg cgagtcatca tacgaccagc tacactagcg gtcaatccca
     1861 tttctgctag ttcttctttt gttcttggat caaatagttc atgcaattct tctgagttgt
     1921 gcgtgcgttc aaatcgtttt ccaaatggat cgattccatc ttcacgcaat tgttccattt
     1981 tttgacgacg tacaagcatt tgatcattta aatcttctac ttgatgttgt tgttcctgtg
     2041 tcacaaaagt tcctccattc tcatgttaat ctctttccta taatgccaat gaaacacaaa
     2101 aaaagcaagc cgaacttgga atttcggctt gaaaattcac gaaaaactta gaaatcacaa
     2161 cgtttgtttc aatagtactt tttccgcttt ttctacaaat tgatccagca ggtcattgat
     2221 ttcttgctgt ttttctgtct gattgattgc tactttcact ttcgctgctc gaggaatccc
     2281 ttttaaataa taagccgcat gtgttctgaa ttctctacaa gctacttttt ctcctttgag
     2341 atctgctaag cgttgtaaat gaagtttagc tgtagcaatt ttttcggctg gtgttggttc
     2401 aggaatcagt tcccctgttt cgagataatg ctgtgtgcgg tgaatcatcc atggattgcc
     2461 cagcgctgca cgaccaatca tcacaccgtc tgcaccgaca tagtcaagca tccattttgc
     2521 atcttctggc gttttgacat ccccgttccc cataaatgga atcgtcaaat gttttttcac
     2581 ttctttcaat acttcccagt ccgcttttcc ttcatacatc tgaacccgtg tacgtccatg
     2641 catagcgatg gcacttgctc cagctgattg tgctgctaat gcattttcta cagcaaagac
     2701 gtgctcttca tcccaaccaa tacgcatctt gattgttact gggatattta ctgctgaaga
     2761 aacagcttgg accatttcgt atactttctc aggatctaaa agccattttg ctcctgcttc
     2821 tgctttgatt actttattta ctggacagcc catattgata tctattatat cggcagctgt
     2881 gttttcttcg acaaattgag cagcttcaac tagactatct ttatttccgc caaatatctg
     2941 taaacttaat ggatgttctg tttcttcaat gtggagcatc tctaaagttt ttttgtttct
     3001 gaaatgaata ccttggtcac tgatcatttc acatacgact aagcccgcac caaattcttt
     3061 gacagtgact cgaaatgctg aatttgttat tccagccatc ggtgcaacaa cgacacgatt
     3121 agggatttcc acattgccaa ttttccatgt gtgttccact taaatccctc ctaaggcttc
     3181 tttcttcaat ccatctaatt cttcttcgct atacttgtat ttgttattac agaaagaaca
     3241 aaccgcttct gcaccgtgat cttcgtctat cattgcttgg atttcttctg cacctaacgc
     3301 gataattgct tctgcaaatt tttcttttga acagtcacat ttgaattgaa caggcatttt
     3361 ttccaaaatg ttcaattctt ctgtaccaag caatcctttc aatatttctt ctggtgtctg
     3421 tccttcatct aagagggtag agattcttgg tgtcgttttt aattgttctt cgatcttgct
     3481 gattgtttct tcatcggcac ctggcatgat ttggatcatg aatcctcccg ccgtcttgat
     3541 gctgtcgtca gtattgacca atacactcag tccaacagct gaagggattt gttctgagat
     3601 agcaagatag taagtaaaat cttcagcgat ttctccagaa acaatcggcg tttgcccaga
     3661 aaaaggttct tttaatccta agtcttttgt gacagtaaac attccttgtg ttccaactgc
     3721 tccccgcaca tcgattttac cttcactatt tagaggaaga ctgagatgcg gatttttgat
     3781 gtaacctttc acatctccct ttccatttgc atctactaca attcctgttg ctggaccatc
     3841 gccttggatt cgaacagtta gtttgtcttc tcctttgagc gtcgcaccaa gtagcagact
     3901 accgaccaat gtccgtccca aagcagctga ggaggtgtgc caagtatcat gacgtctttg
     3961 tgcctccgct acggtatttg ttgcttgaac ggcgtatgct cggacgaatc cgtcgtgagc
     4021 aagtgcctta accaaataat ctttcatttg ttatgtactc ctttttccgt ttttaaactc
     4081 atagtgaatc atactagcaa ctactgcttt tttcaacttt ggaatgcttc ttttaaaaat
     4141 aattcagaat catctagaaa acaggacatc accctattta caacttaaat aaagaaatct
     4201 cccgaaaacc ggtttttgtt ccggttttcg ggagtcatat cagatcaatt gacctatatt
     4261 tttttatttg tattgatcgt ctgaatgatt atcttctgat tgttgagaat ctgaatcatg
     4321 ttctgcttta ttttcctcat tcagttcatg ttttgcttct tcaaaatcac gtttttcttc
     4381 cgcttgtttt tcagcgtctt tttcttccaa agcacgtttt gcttcttcaa aagttttcgc
     4441 ttctttttcg cttggataat catttgactc agtaccttca ggcatcacac cgtgttcaaa
     4501 caatgacttg attgctttgg catccaatgt ttcgaattcc aaaagttttt cagcaatcag
     4561 tttatgctgt gcacgatgag cttcgatgat ttctcgagct ttatcatgag cttccatcaa
     4621 gatttttcgt acttcttgat caatctcgaa agcgacttgt tccgagtaag cttttgtttg
     4681 accatagtcg cggccaacaa acacttgatg gtttccttca tattgaacag gacctaaacg
     4741 atcactcatt ccatattctg ttaccatgct tcgtgctaga gctgtcgctt gctcaaagtc
     4801 attggaagca ccagttgatt ggacattgaa gatgatttct tcagctgtac gtccaccaag
     4861 taagcctacg atttgttcaa acatatcttc tttcgtcatc aagaactgat cttcttttgg
     4921 taaagcgatc atatatccgc cggcgcgtcc gcgagggatg atcgttactt tgtggacgac
     4981 ccgagcgcga ctcaatacta atccgacaat cgtatgacca gcttcgtgat aagcaaccat
     5041 ttcgcgttct tttttgctga ttacgcgatc ttttttagct ggacctgcaa tcacgcgatc
     5101 ttccgcttca tcgatatcag aagcatcgat tttcttttta tttcttctag cagctactaa
     5161 cgctgcttca ttcaagacat tttctaaatc tgctccggca aaacctggtg tctgttgagc
     5221 cacaactttc aagtcaacgt catctgccat cggtttattc cgtgcatgga ctcgcaagat
     5281 tgcttcacga ccttttacat ctggacgtcc aactaaaatc tggcggtcaa aacggcctgg
     5341 acgtagtaac gctgggtcta gtacatcaga acggttggtt gcagcaatga cgattactcc
     5401 ttcatttccg tcgaagccat ccatttctac aagcaattgg ttcaatgttt gttcgcgttc
     5461 atcatgtccg ccgcccatac cggcaccacg ttgacgacct accgcatcaa tttcatcaat
     5521 gaaaatgata gctggtgcgt ttttcttcgc tgtttcaaac aaatcacgaa cacgacttgc
     5581 tccaacaccg acaaacattt ctacgaaatc agaacccgag atcgagtaaa acggtactcc
     5641 agcttctccg gcaacagctt ttgctaataa ggttttacct gttcctggag gtccttctaa
     5701 aagtacacct gccgggatac gggcacctaa ttcaacaaat cggcgaggat ctttcagaaa
     5761 ttcaacaact tcaactagtt cttgtttttc ttcttccgca cccgctacat cagaaaaacg
     5821 tacgcgatta gcttttttgt ctgcttcttt ggcttttgat ttaccaaagt tcatgacacg
     5881 gccgccaccg ccaccgccgc cgccttgttg tcccatcatc atgtaaaaga aaaagatgat
     5941 gattacgatt ggcaagaagc taagcaatag ggaaacccat ataccgctgc ttgattcttc
     6001 ttttactgtt gtagcaactt tgttttcttt tgccagttct gttacttggc tcaatgttgt
     6061 atcatttggc aaaatgattg ttgtgaaatg ggtagtagtc gtatctgttg aacctaagat
     6121 agctaaccca ccgctgttag cgacggtctg ctgttctttg tattcaccag tgatcttata
     6181 tacaccattt gcaggctgca cttcaaattc tttgatcttg cctgcttgta gttgttctgt
     6241 gaattttgag tactcgatat ctgacgatcg ctggccgtta ttaccaaaaa tgaaataaac
     6301 gaccattacc atcgctaaga ttacgaggac ataatacaag gcatttttca tcatgccgtt
     6361 gttctttttg ttcatagttg ccctccctaa gcgtcaattt aatttaacag ttttttatca
     6421 atcctgacca ttctattgta ccataattta attgagatac agcattaatt tgattgataa
     6481 acactaggtt tcaatacacc gatgtacgga aggttacgat atgcttctgc ataatccaag
     6541 ccataaccga ccacaaattc atttggtacg ttgaagccaa cataatctgc ttcgatattt
     6601 actactcttc cttcaggttt gtcaagtagt gtaacaattt tgactgattt tgctttgcgg
     6661 tatttgaaaa gatccactaa ataagcaagt gttcttccgc tgtcaatgat atcttcaacg
     6721 atcaacagat cgcgaccttc tacattcgta tctaagtctt tgacgatttt tacttcccct
     6781 gacgagactg ttgcattccc gtagcttgaa acatccatga aatccaattc caaatacgta
     6841 tcgatagaac gaacgatatc tgccatgaat ggtaccgctc cttttaagac acctactact
     6901 aggggattgg tgttttggta aatttctgtt aactctttgc ctagttcttt gcaacgaact
     6961 tgtatctctt cttgtgagat caaaattctc tcaatatcct tttccaacat caggttctcc
     7021 ttccaattgc ttctttttga tatttgtaaa gaagtatgta gtgtatttta tcagtttctt
     7081 cacgaatact caaatatgaa aaggcaaaag gtatcaagct atatatattt cttttggcat
     7141 ctgtaatgat ccaagcatgc tgcctttgtg aattaggtac tttctgatcg ataaaatatc
     7201 tagatacttt ttttgtgaga ttctctgtta atttaatgcg gtctcctgct tttctttttt
     7261 cgacatacag ttgcacatca gcaggaatcc aaatgtcatg agaaaataga gaccatgaat
     7321 ctgttgcttt gatgtcccct gcattttcag gtttgaccag accaatccat tctgtttcat
     7381 tcaaatatat tccttgttct ggaatcagca acaagcgctg gtcttctgtc tggctagttg
     7441 cttgtttttc ttttttagag agtacaacaa aatgatactc tcttttcagt atccaattat
     7501 tgccaatact gagttgccat tggctttttt tctgttgcca ctggtctaaa atcgactcca
     7561 tttgttgttc tttcaaaata attcctgttt ttgagaatgc tttgtcgaaa aaggagtaaa
     7621 aaaaatagta tcgttcagca tctgtcattt cttcaatcat ttctgcagaa aatcgaaatt
     7681 gttcctccca ctgttcgact tccttttcga tcaactgtcc catatatttt tgaatgactt
     7741 ggtctgccca ttggatctgt tgcgaaaatt gctggaaatg tctcaacgcc tgtgtatttt
     7801 cttgtttcaa ttgaggaact accaaatgac gaagccgatt tctctggata tctaacaatt
     7861 ggtttgtctg atcctcaaag taaacaagct gcgattccgc tgcataagta tataggtctt
     7921 ctttgctaaa actcagcaac ggacgaatca gtctgcccgt agcaaatggt tgtacttctt
     7981 tgattccaga aaaagtattt aactgtcccc cacgaacgat tttcattaac accgtctcga
     8041 tttgatcatc tccgtgatga gcagtcatca acgtgtcata ttgtccttct tccatcacgc
     8101 gagtgaattc ttgataacga aagttacgag cttctgtttc tatggcggat ttggctggat
     8161 cttcccaaat acttacatat aaagggagat tcttctcttc acaatatgag cgaagatagg
     8221 cagcttcttc tgctgattct tctcttaatt ggtgattgat gtggacgata cctaaagaaa
     8281 aaccgatttg gcgttgcact tgtaaaagta aatggagcaa aaccatcgaa tcaacaccac
     8341 ctgataccgc tgctaaaatt ctcgatcctt gttgccagaa tccttgttca ttcccgtgcc
     8401 gaataaatga ctgctgtaat agcggtgctt ttttcccgcc tttttcaacg ttcatccttt
     8461 ccacaccctt tcttcctaaa aaaaagagag cgggaaaacg tctaaaacga caattaacct
     8521 gccctctgtt gatactatga cccaaatagt cgcactatca ttatttgcgg ctttttagct
     8581 gcgacgtccg ccacggccac cgcgctttcc ttctgtgttt cgcttcaatg aagataagcg
     8641 gtcatcgcta tctttcaaaa atgaactcat caatgaatcg aagtcttctt tttgagctga
     8701 aggttgactg cgagaaaaag attttttctg tgctggacga tttcggttgt cacggggatc
     8761 tttttgttga tattttttag gacggctttc ttctccagat tcagcaggtt gttcctgcgc
     8821 tttccggata gatagtccga tttttccatc atcatttact gaaataactt taactgttac
     8881 ttcatctcct acacttaaaa catcatggat gtcttttaca tagccatttg acacttcgct
     8941 aatatgaact agacccgttt ttccagcacc tagatcaata aacgcaccaa aatttgtgat
     9001 tcctgatact tttccttgca gttttgctcc tacctcgatt gacataaaaa aaatgttcct
     9061 cctaattatt tccctttttt atattcctgc ttattttgca gttgtagaac tagttgattg
     9121 atctgtttca tttgtcgagc tgtctgtatt tgattctaat ataggataaa ctttttcacc
     9181 gtcttttgag taaaagaatt tgcttctggc aagtttagct acatactcat catctttcag
     9241 caatgaaaca tcttggtgca gctgatcaac gtctgcgttg acttctttca tttcagtatt
     9301 tgcttcgact ctcaattcgt tcaattgatg catcctttgc atgtcatgga aaatctgcac
     9361 acctattatc gcgaaaatga ccaaagcacc aacaaaaatt gcagccagtc tgcgtcttcg
     9421 aaaaatcagt tgacgttgtt gttgctgaaa ttcagcatac tttttctttg catattccgt
     9481 atctagcgca gcgattttat tgcttttttt cgtcacttgg attcgctcca atctttttga
     9541 ttgtcttttt cattatacca atatcgtgaa agcttgtcta atctatttct ctagtgtttc
     9601 tgtgcgattt tcagaaataa tttcgtacat tttttgtgca tcttcttttt tggttgattc
     9661 atgtaattcc gtaattttca cttccaacgt tttgttgcca aactggactt tcaaaatatc
     9721 gcctactttg acatcactgg aagatttcgc caatttaccg ttgatttgaa ttcttccttt
     9781 atcagctact tcttttgcta ctgatcgtct tttgatgatt cgggaaattt ttaaaaattt
     9841 atctaatcgc atatcttatt cctcactttt taattttttt atttaatcgc aggattttct
     9901 taccaaaagg caacattagc cattctcgta ctgtaaacag acgaagacga atcactgcat
     9961 aaatgaacac agaagccccg atcccaactc ctatcacgct gacaaaaaaa gcagtggaac
    10021 gatgagttac aggaccaaat actatattca atacaccata atacagcagc agtactactg
    10081 tcatacaaag aaggctgaac atcaacttcc gtccaaaatg ccgttctttc caaaaagcat
    10141 ttagctcttt tgtttcaatt cgaacaaaat accaaagcgt cacacctaat cctaaaaggg
    10201 tagacaggct tgcacctgct gttcctaata aaccggtcag aaaagaagta gtcaatcctt
    10261 ttaccaatag tccccatcct gcgcctttta aagaagggcg aaacgagttc ttgctttgag
    10321 caatactttg ataagcttgg atgactgcag tgaaagcaat ggaaaaaaca aataatacaa
    10381 gcgctgcatt tcccgcataa tctttaaaca acgcataatt gatataaggg agcagcaagg
    10441 ctaatcccaa agacgcagct aatgctaaag cggtcgtcaa tctaagatag attttggctg
    10501 tctgtaaaaa ctgacgatag atccggttgg tcaaatgacg agtcaacgca ggcaggaatg
    10561 tcgcacttaa tgcagtggca atcactaaac ctaattgtac taaaggctga cctcgatcat
    10621 agaccccttt tgctatcttc gcaccgtatt ctgacagtcc gtaaacttcc aaagcatttt
    10681 ttacaaagaa agagtcgatc agctggaaaa agataagcaa tccactataa atcgaaacca
    10741 gtccgccttc taccagaaaa cgccggatca agggctttga cggctgtttg aacaatggaa
    10801 accgtcggaa gcttaacgct cctccatgaa tcttttgttc atagtagtga aggatcccat
    10861 atgcgcataa accgccaaaa acagctccac tcattgctgc cgtccctgtt tggtacaccg
    10921 tccatccaag ctttctaaaa gcaaacgcac tacagacaat cacaaaaaca cgaacgaact
    10981 gctcaactac ttgagaaaca gcagttggtt ccatcaagaa tcttccttgg aaattccctc
    11041 gataaaaaga tagaccaggc atcagcaaaa aggtaaagga aactacttgg atcagcggtt
    11101 tcaattgtat gtctcccatc atccaagcaa tccagctgct aaatgcaaag acaaacaacc
    11161 acaaaaagac acctgtccaa aatactaagg gataaagatt ttgcaaagct tgctttcgct
    11221 cgaaaggatt ctttctttct gcaacgtact ttgatatgaa ctgcggaaga ccggataaag
    11281 ctaaagtcat tgctatccca taaattggat aaacttgctg ataaacataa aaaccttcgt
    11341 cgccgactaa gttttggagg ggaattcggt aaagggcact taatactttt gctatgaaag
    11401 aagcaatcgt caatacaaat gctccttgca tgacgcgccg catttcttta tgatccaaat
    11461 cattgctcct tcctacgctg acagatattt ttgctcacgc aacgctttga caaacttttg
    11521 gatttcctga agccatactg cttcttccat ttggttgggg atcgtcaagc gtatcaccat
    11581 ctgttctttt tcaacgccta gactggcttt tagttttgtc gctgtcagtg cttcgaataa
    11641 ctgttccaca ctatagcgtt ttgttccaac tttacttaga gtaaagacta tatgcgcctc
    11701 ctgtttcctt atcgtttcta ggagcgctcg atcgccatcc attttgatct gcccaattgt
    11761 caagagatgt gctacttctt ctggatattc tccaaaacgg tcgagcagat catcttctag
    11821 ttcatctagc atttcttgcg aatcaagttc acgaattcgt ttatagatct cgattttctg
    11881 ccgttgatct gccacgtatg tttcaggtag atacgcatcg acaccgaggt cgatctcaac
    11941 agtggtcttc tccacttgac tattctttcc ttgtttacga gaaacagctt cgcttagcat
    12001 ttgagaatac atatcgaaac cgactgcatc aataaatcca tgctgctgag cacctaagag
    12061 attacctgca ccgcgaatgg aaagatcccg catcgcaatc ttgaatccag atcccagttc
    12121 tgtaaaatct ttgattgcct gaagacgttt ttcactgact tcgttaagga tcttctgctg
    12181 ctcatacata aagtaagcat atgcgacacg gctgcttctt cctacccgcc ctcgcaattg
    12241 gtataacgtg gataatccca tataatcagc gttctcaacg aacaacgtat ttgcgttcgg
    12301 gatatctact cctgtttcaa tgatggttgt agtgacaaga acatcatatt gaccttcaat
    12361 aaattcaaaa agcgtatttt ctaattgcgc ctctgtcatt tgcccatgag cataaccgat
    12421 ccgtgcttcc ggaacgagtt cttggatttc ttctactttt tgttcgatcg tttctacccg
    12481 attatataaa tagaatactt gtccacctcg tcccatttct cgatggattg cttcgcgaat
    12541 agccccagga tttttttcca taacatacgt ttggatcgga taacgattcg caggcggtgt
    12601 ttcgattact gaaagatccc gcacgcctaa catcgacata tgcaaagttc tcggaatcgg
    12661 tgttgcagtc agagtcaaaa catccacttg tgcacgtaac tgtttcaatc gttccttatg
    12721 ttttacacca aaacgttgtt cttcgtcaat gatcagtaga cccaaatcgc taaattcaat
    12781 atcttttgac aaaattcggt gggttcctac aacaatatca acttgacctg tacgcagttg
    12841 ttcgatggtt tctttttgtt gctttcttgt tctgaaacga cttaatagcc cgatatttac
    12901 ggggaaacct tcaaatcttt ccagcatcgt ttcatagtgc tgctgtgcca agatcgttgt
    12961 tggaactaaa aaggctactt gtttgctttc ctttatcgct ttaaaggccg ctcgaagcgc
    13021 tacttctgtt ttcccataac caacatcacc tacgagtagt cgatccattg gtttttcttt
    13081 ttccatatcc cgtttgatct ccgctgcact tctcaactgg tcatcagttt cagagtaggg
    13141 gaaggcattt tcgaattctt tttggtaggc gtcatcaggt ccaaaagcat atcctttttc
    13201 tgactctctt gcggcatata atttgattag atcgtcagcg atatcctcga tttttgaaga
    13261 gacttttcgc ttggttttcg tccattcact tccgcctagc ttattgatgc gaggcgcttt
    13321 ggcttcagag gcgacatatt tttggatcag attcagttga gtaacaggaa taaacagttt
    13381 gtcgtcgttt tgatacaaaa tagtcatata gtcttggtga acgccatcta cttctagcgt
    13441 ttccatccca atgtatttcc cgataccatg attggcatgg acaacgtaat ctcctgcttt
    13501 taactcatta tagcttttca atcgctctgc gtttgttact gtttgacgtc tcgtttgttt
    13561 tttcgtcgtt ttttggaaaa tttctttttc tgtgatagct actatttttt cttgaggaag
    13621 ttcaaaacca gcctgcaaag ctccttcaac taattgtacc tgtcctctga tcaatgcatt
    13681 tttttctgtt tctactacta atatgtcttc atctcgaagc atttgttctg ctttgcgtat
    13741 tctgtccttt gtcggaataa agatcacaac agtctgtttt tgcttgcgcc aacggtccat
    13801 ttctgttttc agcaatggca tttgtccgaa gaactgttgc atcgtccgat attgaaaatt
    13861 atggattgct tggaaacgaa gatttcccat ccctttttgg aagagtgaga aaaaggttgt
    13921 ggtgaacttt tccttctgca cgattttttg tatatctgct gaaaatgtct gctcagaaaa
    13981 cacccgcaat tcgctgattt tttgagtctg ccattctgcc gcttcccttt cgatttcccg
    14041 atttgtttcc atgattcgct gataatcatc aacgactagt aaactatttg aagggaaata
    14101 atccaagatc gtcacagatt cttggtaaag aagatctgag tagaattttg cttcttctgt
    14161 cgggacacct gcttgccagc ttgaagtcag ctgaccgaag taatcttgca aaaactcgcg
    14221 gtctgcttct tctgtggtcg cagcaagccg tttttctagc attttttgca tttgctctat
    14281 tccatgttct agatcctctg gtccgtagac ttgttcactc gttggggaaa cccagatttc
    14341 ttccgttttt tcgattgagc gttgtgtttc tgcttcaaaa taccgcatag aatctatctc
    14401 tacatcaaat agttctacac gaacaggata ggttgtcgtt aacgggtaaa tatcgataat
    14461 gcttcctcga atactgaatt ctcccggttt tgcgaccatc tcttctcgta catagcccat
    14521 caaagtcaat tggcgaggaa gattttccac gtctatctca tcaccaatat gccaattcaa
    14581 ttgcgcctgt agccaagttt tcttattcgg cagtcgtttg tgcaaagatg cgactggcaa
    14641 caaatagata cctgcttttt tttcttgaag tgcgttaagc gttgcaactc tctcgcctcg
    14701 cgcttctgga gaagaaaagg ccatttcagc agaaagcact tcatccactg gaaataaatg
    14761 gatctgatct tgcggaatca catgttgcag atcttctgct aactgattcg cataatacaa
    14821 atttggcatc agaataatca tgtttttatt tgttttctta aacgctcgag caaatagaag
    14881 tgttttcgct gagcccgcta atcctgtgat tagttgtcta ggcatttttt cttctaaatt
    14941 gctgacccat tcttttactt gcggatcttt tccgactaat tgaataatat ccattccttt
    15001 tcctccttcc tcaattgaat cgattcatcg catccacgaa cgaatgccct tctatgagat
    15061 attcgactgc tttaacagat tgacacatgc tttcttctat caaaggctga ttttctttgc
    15121 tgaaaggact aagcacatgc tggactactg tttttttccc ttctggcctg ccgattccca
    15181 ccttgattcg atcaaagaca ttcgtattca aatgggaaat aatacttttg ataccgttat
    15241 gcccgccggc acttcccttt tgacggagcc ggatttttcc cactgccaga tccaaatcat
    15301 cgtagattac gactaattcc tctggataaa tcccaaaata agtcattagt gggccgactg
    15361 cacgaccaga ttcattcatg aatgtttgtg gtttgactaa aaggatcttt tctccattat
    15421 gaaaaaattc ggcaacttct gcttcgaacg gatttttttt gaaagtagct tgatgctcgc
    15481 gtgcaatttt atccacgacc ataaagccga tattgtgttt tgtatattgg tatttggtac
    15541 ctgggtttcc taacccgacg atcattttca tttttgtttt ccacctaatt ctaaaaagca
    15601 gcacaaaagg ctggactgct aattagcggt ccagtccatc gagctttttt cttttttaca
    15661 cttgtcaatg catagtataa cacaaaacaa ggcgtttcgg ctaaaagaga atcgttaata
    15721 aatgataaag aaattaaaaa tttcttcaaa gtttaatgaa ataattgata tataaaaata
    15781 gtgaaataac gcacatgtaa gcttttacat ataatacaac cttgttatct gttatatttt
    15841 atagccaaat agcgtgacat ggggcttgag aaatgttagc ataagcatgg gaaaaaacaa
    15901 atcaatggaa ggattgatac taaatgactg cagctgcaga aaaaaaagat catcaaaaag
    15961 ttattcttgt tggagacggc gccgtaggtt ctagctatgc ctttgctcta gttactcaaa
    16021 atatcgctca agaagtaggt attgtggata tcaatactgc taaaactgaa ggagacgcaa
    16081 ttgacttgtc tcacgctttg gcatttactt cacctaaaaa aatctattct gctacttacg
    16141 cagacgcaca tgatgctgac ttagttgtta ttacagctgg cgcaccacaa aaaccaggtg
    16201 aaactcgttt agacttagta aacaaaaacc taaaaatcaa ccgcgatgtt gtcacacaaa
    16261 tcgttgaatc aggatttaac ggtatctttt tagttgccgc taacccagta gatattttga
    16321 cttactcaac ttggaaattc tcaggtttcc ctaaagaacg cgtaatcggt tcaggtacct
    16381 ctcttgactc agctcgtttc cgtcaagcac ttgcggaatt agttgacgtt gatgcacgta
    16441 acgtccatgc ttacatctta ggtgaacacg gagattcaga attcccagtt tggtcacatg
    16501 caaatgttgc cggcttgcaa atctatgaat gggtaaaaaa caacccagaa atcgatgaag
    16561 aagctatggt taacctattc tttggcgtac gtgacgctgc ttacacaatt atcgagaaaa
    16621 aaggcgctac tttctatggt atcgcagtag cattagcacg tatcactcgt gccatcttag
    16681 atgatgaaaa tgctgttctt cctttatcag tattcatgaa tggcgaatat ggattgaacg
    16741 acatctacat tggtgcacct gctgttatca accgtcaagg tatccaaaaa gtaatcgaaa
    16801 ttcctttaag tgacagcgag caagaccgta tggctgcttc tgctaagcaa ttaaaagaaa
    16861 ttctagatga agcttttgct aaactagacg ctgaataatc tttgaacatg acggaaaaca
    16921 tctattcggt gttttccgtt tttttatttt atcaccctta tggtttctaa attttttcat
    16981 aaagtagaga taaccttcgt cttttcctct aaattatgct atcatgagac cgttccaccc
    17041 taaactttta cttaaggagt tgtcgatatg acaagatctt cgcatcgaaa aaaatcgaaa
    17101 catgttgctt tgctttcagt tcttggtgtt ctcttagtgc tgatcagctt ttatacctat
    17161 cgcagtacat actacacaaa gcattttttg ccgaatacag aaataaatgg aataaatgtc
    17221 agcaatctaa cagtcgaaaa agcaaatgaa aaattaaaag aagcttattc tgataaaaaa
    17281 ctggctatta aagaagatgg aaaagtctgg caggaagtac caaaatccga gctcggctac
    17341 aaagatgatt tcacttcgga gctttctccg atcctaaatg aacaaaacgg atggacttgg
    17401 ggaatgactt atgtctcagc ggcagaaaaa caagaaatag atcctgtcag tcaaaaccac
    17461 caaaaactag acactaccgt tcagacattg acaactaagc tgactgattt gaacaaagac
    17521 cggacaccta ctactgatgc aacgatcgaa aaagcaggtg actctttcgt tatcaagcct
    17581 gaagtcaatg gaaatacgat cgacgtcgat gctgcggtca aacaattaaa aagtgcggta
    17641 aactctggta aagatacaat tgaattgact gaattcaaag aaaaaccaaa agtaacatct
    17701 gaagacagct cactgaaaga acaactcgca tccatgaatg ctatcgcaaa tgtaaaagct
    17761 acttacagta taaacggtga gactttccag atcccatcaa gtgatattat gagttggctg
    17821 acctacaatg atggaaaagt cgatttagat acagaacaag tacgtcaata tgtaacagat
    17881 ttaggcacaa agtataacac aagtacgaat gacacaaaat ttaaaagtac aaaaagagga
    17941 gaagttacgg tcccagttgg cacttacagt tggacgatcc aaacagacag tgagacagaa
    18001 gcattgaaaa aagcgattct ggcaggccaa gattttactc gttcacccat tgtgcaaggt
    18061 ggcacaaccg ctgatcatcc attgatcgaa gatacgtata tcgaagttga tctggagaac
    18121 cagcatatgt ggtattacaa agacggaaaa gtggcccttg aaacagatat cgtttccgga
    18181 aagccaacta cacctacacc tgctggagtc ttctatgttt ggaataaaga agaagatgca
    18241 actttaaaag gaacaaatga cgatggaaca ccttacgaga gtccggtaaa ctactggatg
    18301 ccgattgact ggacaggtgt tggtatccac gattcagact ggcaacctga atacggtgga
    18361 gacttatgga aaacccgcgg ttcacacggc tgcatcaaca ccccaccaag tgtaatgaag
    18421 gaattattcg ggatggtcga aaaaggaacg cctgtattgg tcttctaaca aaaataatat
    18481 ataaaaagaa agcacccgta cgattggtac aggtgctttc tttttattat ctaacaatgt
    18541 gctacttaca tttcagcagc agtaaagact tctgaaacat catcatcgtc ttctagctta
    18601 tctactagac gttccaattg ctctttttgt tcctcattta aatcaatcgt cgtttgtgga
    18661 atcatcgtca attctgcttg cgccaaagtg aactcttttt ccaactcatc tcgtacagca
    18721 gcaaaatctt ctggtgcagt ataaatctcg aatacttcag gagaagtttg cagatcttct
    18781 gcccccgcct ctaatacatc ctcaaacatc gtctcttcat caacagtcaa cccatcgcgt
    18841 tcaatagcga tatatccttt gcggtcaaac aagtaactga ctgaacccgt ttctcccagt
    18901 gatccaccgt ttcgagtaaa agctactcgg acatttgtcg ctgtccgatt acggttatct
    18961 gtcaatgcat ggactaagat tcctactcca cccggaccat atccttcata cgtgatttca
    19021 tcgtaattcg tttcatctac ggcactcgta gcctttttaa tcgcccgttc cacattgtcg
    19081 ttaggcatat ttgcagcttt tgctttgtcc acaacaagcc gcaaagctgg attcatagat
    19141 ggatcaggac cgcctgcttt agctgccata taaatttcac gagaaagttt ctgaaagact
    19201 ttccctcgtt ttgcatcttg ggcatttttt cttccttgga tattatgcca tttactatgt
    19261 cctgacatga tttttcctcc cttgttcttt tgagaataca cttctattct agtactattt
    19321 cacaaaaaaa caaagccaca gccaatctat aaacgcttct tgaatgccgc aaaaagcaca
    19381 cataatatca cagcagtcag agcaccgatc gaatccaaca tgacatcttg gaacataggt
    19441 gttcgtccac cggtcatcat ctgatggaat tcgtccaaac ctgcgtaccc tgttgctgaa
    19501 agccaagcaa ggatcgctgt cagccaccaa atttttagct tggggaaaac acctaaaaac
    19561 aagctgcctc ccaaaataaa ataggtaccg aaatgtgcgc ccttccttaa aaagaactct
    19621 aaaaatttgg cgtagcccag atggttgata ctcaccatac ttccaccata attaaaacgt
    19681 atacccgcta atgtgtcttt aaatggctga ttaggcagcc agctttctaa tcgcgaaact
    19741 tgtgattgct gtccgtaagt ttgagaagaa ctgtaaaata agattataac gactatcagt
    19801 gcgattacta aataaatatt tccgtttttc atttgttttt ttgaagaatt cataagtcac
    19861 ctctgtattt tatcttagac tttttttgaa gaaacaacaa tcattttcat agagacttct
    19921 tccataaaaa agtgctatac ttaattttgt attacaatat gaagggagtt ttttcatgag
    19981 ttattcatta aattggaatc tagattctat tttttccgga ggcagtcatt ctgatgcttt
    20041 gaaccaacgg atgaagcaat tggaagacca aatgaacgag tattatcacc gcgtaacaaa
    20101 atggtcacct agttctgaca atgcagagca gttaaacgct attcttcaat tgcaagaaac
    20161 gattacgaat ggttttaccc aatgcagcag ctatattact gcattgcttt ctgccaatgt
    20221 caatgattca gatgcaaagg tcttatcagg caagctttac gcaatgctgc ctcgtctgca
    20281 atcagccgaa actgtattgt caaagaaatt tgcagaaata tctgacaatg attggaatca
    20341 gatgctttct caatcttcgt ttgaaacaat tgcttttcgt ttaaacgaga ttcgtcgtga
    20401 tggctcacaa ttattatcag aagctgaaga aaacattata aatacgcttt ctttagatgg
    20461 tctaaatgct tggagcagtc attatgatac gattgtagcc agcatttcga ttcccttcga
    20521 acaagatgga caagtagtcg aattatctgc tggacaagcc ttcaataaga tgatgggcga
    20581 tccagatcca aaagtcagag aaacactttt cgcagcatgg gaagaagcct ggaaagaaaa
    20641 ggcacccctc ttcacggata ctttgaatca tttagatggt tttagacttt ctgactatga
    20701 acttcatggt gtcacagatt ttctacaaaa accattagag tacaatcggt tgaagaaaga
    20761 aacattgtct gtcatgtggg atacaatcca gaaaaacaaa caaccccttg ttaattactt
    20821 gactagaaaa gctaatcttt tcggtaaaga aaaaatggaa tggcaagatc aagatgcacc
    20881 tattatttta ggcgatttga aagaaaaaac ctttagtttt gacgaagcag cagcatttat
    20941 tatcgaaaac tttaacaaat tcagtccaaa aatggctaaa tttgcccaat ctgcttttga
    21001 aaagagctgg atcgaagcag aagatcgtcc tgggaaacgt cctggtggtt attgtactga
    21061 gttaccagaa acaaaagaat caaggatctt tatgacctac agtaattctg ttaatgaggt
    21121 tgcgacactt gctcatgaac taggacatgc tttccatagc agcacgatgt gggatttacc
    21181 ttctttaaat cgcgaatatg ccatgaatgt cgcagaaaca gcaagtacat tcgcagaatt
    21241 gatcgtagcc gatgctacat taaaagtcgc aaaaacaaaa gaagaaaaaa tcaatttgct
    21301 agatacaaaa ctgcaaaatg ctttagctat gttcatgaat atccattccc gattcatttt
    21361 tgaaaatcga ttctatgaag cacggcaaga aggattagtc tcagaagata agatcacaga
    21421 gatgatggtt gaggcgcaaa aagaaggcta tcaaggagct ttagctactt accatccata
    21481 tttctgggct gcaaaattgc atttctttat tgatgacgta ccgttttata acttcccata
    21541 cacatttggc tacctcttta gtttgggcat ctatgcttac gccaaccaaa agggagcaaa
    21601 ttttgaagaa gaatatatcc atttactacg cgatactgcc tcaatgacga cggaagatct
    21661 tgcgaagaaa catttgaatg tcgatcttac ccaaccagat ttttggcaag caggtatcga
    21721 catggtgact gaagatatcc aaacgttcat ggaattgaca gaagaattta tctgatttct
    21781 attttatcac aataaaaaaa gagttagtcc agaatgggct aactcttttt g
//
LOCUS       DACCZY010000044        21043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCZY010000044 DACCZY010000000
VERSION     DACCZY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.044"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            20..1186
                     /locus_tag="IVZ67_001342"
     CDS             20..1186
                     /locus_tag="IVZ67_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8165425.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1416..2063
                     /locus_tag="IVZ67_001343"
     CDS             1416..2063
                     /locus_tag="IVZ67_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8165426.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2147..3091
                     /locus_tag="IVZ67_001344"
     CDS             2147..3091
                     /locus_tag="IVZ67_001344"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8165427.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3226..4677
                     /locus_tag="IVZ67_001345"
     CDS             3226..4677
                     /locus_tag="IVZ67_001345"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8165428.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4698..5534
                     /locus_tag="IVZ67_001346"
     CDS             4698..5534
                     /locus_tag="IVZ67_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8165429.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5534..6193
                     /locus_tag="IVZ67_001347"
     CDS             5534..6193
                     /locus_tag="IVZ67_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8165430.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6319..7008
                     /locus_tag="IVZ67_001348"
     CDS             6319..7008
                     /locus_tag="IVZ67_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165431.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7014..7970
                     /locus_tag="IVZ67_001349"
     CDS             7014..7970
                     /locus_tag="IVZ67_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8165432.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8014..8526
                     /locus_tag="IVZ67_001350"
     CDS             8014..8526
                     /locus_tag="IVZ67_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165433.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8782..9330)
                     /locus_tag="IVZ67_001351"
     CDS             complement(8782..9330)
                     /locus_tag="IVZ67_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8165434.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9419..10600)
                     /locus_tag="IVZ67_001352"
     CDS             complement(9419..10600)
                     /locus_tag="IVZ67_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8165435.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(10836..12344)
                     /locus_tag="IVZ67_001353"
     CDS             complement(10836..12344)
                     /locus_tag="IVZ67_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8165436.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            12503..12694
                     /locus_tag="IVZ67_001354"
     CDS             12503..12694
                     /locus_tag="IVZ67_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165437.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            12897..13814
                     /locus_tag="IVZ67_001355"
     CDS             12897..13814
                     /locus_tag="IVZ67_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8165438.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            13807..14880
                     /locus_tag="IVZ67_001356"
     CDS             13807..14880
                     /locus_tag="IVZ67_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165439.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15028..15546
                     /locus_tag="IVZ67_001357"
     CDS             15028..15546
                     /locus_tag="IVZ67_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165440.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            15527..15919
                     /locus_tag="IVZ67_001358"
     CDS             15527..15919
                     /locus_tag="IVZ67_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8165441.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            15995..16930
                     /locus_tag="IVZ67_001359"
     CDS             15995..16930
                     /locus_tag="IVZ67_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8165442.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17063..17842
                     /locus_tag="IVZ67_001360"
     CDS             17063..17842
                     /locus_tag="IVZ67_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8165443.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            17835..18152
                     /locus_tag="IVZ67_001361"
     CDS             17835..18152
                     /locus_tag="IVZ67_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8165444.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            18396..20006
                     /locus_tag="IVZ67_001362"
     CDS             18396..20006
                     /locus_tag="IVZ67_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8165445.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            20163..21011
                     /locus_tag="IVZ67_001363"
     CDS             20163..21011
                     /locus_tag="IVZ67_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8165446.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6843 a         3743 c         4210 g         6247 t
ORIGIN      
        1 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
       61 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
      121 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
      181 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
      241 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
      301 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
      361 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
      421 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
      481 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
      541 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
      601 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
      661 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
      721 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
      781 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
      841 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
      901 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
      961 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
     1021 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
     1081 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
     1141 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
     1201 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
     1261 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
     1321 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
     1381 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
     1441 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
     1501 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
     1561 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
     1621 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
     1681 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
     1741 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
     1801 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
     1861 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
     1921 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
     1981 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
     2041 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
     2101 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
     2161 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
     2221 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
     2281 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
     2341 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
     2401 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
     2461 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
     2521 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
     2581 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
     2641 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
     2701 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
     2761 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
     2821 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
     2881 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
     2941 tggcgcaagc atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc
     3001 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
     3061 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
     3121 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
     3181 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
     3241 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
     3301 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
     3361 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
     3421 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
     3481 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
     3541 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
     3601 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
     3661 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
     3721 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
     3781 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
     3841 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
     3901 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
     3961 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
     4021 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
     4081 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
     4141 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
     4201 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
     4261 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
     4321 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
     4381 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
     4441 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
     4501 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
     4561 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
     4621 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
     4681 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
     4741 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
     4801 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
     4861 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
     4921 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
     4981 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
     5041 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
     5101 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
     5161 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
     5221 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
     5281 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
     5341 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
     5401 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
     5461 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
     5521 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
     5581 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
     5641 aaatttggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
     5701 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
     5761 gttatatttc gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
     5821 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
     5881 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
     5941 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
     6001 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
     6061 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
     6121 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca
     6181 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
     6241 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
     6301 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
     6361 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
     6421 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
     6481 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
     6541 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
     6601 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
     6661 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
     6721 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
     6781 cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa
     6841 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
     6901 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
     6961 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
     7021 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
     7081 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
     7141 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
     7201 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
     7261 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
     7321 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
     7381 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
     7441 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
     7501 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
     7561 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
     7621 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
     7681 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
     7741 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
     7801 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
     7861 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
     7921 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
     7981 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
     8041 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
     8101 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
     8161 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
     8221 tcaggcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
     8281 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
     8341 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
     8401 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
     8461 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
     8521 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
     8581 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
     8641 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
     8701 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct cggcttatcc
     8761 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
     8821 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
     8881 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
     8941 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
     9001 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
     9061 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
     9121 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
     9181 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
     9241 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
     9301 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
     9361 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatttt
     9421 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
     9481 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
     9541 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
     9601 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
     9661 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
     9721 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
     9781 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
     9841 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
     9901 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
     9961 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    10021 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    10081 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    10141 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    10201 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    10261 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    10321 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    10381 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    10441 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    10501 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    10561 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    10621 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    10681 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    10741 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    10801 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    10861 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    10921 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    10981 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    11041 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    11101 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    11161 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    11221 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    11281 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    11341 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    11401 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    11461 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    11521 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    11581 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    11641 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    11701 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    11761 tggctttcat gatacagttg gttcaagcaa gtagcgagct ttccacttcc tgggccagga
    11821 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    11881 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    11941 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    12001 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    12061 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    12121 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    12181 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc
    12241 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    12301 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    12361 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    12421 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    12481 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    12541 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    12601 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    12661 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    12721 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    12781 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    12841 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    12901 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aacgcggatc
    12961 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    13021 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    13081 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    13141 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    13201 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    13261 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    13321 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    13381 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    13441 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    13501 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    13561 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    13621 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    13681 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    13741 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    13801 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    13861 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    13921 gaaatcccta tgctagagga aacaaaaata catgaaaaag aaaaagtaac tccgacgaat
    13981 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    14041 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    14101 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    14161 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    14221 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    14281 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    14341 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    14401 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    14461 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    14521 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    14581 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    14641 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    14701 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    14761 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    14821 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    14881 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    14941 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    15001 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    15061 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    15121 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    15181 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    15241 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    15301 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    15361 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    15421 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    15481 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    15541 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    15601 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    15661 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga
    15721 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    15781 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    15841 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    15901 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    15961 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    16021 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    16081 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    16141 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    16201 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    16261 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    16321 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    16381 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    16441 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    16501 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    16561 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    16621 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    16681 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    16741 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    16801 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    16861 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    16921 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    16981 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    17041 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    17101 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    17161 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    17221 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    17281 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    17341 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    17401 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    17461 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    17521 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    17581 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    17641 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    17701 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    17761 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    17821 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    17881 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    17941 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    18001 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    18061 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    18121 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    18181 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    18241 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    18301 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    18361 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    18421 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    18481 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    18541 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    18601 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    18661 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    18721 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    18781 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    18841 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    18901 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    18961 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    19021 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    19081 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    19141 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    19201 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    19261 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    19321 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    19381 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    19441 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    19501 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    19561 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    19621 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    19681 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    19741 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    19801 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    19861 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    19921 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    19981 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    20041 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    20101 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    20161 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    20221 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    20281 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    20341 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    20401 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    20461 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    20521 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    20581 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga
    20641 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    20701 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    20761 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    20821 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    20881 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    20941 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    21001 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DACCZY010000045        20400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCZY010000045 DACCZY010000000
VERSION     DACCZY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.045"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(387..1853)
                     /locus_tag="IVZ67_001364"
     CDS             complement(387..1853)
                     /locus_tag="IVZ67_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165447.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVZ67_001365"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IVZ67_001365"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8165448.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4370..4549)
                     /locus_tag="IVZ67_001366"
                     /pseudo
     CDS             complement(4370..4549)
                     /locus_tag="IVZ67_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(4753..5112)
                     /locus_tag="IVZ67_001367"
     CDS             complement(4753..5112)
                     /locus_tag="IVZ67_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8165449.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="IVZ67_001368"
     CDS             complement(5142..5483)
                     /locus_tag="IVZ67_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165450.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="IVZ67_001369"
     CDS             complement(5480..6154)
                     /locus_tag="IVZ67_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8165451.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="IVZ67_001370"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="IVZ67_001370"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8165452.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="IVZ67_001371"
     CDS             complement(8668..9858)
                     /locus_tag="IVZ67_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8165453.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="IVZ67_001372"
     CDS             complement(9879..10406)
                     /locus_tag="IVZ67_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8165454.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="IVZ67_001373"
     CDS             complement(10453..13242)
                     /locus_tag="IVZ67_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8165455.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="IVZ67_001374"
     CDS             complement(13389..13589)
                     /locus_tag="IVZ67_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8165456.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="IVZ67_001375"
     CDS             complement(14042..14362)
                     /locus_tag="IVZ67_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165457.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="IVZ67_001376"
     CDS             complement(14865..15983)
                     /locus_tag="IVZ67_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165458.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16454..17761
                     /locus_tag="IVZ67_001377"
     CDS             16454..17761
                     /locus_tag="IVZ67_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8165459.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="IVZ67_001378"
     CDS             17882..19081
                     /locus_tag="IVZ67_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8165460.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="IVZ67_001379"
     CDS             complement(19108..20376)
                     /locus_tag="IVZ67_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8165461.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5906 a         4344 c         3559 g         6591 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    19141 tattggcaaa aagagtatcc cttttctgac cgctacttgc tgagcagaat aaataccgct
    19201 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    19261 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    19321 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    19441 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    19501 tccattaaat gattcctgca gtaatggaag actcaatccg agataacgtt gtgtttgcaa
    19561 aatcaatgct gcggctacaa ctacagcccc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca taaaatttgc
    19801 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc
    19981 tgtattatct gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc
    20041 tccaaataaa tgggtagtta tggttccaaa cagtgtaagt ggaataagtc gcaacgggat
    20101 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    20161 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    20221 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    20281 taagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    20341 taaaatgcgt actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
//
LOCUS       DACCZY010000046        20240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCZY010000046 DACCZY010000000
VERSION     DACCZY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.046"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            300..608
                     /gene="rpsJ"
                     /locus_tag="IVZ67_001380"
     CDS             300..608
                     /gene="rpsJ"
                     /locus_tag="IVZ67_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8165462.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            639..1268
                     /gene="rplC"
                     /locus_tag="IVZ67_001381"
     CDS             639..1268
                     /gene="rplC"
                     /locus_tag="IVZ67_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8165463.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1296..1919
                     /gene="rplD"
                     /locus_tag="IVZ67_001382"
     CDS             1296..1919
                     /gene="rplD"
                     /locus_tag="IVZ67_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8165464.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1919..2209
                     /gene="rplW"
                     /locus_tag="IVZ67_001383"
     CDS             1919..2209
                     /gene="rplW"
                     /locus_tag="IVZ67_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8165465.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2248..3081
                     /gene="rplB"
                     /locus_tag="IVZ67_001384"
     CDS             2248..3081
                     /gene="rplB"
                     /locus_tag="IVZ67_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8165466.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3124..3402
                     /gene="rpsS"
                     /locus_tag="IVZ67_001385"
     CDS             3124..3402
                     /gene="rpsS"
                     /locus_tag="IVZ67_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8165467.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3425..3772
                     /gene="rplV"
                     /locus_tag="IVZ67_001386"
     CDS             3425..3772
                     /gene="rplV"
                     /locus_tag="IVZ67_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8165468.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3786..4442
                     /gene="rpsC"
                     /locus_tag="IVZ67_001387"
     CDS             3786..4442
                     /gene="rpsC"
                     /locus_tag="IVZ67_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8165469.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4445..4879
                     /gene="rplP"
                     /locus_tag="IVZ67_001388"
     CDS             4445..4879
                     /gene="rplP"
                     /locus_tag="IVZ67_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8165470.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4869..5057
                     /gene="rpmC"
                     /locus_tag="IVZ67_001389"
     CDS             4869..5057
                     /gene="rpmC"
                     /locus_tag="IVZ67_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8165471.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVZ67_001390"
     CDS             5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVZ67_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8165472.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5406..5774
                     /gene="rplN"
                     /locus_tag="IVZ67_001391"
     CDS             5406..5774
                     /gene="rplN"
                     /locus_tag="IVZ67_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8165473.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5811..6119
                     /gene="rplX"
                     /locus_tag="IVZ67_001392"
     CDS             5811..6119
                     /gene="rplX"
                     /locus_tag="IVZ67_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8165474.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6147..6686
                     /gene="rplE"
                     /locus_tag="IVZ67_001393"
     CDS             6147..6686
                     /gene="rplE"
                     /locus_tag="IVZ67_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8165475.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6705..6890
                     /locus_tag="IVZ67_001394"
     CDS             6705..6890
                     /locus_tag="IVZ67_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8165476.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6926..7324
                     /gene="rpsH"
                     /locus_tag="IVZ67_001395"
     CDS             6926..7324
                     /gene="rpsH"
                     /locus_tag="IVZ67_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8165477.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7356..7892
                     /gene="rplF"
                     /locus_tag="IVZ67_001396"
     CDS             7356..7892
                     /gene="rplF"
                     /locus_tag="IVZ67_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8165478.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8053..8409
                     /gene="rplR"
                     /locus_tag="IVZ67_001397"
     CDS             8053..8409
                     /gene="rplR"
                     /locus_tag="IVZ67_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8165479.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8430..8930
                     /gene="rpsE"
                     /locus_tag="IVZ67_001398"
     CDS             8430..8930
                     /gene="rpsE"
                     /locus_tag="IVZ67_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8165480.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8945..9124
                     /gene="rpmD"
                     /locus_tag="IVZ67_001399"
     CDS             8945..9124
                     /gene="rpmD"
                     /locus_tag="IVZ67_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8165481.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9168..9608
                     /gene="rplO"
                     /locus_tag="IVZ67_001400"
     CDS             9168..9608
                     /gene="rplO"
                     /locus_tag="IVZ67_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8165482.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9608..10903
                     /gene="secY"
                     /locus_tag="IVZ67_001401"
     CDS             9608..10903
                     /gene="secY"
                     /locus_tag="IVZ67_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8165483.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10965..11612
                     /locus_tag="IVZ67_001402"
     CDS             10965..11612
                     /locus_tag="IVZ67_001402"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8165484.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11803..12021
                     /gene="infA"
                     /locus_tag="IVZ67_001403"
     CDS             11803..12021
                     /gene="infA"
                     /locus_tag="IVZ67_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8165485.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVZ67_001404"
     CDS             12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVZ67_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8165486.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12188..12553
                     /gene="rpsM"
                     /locus_tag="IVZ67_001405"
     CDS             12188..12553
                     /gene="rpsM"
                     /locus_tag="IVZ67_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8165487.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12581..12970
                     /gene="rpsK"
                     /locus_tag="IVZ67_001406"
     CDS             12581..12970
                     /gene="rpsK"
                     /locus_tag="IVZ67_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8165488.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13050..13988
                     /locus_tag="IVZ67_001407"
     CDS             13050..13988
                     /locus_tag="IVZ67_001407"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8165489.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14016..14396
                     /gene="rplQ"
                     /locus_tag="IVZ67_001408"
     CDS             14016..14396
                     /gene="rplQ"
                     /locus_tag="IVZ67_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8165490.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14668..15507
                     /locus_tag="IVZ67_001409"
     CDS             14668..15507
                     /locus_tag="IVZ67_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8165491.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15483..16352
                     /locus_tag="IVZ67_001410"
     CDS             15483..16352
                     /locus_tag="IVZ67_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8165492.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16342..17139
                     /locus_tag="IVZ67_001411"
     CDS             16342..17139
                     /locus_tag="IVZ67_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8165493.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17342..18088
                     /gene="truA"
                     /locus_tag="IVZ67_001412"
     CDS             17342..18088
                     /gene="truA"
                     /locus_tag="IVZ67_001412"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8165494.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18753..18990
                     /locus_tag="IVZ67_001413"
                     /pseudo
     CDS             18753..18990
                     /locus_tag="IVZ67_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19134..19910)
                     /locus_tag="IVZ67_001414"
     CDS             complement(19134..19910)
                     /locus_tag="IVZ67_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8165495.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            20033..>20240
                     /locus_tag="IVZ67_001415"
     CDS             20033..>20240
                     /locus_tag="IVZ67_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8165496.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLI"
BASE COUNT         6747 a         3620 c         4339 g         5534 t
ORIGIN      
        1 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
       61 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
      121 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
      181 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
      241 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
      301 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
      361 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
      421 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
      481 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
      541 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
      601 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
      661 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
      721 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
      781 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
      841 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
      901 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
      961 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
     1021 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
     1081 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
     1141 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
     1201 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
     1261 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
     1321 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
     1381 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
     1441 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
     1501 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
     1561 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
     1621 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
     1681 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
     1741 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
     1801 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
     1861 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
     1921 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
     1981 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
     2041 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
     2101 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
     2161 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
     2221 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
     2281 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
     2341 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
     2401 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
     2461 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
     2521 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
     2581 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
     2641 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
     2701 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
     2761 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
     2821 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
     2881 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
     2941 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
     3001 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
     3061 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
     3121 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
     3181 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
     3241 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
     3301 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
     3361 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
     3421 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
     3481 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
     3541 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
     3601 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
     3661 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
     3721 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
     3781 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
     3841 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
     3901 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
     3961 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
     4021 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
     4081 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
     4141 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
     4201 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
     4261 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
     4321 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
     4381 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
     4441 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
     4501 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
     4561 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
     4621 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
     4681 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
     4741 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
     4801 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
     4861 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
     4921 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
     4981 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
     5041 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
     5101 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
     5161 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
     5221 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
     5281 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
     5341 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
     5401 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
     5461 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
     5521 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
     5581 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
     5641 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
     5701 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
     5761 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
     5821 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
     5881 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
     5941 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
     6001 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
     6061 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
     6121 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
     6181 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
     6241 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
     6301 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
     6361 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
     6421 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
     6481 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
     6541 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
     6601 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
     6661 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
     6721 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
     6781 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
     6841 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
     6901 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
     6961 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
     7021 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
     7081 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
     7141 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
     7201 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
     7261 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
     7321 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
     7381 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
     7441 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
     7501 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
     7561 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
     7621 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
     7681 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
     7741 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
     7801 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
     7861 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
     7921 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
     7981 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
     8041 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
     8101 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
     8161 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
     8221 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
     8281 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
     8341 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
     8401 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
     8461 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
     8521 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
     8581 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
     8641 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
     8701 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
     8761 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
     8821 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
     8881 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
     8941 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
     9001 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
     9061 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
     9121 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
     9181 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
     9241 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
     9301 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
     9361 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
     9421 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
     9481 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
     9541 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
     9601 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
     9661 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
     9721 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
     9781 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
     9841 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
     9901 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
     9961 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    10021 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    10081 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    10141 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    10201 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    10261 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    10321 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    10381 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    10441 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    10501 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    10561 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    10621 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    10681 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    10741 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    10801 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    10861 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    10921 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    10981 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    11041 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    11101 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    11161 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    11221 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    11281 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    11341 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    11401 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    11461 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    11521 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    11581 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    11641 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    11701 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    11761 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    11821 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    11881 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    11941 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    12001 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    12061 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    12121 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    12181 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    12241 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    12301 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    12361 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    12421 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    12481 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    12541 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    12601 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    12661 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    12721 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    12781 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    12841 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    12901 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    12961 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    13021 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    13081 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    13141 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    13201 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    13261 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    13321 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    13381 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    13441 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    13501 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    13561 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    13621 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    13681 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    13741 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    13801 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    13861 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    13921 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    13981 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    14041 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    14101 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    14161 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    14221 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    14281 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    14341 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    14401 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    14461 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    14521 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    14581 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    14641 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    14701 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    14761 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    14821 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    14881 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    14941 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    15001 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    15061 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    15121 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    15181 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    15241 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    15301 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    15361 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    15421 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    15481 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    15541 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    15601 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    15661 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    15721 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    15781 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    15841 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    15901 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    15961 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    16021 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    16081 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    16141 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    16201 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    16261 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    16321 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    16381 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    16441 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    16501 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    16561 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    16621 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    16681 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    16741 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    16801 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    16861 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    16921 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    16981 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    17041 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    17101 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    17161 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    17221 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    17281 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    17341 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    17401 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    17461 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    17521 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    17581 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    17641 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    17701 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    17761 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    17821 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    17881 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    17941 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    18001 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    18061 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    18121 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    18181 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    18241 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    18301 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    18361 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    18421 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    18481 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    18541 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    18601 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    18661 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    18721 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    18781 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    18841 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    18901 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    18961 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    19021 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    19081 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    19141 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    19201 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    19261 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    19321 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    19381 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    19441 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    19501 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    19561 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    19621 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    19681 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    19741 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    19801 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    19861 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    19921 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    19981 aacaaaaaaa gtcttttact atttttactt aatttatgaa aggttgttta ccatgttaga
    20041 ccaaacagat ataaatatca ttgaggaact atcaaaaaat agtcgaatca caatgaaaga
    20101 acttggagaa aaagtacatt tgactggacc cgctgtctcg gcgagagtga caaaactaga
    20161 ggaaagtgga gttattgaaa attatacagt aaaattaaat atggaaaagc tagggttttc
    20221 gattcacgct tttttaatta
//
LOCUS       DACCZY010000047        20211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCZY010000047 DACCZY010000000
VERSION     DACCZY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.047"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            56..3445
                     /locus_tag="IVZ67_001416"
     CDS             56..3445
                     /locus_tag="IVZ67_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8165497.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3448..4869
                     /locus_tag="IVZ67_001417"
     CDS             3448..4869
                     /locus_tag="IVZ67_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8165498.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4866..6743
                     /locus_tag="IVZ67_001418"
     CDS             4866..6743
                     /locus_tag="IVZ67_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8165499.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6835..7677
                     /locus_tag="IVZ67_001419"
     CDS             6835..7677
                     /locus_tag="IVZ67_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8165500.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7829..8704)
                     /locus_tag="IVZ67_001420"
     CDS             complement(7829..8704)
                     /locus_tag="IVZ67_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8165501.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8911..9942
                     /locus_tag="IVZ67_001421"
     CDS             8911..9942
                     /locus_tag="IVZ67_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8165502.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10031..10336
                     /locus_tag="IVZ67_001422"
     CDS             10031..10336
                     /locus_tag="IVZ67_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8165503.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10515..12530
                     /locus_tag="IVZ67_001423"
     CDS             10515..12530
                     /locus_tag="IVZ67_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8165504.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12532..12816
                     /locus_tag="IVZ67_001424"
     CDS             12532..12816
                     /locus_tag="IVZ67_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165505.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12842..14215
                     /locus_tag="IVZ67_001425"
     CDS             12842..14215
                     /locus_tag="IVZ67_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8165506.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14298..16313)
                     /locus_tag="IVZ67_001426"
     CDS             complement(14298..16313)
                     /locus_tag="IVZ67_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8165507.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16335..17549)
                     /locus_tag="IVZ67_001427"
     CDS             complement(16335..17549)
                     /locus_tag="IVZ67_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8165508.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17770..18714
                     /locus_tag="IVZ67_001428"
     CDS             17770..18714
                     /locus_tag="IVZ67_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165509.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19010..19954
                     /locus_tag="IVZ67_001429"
     CDS             19010..19954
                     /locus_tag="IVZ67_001429"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8165510.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6898 a         3714 c         3856 g         5743 t
ORIGIN      
        1 ttaataattt tcactattca cgtcaaaaca gaaaaaaaga aaaaggaggg gaaaaatgac
       61 gaccacaggg aagaaactga aagttatttt catgctgata atattgagtt tatcaaactt
      121 tgtgccatta tctgcaatag cagacactac agatgatcca acagttttag aaacaatttc
      181 agctgaagtc atttcggatc agtctggaaa aaaagcactg aacatcaagc taaatgcgaa
      241 taacaccagt gctgaaaaga tagaaaaaga aattggtcta gtcgaaaatt acttaagtga
      301 tgtggaaaga aaagaaggag atggctatgc ttatcaggta aatagcggga aaattacgtt
      361 ggaaatctca tcaaacacta aacaaactat cgatctgagt tttccaatcg atccagcact
      421 ttaccacagc caggcaaaca agctgatcgt cgataataaa gaatatgaca ttattgatga
      481 gacagaaaat aagaaagata cagatgtgtc agtaccaaag ccagacgaaa tagaagaaga
      541 atcatcaaaa gaaaacgaaa attctgtcag cccatttaca ttgcctacat tatccttgcc
      601 agctgtgagt gtgccatcta atcaaacgat tcctacagaa tatacaacag atgatcaggg
      661 cacttatcct aaagccagtt ggcaacctac aggaaataca aatgttcttg atcatcaagg
      721 caataaaaac ggaacaaatc aatgggatgg tataaattct tggaatggag atcctaatga
      781 tcggacccat tcgtatatcg aatatggagg aaccggtaat caagcagact atgcgatacg
      841 aaagtatgca aaggaaacaa gtacacccgg attgtttgat gtttatttga atgctcgtgg
      901 aaatgtacaa aaagatatca cgcctcttga tctcgtattg gtcgtagact ggtcaggaag
      961 tatgaacgac aataatcgga tcggtgaagt aaagattggt gtcgatcgtt ttgtcgatac
     1021 tttagcagat agcggtatca cagacaaaat caatatggga tatgtcggct actcaagcga
     1081 aggatatagc tacagtaacg gtgcagtaca gatgggttca tttgattcag tgaaaaatca
     1141 agtaaaatcc attacacctt cacggacaaa tggtggtact tttacacaaa aagcactaag
     1201 agatgcagga agcatgctat ccgttccaaa tggacataaa aaagtgatcg ttttgctgac
     1261 ggatggtgta ccaacatttt cctataaagt acagcgggta cacgcacaat caagcagcaa
     1321 ttattacgga actcagtttt ctaatacgca agatcggccg ggaaatactt ctctaatctc
     1381 aagaatctat gatgcacctg accaaaacaa tctatccaga agaatcgaca gtacgtttat
     1441 cgcaaccatc ggagaagcga tggcactcaa agaacgagga atcgaaatac atggtcttgg
     1501 catccaactt caaagcgatc cggcagctgg tctctcaaaa gcagaagtag agtctcgtat
     1561 gcgacaaatg gtttcatcag atgaaaaagg cgatctttac tatgaatcag ctgatcatgc
     1621 aacagatatc tctgaatacc tagccaaaaa agctgtacag atctcagcaa ctgtaagcaa
     1681 tggacaaata aatgatccaa tcgcagaacc attcatttat cagcctggta cactttcagt
     1741 caagagtgtg gggacaagtc ctacaacggt cactccatct atttccatag aaggaaatac
     1801 catcaagagc aatcagatct atttaggaaa agaccaagaa atccaaatcc attaccaagt
     1861 gagaatccaa acagaaaatg aggacttcca tccaaatttc tggtatcaaa tgaacggcag
     1921 gacaactttc cagccaaaca ttgataccaa tgaattagct gaattcggta taccatctgc
     1981 taaagctccc ggagtcagtc ttcacatcaa aaagttatgg gaagaatttg acaacaatct
     2041 agctgatcgt ccagatcaag ttacttttga gattcaacgg gaacatacga caaatgctgc
     2101 agcttggaaa aacggatata ttcgaatcat taaaccagct aaagatacaa caaatacgtg
     2161 ggaacgtgca gacattgaca aattatctgc aaatagcgga gaaagttatc aagagatatt
     2221 atcactacct caatacaata atcaaggtca agcattcagt taccaaacaa tcaaagaatt
     2281 acctgtacca ggatacgatt ctcaacaaat agatgcaatg acatggaaaa atactaaaca
     2341 attcacaccg ttaaacttga aaataacgaa aaattcctct acaggtgaaa aggatcttat
     2401 tggcgctgtt ttcaaattaa caggagattc tattgatact ttactaacag atcatggcga
     2461 cggaacctat tctcttccag aaaatgtcaa attgcaaaaa gaaatgacct atacgctgac
     2521 agaaacaaaa gctccagaag ggcatggatt aagcaaaaag actacttggg aaatcaagat
     2581 cgcttctgat ggtacggtaa ccattgatgg aaaaacagtc actacttccg atgatacgat
     2641 ccagttgact attgaaaatc cttttgttga agttcctgta gcagtacgta agtatgcgat
     2701 gcaagggacg gacaaagaga taaatcttaa aggagcagca ttttccctac agaaaaaaga
     2761 agcaaatggt acttatcagc caattgacag ccaaacaacg aatgaaaaag gtcttgccag
     2821 ttttgattca ctcacacctg gtaaatatcg agtcgttgaa acagctggtc ctgccggata
     2881 tgatacttcg ccgggaaatt atgaattcca aatcgataaa tatggaaaaa tcatttacac
     2941 gggaaaaaat accgagatga caaataatgt atggacgctc actcatcaaa atcgactaaa
     3001 agcgtttgat ctaacggtac acaaaaaaga agacaacgga cagacattaa aaggagcaaa
     3061 attcagactg cagggaccag aaatggactt agaatcgcca aaagatggac aagaaacaga
     3121 tacctttcta ttcgaaaatt taaaacctgg aacttatacg ctgaccgaaa cttttacacc
     3181 agaaggatac caaggtctaa aagagccagt tactatagtt atacacgaag atgggtcaat
     3241 tcaagtggat ggacaagatc atgaatctgt tctgtcacca ggagccaaaa acaaccagat
     3301 ttctttagac atcacgaatc aggcaaaagt accattacct gaaacgggag gaattggccg
     3361 tttaggaatc tatctagtag ggatgattgg ttgtgcgttt tctatttggt atcttttttt
     3421 gaaaaaagaa agagggggca gctaaatatg aaaaaacttg gttggcttag tatgtgtctc
     3481 ttcttgttac tatttaaacc agcttttact caggtagcaa cagaaacaga aacagaaatg
     3541 gttcagatta ctttacacaa attgcttttc ccaaacgggc aactgccgaa aaatcatcca
     3601 aatgacggac aagaaaaagc tttattacaa acgtatcgag gattaaatgg tgtcacattc
     3661 caagtttatg atgtcacaga ttctttttac catctacggg aaaagggcaa aacggtagaa
     3721 gaagcacaag cagagatcgc aaaaaacggt gcgtcttccg gtatgtttac cgcagaagca
     3781 acaactacaa ctcttaacaa cgaagatggt atcgcttctt tttctctggc cgctaaagat
     3841 caagaaaaaa gagataaagc gtatcttttc attgaatcca aagtaccaga agtcgtcaaa
     3901 gaaaaggcag agaatatggt agttgttctt cctgtacatg gacaaaacaa tcaaaaactt
     3961 tcaactatcc atttgtatcc taaaaatgaa gaaaacgact accctgatcc accttttgag
     4021 aaggtattag aagagcctag aaatgatttt acgattggtg aaaaaatcac ttattccttg
     4081 catacgacaa ttcctgtaaa tatccttgac tatcaaaagt tcgaattgtc agatagtgcg
     4141 gatgaagcat taacgttttt acctaatagt ttaacgattt catcgaatgg agaaaagctg
     4201 acagaaggct ttgtcataca caagaaacct cacggatttg atgttttatt ttcgatccct
     4261 tcgttggaaa aatatgctgg aaaaaaactg accatttctt atcagatgca gctaagcagt
     4321 acagcacagg cgaacaagga aatcaacaac aacggaacac tggattttgg ttttggtgtc
     4381 agtacaaaga aagtctctgt atatacaggg agtaagcaat ttgtcaaaat cgagacaaat
     4441 aaaccagata aacgattagc tggcgcagta ttccttatta aaaacaaagc aggaaattac
     4501 ctccagcaaa cagccaacgg atacaagtgg acaaagaacg aatcagatgc gcttcacctg
     4561 atttccgata aaaatggcgc tttttcaatt tccgggttga aaacaggaag ttatcgatta
     4621 aaagagatcg aagcaccttc tggttatatt ttaagtgaaa cagaaattcc gtttaccatt
     4681 tcaacttttc tttctgagga taaagaggcg gacagtatat tgaaagtagt caataaaaaa
     4741 gaaaatagcc gtccatttct tccaaaaaca aacgaaacga aaaatacact tttaggcgtt
     4801 gttggtatgg tattcgcaag ctttgcaatc tggttgttta tcaaaaaaag aacaggagtg
     4861 aaaaaatgaa aaatcataaa aaaataaacg ttatgttagg agtccttttc cttattttac
     4921 cattactcac aaacagcttc ggcgcaaaaa aagtgtttgc agaggagaca gcagctcaag
     4981 tcatccttca taaaaagaaa atgactgatt tacccgatcc tttaatccaa aacagcggga
     5041 aagaaatgag cgaattcgat caataccaag gattagccga tatttcattt tcagtttata
     5101 acgtcactca agaattttat gcgcaacgag ataaaggagc gtccgtggat gcagcaaaac
     5161 aagcagtcca gtctttgact cctggtacac cagttgcttc aggaacgaca gatgctgatg
     5221 gaaatgtcac tttatcttta cctaaaaaac aaaatgggaa agatgcagtc tacacgatca
     5281 aagaagaacc aaaagacgga gtgtcagctg ccgcaaacat ggttttagct ttccctgtat
     5341 atgagatgat caaacaagca gatggctctt ataaatacgg gacagaagaa ctagatacta
     5401 tccatctcta ccctaaaaat acagtcggta atgatggaac gttgaaagtt acaaaaatcg
     5461 gtactgccga aaacgaagca ctaaatggag cagaatttat tatttctaaa gaagaaggaa
     5521 caccaagcgt caaaaaatac atccaaagtg tcacagatgg attgtacact tggacaactg
     5581 atcaaaccaa agccaaacat ttcattactg gtcattctta tgacatcggc aacaatgact
     5641 ttgccgaggc atctattgaa aaaggccagt tgatcgttaa tcatttagaa gttggaaaat
     5701 ataatttaga agaagtaaaa gctcctgata atgcggaaat gattgaaaag caaacaatca
     5761 cgccttttga gatcctggca aatagccaaa caccagtaga aaagaccatc aaaaatgata
     5821 cgtctaaagt tgataaaaca acacctcaat tgaatggaaa agatgtcgca atcggtgaaa
     5881 aaattcaata tgagatttct gtcaatatcc cattaggtat cgctgataaa gaaggaacgc
     5941 aaaacaagta cacaacattc aaacttatcg atactcatga cgctgcttta acatttgata
     6001 atgattcttc aggaacgtat gcttatgcct tatatgatgg aaataaagaa atcgacccag
     6061 taaattattc tgtcactgag caaacagacg gattcacggt ttcagttgat ccgaattata
     6121 ttccttcatt aactcctggc ggtacattga aattcgttta ctatatgcat ttgaacgaaa
     6181 aagcagatcc aaccaaagga ttttctaacc aagcaaatgt cgataacggg catacaaatg
     6241 atcaaacacc accgtcagtc gatgtcgtta ctgggggcaa acgatttgtt aaagtagatg
     6301 gtgacgttac atcagaccaa acacttgctg gagcagaatt cgtcgttcgt gatcaagata
     6361 gtgacacagc gaaatattta tcgatcgacc catccacaaa agccgtcagc tgggtatcgg
     6421 cgaaagaatc agcaacggtt tttacaacca caagtaacgg tttaatcgat gtgacaggtc
     6481 taaaatatgg cacgtactat ctggaagaaa cgaaagcgcc agaaaaatat gttccattaa
     6541 caaaccgtgt agcatttact atcgatgaac aatcttatgt aacagcagga cagttgattt
     6601 ctcctgaaaa aataccaaat aaacacaaag gtacacttcc ttcaacaggc ggtaagggaa
     6661 tctatgtgta tatcggtgca ggagtagtcc ttctactgat tgctggactg tactttgcta
     6721 gacgcaagca cagtcagatt tagtcttttt gcaagaattg gaaaaaggag cagcggatta
     6781 cgcaaatgct cctttttctt ttattatcaa acagtcatca caaggagaac acaaatgaaa
     6841 ccaaagaaaa ggaaacttct agatctgctg atgcttttct tgcttctctc aggaataggg
     6901 atactagctt atccatttgt tagcgacgcg ctgaataatt atctggatca gcagatcatc
     6961 tctcattatc agcaacaagc ggtcaaagaa aatgaagaag tcatggctaa aatccaagaa
     7021 aacatgacaa agaaaaacca gcaattagca aaagaaggtg gaaatccagg tgcagatccc
     7081 tttactaaga aaaaagaacc tgcgaaaaag gatagaacct attttgagaa acatacgatc
     7141 ggtattttga cgatccctga gatcaatgtc catttgccaa tttttgatga aacgaatact
     7201 gtgctgctag aaaaaggtgc ctcattattg gaaggaacgt cttatcctac ggggggagaa
     7261 aatacacatg ccgttatttc cagccatcga ggactgcctc aagcaaagtt gttcacagac
     7321 ttaccgaaac tgactttagg tgattctttc tatatcgaaa tcaatggacg gacactcgct
     7381 tatcaagtcg atcagatcaa gacggtagaa ccgactgata caaaagatct gagaatcgaa
     7441 aaagggcagg atttcgtgac actcctaact tgtacacctt atatggtcaa cagtcatcgc
     7501 ttacttgtca gagggcaccg ggttccttat catgcaaaag aagttcaaaa agaagtacag
     7561 aaagtatccg agaacaaacg atttttcctg tatggattgt tagctgcatg tatactgatt
     7621 ttcctccttc ttatatatct ttttcgtcaa tcgttcaaaa taagaaacag aagttagcta
     7681 ttcctcattt ctataatata tctattcgaa tcgttccgta aacaaacagg ctgaggtttg
     7741 aaaaagaaaa cttcagcctg tttttatgat gatatcaaag agagcctatc aaacactatc
     7801 tttcttatta ctaaatgagg atcttttctt aagttttccg atattcttta agaaggcctt
     7861 tttgcatcaa ttcttgtttg tttttcaagt tgcggatatt ctcttcaaaa ttttgagaat
     7921 atatcactga gaataacgta tttaatacgt aatcgaaggc aatttgagag gagaaagtcg
     7981 ctactttagc aaaatcatat tcctcctgcg atataacaag acacatatca gcctgtttgg
     8041 ccatttctga atgctgattt ccggtaatca gcaaggtagg ggcctctaca tgcttcaaat
     8101 aggtcattat tcgttcataa tgatggatgc gaccgctgta gctaataaaa atggcgcaat
     8161 cctcgctagt caaattggct gcattccaag aagaatcact gtattcttca gccatgatca
     8221 aaaacttatt caacttgacc attttattct ggaatttcct tgccgtgatt tgagaatcac
     8281 cttttgcaaa aagaaagatt cgctgtgcct tgttcaatac tttaccgatt tgatctaaat
     8341 cttcatgttc aatctgcaat tgtgcttttt ttattgactg gatcgataaa tccgccattt
     8401 tctttgcaat ttgctgagtg ctatcttcac ttgaaaaagg aaaattagga tcaatcgttt
     8461 gattgaactg agcaatcaat tgatgaactt cccgccccag ctcgaaacgg aattctttaa
     8521 atccgctgaa tcccattttt ttgcataaac gaatgatagc tgaatgggaa gtatacgtag
     8581 cttttgctaa ttcttcaatc gtcatgtaga ctgcagcttc gatattccta cggatgtaat
     8641 cgacaatccg tttctctgtc gtggtaaaca actcttgttt tgttagtttt tcttcaataa
     8701 gcatgatctg acctccaaat tctatcttac tgataaacac ttggttccgc tactatcttt
     8761 ttgattttta tccttacttt gaacattaat ttcaccaaac cgaatgataa caagtgtttt
     8821 tctgaacttt tttaccagaa tagctattat aaatactttc tgcgcttttc taggatatac
     8881 tgaaaacatc aataaaagga ggcaccactc atgcttacaa tcgcttatct tggcaatgga
     8941 aagagtacaa atcgctatca tttgcctttt tcaagaaaac ttacgaacaa aatcaatatc
     9001 aaaaaaattt tttccccatc tgaaccagtg tggaaaacat tcgaagacat cgaatatact
     9061 gatcagctgt ctgatctttg ggatgatcct gagatccaat tagttgtcat cactacacct
     9121 agtcaatttc attatgaata tggaaaaatg gcactcgaac atggtaagaa tgtactagta
     9181 gagaaacctt ttgcagaaac gtcaaaggaa gcagaagagt tatttgcatt agcaaaagaa
     9241 aaaggtctat ttatccaatg ttaccaaaac agacggttcg attctgactt cttgacagtc
     9301 caaaaagtaa tcgaaagcgg aaaattaggg gatattttag aagtagaaat gcattatgat
     9361 tatttcagac cggaagtacc agaaaatacg catgaatttt ctactgcgaa cagctattta
     9421 tatggacatg cttgtcatac agtagatcaa gttctttctt atttcggcga accagatgat
     9481 atcagctatg atgtacgtca acttttaggg aaaggacgaa tgaatgatta cttcgatctg
     9541 gattttttct atggaaatat gaaaatttcg ataaaatcca gttatttccg catcaaagaa
     9601 cgtccaagtt tcgtcgttta cggaaaaaaa gggatgttcg tgaaacaaac aaaagatcga
     9661 caagaagaac atctgaaaat gttttacatg cctgatcatg aagactttgg tatcgatttg
     9721 cccgaacatt atggtgtatt gacctatata gatgaacaag ggagttatca tgaagaaaaa
     9781 gtaatttccg aggttggtga ttacagccgt gtctacgaag ggatttacga gacattagtt
     9841 aatggcgcag aaaaagtcat caaagatgaa gaaacaatct tacaaatgaa aattttggaa
     9901 gaaggaatca aaagcattca agaatcagaa ggaaaaatat gacatatttg taagaatcgg
     9961 gtttgttttt tgacgcataa actgtcttat tgtacgatga aaaagtatag aactactagg
    10021 aggaaccaac atgacaaatt atgacggtga aaaacacaaa cgattaagag gttcaatggc
    10081 ccctaaaaca gaagaagaag tggctttagc aggaactaat tctggtgaaa cagaagacaa
    10141 agaagaatca ttagctggca caaactctgg tgaaacagaa aataaagaaa cagctttatc
    10201 tggttcaaat tctgtagaag aggaaacaaa gcctaatgag cccaatcact ttgaaaaaga
    10261 tatcgaaaaa gcggttgaag atgtcaaaga aaaatttgaa gaactgacct caaaaaataa
    10321 acatgatcat cattgatcgg acgaaatcaa ttcataaaag caggagacat ttggtcacct
    10381 gctttttttc tgtatccatt cgatagaaat cgttcggtac agtcgtaaaa aaaatccaac
    10441 tgtaagttgt accaaacacc cctcgattta aaagcgctac catactagaa tataagtagg
    10501 aggtgaacag cgctgtgaac tacaaaatta gttatctcct gaaaacacca agagaaatca
    10561 gtctagaaaa agcagcagag atcatcgaag taaatacaag cgaggcgatc aaacaattaa
    10621 ctccttttgc cgaatatgtg gatgataccc gtatccgtat aaagaaattc cgagcaatcg
    10681 actgggcaga aaaatttttg gatattactc gatttgaagc tgtgtatacc gaacaagagc
    10741 gaagagatct tctttatttg atggtttatt cgactgcaac gtctgtttct gtttttcatt
    10801 ttcaagattt tcttcaggtt tctaaaggga cagtcctgtc agatatcaaa aagataagaa
    10861 atgaattaga agtggaagat tgtcagctgt cgtatactag gaaaactggt tttaggctgc
    10921 tagggtcaga agcaatatta agaagagaag caaaaaatcg cactgctcgt ttgatgcagt
    10981 cacttgctgg aagatttggg ctcacgtatt taaccgcaac cctttccatt gattattacg
    11041 ccaaagttag agatttgcta gataaaacga tttcactatc agatcttcga ttcgcaccaa
    11101 atcgaatgga tgaattagct tattatttga gtttgacaac cattcgaatt ggaaaaacat
    11161 ccatggaact ggtaaaagat gctgaactgt tggcttctct tgcagtcact gacattagtc
    11221 gacaagtatt gactggcctt ggctattccg aacatcaaaa ttcggagatt atatttttct
    11281 ctctttgctt agcatcgatc gttgaaggag atgtccagga gcctgcactt gattttttgc
    11341 tggaatgttc ttcagaagtc atccatcgca tggaattcct gatggccgta gaatttgctt
    11401 cgtttcgtga attattgatg aatgtctttt atcacttggt ccctgcctat tttcgtattt
    11461 cctattcttt ttatttacct aatgtgatga tcgatcaaat taagcatcaa tatgcttcca
    11521 tctacgaaat gacaagaaaa gcattacttc cgttagaaag acgaataggg aagagcatcc
    11581 ctgaagaaga gattggtttt ttcactatac tatttggcgg agagatcaga aaagcggacg
    11641 cagaagaaag aaatagaaaa ataagagccg tgatcgtctg tccaagtggc attagctctt
    11701 cattgatctt aaaaagtgaa cttcagcaat tattcccgat gatctttttt acagagacta
    11761 attcagctga ccgcttacac gaggttgacg aaacttctta cgatattatt ttttctactg
    11821 tacctctcac acttactgat aagaaaaagt ctctctatgt tctgcatcca atcatgactg
    11881 acttggaaaa aaatcagctg atcaatcaag tacaaagaga ttggctgata ccaggatttt
    11941 caatgccttc tgcccaagaa ttgttgtcgg cgctactgcc ttatatcgaa ttaaaagaag
    12001 gcgtagacga agaaaagctc tatcaagtat taaatcgtaa aatgaacaaa cttttagaaa
    12061 agaaggagga tcaacgtcct atgctatctg aattattaac agaagaaatg atccaattat
    12121 cggatcaacc aaaaaactgg caagaagcaa tcacatttgc cgcacagccg ctattatcag
    12181 aaaataaaat agaagaaagt tatatcaaag ctatgatcga gcgcgtggag cagtatggtg
    12241 cgtttatcca tattggcgat cacatcgcat tacctcatgc tcgtccagaa gatggcgtaa
    12301 acgaagtagg gatgtctttg ctgaaacttc aacaacctgt ggatctgctt gataatccag
    12361 cccatcccat tagtttgttc atctgtttag ctgctatcga taatgaggca catcttcggg
    12421 ctttggcaaa tttaacaaag ctactttcta ataaacaaaa tcttcaagat ttattacacg
    12481 catcaacaaa agaagaaata ttagcgatca taagaaaagg agacgaatga tatgaaaatt
    12541 tttgcagtat gtcaaagtgg attaggaaca agttttatgg tagagatgaa catcaaacaa
    12601 gcacttgcag atcttggagc agatgtggac gagtttgaag taagtcatac ggatgttgga
    12661 agtgtatcta gcgatatggc tgattatttc ttcattgagc attctttgaa agatacgcta
    12721 acttctatac ctgatgaaaa gctcgtacct ttgcaatcga tcattgacag cgaagaagta
    12781 aaagaaaaag taacgaagat cttgtcaaaa gaataaaaat gattcaaggg aggaactatc
    12841 aatggataaa ttgttgaatt tgtttgtgtc aatcgtcagt cagccctcca tcctcgtttc
    12901 tttgattgca ctgcttggtc tcgttcttca gaagaagaaa ttttcagaag tgatccaagg
    12961 aactatcaaa acatttgtcg gattcctagt actgatcggc ggtgctggtt taatcagtaa
    13021 ttcactaacc ccttttgctt cgatgttcca aacagctctg catactcaag gagtagttcc
    13081 tagcaatgaa gcagtagtgg cgattgcttt gcaacaatac ggtacaccta ctgctttgat
    13141 catgctagta gggatgatcg tgaatatttt acttgctaga ttcacaagat ataaattcat
    13201 ttttcttaca ggtcaagcaa tgctttatgt ttcttgtcta acagcagtca ttttagtaag
    13261 cgcggggttt actgcaaatt tgtggatgat cttattaggc ggggtcttcg aaggaacact
    13321 attaactatt acgcctgctc tttgtcaacc gtttatgaag aacatcactg gaaacaatca
    13381 agttgcgtta gggcataccg gaaatatcgg ttatgcttct agtggatgga tcggaaagtt
    13441 ttttggaaac aaagaccgtt ctacagagga cataaagatt cctaaaagtt tcagcttttt
    13501 gcgagattct actgtatcga tcatgctctt gatgtcgatc gtatatgtcg cacttgcttt
    13561 gttagctggt cctggatttg ttgaaagaga actaagcaat ggagacaacg ctgtaatctt
    13621 tgcactgatc caagctggaa cattcacagc tgggtttgtt atcgtccttc aaggggttcg
    13681 aatggtgttg gcagaaatcg ttccagcatt ccaagggatt gctaaaaaac tcgtacccga
    13741 ttctaaacca gcattagatg ttcctatcgt ttttccatat gcacctaatg ctgtattaat
    13801 tggctttttt gttagcttct ttgtaggaac actatccatg tttgctatga taggcttgca
    13861 aactacagtt atcattcctg gggtagtagg acatttcttc tgcggagctg cttctggtgt
    13921 cttcggcaac tcgactggcg gtagaagagg ggcggtatta ggttctgctt tcaatagctt
    13981 attgattagt tggctgccat tatttatcct tccagtatta ggagatctta aaatcgcttc
    14041 ttctacattt gccgatactg attatctagt accaggactt gtacttggga aactaggaga
    14101 atacggtcaa atagccttga cagttggaat catcgctttc tttttacttg tcgtattatc
    14161 tagctttctt tttgccggaa aaagaacaaa cattgtttct aaagaacaga attaagcaaa
    14221 cttcaaaata aaaattgctt ggacaatatc agaagaagat ttaaagtctt ctgatatgtc
    14281 gcaagcagtt ttttgtttta tgcatttatt tttgaatagc ttgttaagac aagtggctgt
    14341 tttgctagaa gaggataact tttaccattc attttcactg ttccgcctgt tctatcagaa
    14401 gtaatcacga tcttatcttt tgttttttca agcgaaatct ttccatcttt gataggtagc
    14461 gtacaattca gtgtttcaaa tgaatctaga ttaggttgta tcataaattc ttcataacct
    14521 ggctttgttg gatacaaacc gataaaatag cgtcctaaaa gatagattgg gcttgctccc
    14581 catgcatggc ataagctttt tccataagga tctccataca tactgtactt ttcatttcct
    14641 gtttgagtag ggtcgtattc ttcccagaaa gttaccgcat catgagataa catccctccc
    14701 caataatcaa gaatcgtttg gtagacgata ttcgtctgtc cgagtttgca taaagcatct
    14761 tgttcataga atttgaaata tggagtagta atctgtgtga tagtgtcatt caataagaca
    14821 ttctttaaga tcgattgctg ttggtctttg ttagcaaaat cgaataaaac agcgaaaata
    14881 ttcgcatgac gcgtcacatt tcttctccca gattcatagc aatcgataaa agcgccaagt
    14941 tcttcatccc aaaagtagtc aaataggttt tgtttcaata gttgatattt ttcttgatag
    15001 agatgagtat gataattttc gttctctcca tatgtttcag ctaagatatc tccgcaagtg
    15061 atcatggaat gataggcttt cagtaataag acttgttctg cagcaagcgt tccttcttta
    15121 tccatttcag accaatcaat aaatatccaa tcttttgctc tcccataaat aaaccctaat
    15181 tcattcgttt gctccatcaa gtatctcatt aatgattgca tcttagggta gacgatgcgt
    15241 aaaaattcat agtctttcgt catcatatag tggttttcga taccaatcaa ccacaagatc
    15301 gaatagtcaa cgatagtatt catatgctgc ttgatttcat tttgtcctct taatgctaat
    15361 atcgttcgtt tattgatttc ctcgtcaaaa aaaagatatt gattgatcat gaatgattga
    15421 tacgcatctc cagaccaaat ccagcgatct cgtttgatac cgtcgataaa aaacaaatca
    15481 ctacataact tgaaggcttc ttctgatact gaccagatct gattcagtaa agaagaatcg
    15541 cttgaaaaag cagcttgttt cgaaagagat aaaaattcat gaaaaacttg aagttgaacc
    15601 attccagtag aaacctctgg tataaacaaa tagcgaaaag cgcgtttacg agctttcatt
    15661 tttcttgtca ttcctatttg cttatagtag caataatcga tatccatagc ttctacatcg
    15721 gactctccat aacatagagt gatagattcg atatggtcat ccagagatat tagttctaca
    15781 cagccattca ctgctcgccc aaaatcgaat aagattcctc cgccaatctc cgtttcagtg
    15841 actggatgga cgcattcttt ttgataatag atttgatttg gatcttttgc gatatcgaca
    15901 taaagcggac tccagccagc tggatattct tcaataaaat tacttgctgt ccagccaata
    15961 tctgatttta cttcgtctcc ctcgataaac atcgctggta gccctgatgt atgtcctaca
    16021 aagatacgaa tttttgcttt accagctgga caagtcagcc attttccaca tggatgtttt
    16081 tgtccattaa tttctacata cccaactcct tggatcgtca ctttaaacat ggaaggttga
    16141 tcaagaaagt aatatctctt aaacttgacg ttccgatggc aatcatccat gtaccagtac
    16201 gctggccagt caaaacctcg ttcttcccgt tggaaatttt gtagcagtcc atggcggatc
    16261 tcaaagtctc caggatacca aagccatcta tctttatgtt tttcatttgt cattttacac
    16321 gacctccatc aaatttattt tgttgtcaat tgttctttta tttcttcatc tactcttatc
    16381 actgtctttc tattcgtcct gtgtactact agaaggctaa taagcgcgct aatcaacagc
    16441 aagatactgc cattgacgaa aacagcaagt gttttactcc caagagaagc aaaagagaga
    16501 aagagcaaac catatggtgc aatgaaagta cctaaattag cgccaaccaa taagattgaa
    16561 gtattgaaac tctccgcgta cttgggcgct tggatactta cttggttgaa taagtaagga
    16621 atgaataacg aaaaagaaat tcctgttagg atcgcaccaa acccaatcac atacaggttt
    16681 atcacagtgg caatcaatac aaagccgatt cccatcagta gtaaagaaag tgtaagcata
    16741 tagcgatgtg cttttgtctg aagccagcca aaacaaactc cggcagccat acttgccaat
    16801 tgcatgattg ttaagatatt cgctgcatta gtttcgtttc ctagagaata agtcgtcagg
    16861 actgtggcta atttaatcga tacagcatta tataccacga tgaccagcaa gagaaaaata
    16921 ccataactaa tcattttagg attcgttttg attacttgtc ttgttggggc tgtgccatgc
    16981 gattgaagtt cagttgtttc aggtgtagga acaaacaaaa caaacaagac aagaataggt
    17041 aagctgatgg cgtagactaa aaaggtatat tgccaaccga actgtactag ctgtcctgca
    17101 acaaatgtca ttaaagctgc accaaatcct tgaaatgcac tttgaaaacc aatcatagag
    17161 gctcgttcat ctccgtgata aaaggcagca atcatgctga ctgccaaaga attgatcaat
    17221 ccaaagccaa tacctagtcc agctcttgaa gctaagacaa tactgtaact ttgtgagaag
    17281 agggggagga taccacaaat cgatgcaatc actaatccga tgatcacagt ttgtttttgt
    17341 ccgattttcc tagcaataaa actactaatc aaaacaaaaa tagtgaccat aaaagaggga
    17401 gcagtcgtta ataattcaac agcagataaa gaacgcgtgg cataagtagc tgacatactg
    17461 ggaattgcag acgtgactgc accagcagaa gtcaaaataa gagagactga cagcagagaa
    17521 aatttcatca tctttttatt cataagcatg ttcctttcat ttttgtaagc gcttatcaaa
    17581 aatatagtat gaagtggtta ttctctcaat agcggaatca caggaagttc atgtcccaac
    17641 catagaaagt ctttatgaaa ccaaaaatag ctttgcatat aatagcagta cagcgtgaac
    17701 taataagcga aagaaaccgc ttacaaaaag gagggagtca attggaacta gctgaattag
    17761 acattttttt tgcgtaacga agaatcaatc gaaactctgc agcgtgaaca aggaatgaat
    17821 atcaatgatt taaacttagc ttaccatctt cagacaccgc caaaaatcca ccgaggggat
    17881 ctatttcaac agggaaatat tcatatcagt aaacaccgtc gatatgcgga agttcccgag
    17941 catacacatg attttgttga aatcaattat atgtatagcg gtacatgttt acaaattatt
    18001 aatggagaaa ggatagagat ggaagaaagt gacgtggtga tcattgataa agaagctact
    18061 caaagaatcg aatacacagg gcaagaggat attttaatca acatcttatt aaaagaagaa
    18121 accttatcta cagagatatt gaatcatttg gcaagcacaa ataatctact ttcttctttt
    18181 ttgataaatg catcaaaaga aaaaggggaa catacacaat tcattcattt tcgcggcgga
    18241 aaaacaagtc gtttgacgca tttgattgaa aatatgctgt tgatttattt tgatacatac
    18301 agtcataaga tccgttcgtt aaatctctat acatcactat tactgatcga attgactcat
    18361 ttattagaac aacaaagctt ttcatcagca gatggatgcg cagaaaatga gatgcttaga
    18421 gtattagatt atatcgataa gcattttact tcttgtacgt taaaagaaac agcagctcat
    18481 tttggctaca atccggtcta tcttagtaat aaattgaaaa tgtattttga tgcttccttt
    18541 caagaactgg ttctaaagaa aaaattcgac tttgcgttgg aactgattca agaaacgagt
    18601 ttttcgttgg aagaaatcgc taatcgtatc ggatataaac aaacgacttc tttattcaaa
    18661 ttattcaaga agtattcaac atgtactccc aatgaatgga gaaataaaca ttaagaagaa
    18721 aatctttcac taaagcatat gaaggaaaat tgttttaggt gcgattgctg tcttattata
    18781 tcgttgcagc gaattttttt ggatttattt tcagttctaa acttttggtt gtattatcag
    18841 gggatatagg tgtaaacatt tgtcatttat ctgttatgcg tattcaggaa ttcgtttcca
    18901 tcaaaaatta taataacaac tattataatt aacatgttga taaaatgaat aaacaagact
    18961 atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca tgaaaaaaac
    19021 aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg cttatgcaat
    19081 gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag aaaaagcaga
    19141 aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg tggctgtatg
    19201 gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag ctggtatcaa
    19261 ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa tcatgcgcca
    19321 aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg cttcaaaccc
    19381 agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat cacgtgttat
    19441 cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat tgaaattaaa
    19501 agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt cagaagttgc
    19561 tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag aaaaagacca
    19621 tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg ctgcttatga
    19681 gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg cacgtatcgt
    19741 aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt taactggtga
    19801 atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa atggtgttcg
    19861 tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca aatcaacaag
    19921 tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga actaaaaatc
    19981 ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata tctaataggt
    20041 aagcaaataa atagaatggt taaaattaat cggtccgaaa atgtatccgt acatatgaac
    20101 atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt atctgtggtt
    20161 aattaaaaaa tccttccgca acggcaaata caatgattca tatctaattc g
//
LOCUS       DACCZY010000048        20013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCZY010000048 DACCZY010000000
VERSION     DACCZY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.048"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            48..860
                     /locus_tag="IVZ67_001430"
     CDS             48..860
                     /locus_tag="IVZ67_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8165511.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            857..1657
                     /locus_tag="IVZ67_001431"
     CDS             857..1657
                     /locus_tag="IVZ67_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8165512.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1818..3086
                     /gene="rseP"
                     /locus_tag="IVZ67_001432"
     CDS             1818..3086
                     /gene="rseP"
                     /locus_tag="IVZ67_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8165513.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3154..4863
                     /locus_tag="IVZ67_001433"
     CDS             3154..4863
                     /locus_tag="IVZ67_001433"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8165514.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5075..9433
                     /locus_tag="IVZ67_001434"
     CDS             5075..9433
                     /locus_tag="IVZ67_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8165515.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9578..10051
                     /gene="rimP"
                     /locus_tag="IVZ67_001435"
     CDS             9578..10051
                     /gene="rimP"
                     /locus_tag="IVZ67_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8165516.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10074..11249
                     /gene="nusA"
                     /locus_tag="IVZ67_001436"
     CDS             10074..11249
                     /gene="nusA"
                     /locus_tag="IVZ67_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8165517.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11271..11564
                     /locus_tag="IVZ67_001437"
     CDS             11271..11564
                     /locus_tag="IVZ67_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8165518.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11561..11872
                     /locus_tag="IVZ67_001438"
     CDS             11561..11872
                     /locus_tag="IVZ67_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8165519.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11885..14191
                     /gene="infB"
                     /locus_tag="IVZ67_001439"
     CDS             11885..14191
                     /gene="infB"
                     /locus_tag="IVZ67_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8165520.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14218..14565
                     /gene="rbfA"
                     /locus_tag="IVZ67_001440"
     CDS             14218..14565
                     /gene="rbfA"
                     /locus_tag="IVZ67_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8165521.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14722..14895)
                     /locus_tag="IVZ67_001441"
     CDS             complement(14722..14895)
                     /locus_tag="IVZ67_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8165522.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15343..17705
                     /locus_tag="IVZ67_001442"
                     /pseudo
     CDS             15343..17705
                     /locus_tag="IVZ67_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18073..18996
                     /gene="truB"
                     /locus_tag="IVZ67_001443"
     CDS             18073..18996
                     /gene="truB"
                     /locus_tag="IVZ67_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8165523.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19000..19941
                     /gene="ribF"
                     /locus_tag="IVZ67_001444"
     CDS             19000..19941
                     /gene="ribF"
                     /locus_tag="IVZ67_001444"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8165524.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6861 a         3520 c         4392 g         5240 t
ORIGIN      
        1 ataatactta agatagtggg aatcttttaa gtataggagg aaaaagaatg ttacgttttt
       61 ttccgcaaaa aaacaaatat attcaagaaa aaagcgaatt tcaatttaat accgatggac
      121 caattcctca gcacattgcc atcatcatgg acggcaatgg tcgttgggcg caaaaccgtc
      181 gtttgccacg agttgctggc cataaagaag gcatggaaac agtaaagaaa gtcacaaaaa
      241 aagcatcaag gcttggagtg aaagtgttga cgctttatgc cttttcgact gaaaactgga
      301 agcgaccaaa agatgaagta agttttctca tgcaactacc tgtcgacttc tttgatacgt
      361 tcgtaccaga attgattaaa gaaaatgtca aagttcatgt catgggttat gaaaacgtat
      421 tgccagaaca tacgcaagat gctgtaagac gtgcaattga gcaaacaaaa aacaatactg
      481 gaatggtatt gaattttgct ttgaattatg gaagcagagc agagatcgtg actgctgtcc
      541 aagaaattgc cgaagaagta gcaaaaggag aaatacacgc agaagaaatc gatgatgaat
      601 tgattgctaa gcatttaatg acaggtttct tgcctaaaga gttacaagat cctgagctga
      661 tgatccgtac aagtggagaa gagcgaatta gtaattttct gttatggcag atcgcatata
      721 gtgagctgta ttttacaaaa gcgctttggc ctgattttga tggtgcgcat cttgaagaag
      781 caatcgcctc ttatcaaaat cgagaccgtc gattcggcgg tgtaaaaaaa acagaagatg
      841 agaaagggga tcagtcatga aacaacgtgt ggtcacagca gccgtggcgc tagtcttgtt
      901 tattccgatt atatggatcg gaggcatagc ggttgagctt acagcagcaa tcttagcagt
      961 aataggagtt tacgagctgt ttcggatgaa aggattgaca ctcttaagtt ttgaaggagt
     1021 tttatccgcc gttggtgcag tgttacttgt tttgccaaaa gaaagatggt ttttctttct
     1081 tccagagaat gtaagtacat ttatgttatt ttatcttata gttatgattc ttttaggaac
     1141 atcagttatt tctaaaaata cgtatacgat tgatgaagca ggttttcctg tgcttgtcag
     1201 cttgtatgtg ggaataggtt tccagaattt tgtcaatgca cgaacagaaa gcttggctgt
     1261 tttgatattt ggtctattta ttgtttgggc aactgatatc ggagcatata tggttggtcg
     1321 acgttatggt cagcataagt taagtcctga tatttcgccc aacaaaacga ttgaaggtgc
     1381 gctcggtggt attggtagtg ctgttgtagt cgcatttctt tatttattgc tatacccagc
     1441 aaaagaacta tttaatcatg gtatgtttgt catgcttctg atgacgatta ttttttcagc
     1501 agtcggacaa tttggtgatt tggtcgaatc tgcaatcaaa cgtcattatg atgtgaaaga
     1561 ctcgggaaat atcttaccag gccatggtgg aatcttagac cgtttcgaca gtttgctgtt
     1621 tgtatttcct atcatgcatt tatttggcat tatttagtaa gtatacctgc gtttctttgt
     1681 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
     1741 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
     1801 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
     1861 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg
     1921 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
     1981 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
     2041 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
     2101 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
     2161 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
     2221 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
     2281 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
     2341 actttgctgg acccatgaat aactttattt tggcaatcct gttattcatc attctggcat
     2401 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
     2461 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
     2521 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag
     2581 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
     2641 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     2701 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     2761 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     2821 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     2881 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     2941 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     3001 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     3061 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     3121 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     3181 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     3241 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     3301 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     3361 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     3421 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     3481 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     3541 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     3601 cttcgcggaa gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt
     3661 ttggacgaga catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc
     3721 ttagaatttc gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa
     3781 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     3841 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     3901 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     3961 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     4021 gtccttgttt tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     4081 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     4141 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     4201 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     4261 gtgaatccag aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa
     4321 ggagatcctt caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga
     4381 catatcttca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     4441 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     4501 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg
     4561 ccatttgatt tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg
     4621 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     4681 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     4741 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     4801 gttgaagtac gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa
     4861 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     4921 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     4981 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     5041 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     5101 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     5161 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca
     5221 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     5281 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     5341 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     5401 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     5461 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     5521 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     5581 agcggaacga gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     5641 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     5701 atcgccagct cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga
     5761 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     5821 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     5881 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     5941 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     6001 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     6061 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     6121 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg
     6181 acaccgagct atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag
     6241 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     6301 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt
     6361 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     6421 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     6481 tcctttgtca cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc
     6541 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     6601 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     6661 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     6721 attcaaacgt ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca
     6781 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     6841 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     6901 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     6961 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     7021 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     7081 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     7141 agcaaaattc tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     7201 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     7261 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     7321 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     7381 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     7441 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     7501 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     7561 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     7621 tgagatcatt caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc
     7681 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     7741 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     7801 taatccgtta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     7861 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     7921 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     7981 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     8041 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     8101 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     8161 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     8221 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     8281 atatccagca gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat
     8341 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     8401 gcttcaagat ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat
     8461 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     8521 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac
     8581 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     8641 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     8701 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     8761 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     8821 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     8881 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     8941 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     9001 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     9061 aatgaaagaa atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac
     9121 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     9181 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     9241 agctgtccca agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg
     9301 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     9361 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     9421 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     9481 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     9541 ggaattacgc acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt
     9601 cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt
     9661 tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga
     9721 tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc
     9781 agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt
     9841 gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca
     9901 accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct
     9961 tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat
    10021 tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta
    10081 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
    10141 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
    10201 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
    10261 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
    10321 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
    10381 ggcggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
    10441 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
    10501 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
    10561 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
    10621 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
    10681 tattgcgccg cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg
    10741 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
    10801 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
    10861 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
    10921 ttgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
    10981 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
    11041 aaaacgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
    11101 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
    11161 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
    11221 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
    11281 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
    11341 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
    11401 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
    11461 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
    11521 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
    11581 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
    11641 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    11701 aagcgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    11761 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    11821 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    11881 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    11941 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    12001 tagcaatgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    12061 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    12121 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    12181 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    12241 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    12301 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    12361 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    12421 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    12481 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    12541 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    12601 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    12661 agaagaagtc gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca
    12721 cgtagaccat gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg
    12781 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    12841 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    12901 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    12961 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    13021 aattgataaa ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt
    13081 aattcctgaa gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca
    13141 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    13201 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    13261 tcctgtgtca accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt
    13321 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    13381 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    13441 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa
    13501 acgtgcatta ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga
    13561 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    13621 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    13681 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    13741 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    13801 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    13861 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    13921 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    13981 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    14041 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    14101 tgaatgtggt gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    14161 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    14221 gctaactacc gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    14281 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    14341 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    14401 aaaacagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    14461 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    14521 ggcaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca
    14581 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    14641 agttccatga ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    14701 aaaaattcga ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa
    14761 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
    14821 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
    14881 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
    14941 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
    15001 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
    15061 cgatccagcc ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat
    15121 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
    15181 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
    15241 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
    15301 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
    15361 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
    15421 ctttatctta aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa
    15481 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
    15541 cgagtcatca ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga
    15601 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
    15661 acggaagatg agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt
    15721 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
    15781 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
    15841 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
    15901 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
    15961 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
    16021 cttggctggg aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg
    16081 gctgaaaaaa tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg
    16141 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
    16201 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
    16261 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
    16321 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
    16381 agagaaatcg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
    16441 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
    16501 ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
    16561 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
    16621 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
    16681 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
    16741 gggttcttag aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat
    16801 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg
    16861 acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc
    16921 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    16981 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    17041 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    17101 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    17161 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    17221 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    17281 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    17341 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    17401 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    17461 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    17521 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    17581 gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata
    17641 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    17701 aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa
    17761 aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt
    17821 tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc
    17881 aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta
    17941 cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg
    18001 tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa
    18061 tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat
    18121 gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg
    18181 cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc
    18241 gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca
    18301 acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct
    18361 gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca
    18421 atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa
    18481 acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg
    18541 acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga
    18601 acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg
    18661 tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa
    18721 gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa
    18781 ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac
    18841 ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct
    18901 ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa
    18961 ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga
    19021 catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc
    19081 gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa
    19141 gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag
    19201 atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc
    19261 gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca
    19321 ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt
    19381 tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc
    19441 aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc
    19501 tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta
    19561 ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta
    19621 ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt
    19681 tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat
    19741 aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat
    19801 caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg
    19861 aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct
    19921 gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc
    19981 aattacaata aaatgggtga taaataagcc cag
//
LOCUS       DACCZY010000049        19920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCZY010000049 DACCZY010000000
VERSION     DACCZY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.049"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..213
                     /locus_tag="IVZ67_001445"
     CDS             <1..213
                     /locus_tag="IVZ67_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165525.1"
                     /translation="GEVAASLARKDEHGLRDGIGDVVVTLIILAMQNDMDLYECLNQA
                     YNEIKGRTGKMVDGVFVKSSDLEEAE"
     gene            210..413
                     /locus_tag="IVZ67_001446"
     CDS             210..413
                     /locus_tag="IVZ67_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8165526.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            410..706
                     /locus_tag="IVZ67_001447"
     CDS             410..706
                     /locus_tag="IVZ67_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165527.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            783..1199
                     /locus_tag="IVZ67_001448"
     CDS             783..1199
                     /locus_tag="IVZ67_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8165528.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            2105..2449
                     /locus_tag="IVZ67_001449"
     CDS             2105..2449
                     /locus_tag="IVZ67_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8165529.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2454..2735
                     /locus_tag="IVZ67_001450"
     CDS             2454..2735
                     /locus_tag="IVZ67_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165530.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2839..3153
                     /locus_tag="IVZ67_001451"
     CDS             2839..3153
                     /locus_tag="IVZ67_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8165531.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3131..4825
                     /locus_tag="IVZ67_001452"
     CDS             3131..4825
                     /locus_tag="IVZ67_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8165532.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4845..6023
                     /locus_tag="IVZ67_001453"
     CDS             4845..6023
                     /locus_tag="IVZ67_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8165533.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5986..6672
                     /locus_tag="IVZ67_001454"
     CDS             5986..6672
                     /locus_tag="IVZ67_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8165534.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6672..7832
                     /locus_tag="IVZ67_001455"
     CDS             6672..7832
                     /locus_tag="IVZ67_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8165535.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKEAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7842..8717
                     /locus_tag="IVZ67_001456"
     CDS             7842..8717
                     /locus_tag="IVZ67_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165536.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8714..9025
                     /locus_tag="IVZ67_001457"
     CDS             8714..9025
                     /locus_tag="IVZ67_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165537.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9015..9368
                     /locus_tag="IVZ67_001458"
     CDS             9015..9368
                     /locus_tag="IVZ67_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8165538.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9358..9759
                     /locus_tag="IVZ67_001459"
     CDS             9358..9759
                     /locus_tag="IVZ67_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165539.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9752..10156
                     /locus_tag="IVZ67_001460"
     CDS             9752..10156
                     /locus_tag="IVZ67_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165540.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10168..10773
                     /locus_tag="IVZ67_001461"
     CDS             10168..10773
                     /locus_tag="IVZ67_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8165541.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10793..11155
                     /locus_tag="IVZ67_001462"
     CDS             10793..11155
                     /locus_tag="IVZ67_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165542.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11158..11340
                     /locus_tag="IVZ67_001463"
     CDS             11158..11340
                     /locus_tag="IVZ67_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165543.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11357..14776
                     /locus_tag="IVZ67_001464"
     CDS             11357..14776
                     /locus_tag="IVZ67_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8165544.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWIQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            14826..15563
                     /locus_tag="IVZ67_001465"
     CDS             14826..15563
                     /locus_tag="IVZ67_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8165545.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            15573..17864
                     /locus_tag="IVZ67_001466"
     CDS             15573..17864
                     /locus_tag="IVZ67_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165546.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17888..>19920
                     /locus_tag="IVZ67_001467"
     CDS             17888..>19920
                     /locus_tag="IVZ67_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165547.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGEYDYSGNPNLSAKLNASSFS
                     LGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQYTISCEIMLEDGFTGDPS
                     GIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYTSDVPNEWYPLFRDIRTL
                     KPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSAYSL
                     YQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTF
                     TPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTPNISEYKYRGTGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT         6993 a         2971 c         4291 g         5665 t
ORIGIN      
        1 ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga cggaattgga
       61 gacgtagtag taaccttgat tattttagct atgcaaaatg atatggattt gtacgagtgt
      121 ctgaaccaag catacaacga aatcaaagga cgcacaggga aaatggtaga tggtgtattc
      181 gtgaagtcga gtgatttgga ggaagcggaa tgaacaacag acatcgcaga ataacaaaac
      241 taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
      301 cagaagaaac atataaagtg gcaagtcagt gtgttgctga tgcaagtgat gctattcgta
      361 agtttggaat ttcgatatta agtgatgatc gtaaatggga ggaaaaagaa tgaaactaaa
      421 agacggattt tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa
      481 gaatcctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggtcc gctgtgaagc
      541 agaggagttc atttatccat ttcgtggata tgtagaacac gtctataatc actcagcgat
      601 cattcgcatt gaaaacacga tggaatgtga taaatggaca gctaaaagca aagagaattt
      661 agcagtagtg agattggtgg atatggaact aatcaatgac aaataaaaaa gccggatcgc
      721 tccgactaac ataataaaac agacaagttt attatatcac ataaaaggag cagtttgact
      781 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
      841 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
      901 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagag gatgcattga
      961 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggctctta
     1021 gtctgattag tcgtcagata ctctactata gcttctgtga cgtaaataag cactctaatt
     1081 atgaaatagg tcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcta
     1141 tcgcgctgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttgat
     1201 tttgtagggt ttttgtaggg atagtgtagg gtttttgagt gttttaacgt gatattatga
     1261 tagtgtcgaa agattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc
     1321 tcatcgttta attaagcttc gatagacagc agcggaaata ttaagaataa ggatgtgaat
     1381 tttaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat tgtcattatg
     1441 tcactgtggc ggaaagggta gacgcttaaa aataaggtca atacgtcgag ggatagcctt
     1501 aacgttttat gatttgacca tgcaatgttc gattcattgc cagtgactta aggaacctac
     1561 ggaaacaatt catcttatcg gatgccgatg aattggctga ctagtcggga tgctactagc
     1621 agttagaagg cataaaatac tagcgcagac gtgcgccact ctcaggtgta ggttaggaga
     1681 gaaacattag ttggggttat taggaatacg ataacctgct tgcgacaaag ctttgtactg
     1741 tcgcgttggt catgaacaga gacggtattc tgtttcagta ttcgttagca accgagggat
     1801 gtggcagtgg tgaggtgcag gaagtatcag acttgtttgt gtgcaagtta ctattacata
     1861 ttagatcact ctttgagtgg tctttttatt ttgcgtaaag gaggcacgac gatgtataga
     1921 ccacaatact tagaacagaa gcatgaagta atcactgtgc aaaacggtaa cggtgagata
     1981 gtacgaaagt atagaagacc aataaagagc gatacatata aacggaagga aagcaatgaa
     2041 gttattccat tgtatggcaa aagaatagct aagtattaaa taagattgcg aaaggagatg
     2101 ggacatgacc gaggaattct atagatggtt attacagttg ataagagaag atcgtttggt
     2161 taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga aacgagatca
     2221 ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga acgttcatca
     2281 tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc ttatttgttt
     2341 atgtgttgaa catcataatg aagttcatgg cagatatctt acagcgttag ataaacaaga
     2401 gaagaagata gaaagcttcg ctaacttcga tgcaagtgaa aggtggtaag tgcatgatca
     2461 tcaatgacaa tggcagagag tatgatacag aaaagattga agagtattca tcttatactc
     2521 agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat ctgttatcag
     2581 ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat aaagataata
     2641 acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat tattacattg
     2701 acttcgctga agctttcatt ccgatggtga gataaccccc cccttaaaat aaatcgcaaa
     2761 ttttttgggg gtgatgaaac ggagggggct gtcaggaaaa gagatttttt cgaactttat
     2821 catgaaagga gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa
     2881 ttacgccgct ctttaataag ccaattggaa agtcagaaaa caaatattga gccattctta
     2941 gataatgttg atcgttatat cagtttatgg gaaacggcga tatcactgga agaagatata
     3001 tccgagaacg gcattagact ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt
     3061 tctgtcaaca aacaaatggg attgatgttg gataaacttg ccattactcc tgaattggta
     3121 ggtgaagcaa atgaatcaat tcctgagtta taagcatatt gaaaattggt tcaaagctat
     3181 agaagaaggc actatcaagg tatgcaaaga gcaattattg ctaaaaaatt atctagaaga
     3241 aagagtcttt actagagaag atatttactt cgataagcag atggtagagg attcaatcaa
     3301 tataccagca caatactttc cattcgaatt aattccgtgg gaaaaatttc tacaatgttt
     3361 tatttatggt gttcgatgga aaaaagataa aacactagtg ttcaatagat atctttcatt
     3421 aatgggacgt ggtaatggta aaactggttt tgcttcttgg aacaacttct ttctactaac
     3481 cgctaaacac ggtattaaaa attatgatat tgatatctat gccaataatg aaagccaagc
     3541 aaagactagt tttgatgatg tatttaaagt aattaaagat catcctgatt tagataaaaa
     3601 agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca aacttcgtta
     3661 taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc gctttgatga
     3721 aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg gtggtaaaat
     3781 tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg gtccgcttga
     3841 tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg acaaggatgg
     3901 agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata atgaagttga
     3961 tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcag atttaaaacg
     4021 gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac ttacgttcat
     4081 gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt catatgatga
     4141 tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga taggaacagt
     4201 cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat acgataaaga
     4261 tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac aaaacatcaa
     4321 acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc atggaaaaaa
     4381 tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat attatattaa
     4441 aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag aagaagcagg
     4501 ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa aagatctggt
     4561 tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta tgcgttggta
     4621 ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg aaaaaataga
     4681 acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga attttttaga
     4741 tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa atacaggaac
     4801 tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg atttttcaaa
     4861 aggcggtagg atacttcaca aaaaaagcaa cggttccttt agaagaatac ttttgtaaat
     4921 tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa
     4981 atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc
     5041 tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa tttagaaaaa
     5101 aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg tctaataatt
     5161 ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg aaagatgatg
     5221 tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta gaaagtgatg
     5281 ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg tattatcaag
     5341 cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac gctcgtagat
     5401 acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat caaatcaata
     5461 aaatgatgac atcacaattt aaggctttta tggaagctga taatgcaggt gcggtatttc
     5521 aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc aattcaagag
     5581 atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt cacgttccga
     5641 aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct tttttaatgt
     5701 tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt ctctatgaag
     5761 tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc aagattacta
     5821 gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta tttacaatca
     5881 acgaaggaag agagcgcgtt ggaaatgatc gctctgataa ggcgatggca gatgaaatat
     5941 ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac gacaatgaaa
     6001 acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca gggatttatt
     6061 ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat tttggatagt
     6121 ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga cgtatttcaa
     6181 gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt tgtgattaac
     6241 ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat aaaaatctac
     6301 aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac
     6361 ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc ttcatataaa
     6421 acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga atcgtttatg
     6481 gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc
     6541 tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa
     6601 aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt tggacaagga
     6661 gatgtaaaat aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc gaccaattga
     6721 tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta agcttatcta
     6781 aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat caattagtat
     6841 taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat aacgaagtag
     6901 tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta tttgaacgtg
     6961 tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt gttaacacaa
     7021 ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt
     7081 gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg aagcagttca
     7141 agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca gtatggttag
     7201 atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa caagcaattg
     7261 ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc aaacaaacta
     7321 gcggagaata tgctgaaaaa gaagcagaac ctattacagc tttagatgct gtaactatgg
     7381 gcggtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat ccgatttatc
     7441 gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac tggtctaaag
     7501 tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta ttgccagtac
     7561 cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt ggggtagcct
     7621 cagattactt catgggtgta ggatctacac taaaaattga agcttcagat gaataccatt
     7681 ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa cctaaacgta
     7741 acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca actacaaaac
     7801 caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat attctttgtc
     7861 agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat ctgaagaaac
     7921 taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt cctatttttt
     7981 ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac gacaaagagt
     8041 atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat cattcgcgtg
     8101 aggacgaccg atatttctat atcgattcgg atgtcatttt caataaaaga attaatttgc
     8161 gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt tatctaagtc
     8221 ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg gcaaacattg
     8281 taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc gcacagtggg
     8341 ttattaaccg ccctaaagcg aattattgga aaaaggttta tctggattct aatcggctat
     8401 atcgctacct tagagggcaa aaaacaaata taaaaatctg gacagccgag atgtgggcac
     8461 agctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa ttagactttt
     8521 gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac aatgctggag
     8581 taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg
     8641 atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta
     8701 aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga
     8761 taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa
     8821 tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga
     8881 atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga
     8941 acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat
     9001 tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt
     9061 taaagatttt gacgcaaact accaaaagaa atgaactagg aaaaaagatt ggtgtagaag
     9121 atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt gatataacag
     9181 ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg
     9241 ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta
     9301 tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg
     9361 gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact
     9421 tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta
     9481 cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca
     9541 aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta
     9601 acaggagatt cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga
     9661 atggatccca atcatttaaa actagagaac caagatcgct atatcgatca attggtattt
     9721 acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat
     9781 aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact
     9841 gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa
     9901 aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc
     9961 acttaagact caatacatta atttgggttt catcattaga cctacaagaa aatttgagta
    10021 tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat
    10081 gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga
    10141 taaattaaat aaataggagg aacaacaatg gctaaaacaa caacagtaac aacttttgac
    10201 aatataagta ttaaaagaat ttcttttaat tttaagaatg caacaaatgc aatttcaact
    10261 gattgtaatg gacaattaga tggcgaaaca gaaatgcaga gaattgtaaa aaaatgcggt
    10321 tcaacagaag taaaatcgaa atctaaacca atcaatatga cggtaacaat tactgcacat
    10381 gtaccgatgg aagtttatcg acgcttcaat gggttgaaac aagatgaacg tattaaatcg
    10441 ggaatttact cttatggtcc tgattccgta ggggaagatt tctctcttgc tgcagagatc
    10501 gtggatgatt tcgaagaaaa aaataagctg attggtatgt tagcatgcac ttcgaataca
    10561 ggattaacat tctctattga aaatggtgcg gatgaagtag ctgcgttaga actagaaaca
    10621 aaagttatgc aagatgaatt tggtaaattt tatcatgaag caattgttgc agaacttgaa
    10681 gaagacttaa cagatcaatg gatgacgaat ctatctgctg atgtgattaa aaaaacttca
    10741 gttgtgacaa ctacggccac tcaatcacag taaaaaaaac ggaggtagcg aaatgaacga
    10801 agattactca aaaattgaac taaacgatgg aacaattttg aatttagaac ctaaactgaa
    10861 tatcaagaaa ttattgatga tcaatagaga ttttaacaca gacgagtttg caaaaatgac
    10921 tgtgggaaaa ggatccatgg atatttctgt tattcaaggt gcaaaggctg tgtatattgc
    10981 ttaccgccaa gcgaacatga ctgattatat ttcattcgat gaatttatcg ataaatggga
    11041 ttttgatatg gctactgcca gctatattta tcaattgatg atgttcaaac aagcacgcga
    11101 tgcttatcaa aaagaatttg aaaaagcaaa taaggaaaaa aagcttcaaa agtaaaaatg
    11161 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
    11221 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
    11281 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
    11341 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt tcatagctaa
    11401 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    11461 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    11521 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    11581 tagccaacgt ttagccaatg cgaaaaaata ttatggagaa aattccaccg aagttcagaa
    11641 acttgaaaga gaactgatta atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga
    11701 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
    11761 gcaacagttg gatagcgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
    11821 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaagccgaga aattagcgaa
    11881 agcccaagaa tatgtttctc aacaatctga aaatgcggag aaaacgatag atatcctaag
    11941 acgacagtta gaagctacac agtctgaatt tggcgctaca tccacagaag caatgcagat
    12001 ggaagcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
    12061 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atttaatgga
    12121 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
    12181 ggactctgct aacagtgtag gtagttccca gagtaagata caagctaatt ttggtttgtc
    12241 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
    12301 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggagattt
    12361 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
    12421 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
    12481 tgctcaagat gcaatggatt taatggtttc gggtactcaa aacggtttag ataaaacgca
    12541 cgaattaggc gacaatatgg cagaatatag ccaattatgg agtcaaatgg gatattcagc
    12601 tgatgaaacg ttcggaatgc ttcaaaatgg tttagatgcg ggtgcttata accttgataa
    12661 agtcaatgac ttagttaagg aaatgggaat atcgttaaca gatggtcgat ttgagcaaaa
    12721 catggatatg tttagtgaaa gtactagaaa agcttttgaa gagtggaaaa atggcggagg
    12781 aacacaaaaa gacgttatta attccatgat tcaagatttt agcaatatgg atggtcaata
    12841 cgaccaatta aataaagctt ctacaatttg gtctgcactt ggcgaagata acgcgatgaa
    12901 agttgtccaa tctttgactg atgttaatca tacatttgat gatgttagtg gatctgcaca
    12961 aaaaatgaat gaagattcta ctaccccgtt gcaagagttg aacggtaaaa tagctgaatt
    13021 aaaggattca ttagctccta taggcaacac aatcatagat gcactcgaac cagtaattga
    13081 ttttctagga aagatggctg atgcgtttaa taatcttcca caaccagtac aggattatgc
    13141 cgtagcgatt ggcggattga ctgctgcatt tactttatta atgccaataa tagttggctt
    13201 catggctcta ggtggtccta ctacattaat aataggagca gttattactg ctattgctgg
    13261 agttatagca attataaaaa actggggcgc aattactgac tggtttaagg gaatatggag
    13321 taaattcact gattggttgg gtggtacttg ggaaagtata aaagaaggtg cctcatcagt
    13381 ttgggatgga gtcaaagaaa cctggtctgg atttgtagat tggattcaag atatttggca
    13441 aggcgtttct gattggtttg gagagctgtg gagcggatta gttgaaggag cttccaacat
    13501 ctggcaagga gtccaagaga cttggcaaac attcgttgat tgggtttcaa atatttggaa
    13561 cggagtcaaa gaagtatggt cgattatttg ggcagatatt gtagggattg ttcaaatacc
    13621 atggacctta ataacgtcat tgattcaagc tggtattaat attatagtgg gtatttttga
    13681 tgtagctgga cagttattag gcgcagcttg gcaagctgtt tggacaccta tttctgattt
    13741 ccttaaaaac acttgggata ctatgacaca atgggtaagc atcgcttgga atggaattgt
    13801 aactacattc catactatat ttgatccagt tgtggcatgg tggaacggta tatggacatc
    13861 cattagtact acggcttcaa atatttggaa ttcaattagt gcaacagctt ctagtatttg
    13921 gaacagtatc aagaatacaa tcactagttt ggtacaagca gctgctacag taattcaaaa
    13981 tatttggtca actgtatcta gttggttagg tggaatttgg aattcaatca gctctacagc
    14041 atcaaatatc tggaatagtg tgactagtag tataagcaat gctataaacg cagctaaaag
    14101 tgccattcaa agtgtttgga atagtatatc ttcgtggatc agcggaattt ggaacggtat
    14161 caaaaacact gctttgaatc tttggaatgg aattacaagc actattagct ctaaagtaaa
    14221 cgatggaaaa aatgcaattt caagcggttg gtccaatcta acaggtattg tttccgacat
    14281 attcaataat gttaaaagta caattgctaa catttgggaa ggtatcaaaa agactgttag
    14341 cgctccgatt gattggatta gagataaaat cagtggtatt tttgataatt tgaatatttc
    14401 tataccacat attccgttac cacattttaa attgagtggg gaattcaatc cattgaaggg
    14461 aaaaatccca acgttgggtg ttgattggta tgcgaaaggt agtgtgttta attctccgaa
    14521 tattatcggt gtaggtgaag caggacctga agcagtttta ccattgaaaa gatctgtgct
    14581 gcaagaaatt ggtgatcgta tcttgagtag cacatcagtt tcatctaggg cacaaacgat
    14641 tcaacctgtg aacaattacg aattcaattt cacaattgat ggtaacgcag atgaggttac
    14701 tatgaagcaa acaactcaac aaatcattga cagcattaca aaagttcaaa atgataatgc
    14761 ttcggcatgg cgttaaacag gagagtattt ctcctgtttt ttagtattaa aaaggatgtg
    14821 aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat ttgcataaat
    14881 tggctttagc tgaaagacca accataccat ctccaaaaag acagagagtt gaaacttctg
    14941 ttttaggacg gttaggtggc ttagtacaag attattcgtt tgaagatatg tcgtttactt
    15001 tgcactataa ctatttagag gatgcggaag accatcaagc gttcaagcaa tcgttttata
    15061 tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgatgat cccaacgtct
    15121 attatgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt gaatggggag
    15181 agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat gtgcctataa
    15241 ccgtagataa accacagtca tttagtttgc tgaataatag tttagaagaa agttttccaa
    15301 agattatcat cactccttca gctacttcat gccagttcat cttaaatgat tatgtgttta
    15361 gttttgaagg cttagtagta ggaactgatg tagtcattga tagtgattta atgctttgct
    15421 acgaagagca atcggacgga gatattttag atcgatccaa caaaatgaag accatgcaat
    15481 atccgacatt gcaagtggat attaattatt ttaattgtac tggtttgagc aaaatacaaa
    15541 tttatcgtaa tgggttaagg taggtgaaat agatgatcga taatttaata actatttacg
    15601 ataaaaatga cgcgaataat ttagctgaac atttatatga tacacaaggt ttaggcgctt
    15661 tgtcagactg gttaacagcc actgttagca ataaattaaa cggagccgag atatttcagg
    15721 gtacttatcc aataagcgga actaatgcag acttgattgt agacggacgt attattcagt
    15781 gctatgtgga tgaaaatcga gcaaagcaac gtttacggat ttattatgca aagacttctg
    15841 taataggaaa tatgatagaa gtaaaagctg aacctatttt caatgatata agaaaatcgg
    15901 tgttgaataa atatgacagc ggaacagaaa agatcactgc tactcaggca tggcaaaacg
    15961 caaaagtttt agcaaaacca gctattcctt cgcagttttc tttctcatcg ttagtagata
    16021 cgcttgctaa tgtgaagata gaaaaggcga attttttaga attctttggc ggaaaggaag
    16081 gatctatcct agatcgattt catggggaat ttcttaaaga taataacaca ttacgtcatg
    16141 aaacaaggct aggtacggat cataaaatca aagcgattta tactaaaaac ttaactggtc
    16201 tcgacttaga gatagatgct caaagtgttt tagttggagt ttatccattc attagcagtt
    16261 cgtcagaagg agaggatgag atcactctac cagaagaagt cattttcacg gattacgtgg
    16321 aagattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg actgatgtgg
    16381 ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa cagaaaccac
    16441 aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat gaaaaatttg
    16501 ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca cagttaaaag
    16561 tgaatatgtc agcaagaatt gtggaatata cgtttaatgt tttaaccaat tcatacgata
    16621 aattagttgt aggaaacgtc aaaacaaact tcttagaaaa tacagagaat aatgtcaaca
    16681 atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt gatttaatca
    16741 atgatattgt agatcatcaa actgacatga ttactggtca agatggtggg tatgttttac
    16801 tagatcctaa agaagcacct agtcgtattt tgattatgga cacgccagat aagaataccg
    16861 caaggaatgt tttacaaatc aacaatgctg gtattggttt ctctaaaact ggtattaatg
    16921 gaacgtatga caccgcatgg acgttagatg gcggatttaa tgcctcgttt attacagctg
    16981 gtgagatagt agggattact attagaggta ctacattaat tagtgatggt actgattata
    17041 gaacaagtat tgccaatggc aaaatgactt ggtactcaaa aaaagttaac aaagatatta
    17101 tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca tacaccatga
    17161 aaactggtgg tggtttcatg attagaaatc cacaggggaa tttggttttt agtacgtggg
    17221 ataatggcaa taacaaaccg tttctatctt ttggtgcgcc caatttcagg tatagcaatg
    17281 ctagttatat aactgatggc gacggtagtt ctttaagcat taatggtagt gcgggtagtt
    17341 catgggagtt taaagtagct ggcaggacta tgaaatttac tagtgatggt atgctaacgt
    17401 tgccaggttg tttttttggt tcatgggaag atgggaaaat tgcaaggttt gaacaatcaa
    17461 cggtacaagt atataaaaat tttactgtta gaggtactaa aaactcaacc gtaccgacag
    17521 aacattatgg acaacgacta ttgaacgctt atgaaactcc agaatattat ttcgctgatt
    17581 atggagaagc cgttacaggt gatgatggta aagttcgtgt tgatattgat cccatgtttg
    17641 ctgagacagt aaatctaagt cggtatatga cacatgtgac acctacagaa ctagttttgt
    17701 gtgctgttac tcatgaagat gttgaccatt tcatcattga aactagtaag ccaaacgtat
    17761 tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga ttaaaagagg
    17821 atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg
    17881 tatataaatg gctagcagtt tatataattt ggctttagat ttcagcaaag aattaaacta
    17941 caccaaagct attatggctc gtcaaggtga taaagggatt acggtgacgg ttaaaccgtt
    18001 tctaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag gaacaacacc
    18061 atctaaccgt tacgtagata atgttgcaac tagtgtaact agtgaagaag tcacgttttc
    18121 tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg tagaatatag
    18181 aaaagacaat caaattttaa caacgcaaga tatcattttt ttctcactag gagtgtctga
    18241 catttcgcaa ggccaagccg atgaatatgt ttcgcaatta gaagagttga ttcgaaagta
    18301 caacgaaact tttgatgctt ttctggctga aattaaaggt agaatggata gcttaaataa
    18361 acagattact gatttaactg gtcaagctaa aacactacaa gacaagttag atgctctgaa
    18421 agaagaaatt tctaaattag gtaacttaca tgtgatgtac tccaacagca tcgatttcgg
    18481 ggaatatgat tattcgggga atccgaacct tagcgctaaa cttaatgctt caagtttttc
    18541 tttaggtact ggcgcaacag tcgcggatga taatgatgag attgtgttta ccttagatgg
    18601 cacaaatcaa ctatcgaagt acgctagcaa agcgcaagtg ccggtagcag aagggaaaca
    18661 atatacgatt agttgtgaaa ttatgttaga agatgggttc actggcgacc cttcaggaat
    18721 acgtctgcaa cacgcatatt tgccaggcgg ggttgttgtt ttacagacag agacagtacc
    18781 taaaaatgaa ttgaatacgt ggcagacgct tatcggaacg cgaacggtaa aatacacctc
    18841 tgacgtacca aatgaatggt accctttgtt tagagacatc cgaacactta aaccgaccgg
    18901 taaagttaga ctaagaaaaa ttaagattga agaaggctca acagccaccc catatcagcc
    18961 aaacttactt gatgaccctt actggctagg taaagcgcct ttgggtgaga atattgctaa
    19021 taagtctgtt acgtttccaa tcaaatctag cgcctacagc ctataccaag ctaacatgga
    19081 agaagaattt gttttaggtc aaacctatac aattaccatg aaggcaacca aaccacctat
    19141 ccaaacgttc attgtttaca acgaggatag tggagattat agatatggta acttagagcc
    19201 agtagaaggg ttagttgata catggggtct gacttttaca ccgcagaagg ttggagttaa
    19261 ctaccccaaa cggttaacta ttatccagta tccccaatca acaacaggtg catgtcaaat
    19321 cgactggctc aagatcgaaa aaggcaacac acgaactccg aatattagtg agtataaata
    19381 tcgtggtact ggtatgcgtg attcaaacaa tccaaaagat tatgtttggg atctagcacc
    19441 agaatatgtc gaagataact tggccacaga tattaaaatt tctgaaatta ctggtaaagc
    19501 aaacaattat accgatggga aagtatcgga gattaattcg cagttgactg cttcaattaa
    19561 tgaagtagac accacagcta aggatgctca aacaaaagcg aatgctaatg cgactgctat
    19621 agatgaatta gacaataaga tcgatgaacg cattaatgat acagctacta ccacattaac
    19681 agttacaaac gggaataccg gatcagcaaa gctttatcgt gaaggaaaaa cagtttctat
    19741 atattttgtg gctttaaacg gaaaaagcag tggtggaaat gattcaacga tactaacaat
    19801 tccagaaggc tatcggccac caattagttt tgagcaactg gttggctcga tagaccgttc
    19861 tactttgaac agtgctcagt tatctattgg tgcagatgga gccattaaat ggcgaagaaa
//
LOCUS       DACCZY010000050        19812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCZY010000050 DACCZY010000000
VERSION     DACCZY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.050"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            90..1751
                     /locus_tag="IVZ67_001468"
     CDS             90..1751
                     /locus_tag="IVZ67_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8165548.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1821..2183)
                     /locus_tag="IVZ67_001469"
     CDS             complement(1821..2183)
                     /locus_tag="IVZ67_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8165549.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2251..3192
                     /locus_tag="IVZ67_001470"
     CDS             2251..3192
                     /locus_tag="IVZ67_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165550.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3193..4236
                     /locus_tag="IVZ67_001471"
     CDS             3193..4236
                     /locus_tag="IVZ67_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165551.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4242..5312
                     /locus_tag="IVZ67_001472"
     CDS             4242..5312
                     /locus_tag="IVZ67_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165552.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5302..6255
                     /locus_tag="IVZ67_001473"
     CDS             5302..6255
                     /locus_tag="IVZ67_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165553.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6267..7277
                     /locus_tag="IVZ67_001474"
     CDS             6267..7277
                     /locus_tag="IVZ67_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8165554.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7562..8383)
                     /locus_tag="IVZ67_001475"
     CDS             complement(7562..8383)
                     /locus_tag="IVZ67_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8165555.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8480..8758)
                     /locus_tag="IVZ67_001476"
     CDS             complement(8480..8758)
                     /locus_tag="IVZ67_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165556.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9026..11035
                     /gene="metG"
                     /locus_tag="IVZ67_001477"
     CDS             9026..11035
                     /gene="metG"
                     /locus_tag="IVZ67_001477"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8165557.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11514..12101)
                     /locus_tag="IVZ67_001478"
     CDS             complement(11514..12101)
                     /locus_tag="IVZ67_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165558.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12276..13598
                     /locus_tag="IVZ67_001479"
     CDS             12276..13598
                     /locus_tag="IVZ67_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165559.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13753..14529
                     /locus_tag="IVZ67_001480"
     CDS             13753..14529
                     /locus_tag="IVZ67_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8165560.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14522..15091
                     /gene="rnmV"
                     /locus_tag="IVZ67_001481"
     CDS             14522..15091
                     /gene="rnmV"
                     /locus_tag="IVZ67_001481"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8165561.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15084..15968
                     /gene="rsmA"
                     /locus_tag="IVZ67_001482"
     CDS             15084..15968
                     /gene="rsmA"
                     /locus_tag="IVZ67_001482"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8165562.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16419..17048
                     /locus_tag="IVZ67_001483"
     CDS             16419..17048
                     /locus_tag="IVZ67_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165563.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17172..18281)
                     /gene="ugpC"
                     /locus_tag="IVZ67_001484"
     CDS             complement(17172..18281)
                     /gene="ugpC"
                     /locus_tag="IVZ67_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8165564.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18490..18912
                     /gene="mgsA"
                     /locus_tag="IVZ67_001485"
     CDS             18490..18912
                     /gene="mgsA"
                     /locus_tag="IVZ67_001485"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8165565.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19235..19771
                     /locus_tag="IVZ67_001486"
     CDS             19235..19771
                     /locus_tag="IVZ67_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8165566.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6438 a         3572 c         4053 g         5749 t
ORIGIN      
        1 gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat attaattatt
       61 ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg
      121 cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca agcagtagtt
      181 cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa
      241 tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca ttgaataacg
      301 tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca ggagctagcg
      361 agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga gaagacgcaa
      421 aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg caacgaacag
      481 tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa aatgcagaag
      541 acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt ggtgactatg
      601 aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt gctttcccat
      661 catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa
      721 gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga ccaggaacag
      781 atacagaatg gacttataag aaaaatgatc attactggga caaagataca gtaaaacttt
      841 ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta ttcaaagatg
      901 gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca aacgatccag
      961 cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga
     1021 attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc gatcgtgaag
     1081 ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg atcccatcag
     1141 atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga aaattagttt
     1201 cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg
     1261 attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa gtaatcgaat
     1321 atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca acacctgttc
     1381 cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta ctaggcggat
     1441 gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca ggtaattcat
     1501 acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca gcttcatcaa
     1561 cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc aagatcatct
     1621 ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa
     1681 aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa tggacatacg
     1741 ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc
     1801 aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga
     1861 gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct ggcagacgag
     1921 agcgtggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc
     1981 tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat aatcagaaac
     2041 ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga atggttgatg
     2101 agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg gatagatttt
     2161 ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg
     2221 tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa
     2281 cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg
     2341 caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag
     2401 atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt
     2461 tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca
     2521 catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt
     2581 ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca
     2641 gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct
     2701 gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat
     2761 ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct
     2821 gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca
     2881 aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg
     2941 attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg
     3001 gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca
     3061 aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt
     3121 attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg
     3181 agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg
     3241 gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct
     3301 tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt
     3361 tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct
     3421 catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc
     3481 aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct
     3541 caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat
     3601 gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca
     3661 ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt
     3721 gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc
     3781 atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc
     3841 atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat
     3901 caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa
     3961 catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct
     4021 gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct
     4081 tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta
     4141 tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga
     4201 ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag
     4261 tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg
     4321 tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa
     4381 aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg
     4441 gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa
     4501 tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga
     4561 tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag
     4621 agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac
     4681 gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga
     4741 ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca gcgcttgatg
     4801 tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat
     4861 caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg
     4921 tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc
     4981 aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc
     5041 gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat
     5101 tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg
     5161 aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga
     5221 caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt
     5281 acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag
     5341 tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt
     5401 tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc
     5461 cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat
     5521 ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc
     5581 tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa
     5641 ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat
     5701 ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca
     5761 ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc
     5821 atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg
     5881 ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg
     5941 atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg
     6001 ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct
     6061 gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct
     6121 gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat
     6181 gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt
     6241 ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga
     6301 cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc
     6361 atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca
     6421 cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag
     6481 caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct
     6541 tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc
     6601 gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga
     6661 taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata
     6721 atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag
     6781 ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag
     6841 aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat
     6901 tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt
     6961 atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag
     7021 attatcttga acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat
     7081 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
     7141 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
     7201 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
     7261 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
     7321 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
     7381 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
     7441 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
     7501 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
     7561 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
     7621 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
     7681 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
     7741 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
     7801 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
     7861 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
     7921 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
     7981 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
     8041 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     8101 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     8161 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     8221 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     8281 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt
     8341 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     8401 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     8461 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     8521 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     8581 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     8641 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     8701 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     8761 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     8821 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     8881 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     8941 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     9001 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     9061 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     9121 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     9181 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     9241 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     9301 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     9361 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     9421 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     9481 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     9541 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     9601 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     9661 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     9721 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     9781 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     9841 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     9901 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     9961 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
    10021 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
    10081 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
    10141 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
    10201 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
    10261 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
    10321 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
    10381 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
    10441 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
    10501 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
    10561 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
    10621 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
    10681 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
    10741 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
    10801 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
    10861 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
    10921 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
    10981 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
    11041 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
    11101 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
    11161 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
    11221 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
    11281 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
    11341 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
    11401 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
    11461 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
    11521 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
    11581 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
    11641 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
    11701 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
    11761 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
    11821 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
    11881 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
    11941 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
    12001 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
    12061 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
    12121 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
    12181 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
    12241 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
    12301 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
    12361 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
    12421 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
    12481 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
    12541 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
    12601 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
    12661 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
    12721 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
    12781 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
    12841 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
    12901 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
    12961 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
    13021 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
    13081 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
    13141 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
    13201 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
    13261 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
    13321 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
    13381 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
    13441 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
    13501 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
    13561 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
    13621 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
    13681 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
    13741 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
    13801 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
    13861 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
    13921 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
    13981 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
    14041 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
    14101 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
    14161 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
    14221 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
    14281 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
    14341 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
    14401 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
    14461 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
    14521 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
    14581 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
    14641 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
    14701 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
    14761 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
    14821 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
    14881 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
    14941 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
    15001 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
    15061 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
    15121 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
    15181 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
    15241 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
    15301 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
    15361 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
    15421 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
    15481 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
    15541 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
    15601 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
    15661 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
    15721 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
    15781 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
    15841 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
    15901 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
    15961 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
    16021 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
    16081 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
    16141 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
    16201 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
    16261 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
    16321 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
    16381 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
    16441 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
    16501 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
    16561 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
    16621 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
    16681 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
    16741 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
    16801 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
    16861 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
    16921 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
    16981 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
    17041 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    17101 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    17161 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    17221 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    17281 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    17341 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    17401 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    17461 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    17521 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    17581 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    17641 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    17701 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    17761 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    17821 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    17881 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    17941 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    18001 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    18061 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    18121 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    18181 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    18241 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
    18301 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
    18361 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
    18421 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
    18481 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
    18541 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
    18601 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
    18661 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
    18721 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
    18781 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
    18841 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
    18901 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
    18961 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
    19021 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
    19081 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
    19141 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
    19201 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
    19261 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
    19321 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
    19381 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
    19441 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
    19501 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
    19561 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
    19621 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
    19681 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
    19741 tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga
    19801 ctgcttgttt tt
//
LOCUS       DACCZY010000051        19789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCZY010000051 DACCZY010000000
VERSION     DACCZY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.051"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(811..1764)
                     /locus_tag="IVZ67_001487"
     CDS             complement(811..1764)
                     /locus_tag="IVZ67_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8165567.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1787..2542)
                     /locus_tag="IVZ67_001488"
     CDS             complement(1787..2542)
                     /locus_tag="IVZ67_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165568.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2539..3498)
                     /locus_tag="IVZ67_001489"
     CDS             complement(2539..3498)
                     /locus_tag="IVZ67_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8165569.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3491..4441)
                     /locus_tag="IVZ67_001490"
     CDS             complement(3491..4441)
                     /locus_tag="IVZ67_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8165570.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4591..5043
                     /locus_tag="IVZ67_001491"
     CDS             4591..5043
                     /locus_tag="IVZ67_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8165571.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5132..7399)
                     /gene="gshAB"
                     /locus_tag="IVZ67_001492"
     CDS             complement(5132..7399)
                     /gene="gshAB"
                     /locus_tag="IVZ67_001492"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8165572.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7586..8584)
                     /gene="ftsY"
                     /locus_tag="IVZ67_001493"
     CDS             complement(7586..8584)
                     /gene="ftsY"
                     /locus_tag="IVZ67_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8165573.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8595..9404)
                     /locus_tag="IVZ67_001494"
     CDS             complement(8595..9404)
                     /locus_tag="IVZ67_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8165574.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9413..12994)
                     /gene="smc"
                     /locus_tag="IVZ67_001495"
     CDS             complement(9413..12994)
                     /gene="smc"
                     /locus_tag="IVZ67_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8165575.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13014..13700)
                     /locus_tag="IVZ67_001496"
     CDS             complement(13014..13700)
                     /locus_tag="IVZ67_001496"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8165576.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14048..15829)
                     /locus_tag="IVZ67_001497"
     CDS             complement(14048..15829)
                     /locus_tag="IVZ67_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8165577.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15855..16769)
                     /locus_tag="IVZ67_001498"
     CDS             complement(15855..16769)
                     /locus_tag="IVZ67_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165578.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16796..17758)
                     /locus_tag="IVZ67_001499"
     CDS             complement(16796..17758)
                     /locus_tag="IVZ67_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165579.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17758..18702)
                     /locus_tag="IVZ67_001500"
     CDS             complement(17758..18702)
                     /locus_tag="IVZ67_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165580.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18703..19707)
                     /locus_tag="IVZ67_001501"
     CDS             complement(18703..19707)
                     /locus_tag="IVZ67_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165581.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5477 a         4260 c         3524 g         6528 t
ORIGIN      
        1 cttcaaaaaa aacgaatagt tttggattga tattaagcga gtataaaatc aaagcagaag
       61 agagttattt ctatcaatta ctaaaacggt ggagtaagct tcccgccgat ttttatcgaa
      121 aaatgcagat catcaactat caaccgcttg ctgagacaca tgcctaaata gaggttgtga
      181 aatcagtgct ttgagctaat accgaaggtc agctgattga acattgttta gaagagattg
      241 tgacaactgc gatttgcttt gagtaataag aacgctggac agaaaatagc ttctcatatt
      301 ttctagtcta gtgggcttat tgccgagaag cagcagatgg aacatcgttt agaagagatt
      361 gtgacaactg cgatttgttt tgagtaataa gaacgctgga cagaaaatag cttctcatat
      421 tttctagtct agtggactta ttgccgagaa gcagcagatg gaacatcgtt taaagaagag
      481 atcggtacaa aataaaaata tctaaattta agttttatct aagtttcgct ttgtaccttt
      541 gattcaaatc aaaggaggcg atcttgatgc tgaaaaaatg gatcacacta ttttttgcca
      601 ctccccattc tagaatcaac ccgtatgatg tagataatga attcacgttg aaaaaagatt
      661 tctcaaataa ataaaaacca tccgtaaaat catcaatacg attttacgga tggtttttga
      721 taacttcttt gaggataggc tcttgctatt taagaatgta agaaacatta ttgttacata
      781 gtatgagagt ttaatcctct gttatttatg ctatttaaat gcttgattca catcttcgat
      841 catcagtttc attgattcta aaccgccccc gcttaaatac cagacatctg gttcaaggga
      901 gatgatctgc tgatttttac cagcattcgt ttgtgctacc agttcattgg cagaaatatc
      961 atctttgctg tcgtctccac caatagcttt tgttctgtct actacaaata agatatccgg
     1021 atttttttcc agcacatatt cataagaaac actttgaccg tgagtggatg cttcgatttg
     1081 atcatctgct tgttcaaagc caaataaatc atggatgaaa ccgaatctag aaccagatcc
     1141 gtaggctgat aattgccctt cattcacaag tgtgactagt gcttttttgt cagtagcttc
     1201 tgctttcttt ttcgtctgat cgattgcact agataattcc tctaattgag ttttagcttc
     1261 ttcttgctta ccaaagatct cccctaaagc agtgacattt tgctggaaag attcccatgg
     1321 atttttcgca tctaaagaaa ggaaaatggt cggagcaatt tgacttagat ctttttgaaa
     1381 atctctttgt cggccagaaa tgatgattag atctggctga agctgattaa ttttttctaa
     1441 gtctggttct tttattccac ctgctgattc tactttttta taggatgaaa gatactcagg
     1501 aaggctgctt gtagctgctc caactacctt atctccaaca ccaagagcat ccatcgtatc
     1561 taaagaacca ttgtcgaata cgacgacttt ttctggatta tttggcacct cgatttggtc
     1621 tccattacta tctgttatcg tcaatgtagt ttcagtttgt gtgctgcttt tttttgtgtc
     1681 tgcctgactg gtgttgttgc tatttccaca tgctgctaaa ccaaatgatg ctagaatcaa
     1741 gatacctact actaattttt tcatctcttc tctcctaatg ttagttttag aaataaagac
     1801 agaatctgcg gccttcaatc tcacaaatcc gtatgtccat ttcgtataat tcatccaata
     1861 catctttttt tatcatttct tgggtcgttc cttgcgtaaa taactgtcca tctttcattg
     1921 caacaatttc gtctgcataa cttgcagcaa agttgatatc atgaagaaca ataacaattg
     1981 ttttcccgat ttcttgtact aaatttttta aaatctgcat gatttgaacc gcaaaattca
     2041 tatctagatt gttcaaaggt tcatccagta aaatataatc cgtgtcttgc gctagcacca
     2101 ttgcaatata cgcacgttgg agttgaccgc ctgaaagagt gtcaatcgat tgatccgcta
     2161 tttcttctaa cccaaggtgt tccagtgatt cctgaacgat ttcctcgtct tttgtcgtca
     2221 attttccttt cgtataagga aatcgtccaa attctactaa ttctttcacg gtgatttgga
     2281 catttatttc attcgattgt cgtaggatcg ataatttttt ggctaattca ttggatttcc
     2341 aagcctttac ttcgttatgg tctaaataga tttcacctgt atcttttggt atcaaacgac
     2401 tgatcatcga caacaccgtt gactttccag caccatttgg accgataaaa gcagtcaact
     2461 tcttttcagg aatcgttaac tgtacatctt taaccactga ttttccatgg tacagtttat
     2521 tgaccttttg taaatcaatc ataggatact cttcctttcg cgaatcaatt ttgtgacaaa
     2581 ataaattcct ccgacaaatt caacgacgac actcactgtt gtattccagc cgaaaatctg
     2641 ctctactgcg agctgtcctc ctgtcagaaa cagcatagct accagaaagc ttccgataaa
     2701 tagctgacca tgacgataag tagaaaagac acgataactg atatttgcga caataaaccc
     2761 taaaaaagta actggaccca caagagccgt tgatgtccct gtcaaaagac tgacacaaca
     2821 taaaatcaaa aattcaaaac gatctgtgtc tatcccgagg tttactgctt gttcatttcc
     2881 gagatggaaa acgtctagca catgtcgata tcgaaataac aggtaccagc agccgataat
     2941 gatcaaagaa gcaggcaaaa gatactgact ctggatattg ccaaagcttg caaataatct
     3001 gccttgaagc aaatcatatt cattcggatc catgatgact tgtaaaaagg tactgacact
     3061 accaaaaagc gtacctaaaa tcatccctga catcagtaat aaaaaaagat tcgctctcat
     3121 ttttttcaaa agaaagaaaa ataacaatag actcagcccg accatcagcc caacgttcaa
     3181 taagaaaaaa actgtacctg tctgctgaag catcgtcact cctccgataa agaagaaaag
     3241 gaccgtttgg acaaggacat ataacgactc aagtcccaat actgaaggag tcaaaaactg
     3301 gttatgcgta attgtctgaa agctgattgt ggcactgctg acagctagcg agacaaggat
     3361 aaatgcaaaa attttcttac tcctcaatgc acatgcaaaa tgccagtttc catatgtatg
     3421 gatcgtcaag tacattcctg taacaaataa aacaagaaca agcataccaa taaaaataga
     3481 tggctttttt ttaagcatat cgtcgtcccc ttatcagaag ataaaggaag atagccccac
     3541 cgatgattcc aacgatcaca ctgactggta tttcataagg aaaaatcaac acgcgagcga
     3601 caagatcgca aaatactaag aacaaactac ctgctaaagc agttacgcca attgttttgt
     3661 gcacttgatc tccatatatt ctagagacaa gattcggaat gatcactccg ataaaaggaa
     3721 tgcttcctac catgatcaac atgactgcac tagccaatga tactaagcca atgcctaaaa
     3781 actgcatctt ctggtaatct attcccagat ttactgcaaa cgattcgccc agaccaatga
     3841 tcgtaaatcg gtaagcaaat agccaaacca agagcaacaa tggaacaaca gcatacaata
     3901 actcataatt tcctcgtatc acagtcgcaa aattcccttg aagccatgaa gtcatatttt
     3961 gtaccaactg gaattgataa gcaaaaaaag tagcaactga accaatgata ttccctaaca
     4021 tgactccaat caacggaagc atcaattgat ttttctggag gaacaatcgc gtaagggata
     4081 aaaaaagcaa tgttcctgca aaagcaaagc aaaatgctgt caaggatcgg acaatcaagg
     4141 atgcaccagg taggaaaatc atagccacta aaatacctaa acgcgcacta tccatcgtgc
     4201 ccgcagtcgt tggtgaaaca aatttatttt gggtcaaatg ttgcatgatc aacccacaaa
     4261 tactactggt cgctcccaca aggatcaaac aaatcgttcg tggcaaacga gtcactagca
     4321 agatttgtaa gctttgccca tctaactgga gcagttctga caattgtaca ttcgatactc
     4381 cgatgaacaa tgaaatacta cttaaaataa ctaataatgc tactagtcgt ccgtagcgca
     4441 tccaatccac ctttctaatt gagaatgatt ttcattctta ttttaagtga atcgttgaac
     4501 aaaagcaacc gctttttcat atgttttagg tattattggg aatatgctat aatcgtaaga
     4561 aagacaaaga gaagaaggag ggtatttttt atggcacaag atgatgtaaa aagccatttg
     4621 gataagggac tttatggtac acctttagtg aaccctgaag aacagcataa atatttaggg
     4681 acattcaggg aacgctgcta tcttagtatg actgttgctg aaatgatcca accaaaaaac
     4741 aaagaaaact ttttaaaaga actcaaaaaa catccagatg cttccatact cttgaatggg
     4801 gctctacccc tttctgtcca aaaccaatat atccagctag caacgaaaaa caatagccgt
     4861 ttcactgtag tcaacgatta cgtcactgca aatcctgagg aattcggatt gctcttaaca
     4921 gcaaaagaag cagtaaatga acctgtgatt gatatcgaaa aaaaataccc agaagaaaac
     4981 tcttcgccag ctgaaaaaga aaaaccaaaa aagaaaaaaa gcttctggga tcgtttattt
     5041 taaaacgaaa aaacacataa atttttacat gcaaaaaaag cttttgatca gattgacatc
     5101 aaataggaca atctgtcaaa agctttttct tttaaaatac ttcaggatat aacaatttca
     5161 atacattcat cgtcaatctt ctgccttttc ctgcaaaagg ataaacatgc atatgcatgg
     5221 ctggattgaa atttgcttcg atgatcccat atgctttttt atcagttgta ggatcgattt
     5281 ctttatctgg tataatcaaa tcgataccac taatcttagc tcctagtgct tctaccgcag
     5341 aaacggcgat ttttttgtaa gcttcagaaa attcatcagt catatcgatc gaatcaccac
     5401 ctgtactgat atttgagttc tcccttagat aaacaatttg attggcttgg ggaactgatt
     5461 cgatcgtcaa cccttgttct ttcaacatca attgttctaa ttcacctaat tggatcaatt
     5521 ctaaaggtgc acgatgattc gttccacgca aagggtcact atttttttct tcaacaagtt
     5581 ctttcactgt tcggatactg tcaccaataa cattagcagg gactcgcaac atgatggctt
     5641 ggacttctcc gtcgatcaca aagaaacgat attccgttcc cggcataaat tcttctacta
     5701 aaaccgaact gtcttcacga aaagcaatgg ctaaacctgc ttgataatct tccagactag
     5761 ctccttcctt gaaaatcgtg atacctaacc cgtaattggt tgatttaggt ttgatcacaa
     5821 atgcctgctc ggcaaatctc ggataagctt ttaccgcttc ttccatggat gaaaattctg
     5881 cgcctcctgg tacccggaac ccagcttctt ttaagacttt tttcgttact gttttgtttt
     5941 ccatgataag tgggacgata tagctgtcct tacttgtcat gtttgcattt ttcacatatt
     6001 ctacatggtc ttggaattgc agacgcaaaa attgatccga ctcatctaat actttgactt
     6061 ggacaccttt ttggatggca tcgaacataa atatctgagt agaaagttcc atttcgcgaa
     6121 aaccggccaa ctgataggga cgttcatggc tttctttata atattgatgt cccaatgcca
     6181 cagcaacttc ttgctgactc gatagctgtg cttcttgata gagtttcgct gctaacgttt
     6241 gttcaggatg atccatttga gtcaatgctt cctctactaa ataaagagaa tccaaccaat
     6301 ccaattcaac cagcatttgc ttcattcctt caagaatttc tctaccttct tgatgaaatt
     6361 ctgtttgatc tgatggttgt tctagtgcta ctttgttatt tcgagtcgta ccttctgcta
     6421 cccattggtc acagtcttct ttttcttctg tccataacat gtacatcagg aataattgta
     6481 agaaccgaac ttgctctgga ttgataccca atcttgcata aggattcaaa tcgatattcc
     6541 gcaattcaat ataacgaaca cctgttgttg ctaaatctgc gactttcttt ccgcctcgga
     6601 aacgaagcgg tgtataaaat tctttctcct ccgaaagttt cccttcttcg atcattcgct
     6661 cgatatctgc taaatactgt ttcatagaag cataggaaac tttcacattt ggatgattcg
     6721 tatagcccag cgcactgttg cgaatactgc gaacaggttc ttgcggatgt gattcatcta
     6781 aaaagtagtt cttctcactc attggtgaag caccgaataa ataagtgatc atccagcgat
     6841 agcgcataaa gtttctcgct gttttcaaat aaagttctgt tttgaattcg gaaaaatctt
     6901 gaaattcttc ctgatggctg aacaatgttc ttagcaactc atcgccaaat tcaaaattga
     6961 aatgaatacc gcttaccatc tgttttcgct tcccgtattt ttttgctaaa taacgtcgat
     7021 acaagacatc ttcgaaattt tttaattttg caataatgat ctcttcgtct ttttccggta
     7081 aggcaggtgg catacttaat ggccagatca tctcttcttt cggtatagaa cgagcagtca
     7141 catcataaac agcagcgaga tactgaaata attcgggaat agaatcggta acaggggtga
     7201 tcatctctgt ttgtgtttcg ctaaaatctg tttggatata ggggtgatag gatcgatcgc
     7261 caaagattgc tggatgatcg gtttttgcta gatttcctgc aagatcaacc cgctgtcctt
     7321 cccgttctat accaaatcga gcttggtcga taaaagggcg cgcattgaca tgcaataata
     7381 attgcttaaa attcatcatt aagtgacctc caattcatat gaatctaatt ataatcaaaa
     7441 agtctaagaa aaagaacgaa attgtttgag aaccgcttac aaataagtat aaaaatgaag
     7501 gcatcaaaaa cacagctatg ttaaaaacaa tcattcaaac aaggagctgc aacttttgtc
     7561 acagctcctg ctttttagac atgggttatt cttcttttag taatcctttg aacaatccaa
     7621 cgacaaaatc atttggatca aacggctcca agtcatcgat tccttcacca agaccgacca
     7681 atttcactgg taaatgcaat tcattacgga tagcaagtac gataccgccc tttgctgtac
     7741 catctaattt tgtcaaaaca agaccggtca catctgttgt ttctttgaat tgttttgctt
     7801 gaaccatcgc gttttgacca gtggttgcat ctaatacaag aagtgtttct tggggtgctt
     7861 ctggtaattc ccgctggatt actcgtttta ttttttctaa ttcgttcatc aagttcactt
     7921 tattttgcag tcggcctgca gtatcaacta gtaaaacatc tgcctgctct gtctttgctc
     7981 tttccaaagc atcgaatacg acagctgctg gatcgcctcc tgcattgcca cgaacaactt
     8041 caacaccagc gcgttcgccc cagacaacta actggtcgat tgctccagcg cggaaggtat
     8101 ctgctgctgc catcaatact tttttacctt cgtttttgaa ctgatgggca agtttaccaa
     8161 tacttgttgt tttccctaca ccattaacac cgacaaacaa aataactgtt agtccgtctt
     8221 cttggatatt tagttcattt acttcattga ttccttccgc ttcatagaga tccacaagtt
     8281 tctcgatgat cgcattttgg acttctgccg attttttcgc atttctgagt ttcacttctt
     8341 gacgtaatgc ttcggtgatt cgtatcgctg tttcaaaacc tacatctgca ccgataagtg
     8401 tttcttctaa ttcttcaaaa aagtcttcat cgactgttcg aaaattggca aacaactcat
     8461 tcatccgttc accaaatgtt ttacgcgtct tttctaaacc cttgtcgtac ttctcctgta
     8521 cttcaacttc ttctgttttc tcttcgccag taaaagcacg cttgatttta tcaaaaaatc
     8581 ccatttgact ccccctactc taatacaaat ttttggatca ctttggctac gccattttct
     8641 tcattgctgg ctgtcacgaa atctgctgct tctttgacga aaggcacagc attctccatc
     8701 gctacgccta gtccagcata ttggatcatc ggtaaatcat tttcctcatc accgatcgcc
     8761 atgatttctt cttgtttcaa accaagatct tttgccaata aagaaatacc ataagctttt
     8821 gtaacacctt ttggcataaa ttccaaaagc atacttcttg ttttgatgat ctcatacatt
     8881 tcatggaatt ctgctggtat ttctttgatt ttaggatcta attcttcttg gtcataagcg
     8941 attactgcct tattaagcac aaattcatct gtaatatcgc ttatatccgc tggttggaat
     9001 tgcagcagct tattcaacac attgtacaag gattgcttcc ttggggcact aggcagctgt
     9061 aaaaccacat tgtcagacaa aacatctagt ggcagatcca attgctgagc cagtttgtat
     9121 aagcgttgga tatctgatac ttccatcact ttcttttcca tgatttcacc agtatcattt
     9181 ttttgtacca aaccgccgtt gaaagtgatg ctgtagtcac cttcatctac cagtcctaat
     9241 tcttgcagat aatgcgccat cgcagctaag gggcgtcctg tacatagaac gatcttgacc
     9301 ccttgctgtt ttgcttgtgc taatgcttgt ttattctcat ctgaaatcag cttttgttca
     9361 tttaataatg taccgtccaa atcgattgct actaatttga tcattttcgt tcctacactt
     9421 tcgtttcgtt gattttcgcc tcttcattga tatcttccat ccgtacagaa acgatttttg
     9481 atacaccgga ttcctgcatc gtcactccgt acagtacatc tgctgcgacc atcgttcctt
     9541 tgcggtgtgt gactacgata aattgtgtgt catcttggaa atcgctcaag taacgaccaa
     9601 aacgtttcac gttggcttca tctaacgctg cttctacctc atctaagata caaaacggaa
     9661 caggacagac acggataatg gaaaagagca acgcgatggc tgtaagtgct cgttctcctc
     9721 cagaaagcaa acttagactt tgcagttttt tgccaggagg ttgtacttca atctcaatac
     9781 ctgttttcag caaatcagtt ggttccgtca gtactaattc agctttaccg ccgccaaaca
     9841 tatttggaaa gacaattttg aattcttgac ggatagcttc aaatacttct ttgaatcttg
     9901 tccgcacttc atcatccatc tcatccatcg tttcaaacaa ttggtttttc gctgctaaca
     9961 gatcatctcg ctgtgttgct aagaatgtat gtctttcact gacttgttcg tattgttcga
    10021 ttgaatttaa gtttactggt cccagttttt caatctgttc ttttaaggat gaaacctttg
    10081 ttctactgtc ttcgatatcg gttgtttctt gatagtcttg ggaagctttc tcaaatgtca
    10141 gctgatattc tgactgcaga taggtcaaat gattatctaa aatcatttca gctcggtctt
    10201 tttggacttc taatttggat tgttccgaca gtcgtgcttt ttgctcacga ttacgctctg
    10261 ccaagactgc ttcaagctta tcgatctcct tttgccttct atctcgctgc tctttggctt
    10321 ttgccaactc ggcttttaat gtttctcgct gagcagataa ttcgttaatt tgtttttcca
    10381 aactttcttc tgtgacttca tgatcagaaa agtctgctgt caatgtcgcc aactgctttt
    10441 ctatcgcttc ttgacgttcg gttgcctctg ctttttggac tcgggcaccg cggagctgga
    10501 tctgcagatg attaaactgc tctttcaaga cagcaagatc tgcttgttct tgtgcttttt
    10561 gactttgtac ttgtgctctt ctggcttcca tctggtcgct ttcttgactt aacgatttga
    10621 tttcttcgtc gattttttgt cgttgcgatt caagatctgt ttgtttgtct ttcaattcgt
    10681 tcgtttgctt ttcgtaatct tcgataaact gctgcagttc acgtgtctca aaagttgaga
    10741 tttgcttttc tttttcaaaa cgttcaagtt catttatgat gttttgtagc tgatttgtcg
    10801 cttcttgttc ttcaaaacgt aattgttcgc ctcgtgtacg caaaacttct tgttcttcgg
    10861 ctaaacgagc tgtttcttgt tgcagctcct gaactttttt ttcttgtgct tggagttgtt
    10921 tatctgcttc ttcaaattca ctagtcaact gtttcaattc ttgattttgt acaaacaaac
    10981 tgccttggtt tcctcgttta ttcgctccac ctgtcatgga tcctccggca ttcatcacat
    11041 caccttctaa ggaaaccact cgatattgat agcggatcgt ttgcgcgatt gcattggcac
    11101 tagttaagtc tttggctaaa aggatcgttc ctagcaggtt ttgaaccaca gtttggattt
    11161 gatcgggata actgacttgt tcactggcaa tccctataaa cccttctaca gctgctgctt
    11221 gtgtcaatat atgagccggt aattgtcttg gtttgattgt cgtcagtgga aggaatgttg
    11281 ctcttcctcc tctttgctgt ttcaaatacg tgatggcttg ccgagcatct ttttcatttt
    11341 caacgatgac atgctgggct gccccaccta aagctgcttc gatagctaac gtgaaatctg
    11401 caggaacatc gatcagttca gccactgctc cgacaatacc cgaaagctgc tgtttatgct
    11461 gcaagaccaa tcgaacccct tggtaaaaac caaaatagtt ttcttggatt tcttgaaggc
    11521 ttttcttacg agcacgtagc tgctggactt gattcattaa ttgatacatt ttaggctgtt
    11581 cttttgctaa tgcttcttgg aactttttcc cgttttgttg gatcatttct aacttctctt
    11641 ttgtttcagt taaagatgat tttagctgcg cctgcttttg tgtcaattct tctttttgta
    11701 agatcaatcg gtctactgat gcttctactt ccgattgctt ggctaacgtc tgcttgcttt
    11761 ttgctgtttc ttgtatatat tgacgttcta gatacttcag ttcgtttcct acagctgctt
    11821 gttcttgcat caaatcaaca tattgatctc ttaattctgc caataattct ttcgttgatt
    11881 tttggtattt ttctaattct tgctgtgttt ttatgagatt tgcttcagct ttttggatct
    11941 ctgtttcttt ttctgctgct gctttcatca agctttcttg aagcttttca aaatgtttaa
    12001 cctttttttg tgcttctgct aatgaagttt gatattcttg cgagcttttt tgtgtatgct
    12061 ttgtccgctc ctgcaaaaca tctttttgtc cttctgtctg cttcagtttt tccgaaagat
    12121 ctaataatac ttgctgattt ttttcgatca gacgatcggc ttgcgcattt tctttacgct
    12181 gcttcgccaa aatggattct tgttcttgga tcgactcaga tagtttgcct aattctagat
    12241 tgaatttcgc aagctgtgct tgtttgttgt cccaatcttt ttttgctgtc ttgatttcag
    12301 caaccatcaa agagacatcg gtttgagtca gtgtttcttt tagccgtaaa aactcctttg
    12361 ctgcctcact ctgggcggcc aaaggagtta actgttcttc caattcgtgg atgatgtctt
    12421 ggacgcggct gaggttgtct tctgtttcaa aaagcttctg ttctgctttc tttttccgct
    12481 gcttgtattt caagacacca gctgcttctt caaagatccc acgacggtct tccggtttac
    12541 tactgaaaat cgcttctact tttccttgag aaatgattga gaacgattct tttcctaatc
    12601 cagaatctaa gaacaattct tggatatctt ttaatctgca gctttgttta ttgatgaaaa
    12661 actcgctttc cccagtccgt cgataacggc gggttacact gatctcattg tactccaatg
    12721 gcagataatg atcactattg tctaaaatca ctgtgacttc tgcaatattc agctgcttac
    12781 gggtatcgga accggcaaag atgatatccg gcatttttcc gcctcgcaga cttttagcag
    12841 attgttctcc taatacccag cggatagcct ctgtaatatt gctttttccg ctgccatttg
    12901 gtccaaccac tgctgtgacg ctattttcaa aatcaatgac tgtcttgtct gcaaatgatt
    12961 taaaaccggc tatttcaatt cgtttcaaat acaaagcttt ccctcactta cttttacagt
    13021 gctgccaatg cattttccgc cgcagcttgt tctgctaatt ttttcgattt cccttgtcca
    13081 gtgccaatca attgatcatc tacataaact tcgatccaaa atactcgttc atgagctgga
    13141 ccttcttcat tgatcaatcg gtattcgatt gatacatcac ctgatttttg caagacttct
    13201 tgtagttttg ttttatgatc catctcatgt gaaaaagcac ctgctttgac tttaggaaaa
    13261 atcacttttt cgatgaatgc atgggtcgtg tcgaatccct ggtcaagata taacgcgcct
    13321 aaaaaagctt cgaataggtc gcaaagcaat gctggtcgcg tacgtcctcc tgaattctct
    13381 tctcccttgc ccaacaaaac atattgatca aagtgacatt ctttggcaaa tttcgacaag
    13441 ctgtcttcgc gaacgatcgc agctcgtgtt tttgtcaatt ttccttctgg caaatgagga
    13501 tataaacgga ataaatactg agaaaccatc agttctaaaa cggcatctcc taaaaattca
    13561 agtcgctcat tatccgataa ttgcagattg cgatgctcat tcacatagga tgaatgggta
    13621 aaagcttgtt caagcaaatt tagatcgtga aaaacgatgt tatagttttc ttttagttct
    13681 tttgtcagct ggttgtccat tttccacatt ccttttatgg atattttttc gatacttcct
    13741 tattatactg gttttttgtc aaatttgaaa tggttcggtc aaaaggtaag taaagcttta
    13801 ataatcctag tgattgacga gaagttgtga aaaattcgtt tagtttcgag tatgaagaaa
    13861 aaattttgtg aaaatggctt tccatatttt tgcataaatt tgacttaata ccgagaaact
    13921 ggactttgaa caccgtttat tggagaagtc atgaaatcag cgccttgacc tgaatattag
    13981 agaaataaac agaaaaatat caaaaaaccg aaaaaggaca tcgccctttt tcggtcattt
    14041 ttagtgatta tgaacgtgaa tctgctgtaa cagaaacatt tgcccatggg tttggtacat
    14101 cataagacca atcccagtct ttgacacggt cgttgactgg caatacttca ttacggaaca
    14161 acgttggaat cgcaaatgct tcttcaaatg catattgttg ccatttttga tatgcttctt
    14221 tttggtactc ttcatcaaat gctttgcttg aactgatatc gttgatcaat tttgtatttt
    14281 cttctgattc ataacgagtg tagttgaacg cagcatttgg tccccatagt ccacttggat
    14341 ttggatcagt tcctgtattc caagcagctt ggtaaacatc aatttcagga tcatcgtttt
    14401 ctaatttatc atagaaagaa ttgaattcga tcagacgacc tgttgtgtat tggacatcca
    14461 aaccaatttc tttccattgt tggatatagt aatctgccaa tggttgcgct gtttccccac
    14521 cggacatcga agcaaagttg attgttagtt tatttccatc tttgtcttct cttaaaccat
    14581 cgtcatccac atctttgtag ccagcatcat ctaatagttt tttcgctttg tctagatctt
    14641 gtgtgaatcc tttcatctca tcatcatggt atgactggaa tactggagga atcaatgaag
    14701 tcgcattgct tctcaaacct gcatagaaac gttcacctac tgcattatta tctaatgcat
    14761 agcccattgc ttgacgtaat gatttatcag ccatttttga atctggattg tattccacac
    14821 tctttgtatc tgcattccat ttacctaact tgaatcctag ataagtataa gaagtttgtt
    14881 cacggcctaa gtttgtatag ccagcaattt ctttataggt atcgtaagta tctgttggca
    14941 tttggaaaat catatcatat aatttagagt tcaatgcttc agttgcactt gccatcggta
    15001 cagatttcat cgtgatcttt ttcaatttag gcgtaccatt ccagtagtgc tcgtttggca
    15061 agtattctac tgattctcct gcaacgatat tactcatgta ataagggcca aaagtcactg
    15121 gatttttacg tacttgatcg cttgattcca tatctttgat tgctacacct tctaacgtat
    15181 gttttggtgc tgcatatgac cagataccac cacccgattg aagcatgctt gggtttactt
    15241 ctttatattc gatttcaact gttttgtcgt ctattttttt gattccggag attgtgtcgg
    15301 cacttccatc atgatattct tccataccta cgatgttacg gaaagtgtcg tcataacgga
    15361 taccagtgta atctttattc ccaatgattt cgtaagggaa aatcacatcg tccgccgtta
    15421 catcttcacc atctgaccat ttgacgttgt cttttacttt gattgtcgct tttttcgtat
    15481 cttcatctag atcaagcgtt gcagctccgt cgtctgtgat tctgaaatcg tcatcgtttg
    15541 caaatagcgg ttcatgagaa ggagccataa aagctgcatc atatgaatct tggtaaaact
    15601 cccactggaa caatccttta aactgactgt ccgttgcgac cgcagcttct aattcgccgt
    15661 cttcgattgc atcatcatta ttttttgttg caattggaaa agttaattcc tctttgttat
    15721 tgccgttgcc tccagagttg tttccagagg tatctgtacc tccgcccccg catgctgcaa
    15781 gaacgacagc agacatcagc gctgcaaatc caaaaatcgt tttcttcatt cctatttccc
    15841 ccttcgcttc tatactagcc taaccgttgg cgcgcatctg cagaacgctt gaatgcttgt
    15901 ccaacataat tgatactcaa catcaaaact aaaataagga ttgatgcggg taaccagatc
    15961 cattgccgat tcactaagac ttctccgtta cttgcataac cgatcaatgt acctaaacta
    16021 ggtacatttg ccggcaagcc gaaacctaag aaagtcaaag ttgtctcgat accgatgttt
    16081 gccgcaaagt tcatcgtcaa attagtaatg attaatgaac ttagattcgg catgatttcg
    16141 cggaacatga ttttaaaatc attggttccc attgtttttg aggcactgac ataatctctt
    16201 cttgcttcag ataatgtctt gcttcggaat aatcgggctt tagctaccca ataaaatgca
    16261 ctcatgatga atataaagga ccagacattg tatctggaaa taatcgatac gaagacgata
    16321 atgatcagca tgatcggtag aatcatgata aaatcaacga tccgcatcaa gatgttgtca
    16381 atgatccctc cgtagtagcc agagatgatc cctaatccaa ccccgatgat cgaagtaagg
    16441 atcgtgatcg caaatccgat cgtaacagag tttctagcac ctatgatcaa ttgtcctaat
    16501 acatcacgtc cgccttcatc tgctcccaga atgaactttt gtccttgtgt cgaaactgtt
    16561 cctggtgcag cgtatttgtc gaagatacta acatacatca ctttgtcttg atcggtcagc
    16621 atcgcaccga taaagatcac taataataaa accactagca aacctaatga aaaaatagca
    16681 attttatctt ttttaaattc tcgagcaatc attcgtatcc ccataggagg aatactttct
    16741 tgaactgctg tattgttttt ttcactcatc gatccattct cctttctgat tggttttatt
    16801 gaatccgaat acgcgggtca acgatactca tgatgatatc tgaaagtaat gtccccacta
    16861 gcgtcgcgat tcctaaaatc aatactaagg atgtgatcac ggtatagtcc cgttggccga
    16921 tggaatcaat aaatagtttt ccgactcccg gataaccaaa aatcttttcg ataactacag
    16981 aaccagcaat caacgaagtg atttcatacc ccatttgaga agccatcggc aacgctgcat
    17041 ttcggaaaat atgtcgactg aatactttat tcgtcggtac ccctttggaa cgggctgttc
    17101 tgacaaaatc gagcgatttc gaatcgatta cttcacttcg cagatattgg attgtgacgg
    17161 cagtcgccaa aaatgcttgg gtaattgatg gtaacagaat atatttcaaa cggtctatcc
    17221 attgagtgat aaatccacct tgcaaaccta gtgtttgtga tccacttgtt gggaaccagc
    17281 ctaaacgata accaaaaaca aatagcatga tcaagccgaa gataaataaa ggaacagcaa
    17341 aactaaagaa gttgtagatt acgacaatct tatccaagat tgaattttga taccggccag
    17401 caagcatccc cagaggcaca gcaatcaaat aagtaaggac aactgtcaag atcgatagat
    17461 ataaagtgtt gatgatccgt tcgccgatca agtcagcaac agagcgtttg tataagaaac
    17521 tttgaccaaa atcgccttgc aaagcatttc cgatccaacg gaagtactgg acataccatg
    17581 ggtcattcaa gccagctgct tgtcgcatct gttcgatcac tgccggatcc tgatttggat
    17641 tgatcaaacc ggtaaatgga tcccccggca tcgcttgggc taataagaag atcagaacac
    17701 ttaaaataat aacttgcggg atcatcaata aaacacgtcg cagaatcgtt ttccacatta
    17761 gcttgcacct ccatctttca acgccacttt gtgtgtagtt gtgatcgtac gcaaatcgta
    17821 gactcgacca gtcgcatcat agtattcttt ttggttttgg atatactctt gttctactct
    17881 tcgtcgattt tctttgtggc tttccctatt ttctacatcg atttgcggaa tagctgaaag
    17941 cagacgtttc gtatagatat gtcgcgggtc attataaatg tcttctcttg taccgatttc
    18001 tacaaatcga cctttgtaca taattgcgat attgtcgcac atgtgtttta ctacacctaa
    18061 atcatgtgaa atgaataaat agctgaggcc gtattcttgc tggattcgtt tcatgaaatt
    18121 caatacttgc gcttgaacag atagatccag agcagataca ggttcatcgg ctacgatcag
    18181 ttttggattg gtagcaacag cacgggctac tcctagacgt tgacgttgtc cgcctgaaaa
    18241 ttcatgagga tatttatata gcgcatcttc cggcatccca acgatatcta gcagtccttt
    18301 tacctttttc ttttcttctt gatcgcttaa ccgttcaaaa tttcggatgg gttcagcaat
    18361 gatatccaac actcgctttt taggattcaa actggacatt gaatcttgga agatcatctg
    18421 cacatccttg ttataaccca tttttttgcg gttgcttcgc ttcgttacat cttttccttc
    18481 atacatgatt tgtccggctg ttaccttttc taatcccaca acggcttttc ccgtagtaga
    18541 ttttccagag ccagactctc ccactaaacc atatgtcttt cctttttcga taataaaatc
    18601 cacaccatct acagcatata catgatccgt tactcgattg aagaatccac tgcgaatagg
    18661 gtaatgaact ttcaggtctt tgattgtgat taattctgtc atctacacct ctcctttttc
    18721 gtctctaaag tggaaatgct tatagcagga gcaacgtacc aagtggttcg gtgcgacttc
    18781 atgaaggata ggctcttctt catgatcgct tgctggtatc catggaatac gtggtgcaaa
    18841 ccgacaacct ttacgaggca tgtttttcag agaaggaacg acaccttcga tcacatggag
    18901 ttctgcatca tgatttcctt cttgtggtat agagttcagt aaagagcgtg tataaggatg
    18961 cttaggatga tcaaacagtt cttttacacc tgctacttca acgaactgtc cgccatacat
    19021 cactgccact cgatcagcca tttctgctac gactcctaag tcatgagtga tcaagatgat
    19081 ccctgttttt gtttcttcct gaaggtcttt cagtaaatca agaatctgtg cttggatggt
    19141 tacatctagg gctgtcgtcg gttcatctgc aatgacgatt ggaggtttgc aggcgattgc
    19201 gattgcgatg atgacacgtt gtctcatacc gcctgatagt tcatggggat attggcgtcc
    19261 aactctcgca ggattaggaa tccctacttg ttctagcaat tctagtgcac gagcttgacg
    19321 ctgttctgca ttcatctttg tgtgatagga aagtccttct ttgatctggt cttcgattct
    19381 catgagagga tttaatgcag acaacggatc ttggaaaatc attccgatat catttccgcg
    19441 gattttatta tagagcgttt cgttcaggtc gattaaattc agatcgttat ataaaatctc
    19501 acctgtaatt tttgtgttat taggatcaag taatcccata atcgtagtag ccaaggtact
    19561 tttcccgcac ccagattccc caacgattgc taaaatctcg tttttgtcta aagtaaatga
    19621 aacgtcatct actgcatcgt aatagtcatc tttgagtcgg aatcctgtat gtaggttctg
    19681 aacttctaat aattggttat cggacaatcg aatacccctc tctgcttcct aaatggaagt
    19741 ttttcttaat aaaaaaggac attacacaga ataaagcgtt ttataacaa
//
LOCUS       DACCZY010000052        19772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCZY010000052 DACCZY010000000
VERSION     DACCZY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.052"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2131)
                     /locus_tag="IVZ67_001502"
     CDS             complement(<1..2131)
                     /locus_tag="IVZ67_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165582.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
     gene            complement(2128..2832)
                     /locus_tag="IVZ67_001503"
     CDS             complement(2128..2832)
                     /locus_tag="IVZ67_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8165583.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2844..5153)
                     /locus_tag="IVZ67_001504"
     CDS             complement(2844..5153)
                     /locus_tag="IVZ67_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8165584.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5348..5812)
                     /locus_tag="IVZ67_001505"
     CDS             complement(5348..5812)
                     /locus_tag="IVZ67_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165585.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5812..6438)
                     /locus_tag="IVZ67_001506"
     CDS             complement(5812..6438)
                     /locus_tag="IVZ67_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8165586.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6445..6810)
                     /locus_tag="IVZ67_001507"
     CDS             complement(6445..6810)
                     /locus_tag="IVZ67_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8165587.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6800..7138)
                     /locus_tag="IVZ67_001508"
     CDS             complement(6800..7138)
                     /locus_tag="IVZ67_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165588.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7128..7454)
                     /locus_tag="IVZ67_001509"
     CDS             complement(7128..7454)
                     /locus_tag="IVZ67_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8165589.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7435..7713)
                     /locus_tag="IVZ67_001510"
     CDS             complement(7435..7713)
                     /locus_tag="IVZ67_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8165590.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7715..9079)
                     /locus_tag="IVZ67_001511"
     CDS             complement(7715..9079)
                     /locus_tag="IVZ67_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8165591.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9092..9667)
                     /locus_tag="IVZ67_001512"
     CDS             complement(9092..9667)
                     /locus_tag="IVZ67_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8165592.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9633..10820)
                     /locus_tag="IVZ67_001513"
     CDS             complement(9633..10820)
                     /locus_tag="IVZ67_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8165593.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10884..12611)
                     /locus_tag="IVZ67_001514"
     CDS             complement(10884..12611)
                     /locus_tag="IVZ67_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8165594.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12608..13060)
                     /locus_tag="IVZ67_001515"
     CDS             complement(12608..13060)
                     /locus_tag="IVZ67_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8165595.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13172..13552)
                     /locus_tag="IVZ67_001516"
     CDS             complement(13172..13552)
                     /locus_tag="IVZ67_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8165596.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13549..13935)
                     /locus_tag="IVZ67_001517"
     CDS             complement(13549..13935)
                     /locus_tag="IVZ67_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165597.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13937..14215)
                     /locus_tag="IVZ67_001518"
     CDS             complement(13937..14215)
                     /locus_tag="IVZ67_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165598.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            14263..14991
                     /locus_tag="IVZ67_001519"
     CDS             14263..14991
                     /locus_tag="IVZ67_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP8165599.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15137..15613)
                     /locus_tag="IVZ67_001520"
     CDS             complement(15137..15613)
                     /locus_tag="IVZ67_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP8165600.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15573..15920)
                     /locus_tag="IVZ67_001521"
     CDS             complement(15573..15920)
                     /locus_tag="IVZ67_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165601.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15907..16092)
                     /locus_tag="IVZ67_001522"
     CDS             complement(15907..16092)
                     /locus_tag="IVZ67_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165602.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16093..16365)
                     /locus_tag="IVZ67_001523"
     CDS             complement(16093..16365)
                     /locus_tag="IVZ67_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP8165603.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16362..16565)
                     /locus_tag="IVZ67_001524"
     CDS             complement(16362..16565)
                     /locus_tag="IVZ67_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165604.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16690..16902
                     /locus_tag="IVZ67_001525"
     CDS             16690..16902
                     /locus_tag="IVZ67_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165605.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16935..17129)
                     /locus_tag="IVZ67_001526"
     CDS             complement(16935..17129)
                     /locus_tag="IVZ67_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165606.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(17142..17324)
                     /locus_tag="IVZ67_001527"
     CDS             complement(17142..17324)
                     /locus_tag="IVZ67_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165607.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17951..18316)
                     /locus_tag="IVZ67_001528"
     CDS             complement(17951..18316)
                     /locus_tag="IVZ67_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165608.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18356..18658)
                     /locus_tag="IVZ67_001529"
     CDS             complement(18356..18658)
                     /locus_tag="IVZ67_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165609.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18658..18957)
                     /locus_tag="IVZ67_001530"
     CDS             complement(18658..18957)
                     /locus_tag="IVZ67_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165610.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(19120..19389)
                     /locus_tag="IVZ67_001531"
     CDS             complement(19120..19389)
                     /locus_tag="IVZ67_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165611.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19401..>19772)
                     /locus_tag="IVZ67_001532"
     CDS             complement(19401..>19772)
                     /locus_tag="IVZ67_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165612.1"
                     /translation="YIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDY
                     QERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAID
                     QEAYLKKKALERANRQSKAPF"
BASE COUNT         5507 a         4206 c         3066 g         6993 t
ORIGIN      
        1 ccatccattg tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg
       61 ttattggttc tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc
      121 atgttgctgg accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg
      181 gtccacctat ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc
      241 ccaatattcc acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc
      301 tccaagctct cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc
      361 ctccgccaga tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt
      421 ttccctgaat atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc
      481 ctttttctgc agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca
      541 aagaattttc ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat
      601 ctgttgttcc gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg
      661 ttagctccat gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta
      721 acacatcacc ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt
      781 tgatgaagtc agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta
      841 ctccgttgtc tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg
      901 ccacatttgg agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga
      961 catgcccacc agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg
     1021 tttctgttcg attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag
     1081 tgtacagtga taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat
     1141 aagtgtattc gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag
     1201 cgacatcacc aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt
     1261 gttccaacgc caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt
     1321 caaataaggt tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac
     1381 gtttggcaat acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga
     1441 taggttcttt gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat
     1501 tgacaattga ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa
     1561 tccgaaaacc attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat
     1621 caagttccgc gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg
     1681 cttcaactgg cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat
     1741 agttaaattt ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat
     1801 tgccgttatc cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc
     1861 gagctgttac agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc
     1921 atgatccatc tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact
     1981 gtccgcttct tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt
     2041 ctggcaaatc ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt
     2101 ttttataagc ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac
     2161 ttctttaaat tttccggata tttttacctg attccattca ggctgcaata ctggccaatc
     2221 accacgcgta aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac
     2281 gattgttgca gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat
     2341 atctcctccg ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa
     2401 aatatccata acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg
     2461 ctgacaacga aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac
     2521 ttctttatcc atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa
     2581 ttggctttta ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat
     2641 cccttctatt tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct
     2701 tcggccctga atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc
     2761 ctcaatgatg caattcatat caattactgc attccgattt ttccaaataa aatttggttc
     2821 atctggattc ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt
     2881 ttgtttgctt tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg
     2941 actttgccaa tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct
     3001 ttgcgttttt cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt
     3061 aactcaggtc cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct
     3121 aaataaggca gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt
     3181 atctttatat tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata
     3241 ggagctttcc ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct
     3301 tgccatgctt ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg
     3361 aaaattcgtt ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat
     3421 ccaccaaaat aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg
     3481 aagaaagaat tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca
     3541 gtaactaatg cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat
     3601 aaaccagcaa gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta
     3661 acagatccca taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca
     3721 gcaattgtta tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat
     3781 tcctgtaata aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca
     3841 cctacgtcag ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg
     3901 tcgagagtag catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct
     3961 aaatcttcca ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta
     4021 gtcccgaata cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc
     4081 ttgtcttgta gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact
     4141 gttgctttag ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt
     4201 ccaagtaaag tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca
     4261 tcaattgctt tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt
     4321 gttacatcaa ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt
     4381 cgagcagata ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga
     4441 taattggttg aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca
     4501 atagcttccc caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac
     4561 tgtgaaagtc tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt
     4621 gcctcgtcaa cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg
     4681 gttactccaa cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt
     4741 ttctggagtt tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc
     4801 gtctgttcta atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta
     4861 taaagttttt ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc
     4921 tctctcagca tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta
     4981 gccgctcgca atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg
     5041 tactcggcag cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg
     5101 acttcgccat tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt
     5161 agcgctcctt tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa
     5221 tcggtgtttt gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt
     5281 aaaaagaaac tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa
     5341 ggagttccta tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa
     5401 agtcttgtgg cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa
     5461 tttccataca ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa
     5521 tagtttctgg atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca
     5581 tgatttttag atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata
     5641 gcatggcgtt catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa
     5701 attccatcgt ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag
     5761 cagaattttt taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc
     5821 acctccaact ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa
     5881 tttattaaat aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac
     5941 attattgtat ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc
     6001 ttcctctgca gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca
     6061 aactgctttt ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa
     6121 ttctcgtgcg gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc
     6181 atccatcact tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg
     6241 cgacacacga cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt
     6301 gacaccgatt tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc
     6361 aacagaatct ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa
     6421 agtatcttgt tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat
     6481 aatttcttca attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg
     6541 ttgatttgtt gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac
     6601 tttcacttga tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa
     6661 gagcgactta ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa
     6721 agattccaac gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat
     6781 ttcagaaaaa ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt
     6841 tggttacggt atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc
     6901 cagcattttg cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa
     6961 aagagacttg gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt
     7021 tactcacatt tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga
     7081 acactgctaa ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa
     7141 gctaaactca actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac
     7201 gttacagaat caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact
     7261 ttaattcgtc tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc
     7321 acaccgagtt cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg
     7381 tttccgtctg ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc
     7441 tgtctcttcg gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc
     7501 tttgtcgagt tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat
     7561 aagatattct tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata
     7621 aaaagagagc aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc
     7681 ctctaaagac aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt
     7741 cgtagcgata tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt
     7801 aaatttggaa tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca
     7861 aggcaccaat aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg
     7921 tgtctggttc acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt
     7981 ggtaaccaat tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg
     8041 ttttcatttt ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca
     8101 tcacttctgt cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag
     8161 cacctgaagc agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac
     8221 caggattttc atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat
     8281 aagctttttt caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata
     8341 attttttagt tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa
     8401 actgaatagc tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg
     8461 ctttcttcac aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt
     8521 tacgaagtaa attttcttct tgagcataag taataacttc agatgcaatt acttctggga
     8581 cagtaactga accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat
     8641 ttccagctac aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca
     8701 tttttgcacg tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt
     8761 gagtaatcat tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat
     8821 cgttgtcttc accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat
     8881 tgtcgtctgt ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt
     8941 catcaataac actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc
     9001 ctgattcaac tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt
     9061 tcaataattc tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt
     9121 tgattaattt ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag
     9181 cttctgtatc ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat
     9241 ggattgttcg aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac
     9301 caaaactaca ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg
     9361 ttgtgtccgg taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg
     9421 ttccgctttt agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat
     9481 ccgaattact aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc
     9541 ataaaagtgt ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt
     9601 cttcatcgga acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca
     9661 tttccatcac ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt
     9721 ttctggccaa tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc
     9781 tgtttaggaa aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg
     9841 atgttgtagc caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc
     9901 cattccactc tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg
     9961 tttttcaata ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct
    10021 gtcttcatca gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt
    10081 ttgtaaacac ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg
    10141 atggaatatc cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt
    10201 ccttccagtt gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg
    10261 atgtgggcgt ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc
    10321 aaaacttttt cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca
    10381 gcacctttta atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt
    10441 ttttgaccat tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg
    10501 tacgccaaat gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa
    10561 gtatttgttt ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa
    10621 atatttagta agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg
    10681 gccaatgcca cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg
    10741 atagatgaat ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg
    10801 atatatccac gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc
    10861 aatttttttc accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca
    10921 tctgaatcaa tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg
    10981 aacccatcaa tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta
    11041 gtaattgcta caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt
    11101 ttctcataaa acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga
    11161 gcaacttcaa cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc
    11221 gctggatcat aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc
    11281 aaaatctgat cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc
    11341 aaatcactgt aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata
    11401 aaataaagtt gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt
    11461 aaagatacac aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg
    11521 ccacttaaat caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca
    11581 aagaaattat ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg
    11641 gaatgagctg accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat
    11701 attccccagt taggattgca catttcccag acttttctat ccgtccaatc ataattttta
    11761 tttggctcat agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt
    11821 gcttcgcgat atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta
    11881 aacatcaatg gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt
    11941 ttctcctgtt tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta
    12001 tcaatattct tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag
    12061 tcataagtaa tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt
    12121 aggattggat tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca
    12181 aaatcagatg cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat
    12241 cctaatgaac cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc
    12301 tcacgagtaa cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa
    12361 gaataaataa atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt
    12421 gtgtgaacaa taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac
    12481 caaatatctt ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta
    12541 ggatacttct taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca
    12601 ggttcaatca tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt
    12661 catctactgg ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc
    12721 cagcaattag tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca
    12781 tcttcttatc tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag
    12841 ctcctgattt attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat
    12901 atttgtgttg gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga
    12961 tggtgtacaa atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt
    13021 tttcatcaat aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt
    13081 gttttttgaa aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg
    13141 cgaaaaaatt ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt
    13201 tgtcttccgt ttcgttttca acaattggat gacattttga acataatagc atgagattgt
    13261 ttggatcaag cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc
    13321 tctttccaaa tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa
    13381 attgacgcat atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa
    13441 cagactttgc ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt
    13501 agataccctt tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc
    13561 acgaataaga tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg
    13621 tttcttagca tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc
    13681 agtggactga tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct
    13741 atcaagaatt tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc
    13801 atcactcaaa attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac
    13861 aactactgtc cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat
    13921 tttgatcatt gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca
    13981 ttcgctcata ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa
    14041 taactaacgt attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat
    14101 taccattcca attttctctg tacggactat tctctgcttc aattgctttc ttataaactt
    14161 tagccaaact tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt
    14221 tactctccta tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg
    14281 cttcaagaag ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca
    14341 aatagttgtc cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca
    14401 tatagtgcgt tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag
    14461 tgcaacaaat tttatagttt acaatttata aatacgttta atgaagatga aggatattct
    14521 gaaaatattt taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat
    14581 attgaaaaag tttctcctac atttgttgat atttatactc aagccacaca agctaaatct
    14641 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
    14701 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
    14761 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
    14821 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
    14881 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
    14941 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
    15001 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
    15061 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
    15121 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
    15181 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
    15241 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
    15301 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
    15361 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
    15421 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
    15481 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
    15541 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
    15601 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
    15661 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
    15721 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
    15781 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
    15841 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
    15901 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
    15961 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
    16021 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
    16081 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
    16141 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
    16201 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
    16261 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
    16321 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
    16381 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
    16441 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
    16501 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
    16561 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
    16621 cttttttatt tgatataata cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg
    16681 tgatttatca tgaatatcaa caatttagta aaactcgttg aacaacgttt taaagaatca
    16741 ggattagaac aattaaaaaa agataattct ccagaattat tttctaattt taccgaagac
    16801 gacattccta ctcttaaaca attaaaatat tattctgatt attggacgca aactcgtgag
    16861 aatgaattaa atttagaaaa actcattatt aaaaagaaat agcaactcca gcccacacgg
    16921 ctggtttttt agtttcaact tccctcctta cgggtcaaaa tcatcggttc cggcataatc
    16981 tgatcatcct ttagcaacaa atcaggatat tttttcctta gcttcttatt gttcgtgtaa
    17041 gttttcaata gccattggtt ggcttcgctt ttgctttctc cagaaaaata aattttccct
    17101 gtttggatgc ctgcaagttg aatcatcatt ttcacctcaa atcaaatttt ttctgctaga
    17161 tggagcaaaa gcaatccgac atcaagaatc ttttgaccca ttatttcagc tactttgctc
    17221 ggcttaactc cttgagaaaa gagcaagcgt gcttgctcta tctcttcgtg agtccaaata
    17281 aatttggctt cctcaaggat aattactgga tcagttgctc tcattgttcc tctggaaaaa
    17341 tgatgtcatt cttatgcttg gaccaggtat ctgcatatgg aacaaagtat tgtctagcta
    17401 attcgatttg atcaattaaa gcagtttctg ataattcatg gtctgcagca atttcattca
    17461 tactatcgcc ttcatctaaa cagataagac cgttgacgtt atcgattttt acatttgtga
    17521 ctgcttgact ttctccacgc tcataatatt ctacagttga tactgataca tcagcttcgg
    17581 agtttaattg cagaatctgt tactgccggg ataatttaat attcttttca taattcacct
    17641 tttttcctac tgacatcgaa tatttttcat gttatgatta gccgtgaaag gagagctata
    17701 ttatgtgttt acacatgatg tccttagctc attcggttct ttggaagctt ttttggaaat
    17761 aactctcagg ttcttgacgc aaatctcatt tattctttga aacaaaaaac ttccattttc
    17821 cgagagcttt ggatcacttc aaaagagatt tgcatttata tttctgtgac cttgagtcgc
    17881 gtaaagaacc ttacagcaat ggcggcaatt ccactttcag gaaaccgcct tttttcattg
    17941 atattggtgg ttagcggact acccattttc tttcggcagt gccaaaccac aaatgatctg
    18001 tgttgatgtt taggtagttg tataccagat aagctaaatc caaagtgcct gcatcttcaa
    18061 gacgacaata ttccaaaaat tctcccatag ctggacataa aaatcctgga gctatcatat
    18121 cccaacagtt aatagtgcaa agaaattcgt ttagcttctg aacgttttct ttggtttcac
    18181 gaattacaac atactccaat tcttctccgc tggcagagca ttccaaaact tcaaattgat
    18241 taagaaatgg tagaacatat gcttcaaaca tcaacattcc atctttctgt ttttgcactt
    18301 gttttgcatc aatcatgtct atcctcctat tcagttattt cttccattaa tggaattatt
    18361 tcaggtcact ggacttaacg aaaacaccat tgaccatctt tccagtccga cctttgattt
    18421 cgtcataagc aaaatccaag cactcgtata aatccatatc attttgcatt gccaagatga
    18481 tcaaggttac tactacatcg ccaattccgt ctcttaaacc atgttcatct tttctagcta
    18541 gagaagcggc aacttccccg atttcttcga ccgtttttaa catttgcttg ctgaaatccg
    18601 cttgatccaa tcccttatct tttgcccact gctctacttt cgcgattagt tcgtccatta
    18661 tttctcctcc acatacctaa actgtcttcc ttttgaatca atccataagc tcctagctct
    18721 atcccaaata atatttttgc tcagaccagt aatttcagat aactgtttag cggtacccgt
    18781 tactagaatt cgatcaccat gccagattgc aatctttctc ggcgttctcc gtttgggctt
    18841 ttcagtccac attgatttac cgagcttttg gacttctgca actatttttt tatcttcctg
    18901 ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct tatccatccc
    18961 gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt ccgccatgaa
    19021 atgtgttgag ccacttgtct tcgtacgaca cactttgtct taaagcttct tgatgcatta
    19081 attcgatctg tgctgtaaat gtctttaggt ccatcttgtt tacacctgct caagttcatt
    19141 aagatgtttt tgcaagcctt taacacaatc aatgaatagt aatttggtat atgctaaatt
    19201 tcttaattgc gttccatcga tatagagtgc gaaatagtat ctgagtttac tccaactcga
    19261 acgatcattc ttaattcctt caattccagc ttcttcgagt tgatcatata cgtctctcag
    19321 gacgtctaat tcctcgccct ttttataatt cgctatttca ttaattagtt ctagataatc
    19381 gatcttcatt tttccacctc ttagaatggt gcttttgatt gtctattagc tcgttctagc
    19441 gcttttttct tgagataggc ttcttggtcg attgcccatt caggaagctt ctctcgtctt
    19501 cctgtgcgct tgtatccact gcttgcgttc ttaggttcac ttttttcttt ccttgcccaa
    19561 cttcgaatag ttgccaaata gtttttataa gtcttaccag atgattcaca atactctgac
    19621 agtcgttcga ttcgctcttg gtaatcatta gggaattctg ttttgagttt ctccatctgc
    19681 tcatctgaca aaagaacatt tttatactct ccgtatttat gacggacggg cttagccttc
    19741 gattttttcg aaggcggtaa ctctcttata ta
//
LOCUS       DACCZY010000053        19398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCZY010000053 DACCZY010000000
VERSION     DACCZY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.053"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVZ67_001533"
     CDS             352..1041
                     /locus_tag="IVZ67_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8165613.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVZ67_001534"
     CDS             1028..2236
                     /locus_tag="IVZ67_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8165614.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVZ67_001535"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVZ67_001535"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8165615.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVZ67_001536"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVZ67_001536"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8165616.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVZ67_001537"
     CDS             complement(6339..7034)
                     /locus_tag="IVZ67_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8165617.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVZ67_001538"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVZ67_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8165618.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVZ67_001539"
     CDS             complement(8233..8424)
                     /locus_tag="IVZ67_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8165619.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVZ67_001540"
     CDS             complement(8445..9335)
                     /locus_tag="IVZ67_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8165620.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVZ67_001541"
     CDS             complement(9322..10089)
                     /locus_tag="IVZ67_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8165621.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVZ67_001542"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVZ67_001542"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8165622.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVZ67_001543"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVZ67_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8165623.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVZ67_001544"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVZ67_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8165624.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVZ67_001545"
     CDS             14705..15043
                     /locus_tag="IVZ67_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165625.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVZ67_001546"
     CDS             complement(15211..15969)
                     /locus_tag="IVZ67_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8165626.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVZ67_001547"
     CDS             complement(15987..16805)
                     /locus_tag="IVZ67_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8165627.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVZ67_001548"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVZ67_001548"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8165628.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVZ67_001549"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVZ67_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8165629.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVZ67_001550"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVZ67_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8165630.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5501 a         3988 c         3572 g         6337 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacat
//
LOCUS       DACCZY010000054        17999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCZY010000054 DACCZY010000000
VERSION     DACCZY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.054"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            93..1433
                     /locus_tag="IVZ67_001551"
     CDS             93..1433
                     /locus_tag="IVZ67_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165631.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1651..2094
                     /locus_tag="IVZ67_001552"
     CDS             1651..2094
                     /locus_tag="IVZ67_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8165632.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2598..4016)
                     /gene="nhaC"
                     /locus_tag="IVZ67_001553"
     CDS             complement(2598..4016)
                     /gene="nhaC"
                     /locus_tag="IVZ67_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8165633.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4419..5642)
                     /locus_tag="IVZ67_001554"
     CDS             complement(4419..5642)
                     /locus_tag="IVZ67_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8165634.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5673..6512)
                     /locus_tag="IVZ67_001555"
     CDS             complement(5673..6512)
                     /locus_tag="IVZ67_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8165635.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6529..7209)
                     /locus_tag="IVZ67_001556"
     CDS             complement(6529..7209)
                     /locus_tag="IVZ67_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165636.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7211..8284)
                     /locus_tag="IVZ67_001557"
     CDS             complement(7211..8284)
                     /locus_tag="IVZ67_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165637.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8931..9602)
                     /locus_tag="IVZ67_001558"
     CDS             complement(8931..9602)
                     /locus_tag="IVZ67_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8165638.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9689..12367)
                     /gene="mgtA"
                     /locus_tag="IVZ67_001559"
     CDS             complement(9689..12367)
                     /gene="mgtA"
                     /locus_tag="IVZ67_001559"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8165639.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13026..13463
                     /gene="ndk"
                     /locus_tag="IVZ67_001560"
     CDS             13026..13463
                     /gene="ndk"
                     /locus_tag="IVZ67_001560"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8165640.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13485..14351)
                     /locus_tag="IVZ67_001561"
     CDS             complement(13485..14351)
                     /locus_tag="IVZ67_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8165641.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14511..16604)
                     /locus_tag="IVZ67_001562"
     CDS             complement(14511..16604)
                     /locus_tag="IVZ67_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8165642.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17353..17976)
                     /locus_tag="IVZ67_001563"
     CDS             complement(17353..17976)
                     /locus_tag="IVZ67_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165643.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5177 a         3724 c         3169 g         5929 t
ORIGIN      
        1 tctgaaaatt tgttatcatt agtcattgtt aaatattgct atttattctt aatataaatt
       61 ttgattgtga atttaaaaga aagaggtcat ttatggaaga taaaaaattg aaaaaacaac
      121 ttagctccag acatatcacc atgttagctt taggtggagc aatcggtgca ggtctattta
      181 aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt attgattgct tttttgattg
      241 gtgggctgat tttatatatc gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg
      301 acacgcatca cggattatct ggtttgatac gcccttatct aggtgctcat tctgctgatt
      361 ttactgattg ggtctactgg tctatgtgga ttatcaatat catcgctgaa gctgttgcag
      421 ctgcttcctt tttgcagttg tggttcccgc acattccagc ctgggtattt gtttttatgt
      481 tggctgtttt aactacgatc atcaatcttt attcggtacg gttattcgca gaaacagaat
      541 attggctggc ttttgctaaa atcagtgtca ttatcttact gatcattttt gctacttatc
      601 ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac ccttcagcaa ttgactgacc
      661 atggtgggtt tactcctcat ggtatgaagg gaatcatcag ttctttgctt gtagttatct
      721 attcttacgg cggttctgaa ctgattgcta tcacggtaag cgaagcagat gatcctaaac
      781 gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat catttccttt tacatcattc
      841 ctttgttttt gcttttgatt ctttttcctt ggaatacttt agcaggcacg aatgtcagtc
      901 cttttgtgat ggtatttgaa aaaatgaata tcccatttgc agcggatatc gtcaattttg
      961 tgattgtcct tgctttgttc tcatctatca attcgggtgt gtatgcatct tcaagaattt
     1021 tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc
     1081 atcaagtacc gcaacgtgct gttttatttt gtaccagtgt tctttatctt ggtgtagctc
     1141 tttcttattt tgtcggtgac aaattatttg gctatctggc aggttccctt tcgtatacag
     1201 tattattgat ctggatcatt atcagtgcgg catcctttgt actggcacta agaaaaggaa
     1261 cattatggga taaaggaatc agcttttttg cattagctgt cttaggattg attttccttg
     1321 gtatcctgtt cacgaactcc attggggtaa ctgtattgac tggcctgctt tacctgttca
     1381 tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat
     1441 agaaaatttt attttattta tgtggaatag atggagctat tgaagaaagg agttgtgaca
     1501 aaagtcttgt tgcaactcct ttttccgaat aaacagtctc atgacctctt ggtaaacggt
     1561 gttcaaaaac cctctgtgca aaataattga ttgttctcca tgctcatgac atgatagaag
     1621 aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt attggaagtt tttgttgatg
     1681 atcggtgttt caacggtagt tatgtttggt atgatgtact tgaatgtcta tgcgctcgat
     1741 catatcttct tcagtcgtac acgagtattc atggcgttga tgatgggtgc agtcatggca
     1801 gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc
     1861 ttagccgcta gtcttcttgt ttttgccggt tccttgttta tggtacgaag ccaaactact
     1921 gtcaacgaca cagcttggat gaaggccatg attcctcatc attctatcgc tatcctggca
     1981 agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga aatcattcag
     2041 gcacaagaag aagagatcga caaaatgaaa aaaatgatca aagaaatgga atgaccatta
     2101 tccgtccaaa taaaaaaaga ggatgttcca gaagttatca gcttcaagaa ataagaagaa
     2161 gctacttctg gaacgccgtg aataaggggt tgtgaaaaag ctgccctgca tcaagtaata
     2221 agaacagcca aacaaaaata gcttctccta tttttcgttt ggttaggctt attaccgcag
     2281 atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa tcagcgttct gacctgagta
     2341 ttagagagac aaatggaaaa atagttcctc atattttgcc attttttgag ctaataccga
     2401 gggtcagctg attgaacacc gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt
     2461 attaagaaaa attttgtgaa atggctttcc acatttttgt ataaatttgg cttaataccg
     2521 agaaactggc ttttgaatac cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac
     2581 aatccctttt tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt
     2641 ttcacaaatt tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga
     2701 gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt
     2761 gctacgaaac ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct
     2821 tctagcgttc tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat
     2881 gcattaccag gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata
     2941 cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg tcatctttct tgatactcgt
     3001 tccatcacgg ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga
     3061 ataatcaatg acacagtagg catcatactt tcgattcctc cgcggctaag cagtgaatcg
     3121 atttgttggt ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt
     3181 aaggaagttt gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa
     3241 atcgttaaaa ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc
     3301 agtgaccaga ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat
     3361 gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca
     3421 aatgccggga cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca
     3481 atagctgctg ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca
     3541 cctgaaacaa tagctccaac aaccagcgct ggatttaagc ctagtgttgt cccaataccg
     3601 aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg
     3661 attgcacaca caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca
     3721 atgaccatta gagttggaat gatccctgct tgtatccata cagcaatcaa tgaaccgact
     3781 aaaataaaaa taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc
     3841 agccaatctg tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct
     3901 aagatcgtga tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca
     3961 ataaccatta atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata
     4021 atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc
     4081 cgcaattcat ccgcgattcc tcctttataa aaataaaaag acctgtttta aactaaggac
     4141 gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt
     4201 attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac
     4261 caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag
     4321 tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa
     4381 attattttta taactttcac acacaagttt attttttact agaaagtagc gccctcttgt
     4441 ttttttcttg ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct
     4501 ttcaacagat caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg
     4561 cctttgtcac ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca
     4621 gagattgcta ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa
     4681 gaaccgagac ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt
     4741 gcatgctgga agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt
     4801 tggaacaatc caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt
     4861 tccacttttt catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt
     4921 ccagcagcgt aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg
     4981 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc
     5041 aatgcttcca aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg
     5101 taaaatttga tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa
     5161 gctggatgga cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg
     5221 cctttagctg atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct
     5281 ccaccagttt catcaatgaa ttgcttcact atgctggcgg ctaatacgga gtaagtacca
     5341 aacaaattat gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag
     5401 gcaattgtgg gaccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca
     5461 gctacttctt tttctaccca aaacctttct ttttctaatt gtttaatcaa cgctgctgag
     5521 gaataaaact catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga
     5581 tattctggta aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc
     5641 ataaaaaatt cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca
     5701 ggcacgcttg atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa
     5761 gcgtctacta ttttttgata cgttttattg ttttcatctt cttttcttgc aacgatgata
     5821 ttcacataag gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga
     5881 ttaaatccag catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa
     5941 gcacgtgctg tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta
     6001 atatcagaaa cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt
     6061 tgcaataaca gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct
     6121 ccgtctttga tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg
     6181 acagtatttc cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag
     6241 aattgatgtt ggaaagaatt caaatcaatc gatccatctg ctaaagccac atttggttga
     6301 ttgtaatctg taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc
     6361 acgttgtccc atacatctgt atcttctcca ataatcccta gttttacgat agtttctttt
     6421 ttgtcagttg tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat
     6481 gtaatgacta caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat
     6541 ttttttaatc agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa
     6601 tgtagccaca atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt
     6661 ccctaatccg cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat
     6721 agtcaacgcc gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat
     6781 gataccgatt ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc
     6841 tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac
     6901 taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag
     6961 agaaagcaag ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc
     7021 aaatagcaga cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa
     7081 gatgattcca atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt
     7141 cgtcgcttcg acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa
     7201 atcagtcatt ttatcttcct ccttcgatca ctttgatacg actatctaaa tcgttttttt
     7261 gtaaaatatt tatttttaca ccagatttga tcaaataatc caatgctttt ttccgctgtt
     7321 tggtttctcc agctaaagta acgatcaaac tccctaacgg cgtttcttgt aataactcta
     7381 cattgccata taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag
     7441 gttcgttcgt ctgatttcca atataactta attccaccag ccatttattg gcgatctgat
     7501 cggcaaaagc agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc
     7561 gaataaaatc tttggtcaat tcttcttcag gattgctgaa gatctggatg ctgctacctt
     7621 tttcaacgat tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt
     7681 gcatctcatg agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca
     7741 atgcaaggat ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata
     7801 ataccttagg ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg
     7861 acagttgttt aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat
     7921 tcactttttg acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt
     7981 tgtcaaaaat agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct
     8041 ttctttcttt tcgcaattct ttgggagaga gctgactcag attcgtctga ttgacaatga
     8101 cttctcctgc tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg
     8161 cacctgagta accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat
     8221 ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa
     8281 tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc
     8341 ttgaggacgc ttttttgcgt gttaccacct catgttcaag ctgtgttcac acacaactcc
     8401 tcttcaagta cgatcatact ctagcaagat atcggtcgca gccgtcatcg ccttacatct
     8461 atgctcggga tgaattgctc gatggccatt ttccaatagt cgttccttgc ttcttctcag
     8521 ctaccgaagc tctctgtcaa aggttcttct atcgtactta ccatttcaat gcaaataata
     8581 ttcaaaaaaa aagtcctttt actaacataa actatgtcag taaaaggacg acgtatcgcg
     8641 gtaccacctt ttttcggaag tttccttccc tcaagcccaa catctcaaaa agagagattg
     8701 gcgttcgttt taacggtcga acagttccga atttgcctac ttatcgacaa attattctcc
     8761 aaggccattt tcagcttttc ttacttgact cttctcacca gccagagcct ctctgcaaag
     8821 tttccaaact tactttcctt ttcaacgaaa tcgatatttt gttgttggga ttaatctaca
     8881 tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt ttttctatat ttatttttct
     8941 gtttttttat ttaacatttc aataacagtt cgaagatctt ttccttccgc aagaagtttc
     9001 aatttttcct tttcttcgac ggtataattt cggacaaaac tggaatcagg tatcaaaaca
     9061 acgcctaatc ctgctcgtga agcagctgtt gctcctacta aagaatcttc cactgcaaag
     9121 gctgaactag cttctaatcc tgttcgttcc aaagctgtca gataaggaat cggtgaaggc
     9181 ttgttctccg acacttcgtc tccgaatgta aacgtgtcca agtaatcagc taatttaaag
     9241 tgatccaaga tatctgttgc tcttttcgta acagtggtcg tagcaatgcc tagtttcata
     9301 tgacgttctt ttaagtctgc caatatttct tttgcatatg gttttaagtg gatggctccg
     9361 cttgtcagct gttgataaaa atattcttca cgcttcgctc ggacttcctg tactttttca
     9421 gggctacctg ctgctttcac taaaatatca aaactttgtt tccagttttt tccgccccaa
     9481 gcgtcaacga tttcttttgg gatatggata tcaaattgct gaaatcccca aagccagcca
     9541 tcacgatacg ttttttctgt gtcaactaac aaaccatcta aatcaaatat tgcacctttc
     9601 atctatacat taatcctttc caaaacaata cacatagttt agcacgactg gtccaaaaag
     9661 aacagaagca aagaaccagc cttctcgttt ataagagttc ttggaatcga cggatataga
     9721 ttttcttcac agctgtcaca agcgccagat aagcgatgac tgtagcaaat aaccataacc
     9781 agtaattgcc aggaagtggc atcaagccta attcagcacc aaaacttgta aaaggaagga
     9841 cagaaccgac aataattcct aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt
     9901 ggataaatgg tactttcggt gtacgcagtg catggagcac taaggtttgt gtccatagtg
     9961 attcgacaaa ccatcctgca tggaataatg cgatgaaaat cactcgctgt gaactatcca
    10021 atgtatggaa acttccgccc actacagcag gacaaataac aaaatacatc aacaagtaag
    10081 tagtaatatc aaatatcgaa cttgttggac caaagtaaac catgaattcc ccaatactag
    10141 atgcattcca tttttttggc tcgtccaaat attctttatc catccgatcc catggcaagg
    10201 aaatgcagga aacatcatag atcagattca aaaacaaaag ttgtaaaggc agcattggta
    10261 aaaatggcag gaacgtactt gcaatcagca cggaaaacat attgccaaaa ttagaacttg
    10321 ctgtagcctt gatatatttc atgatattcc caaaagtttc tcgtccagaa agaaggcctc
    10381 tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat
    10441 ccacagctgt atctacagaa ataccgatat cagccacttt cattgcagga gcgtcattga
    10501 taccatctcc taaaaatccg acagtatgac catttttacg taaagctgtc gtcaaacgtg
    10561 ctttttgctg aggattcaat tttacgaata cttcatggtc ttcagctact tgtgctaatt
    10621 catgatcact catttccgag attttctcac cggtaaccaa ttcattgatc tctagaccta
    10681 cttgacggca cactgaacgt gtaactaatt cgttgtcccc agtcagaact tttacagcta
    10741 caccatgttc tttcagagct ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga
    10801 aagcaagata accaatcaaa accatgtctg actcatcttt gactgaaaat tctccaacga
    10861 cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa gccatcttca ttaagtgact
    10921 gtacttttgc aagtacggct tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa
    10981 gatcaataaa tttagaaatg ttcagcattt cttcaatcgc gccttttgtg atcatttgtg
    11041 ttttaccagc tggatcagca accacaacac tcatacgacg acgttcaaaa tcaaaaggaa
    11101 tttcatccac tttttgataa ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa
    11161 tgatagccac atctaacaaa tttttcagcc ctgtttgata gtagctattt agatacgcat
    11221 ggcgcaaaac acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt
    11281 tatcttgtgt caatgtcccc gttttatccg tacataacac atcgattgca ccaaagttct
    11341 ggatggcatt caaatttttg atcactgttc cttttttggc catcacactt gctcctttga
    11401 ccaaattcgt cgtaacgatc attggaagca tttcaggtgt tagaccaact gctacagata
    11461 gtccaaaaag aaaagcttct agccaatccc cttttgtaag cccattgatg acgataactg
    11521 ctggagccat caatgacata aatttgatca gtagcatgga tgttttatgg atgcctaatt
    11581 caaagcttga ttgaattggt ttttcagata gcgtttctgc tacatgacca aatagtgttt
    11641 catttccaac agaaacaatg attccttccg cacttccact gatgacatta cttcccataa
    11701 agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct
    11761 tttctactgg atagctttcc cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg
    11821 acaataaacg catatccgct ggtatcatat cccctgctga aagccgaaca agatctccac
    11881 agacgatttc ttccatagga atctctgtgt actttccatt acgttttaca gctgccgtta
    11941 ctttcacaag tgatttcagt ttttcagccg cttgattgga acgtacggac tgaatcaacg
    12001 tcattgtccc gctgacgata accatagtga agataatggc tacaccgaaa agatctttat
    12061 ctccaggagc tgctaataca tattccgtga tgaatgaaat aattcctaat gcgatcaaca
    12121 ctaatgtaaa tggcgtgatg tatgctttca atatttctac aatgaatggt gtttttttgc
    12181 catatgaaat cgcattttca ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca
    12241 atccatttgc tgatgcatgg aatagttcaa ataattcttg tttcgttttt ccagcaaaag
    12301 ctgcatagat tttttgtgta tctttctttt tttccatctc aatctctcct ttgtgtccaa
    12361 aagacagctg ttctcgctgc ttttggtctt tagctattca tcggatcgat agagaaaaaa
    12421 ctgaaagggg ctgagaacag atagataata gccctggcct ttcctctaga tccttgtttt
    12481 tcgaatgact gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact
    12541 tccaatcatt tttcgaatag ttatttttcc aattagacca cctccttttt atttttcacc
    12601 gatggggatt tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa
    12661 agcatattaa aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa
    12721 aagcagatac ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta
    12781 cccattggcg tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct
    12841 aacaaatctt attcggtttt ccttcgttaa tataacaact aaaaaagagg atgtcaaatc
    12901 aattttccat gtttttcata ctttttttaa aatacacacg ttatattcct gtctcacgcg
    12961 gttttatttg tctcattttc cacctcccgc tataatagaa atatagtagg aaacgaggtg
    13021 ctgttatgga aagaacactt gttattatca agccagacgg cgtgagacgc catttagtcg
    13081 gaagcatcat ccaacgtttt gaagcaaagg gactggccat tgctgaaatg aagtttgaaa
    13141 ctatgactcc tgaattagca aaagaacatt atcagcactt gactgagcgc agtttttttg
    13201 atgaactgat cgattacatg acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg
    13261 aagtgatcga tatcgtccgt aagatggttg gagcaacaaa agctgccgat gctgttccag
    13321 gtacgatccg aggagactac gcgctgccgg ggactgaaaa catcattcac gcttctgatt
    13381 cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc gccattcgat ataaacaaac
    13441 cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc tggtctagtt gctgggttca
    13501 gcaatggcca gcagtttttt ctctaaggac ctccgttgat tctttcctaa catatattcg
    13561 agcactgtga agaattcttg gtagtctttc gtcttttctt cccctaaagc agcagaaatc
    13621 aatctttgat aataatctgc catcaatgtt tcatagatca ctgtacttct ttctttatgt
    13681 gataagctcc gtttcatatc ttcattcaca ctacgtgcct ttttcaaccg accattttct
    13741 atataaaacg aagtcaggtt cattaaaaaa gtaaagagat actgctcatg attgttcaac
    13801 tgtgctaatc gtcgcattct ttttttgatt ttcaaataat tgttctcaat gaaccgatca
    13861 ctgaatatcc aaggaaaatc agctagaata gatagttcaa attgaccata gtctggtatc
    13921 tgcatcaagt attcttcaat gattcggcat tcataacgta aaaattcaaa ttctgtctca
    13981 gatacttctg ttttttcgac catgtgaaga ttcaaaacca aataatacag atcttctgtt
    14041 ttcttaaaat gcctacggtt attgctgatc tctttataag gatcaaagct gtcttcttgt
    14101 acccgatcta gcattcgtct ttttttcttt aactttattg gttcatccag ctggtttcgc
    14161 atccattcat attctgcaag tgtcatgttc attcgctcta aaaatgatct caatgttaca
    14221 aatgacattt ttttcactga atttgtctca atcccgacta gagaagtctt aaaacagatt
    14281 ccttccgcta attgggactg tgtcaacttc ttgttgactc gaagtttctt gatcaattca
    14341 ccatcataca taatagttct cctttttaat ttaaattttt ccacattcaa taaatagata
    14401 cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca
    14461 accttcaagc attagaatgt tgtctaacta ctgaaggttg gtttgattgt ttattgatta
    14521 tttttttgct gttcaacgtc tgttcttgtt actggatata agttggttgt tgttttatta
    14581 ccattttttg aaaagacact tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta
    14641 cgttccgctt catttttata gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc
    14701 acataaaacc gcaagagatt ttcctgattc agcttatctg aaagacttaa cgcagtcttg
    14761 actttttcct gatctttgga gatttcctca tcgattcctg ctgctttcgt atcgatgatt
    14821 tctcctgtct gattattgta agcttttcct cctaggacgg tgtatttggg tgtcacaaag
    14881 ttcccatttc gaaaagcaac tacttgatca tgctgagaag ataataagtc tgtaccaaaa
    14941 tgaagataat ctttatcatc gattcccaat agatgtagta atgtcggcaa aacatcgatt
    15001 tctccaccgt attcatgatt gactgtccct ttagtataac caggcatatg gatcatcaat
    15061 ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg tatctggatc tttaccaaga
    15121 gcgcttgcta aatctttatt atctgtattc gagatcccaa aatgatcccc gtaaatcaca
    15181 aagattgaat tttggtaaat accagatttt ttcaaatacg taaaaaattg ttccaatgat
    15241 tgatccagat aatgtgccgt tcgaacataa ttatctactg tgctattgcc agtattcaaa
    15301 gatggaaagt cgaaattctt gtcatccgta taataaggcg tgtggttggt tacggaaaga
    15361 aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt gggcagattc tctgaataaa
    15421 tctttatcta atatcccata acctaacgtt tcgtcggatt catcaaaata agatcgatca
    15481 aagaaattgt cataacctaa gtttttatat acatgatctc tattccaaaa actagctaca
    15541 ttcccatgga aaacagcgct tgtatagcct tgcttttgtc caaggatcgc tggtgcggct
    15601 tgaaagacat tgtctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg
    15661 ccagtttcca acatattttc agcatcactt gtctttccct gtcctacttc atgaaagaaa
    15721 ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg tgtgacttct
    15781 tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac tttctaaatg gagaacgatc
    15841 acatttttcc ctttagcgat cccaaatgtt tcgggatttg gctctgcata atgtttcttt
    15901 gtataatcta aaacttcatc aatcccatta ctgcttgcat gcgcgcgaac actgctagtc
    15961 attcctgttt tgattccgtc ataaatagta tacgcatcga ttcctagata tttgacaata
    16021 tatgaacggt caaatgttcg ctgcagcaat tgtggacggt tggcttcgct cagcgctaga
    16081 tttcctgaaa aaataagccc tgaaagacag gtaacagcga tagccatcgg tttgccgaca
    16141 gggttgcttt tgataggtat tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca
    16201 gcgatatcca gccagtagat gacatcatgg ggcttcatca aagaaaagga gcttcctgac
    16261 aatccttgcg acactttcga aaagcctaat acagatttga ctgtaatgaa atctgtgaat
    16321 tcccgataaa aaataatatt caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa
    16381 agaatagcta acgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag
    16441 gtagtcgcaa ttgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaat
    16501 gaaaaatctg catagtaggc aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa
    16561 acgattgcta aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc
    16621 tattcacatt ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat
    16681 cttaagattt ttttattctt tctaaagatt attccgtcca cttttcacag cataaaaccg
    16741 ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc
    16801 cacggtattc aaaagccagt ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt
    16861 cttgtcgcag ctccttattt cttgatgcag gacagctttt tcacaacttc ttccttaaac
    16921 ggtgttctcc tgccaactcc tcggccacaa gtcacaatat ttataaaaca tgagaagctg
    16981 ttttttgaaa ttgttccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    17041 acggtgttca atcagctgac cctcggtatt agattatgtc aggacgctaa ttttacaacc
    17101 tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    17161 tataaggagc tattttttga aattgctcct tgtggctcag agttaaacgg caggctcaag
    17221 acctcttgat acacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa
    17281 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
    17341 aacctctgtt tttcattgtc cataggattg atattgtcct tgtgatgact cattcgtaat
    17401 aattgaggct tgattttctt cacttcccgt tatcgtttgt ccactgccag aatctgaagg
    17461 acttgttcct gtcgactcgt ttttttgaga agagacagtt gttttcttga cttgttcatt
    17521 tgtctttttc cattccgcta aacgatcggc gaccactttt ttgtctactt tttcagaata
    17581 agcggtgatc acttttccat ttttcaaacg ggctactttg tcgccatcat gagtcaaaag
    17641 caaaacggtc aaatgcttgt ctttaaattg atattgtctg agagaaatcg cactaagtac
    17701 gtcatcgata ttaataaatt gattttcttc ttttcctttt ggcaaataaa tgtccaattc
    17761 gcctttttcg taattggcag ttattttata ctccgcccac caataagttg agaattcttt
    17821 tttgaaatta tctactgatt ctttgcttgc tacgattttt tctgtttctt tctgagcaag
    17881 atctgctgtc atttttcctg aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa
    17941 taaccaaaag agaagaaacg gtcttttttt cctcatgctt tcctccactc ctatttaat
//
LOCUS       DACCZY010000055        17982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCZY010000055 DACCZY010000000
VERSION     DACCZY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.055"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(66..1544)
                     /locus_tag="IVZ67_001564"
     CDS             complement(66..1544)
                     /locus_tag="IVZ67_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8165644.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1842..2777)
                     /gene="murB"
                     /locus_tag="IVZ67_001565"
     CDS             complement(1842..2777)
                     /gene="murB"
                     /locus_tag="IVZ67_001565"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8165645.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2850..3770)
                     /locus_tag="IVZ67_001566"
     CDS             complement(2850..3770)
                     /locus_tag="IVZ67_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8165646.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3883..4635
                     /gene="xth"
                     /locus_tag="IVZ67_001567"
     CDS             3883..4635
                     /gene="xth"
                     /locus_tag="IVZ67_001567"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8165647.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5499..6032
                     /locus_tag="IVZ67_001568"
     CDS             5499..6032
                     /locus_tag="IVZ67_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8165648.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6522..7046)
                     /locus_tag="IVZ67_001569"
     CDS             complement(6522..7046)
                     /locus_tag="IVZ67_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165649.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7039..7512)
                     /gene="tsaE"
                     /locus_tag="IVZ67_001570"
     CDS             complement(7039..7512)
                     /gene="tsaE"
                     /locus_tag="IVZ67_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8165650.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7635..8615)
                     /gene="pta"
                     /locus_tag="IVZ67_001571"
     CDS             complement(7635..8615)
                     /gene="pta"
                     /locus_tag="IVZ67_001571"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8165651.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8768..9448)
                     /locus_tag="IVZ67_001572"
     CDS             complement(8768..9448)
                     /locus_tag="IVZ67_001572"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8165652.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9574..10443
                     /locus_tag="IVZ67_001573"
     CDS             9574..10443
                     /locus_tag="IVZ67_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8165653.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10469..10984
                     /locus_tag="IVZ67_001574"
     CDS             10469..10984
                     /locus_tag="IVZ67_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165654.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11031..11594)
                     /locus_tag="IVZ67_001575"
     CDS             complement(11031..11594)
                     /locus_tag="IVZ67_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165655.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11649..11828)
                     /locus_tag="IVZ67_001576"
     CDS             complement(11649..11828)
                     /locus_tag="IVZ67_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165656.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11905..12414)
                     /locus_tag="IVZ67_001577"
     CDS             complement(11905..12414)
                     /locus_tag="IVZ67_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165657.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12510..12890)
                     /locus_tag="IVZ67_001578"
     CDS             complement(12510..12890)
                     /locus_tag="IVZ67_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8165658.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13220..13969
                     /locus_tag="IVZ67_001579"
     CDS             13220..13969
                     /locus_tag="IVZ67_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8165659.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14153..15430)
                     /locus_tag="IVZ67_001580"
     CDS             complement(14153..15430)
                     /locus_tag="IVZ67_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165660.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15509..16153)
                     /gene="pcp"
                     /locus_tag="IVZ67_001581"
     CDS             complement(15509..16153)
                     /gene="pcp"
                     /locus_tag="IVZ67_001581"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8165661.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16352..17296)
                     /locus_tag="IVZ67_001582"
     CDS             complement(16352..17296)
                     /locus_tag="IVZ67_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8165662.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17298..17954)
                     /locus_tag="IVZ67_001583"
     CDS             complement(17298..17954)
                     /locus_tag="IVZ67_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8165663.1"
                     /translation="MIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFV
                     NNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNV
                     SFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGG
                     AGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ
                     "
BASE COUNT         5226 a         3678 c         3117 g         5961 t
ORIGIN      
        1 cttttctttg aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat
       61 aaatactagc tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca
      121 aaatattccc agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat
      181 ttttcaagta aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc
      241 acaatattgt aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat
      301 ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata
      361 ccgaccaata aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc
      421 aagaaatagc gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac
      481 gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat
      541 atccaatttt ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt
      601 ctttcttctt cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt
      661 ttttgactaa tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct
      721 aattcaaatt cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc
      781 tgctctttag ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct
      841 tctgagctct taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag
      901 gccctcaaca aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt
      961 tttaaattga acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt
     1021 tctctaaatg gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc
     1081 cgtatgttta tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca
     1141 agaaaagaga ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc
     1201 tctgcaaatt tttcgatcgt tacttttgaa tccacgatct ccgttaacag tcgagcaatt
     1261 tttgatcgtt gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt cagacgttcg
     1321 acatggatct ctgcttcttc tgaaatttgg atctctggct ttgttttgaa ttcagtaaat
     1381 tctttattta attctatgat ataatttctt gttttaaatt tagacaaacc taatgtttca
     1441 tataacttag gcagcactac ataattcttt ttctcaaatt ctaaatatct agctagcagc
     1501 agtttttctc tatcttcctc ttctataagg taatccaact tcatatccta cacctcaaaa
     1561 aatgcctcat accaattact tgatatgcag atgaaagttc atcttttcac ctatactgat
     1621 tttatgatgg attatgcttg ttttttcatc agttattctc attttttatc ttttttttga
     1681 gaatattgca aaaaatggaa aaatttatga ggaaatattc tatatttttt gttttattgt
     1741 gttaaaatgt acaagatttg cgccatttgc gtaaacaacc acaaaaaaga gcaggacggc
     1801 caccgtcctg ctcttttact tattttaagt tccattactt attaattggc taaacttctg
     1861 cttgtttctg atattgcttg tttttctcca atgatccgca cttctgtttc taaagacaca
     1921 tcaaattttt ctttgattac ttcttggatg tgcgcaatca attcaatata atctgtcgct
     1981 gtcgcatgat cgatattgac gatgaatcca gcatgttttt cagagacttg tgcaccgccc
     2041 catttcaggc cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg tcctactggg
     2101 cgcttaaaga cacttccgca tgaagggtat tctaatggct gttttgattg acgcaattcg
     2161 gtcaactcat ccattcttgc tttgattgct tcataatccc ctgtttgcaa actgaatcga
     2221 gcttccaata cgatagcacg taattcttgg actttactgt gacgataaga aaataccatt
     2281 tcgtcttttg tcaaagtttt caatgttcca tcttcaagca aaagatccac ttcagcaaaa
     2341 acatctttga tctctccgcc atacgcacct gcattcatat aaaccgctcc accgacactt
     2401 cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc
     2461 gtatcaatca gtgaggcacc tgcttccgca atgaccatcg ttcctttgac atgaatctgt
     2521 ttcatttcag tcagcatgat gactacaccg cgaatcccgc catcttgaac gatcagattg
     2581 cttgcatttc ctaataccat ccaaggtata tcatggattc ggcaataatc gatgatttgc
     2641 tttacttctt cttgtttttt cgggaatgca aggacatcag ctggtccacc tgttttcgta
     2701 aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa gctttcaact
     2761 atttcttctt tattcactga ggattttcct ttcttttctc aaacgtcctc attattctat
     2821 cacgcttaat acaatcttgc cagtcttgtt caaagttgat gacggtagtg atctttaagc
     2881 acttcctcac ataattcatg acttcttaca acttcttttt gtttaagcaa ttctgtttcc
     2941 gatttaccta aacggatctg agaaaagaag gcctctacca ttggctcaaa cccgcgtttg
     3001 actagcgtgt ttgtccagtc gccaaattct ttcacttttt ttccatcagc atcttgaatg
     3061 acaagctggg tcagatcgtt tacttcataa gtaccatttt tacttgttac ttgatatttt
     3121 tctatattag cgccacttgc taaatccatc gttaaaatag ccgtttgatt ttgtgtttct
     3181 aattgtaaaa tggcatattc caaaaattcc tttgtttctc ttatctggta ttttgttctc
     3241 aaaatgggtt catctaataa atacacagct gtatcaataa gatgtaaaaa caaatcataa
     3301 aggacgaact ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg gatcaaccgc
     3361 ttatctggga tacgtttcaa ttcttctacc ataggagcaa agcgccggtt gaaaccgatc
     3421 atcaaaatta tttggttctt atcagctaat tcttgcaatt cacgaacttc atttagatgg
     3481 acactcagtg gtttgtcaat ataaacatgg ataccatttt ctagacattt tttggctaat
     3541 tcataatgta cttttgttgc tgcgtggatc atacatgctt cgattttttc ttctaaaagc
     3601 tcatcgatcg tttcatatag atgttggaac ccgtactctt tttgaattct ttcttttgtt
     3661 tctttatttc ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc ataagttggt
     3721 aaatatgctt tttctgcgat attccctaca ccgattacac caatcttcat ctactttcct
     3781 cctggatcat ttagttactt tcatcttaca ccattttttt ctaaaatgag gtacaatagt
     3841 atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt tattgaaatt tatctcttgg
     3901 aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt tcatgagtta
     3961 gacgccgatt ttttctgtct gcaggaaacg aaactccaag caggacaaat tgatttagat
     4021 cttccaggtt attatcaata ttggaattac gcggaacgaa aaggctattc cggcacagcg
     4081 atttttgcta aaaaaccagc attgaatgcc acttacggaa tgggtatcga tatccatgat
     4141 acagaaggcc gtctgattac tttggaatat tccgatttct ttcttgtaac ctgttacacg
     4201 ccaaattctc aaagcgaatt aaaacgttta gattaccgat tagaatggga agaagccttt
     4261 tataattatt tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat
     4321 gtcgctcatc aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc
     4381 actccagaag aaagagcggc tctttctcga ctgttggata acgggtttat cgatacattc
     4441 cgttattttt atccaaccca ggaaggtgtc tactcttggt ggaactaccg attcaattca
     4501 aggaaaaaca atgctggttg gcggatcgat tactttttga cgagcaaaga tttagagcca
     4561 ggactagctg atgcaaagat ccatacggac attatgggca gtgatcattg tccagtagaa
     4621 ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt
     4681 ttgtgaaaat ggctttccac attttgcata aatttggcat aataccgaga aactggcttt
     4741 tgaacactgt ttatcgagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc
     4801 caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc agatacaagc
     4861 ttttgaacac cgtttattaa gaggttgtga aaaagctgca ctgcatcaag taataagaac
     4921 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagatgca
     4981 agcttttgaa caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag
     5041 aacagccaaa caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat
     5101 gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt
     5161 agagaaacaa atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg
     5221 gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc
     5281 gtaagaaatt tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga
     5341 gaagtcatga tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc
     5401 tcaacaaata ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa
     5461 agtatgttac agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt
     5521 ttgaaactgc caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa
     5581 atagccagat cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc
     5641 gaaatgtcca aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag
     5701 aagtctggca aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg
     5761 ctgcttttga tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta
     5821 attatctttc tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc
     5881 atcgattgaa cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat
     5941 tggaagatag tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag
     6001 atccattgaa aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg
     6061 tcgtgagcct gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat
     6121 gtttattaaa tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag
     6181 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
     6241 catatttttc atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc
     6301 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg caaaatagtt
     6361 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat
     6421 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
     6481 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
     6541 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
     6601 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
     6661 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
     6721 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
     6781 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
     6841 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
     6901 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
     6961 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
     7021 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
     7081 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
     7141 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
     7201 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
     7261 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
     7321 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
     7381 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     7441 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     7501 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     7561 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     7621 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     7681 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     7741 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     7801 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     7861 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     7921 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     7981 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     8041 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     8101 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     8161 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     8221 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     8281 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     8341 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     8401 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     8461 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     8521 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     8581 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     8641 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     8701 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     8761 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     8821 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     8881 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     8941 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     9001 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     9061 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     9121 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     9181 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     9241 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     9301 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     9361 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     9421 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     9481 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     9541 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     9601 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     9661 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     9721 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     9781 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     9841 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     9901 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     9961 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
    10021 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
    10081 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
    10141 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
    10201 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
    10261 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
    10321 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
    10381 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
    10441 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
    10501 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
    10561 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
    10621 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
    10681 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
    10741 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
    10801 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
    10861 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
    10921 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
    10981 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
    11041 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
    11101 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
    11161 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
    11221 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
    11281 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
    11341 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
    11401 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
    11461 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
    11521 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
    11581 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
    11641 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
    11701 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
    11761 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
    11821 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
    11881 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
    11941 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
    12001 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
    12061 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
    12121 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
    12181 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
    12241 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
    12301 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
    12361 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
    12421 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
    12481 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
    12541 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
    12601 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
    12661 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
    12721 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
    12781 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
    12841 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
    12901 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
    12961 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
    13021 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
    13081 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
    13141 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
    13201 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
    13261 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
    13321 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
    13381 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
    13441 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
    13501 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
    13561 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
    13621 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
    13681 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
    13741 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
    13801 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
    13861 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
    13921 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc
    13981 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaat
    14041 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
    14101 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctatt ttttatagta
    14161 attccttcag atactttctt gcctcatcat tgaaacgcag tgattcttct aacttctgat
    14221 aatattccaa cgcttgtttc tgagcttcag taaaacgata atgttttcct ttccatttgt
    14281 cgattgtctc ttgcacattt ccagattgga tatctttcac tactagttgc catttttctt
    14341 ttgagactgt acggtctttc ttatcagcag attgtatagc ttctcgttct ttacgcaata
    14401 cttttaaaaa accatttctg actatccgag gaatgctgtc gaagaaccct tctgtaaaac
    14461 catagcgaca atattgcttc caaataaata gatagatttt tggttggatg aatcgttggt
    14521 ttttcgcaaa atattccatc tgagcagtga cttctggtgt tcgttgacgt tttgtctttc
    14581 tcaacacgga aataaatgtt tcaaaataag cattaggatg tactggcact tcttcatata
    14641 cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat
    14701 cctttactaa ttcttctgca aagtcgtcaa atagttcttt ttttatctgt cgcttttttg
    14761 actgcccatt gatgtgttca cttattttct tgatttcatt ttccaatgct tgataatccg
    14821 ctatgtaaat cggcatttct acttggcgaa attctgcgag ccgctgagct aattttatat
    14881 ccggtaaata tggctgtttc atccgacgat tttggtacaa gacaaaacag tatttttgcc
    14941 atagtccttc aggaaaatcg atattcttct gcttaagcca taataattcg tctaaggcca
    15001 gatcaacttt tgtcattccc tgatggatcg aagctgccac tgtcggagag acatgcaggt
    15061 gatcggcaaa gtggttgatg cctagtttta ggatctttcc ggtttttggc gatttaacga
    15121 tgtattgcac ttttaattct ctaccacact cacaatacaa tttttgctgg cttcgttttg
    15181 ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca atctgttgca gctaaataat
    15241 gttcttccat aaaacgagag ctgattagat atcggcggtg cttatcgact agttcccgct
    15301 gttttttcgt taattttgca tagatctccc aacgttgact gcttgtgtat tgtttagaaa
    15361 ttttgatggg atagcttttg ctgatttctt ttggaaacca ttcttcaaag acggattgcc
    15421 attcttccac ggctgtttct ttcctttcta attacaaaaa gctggctact aacataacgt
    15481 tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt tccggcattt ccggaaatat
    15541 cttctttatt ttctactgct gcttcaactg cataagtcaa agaagaagca atgtcttcta
    15601 aagacataga aggcgtaccc actggacgat cgatcacttg atcgggtgag aatggcacgt
    15661 ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat caaatgaagc aaacgataca
    15721 tgatatcatt gcagacaaat gttcctgctg tataagagat gtacgctggt agaccatgat
    15781 cttgtacatt ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg gctggaccgt
    15841 tttcttctaa agccgtatcg aatggctgat ttccttcatt atcagggatt cttgcttctg
    15901 ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct tgtccgacac
    15961 agatcacgac atctggttgg tgctgttgga tggcttcttt gactacttga ctgctttttt
    16021 cgaatacggt cggtacttcc actttgatga tttcagcacc gctgatttca tctggcattt
    16081 tttttactgc ttcataggct ggattcactt tgtccccacc aaatggatca aaacctgtta
    16141 ctagtacttt catctgttgt tcctccgtcc gattgattta acatatggtt atcttttatc
    16201 ataccatttt ccttgaacag cgtaaataaa ggaaacaata gtttaaataa cataacagat
    16261 caaacttaat atattcttat aaaaaaatag tgttccacta caatgttaga agaggttgcg
    16321 acaattattc tgtcacaacc cctctcttct attatttaaa tagaatcata taagcaattt
    16381 ggaaaactag cataaataac gcaataaaca actggttttt gatcactccg tatttgtctt
    16441 tcatctccag catcgctaca ggcacgatat tgaagttagc agccatcggt gtcagcaggg
    16501 tcccacaaaa accgcaagtc aatgcaacca ttcctattaa cgctggattc gctccatgac
    16561 taaatacgaa tggtgcaccg atcccaaccg tcatcacggt gatggccgca aatgcattcc
    16621 ccatgattac cgtaaatatc accatgccaa gagcgtaaat gatgatgccg atgttcacat
    16681 ttccttctgg aacgatcgta cctactgcat tggaaatcac tgtcccaact ccagcgcttg
    16741 taaataccgc acctagagaa gctaaaagca ttggcaacat gcttaacggc cctactgtgc
    16801 ccagcatatc tgctgcatca tctaaaaatg tacttggtcg attatctcga gaaaagaaca
    16861 tcaagattag catggctgca aaaacaccga cgcccacgcc gactaaagca cctaaattgg
    16921 taaatagagc aaacaagatc gcaaagacac cgatactcaa agctggaata aaaattttca
    16981 ttcctagttt gtctgacatt ttttccatat attcttttga tggaagccgg ctttctcctt
    17041 tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga
    17101 ttgccggaag ccatcttcct cctgcaataa ctacccctaa gaccgtccag aaaaaggctg
    17161 acccataacg atgaggattg gttttatctg ttaaatttct tactccagtg taaatcaata
    17221 cgagtcccat cacgatataa aggatctcca gtaatttatc gcctaataaa atatcaggat
    17281 ttgtaaaaaa gtccatctta ttgccccttt cctggataat atttcttcgc taatttcttt
    17341 tctttaagat agaagtagac acttgccaca actaaagccg tgatagccac tggaacttcc
    17401 actaaagcaa gctgtcccaa tgttacgtca tatcctagat ctttcagtgt tgattggact
    17461 agcaacgccc ctgctcctcc gatgaatagt acttggccaa agaaccagca gacattttcc
    17521 atagctgaag ccataccttt tagttcttct tcatgcttag gatggacttc taaccctttt
    17581 gattcgatgg tccccaatgc catcggtaaa atgattggac gaacaaatcc agcaactcca
    17641 ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga tcccataaat atctacaatc
    17701 ataccagcag atactttctt gaatcgcttg atcaaggaag ccgctgattc tttcagacca
    17761 tttctttcaa gcgttcctgt tgccaacata atgatgataa aaatcgccat cccgcgattg
    17821 tttacaaaac ttgtacctaa aagttctaac attccttgaa tgccaagacc gccagtcaaa
    17881 gctgtaacaa ctaaagcaac cattacgatc aatatcgagt ctaatttaaa tgcaaacccg
    17941 agaataacaa tcaagacgcc taaaaggcta agataatttt cc
//
LOCUS       DACCZY010000056        17248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCZY010000056 DACCZY010000000
VERSION     DACCZY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.056"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(8..553)
                     /locus_tag="IVZ67_001584"
     CDS             complement(8..553)
                     /locus_tag="IVZ67_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8165664.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(565..1257)
                     /locus_tag="IVZ67_001585"
     CDS             complement(565..1257)
                     /locus_tag="IVZ67_001585"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8165665.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1276..1560)
                     /locus_tag="IVZ67_001586"
     CDS             complement(1276..1560)
                     /locus_tag="IVZ67_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165666.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1573..2136)
                     /locus_tag="IVZ67_001587"
     CDS             complement(1573..2136)
                     /locus_tag="IVZ67_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8165667.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2364..3026
                     /locus_tag="IVZ67_001588"
     CDS             2364..3026
                     /locus_tag="IVZ67_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8165668.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3150..4346
                     /locus_tag="IVZ67_001589"
     CDS             3150..4346
                     /locus_tag="IVZ67_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8165669.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4447..5823)
                     /locus_tag="IVZ67_001590"
     CDS             complement(4447..5823)
                     /locus_tag="IVZ67_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165670.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5860..6726)
                     /locus_tag="IVZ67_001591"
     CDS             complement(5860..6726)
                     /locus_tag="IVZ67_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8165671.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6815..7840)
                     /locus_tag="IVZ67_001592"
     CDS             complement(6815..7840)
                     /locus_tag="IVZ67_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8165672.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7858..8532)
                     /locus_tag="IVZ67_001593"
     CDS             complement(7858..8532)
                     /locus_tag="IVZ67_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8165673.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8716..9609)
                     /locus_tag="IVZ67_001594"
     CDS             complement(8716..9609)
                     /locus_tag="IVZ67_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8165674.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9683..11380)
                     /locus_tag="IVZ67_001595"
     CDS             complement(9683..11380)
                     /locus_tag="IVZ67_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8165675.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11373..12686)
                     /locus_tag="IVZ67_001596"
     CDS             complement(11373..12686)
                     /locus_tag="IVZ67_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8165676.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12686..16723)
                     /locus_tag="IVZ67_001597"
     CDS             complement(12686..16723)
                     /locus_tag="IVZ67_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8165677.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16736..17194)
                     /locus_tag="IVZ67_001598"
     CDS             complement(16736..17194)
                     /locus_tag="IVZ67_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8165678.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4830 a         3885 c         3008 g         5525 t
ORIGIN      
        1 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
       61 gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat
      121 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
      181 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
      241 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
      301 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
      361 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
      421 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
      481 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
      541 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
      601 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
      661 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
      721 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
      781 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
      841 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
      901 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
      961 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     1021 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     1081 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     1141 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     1201 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     1261 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     1321 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     1381 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     1441 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     1501 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     1561 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     1621 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     1681 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     1741 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     1801 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     1861 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     1921 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     1981 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     2041 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     2101 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     2161 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     2221 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     2281 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     2341 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     2401 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     2461 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     2521 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     2581 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     2641 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     2701 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     2761 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     2821 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     2881 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     2941 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     3001 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     3061 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     3121 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     3181 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     3241 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     3301 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     3361 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     3421 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     3481 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     3541 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     3601 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     3661 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     3721 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     3781 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     3841 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     3901 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     3961 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     4021 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     4081 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     4141 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     4201 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     4261 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     4321 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     4381 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     4441 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     4501 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     4561 tacaatacaa gtcaaaatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     4621 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     4681 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     4741 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     4801 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     4861 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     4921 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     4981 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     5041 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     5101 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     5161 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     5221 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     5281 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     5341 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     5401 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     5461 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     5521 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     5581 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     5641 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     5701 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     5761 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     5821 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     5881 taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt
     5941 tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat
     6001 ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat
     6061 gtttcctaag ccccataata atccacagat catgtttttc cacacaaact gatcgacttt
     6121 aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat
     6181 aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat
     6241 cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc
     6301 tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa
     6361 agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc
     6421 cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc
     6481 catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa
     6541 aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa
     6601 agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat
     6661 tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat
     6721 ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac
     6781 aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta
     6841 agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat
     6901 ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg
     6961 ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt
     7021 ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt
     7081 ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt
     7141 ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga
     7201 tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa
     7261 tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg
     7321 cataatacaa aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg
     7381 tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa
     7441 gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac
     7501 tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta
     7561 ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt
     7621 gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa
     7681 aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga
     7741 tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga
     7801 tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca
     7861 gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc
     7921 gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc
     7981 gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat
     8041 caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc
     8101 catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa
     8161 ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat
     8221 ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat
     8281 attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg
     8341 acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc
     8401 ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg
     8461 ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat
     8521 gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc
     8581 cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg
     8641 gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc
     8701 ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg
     8761 ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa
     8821 tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg
     8881 aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat
     8941 aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac
     9001 aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg
     9061 gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg
     9121 ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac
     9181 tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt
     9241 gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt
     9301 ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac
     9361 tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt
     9421 ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata
     9481 attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat
     9541 cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt
     9601 ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat
     9661 tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc
     9721 tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac
     9781 cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta
     9841 ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg
     9901 ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg
     9961 ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg
    10021 cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg
    10081 actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg
    10141 catctttaag caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg
    10201 tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt
    10261 caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat
    10321 tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt
    10381 tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta
    10441 ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat
    10501 cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc
    10561 ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag
    10621 ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa
    10681 taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga
    10741 tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat
    10801 cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc attggagacg
    10861 cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag
    10921 aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg
    10981 ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt
    11041 tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag
    11101 gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc
    11161 ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg
    11221 gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga
    11281 cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta
    11341 acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt
    11401 tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt
    11461 gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca
    11521 ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt
    11581 gcggattcat aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg
    11641 ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc
    11701 tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa
    11761 taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa
    11821 atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga
    11881 tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc tatcatctga
    11941 tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt
    12001 ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc
    12061 tcttgcagtt cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg
    12121 atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa
    12181 atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg
    12241 tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc
    12301 ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg
    12361 aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg
    12421 taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc
    12481 cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa
    12541 tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct
    12601 tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga
    12661 agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa
    12721 acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata
    12781 atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct
    12841 gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg
    12901 tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc
    12961 ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa
    13021 agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc
    13081 cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc
    13141 cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt
    13201 caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt
    13261 catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag
    13321 cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa
    13381 actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat
    13441 attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc
    13501 ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta
    13561 acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat
    13621 cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc
    13681 gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg
    13741 tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag
    13801 cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg
    13861 ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat
    13921 aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat
    13981 atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt
    14041 tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt
    14101 cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga
    14161 tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat
    14221 actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat
    14281 aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat
    14341 ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga
    14401 attgatacgt ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa
    14461 atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg
    14521 ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat
    14581 tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc
    14641 tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc
    14701 gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca
    14761 ctgtgcaaaa atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga
    14821 ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga
    14881 catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc
    14941 atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa
    15001 tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg
    15061 tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt
    15121 cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa
    15181 tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat
    15241 cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc
    15301 aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat
    15361 tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt
    15421 ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg
    15481 cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat
    15541 ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc
    15601 aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg
    15661 tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt
    15721 tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg
    15781 tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa
    15841 agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca
    15901 ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta
    15961 ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt aattcactca
    16021 tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc
    16081 ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag
    16141 atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat
    16201 ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa
    16261 cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc
    16321 agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg
    16381 agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat
    16441 agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat
    16501 agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga
    16561 ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct
    16621 tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat
    16681 tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc
    16741 cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt
    16801 ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt
    16861 ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc
    16921 cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt
    16981 caaaagccag atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg
    17041 catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat
    17101 ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta
    17161 ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt
    17221 tgtcttcttt cacaacaatc aacaaaac
//
LOCUS       DACCZY010000057        16897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCZY010000057 DACCZY010000000
VERSION     DACCZY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.057"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            86..1786
                     /locus_tag="IVZ67_001599"
     CDS             86..1786
                     /locus_tag="IVZ67_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8165679.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1756..1974
                     /locus_tag="IVZ67_001600"
     CDS             1756..1974
                     /locus_tag="IVZ67_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165680.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1994..2884
                     /locus_tag="IVZ67_001601"
     CDS             1994..2884
                     /locus_tag="IVZ67_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8165681.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2902..3186
                     /locus_tag="IVZ67_001602"
     CDS             2902..3186
                     /locus_tag="IVZ67_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165682.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3673..4287
                     /gene="gmk"
                     /locus_tag="IVZ67_001603"
     CDS             3673..4287
                     /gene="gmk"
                     /locus_tag="IVZ67_001603"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8165683.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4292..4597
                     /locus_tag="IVZ67_001604"
     CDS             4292..4597
                     /locus_tag="IVZ67_001604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8165684.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4850..7258
                     /gene="priA"
                     /locus_tag="IVZ67_001605"
     CDS             4850..7258
                     /gene="priA"
                     /locus_tag="IVZ67_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8165685.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7273..7764
                     /gene="def"
                     /locus_tag="IVZ67_001606"
     CDS             7273..7764
                     /gene="def"
                     /locus_tag="IVZ67_001606"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8165686.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7757..8695
                     /locus_tag="IVZ67_001607"
     CDS             7757..8695
                     /locus_tag="IVZ67_001607"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8165687.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8695..10053
                     /gene="rsmB"
                     /locus_tag="IVZ67_001608"
     CDS             8695..10053
                     /gene="rsmB"
                     /locus_tag="IVZ67_001608"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8165688.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10066..10806
                     /locus_tag="IVZ67_001609"
     CDS             10066..10806
                     /locus_tag="IVZ67_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8165689.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10803..12872
                     /gene="pknB"
                     /locus_tag="IVZ67_001610"
     CDS             10803..12872
                     /gene="pknB"
                     /locus_tag="IVZ67_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8165690.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13037..13936
                     /gene="rsgA"
                     /locus_tag="IVZ67_001611"
     CDS             13037..13936
                     /gene="rsgA"
                     /locus_tag="IVZ67_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8165691.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13949..14599
                     /locus_tag="IVZ67_001612"
     CDS             13949..14599
                     /locus_tag="IVZ67_001612"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8165692.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14600..15238
                     /locus_tag="IVZ67_001613"
     CDS             14600..15238
                     /locus_tag="IVZ67_001613"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8165693.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15393..16247
                     /gene="mreC"
                     /locus_tag="IVZ67_001614"
     CDS             15393..16247
                     /gene="mreC"
                     /locus_tag="IVZ67_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8165694.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16251..16760
                     /gene="mreD"
                     /locus_tag="IVZ67_001615"
     CDS             16251..16760
                     /gene="mreD"
                     /locus_tag="IVZ67_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8165695.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         5663 a         3044 c         3698 g         4492 t
ORIGIN      
        1 tttcttatgg aatctacaaa gtcgaatacg agactcatga cctttttatg acgtactggt
       61 atacttgaag aaaaaggaga gcgcgatgga gataaaaaaa gtttactttt cacagaaaat
      121 gtcgctgtat tcgcctatcc agcaagcaaa acaacccctt ccattttttc aaagtcatca
      181 agaaagtaga gacgcttttt ggcgttctgc aaaaaagcaa tgtgctttta aagcagtatc
      241 gaaaaaatat accgtctatt ttactattgc taaaagtaga ggagatgtta tttttaataa
      301 tctgttgatt gaaggtgaaa cttttgaatg gaaaaaattt tttcggtata tggaagcttg
      361 cgctcgtttt tttattaaag aaaactgttg ttttttattt caatctcctc tatcagaaga
      421 atggttgcgt atttttcata aaaacggcta tcaaggaacc acacaattaa ataaaaagct
      481 ggtctatcat acagcacttg ttttaggtgg aggaggagcg cacggtgctt atcagattgg
      541 tgtgtggcag gcgctgaagg aacatgatat caactttgag attatcacag gtacctcggt
      601 aggtgcgttg aacggcgcat tgattttaca aggagatatg aaaaaagctg tgaatttatg
      661 gaaaaagctg tcgacaagac aagtgcttgc gttaccagaa atggctgcag ttgaagacct
      721 tcgtgaacgc ttctatcgag aaagacgcca aatgacgaaa acagcactaa ttgaaggcgg
      781 ggtttcttct gctccattgg aaaagatggt caaagacaat ttggatttgt ccttactcaa
      841 acataatccc aaaatacgtt tatatacggt gtctactaaa ctgccagaac tagcggaagt
      901 agtgactgat atccaacaga caaaaacaga agaaatacca gattggatac tcgcctctgc
      961 ttcattttat cccgctatgg cttatcgaaa gatcggaaaa aataaatatg cagacggtgg
     1021 gtatcgcaac aaaataccta ttgatattgc gattaacaaa ggagcaacag aggcatttgt
     1081 cgtagatgtg caagggccag gcccagcgaa aaaaattcgt atgccagaca tttttatcca
     1141 ttggaaatgt caaacattgt ggacactagg cagctttctg ctttttgata gccaaaggaa
     1201 ccaattaaat ttacaattag gctatctgga aatgaaaaaa cgattaggct attactatgg
     1261 gaactggtat acatttcatt ctgttaaacc tgcaagaaca tactggcgag gatttttgtc
     1321 ttacttaaca aaagaaattc agttagaatt atcttttttc aaaagcataa aattttggca
     1381 aaaactgcgt aatttatata agaacagggt cgtaccagag acatgcggat tggcgatgct
     1441 agaactttta gcaaaaaaac attttttact gcccaatgaa atctatcaag tagacatgat
     1501 gattcaaatg atctgccaac aagatataaa aagtatgccg gatgatttat tggcacaaat
     1561 tggtcaatta agtactgaag aatggcgaag gtatcgaaaa taccagcaaa aaatacaaaa
     1621 tgagcgcacg aaaactgttt attttgatta tctgctacaa ggaaaacgca aggatcgttt
     1681 gcagcatgaa ttgcttaagc aaccagttga cacattactg atcctatatt tatattatct
     1741 caaggaggaa caaccatggc acaagaattt tcatatgaaa tcgtagaaga aatcgctata
     1801 ttgtcagaaa acaacaaggg gtggcgtaaa gaactgaacc tagtcagctg gaatggtcgc
     1861 ccgccaaaat tcgatcttcg agattgggcg ccagaccatg aaaaaatggg gaaaggcctg
     1921 acattgacga acgaagagtt tgaacaactg caaaaagcaa tagaaaatat gtaatgagga
     1981 aggaccctgt cgaatgaaaa gtatgactgg ttttggcaaa gccatccgcg aaacaaaagc
     2041 gtatcagtta gatattgaaa taaaaagtgt caaccagcgt tttctagatg ttcaaatacg
     2101 aagtcctaaa atcctgaact ttgttgaaaa cgattttcgt cagcttatta aacagcagtt
     2161 aagccgcggt cgaatcgaag tatttgtgaa tctagcttat attggcaaaa acgaaaaaca
     2221 gatcgtcatc aactgggaat tgattgattc acttatcact caattgacag acggaattca
     2281 acaacgatac ggtagtcaaa atcaagtgtc aattagtcgg atggcggaac gcttcgtgct
     2341 ggacgaaaac ttcgtaacgg tagaagaaaa accggaagag agcatggaag aattggcaca
     2401 gttagctcta gatacattga aaagcgcact tgattctatt gaggcgagtc gagaaaaaga
     2461 aggtcaagct ttagctttag tgttgaaaaa gaatactgaa gaattcaaag aagtactaaa
     2521 aaaactcgat agttttgtag aaatctatga aaaggaattt caaataaggt atcaaaaaaa
     2581 gctggaggaa tttcttggag cgaaagtcga tgagcaacgg ttgttgacgg agctagccat
     2641 cttgttggaa cgcggagata ttcacgagga actggatcgt ctggcgatcc atgtacaaaa
     2701 actagatgaa cttatcgaag cacaatgtcc tgtaggtcgg gaattagatt tcctgatcca
     2761 agaaatgaat cgggaaatca acacgatcgg ttccaaatca aatgcaatcg aaattaaaaa
     2821 tcaagtcgtc cagtctaaga cgatcttaga aaaaatcaga gaacaagtac aaaatattga
     2881 atgacgaaag aggtagagat aatgaaatat tatgacgtaa cgttccacga attaagtgga
     2941 aaaactgtgg ttaaacggga tatcccttct gaaaaaaacg gctttgacgt atggaaagat
     3001 gcctgtgcag actataatga gaacgaactg tttattttga tcaacgacgg tgcttacgtg
     3061 acgatgaatc ggaaatttat tgttcgaatt gacacagaag aggtagaaga tccgacagaa
     3121 aaagcacgtt ctcgaaaaga tgagatcatg ggtgtagtga atactttgtc caatatgggc
     3181 ttttagcaag gatgctgaac aaagaaatac aataaataaa cagaattgga tttcttcgct
     3241 tagcttcttt gctctaggaa tacatttaaa ggttgtgaca aaattcgtgt cacaatcttt
     3301 ttttattttg aataagtgat gttcaaaaac cattttctcg gtattaagcc aaatttatgc
     3361 aaaaatgtga gaaactattt tcacaaaatt tttcttcata ctcgaaacta atcggcttct
     3421 tcacgacctc ttatacacgg cgttcaaaag cttgcatctg cgataataac cccaaccaaa
     3481 cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat tacttgatgc aggacagctt
     3541 ttttacgacc tcttggtaca gggtagcttt tttaccaatt tctagctata attaaagata
     3601 accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata agtacaaaaa gactagttga
     3661 aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt tgtctggtcc atccggtgta
     3721 ggaaaaggca cagtacgaaa agcaattttt gagagtgagg ataatgattt tcagtattcg
     3781 atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg aaggcgttga ttatttcttc
     3841 cgaagcaaag aagagtttga atcaatgatc gaagctggag agatgctgga atacgcagaa
     3901 tatgtcggga attattatgg aactcctttg tcatatgtca acaagacatt ggatgaagga
     3961 aaagatgttt tcttggagat tgaagtccaa ggagcaaaac aagtcaaaga aaaagtgccc
     4021 gatggtgtat tcatcttttt gactccgcca gatttagcgg aattaaaatc acggatcgta
     4081 ggccgaggaa cagatgcaga tgaagtaatc gaaaaacgaa tgaaagttgc aaaagaagaa
     4141 atcgaaatga tggctcttta cgactatgct gttgtaaatg atgaagtgcc gctagcagtt
     4201 gaacgaatca aaaacattat tgccagtgaa cattttcgag tagatcgcgt aatcggtaaa
     4261 tatattaaaa tgttaaagga gatgtagtct tatgatgtta aaaccttcaa ttgactcact
     4321 attagatcgt gtaaattcaa aatattctct agtgatttta gcaagtaaac gcgcgcatga
     4381 attggatgca agtgcacagc ctactctaga ttcttttgat tcagtaaaaa gcgtcggtca
     4441 agctttagaa gaaatcgatg caggaaatgt agtcaatgat ccacatcctg aattaaaacg
     4501 tgaacgtttg aagatggaag aagaggaacg tcaagcacaa aaagagcgtg aacaacacga
     4561 attggaatca cgtatccgtg aagagcaaaa aatgtaataa agaaaaatag agcgaacatc
     4621 taaaaagaaa agcaaaccca gctgtttcct tttcaaacat tcgccactta ttgtctggta
     4681 gaaaaggctg tgtctgaact ttttgacaca gttctttttt ttcttatctg ctgttaattt
     4741 tttgttagga tttggcagaa gataagacaa atccatcgat ctctagtaaa ataggcaagg
     4801 aactttttgt cgcttgataa gaaaagacaa aaaaaggagg aaacgaacca tgacattaac
     4861 agctgaagtc attgtggacg tacctactat gcagaccgat cagccattca cttatttggt
     4921 tccctctgaa atggaaacag ctttgcaagt cgggatgcga gtagaagtcc cttttggtaa
     4981 tggcaatcgt catgtccaag gatttgttat ggctatccaa aaatcagaag aatcagctaa
     5041 tccatctttg aaggcagtta ttcgtttgct agaccttgct ccggtggtca atgaagaatt
     5101 actttcatta gctgattata tgaaaaaaat tacttacgca ttcaaaatca cttgtctaca
     5161 aacgatgctt ccgagtgtga tgaaagcaga atatgataaa ttgatctatc ctttggcaga
     5221 cacaccagaa gttcaggtat tgtttgatca gcgagaagtg atttcctgga aggaagcaga
     5281 agaagaaggc agcctttctc aattgattcg ctggcgccaa gagcaattag tagatatcaa
     5341 atatgaagtc catacacgaa ataaagtaaa aacgattcgt ttggtccgtt ctttgttaac
     5401 tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa aatgccaaga aacaaaaaga
     5461 acttcttttg tgtttatctg aactgagcca agaagaacca attgcttatt tcaaaaataa
     5521 aggaatcagt actgctgtct tgaatcaagg aaaagaaaag ggctggctgg agtttataga
     5581 atcagaacgt tatcgcgacc cttataaaga ccgagtattt gaccaaacga cggcattgga
     5641 actgaatgca gaacaaaaaa atgctgtcga acaaatcatc acagcaggtc agcagcaaaa
     5701 agatcaggtc tttttgctgg agggaatcac tggcagcggg aaaacagaag tatatcttca
     5761 ggcaatcgct gatgttttgt cagaaaataa aacggctatt atgttagtgc ctgaaatcgc
     5821 attgactccg caaatggtcg agcgattcaa aggacgcttc ggagaatcag ttgcggtctt
     5881 acatagcgga ctttcccaag gtgaaaaata tgatgaatgg cgcaagattg agcgggaaga
     5941 agcgcaagta gtagtaggcg cacgatctgc tatttttgcc ccgctgaaaa atatcgggct
     6001 gatcattatc gatgaagaac atgaatcaag ttacaaacaa gatgagacac cgcgttatca
     6061 tgcgagagat ctagcaattt ggcgcagtaa atatcatcat tgcccaatag ttctaggaag
     6121 cgcgacgccc tctttagaat caagagcacg agctcaaaaa ggcgtttatc agttattgca
     6181 gttaaaccac cgagccaaag cagctgcaca gcttccagct attgagatcg tagatatgcg
     6241 tgaagagttt cagaatcatc ggacctctac tttttctgct aatttgcagg aaaaaataca
     6301 aaatcgatta gacaaaaaag aacaaactgt cctactgctc aatcgtcgag gatattcgtc
     6361 ttttgtgatg tgtagggact gtggatttgt tcttccatgt cctaattgcg atatctctct
     6421 tacactgcat atggatacac gttcaatgcg ctgccattat tgcggacatg aagaacctat
     6481 tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc tactatggaa caggtactca
     6541 aaaagtagaa gaggaactcc gagaacttta tccgcaagct aggattttgc gtatggatgt
     6601 agataccact cgaagaaaag gggcacacga acagatttta caaaaattcg gtgcaaaaga
     6661 agcagatata ttattaggga cacaaatgat tgctaaaggt ttggactttc cagaagtgac
     6721 attagttggg gtgttgaatg ctgatacctc cctgaattta ccggattttc gttctagtga
     6781 gcatacattc caattgctta cccaagtttc aggacgagca gggcgagcgg aaaaagcagg
     6841 cgaagtcgtg atccaaacat tcaatcctca acattacgcc atagaattgg ctaaaaaaca
     6901 aaattatgag cagttttatc agcaggaaat gcatgtacgg catcgtggag gctatcctcc
     6961 ttattacttt actgtgaaaa taaccgccag ccatcctgaa gaacaagtag cggcaaagaa
     7021 aatctttcag attgccaatc agctaaaaga agtgttaagc cctcaaagct tgctattagg
     7081 gccaacgccc agcatgattt tgagagtgaa aaaccgatat tattatcaac taatcatcaa
     7141 atataaacat gaaccgaatc ttcctcatgt tttggatgaa attttgaatg gaagccagaa
     7201 agaacagcga caaggtcttt ttgtggcaat tgacaacgaa ccattaaact ttatttaaga
     7261 aagggccaaa ttatgcgcta tcctatattg atccacccta atgataaatt aaaaagaact
     7321 gctcagccaa tcgatgtaat cacggacgag acaatcgctt tattagacaa tttatatgag
     7381 acaatgattg cgaatgacgg aatagggatc gctgctcctc aagtaggaca aaacaagaga
     7441 attgctgtga tcgaagtaga tgaaggcgag aaatttgaac tgattaaccc agaaatcatc
     7501 gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca gtattccgca tgtctatgga
     7561 acggtcaagc gagcagatga agtgaccgtg cgttattatg atcgagacgg agaagagata
     7621 gaagtgacgg cttttggcta tttagcccgt gccttccagc atgaaatcga tcacttagac
     7681 ggtatcttgt tcattgagaa aatgatacag cagatcccgg aagaagaact tgaagaatat
     7741 atggaggaac atcttgatga ctaagctagt atttatgggc acacctgctt tttctgtgcc
     7801 gattcttgaa gggctgatag aatcaggtta cgagatccag gcagtggtaa cacagcccga
     7861 ccgtccagtt gggcggaaac gagtaattac gccgacaccg gtaaaaaaag cggctctgaa
     7921 gcacggtatc cgtgtgctgc agccagagaa aatctccggt tcgccagaaa tggaagaaat
     7981 cattgaattg gtgcctgatg tgatcgtcac tgcggcattt ggccaattct tgcctgaaaa
     8041 gttattgcaa gtaccaaaat taggggcaat caatgtccat gcttctttac ttcctaaata
     8101 tcggggaggt gcgcctgttc attatgccat catgaatggg gagaaagaaa ctggtgtaac
     8161 gatcatggaa atgatcaaaa aaatggatgc aggcggaatc tatgcgcaag aaagcatacc
     8221 aatcactaaa caagatgatg tagggacgat gtttgaaaag cttagccttt taggaagaaa
     8281 gctattgcta gaaacattgc ccaatatttt ggatggacaa aaaccagtac ctcaagacga
     8341 gtcagaagca actttttcgc caaacatcac acgggaacaa gaagcaatcg attggcataa
     8401 aactgcagaa gaaattgaca accaagtacg cggaatgcgt ccttggccaa ttgcattcac
     8461 gacctatgag ggcatacgct ggaaattatt atcggttgaa gtattggcag aaactacaga
     8521 caaagcacct ggtacgatca tccggaaaga caaaaaaaat ctatggattg cttgcggtaa
     8581 acagaccgtc ttagccatca atgaattaca gccagctggt aaaggaaaac aggccgtaca
     8641 tgatttcctt aacggaagcg gtcagcaagt tcagattggt cagcaggtga aataatgaaa
     8701 aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg cattactgac attagagcga
     8761 gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg agatgatcaa acaaagcgag
     8821 ctaaatcaaa aagatgtggg tttatttaca gaattgatct atggtacgat tagccatcag
     8881 cgactattgg aattttacgt tgcaccattg atcgctaaag caaaaaaagt ggatgactgg
     8941 gtcaagatgc ttctttatct ttctgtatac caattagaat ttttagataa agtccctgca
     9001 catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa aaggaaatcc tgggacaggg
     9061 aaatttgtaa atggtgtctt gaggaattat cagcgtcaag gagcaccaga tctgcaaggc
     9121 attgctgatc ctatcgaaag actttctgtc aaaatcagcc tgcctctatg gatgacgaaa
     9181 cgactggtgg atcaaatcgg atacgaagaa actgaaaagc tcggtttatc gctatatcag
     9241 cctagccatg ctagtgcccg agtagataca cgccgacttt ccagagatca ggccatcact
     9301 gttttgcaag aagaagagat cacagctgaa aaaagccaag tcagtccata cggtatagta
     9361 gcgaaaaaag ggcatttagc ttctagctca ttgttccacg atggcgtcat gacgatccaa
     9421 gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg agcctgaaca tcatgtctta
     9481 gatgcctgtg cagcacccgg aggtaagacg acgcatattg cttctttttt agatccgtca
     9541 tgcggtggag aagtggtcgc tttagatgta cacgaacaca aagtcaaact cattgaagat
     9601 aatgcagcac gtctaggtgt ctcttctgct gtctacgctc aaaagatgga tgctagagaa
     9661 gtcagtaaac agttcgaagc agagcagttt gatcggattt tagtagacgc gccttgttca
     9721 ggtcttgggc tgcttcggcg aaaaccagat attcgttata aaaaacagcc ggatgaatta
     9781 gcgaatttac caaaaatcca attggaaatt ttagaaagct gcgcaccaac tttaaagtct
     9841 tccggtattc tgacttatag cacttgtacg atcttaaaag aagaaaacca agacgttgtt
     9901 tctgctttct tggaaagaca ccctgatttt gagaggattg atgttttgac caatcctatg
     9961 ctacaatcat caatcgaaga gaaaatgttg actttgtatc cgcatcagtt ttacacagat
    10021 ggatttttca tttgttgttt gcgcaaaaag taatgaggtg aaaaaatgca gattgaatat
    10081 caatcagatg taggaagacg acgtaacaca aatcaagact atgccagtgt atttaccagc
    10141 cgttcaggtc tcaagcttgc tgtattagca gacgggatgg gtggtcatcg tgccggagac
    10201 attgccagtc agatggcggt aacgaatcta ggcgaagctt gggaaaaaca agaagctgtt
    10261 gacgatgaaa aaatcgccca atggttcatt caatcgattc aagaagaaaa tatgctgatt
    10321 tatcagcgtg gacaagaaca gccggaatat aacggtatgg gaacaacaat cgtagcggcc
    10381 gctctgtcag atgactggtt cacgattgcc aatgttgggg atagcagagc ttatttgatt
    10441 cgtgaagata aaatcatgca attgacggag gatcattctt tagtcaatga actagtcaaa
    10501 tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa ggaaaaatat tttaacacgt
    10561 tctgtcggca tgcctggaac agtcgaagta gatgtttcta ctcatctttg gcaactaaaa
    10621 gatcgactgc tgttatgttc agatggcttg acgaacatgc tgtctgaaga agaaatagga
    10681 acgatcatca atcaagaagg cccattgtct cataaagtaa ctgagctgat tgatcgtgcc
    10741 aatgaagcag gcggagcgga taatatcacc gttcttctta tcgaatataa ggaggacagt
    10801 gtatgatcga gttgggcaaa aaattaaatg gccgttatca aatcactgga aatatcggta
    10861 gcggtggtat ggcaaatgta tttttggcgc acgatttaat cttggatcgt gatgtagcag
    10921 ttaaagtctt acgatttgac tttcaaaatg accagactgc gattcgacga tttcagagag
    10981 aagctttagc agcaacagaa ctcgttcatc caaacattgt tagtgtttac gatgttggtg
    11041 aagaagacaa catgcagtat ctggtgatgg aatacgtcaa aggaatggac ttgaaacgat
    11101 atatccaaac acattatcct gttccttatg aaacagcagt aaatattatg cagcagatcc
    11161 tttctgctat ttctttagct cataaccatc aaatcatcca ccgagatttg aaaccccaaa
    11221 acgtactgat cgataatgaa ggtgttgtaa aaatcacgga tttcgggatt gctatcgctt
    11281 tatctgaaac atcgatcacg cagacgaata cgatgctagg atctgttcat tatctgtccc
    11341 ctgagcaggc cagaggaagt atggcaacga agcagtcaga tatttatgct ttagggatca
    11401 tcctttatga gatgttgacg ggtagcgtac cgtttgatgg tgaatctgct gtaacaattg
    11461 ctttaaaaca ttttcaagat gatcttccat ctataaaagc acttgatcca aatgtgccac
    11521 aagcactgga aaacgtgatt ttacgggcaa cggctaaaga acctgcagac cgttataagt
    11581 cagccgagga aatgtctgat gatctttcta cagtattatc accagctcgt gcaaacgaag
    11641 aaaaatggca gcctcatgtg atggataatg aaacaaaagt tatcacaccc cttacggaag
    11701 atgcaccaat gcctgattca ttcaaatcta tgcctctgcc aaaagacaag ccagaaaacg
    11761 aaaccttacc agaggaggaa gaagttctgc ctgatcctaa aaagaaagga cagaaaaaat
    11821 ggtggctgat cctattagct attcttgctg ttttaggaat cggaacagct gtattctttg
    11881 cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc tgatctttct gaagcttctg
    11941 ctagagaaac actcgcaaaa gcgaaattag aagtagcaac aaaaacagaa gaaattgcag
    12001 atgacaaaat cgaagaaggt aatgtagtga agacagaccc ggcagcggga acgacagtca
    12061 agcaaaaacg cgaagtcact ctgtatatta gtagcggcac gaaaaaaatc aaattagatg
    12121 attacagtgg taaaagttat gaagatgccg aagcaagttt gaaagaatta gggttttctg
    12181 ctgacttgat tcaaaaagaa gaggaattca gttcagaggt agaaagcgga atgatcattt
    12241 cgcaatctcc atcagaagat acagaagtcg atcctaaaac agacacgatt actttcacag
    12301 tcagcaaagg accagaaaca gtgactttga gtagtcatgt aggagaaaac tacgaaactg
    12361 ttcgtaattg gcttgttaca caaggattca atatcaataa tattagtgct acttatgact
    12421 atagcgacac tgtaccatca ggttcgatca tcagtcagga tccagcctcg ggtgaagttg
    12481 tagcagaaga aacttacatc aattttgtcg taagccaagg aacagagcct aaattgaatg
    12541 atatctccgg ctatacaaaa agcgaagcac aaagttattt agctagtgtc ggtgcagaat
    12601 atatcgggca tgagacttcc gaatattcaa acacggtgga taaagataaa gtgattcgta
    12661 cagtaccagg ggcaggaacg acaattacca aaggaatggt tgtgaacgta atttactcta
    12721 aaggaccaga tccagcgtct tctagttctg aaaaagactc ttcagacagt tctagcagct
    12781 caagtagctc cagtagcaca agcaactctt caaaagaaga atcttccagc tccacaagtt
    12841 catcagctag cacctcagaa agttcaaaat aagtgctgaa taaaatatat cagaagggaa
    12901 tgcgacattg atttgttgca ttctcttttt tgtgtttcta tctcttttga tcaattttca
    12961 agccatatac tttgagaagt ttgcatggaa taacacctac tttcctcttc gtatggtaaa
    13021 ataaacaaaa acggaggtgg cacttatcaa aggacaaatc agaaaagcaa tcagcggttt
    13081 ttactatgta tacgcaaacg gagagaccta tcaaacaaga ggaagaggaa atttccggaa
    13141 tcggaaaatc acgccgcttg tcggagatca ggtgatcttt gagagtgaaa atcaaacaga
    13201 cggctatctt ctggaaatct tgccgagaaa aaatcaatta gttcgcccac cagtcacaaa
    13261 tgttgatctt ggaatcattg tcacaagtat cgtagagcct gcattttctt ataatctact
    13321 tgaccgcttt ctagttacct tggaatacga gtcgatcgaa ccgattattt ttttgacaaa
    13381 gaccgacttg tcagacgacc cagaaaaaat cgaagagatc aagcaaacct atactaaaat
    13441 aggctatccg gtgattgtac ccaatcatac gggtgatacg acagaactga tccgttactt
    13501 cccagaacgg ctatcggtct ttatgggtca gtctggagca ggaaaatcga cgctactgaa
    13561 tcaaatatca ccggaactga atttagcaac aggagagatt tctgatgcac ttggaagagg
    13621 acggcataca acacgtcatg tggaactgat tcctatctat ggcgggttag tagcagatac
    13681 accaggattt agctcgattg actttttgac aattgaagca ggagaactgc cgagacaatt
    13741 tcctgaattt gtagaagctt ctgtccattg caaatttaga gagtgcatgc atgctaaaga
    13801 acctggctgc gaagtaaaac atagagtaga aacaggtggt atcgcacaga cacgttacga
    13861 caattatttg caattcttac aagaaatcga aaaaagaaaa ccaatgtatg caaaaaaaga
    13921 aggtaaacga aaataggagg aaacaagaat gaagatagca ccatccattt tgagtgcaga
    13981 ttttgccaat ttacaaagag acattgagtt agtggaaaaa ggaggcgcag attacatcca
    14041 cgtagatgta atggatggcc gatttgtccc aaatatcaca ttagggccaa atattgttca
    14101 ggcaatccgt cctgttacta aattaccgtt agacgtccat ctgatgatcg ttgatccaga
    14161 aaactacatt ccagcatttg caaaagctgg agcagatatc attactgtcc acgtcgaagc
    14221 aactcctcat attcatcggg cacttcaaat gacaaaaagc ttaggagtta aagctggagt
    14281 agttatcaat ccaggaacac cagtctccat gatcaaacat gttttgcctt tggctgatca
    14341 agtactagtc atgactgtca atcctgggtt cggcggacaa agctttattg aagaaacagt
    14401 agaaaaaatt acggaactcg ctgatctacg tagagagaac aattggactt atgcaatcga
    14461 agtagatggc ggaatcgttc cagaaacagc aaaaatctgt aaagatgcag gggcggatgt
    14521 cttcgttgca ggatcatata tctatggagc cgaaaatcca gtcggacaaa tcgaacgatt
    14581 aaaagaggca ttagactaaa tgaaaatttt attagctgct ggaggtaatc cgcaaaactg
    14641 gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg atagaccgtg gtgcgctgta
    14701 cctgcttcaa gcaggctacc cagtagatct ggcagtgggt gattttgatt cattgtcgaa
    14761 cgaagagcag aaggaagtat ttgcaaaagc caaagagtat acccgttccc ctgcagaaaa
    14821 agacgataca gatacacagt taggtatctt aaaaacattt gaacgttacc cgcaagcgga
    14881 agtcactttg attggagcta cgggcggaag attggatcat cttttagcca atctctggtt
    14941 aggtatggaa cctcgcttca aaccctttgc ccatcagctt attatcagag attgtcaaaa
    15001 ttatttgtct tattatttgc ctggagagca tatagtaaga cagataccag atatgaagta
    15061 tttagcttat tgctgcttga ctcccgtctc gaatcttaca ttaaaaggca gcaagtatgt
    15121 attggaaaat aaagaagtag aatatccgac atcttatgca agtaacgaat ttctttcaga
    15181 taccgcagaa ttttcttttt cagatggtat gattgcagtt attcaaagca aagattgatc
    15241 cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg acagaatcta ttatttgcta
    15301 aaaaaaagct taagaggagc aacttacctt ttgtttcacg gcatttagtg ataaaattga
    15361 acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa gttcaatcct aataaaaata
    15421 ttattattac cttggttatt gttatcatcg tggtgacgat actaagtgtg accgctgcgc
    15481 agcgagcgaa tgaaggaaag cccaatttct tccaatccat cgttaatgac agtgttgcaa
    15541 tggttgataa aacaatctct gccccagtac ggtgggtacg aaatggagta gattctgtcc
    15601 ataatctttt ttcaacttat gccgaaaatg agcggctaaa agaaaaaatc gatagctatg
    15661 atgacgtttc acagcaaaac aagaattacg agagagaaat cgaagcgttg aaaaaagaac
    15721 ttgagttgaa tcagacgctg actagttacg aaaaagtaac ggcaaatgtc attactcgtt
    15781 cgcctgacag ttggcaggac atgctgatcg tagacaaggg ttctaaagac ggtatcgaag
    15841 caaatatggc agtcatgtcg caaaaaggat tgattggccg agtgatcgag gtcaatacgg
    15901 cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag ctccaatcat tttccagtac
    15961 gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa aaactatgat gaaaagctcc
    16021 atgccttagt ggtgacccaa ttaactggtg atacggatat caaagaaggg gatgttgtcc
    16081 agacatccgg tcttggaggg aattctccag ctaacttgcc gatcggtacg gttattaaaa
    16141 cgaaaccaga tagttatggg ctggatcggg aagtttatgt gaaaccttat gcagaaatgt
    16201 atgacgtgtc agttgtgacg attgtccaac ggctagcgga ggattagtcc atgttgaaga
    16261 aagaaacaat gaagtactat ctgccaatcg ttttgttctt tttgatgttg atagatggtc
    16321 atttaacaag aatgctaggg gagtggtcga aaggcaccta tatgtcaaat gcccactttc
    16381 tgatattggc attattatgt tgcagtatgg cgtttgaaaa acgttattta ctgattacca
    16441 cgattgttct cggggctatc tatgacgctt actatattgg cgttatcggt atctatgcag
    16501 tagctctccc tttaattgta tggttgatgt atgtaatgaa agacgttatc catgtcaaca
    16561 tctttactga atttttcagt atgatcatct ttgtcacggg ttatgaattg tttacgatgg
    16621 tggtccagtt gatttttaaa ttagcagtag taaataacac gtattttatt acaaggtttt
    16681 taggacctac actgctgttg aacatgatta tatttgtatt attcattttt ccctttaaga
    16741 aattattcag tgatgaataa tttctttttt tatttttagg tcgaaagcga aaaagaatcg
    16801 ttgttatctt aagaaatgct agacttaacg cggctttttc atgatcattt tctgaaacga
    16861 acagttttgt catttttaaa agttgttttg aaacatt
//
LOCUS       DACCZY010000058        16808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCZY010000058 DACCZY010000000
VERSION     DACCZY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.058"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(20..733)
                     /locus_tag="IVZ67_001616"
     CDS             complement(20..733)
                     /locus_tag="IVZ67_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8165696.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            908..2332
                     /locus_tag="IVZ67_001617"
     CDS             908..2332
                     /locus_tag="IVZ67_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8165697.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2631..3338
                     /locus_tag="IVZ67_001618"
     CDS             2631..3338
                     /locus_tag="IVZ67_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8165698.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3325..3648
                     /locus_tag="IVZ67_001619"
     CDS             3325..3648
                     /locus_tag="IVZ67_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8165699.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3737..4774)
                     /locus_tag="IVZ67_001620"
     CDS             complement(3737..4774)
                     /locus_tag="IVZ67_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8165700.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5095..6078)
                     /locus_tag="IVZ67_001621"
     CDS             complement(5095..6078)
                     /locus_tag="IVZ67_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8165701.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6493..7263)
                     /locus_tag="IVZ67_001622"
     CDS             complement(6493..7263)
                     /locus_tag="IVZ67_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165702.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7256..8191)
                     /locus_tag="IVZ67_001623"
     CDS             complement(7256..8191)
                     /locus_tag="IVZ67_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165703.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8507..10156)
                     /locus_tag="IVZ67_001624"
     CDS             complement(8507..10156)
                     /locus_tag="IVZ67_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8165704.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10273..12876)
                     /locus_tag="IVZ67_001625"
     CDS             complement(10273..12876)
                     /locus_tag="IVZ67_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8165705.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12888..13937)
                     /locus_tag="IVZ67_001626"
     CDS             complement(12888..13937)
                     /locus_tag="IVZ67_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8165706.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13963..14277)
                     /locus_tag="IVZ67_001627"
     CDS             complement(13963..14277)
                     /locus_tag="IVZ67_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8165707.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14293..14739)
                     /locus_tag="IVZ67_001628"
     CDS             complement(14293..14739)
                     /locus_tag="IVZ67_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8165708.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14740..16716)
                     /locus_tag="IVZ67_001629"
     CDS             complement(14740..16716)
                     /locus_tag="IVZ67_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8165709.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4989 a         3416 c         2764 g         5639 t
ORIGIN      
        1 aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat
       61 ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca acaactttgt
      121 ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca aattcattcg
      181 cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt tcatttgtga
      241 tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg actgctaatg
      301 gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt cgtacaatat
      361 tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc gatggaagtt
      421 caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg ctaaacaaac
      481 cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact tcagtcccat
      541 acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct ttctttactg
      601 ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt ttctcaccaa
      661 ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc atatacttcg
      721 caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata ctttaacata
      781 aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata tatatattta
      841 aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa caggaggtaa
      901 aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt ggggttccgc
      961 ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga ctatctggga
     1021 tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct ctattacaac
     1081 cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg ggcataactc
     1141 ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg gaaaagtaaa
     1201 tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata acggaatcaa
     1261 agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata aaggaggatg
     1321 ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt ttaaagaatt
     1381 tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta ttttaggtac
     1441 ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag cgcaagcagc
     1501 attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac tagctcttcc
     1561 tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc aaaatcctgc
     1621 tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt tagatcctat
     1681 ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata atcagcttcc
     1741 tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc aaattcttgg
     1801 actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg atacatctag
     1861 tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac gaatgaacga
     1921 ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg atatcaagaa
     1981 ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg cggatgaaga
     2041 acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt atatcaaaga
     2101 tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg ggtatcatct
     2161 ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat atgggctggt
     2221 ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat tttacaagca
     2281 attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat aatattctaa
     2341 aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc aataacaaat
     2401 actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca aatgtagtga
     2461 tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact acgtccagtc
     2521 ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag aatattcaca
     2581 agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta ttggatgaaa
     2641 agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt tatatcggta
     2701 ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg gtcgtcacgt
     2761 tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc atgcttgcta
     2821 gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc cgaatgattc
     2881 tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa aatctgctga
     2941 ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg aactatactg
     3001 gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat ttgacgtggg
     3061 tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg aaaaagtttg
     3121 gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa gtcatttctg
     3181 atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg attctggtat
     3241 atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca ctgattgggt
     3301 gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct gactatttta
     3361 ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact gaaaaaattc
     3421 gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat tatgtctgct
     3481 ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat caatctcgaa
     3541 aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag cttattggtg
     3601 atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat aaaaaaacaa
     3661 gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa
     3721 atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt cctaatatat
     3781 gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc ccatctgaat
     3841 agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca atgtgcgcta
     3901 aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac atatactccc
     3961 agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct ttgatcccta
     4021 cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca aagtagcggg
     4081 taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca cgaaaacttt
     4141 tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc agccattcaa
     4201 aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct agttgattca
     4261 aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg cggttggcat
     4321 tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc aatgaggtca
     4381 acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg acaatacgtt
     4441 cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct gcgtaaaaaa
     4501 cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact ttcccgactc
     4561 catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga agtttcgtat
     4621 cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa ctgggtacag
     4681 gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac ccttcataaa
     4741 aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag cttcatcaag
     4801 ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt tccactttta
     4861 ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga
     4921 ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa ctgaccctcg
     4981 gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg ttcttattac
     5041 ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     5101 ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc gtacttcatc
     5161 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     5221 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     5281 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     5341 cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca tgatgccatc
     5401 tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat ctccgttgat
     5461 cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag ggatgacttc
     5521 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     5581 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     5641 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa tcaattgtgc
     5701 cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     5761 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5821 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5881 tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5941 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     6001 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     6061 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     6121 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     6181 aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca caattccttt
     6241 tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac caaacgaaaa
     6301 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     6361 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacgaca
     6421 aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
     6481 acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc
     6541 gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg gcacatcgat
     6601 tgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat tgactttcga
     6661 aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac ctagataagt
     6721 taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact gagcaaaaat
     6781 cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg taatgatgac
     6841 catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga
     6901 gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga tcacatgcgt
     6961 aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag agaagaagga
     7021 cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac caggcacgat
     7081 gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca tttttccgaa
     7141 aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga cccagatacg
     7201 caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa
     7261 catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt tcttctaaac
     7321 gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc tgatcgaaga
     7381 ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca tcttcaaatg
     7441 tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca aagataaatg
     7501 tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc agttcacctg
     7561 attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa tgcgtcgtca
     7621 aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc tcgcgtctca
     7681 attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca
     7741 atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat
     7801 ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt
     7861 tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaagg
     7921 gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg actagatccg
     7981 tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa
     8041 tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca tagaaatctc
     8101 cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca taaacctttt
     8161 tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt
     8221 ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc gaaaaatcag
     8281 gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag tcagacaacc
     8341 ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa caaaaggagc
     8401 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     8461 caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat cccaatacat
     8521 gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct actaaagtct
     8581 ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa ggtccttctt
     8641 ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca aagtggacat
     8701 tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg acaaatgcta
     8761 aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc cgttcgatcg
     8821 tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg gaagccactt
     8881 ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg ccttcaaata
     8941 aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta aactgttctg
     9001 caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt ttgacatacg
     9061 tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag ttgaccggat
     9121 tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca ccgccctcag
     9181 taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt gcagcggcta
     9241 cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc ttttctaatt
     9301 tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg acatattcaa
     9361 aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat ttagggatgt
     9421 ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc agttttgcta
     9481 gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc tctgtttcta
     9541 gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg attgccaaat
     9601 agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga agcaagccaa
     9661 gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct agatattgtt
     9721 ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt ttttctctca
     9781 gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct tcaccaaaat
     9841 caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag gtactgttga
     9901 ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg atttcgctat
     9961 cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct tctttatcaa
    10021 atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt aatttcaaac
    10081 gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt aaaacgataa
    10141 attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt atagcactgt
    10201 tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt ttttttcttg
    10261 tatattacat atttatctct catttatcaa aattgacaaa taatcatagg gaggaattgt
    10321 tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa ggatagcctc
    10381 aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc cttgttcatg
    10441 aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa tcaatgaaaa
    10501 tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta acctgtgaat
    10561 aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt tttctgcctg
    10621 ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa aagtgacttt
    10681 atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt catttgtcgt
    10741 atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt cccctgttgt
    10801 tgaaaataag gttatgatta attgattttc tatacgttca tactctttta aaccatctgc
    10861 ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga atatttctaa
    10921 attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat caggggtatt
    10981 ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta aaacattaat
    11041 tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc cttcaacgat
    11101 ttgactttct cccagactga acgatatttc ataagatatt tcttccattt cgggtttttc
    11161 agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa ctaatgtttc
    11221 ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt ctgtatctcc
    11281 ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca aatgaacgaa
    11341 atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa aagaaagttt
    11401 atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta atttttcatt
    11461 atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat ttatttttat
    11521 gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa tcatccgata
    11581 agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg gactaccttt
    11641 gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac tataattctc
    11701 agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat cagccatccg
    11761 ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga ttcgatggaa
    11821 aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag cctggttttg
    11881 caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac catttctttc
    11941 tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt taaaattttc
    12001 tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt aaaccggctg
    12061 acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt ctacataaga
    12121 aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct ctacgacttg
    12181 atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa tatctttgtt
    12241 ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg caaattcagg
    12301 aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg gaaaattcat
    12361 ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg gaaccatcgt
    12421 ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat gcaaaagtgt
    12481 aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca ttggatctcc
    12541 atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg attcagcatc
    12601 cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc ctgttttcat
    12661 cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat cgacgattga
    12721 agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat ctagaacttc
    12781 agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc attctctgtc
    12841 ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta ctttccttct
    12901 tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc attgaccatt
    12961 aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat attcgtaaca
    13021 aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa aaatccattt
    13081 agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa aggatctttc
    13141 acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat agctgtcttt
    13201 gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc taaaaacatt
    13261 ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa gcttccgctt
    13321 gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc catatcaaac
    13381 gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga catgccttct
    13441 agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac ataccataat
    13501 atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt tagactaata
    13561 acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca agctaatcca
    13621 gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc agtgataaaa
    13681 gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga aatcatcggt
    13741 acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa tgtatccgtc
    13801 tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa ccctgctgct
    13861 acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat ttctcttcct
    13921 tgtttcttga attccattag taactccccc acgatatatt ttttatttgt taattaatgc
    13981 aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc ctactgttaa
    14041 aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg caaatatgac
    14101 aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca tagccccctg
    14161 tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat tagcttttgc
    14221 catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt ttgtcatatg
    14281 aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc tcgaatcttt
    14341 tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta aaatacgtaa
    14401 atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat caactggttg
    14461 atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca ctgttgattc
    14521 aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc ctgtggtcat
    14581 atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca caaacccatt
    14641 agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg catttaaatc
    14701 tatcactata ttatttatat tgattttttc actgatcatt tataagcctt ctttcaatat
    14761 ataaactaat ttttcaaacg acgaactcgt ttttaaattt gctaataact catgattcga
    14821 agcgatttca ttgaatgttt cataaatttt attcaattca ggttttactt tttcattaag
    14881 tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa tcaattcctt ctggattaac
    14941 gtagacacag attactggct tttttataaa atctacttcg atatgcggca tagcaaaatc
    15001 attcatcgct gttgaagata gtttttctct tttgattgct ccctctagta tcccttcttt
    15061 cgcgtaattt tcttttacca attttttggt aattatattt aaaacgttca cttgattatc
    15121 ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa aagcatcttc
    15181 tgtttgttca tttaactgta gatactgttt tacttttctg acatctgccc ctaacatcaa
    15241 aggactaaca acaataacag gaaaatttgt attttcaatc ggaatcgtgc ttataacaag
    15301 ttcatcagtg acttttctat cttgtagttc attgatgctt agtacatctt ctatctcaac
    15361 catatctgca aaaccgttat ttattctttg ctctaataat tttgatgttc catatccgct
    15421 actacaaact aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa gtgattgaat
    15481 atggagtgtt acgtaagcga tttcatcttc attaaaatgt atacagaatc tctcttcgat
    15541 ttcctctaat aaatctacac ttatcataaa tgacctttta aaagaatatt tgattttgtc
    15601 cgtataaggg ttatagatat ttactcccaa tttgagtctt ttaactgctg cattgagatg
    15661 taatactaga ttattgatca attcttcatc aggataaaat tcatttaatt taattcttac
    15721 taactcttgt agctcaacac ccatatctaa ataggaaagc ttcgtttgat ctaagtcgtg
    15781 attcatagca tttttttcaa tcgaagtaat gtgaatgtca acataagctt gttcatctat
    15841 tggcacaata attgaaaatg ctttttcaat cattttgatt aaaataagtg tattctttga
    15901 gatttcttct tcagaattat tactacgtga taagaaattg cttgtttcaa tcctttctat
    15961 cgcaatagca aggtggatag ctaaagtttg aaattcataa tctgtaaatt tcaattgtgt
    16021 cttattcatg tatcgattaa gcaattcggc tacttttttc aatatgtcct tatttaagaa
    16081 attattcgct tcaccaatga aattgatatt caagtctaag agatctgttt tactgttaga
    16141 tgctgtaaag ccaccccaat aatagttcac aacttctgac atcaaccttc ttttactttt
    16201 ttcatcagct ttgatgcgca cgccatttcg atctgaaact aaggacatcc catgcttgtt
    16261 tagtcttttt ctaacatctt tcattgtgct ttctaccgtc gacctgctca cgtataattc
    16321 gtcagcaaga ttttgaatct ttagagaatc attagtattc aataatttag caaacacata
    16381 agttagacgg tcctccggag agtctgcgat attgatttta tgactggata ataaatttcg
    16441 aagtgtttct ttttttccaa taagcgaaac gcctttttta ggttgaacca acatttgtac
    16501 atctggcatt ttttggatta tttctttcaa aattaaaata tctttgctaa ttgttttcgt
    16561 agatacatat aattgttctg ataattcttt gtaagttgtg tgctcatttt caatcaaaaa
    16621 gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc attatcttca cctcctgaat
    16681 acagcttaag tgtacaatta ttatctctat tttcatacag aagaaagttc ttgattaatg
    16741 tagaagtttc taatattctt gattatttca aatcaataaa aaagcctgca atatttatat
    16801 cacaggct
//
LOCUS       DACCZY010000059        16511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCZY010000059 DACCZY010000000
VERSION     DACCZY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.059"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..546
                     /locus_tag="IVZ67_001630"
     CDS             <1..546
                     /locus_tag="IVZ67_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP8165710.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IVZ67_001631"
     CDS             735..1403
                     /locus_tag="IVZ67_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8165711.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="IVZ67_001632"
     CDS             complement(1603..1833)
                     /locus_tag="IVZ67_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165712.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="IVZ67_001633"
     CDS             complement(1960..3342)
                     /locus_tag="IVZ67_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8165713.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="IVZ67_001634"
     CDS             complement(3498..4685)
                     /locus_tag="IVZ67_001634"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8165714.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="IVZ67_001635"
     CDS             complement(4692..5723)
                     /locus_tag="IVZ67_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8165715.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="IVZ67_001636"
     CDS             complement(5769..7193)
                     /locus_tag="IVZ67_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8165716.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="IVZ67_001637"
     CDS             7368..8273
                     /locus_tag="IVZ67_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8165717.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="IVZ67_001638"
     CDS             8364..9041
                     /locus_tag="IVZ67_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8165718.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9322..10218
                     /locus_tag="IVZ67_001639"
     CDS             9322..10218
                     /locus_tag="IVZ67_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8165719.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="IVZ67_001640"
     CDS             complement(10273..11643)
                     /locus_tag="IVZ67_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165720.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="IVZ67_001641"
     CDS             11881..12627
                     /locus_tag="IVZ67_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8165721.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="IVZ67_001642"
     CDS             12624..13463
                     /locus_tag="IVZ67_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8165722.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="IVZ67_001643"
     CDS             13464..14414
                     /locus_tag="IVZ67_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8165723.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="IVZ67_001644"
     CDS             14555..15307
                     /locus_tag="IVZ67_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165724.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="IVZ67_001645"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="IVZ67_001645"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8165725.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5371 a         3087 c         3319 g         4734 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc a
//
LOCUS       DACCZY010000060        16401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCZY010000060 DACCZY010000000
VERSION     DACCZY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.060"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1043)
                     /locus_tag="IVZ67_001646"
     CDS             complement(<1..1043)
                     /locus_tag="IVZ67_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165726.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQI"
     gene            complement(1030..2877)
                     /locus_tag="IVZ67_001647"
     CDS             complement(1030..2877)
                     /locus_tag="IVZ67_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165727.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2864..4501
                     /locus_tag="IVZ67_001648"
     CDS             2864..4501
                     /locus_tag="IVZ67_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8165728.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4646..5386)
                     /gene="truA"
                     /locus_tag="IVZ67_001649"
     CDS             complement(4646..5386)
                     /gene="truA"
                     /locus_tag="IVZ67_001649"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8165729.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5577..6212)
                     /locus_tag="IVZ67_001650"
     CDS             complement(5577..6212)
                     /locus_tag="IVZ67_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8165730.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6359..7600)
                     /gene="sstT"
                     /locus_tag="IVZ67_001651"
     CDS             complement(6359..7600)
                     /gene="sstT"
                     /locus_tag="IVZ67_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8165731.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            7798..8796
                     /locus_tag="IVZ67_001652"
     CDS             7798..8796
                     /locus_tag="IVZ67_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165732.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8809..10827
                     /locus_tag="IVZ67_001653"
     CDS             8809..10827
                     /locus_tag="IVZ67_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8165733.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(10887..11603)
                     /locus_tag="IVZ67_001654"
     CDS             complement(10887..11603)
                     /locus_tag="IVZ67_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8165734.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(11830..12372)
                     /locus_tag="IVZ67_001655"
     CDS             complement(11830..12372)
                     /locus_tag="IVZ67_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8165735.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12377..12604)
                     /locus_tag="IVZ67_001656"
     CDS             complement(12377..12604)
                     /locus_tag="IVZ67_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8165736.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12683..14563)
                     /locus_tag="IVZ67_001657"
     CDS             complement(12683..14563)
                     /locus_tag="IVZ67_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8165737.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14752..15366)
                     /locus_tag="IVZ67_001658"
     CDS             complement(14752..15366)
                     /locus_tag="IVZ67_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165738.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            15508..16068
                     /locus_tag="IVZ67_001659"
     CDS             15508..16068
                     /locus_tag="IVZ67_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8165739.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         5244 a         3101 c         2557 g         5499 t
ORIGIN      
        1 attatttgca agcttaagat ccgccaagga aattgtaaca atttggctgg cgtattttgc
       61 agaagatacc atggcaccaa actagttgta gctagccaag tcaaacacgt accataataa
      121 atcttttttt ctatgtttgt taatttttta aataaaatca ggggaataca taaagataac
      181 agaccaacta gtcccattga gtaagtagtt aagtcgttgt tcatagcttc agtcagacta
      241 tcgaacacat tagatgcatg agcatataaa tttgtcacgc taggtttgtt gattttttgg
      301 tagagcattt gttcaaacat cggataccaa aaagcactcg tcagaaccaa tgtcattcct
      361 gccgcactaa ataatttttt aaatatagca ataaaatctg cattcttgaa acctttagaa
      421 accaaacgaa taaaaataaa aatgataatt aatgctgtac tcattaatgt tgatagtaca
      481 tggctgtaca ctagcaaact catactcaat gctagcttta cagacggata cttttcttct
      541 tttccagaga tgatttgata agcataaaat aatacaggga taacaagtgt tattgcgata
      601 gcttcggcaa ttgcggaacg atagtagatg tctactgtcc gataagcaga gaaaatatag
      661 agacaactaa ataaaaatgc cgcttttttt tgttttaaaa accgctcgcc ataatgataa
      721 gctacgagtg ttgtacacaa gttcaataac caaacgtaaa taatatagct tacaatcaaa
      781 ttttttgtca tccaatataa cattacagct ggaaaaaaag tcagaaaggg ataaaaataa
      841 tttacaccat atccagtata attgaatgtc tcataattaa tcggactggt aaaaatagtc
      901 gataacccct tgattcgaga taagtgaaaa gaaagatctc cagtggtata aatactctgt
      961 gtatttttga aaaagatgaa agaatatata ctactaacta taattaatcc actcaatatt
     1021 actacttttt tatttatttt cattcttctt gtttatctcc tttttagtca catatcgaga
     1081 atctttcaac ttattaggtg caaatcgttt aaaacttgaa tacaccacta aacaataagt
     1141 cagaatactg atccataaag ccatgagagc agttggagta tattttgttg taatttctat
     1201 cttattttct ccagcattta catccacatt cactaagcca tgttttttat aagagtcatt
     1261 tacgacttta ccgtttactt ttacctgtgt acctaaataa gcatatactg gtactgtcac
     1321 tttttgcttt ttgttgcttg ccactgtgta agtaatgtta ttatctgcag ctttatactt
     1381 ccctttttcc cacgattctc ctacttttac ttcatgtttt tctaagtgag gcctttcttt
     1441 tatcgcttct gttggggcgt aatcgtataa tcctcccata tctgatgttg tataacgtgg
     1501 gagtgtttct tcattgataa caatatgacc gtgttttgat tggataactt ttgcgtaatt
     1561 ttcttttgct gctatagacg tgacgagtag aaacaccaca gtagcgccaa tgattaacca
     1621 tgaatatttt ttattcatag tctttttcat aaaaacaagc gttatgatca ttgaagaaaa
     1681 aaagatttgg atacctaaga tacgccaagg aaactggagt aagcttatcg gtgttttttg
     1741 aaacaaaaac catggaaata aattagttac gcatacccat gtagaaacta cgccaacata
     1801 gatgattttt tcttttttct ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac
     1861 caatcctaat aaccccatag tgtatgttgt aatatcgtta tttaacgctt gaatgatact
     1921 atctgaaata gaaagtgctt ctagctgcaa atctgttttg aagggactgt gtatttcttg
     1981 ataattcatc tgctgcaaca taggatacca aaaatagcta gttaatgcac aagtcaaaaa
     2041 aacagccttt actaaattca cgaccacaaa ttttatatcg ttttttattt gcttgcttaa
     2101 aaacaaacga actataatca tacaagcaat caatgcacta cacaatagtg tagttaagac
     2161 atgagtatac acaagtagac ttatactgac agataaagcg atgacttttt tttctctatt
     2221 gaatatcaat ccataagcat aatataaaac agctgggata atcgtaagag cgattgcttc
     2281 tgcaattgcg gatcgataat agatatcaac tgttcgataa gttgcaaaag tatataaaca
     2341 actaaataaa aaagcagcat ctatcttttt aaaaaacttt tctccataat aaaaactaat
     2401 cagaatcgta caaatattta ataaccaaat atatagaaca taacttaata ttaagttatt
     2461 tgaaattcca taaaaaacga ctgcaggaat tattgtaaga aaaggataaa aatagttcag
     2521 tccatctcca taattgttaa atgttgtata gttgattggc ccttcaaaaa tagaagataa
     2581 ccctttgatt cgtgaaagat gaaaagataa atcacctgtt gtataaattt cttgagcatt
     2641 tctcataaaa tttactgaat ataaagtact aagtaagata agaactacta aaagaaatgc
     2701 ttttttactt attttcatag atttgttgtt ctcctttttt aattctattt tcagtatact
     2761 agaattgtct ttttttaata agttttgcgt taaatttttc atagcactag tatgatatac
     2821 tagaaaagaa tcaattttta ttacttttaa ataggagaaa aaaatgaaaa aaaacaaaaa
     2881 ccaaataata gatatcattt ttatgttatt tctaagtttt gcttacgtca tccctctatt
     2941 catgtctaag ggaatctatc atagtgtcaa tcaggatact tactttcatt tatctcgaat
     3001 cataggacta gataatgtat ggagttctcc agtaaacttc aataacttcg atcatcatgg
     3061 aacaatgatg aatatttttt atccatggct tactctttat ccagcttttt tattttataa
     3121 aatgatggga aatcttgttt taagttataa tatctactat ttttttatca cttttttaac
     3181 aatggtcgtt tcttattttt ctatgaaaca aataaaaaac aatcgttata tttcgctgtt
     3241 attttcgatt atttatacat tttccgccta tcgtgccatt gacatattcc atcgtgcttc
     3301 attgggagaa gctgtcgctt taacattttt gcctttgata ctaatggggt gttacgaaat
     3361 ttatattaga gattaccaaa aatggtactg gttgtctatt ggcatgacgc tagtagtgta
     3421 tactcattta ctttctgtcg ctatggtttc tgtatttatt ggaggaacgt tgtttctttc
     3481 tttttatttt tgggaccaaa aaatcgctag actcttaagt cttttaaaag ctaccgtcct
     3541 cacctttttt ttaagtgcgg gattcttaat tccttttatt caacaatcta gagcacaaga
     3601 attgaaagtc ccactgggaa aagaattaag tggtatggct cctagtgata tgctgacaca
     3661 tattttaaat aataactata acaactatac tattggatta ttcttatttc tcggattgat
     3721 tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt tctttcttgg
     3781 tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta accatacacc
     3841 agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat ttatagccta
     3901 tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat ggaaaataat
     3961 ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt ctatccatgc
     4021 taacaaagat tcattaacac ttatagcacc tgaagacgct gttgctatag ctgaaaatta
     4081 caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga ttaataatga
     4141 tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga gtaacaaact
     4201 tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac ctatttatcg
     4261 ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat tatctaagtt
     4321 tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta cttatcagta
     4381 cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt tatttttact
     4441 ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac attctcacta
     4501 gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa agtctttttt
     4561 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
     4621 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
     4681 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
     4741 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
     4801 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
     4861 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
     4921 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
     4981 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
     5041 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
     5101 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
     5161 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
     5221 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
     5281 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
     5341 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
     5401 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
     5461 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
     5521 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
     5581 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
     5641 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     5701 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     5761 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     5821 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     5881 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     5941 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
     6001 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     6061 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     6121 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     6181 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     6241 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     6301 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     6361 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     6421 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     6481 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
     6541 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     6601 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     6661 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     6721 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     6781 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     6841 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     6901 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     6961 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     7021 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     7081 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     7141 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
     7201 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     7261 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     7321 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     7381 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     7441 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     7501 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     7561 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     7621 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     7681 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     7741 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     7801 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     7861 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     7921 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     7981 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
     8041 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
     8101 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     8161 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
     8221 ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc gaaaacaaca
     8281 attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac tctatttaat
     8341 aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta tttattttta
     8401 atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa gcttcctcaa
     8461 gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca aatttcactc
     8521 atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc tctcttcgca
     8581 atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa attaggtata
     8641 aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat atgtaatttc
     8701 cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa ctcagggaag
     8761 gcatggctga taaatatcgg gcagtttttt atttgatatt tattagcagt gtccctccta
     8821 gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat cgaatacttc
     8881 gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca ttggcttttt
     8941 gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt cgtgcagatt
     9001 ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt aaaaatattt
     9061 actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt tttcttctta
     9121 ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat cattaactta
     9181 ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt ctttccttca
     9241 gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc agtcttatta
     9301 atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt acaacgtaga
     9361 ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag tatcgctgta
     9421 ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc ttctttcttg
     9481 ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc cgtaaaagat
     9541 aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga agaacctgct
     9601 ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa ggcagatgaa
     9661 atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa tttaagtgaa
     9721 agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc tattccttac
     9781 atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg ttatggtggc
     9841 ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa cctgtctgct
     9901 tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc aaatattacc
     9961 gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta tcgcagaaaa
    10021 caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc ggataaatta
    10081 acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc atacaaagaa
    10141 accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt atccactatg
    10201 caaaaccata tgccttatga tgattactat agtgaattga actacactgc tgaaggaact
    10261 gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct tcactatacc
    10321 gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc gattaccttt
    10381 gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa aaaatatgga
    10441 ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg tgaacaagca
    10501 gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc tcttgagcat
    10561 gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa tcatctacct
    10621 gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa acaggtcttt
    10681 gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca aaaagaaatt
    10741 ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta cagtgcaaca
    10801 tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg tgataaaaga
    10861 catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat ttgattaaag
    10921 ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc gggattcccc
    10981 tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa tagatagacg
    11041 tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat ttgatagtct
    11101 gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc aatagccatc
    11161 ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc cacactcatt
    11221 tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt cataaatgcc
    11281 tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc gctgctttct
    11341 gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    11401 gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc tgcagttgac
    11461 ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt ttcttttgtt
    11521 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    11581 gttactaaaa tccttttttt caattaaaaa actcctttcg attactccca cttctttatt
    11641 ctatcaaata agcaatgtga ttttattgat ttttcgtatt ttctcatcaa tctttcaaat
    11701 cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa tacatcagcg ggtgcttctt
    11761 ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta tttgaagaaa
    11821 catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag tttttccttg
    11881 gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa caagcaattg
    11941 ttgcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga taaagacatt
    12001 ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta aatatttgtt
    12061 ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag acggaacgat
    12121 ttcattttca atcactaact catgagagat tttattgaac catctcgctt cttgatcagc
    12181 atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat acaattgtgc
    12241 ttggatcaat ttcatttctt ctaatttaaa atttgctaat acatgcccca ccatggcgcc
    12301 agcagttggt ttatgatggt cgatatctgc ttgttttgtt tcttgaacaa atttttcttc
    12361 cactatattc attgatttac gcctctttta cttttgctga ttggacttca taacctaatt
    12421 tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag tttgctttga
    12481 ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga gaaaccgcat
    12541 gttcgatttt ttgcatacag cttggacaag ctagtgtatc taattgcata ataatttttt
    12601 tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca gcttccataa
    12661 aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt ccaaaataca
    12721 ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg aacatcccac
    12781 tagccatgta aataaacccg gcaaataagc cgttgagtaa aaataagacg acaccaatgg
    12841 caatcactac attttcacgg gtatttaaaa cagttttctt agccaagcgg taggcatgaa
    12901 ccaacgattc aaaattagat tgcatcaaga caacgtcact cgtttcgata gcaacatccg
    12961 ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa tcattgattc
    13021 catctccaat aaaagcaacg cgcaatcctt gttcttggta ttttttcaca aattgaacct
    13081 tttcgtctgg taataattca gcatggactt catcaatgcc taacatatca gctacataat
    13141 cagcagtcat ctgattatca cctgtcagca tcactaactg ttttgctcct gcttgtttca
    13201 acttagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacct atgatttgag
    13261 tgattttttt gtcgattgct acaataatga ccgagctgcc tgtttgttgt aattgatgta
    13321 aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat ttggtgttgc
    13381 ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg attgtctgat
    13441 tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa tggatcacgg
    13501 cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct aagctgtccc
    13561 cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg gtcaatgtac
    13621 cagttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct tcaccgcctt
    13681 tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa acaggcgcac
    13741 caatgactaa agcgccagga caaccaagaa ccaaaacagt gattgctaaa cgaaaatcac
    13801 gactaatcag accaacaacc aacgcaataa gtaaaacggt tggagtataa tactgagaaa
    13861 atcgatcaat gaatttttcc gcatgggatt tggtatcttg tgcttcttct actaattcga
    13921 taattttggc aaaggtcgta tcatctccga ccttagttgc ttcaatcgtc acgtagccat
    13981 ctgctaaaat cgttccagag aagacttcat ctccactgat tttgacgact tgtcgtgatt
    14041 caccagtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt ccgtccacag
    14101 gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca tcgatttcca
    14161 cttcttcagt cgttccatct tcttggacaa cactagcagt agatggggcc atctcggtca
    14221 aagccttgat tgactcccgc gttttggtca atgttttttg ttccagatag ctaccaaaca
    14281 aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag gcaccaagca
    14341 cagcaatagt aacgagtaaa tcaattgaga taactttgac tttcactgcc tgatacgcat
    14401 ggatcataat aggtatgacc gcaataatag aagctatggc tagagcaatg ttatagaaca
    14461 agacaaaacc agtaaatttt ccgataaaac caaaaataat caacacagct gataaggcgg
    14521 tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc cctcttttcc
    14581 tttcattctt acgagactaa gtatagatac ttatccatca taaaaaattg accactatca
    14641 agtttagtca aaaaggcggt tttcttttgg tagaatacac tacaaaaaat agttagcaag
    14701 aagactcact aaaagtgatc ttgccaacta ttgtcgtttc tatcgaaatt ttcattgaat
    14761 gattttcctg tacagcatac atcggagatc tatttcttta tctaagattt ttatcttgct
    14821 aattttttcc tttagtcttt caaatccacg tcgttcatag aattgatacg tacagccact
    14881 atctgtaaaa aggaatattt cttttccttt ctctcttttt tcaaattctg ttagcaactt
    14941 ggtaccgatg ccttttgttt tcaaatcagg atttgcagct aaaaaaatga tctctccatc
    15001 tggcgaatgc ttctttaaat atgctgcaag catttgttca tttgctgtct cgtaagctcc
    15061 tttactttct ttagcaaata tattttgtaa aaaatcgaat atcttaacat atatttgctg
    15121 tccaaaagaa cgatgtttct ttttttctcc ttttatctcc gccagcaaaa cacctgccaa
    15181 ctcgtcctct gcatacaaag caagaatttg agttgctcga ttcaactcca tatacaaaaa
    15241 atatcttccg taaagattca gcagaagttc attttccata tatctctcaa agttcattcc
    15301 ttcaacagca aaatagatag cttttttgta atctttcttt tctaagggtt taaaagttag
    15361 tttcatcatt tcgcctcctc aacttcaatg attgtgttat acttacatta taatagacac
    15421 agtgtctact atctataatc aatcatctat aagtttgtcg attaagcgac agctttgctt
    15481 tgatatgtcg gataaggaga taggtaaatg aaaaagacag atagacgcgt caaaaaaaca
    15541 gaaaaagctt tggctgaaac actatccaca ttattagtaa ataaaaagat tcaagcaatc
    15601 actatacgcg aactaacaga aacggcagat gtacatcgtt cgacttttta tacacattat
    15661 aaggatatct atgatctata tgatcaatta gaaagcaact tttttcggga tctaaatgaa
    15721 attctctctt atgatccagc ccatagctat gaggaactat acactcgctt gattgactat
    15781 gtttacgcaa atactttttt atatcgctta tttatcctca accatggtga tatttcgttt
    15841 cagaagaaag ttaaacaagt tattgaagaa aactatctga acatatggct atatgaagac
    15901 aagaaaacga ttatcacaga agaaatgaga ttctttacta cgtaccacgt ccaaggttgt
    15961 ctatccgttc tcaatcggtg ggtagaaagc aattacaagt actccaaaaa agaattatta
    16021 gaactcttac agaaactgaa tgatcacata gagaaaatca tgccttgaat aaaaacatac
    16081 taagcttagt taagaagaac cgtaaccttc tcgtataaca actgaatctt atttagccaa
    16141 attgtttact gaagattttt tgagtaaaat ttctataatt cctacttatt gaaaagtagt
    16201 ttgtagtgct gcttctccct ctctaagtga tatatcttgt tgtaccaatc aaattcacct
    16261 catcattaat aaattacttt taggctaatt tattacaact tataggttga agctgtaacc
    16321 aatttatagt catattatat ttttatcatc cagtttattg aattcttctc gtttacctca
    16381 attcattttc aaggggaatc a
//
LOCUS       DACCZY010000061        16291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCZY010000061 DACCZY010000000
VERSION     DACCZY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.061"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(240..1967)
                     /locus_tag="IVZ67_001660"
     CDS             complement(240..1967)
                     /locus_tag="IVZ67_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8165740.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1960..3153)
                     /locus_tag="IVZ67_001661"
     CDS             complement(1960..3153)
                     /locus_tag="IVZ67_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8165741.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3340..3984)
                     /locus_tag="IVZ67_001662"
     CDS             complement(3340..3984)
                     /locus_tag="IVZ67_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8165742.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4078..4212)
                     /locus_tag="IVZ67_001663"
     CDS             complement(4078..4212)
                     /locus_tag="IVZ67_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8165743.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="IVZ67_001664"
     CDS             complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="IVZ67_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8165744.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6343..6816)
                     /locus_tag="IVZ67_001665"
     CDS             complement(6343..6816)
                     /locus_tag="IVZ67_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8165745.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7217..7441
                     /gene="nrdH"
                     /locus_tag="IVZ67_001666"
     CDS             7217..7441
                     /gene="nrdH"
                     /locus_tag="IVZ67_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8165746.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7600..9759
                     /gene="nrdE"
                     /locus_tag="IVZ67_001667"
     CDS             7600..9759
                     /gene="nrdE"
                     /locus_tag="IVZ67_001667"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8165747.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9809..10774
                     /gene="nrdF"
                     /locus_tag="IVZ67_001668"
     CDS             9809..10774
                     /gene="nrdF"
                     /locus_tag="IVZ67_001668"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8165748.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10863..12002)
                     /locus_tag="IVZ67_001669"
     CDS             complement(10863..12002)
                     /locus_tag="IVZ67_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8165749.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12311..13024)
                     /locus_tag="IVZ67_001670"
     CDS             complement(12311..13024)
                     /locus_tag="IVZ67_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8165750.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="IVZ67_001671"
     CDS             complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="IVZ67_001671"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8165751.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14213..15745
                     /locus_tag="IVZ67_001672"
     CDS             14213..15745
                     /locus_tag="IVZ67_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8165752.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15936..16115)
                     /locus_tag="IVZ67_001673"
                     /pseudo
     CDS             complement(15936..16115)
                     /locus_tag="IVZ67_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16135..>16291)
                     /locus_tag="IVZ67_001674"
     CDS             complement(16135..>16291)
                     /locus_tag="IVZ67_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8165753.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT         5044 a         3371 c         2854 g         5022 t
ORIGIN      
        1 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
       61 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tgaacgtttc
      121 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
      181 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
      241 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
      301 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
      361 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
      421 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
      481 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
      541 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
      601 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
      661 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
      721 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
      781 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
      841 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
      901 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
      961 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
     1021 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
     1081 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
     1141 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
     1201 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
     1261 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
     1321 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
     1381 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
     1441 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
     1501 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
     1561 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
     1621 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
     1681 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
     1741 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
     1801 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
     1861 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
     1921 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
     1981 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
     2041 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
     2101 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
     2161 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
     2221 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
     2281 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
     2341 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
     2401 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
     2461 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
     2521 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
     2581 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
     2641 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
     2701 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
     2761 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
     2821 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
     2881 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
     2941 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
     3001 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
     3061 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
     3121 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
     3181 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
     3241 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
     3301 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
     3361 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
     3421 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
     3481 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
     3541 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
     3601 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
     3661 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
     3721 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
     3781 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
     3841 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
     3901 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
     3961 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
     4021 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
     4081 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
     4141 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
     4201 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
     4261 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
     4321 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
     4381 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
     4441 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
     4501 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
     4561 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
     4621 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
     4681 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
     4741 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
     4801 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
     4861 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
     4921 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
     4981 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
     5041 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
     5101 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
     5161 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
     5221 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
     5281 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
     5341 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
     5401 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
     5461 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
     5521 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
     5581 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
     5641 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
     5701 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
     5761 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
     5821 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
     5881 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
     5941 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
     6001 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
     6061 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
     6121 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
     6181 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
     6241 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
     6301 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
     6361 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
     6421 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
     6481 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
     6541 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
     6601 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
     6661 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
     6721 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
     6781 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
     6841 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
     6901 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
     6961 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
     7021 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
     7081 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
     7141 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
     7201 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
     7261 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
     7321 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
     7381 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
     7441 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
     7501 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
     7561 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
     7621 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
     7681 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
     7741 atgacttttc catctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
     7801 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
     7861 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
     7921 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
     7981 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
     8041 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
     8101 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
     8161 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
     8221 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
     8281 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
     8341 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
     8401 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
     8461 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
     8521 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
     8581 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
     8641 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
     8701 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
     8761 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacagatatc
     8821 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
     8881 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
     8941 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
     9001 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
     9061 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
     9121 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
     9181 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
     9241 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
     9301 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
     9361 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
     9421 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
     9481 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaac ggcacaacaa
     9541 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
     9601 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
     9661 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
     9721 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
     9781 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
     9841 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
     9901 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
     9961 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    10021 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    10081 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    10141 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    10201 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    10261 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    10321 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    10381 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    10441 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    10501 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    10561 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    10621 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    10681 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    10741 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    10801 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    10861 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    10921 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    10981 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    11041 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    11101 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    11161 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    11221 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    11281 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    11341 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    11401 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    11461 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    11521 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    11581 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    11641 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    11701 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    11761 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    11821 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aattgccgaa
    11881 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    11941 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    12001 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    12061 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    12121 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    12181 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    12241 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    12301 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    12361 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    12421 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    12481 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    12541 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    12601 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    12661 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    12721 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    12781 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    12841 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    12901 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    12961 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    13021 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    13081 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    13141 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    13201 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    13261 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    13321 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    13381 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    13441 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    13501 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    13561 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    13621 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    13681 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    13741 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    13801 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    13861 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    13921 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    13981 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    14041 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    14101 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    14161 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    14221 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    14281 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    14341 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    14401 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    14461 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    14521 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    14581 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    14641 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    14701 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    14761 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    14821 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    14881 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    14941 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    15001 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    15061 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    15121 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    15181 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    15241 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    15301 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    15361 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    15421 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    15481 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    15541 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    15601 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    15661 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    15721 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    15781 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    15841 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    15901 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    15961 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    16021 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    16081 gcacctccaa taaagtacat aaacataaat acagacccgt ggtgctagtt aattttaata
    16141 tcctaaagaa tatctcaaag ttccttcaaa acgtcgttgt gctcgactta atagaacact
    16201 gtaagccagt aatatgcttt caaatctact ctctagccct ttaacagaac gtgagttgaa
    16261 attttgacaa cctaattttt ctaaggaaga a
//
LOCUS       DACCZY010000062        16249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCZY010000062 DACCZY010000000
VERSION     DACCZY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.062"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..173)
                     /locus_tag="IVZ67_001675"
     CDS             complement(<1..173)
                     /locus_tag="IVZ67_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165754.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVZ67_001676"
     CDS             complement(593..1714)
                     /locus_tag="IVZ67_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8165755.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVZ67_001677"
     CDS             2042..3451
                     /locus_tag="IVZ67_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8165756.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVZ67_001678"
     CDS             3448..4176
                     /locus_tag="IVZ67_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8165757.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVZ67_001679"
     CDS             complement(4304..5215)
                     /locus_tag="IVZ67_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8165758.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVZ67_001680"
     CDS             complement(5232..6239)
                     /locus_tag="IVZ67_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8165759.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVZ67_001681"
     CDS             complement(6236..8365)
                     /locus_tag="IVZ67_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8165760.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8495)
                     /locus_tag="IVZ67_001682"
                     /pseudo
     CDS             complement(8370..8495)
                     /locus_tag="IVZ67_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8666..9853)
                     /locus_tag="IVZ67_001683"
     CDS             complement(8666..9853)
                     /locus_tag="IVZ67_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8165761.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(9946..12291)
                     /locus_tag="IVZ67_001684"
     CDS             complement(9946..12291)
                     /locus_tag="IVZ67_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8165762.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            complement(12278..12667)
                     /locus_tag="IVZ67_001685"
     CDS             complement(12278..12667)
                     /locus_tag="IVZ67_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8165763.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            complement(12717..13613)
                     /locus_tag="IVZ67_001686"
     CDS             complement(12717..13613)
                     /locus_tag="IVZ67_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8165764.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            complement(13616..15463)
                     /locus_tag="IVZ67_001687"
     CDS             complement(13616..15463)
                     /locus_tag="IVZ67_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8165765.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            complement(15560..16060)
                     /locus_tag="IVZ67_001688"
     CDS             complement(15560..16060)
                     /locus_tag="IVZ67_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8165766.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(16073..>16249)
                     /locus_tag="IVZ67_001689"
     CDS             complement(16073..>16249)
                     /locus_tag="IVZ67_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165767.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
BASE COUNT         4915 a         3396 c         2675 g         5263 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca
     8821 accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa
     8881 tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat
     8941 cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg
     9001 ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat
     9061 caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct
     9121 tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca
     9181 aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt
     9241 gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata
     9301 caccttttga gacaatacga tgattttccc ttactttcat atacagggca tcacacatga
     9361 aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca
     9421 actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt
     9481 caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag
     9541 cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt
     9601 ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt
     9661 agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat
     9721 attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg
     9781 tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat
     9841 gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact
     9901 cctaactgag atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat
     9961 cccgttttcc aaatctcgca ggcgtttttg attgctttga tcctcctgat ccaaaccaaa
    10021 gaattccaag gttttcttga tttcatgaat atcggtactg cgaaaagcaa acttcaaccc
    10081 gatattattt tttaaacgtt catctaataa atcgtcgcta ttttgagtga cgaaatagac
    10141 cccagcattc atggcccgac cagcacgaac aagcttattc gataacgttt ttccttgagc
    10201 cacttgaaga aaactccaag cctcatccaa atcgacaatt ttgaagattt ctcggttact
    10261 atggataaag tccaaggcaa aagtggaaat gacgattagc atcgaaacac taagcaattc
    10321 catggtggta tattcttcaa aagtcgtttc tgcatccggt aaaaccaaat ccgccacttg
    10381 aatgatattt agctgtttat ctaaactaat cgattgggta atcgagccgt ctgagaaaag
    10441 caaatgagca aaatcataat cggtaaagga ctcgatatga tcggcgatat tttccgcgat
    10501 tggtgacccg tcttttcgca attcgttaat aacttgtaat aacccccgtt gatcactttg
    10561 agtcaccgtt cgaatggctt tacgtaacac aggaaacttc tccccgtctc gacttgaaat
    10621 tcctgttaaa aaagtcagaa tatcaattgc taaactttcc gagtctttct ttttcttcat
    10681 aatcacataa ggatcaagca gaccttgatt ttctgactta ctcgtcaagt tgaggatact
    10741 gatttcatcc gcaatttctg gcaaggtttc tttccaccct cctcgctccc cttttggatc
    10801 gacaatcaca gcttgaccac cgaacaaaac agcatagtag actaagagat tgttactaaa
    10861 ggatttacct ccaccaagag aacccaggaa agcggcggcc agcgcattgg ttactgaacc
    10921 cttgactcct tgtgaagcca atgccggttt caggtaaaca ttacgacctg tatccacatt
    10981 gtagccaaag taaatccctt ctaattctcc tagcatctga gtagccccaa aaccaagccc
    11041 cgccaaaaaa tcagacgtaa catactgaat atagtcgttc atatatcgtt tacttgaggg
    11101 taagaattct tcatgtagcc ccatcatatc gccaaagggc cggactagtt ttacattggt
    11161 atcatcataa aaatcgagca cttcatcaca gcgtcgcttt agttcatcca ctgattcggc
    11221 actcactcga accacataac tcagtttgta catagattct tttgactgat ccaaggtggt
    11281 ttccaattca tccacggaat ccaaagcatc taagacattg gaattcgttt cattgtcaga
    11341 ttggtaagca tgattgtcta aatctttcag ttcttttttc ttattacgaa cggtagctaa
    11401 agccttttta ttcgtcacaa tctctacatt catacttgtg tcgattggga atgtaaattg
    11461 ttgttgctgg taatagaaaa gttctgagga aggaaattcc atctccccca caattgtatt
    11521 aattgttaaa tacgctacgt acgactcatg attctcatgt tccatccgta agcaccttgg
    11581 tttttcttca attaaacaac gatttggacg taataagtca taacgtttca ccagtgtttc
    11641 tgattttagc tttttctttg gtagctgaaa ttcgtattct tcaaacggta tccctttttc
    11701 gccataaata tgttcaatca aatacgtcag gtcactcggt gtgactcgcc tgactttaaa
    11761 tcttcgagcc aatttgcttt ccattaattt ttcaagctta gaatagcagc gaatttcttc
    11821 attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga caaattcttg
    11881 aagcccagaa aagaaagatt tcttgagact ctttagattg acttcttcat ctgtcgcaat
    11941 tagtttaaag ccaataaaaa agcgataatc aatctgacta tcgccaatca tggatactaa
    12001 tgcatctgtc tgtatatcaa ttcgttgttt cgctacttct ttgagtctac cggtaatttc
    12061 tttttttgat cgttcttgtg tggcgcgaat actactttct gtggcaattt gtaacgcatg
    12121 gatcttgcct tcacgatttt gcgcaattag ttggcgaaag ttatcatgca cttgaaattt
    12181 ctgttcgggt gacaaaaagg agtaattgta aggcactaat tcatagtagg caaaacactc
    12241 gccttcttgg ttaaagacga gattattttc aatatactta atcggataca tagttcacac
    12301 tccttacaag agtactgggt tcatcccatc gtttcttctg ttctttgact ttctttcctg
    12361 caaaagtcac cttaaatcgc aagtgatagc tgataaagga tcgtaaaaat cccagtggtt
    12421 tcttgccatc aaacgtcttt tgcgacataa accaagtgac agccaccggt atgcctaagt
    12481 attttaaaaa gacctcctct atcatagata gtggaggcac atgggcaaat acgatgacaa
    12541 acagtaaaga taaaacgaac caagccattt ggttaaaagt cacaggaaaa ggcaactgaa
    12601 aatcattgat tgcgtaaatg actttttcaa ctgcccagat actggtatag ctttttattt
    12661 ttttcatttt tcctcctaaa aagggcggac ataataatat gtctcaccct ctagtgttat
    12721 tctacaattt gtttaaaacc atagcgtttc gctctatttg taaaagcatc ctcgccacct
    12781 tccatcacag tacatattct tggcttgata ttgggttcat ctaccgaccc gcactgatca
    12841 gccgatattt cttttgaatc tcccatgcta atcaaccatt ctgaatcacc attaactggg
    12901 atatttgcat tatgagttac aattaaaatt tgtctgtttt ccttttttgt aatgatactt
    12961 cttacgatta agttattaat taattgacta tccaaatcat cttcaggctg atccaatatg
    13021 agtggtgaat ttccatacgc caaaatcata gaaagaattg cagatgtctt ttgaccagca
    13081 ctagcattcg ataaactaac tttttttcca tttagttcaa gtcggataac tactaaatca
    13141 tttggcttaa gggtttgtaa ttcaagaact tgtgcatcat tcaattttct tatagaattg
    13201 attaattttt tatctgaaaa aatactactt tccccactaa ctcttatatc aagtatgtca
    13261 ttatatatat cttgattagt aatggaattt tttgtcagct tatcaataag agatccaaac
    13321 tcactgtcaa aagtgtcctc cttattaata caagctctga gctctttaac ataattattg
    13381 aaatccctat ttggttgaat agtagcctct attcctgtaa tatcagaaaa aatttcgcgc
    13441 gaggaactac ttctctcaga atagagattt tgatatgatt tattcattct tgacattgct
    13501 gagtcaacct caacttggta atctttaagc tttatttctt gttgaataat ttcttccaat
    13561 cgctcttcag tttttgataa agattgatat aaagaatcaa ggttttgtaa catttttaat
    13621 tcatcttgag ataactcttc actaagtttt tgatacctct ttgatacctc tttgaaacct
    13681 cattatagtt aactttccaa tctgatttat tcagctctgc tctataacta tcaatttcat
    13741 ctgacatttc attaattatt gtttgaatct tagcccgatt ggtcactaaa ttcttaatca
    13801 attctccgcg gtgttcatca atttcgtctg taacttttat accattgcca aagtcaattt
    13861 tgtcaacact atcagttaga tttttgggaa tttccttaag tgcctccaca tcacgtttca
    13921 gttccctata aatatctgtc tcttttgaat actcatcata aacatcttta taactcttat
    13981 gagatagctt ttctacttgt ttttttaaat cttcaatttc aagctctacc ttcgccttat
    14041 cttgtatttc ttttttcaca gaaacaatat cccaatttaa ttttcttatt tcgtttacac
    14101 atgtttcaat ctcattttta tactctgaaa tcaaatcaga gttatatcca tctagtaatt
    14161 ctagtatact agtttgattt tttgaaattt cataaatttg tttttgagag tatatactca
    14221 ccttatcgga aatcattctc aaccgttctg tttctttttc aaccagacca ttctcttgtg
    14281 taaattcata aatagaatgt ctatcctgtg ttcttattat ttgatatttt gtccccttat
    14341 agaaaatatt cagtttcaca attgtatcct tcaaaaatat tccagagcca ttatgttcaa
    14401 tttgtgcaaa gttctgataa tcactttgta tttcagaaaa ttgatcaagt tctgcttcct
    14461 ttccaaggac atagaccaag aatcttgtaa tacaagattt gcctgttcct cttcctccaa
    14521 taattgaagt taaattatcg ctgaattcga ctacaacttc ttttgtgcta agagtggtcc
    14581 cagaaaagct taatttttcc aataaaactt cattttcatt aatggagaaa ttagaaaaat
    14641 cagaatgaac tcgagtttcg cctagcatta aagcatcgcg aagtgatccc aagtcaggtt
    14701 cgtccttcat ttttatatag gaatatcttg tgcctattcc ccaaagtcca tgttttgaat
    14761 ttcctttcga gtgtgggtta tcagaaaagc taagaaatgt catattattt ttttctgcct
    14821 caacaactga tttataccaa gattccaaag tatcttttcc aactctacca tcatatctgg
    14881 cattcacact atcatagata gtgtcacgct tagatgaggg aatcatttgt gagcgatttt
    14941 caaaaaattc tttttgaacc gtctgtactg catatatatt ttctgattca aatatctttc
    15001 ctagatttcc aaattttgct gaagaaattc cattaaactc atctatatgt gcaggaataa
    15061 cgagcaagcc ctcgtgctca gcaaaatcaa taacttctaa aactgatttc attgagcaag
    15121 ctgcagcgct ggcccaatcc tctcttttta tacccatctt ggttaagaaa tcagagatat
    15181 catttgtaca tgcactttta tcaaatagga ctagtaaatg agttccattt tctccacacg
    15241 ttaattccac tccaggaaaa aaatctaagc cattttcgat agccactttc ttatattcat
    15301 caatattttc ccctgtattg tgatcagtta atgcaacaca atcgagtcct ttgtttaaac
    15361 attcttctac ccattcctta gcagtaacct ttttatcctg aaagcactga ctcgcagttg
    15421 aatgaatatg aagatcacat ttgtaccact tattgccaac cattagtaac acccctatca
    15481 acaaatattt tctatttcag tatatcaaat agaaaagtaa aagtacgata gaaatttctg
    15541 gtcaattcta acaaatgatt tacattagtt caaaaacccc tcgactcgta accaaataat
    15601 tcccgccaat ctccaagtct cgaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    15661 ctggtacttc gcctaaatca ccagcttcta caatcaagta atacgctaca tcactcatgt
    15721 cattacaatc gggataatag atgatatcat ccacatgctc taccatttcc tcgaaagaat
    15781 tgaagaaggc atgttgaatc tctgaagcga cctcaccaat tggcgttcct ttaagctctt
    15841 ccgctaaacc acacaaccga ttaatttcct caattggtgt atactcatca atctcaaaag
    15901 gcagttcata atcatgaatt gcgtactctt catattcgtc atttaatcca atccgttctt
    15961 tcacttcatc aaaatcgacg ggtggggtaa accatgcccc caccaactca ccttcattgt
    16021 attttccaag gttcgcaata taaacacgta tctgttccat tgtgattccc cctcaggcac
    16081 cgacaattcg gttaaacaaa ttgagtaaga catccttcac accagaagca ttaaagacca
    16141 gacctaccgc aatcaaagca atcaccaaga agccaatcag tttggaaaac tcgcgtttaa
    16201 atcctaaaaa caaaccaatg accacaatcg ctagtaagac taaagattg
//
LOCUS       DACCZY010000063        15715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCZY010000063 DACCZY010000000
VERSION     DACCZY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.063"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            72..1499
                     /locus_tag="IVZ67_001690"
     CDS             72..1499
                     /locus_tag="IVZ67_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8165768.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1591..1956)
                     /locus_tag="IVZ67_001691"
     CDS             complement(1591..1956)
                     /locus_tag="IVZ67_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8165769.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1953..2318)
                     /locus_tag="IVZ67_001692"
     CDS             complement(1953..2318)
                     /locus_tag="IVZ67_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8165770.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2510..2758)
                     /locus_tag="IVZ67_001693"
     CDS             complement(2510..2758)
                     /locus_tag="IVZ67_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165771.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2757..2885
                     /locus_tag="IVZ67_001694"
     CDS             2757..2885
                     /locus_tag="IVZ67_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8165772.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2942..4636
                     /locus_tag="IVZ67_001695"
     CDS             2942..4636
                     /locus_tag="IVZ67_001695"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8165773.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4736..4909
                     /locus_tag="IVZ67_001696"
     CDS             4736..4909
                     /locus_tag="IVZ67_001696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165774.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5287..6420
                     /locus_tag="IVZ67_001697"
     CDS             5287..6420
                     /locus_tag="IVZ67_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8165775.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6451..6771
                     /locus_tag="IVZ67_001698"
     CDS             6451..6771
                     /locus_tag="IVZ67_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165776.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6800..7264)
                     /locus_tag="IVZ67_001699"
     CDS             complement(6800..7264)
                     /locus_tag="IVZ67_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165777.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7396..8649)
                     /locus_tag="IVZ67_001700"
     CDS             complement(7396..8649)
                     /locus_tag="IVZ67_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8165778.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8678..8995)
                     /locus_tag="IVZ67_001701"
     CDS             complement(8678..8995)
                     /locus_tag="IVZ67_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8165779.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9021..9341)
                     /locus_tag="IVZ67_001702"
     CDS             complement(9021..9341)
                     /locus_tag="IVZ67_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165780.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9805..11220)
                     /locus_tag="IVZ67_001703"
     CDS             complement(9805..11220)
                     /locus_tag="IVZ67_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8165781.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11237..12553)
                     /locus_tag="IVZ67_001704"
     CDS             complement(11237..12553)
                     /locus_tag="IVZ67_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8165782.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12703..13401)
                     /locus_tag="IVZ67_001705"
     CDS             complement(12703..13401)
                     /locus_tag="IVZ67_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8165783.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13690..14679)
                     /locus_tag="IVZ67_001706"
     CDS             complement(13690..14679)
                     /locus_tag="IVZ67_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8165784.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="IVZ67_001707"
     CDS             complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="IVZ67_001707"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8165785.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4889 a         3186 c         2691 g         4949 t
ORIGIN      
        1 tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg
       61 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
      121 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      181 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      241 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      301 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      361 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      421 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      481 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      541 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      601 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      661 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      721 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      781 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      841 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      901 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      961 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
     1021 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1081 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1141 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1201 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1261 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1321 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1381 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1441 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1501 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1561 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1621 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1681 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1741 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1801 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1861 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1921 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1981 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     2041 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2101 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2161 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2221 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2281 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2341 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2401 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2461 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2521 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2581 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2641 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2701 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2761 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2821 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2881 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2941 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     3001 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3061 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3121 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3181 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3241 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3301 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3361 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3421 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3481 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3541 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3601 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3661 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3721 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3781 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3841 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3901 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3961 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     4021 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4081 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4141 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4201 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4261 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4321 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4381 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4441 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4501 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4561 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4621 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4681 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4741 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4801 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4861 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4921 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4981 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5041 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5101 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5161 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5221 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5281 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5341 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5401 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5461 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5521 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5581 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5641 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5701 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5761 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5821 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5881 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5941 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6001 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6061 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6121 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6181 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6241 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6301 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6361 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6421 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6481 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6541 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6601 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6661 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6721 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6781 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6841 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6901 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6961 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7021 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7081 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7141 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7201 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7261 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7321 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7381 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7441 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7501 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7561 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7621 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7681 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7741 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7801 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7861 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7921 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7981 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8041 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8101 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8161 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8221 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8281 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8341 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8401 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8461 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8521 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8581 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8641 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8701 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8761 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8821 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8881 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8941 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9001 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9061 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9121 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9181 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9241 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9301 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9361 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9421 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9481 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9541 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9601 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9661 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9721 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9781 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9841 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9901 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9961 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10021 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10081 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10141 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10201 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10261 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10321 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10381 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10441 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10501 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10561 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10621 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10681 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10741 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10801 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10861 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10921 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10981 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11041 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11101 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11161 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11221 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11281 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11341 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11401 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11461 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11521 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11581 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11641 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11701 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11761 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11821 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11881 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11941 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12001 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12061 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12121 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12181 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12241 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12301 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12361 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12421 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12481 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12541 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12601 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12661 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12721 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12781 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12841 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12901 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12961 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13021 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13081 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13141 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13201 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13261 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13321 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13381 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13441 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13501 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13561 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13621 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13681 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13741 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13801 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13861 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13921 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13981 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    14041 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    14101 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14161 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14221 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14281 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14341 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14401 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14461 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14521 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14581 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14641 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14701 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14761 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14821 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14881 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14941 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    15001 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    15061 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15121 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15181 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15241 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15301 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15361 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15421 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15481 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15541 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15601 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15661 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tcctt
//
LOCUS       DACCZY010000064        15420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCZY010000064 DACCZY010000000
VERSION     DACCZY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.064"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            147..2207
                     /locus_tag="IVZ67_001708"
     CDS             147..2207
                     /locus_tag="IVZ67_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165786.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2290..2859
                     /locus_tag="IVZ67_001709"
     CDS             2290..2859
                     /locus_tag="IVZ67_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8165787.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3034..3840)
                     /locus_tag="IVZ67_001710"
     CDS             complement(3034..3840)
                     /locus_tag="IVZ67_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165788.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3978..5492
                     /locus_tag="IVZ67_001711"
     CDS             3978..5492
                     /locus_tag="IVZ67_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8165789.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5895..6878)
                     /locus_tag="IVZ67_001712"
     CDS             complement(5895..6878)
                     /locus_tag="IVZ67_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8165790.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6883..7530)
                     /locus_tag="IVZ67_001713"
     CDS             complement(6883..7530)
                     /locus_tag="IVZ67_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8165791.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7615..8247)
                     /locus_tag="IVZ67_001714"
     CDS             complement(7615..8247)
                     /locus_tag="IVZ67_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8165792.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8263..8814)
                     /gene="coaC"
                     /locus_tag="IVZ67_001715"
     CDS             complement(8263..8814)
                     /gene="coaC"
                     /locus_tag="IVZ67_001715"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8165793.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8811..9548)
                     /locus_tag="IVZ67_001716"
     CDS             complement(8811..9548)
                     /locus_tag="IVZ67_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8165794.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9545..10534)
                     /locus_tag="IVZ67_001717"
     CDS             complement(9545..10534)
                     /locus_tag="IVZ67_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8165795.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10792..11334
                     /locus_tag="IVZ67_001718"
     CDS             10792..11334
                     /locus_tag="IVZ67_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8165796.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11351..12436
                     /locus_tag="IVZ67_001719"
     CDS             11351..12436
                     /locus_tag="IVZ67_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165797.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12448..13254
                     /locus_tag="IVZ67_001720"
     CDS             12448..13254
                     /locus_tag="IVZ67_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165798.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13255..14082
                     /locus_tag="IVZ67_001721"
     CDS             13255..14082
                     /locus_tag="IVZ67_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165799.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14079..15152
                     /locus_tag="IVZ67_001722"
     CDS             14079..15152
                     /locus_tag="IVZ67_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8165800.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4957 a         2787 c         3138 g         4538 t
ORIGIN      
        1 ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta atagtccatc
       61 ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact tcatgtttag
      121 acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa caactaaata
      181 aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac gttaagttta
      241 tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta cccgttcagg
      301 caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact gattccccta
      361 tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct gcattgactt
      421 cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt cctaacaaaa
      481 cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc gatatgactg
      541 atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat gctttgacag
      601 atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat tattcacagt
      661 ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct gatctggtag
      721 aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt tcaaacaatg
      781 ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc tattttgatc
      841 caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct gatcaaacag
      901 ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct aaagatcttg
      961 ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc aaaatgaacg
     1021 ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat atagactcga
     1081 aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct gctgcttcat
     1141 cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa gcagaaacac
     1201 atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt tctgatcgag
     1261 tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct atttctgatt
     1321 ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt gaagtacttc
     1381 actcagaact gatgaataat aggaagcaat caggagggga aattaccttt acttatgttt
     1441 atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa
     1501 taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc gtaccgttaa
     1561 aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg accaaacaaa
     1621 caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa gaactccatg
     1681 ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa
     1741 gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt tctccaacag
     1801 ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa atctcagaat
     1861 ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga ttggaaaatg
     1921 aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct aaacagtcgg
     1981 aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg tcaactattg
     2041 attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga acacccgcac
     2101 ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg gcaggaacat
     2161 gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt atatctctat
     2221 ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga gacgaggagg
     2281 gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac aagaccactt
     2341 caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa
     2401 gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca aacgacagat
     2461 gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact tgaaagaagg
     2521 atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa
     2581 aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaatc agattaccaa
     2641 ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc taccctccat
     2701 acggacggac caatctatct tcacacacag acatggagcc atcaggcgat tgctctttat
     2761 aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa tccagattat
     2821 gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga aataagtcct
     2881 cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt gagttaatac
     2941 tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga caaaagtatt
     3001 gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt taggaataaa
     3061 attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac caaatacact
     3121 gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca tgattcccac
     3181 gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat tttgactttc
     3241 aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt tacttaactc
     3301 cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca gtaatttatc
     3361 tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac agaaggaaac
     3421 aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga gcaaataaac
     3481 aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa aagtatccca
     3541 cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac taaaaataaa
     3601 agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct tttccagaaa
     3661 ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc cccagtgttg
     3721 gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa tatagattcc
     3781 gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca aatgattcat
     3841 aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt accaaagcta
     3901 tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata tagtataagg
     3961 agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc gttgcacttc
     4021 ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt tctagtacga
     4081 aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa
     4141 tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg gcagcagcta
     4201 tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct gttgccggag
     4261 gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt caaaaagaag
     4321 aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt ggtggaggaa
     4381 cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg gtcgattggc
     4441 tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg agtgaaaaga
     4501 ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag gggttattga
     4561 ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca gacattaaga
     4621 aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa ccaaaagagg
     4681 tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa gaatttattg
     4741 aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga tcaactggag
     4801 atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat aaaatacaaa
     4861 ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt cgcggagaag
     4921 gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat acaagggata
     4981 aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt tttgaccaag
     5041 gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat gtcacaactg
     5101 gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa
     5161 cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca
     5221 cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc gcaccaggaa
     5281 tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta gataaagaaa
     5341 accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta catggaaaca
     5401 accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa gcgggcatac
     5461 aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg
     5521 aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt agtgaagagg
     5581 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
     5641 gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc gtttattcgg
     5701 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     5761 cggcggaagc agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     5821 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     5881 tgatcagcat gattttattc agttatcttc aaaatgattt ttccgatgtt tttgtttgct
     5941 tccatatatt catgcgcttg tttgactgcg ctgaattgga atgtacggtc aatgattggc
     6001 cgaatcttgc cattttctag aaaaggccca gctttttgca taaaagtttg tgcgagctct
     6061 gctttatact gatcacttct aggtgtcaat aatgtaccaa gcagttggac acgtttccca
     6121 atcaatacac caaggtctat ctcaggcacg atggtcccac ctagcatacc aatcaagacc
     6181 caacgtccat caacggctat agaagctaaa ttcttttccc agtaagaagc gccgatgaaa
     6241 tccaaaataa catcgactcc ccgaccttca gtgatttttc gtacctcttc ttgaaagtct
     6301 tgttttttat agttgatcaa ttcatctgca ccaagttctt tacaaaaggc gagtttttct
     6361 tctgatcctg cagtaacgat gattttcgcg tgagaaagct gtctagctaa ttggatcgct
     6421 gctgttccca caccacttgc tcctgcatgg atcaagacag tttcgccttc ttgtaatttt
     6481 ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc agcagcagaa
     6541 acatagtcta gcgtatcagg gagaaggatt gcacgatcgg caggcatcac agcatattcg
     6601 gcataaccac ctagattgac taatccgtat acttttgtcc caggagtaaa agtggagata
     6661 tcacttttat tctcaactac ctctccagct acttcgactc ccaaaatagg gtagggaggt
     6721 ttcaaagaga ctgattctct tttcataata tctgttcgat tgacagctgc tgtatggact
     6781 ttgatcagta attcgccttg ttttggttta ggaacaggca cttcaatttg ttctaataca
     6841 tctacagaac caggatgctt gattgaaatg gcacgcatgg atctattcct cctttgccca
     6901 ttcttggaat gctgtattca actctttgct accttcagaa agtactagag tcgtacgaga
     6961 aatatccgtt tcttccacta gagcggcaag tacagcagca actgtagagc gagtgatcgt
     7021 ttttgatgcc ttctcattag gcttagttaa ttgaatggaa gtaacttctt catcgttagt
     7081 caatgtgact ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc attcatctgc
     7141 ataatgttta gtaatataat aatctgtcat ggactcaggc cattttgctg gttcatctgc
     7201 ataaacagca ctgaccatca gatagcgaga gacatgcgca ttttcagctg cgatcactgt
     7261 tttgattgcg ccatccaagt ctacttgcag aagattcttt ccttgagagc cggcagcaaa
     7321 aatcaataca tcgatgcctt tgaatgcttc ggtcattttt tccacggacc aagttaaatc
     7381 aaaagagaca tacgaaacat tctctttttt tactaatgtt tgggaagcaa catctctcac
     7441 ccctgcaaaa atttcatggg aacttggagc taaatctttt atcaggtgtc ggccaatttg
     7501 tccgtttgca cctacaacaa aaattttcat ttggatcatc ctttcgacgt caaatctttc
     7561 cattcaatag taacaccaaa agagaaaaaa atcagagatc ggctacgatg actatcagtt
     7621 agtaagagaa gggtgcttga agtatcttaa caagacattg catactgctg cagtcgcaag
     7681 acctgcgacg attccttcag gtataccact tgtaaaaata accgctagaa tagctgaata
     7741 aacagcattt aaatttgttc cgatgacttt ggcataatct tgctggaata aaaagtagat
     7801 catattcatc actaaaatcg tattggtcaa tgaaccggaa agtccagcta agaataaaga
     7861 aacggaatct gctttttgtt tcattagttt gcgcagacct ttataaacga aataaggaac
     7921 caccccaatc aagatgcgag gaataaacgc gatcagcaag gctttccagc ttccatgttc
     7981 tgttccgatg actgggataa acggagaaaa gacaaaagac aaaggtgttt ggataactgt
     8041 gctacggatc atgctgatca gtccaaatgt accgccaaga aaagcaccta gtcttggtcc
     8101 taaaacgatc gaagcaatga ttacaggaat atgcatggtt gttgcattga ttggaccgat
     8161 agggataaaa cctaaaggcg taacagctaa taaaatcaaa atagctaaaa acatcgcagt
     8221 taatgtaaag ttttttgtat ttttcataag tactcctttt ttctaagcat ttctttcatt
     8281 aagagtcagc atgacctgtt cgatgatcgt ctgaatatca gctaaggcac cttttccaaa
     8341 atctccacaa gctaaaagag attctcttgg ttcaatttcc tgatagccga cagatctcaa
     8401 tgtttccaga ttacgctgag tgatcgggtt ttgatacata ttcgtgttca tggcaggggc
     8461 aatcaatttt ggtgttccca ctggaagcgc cattgcgact gttgaaagaa gatcgtctgc
     8521 aatgccgttt gctagcttgc cgataatgtt tgctgtagca ggcgcgatga gaaacaagtc
     8581 agcttgtttt gctagttcga tatgattgat tacctgcgga tttgattctt gcatgacgtc
     8641 tgtatggact gcgcgcttcg acagagattg caaggttaat ggcgtgataa aatgtgtgct
     8701 gcttttagac ataatcacat ctacctgggc acctaatttg acgaattgac ttgtaagatc
     8761 agcgcttttg taagcagaaa tacttccagt aacgcctagt aagatctttt tcattttgat
     8821 tcctccagtc taattttctc agtaatcaat gcagcaatct cagatttact ttgagcttct
     8881 tccactgttc catctttaga taagagatag ccgtggtgtg tctcgccatg gatttccgtc
     8941 aaatcatttg cgaaaataaa atcagttttg ttatttatca gactattttt tgcgactgcc
     9001 agaagagatt cctttggtac atctactaat agtttgaaac caatgatttt tgcttttggc
     9061 tgccattcac gtagataaga aatgattttt ggtgttttct tcaaaacgag cacaagatgc
     9121 tcggtgttag aagagatctt tgaaacagtg gtatctgtct gttcattttc cgcaaaccaa
     9181 ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg ttcttctgaa
     9241 aaactaaaag caggagtaaa atcactgact gccatactgt ggataatcgc atcgtagtgt
     9301 tcttttttcg ttagttcttc taattgtaaa aataagtcca gtgcagattc gatcgtataa
     9361 atcgttatat tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac gtaatcgatt
     9421 gtggtatccg gattggcagc taaataatca gctaatgctt gccctaatcg tcctgtcgaa
     9481 tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc tgtgattaga
     9541 atcttcattt atggtcacct acatttacta gtgttcttcc ttggtgtgtt ccagcaagta
     9601 gtttagatgc tgtttcaggc aattgatcca gagtaatctc ttggacgacc agttcatctg
     9661 caatctgcaa atccgccatc tgttgccata aagaaagacg ttgatctatt ggtacattga
     9721 cagaatcaat tccaatcaac tggatgtttc gcagaataaa aggcaagaca gtcgcgttta
     9781 gtttgattcc cccagcattg ccacataaag ctactgcacc gttataagag atcaatggaa
     9841 gaagactgct tagctgctcg ccacctactg tgtcgatgac atagtcgatt tgctgttttc
     9901 ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg tacgacttgc aaagcaccta
     9961 gactttttag ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt gactggaatc
    10021 ctagtttatg taacagggct atcgctgtac tgcctacacc gccagaagca ccagtaacga
    10081 caactttttt atcggaggtc ataccttgac ggattaaagc gttcacacac aacatagcag
    10141 taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt catatttttt ggtagaggaa
    10201 ctaaccagtc tcctggtact ttttgatatt ggctataacc gcctggatgg gtgacgccaa
    10261 gcccaaaacc agtagcaagt acaagatctt cttttgaaaa acgtggattg ttactttgga
    10321 cgacagttcc ggataaatca atccctggag tcatcggata tgttcgtatc acctgaccag
    10381 actcgctcat tgccaatgca tctttataat tgacatcaga ataggcaatt tttaccaaga
    10441 cttcattttc agccaactca gtaaatggca gatcactgat tgctgtatgg aaaacaggtt
    10501 cctttattac ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt ttaaaatttt
    10561 cttttatcat accataaagc ctactctttt attcttgttc ttccgtaaat ttagacaaaa
    10621 attaggtgaa aaataaagaa tcaaacaact agaatttatt ccaaaagata aagaaatatt
    10681 cagcaaaaaa aattttacaa agggttgcct ttttcaataa atttcgtata ctaatacgag
    10741 tggtagtaaa caggaagttt actattagaa aacttcgatt cggaggaaat tatggaaatt
    10801 ggtgagaagt taagaaattt gcgcatccaa aagaatttga cacaagaaga actaggcgaa
    10861 cgcacagatt tgtcaaaagg gtatatttcc cagttggaac gagatttaag ttcaccatca
    10921 atggaaacat tcttttctat tcttgaagtc ttaggtgtga ctccagaaga attttttcat
    10981 caagaagcag cgaatctgca agtggtttat tcaaaagaag atcatacggt ttatttcgat
    11041 gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa agaaatggag
    11101 cctgtcttgc tgacttttga aggtgcagga gaatataaga cgtttgaacc ttcattatct
    11161 gaaacgttta tttttgtatt agaaggggaa ttagagttaa gcttaggaga gacggtctat
    11221 gtggcaaaag caggacagtc gatctattat caagcgacaa aacaacatca attaaggaat
    11281 catctcaaaa aacgaacaaa agtattgatt gttgcaacgc aatcatattt gtaatatagg
    11341 gggtaaaaac gtggggaaac caatcatttc atttgatcaa gtagtgaaac gctatgatga
    11401 tgaaacagta ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt atacactgct
    11461 tggtccttcc ggctgtggta aaacaacgat cctacgtatt atagctggat tcaccgaagc
    11521 tacagaaggg gacatctatt ttgaagggaa acggttgaat gatttgccag ctaataagcg
    11581 acaagtaaat acagtcttcc aagattatgc actttttcct catatgaatg tttttgataa
    11641 cgttgctttt ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa agaaggtaaa
    11701 agatgctttg cgtatggtcc agcttcctgg atacgaaaaa cgggagatta gtgaaatgtc
    11761 tggtggtcaa agacagcgtg tagcgattgc acgagcaatt gtaaatgaac cgaaagtatt
    11821 gctattagat gaaccgttat cggcgcttga tttgaaacta cgtacagata tgcagtatga
    11881 actgcgggat ctgcaacaac gattaggaat cacgtttatt tttgttactc atgaccaaga
    11941 agaagcgttg gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa tcgtccaaag
    12001 tggtacaccc gtagatattt atgatgagcc aatcaatcat ttcgttgctg attttgttgg
    12061 ggagagcaat atcgtggacg gtgtaatgat cgaagataat ctagttgagt ttgttggtaa
    12121 gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag ttgttttacg
    12181 cccagaggat ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa cggtcgatac
    12241 tcagctgttt cgtggtgtcc attatgaaat catctgttat gatgaacagc aaaatgaatg
    12301 gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg cctttgagcc
    12361 agaagatatc catgtcatgc gtttcaacga gtctgaagaa gaattcgatg cacgtcttga
    12421 cagctacgaa gaataggggg gacaagaatg aaaacaatgc gcaggatcta ttccgtccct
    12481 tatattttgt ggctcggttt attcgtgatc gcgcctgtat taatgatcat ttatcaatct
    12541 ttttttgata tgaatgggca attcacgctg aataattatc tatcttattt tacgtcgggg
    12601 acatcgcttt ctatgacgat caattctgta tggtatgcat ttttgatcac gtttttcacg
    12661 ctgatcatca gttacccgac tgcttatttt ttaacaaaac tgaagcacaa gcaattatgg
    12721 ctgatgctga ttattttgcc tacatgggtg aatctgctgc ttaaagctta cgcatttatt
    12781 gggattttca gtgtgcacgg cagcgtgaat catttctttg attttatcgg gatcgggccg
    12841 aaacagatat tattcacaga cttcagtttt ctgttcgtgg caacgtatat cgagattcca
    12901 tttatgatta tgccaatttt caatgcattg gaagagatga atccttcttt gatcagtgct
    12961 agtagagatt taggcgcgaa tagctttgag accttccgac gagtgatttt tccgttatca
    13021 ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc cttctctttc tttattcatg
    13081 ctgactcgtc tgattggcgg aaatcgggtg atcactttag gaacggctat cgaacagcat
    13141 ttcttggtta cacaaaactg ggggatgggt tctacaatcg gtgtgatttt aatcattgct
    13201 atgttcatcg tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc ataaatgaaa
    13261 aaatttaaat ggtcgtatct ttatttaggg atcgtctttt tattcctata tgcaccgatc
    13321 ttctatctga ttttttattc cttcaatgaa ggaggaacga tgaacagctt tacaggattt
    13381 acttgggaaa attatgaagc tgtttttgaa gatacacgtt tgatcattat cgtattgaat
    13441 acgttcatgc tggctttctt atctgcattg atcgccacga tcatcgggac atttggggct
    13501 atgggaatct attacacgaa aaaacgacgt gcacggacag cgttgttgag catgaataac
    13561 atcttattag tctctcctga tgttattatc ggtgctagtt tcttgatttt ctttacacta
    13621 ctaggattcg gattaggttt tcagagtgta ttgctttccc atattgcttt cagcattcca
    13681 atcgtcgtct tgatggtctt gccaaaattg caggaaatga acgattcaat ggtcgatgcg
    13741 gctagagacc ttggtgctaa taatttccaa gtagtcaaaa atatcatttt gccttatctt
    13801 tctccaggaa tcatagctgg atattttatg gcatttacct attctcttga tgattttgcg
    13861 gttacatttt tcgtcacagg aaatggcttt acgacgctct cagtagagat ctattctcgt
    13921 gcaagacaag gaatcagttt agaaatcaat gcgctaagtg cgttagtttt cttgttctcc
    13981 atgatattgg tcattggata ctactttatt agtaaagaaa accgtccaaa acggctgaag
    14041 aaaaacagaa gagtacgcga gggggtggca cggctgcgat gaaaaaatta caatctctgt
    14101 ttttaggtat tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa cttgaaaaaa
    14161 caagtggcat gagtggagca aaggtactta ccatctataa ctggggagat tatatcgatc
    14221 ctagtttgat cacgaaattt gaaaaagaat atggatacaa agtgaattat gaaacatttg
    14281 attctaatga agcgatgttc acaaaaatcc agcaaggtgg gacaaactac gacatcgcta
    14341 taccaagtga atatatgatc caaaaaatga tcaaagaaaa actagtactt ccattagatc
    14401 attcgaagat caaaggatta aataatattg atccacgatt tttagacttg gattttgatc
    14461 cagataatca gtattcgatt ccatattttt ggggaacttt agggatcgta tataacgata
    14521 aattctttga tgcaaatcaa attaagcatt gggatgattt gtggaagccg gaattaaaag
    14581 acagtttgat gctgattgac ggtgcacgag aagttatggg gctttctttg aatagtttag
    14641 gctattcttt gaatagtaag aatatggcac agctgacaga agcagcaaat aaattgaatc
    14701 atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat atgatcaatg
    14761 aagaagcctc cgtggcagtg acattttctg gcgaagctgc cgatatgatg tgggaaaatg
    14821 aacatttaca ttatgtgatc ccaccagaag gatccaatct ttggtttgat aatatcgtta
    14881 tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt catcaacttt atgctagaac
    14941 cggaaaatgc ggcacaaaat gcagagtata ttggttattc tacaccaaat gaagaagcaa
    15001 aagcgttatt gccaaaagac atttcgtcgg acgaacaatt ctatccatct gatgacacga
    15061 tctcccatct tgaagtctac aaagatctag gatcgaaata cctaggcatc tataatgacc
    15121 tattcttaga attcaagatg tataggagat aatggcaaaa ctcagtaaga atggggactt
    15181 atcaacgttc taaagagcta aaaatcgtcc tttttctatc tgagttttcc aatcttctac
    15241 caaaagcgga tgaaacgccg ctttttttgt gttattttat tttttctacc acgtttttct
    15301 accagagaaa agtggacaaa accattgtaa aatagggccc gagacgggct agtaaatcta
    15361 agattgaaaa agttacagaa ggataaagta attggaaact atgcaaatat acttaaccct
//
LOCUS       DACCZY010000065        15406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCZY010000065 DACCZY010000000
VERSION     DACCZY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.065"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(29..406)
                     /locus_tag="IVZ67_001723"
     CDS             complement(29..406)
                     /locus_tag="IVZ67_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165801.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(706..1587)
                     /gene="galU"
                     /locus_tag="IVZ67_001724"
     CDS             complement(706..1587)
                     /gene="galU"
                     /locus_tag="IVZ67_001724"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8165802.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1630..2649)
                     /locus_tag="IVZ67_001725"
     CDS             complement(1630..2649)
                     /locus_tag="IVZ67_001725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8165803.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3282..4286)
                     /locus_tag="IVZ67_001726"
     CDS             complement(3282..4286)
                     /locus_tag="IVZ67_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8165804.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4299..5348)
                     /locus_tag="IVZ67_001727"
     CDS             complement(4299..5348)
                     /locus_tag="IVZ67_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8165805.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5365..6186)
                     /locus_tag="IVZ67_001728"
     CDS             complement(5365..6186)
                     /locus_tag="IVZ67_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8165806.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6173..6952)
                     /locus_tag="IVZ67_001729"
     CDS             complement(6173..6952)
                     /locus_tag="IVZ67_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8165807.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6969..7439)
                     /locus_tag="IVZ67_001730"
     CDS             complement(6969..7439)
                     /locus_tag="IVZ67_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8165808.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7451..7870)
                     /locus_tag="IVZ67_001731"
     CDS             complement(7451..7870)
                     /locus_tag="IVZ67_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8165809.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7951..10719)
                     /locus_tag="IVZ67_001732"
     CDS             complement(7951..10719)
                     /locus_tag="IVZ67_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8165810.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10919..11143
                     /locus_tag="IVZ67_001733"
     CDS             10919..11143
                     /locus_tag="IVZ67_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165811.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11922..13637
                     /locus_tag="IVZ67_001734"
     CDS             11922..13637
                     /locus_tag="IVZ67_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165812.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13640..15403
                     /locus_tag="IVZ67_001735"
     CDS             13640..15403
                     /locus_tag="IVZ67_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165813.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4496 a         3188 c         2635 g         5087 t
ORIGIN      
        1 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
       61 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
      121 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
      181 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
      241 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
      301 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
      361 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
      421 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
      481 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
      541 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
      601 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
      661 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
      721 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
      781 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
      841 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
      901 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
      961 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
     1021 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
     1081 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
     1141 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
     1201 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
     1261 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
     1321 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
     1381 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
     1441 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
     1501 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
     1561 aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga ttaactattg
     1621 atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta acatgatttc
     1681 ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg tttgagtaat
     1741 tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg ttgccacacc
     1801 ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct gtcctaacaa
     1861 gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca agtcccccac
     1921 accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa gacggctgat
     1981 ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc ctaatccatg
     2041 gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt ctacgccaat
     2101 cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt gtacataggc
     2161 tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta ctgccacttc
     2221 ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt ctggaatttc
     2281 ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt tacttgcatg
     2341 tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg aacgaatcgc
     2401 ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg ccaattcttt
     2461 atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat ttgtatgata
     2521 cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat catgcccgtt
     2581 ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa ctgcgatttt
     2641 ttgtttcatg gatattcctc caaataaata attgataatt tccattatag tatacatttc
     2701 ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt tatcgttttt
     2761 acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca ctcgttatgc
     2821 tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt actattagga
     2881 ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca gattgaacat
     2941 ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt gacataggaa
     3001 cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc aactcgttgt
     3061 acttggactt ttactacgga tccatgcaac aactaaatag tactattgca taacaaaaaa
     3121 tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga taatgcaaaa
     3181 aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga gcagttatcg
     3241 ctgctctctt tattttttat attcttttta tccagtctat cctaataatc aaactgacga
     3301 tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc atcgatgcgt
     3361 tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt ttcactgata
     3421 ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat ttttggcaaa
     3481 agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa taatgtgact
     3541 ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc atggatagat
     3601 ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata aacagctccc
     3661 cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt ttctgcaaat
     3721 gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg taaataacgt
     3781 tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata catcaatcga
     3841 tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt gaccacaaaa
     3901 tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag tgtcgctcct
     3961 actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt aacagaggac
     4021 aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata ttctgcagca
     4081 tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc ttgcatcgag
     4141 gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc cgcataaagt
     4201 ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat tgctttgatt
     4261 tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact tttcaagact
     4321 gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac tgcggtttta
     4381 taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc atcacattct
     4441 attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt gagagtctct
     4501 ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt tacactttcc
     4561 ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc gtgcatataa
     4621 gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt cgtctctgct
     4681 tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg tgctaaacga
     4741 aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc tggtaaatgg
     4801 ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct gattttattt
     4861 ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc agaatagcct
     4921 tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg tgcagcttct
     4981 aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc ttgtttttgt
     5041 atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac agcaatcaca
     5101 gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc ttgaatatcg
     5161 acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc gttataagat
     5221 gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct tgtcgaaaaa
     5281 tcattctttt tcaagatcgt acttagtact tccttttctt catagatata atcttgcatc
     5341 gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa aaattccaaa
     5401 gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata gataaaacac
     5461 tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa tactttgaac
     5521 agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga tcatcgtggc
     5581 agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca aactttccat
     5641 cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc cccaacctgt
     5701 tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca agatagggcc
     5761 taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca atcgcaaagt
     5821 tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg ttttgatact
     5881 atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca ttgcagccgt
     5941 gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta gatgccgttt
     6001 caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg gaatcattgc
     6061 ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca tttgaaaaga
     6121 acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga tctcagaatt
     6181 cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg acattcataa
     6241 tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc acaggaatct
     6301 ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt ttgttgatca
     6361 gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct actgacaacc
     6421 ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg cttcccacca
     6481 tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg gcaagcaagt
     6541 gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct ttatggttca
     6601 taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac gtagcaatcg
     6661 gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca aaagccgtcc
     6721 ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag aacgatccga
     6781 taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca cctaacacga
     6841 ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct agtgcatatt
     6901 ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc attgaggttc
     6961 ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac ttgacggatt
     7021 tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc tggtaaaata
     7081 tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc taagttgatg
     7141 gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga ttccactacg
     7201 atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga gtcatcaatt
     7261 gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt ctttcgtatc
     7321 tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat tgtttgtgtc
     7381 catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa tacaatcatt
     7441 tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg ttacaaaact
     7501 ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc tctacttcag
     7561 cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca acgagataga
     7621 catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca aaaagatccg
     7681 tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact gtttccgcta
     7741 aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt ttcccataga
     7801 tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct aagataattt
     7861 ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc aagttgcatg
     7921 ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg agagaaactc
     7981 tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga tcatgacact
     8041 ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt ctttttgaca
     8101 ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga tcaatcgttc
     8161 aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga tgcttcgatt
     8221 caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct tctctgtatc
     8281 tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga tgcgctgatt
     8341 gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta gtacagacat
     8401 cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga tatcaggatc
     8461 tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac tgtttttctt
     8521 atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg taggtaagct
     8581 tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc aagtagtaat
     8641 gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct ggatctgtcg
     8701 aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt cccctactgt
     8761 gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata actgtttttt
     8821 aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca atccatttga
     8881 aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg gtgtgacatc
     8941 tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta ctacatttgc
     9001 catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca gtcgtttttc
     9061 ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca tacttgtgtc
     9121 ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt gataactaga
     9181 tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa tatcatgttc
     9241 ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg catagacact
     9301 gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct ctcggataga
     9361 ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa acagcaaaaa
     9421 atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata gcagacagtt
     9481 atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct gctgttcggg
     9541 ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga ttccttgctg
     9601 tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat ggatcgtgac
     9661 atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa ctttgacgat
     9721 atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg acaaagctgg
     9781 tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa aagagtaaat
     9841 cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga ttcggatagg
     9901 tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg tagcaaaaaa
     9961 gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa tcacaccttg
    10021 atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta cctcgtctaa
    10081 aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt ctgcaccagt
    10141 gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat tatctgcata
    10201 ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat gaagcagaca
    10261 atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc ctgtaatcaa
    10321 aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga tactttcttt
    10381 caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg cgaaaaaagg
    10441 ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact taaaaaattg
    10501 tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt tgactagtag
    10561 ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta ccccaaaatg
    10621 atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga aaaaggctgt
    10681 ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc ctcctccatc
    10741 atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa aatatatctt
    10801 attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg tacagttcct
    10861 tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg actattcaat
    10921 gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc gagaaatcat
    10981 cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta tgacagaaca
    11041 aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt tattcatctt
    11101 tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac agatttcaac
    11161 tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac aatttattgg
    11221 cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact gttgtcacca
    11281 tcctttttat tgattacgta caccactctt agttttttcc cattgattac gaaaacaagg
    11341 tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa aatctcaagt
    11401 ggcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata gaatctgcgc
    11461 caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac aataaacgaa
    11521 taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt ctctcaaccc
    11581 acttttttac aaaaattacg tagcaaaaaa gattgcaaga acaaattaaa aaagaagtaa
    11641 atagagtttc atctataaac atttacttga tatatttttt atgcagaatt catttataga
    11701 aaaattaagt aaaatagacg ttcctatgaa tgaagagact ggcatgtttt ctcattttct
    11761 tttttttaaa aaaaatagtc tattcaattt ctaaagcttc ttcataataa aactttcatt
    11821 tatgaaaagg cttgttctct tttcattcta aatttcctaa gctacaatgt ggtagtactt
    11881 gttttttcaa ttgtggacaa ataaataata ggaggaatca tatgattcag ttattacgtt
    11941 atgcaaaaga ctatcgtaag caaatcattt tgggcccgtt cttcaaattt cttgaagctg
    12001 tcttcgaact tgtcctgcct ttaatgatgg cttccttgat cgataatgga ctaaaaatga
    12061 atgaccgagg aaaaatcatt gaaatgggct tatggatggt cgctatgtct gtgattggtc
    12121 tgatttgtgc tatcatttgc caatactatg cttcagtagc ttcacaagga tttggtacag
    12181 agttacgtaa tcagctaatc aaaaaaatca atactttctc tcacaacgag ttgaatcatt
    12241 ttggaacaga taccttgatc acccgaatga caaacgacat caatcagctt cagctagctt
    12301 tagcaatggt cattcgttta ttgatcagag caccgttttt aagtatcggg tcagtggtca
    12361 tggcttttgt gatcgactgg gaagtaggtc tgtttttctt agcattattg cctattttct
    12421 caatcatttt attttttatc atcaaaaaaa ccgttcctct ctaccaaaaa gtgcaagaaa
    12481 aactagatca gctcaatgaa cacgttagtc agaatcttag cggtgttcgt gtcatccgcg
    12541 catttgcgca gacaaaaaaa gaaacagaga agttcgataa aagcacaaat gatctagcga
    12601 acaattattt acgtgtttcc aatatctctg ccctattatc acctgctaca accttgatca
    12661 tgaatgtcgg gatcatctgt ttgctgacag ttggcgggat caaagtaaat attggtagct
    12721 tgcaacaagg acaagttctt gctttgatca attatatgaa tcaaatgctt ttggcactga
    12781 tcgttgtttc aaacctcgtc gtgatcttca cacgtgccga agcttcagga aatcgggtga
    12841 aagaggtgct agacacagaa aattcgattc ttgaagctga ggtcagtact actcccgatt
    12901 tttcttctga agcaattatc gcttttgacc atgttgactt ccgttatact cctgattctg
    12961 gtctttctct ccaaaatatt acatttcagc tgaagcgaaa tacagtcctt ggaatcattg
    13021 gtccaaccgg aagcgggaaa acgactttga cacaattgat tccgcgtttc tatgatgtca
    13081 gttccggtac agttataatg gacggaatgg atgttcgttc ttggcagctg gatcatctcc
    13141 gtcagcaaat tgctcttgtt ccccaaactt ctgtactatt taccgggact attcgtgaaa
    13201 accttcaatg gggtaagaaa gacgccacag aaaaagaatg ttgggaagct ctgaaaatcg
    13261 ctcaggcaga agaatttgtc cgtcagcttc caaatggttt agatacaaaa gtcatggaaa
    13321 atgggaaaaa tttctctggc ggacaaaaac agcgactgac gattgcaaga gctttgattg
    13381 ccaagccagc attactgatt ttagatgact ctttaagtgc gttggattat caaacagact
    13441 tggatttgcg taaggcgcta cagacttatc ttggcagtac agtgattatt atctcccaac
    13501 gtgtccgctc tattcaagaa gcacatcata tcttagtgat ggatcaaggt aagattgctg
    13561 ctcagggtac ccatgaagaa ttacttgctc aatcagcaga atatcaggaa atcgtcgcat
    13621 cacaggagga agaatagtta tgaaagtaaa aaaattatcc ttgcagacgc tccgccgatt
    13681 cattccttac cttactgcct atgcaaaaga catttggcta gctgtgatac taggaatcat
    13741 caatgggatt gcaacagttg tgatgacgtt acaaattggt caaagtatcg atcaaatgat
    13801 tggagaagga caggtccgct tcaatcgact gttccattta cttattctat tcggtggtat
    13861 cgttttaatc aacgtagtca gtcaatggct catccaagtg ttaagcaatc ggttagctta
    13921 cttatccgtt gcaaaattaa gaaaagatgc atttgctcat ctgaatcgat tgccacttcg
    13981 ttattatgat caaaactccc acggaaatat cgtcagtcga tttacaaatg atatggacaa
    14041 tatttccatt gcaatttctg cggccttcaa tcaattattt tctggagtgg caatcatcat
    14101 tctttcgttt atttttatga tgcgtctaag tccacttctg acattggtcg ttctttgttc
    14161 tacaccaatt attttccttg tgagttggat cgttgctcgt gcttcacaaa caaatttcga
    14221 tagacagcaa aggattattg gggatatctc gggctttgtg accgaacgaa tcggtaacca
    14281 gaaaatcgtg aaagcttttc agcaagaaga agctagtcaa cagcaatttg aactgctaaa
    14341 tgctgatcta tatgaaaaag gacaacgggc acaattttct tcttccttga caaacccgtc
    14401 ttctcgtttt gtagatcacc ttgcttattt atcgattggt ttagttggcg gcctattggc
    14461 attgaagagt ggttcgtcaa tcactgttgg tgtgatttcc agctttacga tctactcaag
    14521 tcagttttct aaaccattta tcgaactttc tggaatcacg acacaactgc aaaccgcttt
    14581 ttctggtcta gaacgagcat ttgatttgat cgatcagcca gaagaaaagc ctgatttgcc
    14641 agatgcctat gccttggaac ccaaaaatat caaaggaaaa gtctcttttg atcatgtttc
    14701 tttttcttat gaccccaagc agcgtttgat cgaagatttc aattttactg cttatccagg
    14761 tcaaaccatt gctatagtag gaaaaacagg ggccggaaaa tccacacttg ttaatttgct
    14821 gatgcgtttt tatgacacaa caaaaggaac aattacagtc gatgaccatg acatccgctc
    14881 tatccaacgt gatacattac gtaaaagttt tggaatggtg ttacaagata cttggctatt
    14941 tgacagtaca ttgcgagaaa atcttcgttt tgggaatccg gaagcaagtg acgcagaaat
    15001 caatgatgcc ctaaaagcct cttatatgta tgaatttgta gaacgtttgc cggaaaaatt
    15061 agatactaag attggcggtc aagggttaaa gatatcagat ggacaacgcc aactgttgac
    15121 gattgctcga acgatgatca gtaatccccc tatgttgatc ttagatgaag ctaccagctc
    15181 agtcgataca ttgacagaga aaaaaatcca acaagccttc ttagcaatga tggaaggaaa
    15241 aaccagtttt gtgattgccc atcgcctgtc cacaatcaaa aatgcggacc agatattagt
    15301 gatggatcag ggagctattg tcgaaatggg cacccatgat gagctgcttc aaaaagatgg
    15361 ttattaccga cggctctatc aagcacaatt cacagcacaa taaaaa
//
LOCUS       DACCZY010000066        15171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCZY010000066 DACCZY010000000
VERSION     DACCZY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.066"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(129..371)
                     /gene="acpP"
                     /locus_tag="IVZ67_001736"
     CDS             complement(129..371)
                     /gene="acpP"
                     /locus_tag="IVZ67_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8165814.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(493..1494)
                     /gene="plsX"
                     /locus_tag="IVZ67_001737"
     CDS             complement(493..1494)
                     /gene="plsX"
                     /locus_tag="IVZ67_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8165815.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1543..3579)
                     /gene="recG"
                     /locus_tag="IVZ67_001738"
     CDS             complement(1543..3579)
                     /gene="recG"
                     /locus_tag="IVZ67_001738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8165816.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3696..5378)
                     /locus_tag="IVZ67_001739"
     CDS             complement(3696..5378)
                     /locus_tag="IVZ67_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8165817.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5430..5792)
                     /locus_tag="IVZ67_001740"
     CDS             complement(5430..5792)
                     /locus_tag="IVZ67_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8165818.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6062..6250
                     /locus_tag="IVZ67_001741"
     CDS             6062..6250
                     /locus_tag="IVZ67_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8165819.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6459..6863
                     /locus_tag="IVZ67_001742"
     CDS             6459..6863
                     /locus_tag="IVZ67_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8165820.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            6880..8028
                     /gene="tnpB"
                     /locus_tag="IVZ67_001743"
     CDS             6880..8028
                     /gene="tnpB"
                     /locus_tag="IVZ67_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8165821.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            8194..8805
                     /locus_tag="IVZ67_001744"
                     /pseudo
     CDS             8194..8805
                     /locus_tag="IVZ67_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            8802..9575
                     /locus_tag="IVZ67_001745"
     CDS             8802..9575
                     /locus_tag="IVZ67_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8165822.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            9572..9970
                     /locus_tag="IVZ67_001746"
     CDS             9572..9970
                     /locus_tag="IVZ67_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8165823.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            10155..10826
                     /locus_tag="IVZ67_001747"
     CDS             10155..10826
                     /locus_tag="IVZ67_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165824.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            10885..11325
                     /locus_tag="IVZ67_001748"
     CDS             10885..11325
                     /locus_tag="IVZ67_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165825.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            11337..12005
                     /locus_tag="IVZ67_001749"
     CDS             11337..12005
                     /locus_tag="IVZ67_001749"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8165826.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            12069..12692
                     /locus_tag="IVZ67_001750"
     CDS             12069..12692
                     /locus_tag="IVZ67_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8165827.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            12748..13134
                     /locus_tag="IVZ67_001751"
     CDS             12748..13134
                     /locus_tag="IVZ67_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8165828.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            13347..14738
                     /locus_tag="IVZ67_001752"
     CDS             13347..14738
                     /locus_tag="IVZ67_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8165829.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         4577 a         2877 c         2981 g         4736 t
ORIGIN      
        1 ctttgcaaca taatttctgt ttatcgccag aattatcaga aaaatgactt ttacgtgtca
       61 aaaagattaa aaaaagaagg ataaaaagct gtatgcagct ttttatcctt ctttgctttt
      121 atttgaaatt atttaagatg gctgctgata taatctactg cgccaccgac tgtttcaatt
      181 tgttcagcgt cttcatctga aatttccgta ccgaattcat cttcaagctc taaaacgaat
      241 tccataatac taattgaatc agcttttaaa tcatctttga tgttcaattc atccgtcact
      301 ttatcggtat ctatctcaaa atgatttgaa ataattttcg ctactttgtt aaatacttct
      361 tcgcgagtca actttttcac ctccaagaat catacacttg tcaattacct acaccatggc
      421 taattttact ggcatttttc aggaatgtcc accttttttt caatggaacg gattgcttca
      481 gatatccaaa tactattctt cgcttttttc aaattgttcg actaattgtc caactacatc
      541 tgtctcaagc attgtgtgga tttggcggat cgtatagcga acagcatcgg ggcctgttgc
      601 tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa acggcaccgc catgtttgga
      661 ataatccatt tctgctttca aagaacgtaa gccgtctttt aaaagcatag cgcccatttt
      721 tcctttgatc ccttcattta gaatagcaga ttttaataga ttcatcatat tcattgctgt
      781 tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc
      841 cacaccattg agtagctcac gagcttctac gtttccaata aaattcaagc ttgtttcatt
      901 ctgaaggagt tcataggctt ttttcgtcac ttcactacct tttgttgctt ctgttccgtt
      961 gtttaagagt gcgacacgag gacgtccgat acctcggact ttacgtgcat aaaaagaacc
     1021 taaaataccg tattgcaaca aatgttctgg tttgttttct gcgttagcgc caagatccag
     1081 catatcgaaa ccggcacctt ctttccctac tactggcaag gtagacatca agccagggcg
     1141 ttcgatattt ttgatacgtc ctacgatgaa caaccctgct gctagtagcg cgcctgtatt
     1201 ccctgctgaa aatacagcat ctgcttcacc gtttttcact gcttgtgcag caaggaccat
     1261 tgatgcagtc ttttttcgtc gaattgcttt tactggctca tcatcactat tgattttttc
     1321 gtctgtatgg ataatcgtga tgtttttttc atttgttaag tatttcttga tttctgcttc
     1381 ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc
     1441 tacaattgct tgaggtgcgt gatctccgcc cattgcatct acggcaattt tcattgtttc
     1501 cactcctttt tccgtcatca gtataaagta tagcacagtc tgctaatcga aataacgatt
     1561 atcctgattt tctatttgga ggctttgcgc taaaggctga tactcaggaa gcatctgcca
     1621 atcctttatc tgccaaaggg ctgcagcctc ttctctggct acctccaaaa catttccatc
     1681 cgtcaccaag tcagcaatcg caaattgagg cagaccagat tgacggaagc caaatacttc
     1741 accaggacca cgtaattcca gatctttttc gcttagtaca aaaccattgg tggtctctgt
     1801 catgattttc atccgttcca cacctagttc atttttagga ttagcaacta agatacagta
     1861 ggaagcatca cttccgcgtc caacacgtcc acgaagttga tgcagctgtg ccaatccaaa
     1921 tcgatccgca tccatgatca gcatgacggt cgcgttcggc acgtttaccc ctacttcaat
     1981 tacagtagtc gaaactaata tctgcatctg gttgtctttg aaggcttcca tgattacttc
     2041 tttttcctga ttcttcattt taccatgtaa caagccaact tcatattttg gtgcaaatag
     2101 ttcacgaagt ttttcataga tttccactgc attttttaca tctaatgctt ctgattcttc
     2161 aatcagcgga cagatgacat acatttgatg tcctcttgcc agttcttttt tcgtccaatc
     2221 cagtacactg tccaattgag gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc
     2281 tggaagttca tcaatgatag atacatccat ctcaccatat gctgtaatag ctaatgttct
     2341 aggaatcgga gtcgctgtca taaataagac atctggtcgc cagccttttt cacgaagtac
     2401 tttccgctgg ttcacgccaa aacggtgctg ctcatccgta ataactagac ctaaattctg
     2461 aaattcgact ccatcttgga tcaatgcatg tgtgccgatc acgatgttaa tttccccttg
     2521 ctctagtttt tcaaggagct cacgacgttc ttttgctttc gttgaccccg taagcaatgc
     2581 gatggttact tcattgggat caaacagctg ttgcaagctt tccatatgtt gttgtgccaa
     2641 gatttctgta ggcaccatca acgcaccttg gaaacctgct gtaacggtag cgtataacgc
     2701 aatcgcggca accactgttt ttccgctacc tacatctccc tgcaaaagtc gctgcatatg
     2761 ctgcggactg cgaagatccc gacagatttc attcgttaca cgtttttgtg catttgtcag
     2821 ttcaaaaggt agcttttgag taaattcttt caatttttgg acatcataaa ggatctccaa
     2881 accattcgtt tctgcttttt ctgctttttt caatccttga agacgcattt gaaataaaaa
     2941 gaactcttca aaaacgacac gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa
     3001 atgcatactt cttacagcag aagctcggtc aagcagtcga tatttctcca caaggttgtt
     3061 tggcagattt tcttcaatca atgtgccgta ttcttgaaac ccttttttga ttagatcaac
     3121 taatgtcgtt tgacgaattg atttgttgac gtgatatatc ggcgaaaagt catccattgt
     3181 ttgagaacct aaaatcttca tcccgttcaa tgctttacgc tttgcatccc atttaccgta
     3241 tacggcgatt tcctccgata aaatgacttt ttcttttaga taaggttgat taaaaaaaga
     3301 aacgttgaac acatcatgtt cttgcatcat acgaaattgc aatcggcttt ttttataacc
     3361 aaatcgattc atcacaggag gcgagactac gattcctttc aaagtaacct tttcttgatc
     3421 ttggatctcg tttagatttc tttcctgaat atcttcgtag cggaaaggat aataagtcag
     3481 taagtcttca atcgtttgga tgcccaagct ggcaagacta gctgctcttt tttctccgac
     3541 tcctgccagc atagtgattg gatcatctaa agaacgcata aatttttcct ccctccctta
     3601 acaaaaaaag gacttggtca gcgatcatac ctgatcacta accgaaggcc ttttatgaat
     3661 gaatactgtg ttagaaagat attctgacac agaaattatt cagcagaaaa tagataagga
     3721 tagacaggct gatctccttg gtggatctcg atttccaaat caggatgttt ttctgttagt
     3781 ttctcagcaa attcatttgc ttgttcaact gttccttcat cacctgccaa gatcgttacg
     3841 atctctgtgt cctcattgat catttgttct agtgtactta acgctgttgc tgtgatatct
     3901 gggttagaga tcaggatttt tccatcgatc atgcccaaaa agtctccttc tttgatctct
     3961 acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt gaccgctcac aacgctagac
     4021 aacatttctg tcatcgtttc tttgttttct tctaaagaag cttgatcatt gaatgaaagc
     4081 atagctgtca atccttggga aatcgttctt gaaggaacaa ctgctactgg gatatctgct
     4141 acttctgctg cttgatcagc tgccatgaag atgttcttat tgtttggcaa gacaatcact
     4201 ttatctgcgt ttacttcttg aatcgcttta acaatgtctt ccgtacttgg attcattgtt
     4261 tgcccaccac tgataacata attagcgcct aaactttcaa acagctcttg gacgccttta
     4321 ccagcagcga tcgctacgat accatacggg atacgtggtg tttctttttt cgcaaactcg
     4381 ttcacttctt catcgtgttc caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg
     4441 attttgacta atgaaccgaa tttttgtcca taattcaaga cttctcctgg gttttctgta
     4501 tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca
     4561 tctaaatagt tacggaattc ttcgtaatcg aatgtactat cgactgtagg tccttctccg
     4621 atttttacca tgatttctgt acagtaacca aaatggatat cttctgtcgc catttgacct
     4681 tggacactgc gatgatgttc tgcattgacc atatcatcca tttctgctgg agaaggttca
     4741 tatgtttcat ccgcttggaa ttctccattc aatgcactta aaaagccctc ataaacaaac
     4801 agcagtcctt gccctccact gtctacgaca ccgacttctt ttaggactgg aagcaagtct
     4861 ggagttttag ctaaagcacg ctttgcccct tttacaacag ctgtcattac ttcaacgaca
     4921 tcatcagtac tttttgcttt cttttctcca gctttcgctg cttctctagc tactgtcaaa
     4981 atcgttcctt ctactggttt cattactgct ttatacgcag tttcaactcc atgagtgaag
     5041 gctgcagcaa gatcggaagc atttaatgag acaacatctg gaatttgctt agaaaatcca
     5101 cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg cacccatcag caatcctttt
     5161 gaaagcattg ctgctaattc tcctactttt tctgaggttg aatctactac tgccttagct
     5221 ccactagtca tagatagatt catatttgtt cccgtatcgc catctggcac agggaataca
     5281 tttaatgaat tgacgtattc tgcatttact tgcagacgat tcgcacctgc ctggaccatt
     5341 tcctggaact ggcttgcact tatttctgtt acactcacct agataatcct ccttcagatc
     5401 gactgctgag ttaccagcca gtctcctgcc tagtctggca gtacacgaac accttgtaca
     5461 aagacattaa ctgaattggc agttacgcct aataatgcct ctagattgta tttcactttt
     5521 tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat agctgataat cgtataaaca
     5581 tctacagcaa taccattatc ttcttggcgg acaactactc ctcttgagta attttcttta
     5641 cgcaaaatct cagtaatatt atctttaatt tgatttttgc tggccattcc tacaacaccg
     5701 aacacatctg tcgcggcgcc accaacaact gttgcaatta catcattggt aatttcaatg
     5761 gttccagctg acgtttgaat ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac
     5821 tcccattttt cttctatcat attttatcat agcgcaactt gaaataaaag gcagttaccc
     5881 aaagtactca aaaaccttgc aaaatttcag gtataacctt gtttaattta caattattct
     5941 tctcaattgt caattgtatc tatgaaaatg tacttgcaaa tttccattct atatgatatg
     6001 ttagtctggt atgagctgaa ttattctatt gctccaaatc aaggcaaagg aggaaattac
     6061 catggcaaaa gtttgttact ttacaggtcg caagacaaaa agtggaaaca accgttcaca
     6121 cgctatgaac tcaacaaaac gtactgttaa acctaactta caaaaagttc gcgttatggt
     6181 cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga
     6241 acgcgtttaa ttgaatgata cttggaccgt tcagatagtg aacggttttt tatttaacag
     6301 aattaggcag aagtcgccca tcctctatag gtgggtgatg aatgccgttc ggtatgaggg
     6361 ttaccgttgg taactcgaag accgacaact aaggcgtatt gcctttttat tttgttccat
     6421 gttacaatca gtctatacaa gaaatgagtt gatataacat ggaattagat acaaataatc
     6481 attcagtatt tcttctttat taccaccttg ttttagtaac gaaatatcgt agacaagtga
     6541 ttgatgatga aatatctgat tatgccaaaa ctacttttga aagaatttca gaatcgtatc
     6601 acattacttt agtggagtgg aatcatgata aagaccacgt tcatattatg ttcaaagctc
     6661 aacctaaaac agaattgaca aaattcatca atgcctataa aagtgccagc tcaagattga
     6721 taaaacgtga ctttccaaga gtcaaacaat ttctttggaa agaaatgttc tggtctaaaa
     6781 gtttttgtct tctgacaact ggtggcgcac caattgatgt gattaagaag tatatccaaa
     6841 atcaaggaaa caatcataaa tagaaagtag gtgaacttta tggaacaact aaaagcatac
     6901 aaattcagaa tctatccaac agaggaacaa gaaatcttct ttgccaaatc ttttggttgt
     6961 gtccgtaagg tctacaatct aatgcttgat gaccgaaaaa aggcgtatga agaagttaaa
     7021 aatgattctt ctaaaaaaat gacttttccg acaccagcta aatacaagaa agaatttccg
     7081 tttttgaaag aaattgatag ccttgcttta gccaatgccc aacttaattt agataaagca
     7141 tacaagaatt tttttcggga taaatccgtt ggatttccac gtttcaaaag taagaaaaat
     7201 cctgttcaga gttatacaac gaataatcaa aatggtacag ttgctttgat tgatagtaaa
     7261 ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta agcttcatag acaaccaaaa
     7321 gggattatca aatctgctac aatatcacgt cattctagcg gtaaatacta tatttctttg
     7381 ttgtgtaaag aagaaattat cgaattacct aaaactaatt ccacaattgg tattgactta
     7441 ggtattatgg actttgcgat tctttctgat ggacaaaaaa ttgataataa taaattcacg
     7501 tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat tgtctaggcg tgctttattg
     7561 gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt atcaaaaaca aaaacgcaaa
     7621 gtagctagat tgcatgaaaa agtaatgaat caacgtactg attttctgaa taagttgagt
     7681 acagaaatga tcaaaaatca cgatattatc tgtattgaag acttaaacac aaaaggtatg
     7741 ttgcgaaatc ataaattagc taaaagtatt tctgatgttt cttggtcaaa gtttgtgact
     7801 aaattacaat acaaggctga ctggtatgga cgaaaaatca tcaaagtaga taaatggttt
     7861 ccgtctagtc agatttgttc agagtgcgga cataaagatg gcaagaaatc tcttgaaatt
     7921 cgagaatgga cttgtcctgt ttgtcatact catcatgatc gtgatatcaa tgctagtatc
     7981 aatattctga ccgaaggtct aagactacat tctatgggat tggcttagat ccaatcatca
     8041 agaaccgtag gaactacggg gatagcttgg taaataagag acaccgctgt tagtaaagaa
     8101 atacgctatc aagtatgctc tattcccaag aagctcccac ttcaagcgtt aagaaccgtc
     8161 aggtttagct aagtggtgag tagttcaccg tttgtttttg caccgttaga aggagaagac
     8221 ctcgcttcat ttctggttgg tgcggcaatc atgatgatcg gaatgacgct ctttttgttt
     8281 ggagcagatt attctatgat ggaagtcgga gatctagtag ggaaatatat gatcaaaaag
     8341 aaaagtttga ccgttttggt cagtttggga tttgctatcg ggatcgtgat cacgattgct
     8401 gaaccttccg tgcaagtttt gggacaacaa gtatatgata tatctgacgg agagattggc
     8461 agagtgttgc tgattgggat cgtcagcgtc ggcactggag tatttctagc ctttgcgctg
     8521 cttcgggtcg tattcaagct atcctattat caattgatgg ctataggcta cataggcgtt
     8581 ttgatcgcct catttttcac cagttccgaa tttatgccga ttgcttttga ttcgggggga
     8641 gtgactactg gtccagtaac tgttccattt atcttggccc ttgccggagg gatgactagt
     8701 atgatcaaac aaaagaaaaa tgaaaatgac agttttggaa tggtcggtat tgcttcgtta
     8761 gggccgattc ttgccgtgat gattttgggg gtgatcttcc aatgagtttc tggcagcaag
     8821 tgatagatgg aatcgaaatc gttaccatgg aagtcatttt ggcagtggca cctatcgtaa
     8881 tcctatttgt tttaatgaat atcattagtt ttcgtatgcg caaaaagcgt ttctttgaca
     8941 ttatgaaagg tttcttgata acgactattg ggttgatcct tttcctgcac ggagtcaata
     9001 tggcttatgt accggtagga cagcatttag gcagtgctat tgcgggattg ccttacaatt
     9061 atatactggt tccccttggc tttttaatgg ggttcttagt agggtttgct gagccggcga
     9121 ttcatgtaat gatcaaacag gttgaagaat tgagcgaggg gagaatccga tcgaaaatca
     9181 tgctgatggt aatttcaatt gggatcggcg cagcagtagg attgtctatg tggcgtttgc
     9241 tcgtcggatt ctctctttat tatttcttga tcccaggata cgggctagca tttattttag
     9301 gacgaaaagt ggacaagatc tttttagcaa tggcttttga taacggtgga gtagcaacgg
     9361 gcccgatgtg ttcgacattt attttatcaa tgagcgtggc aatcgctgca caaattgaag
     9421 gaagagatcc gatcattgac gggtttggtg tggttgcaat gattgcattg acaccaatat
     9481 tatccacttt agtactagga tatatttata aacgaaaaga tgaaaaagac agaatcgaaa
     9541 aagaaagatt gcaaaaattg ggggaaagca aatgaaagat cttgtagcat tgaatcttca
     9601 catgattgtg acgatcgttg acggaggaat gggcgaagat gttatcgact tttcaaaaga
     9661 agcaggtgca gcaggcggaa cgatcttaca tggaagaggt tctggtgtac atgattcagg
     9721 gaaattttta ggtttggaca tttcgccaga aaaagatatt gtgttgacat tagtacccga
     9781 tagtttgacg aataaagtaa tggatgcgat tggtcaaggc atcaacatcg atattccagg
     9841 taatggtatt tgttttagta ttgatgtaga caaagtcatt ggtatcacta agattcatca
     9901 atatcgagaa attatcggaa tgcaagaact tttagatgaa aataacgata gagaaaagtc
     9961 tgaatcatag acaagcgttt gaagaggaaa taaacacaaa caagaaggcg gatgagcgtt
    10021 atttccgctt ttttgtttgt atttttctcg tgaatactta tgaaatagta aatttttatg
    10081 aaattctact ctaagcatca gactttcttt tcaaaatatg ctattttaaa aatgatgaaa
    10141 aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt gagcgattta
    10201 catttctata tgttcaattg caggaaaata aaacattgct gcatttttca ccagagttcc
    10261 atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg gctgttttgc
    10321 acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt tctcttgaat
    10381 cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat acaaacgatg
    10441 ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa atagatgacg
    10501 aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt ttaaaagtga
    10561 tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat ccaggtctgc
    10621 ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt atacgtatgc
    10681 aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga gtagtcaaca
    10741 gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat gtccttcacc
    10801 aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt gtcataataa
    10861 atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct aatacgattt
    10921 cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca gttgaatcgc
    10981 ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc ctatagaatc
    11041 ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct atcaaaaaaa
    11101 atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag agtcaaagaa
    11161 aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag ttatactttt
    11221 tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg acgaatacgt
    11281 gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag aaaagaatga
    11341 aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac tggacaggat
    11401 ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc ggtcaaaaaa
    11461 tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa cgtgcgattc
    11521 ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata ataaaaacat
    11581 ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat aagatgaaag
    11641 aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa gttccgccgt
    11701 taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat ttgatcccat
    11761 acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa gatcatctag
    11821 caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat agcatccgag
    11881 cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc gatatcccaa
    11941 atgcacaacc aatccagtat acattagcag atgatttgac aattctggac aaaaaaatac
    12001 tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag gaatggaaca
    12061 aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat acgtatcccg
    12121 caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg gacaaaaaag
    12181 aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa tcaaacttga
    12241 atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg gaagtttccc
    12301 gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc ggtcgtcatt
    12361 tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa catcgattgg
    12421 cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa cctgatccag
    12481 catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg atgatgatcg
    12541 gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact tgtctttacg
    12601 acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat ctgaatgaaa
    12661 tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag atatgttata
    12721 attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga agttgtagaa
    12781 gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt gaaagtaggc
    12841 ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat cgtaggagca
    12901 cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt gagtcagatc
    12961 aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga agaagtggac
    13021 gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt gacagagatc
    13081 gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga atagacagta
    13141 aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta ctaattgtaa
    13201 gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact gaaaaaaatt
    13261 ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc gatggacttg
    13321 tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta gtggttctag
    13381 tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca aaattttcaa
    13441 cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt caattgatct
    13501 ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt gtaggtcaag
    13561 gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg atcattggtg
    13621 cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact gtattgactt
    13681 ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca atgctgttcg
    13741 gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc tctgaactgg
    13801 caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta tcctttatcc
    13861 ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt ggagtcgtta
    13921 ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg ccaaaagaag
    13981 cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg atcgtgacgc
    14041 ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt ttggcaacaa
    14101 gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac gtagcattgc
    14161 ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat ccaatcaact
    14221 atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca agtgctggag
    14281 ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat gcaacagcta
    14341 atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc gtttatccag
    14401 ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt ttggaattta
    14461 ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga gtcggtgggg
    14521 gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta gcaatcgttg
    14581 ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta aacgtcagcg
    14641 gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat cgcgatgtca
    14701 tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt tgtgatagaa
    14761 gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa atttttgtga
    14821 aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag gttgtgaaaa
    14881 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    14941 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg
    15001 agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt ctcatgtttg
    15061 ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac cagaggttgt
    15121 gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca c
//
LOCUS       DACCZY010000067        14973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCZY010000067 DACCZY010000000
VERSION     DACCZY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.067"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            15..1145
                     /gene="dnaN"
                     /locus_tag="IVZ67_001753"
     CDS             15..1145
                     /gene="dnaN"
                     /locus_tag="IVZ67_001753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8165830.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1374..1616
                     /gene="yaaA"
                     /locus_tag="IVZ67_001754"
     CDS             1374..1616
                     /gene="yaaA"
                     /locus_tag="IVZ67_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8165831.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            1603..2727
                     /gene="recF"
                     /locus_tag="IVZ67_001755"
     CDS             1603..2727
                     /gene="recF"
                     /locus_tag="IVZ67_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8165832.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            2724..4670
                     /gene="gyrB"
                     /locus_tag="IVZ67_001756"
     CDS             2724..4670
                     /gene="gyrB"
                     /locus_tag="IVZ67_001756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8165833.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            4690..7161
                     /gene="gyrA"
                     /locus_tag="IVZ67_001757"
     CDS             4690..7161
                     /gene="gyrA"
                     /locus_tag="IVZ67_001757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8165834.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            7397..7696
                     /locus_tag="IVZ67_001758"
     CDS             7397..7696
                     /locus_tag="IVZ67_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8165835.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            7746..8258
                     /gene="ssb"
                     /locus_tag="IVZ67_001759"
     CDS             7746..8258
                     /gene="ssb"
                     /locus_tag="IVZ67_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8165836.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            8284..8520
                     /gene="rpsR"
                     /locus_tag="IVZ67_001760"
     CDS             8284..8520
                     /gene="rpsR"
                     /locus_tag="IVZ67_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8165837.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            8663..10636
                     /locus_tag="IVZ67_001761"
     CDS             8663..10636
                     /locus_tag="IVZ67_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8165838.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            10642..11094
                     /gene="rplI"
                     /locus_tag="IVZ67_001762"
     CDS             10642..11094
                     /gene="rplI"
                     /locus_tag="IVZ67_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8165839.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            11368..12735
                     /gene="dnaB"
                     /locus_tag="IVZ67_001763"
     CDS             11368..12735
                     /gene="dnaB"
                     /locus_tag="IVZ67_001763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8165840.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            13515..14807
                     /locus_tag="IVZ67_001764"
     CDS             13515..14807
                     /locus_tag="IVZ67_001764"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8165841.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4985 a         2696 c         3244 g         4048 t
ORIGIN      
        1 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
       61 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
      121 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
      181 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
      241 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
      301 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
      361 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
      421 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
      481 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
      541 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
      601 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
      661 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
      721 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
      781 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
      841 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
      901 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
      961 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
     1021 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
     1081 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
     1141 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
     1201 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
     1261 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
     1321 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
     1381 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
     1441 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
     1501 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
     1561 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
     1621 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
     1681 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
     1741 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
     1801 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
     1861 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
     1921 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
     1981 agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
     2041 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
     2101 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
     2161 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
     2221 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
     2281 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
     2341 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
     2401 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
     2461 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
     2521 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
     2581 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
     2641 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
     2701 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
     2761 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
     2821 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
     2881 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
     2941 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
     3001 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
     3061 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgtaaacg
     3121 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
     3181 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
     3241 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
     3301 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
     3361 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatca
     3421 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
     3481 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
     3541 attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga acacatgaat
     3601 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
     3661 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
     3721 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
     3781 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
     3841 aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc agagctcgtc
     3901 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
     3961 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
     4021 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
     4081 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
     4141 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
     4201 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
     4261 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
     4321 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
     4381 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
     4441 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
     4501 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
     4561 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
     4621 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
     4681 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
     4741 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
     4801 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
     4861 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
     4921 taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc acagccattc
     4981 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
     5041 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
     5101 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
     5161 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
     5221 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
     5281 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
     5341 tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt
     5401 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
     5461 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
     5521 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
     5581 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg
     5641 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
     5701 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat
     5761 caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga agcaagagca
     5821 cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
     5881 cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc
     5941 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
     6001 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
     6061 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
     6121 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
     6181 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
     6241 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
     6301 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
     6361 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
     6421 gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc gagtgaaagc
     6481 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
     6541 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
     6601 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
     6661 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
     6721 gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa
     6781 gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt gatcacagaa
     6841 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
     6901 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
     6961 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
     7021 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
     7081 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
     7141 gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa gaataaccga
     7201 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
     7261 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta gagcttgtga
     7321 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
     7381 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
     7441 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
     7501 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
     7561 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
     7621 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
     7681 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
     7741 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
     7801 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
     7861 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
     7921 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
     7981 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
     8041 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
     8101 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
     8161 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
     8221 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
     8281 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
     8341 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
     8401 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
     8461 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
     8521 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
     8581 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
     8641 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
     8701 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
     8761 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
     8821 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
     8881 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
     8941 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
     9001 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
     9061 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
     9121 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
     9181 tggtatttta tctattgata actatgcaga tgccattgat aaattagatg acaaagagat
     9241 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
     9301 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
     9361 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
     9421 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
     9481 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
     9541 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
     9601 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
     9661 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
     9721 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
     9781 tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
     9841 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
     9901 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
     9961 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    10021 tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    10081 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    10141 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    10201 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    10261 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    10321 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    10381 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    10441 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    10501 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    10561 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    10621 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    10681 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    10741 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    10801 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    10861 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    10921 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    10981 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    11041 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    11101 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    11161 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    11221 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    11281 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    11341 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    11401 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    11461 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    11521 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    11581 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    11641 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    11701 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    11761 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    11821 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    11881 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    11941 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    12001 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    12061 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    12121 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    12181 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    12241 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    12301 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    12361 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    12421 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    12481 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    12541 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    12601 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    12661 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    12721 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    12781 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    12841 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    12901 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    12961 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    13021 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    13081 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    13141 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    13201 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    13261 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    13321 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    13381 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    13441 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    13501 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    13561 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    13621 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    13681 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    13741 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    13801 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    13861 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    13921 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    13981 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    14041 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    14101 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    14161 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    14221 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    14281 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    14341 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    14401 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    14461 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    14521 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    14581 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    14641 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    14701 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    14761 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    14821 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    14881 acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt ctcttgaaca
    14941 ctgtttattc agaataaggg actgtgacaa aaa
//
LOCUS       DACCZY010000068        14659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCZY010000068 DACCZY010000000
VERSION     DACCZY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.069"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            107..1003
                     /locus_tag="IVZ67_001765"
     CDS             107..1003
                     /locus_tag="IVZ67_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8165842.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1153..2094)
                     /locus_tag="IVZ67_001766"
     CDS             complement(1153..2094)
                     /locus_tag="IVZ67_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8165843.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2220..3341
                     /locus_tag="IVZ67_001767"
     CDS             2220..3341
                     /locus_tag="IVZ67_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8165844.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3599..5101
                     /locus_tag="IVZ67_001768"
     CDS             3599..5101
                     /locus_tag="IVZ67_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8165845.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5168..5695
                     /locus_tag="IVZ67_001769"
     CDS             5168..5695
                     /locus_tag="IVZ67_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8165846.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5906..6967
                     /locus_tag="IVZ67_001770"
     CDS             5906..6967
                     /locus_tag="IVZ67_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8165847.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6970..7866
                     /gene="murQ"
                     /locus_tag="IVZ67_001771"
     CDS             6970..7866
                     /gene="murQ"
                     /locus_tag="IVZ67_001771"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8165848.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7880..9862
                     /locus_tag="IVZ67_001772"
     CDS             7880..9862
                     /locus_tag="IVZ67_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8165849.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9927..10373)
                     /gene="nrdI"
                     /locus_tag="IVZ67_001773"
     CDS             complement(9927..10373)
                     /gene="nrdI"
                     /locus_tag="IVZ67_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8165850.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10584..12008
                     /locus_tag="IVZ67_001774"
     CDS             10584..12008
                     /locus_tag="IVZ67_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8165851.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12189..12962)
                     /locus_tag="IVZ67_001775"
     CDS             complement(12189..12962)
                     /locus_tag="IVZ67_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8165852.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12959..13864)
                     /locus_tag="IVZ67_001776"
     CDS             complement(12959..13864)
                     /locus_tag="IVZ67_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165853.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14018..14338)
                     /locus_tag="IVZ67_001777"
     CDS             complement(14018..14338)
                     /locus_tag="IVZ67_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8165854.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4690 a         2611 c         3100 g         4258 t
ORIGIN      
        1 tacaaatgtt aaattttcat acaaatatag gaaacgtttt actggctttt tactatttaa
       61 acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga acaaaaatga
      121 cgtactgcta gttgcaaaaa ataaatcact ttattttttg ccttccatcc gctctttaga
      181 tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagcta gacaacagtt
      241 ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga tggctcgacg
      301 cgtgcgccca tttaccaatg caaaaattgt gttatttttt tggaatcact tcaaagcaga
      361 acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga tttatcattt
      421 tgattttttg gaagcaaaag aacttgggct gaaacataat agttcatttt attcgaaaca
      481 tatgaatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa cgaataatgg
      541 gagaaaagaa cgagcagaaa gctataagca ggaatttata aaaagagatg ttacgaccaa
      601 ctatttcatt cttccacatc gagggaatga acaacctgga tatcttactt atgaagagta
      661 cctgaaaaaa acaggggaat cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg
      721 agtgactttg cggacatttg aatccatgta ttttcaaaag aaactagtga cggataatca
      781 agcgattgct cattatcatt tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga
      841 tttagatgaa ttaccggaat ttctgcatac accatttaag ccaatcctat cagagctatt
      901 ggaattttat gatgcagaaa attgggcaaa acgatttatc actaacgatt ccactattta
      961 tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct acattttgaa
     1021 gagattatgt actgaaacaa acaagcacaa ttcttatggc atcatgagag ttgtgttttc
     1081 tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca
     1141 tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa
     1201 aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt
     1261 gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta
     1321 atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg
     1381 atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac
     1441 catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca
     1501 taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg
     1561 gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc
     1621 ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt
     1681 tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg
     1741 cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt
     1801 attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt
     1861 taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat
     1921 tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat
     1981 ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag
     2041 catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt
     2101 tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt
     2161 gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga
     2221 tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc
     2281 aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag
     2341 atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca
     2401 ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc
     2461 aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata
     2521 aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag
     2581 atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg
     2641 atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa
     2701 atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag
     2761 taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt
     2821 ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag
     2881 caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag
     2941 gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt
     3001 ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg
     3061 tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg
     3121 gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
     3181 accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc
     3241 caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag
     3301 gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt
     3361 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
     3421 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga
     3481 ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg
     3541 catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat
     3601 gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt
     3661 tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     3721 tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt
     3781 aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac
     3841 ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca
     3901 attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga
     3961 gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga
     4021 tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa
     4081 gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga
     4141 ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta
     4201 tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa
     4261 agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga
     4321 aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa
     4381 aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg
     4441 gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc
     4501 tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca
     4561 attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg
     4621 ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg
     4681 ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta
     4741 tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca
     4801 agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt
     4861 tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc
     4921 tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt
     4981 caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga
     5041 acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta
     5101 acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga
     5161 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     5221 ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc
     5281 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     5341 agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga
     5401 acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt
     5461 tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt
     5521 gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct
     5581 gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa
     5641 tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg agtaattatg
     5701 tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt
     5761 taggttatga caattctttt gataatataa actcaacctt tagaaatgaa atttaattat
     5821 tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag
     5881 acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt
     5941 gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt
     6001 cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact
     6061 aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt
     6121 ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg
     6181 tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa
     6241 aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga
     6301 agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct
     6361 ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca
     6421 aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt
     6481 ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac
     6541 ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc
     6601 tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga
     6661 atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt
     6721 agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa
     6781 agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac
     6841 caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga
     6901 gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg
     6961 aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa
     7021 acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat
     7081 cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac
     7141 acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga
     7201 agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg
     7261 gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa
     7321 gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata
     7381 gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca
     7441 aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa
     7501 cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga
     7561 ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt
     7621 ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta
     7681 atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa
     7741 aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat
     7801 agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc
     7861 ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca
     7921 aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg
     7981 tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc
     8041 aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt
     8101 gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc
     8161 ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg
     8221 ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt
     8281 attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat
     8341 cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc
     8401 aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct
     8461 agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg
     8521 agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt
     8581 atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt
     8641 ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat
     8701 catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat
     8761 gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt
     8821 tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg
     8881 actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct
     8941 attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg
     9001 gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg
     9061 gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt
     9121 tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt
     9181 cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa
     9241 atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt
     9301 cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac
     9361 agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct
     9421 ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc
     9481 acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc
     9541 agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc
     9601 aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc
     9661 atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc
     9721 tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa
     9781 tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc
     9841 tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa
     9901 tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt
     9961 cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat
    10021 gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta
    10081 ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt
    10141 tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga
    10201 acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt
    10261 aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata ttctgctaga
    10321 tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat
    10381 cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg
    10441 aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat
    10501 taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg
    10561 gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa
    10621 ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga
    10681 cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag
    10741 caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag
    10801 gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt
    10861 ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg
    10921 cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga
    10981 gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt
    11041 tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag
    11101 ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac attatattga
    11161 atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc
    11221 tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat
    11281 cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg
    11341 tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc
    11401 ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg
    11461 gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa
    11521 caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa
    11581 atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt
    11641 cattattatt cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt
    11701 tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat
    11761 gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg
    11821 tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc
    11881 tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac
    11941 ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc
    12001 acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa
    12061 catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg
    12121 tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg
    12181 atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg
    12241 atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg
    12301 atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc
    12361 cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa
    12421 aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc aatcatcaac
    12481 actaaactta aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt
    12541 tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc
    12601 agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga ataaataact
    12661 aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata aaggagattt
    12721 ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata
    12781 aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc
    12841 gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat
    12901 aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc
    12961 atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat
    13021 tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg
    13081 ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat
    13141 atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga
    13201 aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat
    13261 tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca
    13321 agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt
    13381 gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg
    13441 caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa
    13501 tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt
    13561 cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga
    13621 tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa
    13681 tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt
    13741 ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga
    13801 catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt
    13861 tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag
    13921 tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa
    13981 aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga
    14041 tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat
    14101 catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag
    14161 gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa
    14221 tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca
    14281 tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac
    14341 ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga
    14401 cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat ctttttctgt
    14461 agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc
    14521 actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa
    14581 gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct atatattatc
    14641 gggcaataaa ttaaaaaaa
//
LOCUS       DACCZY010000069        14613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCZY010000069 DACCZY010000000
VERSION     DACCZY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.070"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..1024)
                     /locus_tag="IVZ67_001778"
     CDS             complement(296..1024)
                     /locus_tag="IVZ67_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8165855.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVZ67_001779"
     CDS             complement(1002..2576)
                     /locus_tag="IVZ67_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8165856.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVZ67_001780"
     CDS             2805..3743
                     /locus_tag="IVZ67_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8165857.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVZ67_001781"
     CDS             3755..4666
                     /locus_tag="IVZ67_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8165858.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVZ67_001782"
     CDS             4690..6162
                     /locus_tag="IVZ67_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8165859.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IVZ67_001783"
     CDS             6213..6824
                     /locus_tag="IVZ67_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8165860.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IVZ67_001784"
     CDS             6854..8767
                     /locus_tag="IVZ67_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8165861.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IVZ67_001785"
     CDS             8895..11876
                     /locus_tag="IVZ67_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8165862.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IVZ67_001786"
     CDS             11873..13534
                     /locus_tag="IVZ67_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8165863.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IVZ67_001787"
     CDS             13574..14506
                     /locus_tag="IVZ67_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8165864.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4746 a         2300 c         2936 g         4631 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aat
//
LOCUS       DACCZY010000070        14595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCZY010000070 DACCZY010000000
VERSION     DACCZY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.071"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(184..750)
                     /locus_tag="IVZ67_001788"
     CDS             complement(184..750)
                     /locus_tag="IVZ67_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8165865.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(750..1706)
                     /locus_tag="IVZ67_001789"
     CDS             complement(750..1706)
                     /locus_tag="IVZ67_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8165866.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1706..2362)
                     /locus_tag="IVZ67_001790"
     CDS             complement(1706..2362)
                     /locus_tag="IVZ67_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8165867.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2732..4750
                     /locus_tag="IVZ67_001791"
     CDS             2732..4750
                     /locus_tag="IVZ67_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8165868.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5177..7591
                     /locus_tag="IVZ67_001792"
     CDS             5177..7591
                     /locus_tag="IVZ67_001792"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8165869.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7939..9492
                     /locus_tag="IVZ67_001793"
     CDS             7939..9492
                     /locus_tag="IVZ67_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8165870.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9873..11657)
                     /locus_tag="IVZ67_001794"
     CDS             complement(9873..11657)
                     /locus_tag="IVZ67_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165871.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11672..13393)
                     /locus_tag="IVZ67_001795"
     CDS             complement(11672..13393)
                     /locus_tag="IVZ67_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8165872.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13493..14254
                     /locus_tag="IVZ67_001796"
     CDS             13493..14254
                     /locus_tag="IVZ67_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8165873.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4738 a         2700 c         2910 g         4247 t
ORIGIN      
        1 ctgcatcaag taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg
       61 gacttattac cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac
      121 gctctaacct gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt
      181 tatttatctt tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa
      241 ccgataattc agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat
      301 atccagttct ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg
      361 aggcacaagt cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat
      421 gattgcttta tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg
      481 ttgttcagct atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc
      541 ttctaactga cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc
      601 aaaactatag acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc
      661 attgcccatc gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt
      721 atggctgaaa tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat
      781 tttccttgga agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat
      841 tttttcaagc tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga
      901 tggatgatga tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca
      961 ggacggctca ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc
     1021 atcagatgca tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg
     1081 acattttctt tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa
     1141 acacggatat tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt
     1201 gctcggttga tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat
     1261 aaatacatcc gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc
     1321 ggtcgtgtac cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt
     1381 tctttgatta tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat
     1441 tgttggacat gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga
     1501 agagggtctt caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa
     1561 ccgccatggg caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc
     1621 ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta
     1681 tttccttctg aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt
     1741 gttttctttt ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt
     1801 aaccgccgat gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac
     1861 ttactataat aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa
     1921 aagctggaag cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac
     1981 tagggaaact gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga
     2041 aaaatagttt gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc
     2101 aaaatgtttc tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa
     2161 tgatgacggt aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag
     2221 gataagaagc tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat
     2281 ggaactttac gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct
     2341 gaaatgatgt tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt
     2401 tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata
     2461 accaatattc ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat
     2521 gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg
     2581 taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca
     2641 aaaaacaaca aagctcttaa ataactttta ataaatatta agagaagtta taattattaa
     2701 taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag
     2761 acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac
     2821 tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga
     2881 tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc
     2941 tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca
     3001 agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct
     3061 ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt
     3121 aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc
     3181 tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc
     3241 cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg
     3301 ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt
     3361 gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa
     3421 taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg
     3481 ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa
     3541 taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat
     3601 ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa
     3661 ttccggtaac aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac
     3721 tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg
     3781 aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg
     3841 acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa
     3901 tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg
     3961 aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat
     4021 tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca
     4081 acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc
     4141 aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta
     4201 taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga
     4261 tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat
     4321 cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc
     4381 aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac
     4441 cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca
     4501 gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt
     4561 gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac
     4621 tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca
     4681 actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt
     4741 agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac
     4801 tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc
     4861 agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc
     4921 cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt
     4981 tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt
     5041 ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa
     5101 ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag
     5161 aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata
     5221 ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc
     5281 actggatatg tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta
     5341 tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc
     5401 aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga
     5461 tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg
     5521 atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac
     5581 acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt
     5641 aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag
     5701 tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac
     5761 tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa
     5821 agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc
     5881 tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac
     5941 ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa
     6001 agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga
     6061 tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg
     6121 caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat
     6181 catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga
     6241 aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac
     6301 gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa
     6361 tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg
     6421 gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg
     6481 aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat
     6541 caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt
     6601 tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg
     6661 aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta
     6721 tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt
     6781 acttcattta ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc
     6841 tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga
     6901 aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg
     6961 tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg
     7021 gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt
     7081 tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa
     7141 agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac
     7201 agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta
     7261 tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga
     7321 actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga
     7381 tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg
     7441 tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc
     7501 caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc
     7561 aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag
     7621 ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt
     7681 tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca
     7741 atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta
     7801 ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat accaaattca
     7861 taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta
     7921 tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa
     7981 caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc
     8041 ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg
     8101 agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg
     8161 tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc
     8221 cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt
     8281 ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg
     8341 agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat
     8401 ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt
     8461 ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca
     8521 actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat
     8581 tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca
     8641 atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct
     8701 aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg
     8761 cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg
     8821 caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg
     8881 atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat
     8941 gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc
     9001 caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt
     9061 atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat
     9121 ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc
     9181 agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac
     9241 aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg
     9301 gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag
     9361 acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc
     9421 ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa
     9481 caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca
     9541 aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca
     9601 agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat
     9661 tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt
     9721 tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc
     9781 tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca
     9841 tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa
     9901 agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata
     9961 tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca
    10021 atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct
    10081 gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga
    10141 atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta
    10201 gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt
    10261 tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca
    10321 gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt
    10381 tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt
    10441 tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg
    10501 atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct
    10561 gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt
    10621 cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg
    10681 attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca
    10741 gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat
    10801 agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga
    10861 atggccggat agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt
    10921 ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact
    10981 agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc
    11041 tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg
    11101 atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca
    11161 gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa
    11221 agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa
    11281 aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc
    11341 ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact
    11401 gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa
    11461 agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg
    11521 atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa
    11581 aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac
    11641 gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct
    11701 gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt
    11761 catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca
    11821 cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat
    11881 agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc
    11941 gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa
    12001 agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg
    12061 ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg
    12121 tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt
    12181 gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa
    12241 gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg
    12301 ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag
    12361 tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag
    12421 ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc
    12481 gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt
    12541 aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc
    12601 cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca
    12661 aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat
    12721 agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga
    12781 tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct
    12841 ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa
    12901 aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa
    12961 tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt
    13021 gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac
    13081 tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc
    13141 caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta
    13201 gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg
    13261 ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt
    13321 ctaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt
    13381 ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag
    13441 taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa
    13501 aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta
    13561 ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt
    13621 tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc
    13681 gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga
    13741 tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat
    13801 aagggagcaa aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt
    13861 agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa
    13921 aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca
    13981 gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat
    14041 ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca
    14101 tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac
    14161 aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga
    14221 atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa
    14281 aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg
    14341 gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa
    14401 atggaatggt tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg
    14461 gaaaaataat tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga
    14521 aaaattcata ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc
    14581 ataaaagcat tacac
//
LOCUS       DACCZY010000071        14459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCZY010000071 DACCZY010000000
VERSION     DACCZY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.072"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            624..1487
                     /locus_tag="IVZ67_001797"
     CDS             624..1487
                     /locus_tag="IVZ67_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8165874.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1616..3238)
                     /locus_tag="IVZ67_001798"
     CDS             complement(1616..3238)
                     /locus_tag="IVZ67_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165875.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3487..4008
                     /locus_tag="IVZ67_001799"
     CDS             3487..4008
                     /locus_tag="IVZ67_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165876.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVZ67_001800"
     CDS             complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="IVZ67_001800"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8165877.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6089..6907
                     /locus_tag="IVZ67_001801"
     CDS             6089..6907
                     /locus_tag="IVZ67_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8165878.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7010..7207)
                     /locus_tag="IVZ67_001802"
     CDS             complement(7010..7207)
                     /locus_tag="IVZ67_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165879.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7376..7954
                     /locus_tag="IVZ67_001803"
     CDS             7376..7954
                     /locus_tag="IVZ67_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8165880.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8999..9502)
                     /locus_tag="IVZ67_001804"
     CDS             complement(8999..9502)
                     /locus_tag="IVZ67_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8165881.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9861..10886
                     /locus_tag="IVZ67_001805"
     CDS             9861..10886
                     /locus_tag="IVZ67_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8165882.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11209..12087
                     /locus_tag="IVZ67_001806"
                     /pseudo
     CDS             11209..12087
                     /locus_tag="IVZ67_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12563..12679
                     /locus_tag="IVZ67_001807"
     CDS             12563..12679
                     /locus_tag="IVZ67_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8165883.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13607..13945
                     /locus_tag="IVZ67_001808"
     CDS             13607..13945
                     /locus_tag="IVZ67_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165884.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14039..14269
                     /locus_tag="IVZ67_001809"
     CDS             14039..14269
                     /locus_tag="IVZ67_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165885.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4585 a         2422 c         2757 g         4695 t
ORIGIN      
        1 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
       61 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
      121 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
      241 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
      301 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
      361 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
      421 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      481 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
      541 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
      601 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
      661 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
      721 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
      781 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
      841 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
      901 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
      961 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
     1021 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
     1081 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
     1141 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
     1201 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
     1261 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
     1321 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
     1381 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
     1441 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
     1501 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
     1561 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
     1621 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
     1681 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
     1741 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
     1801 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
     1861 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
     1921 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
     1981 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
     2041 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
     2101 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
     2161 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
     2221 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
     2281 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
     2341 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
     2401 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
     2461 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
     2521 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
     2581 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
     2641 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
     2701 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
     2761 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
     2821 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
     2881 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
     2941 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
     3001 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
     3061 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
     3121 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
     3181 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
     3241 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
     3301 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
     3361 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
     3421 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
     3481 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
     3541 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
     3601 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
     3661 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
     3721 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
     3781 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
     3841 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
     3901 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
     3961 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
     4021 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
     4081 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
     4141 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
     4201 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
     4261 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
     4321 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
     4381 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
     4441 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
     4501 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
     4561 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
     4621 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
     4681 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
     4741 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
     4801 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
     4861 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
     4921 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
     4981 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
     5041 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
     5101 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
     5161 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
     5221 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
     5281 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
     5341 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
     5401 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
     5461 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
     5521 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
     5581 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
     5641 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
     5701 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
     5761 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
     5821 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
     5881 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
     5941 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
     6001 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
     6061 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
     6121 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
     6181 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
     6241 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
     6301 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
     6361 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
     6421 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
     6481 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
     6541 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
     6601 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
     6661 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
     6721 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
     6781 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
     6841 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
     6901 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
     6961 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
     7021 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
     7081 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
     7141 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
     7201 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
     7261 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
     7321 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
     7381 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
     7441 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
     7501 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
     7561 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
     7621 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
     7681 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
     7741 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
     7801 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
     7861 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
     7921 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
     7981 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
     8041 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
     8101 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
     8161 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
     8221 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
     8281 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
     8341 cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga
     8401 gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt
     8461 ttttggaata aaaaaataac atatctcatt ttacactttt gttattttat acttaaaaaa
     8521 taacgacaca gaaaataccg tgtctcagat ttctctacta cttctaaaaa ctactgtttt
     8581 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
     8641 gaagggagcc taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca
     8701 agctttttat agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc
     8761 tagattgaga tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa
     8821 ccctttttgt taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct
     8881 gcaataatat agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa
     8941 agaccgtcat agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt
     9001 attgaagacg gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa
     9061 tgtatcccaa aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa
     9121 gttgataggc ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt
     9181 ccgctacgcc aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa
     9241 taatccctaa ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa
     9301 aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag
     9361 aaaaagtaga aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa
     9421 gattaaatga tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata
     9481 aaaattttga tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac
     9541 cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat
     9601 tagaagcttt tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct
     9661 tggaaaagtt gctatctttt ttctgtcaaa acgactcgga atgttcgttt tttgttctaa
     9721 tttctccaag ttgtttactt tttgatacta agtttatgaa aaatcaagaa atagttgtta
     9781 tactaggtaa tgctgacaaa cgggattggc atttcatctt ggttcagctt tttttccaat
     9841 tactaggaag gagggataga ttgttcaaga cttattttcg tttcaataaa atacaagata
     9901 atccttttcg tatcatgaat ctgatgctac ttatctttat tgtattatgg ttaggttaca
     9961 taaatcatga ttttttgatt tcaagttttt catcattagg gatctttact tttttctatt
    10021 atcaaaacat accgatgaaa tatttgctga aacgtatgtt tctgatcggt tgcggactgt
    10081 tggttgcttt cgttttgggg atcttatcga cttatgtttt atggcttgaa ccatttgccg
    10141 tagctttggt tgctttttcc agcagattcg tgttgcgatt attccatatt tctaaaccag
    10201 gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag catgcagctg ccaatcgctc
    10261 aattaccgat cgtctcactt tattttttta tgggtgtcgt ttttgccctg attgcggcag
    10321 tgattacaaa attactggat tcacggcctg agcagacaat tgaaaaagca actttaaaag
    10381 aaagatttca cgaagagcct ttagtgatca ttgattctgt tttctacagc gctgccttat
    10441 ttctttccgt ttatgtcagt cacggcttga atctgcataa tccttattgg ttgaccttgt
    10501 catgtgcatc gattttgcta gcagaaaact tggatgcgat gaagcatcga caagtccaat
    10561 atctgattgg atcgatgggt gggttatgtg tgtcggcatt cttgtcattt gtgccgttta
    10621 cgcagttgca gaccattttt ctgatcactt ttctatatgg aatcgcacaa tttctagttg
    10681 ctagaaatta tgcagttgct aatatttttc ttaatccgat ggcgttgatg ctatctacac
    10741 ttattcgtgg agcatacttg atttctttga tcgaatatcg ttttttaggg atcgtcatag
    10801 gaagtttcat cggtttaggt gttgcgtggg taatgacggt tggtttgcaa cattatttaa
    10861 cggttgttcg tcaaaaatta gaatagaagg acaaatatat gtgccaaaag aggttatgac
    10921 aagatttttg tcacaacctc taattcaagg tgttcaaaag ccagattctc agcattaagt
    10981 caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt attctatcaa
    11041 aaaattttgt ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg
    11101 aaagtcttac agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata
    11161 aaagtatgtt tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa
    11221 gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa
    11281 actttaactc ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt
    11341 gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact
    11401 gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta
    11461 tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc
    11521 tatttggcaa caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga
    11581 agctatcgct ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg
    11641 attggtgaat tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta
    11701 gaaggaaagc agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag
    11761 ataagagata gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat
    11821 taccaagtga tgtacacaga tacttggtta ctatcaccca atcttactaa atagttgaaa
    11881 aaagagtcaa aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag
    11941 gacgatagtg gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca
    12001 gaaagtacga gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc
    12061 tctggcttag gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg
    12121 agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag
    12181 aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa
    12241 tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc
    12301 gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga
    12361 caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg
    12421 agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat
    12481 accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca
    12541 aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt
    12601 gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg
    12661 gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt
    12721 ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt
    12781 tccattttta acatatgata aattggtaga atctttcttt tcaaattgat accattccct
    12841 aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat
    12901 ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt
    12961 taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact
    13021 acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac
    13081 ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg
    13141 ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg
    13201 tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag
    13261 ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat aatagtcgat
    13321 aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa ttttgtgaga
    13381 atcactatta caagtatctt taataaaatt aatggttaag gaggaactta aagtagaatg
    13441 taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca ggaataagtt
    13501 tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt atactttgtt
    13561 gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga ctcaaccagt
    13621 ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga aatatcagga
    13681 aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg atggaaacca
    13741 aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa
    13801 tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa cgtatattta
    13861 tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa ttgctactga
    13921 taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat agaaattgat
    13981 gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc tattcgatat
    14041 gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag ttagagaaga
    14101 tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc cgacatttac
    14161 agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc
    14221 tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag ggagttctta
    14281 ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg ctgttaatat
    14341 tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact tttgtatatt
    14401 tagtaagtag ctacacactt ctatataaat tcagaagttc ttctagagtt tctaaagtt
//
LOCUS       DACCZY010000072        14181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCZY010000072 DACCZY010000000
VERSION     DACCZY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.073"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..475
                     /locus_tag="IVZ67_001810"
     CDS             <1..475
                     /locus_tag="IVZ67_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8165886.1"
                     /translation="LEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALN
                     QWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILR
                     RNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            981..1583
                     /locus_tag="IVZ67_001811"
     CDS             981..1583
                     /locus_tag="IVZ67_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8165887.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1599..2276
                     /locus_tag="IVZ67_001812"
                     /pseudo
     CDS             1599..2276
                     /locus_tag="IVZ67_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2788..3084
                     /locus_tag="IVZ67_001813"
     CDS             2788..3084
                     /locus_tag="IVZ67_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8165888.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3085..3501
                     /locus_tag="IVZ67_001814"
     CDS             3085..3501
                     /locus_tag="IVZ67_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8165889.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3521..5065
                     /locus_tag="IVZ67_001815"
     CDS             3521..5065
                     /locus_tag="IVZ67_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8165890.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5208..5432
                     /locus_tag="IVZ67_001816"
     CDS             5208..5432
                     /locus_tag="IVZ67_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165891.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5447..6316
                     /locus_tag="IVZ67_001817"
     CDS             5447..6316
                     /locus_tag="IVZ67_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8165892.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6297..7031
                     /locus_tag="IVZ67_001818"
     CDS             6297..7031
                     /locus_tag="IVZ67_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8165893.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7064..7972
                     /locus_tag="IVZ67_001819"
     CDS             7064..7972
                     /locus_tag="IVZ67_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8165894.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7969..8511
                     /gene="sat4"
                     /locus_tag="IVZ67_001820"
     CDS             7969..8511
                     /gene="sat4"
                     /locus_tag="IVZ67_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8165895.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8604..9398
                     /locus_tag="IVZ67_001821"
                     /note="aph(3')-IIIa"
     CDS             8604..9398
                     /locus_tag="IVZ67_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8165896.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9506..9694)
                     /locus_tag="IVZ67_001822"
                     /pseudo
     CDS             complement(9506..9694)
                     /locus_tag="IVZ67_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9674..10246
                     /locus_tag="IVZ67_001823"
     CDS             9674..10246
                     /locus_tag="IVZ67_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8165897.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10586..11440
                     /locus_tag="IVZ67_001824"
     CDS             10586..11440
                     /locus_tag="IVZ67_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8165898.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11539..11748
                     /locus_tag="IVZ67_001825"
     CDS             11539..11748
                     /locus_tag="IVZ67_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8165899.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11765..12037
                     /locus_tag="IVZ67_001826"
     CDS             11765..12037
                     /locus_tag="IVZ67_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8165900.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12039..12902
                     /locus_tag="IVZ67_001827"
     CDS             12039..12902
                     /locus_tag="IVZ67_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8165901.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVZ67_001828"
     CDS             complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVZ67_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8165902.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14027..14110)
                     /locus_tag="IVZ67_001829"
     CDS             complement(14027..14110)
                     /locus_tag="IVZ67_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8165903.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5017 a         2221 c         2926 g         4017 t
ORIGIN      
        1 ccttgaaggt gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga
       61 aagattatct aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga
      121 aactgccctt aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt
      181 tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat
      241 atattttgcg gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat
      301 tctgcgtcgt aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat
      361 ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc
      421 aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat
      481 catactatga tacgtgtttg tttttctgtg ctgtttagtg aatgattagc agaaatatag
      541 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
      601 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
      661 gaagcgaaca cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt
      721 tgtcctaata tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca
      781 taataggagg agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt
      841 ggctattttt aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc
      901 tgataagcaa tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga
      961 attttagagg ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac
     1021 agaacttaga tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat
     1081 taagcggtca atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag
     1141 gggatatagt tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag
     1201 aattgatgaa tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa
     1261 tgaatggtat tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt
     1321 ataagtacca agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg
     1381 aaattgctaa gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc
     1441 cacgtttaca acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag
     1501 aacaaactgg aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag
     1561 tagatcaaag aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg
     1621 aaaaaccaag ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg
     1681 gttattttga gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg
     1741 ggcatttagt cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac
     1801 ttgaatcttt gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa
     1861 agcagtttaa aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag
     1921 atagcgacag agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt
     1981 ctaaagataa aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa
     2041 gcggttttca aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa
     2101 cacgccaaat tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt
     2161 tacaacaaaa aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat
     2221 atctcattta tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga
     2281 ataattcata gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt
     2341 ctgcttcatc acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata
     2401 ctttgaccta atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt
     2461 gtataaagaa ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc
     2521 attttacata cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt
     2581 tgctccatgt tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat
     2641 aagcttgctt tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc
     2701 gatgaagaaa gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg
     2761 gaagaacgca atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt
     2821 aatcaggaac gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac
     2881 ctggtaacga actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat
     2941 tatgtgtcag cagataattt taatggtgac tacacggaag aactatttga agaatttgta
     3001 gtgaacatca ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc
     3061 ctgaaagaaa aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat
     3121 ggagtggtta ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac
     3181 atatcaggac tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct
     3241 gtgatggggc gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt
     3301 attgataaag agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta
     3361 ggacgagtgg tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg
     3421 aaaaaggcag ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt
     3481 ctgatagaaa gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa
     3541 aagagtagga agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg
     3601 ccgtgtttct acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta
     3661 tacagagttt ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg
     3721 tatctctggt acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat
     3781 ggacggtaac atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct
     3841 agactgcctt caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa
     3901 agagaacatc aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt
     3961 ggcacagcag gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata
     4021 ccaacaagga aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga
     4081 tggaaattta actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata
     4141 ccttgagggt cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac
     4201 agcagcggga aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa
     4261 atacatcgga gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg
     4321 agtgaaaaat gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat
     4381 tcctaaagag ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac
     4441 aggagaaggc aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt
     4501 ctgtggagat tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga
     4561 atttgtctgg cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac
     4621 cgtaaaagaa gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg
     4681 tgggaacaat atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac
     4741 agaataccaa atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa
     4801 gctggcgaac aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga
     4861 caagcgacag atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa
     4921 tgagctgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     4981 tgtaaggaag ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa
     5041 atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag
     5101 agttgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat
     5161 caaccaaatg gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag
     5221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     5281 ttggccagac tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg
     5341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     5401 atggagaaga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     5461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg
     5521 gggctcacgt gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta
     5581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     5641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     5701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     5761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     5821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     5881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     5941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     6001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     6061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     6121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     6181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     6241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     6301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     6361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     6421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     6481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     6541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     6601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     6661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     6721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     6781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     6841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     6901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     6961 atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     7021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     7081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     7141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     7201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     7261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     7321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     7381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     7441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     7501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     7561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     7621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     7681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     7741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     7801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     7861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     7921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     7981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     8041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     8101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     8161 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
     8221 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
     8281 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
     8341 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
     8401 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
     8461 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     8521 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     8581 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     8641 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     8701 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     8761 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     8821 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     8881 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     8941 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     9001 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     9061 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     9121 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     9181 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     9241 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     9301 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     9361 aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa
     9421 aagctttaac tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc
     9481 catactctga tgttttatat cttttctaaa agttcgctag atagagttct atattagaga
     9541 tgtaaagagt tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag
     9601 aagcactaac agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga
     9661 ggaggaataa aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc
     9721 aaaaaaaata attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt
     9781 ttacagagat atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa
     9841 aggatatttt cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt
     9901 gggtgcattg attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa
     9961 ggattttgat tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta
    10021 tgcacaggag ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt
    10081 ggcggataac gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat
    10141 tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact
    10201 ggggttttat gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg
    10261 aattttcgtg tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat
    10321 gatggctctt tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta
    10381 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata
    10441 aatataaaag ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat
    10501 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
    10561 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
    10621 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
    10681 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
    10741 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
    10801 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
    10861 ttcttctatt attcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
    10921 gttactgcca aaattaactc gctcatggac gtttgaaaat gaaagtagat tgcttgctac
    10981 tcttttagca cctttaaaaa gtgactatga tcttattatt attgatactg taccaacgcc
    11041 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
    11101 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttgcaaga
    11161 acagtttaac cctggactag atatgattgg ttttgttcct tatttagttg atacggacag
    11221 cgcaacgata aaatcaaacc tggaagaact gtacaaagaa cataaagagg ataatttggt
    11281 tttccgaaat attatcaagc gaagtaataa agtaagtact tggtctaaaa atggcattac
    11341 agaacacaaa ggctatgaca aaaaagtttt gtctatgtat gagaacgtat tttttgaaat
    11401 gattgagcga atcattcaat tagaaaatga aaaagaatag aatcacaaat cacaagtgat
    11461 taatcacaaa tcacttgtga tttgtgatag gtgatgataa aataaatagt aagaagaaat
    11521 agaaagaagt gagcgatcgt gggaaattta ggcgcacaga aagcaaaacg aaatgatacg
    11581 ccaatcagcg caaaaaaaga tataatgggg gataagacgg ttcgtgttcg tgccgacttg
    11641 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggat
    11701 caagccttag aagaatatat acggaaatat ttacctgaca aactttaaaa ggagtgaaaa
    11761 tgaaatggca gttacgtatg aaaaaacatt tgaaattgaa atcattaatg agttatcggc
    11821 aagtgtttat aatcgggtat taaattatgt tttgaatcat gaactagata ctaaaaatac
    11881 tcgtttacta gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt
    11941 tcaacaacca tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata
    12001 ttcaaaacaa attttgaata aagagaaagt ggcttaacat ggcgaatatt actgacttca
    12061 ccgaaaagca atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac
    12121 tagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca gggaaaacca
    12181 gtttgcgatc agcaattttt gaagaaacac aagggaatgt tattgtcatt gataatgata
    12241 cctttaaaca acagcaccca aattttgacg aattagtgaa actctatgaa aaagacgtag
    12301 taaaacacgt taccccttat tcgaatcgca tgacagaagc acttattagc cgtttaagcg
    12361 atcaaggcta taatttagtg attgaaggaa caggacgaac aacagacgtt cccattcaaa
    12421 cagccacaat gcttcaatct aaaggttatg aaacaaaaat atacgtcatg gcagtaccga
    12481 aaattgaatc atacttagga acgattgaac gatatgaaac catgtatgca gatgatccaa
    12541 tgacagctag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
    12601 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
    12661 gagtgaaact ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa
    12721 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
    12781 aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
    12841 tggaaagctt gcagctacct acaccaccaa tacccaaaac acctaaactt ccaggtattt
    12901 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
    12961 aacatttcct catgttgggg tcccgagcgc ctacgaggaa tttgtatcga taagaaatag
    13021 atttaaaaat ttcgctgttt ttttctaatt tcattttgag gtgaattaat tggtagttat
    13081 tagaggtgcc ctatacctaa taatttatct acattccctt tagtaacgtg taactttcca
    13141 aatttacaaa agcgactcat agaattattt cctcccgtta aataatagat aactattaaa
    13201 aatagacaat acttgctcat aagtaacggt acttaaattg tttactttgg cgtgtttcat
    13261 tgcttgatga aactgatttt tagtaaacag ttgacgatat tctcgattga cccattttga
    13321 aacaaagtac gtatatagct tccaatattt atctggaaca tctgtggtat ggcgggtaag
    13381 ttttattaag acactgttta cttttggttt aggatgaaag cattccgctg gcagcttaag
    13441 caattgctga atcgagactt gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa
    13501 ggtacgcttg tagaatcctt cttcaacaat cagatagatg tcagacgcat ggctttcaaa
    13561 aaccactttt ttaataattt gtgtgcttaa atggtaagga atattcccaa caattttata
    13621 cctctgtttg ttagggaatt gaaactgtag aatatcttgg tgaattaaag tgacacgagt
    13681 gttcagtttt aatttttctg acgataagtt gaatagatga ctgtctaatt caatagacgt
    13741 tacctgttta cttattttag ccagtttcgt cgttaaatgc cctttacctg ttccaatttc
    13801 gtaaacggta tcggtttctt ttaaattcaa ttgttttatt atttggttga gtactttttc
    13861 actcgttaaa aagttttgag aatattttat atttttgttc atgtaatcac tcctgaagtg
    13921 attacatcta taaataaata cagaagttaa acgatttgtt tgtaatttta gttatctgtt
    13981 taaaaagtca taagattagt cactggtagg aattaatcta acgtatttat ttatctgcgt
    14041 aatcactgtt tttagtctgt ttcaaaacgg tagatgtttt atctacatta cgcatttgga
    14101 ataccaacat gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc
    14161 aattcctatt atacaaaatt t
//
LOCUS       DACCZY010000073        13895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCZY010000073 DACCZY010000000
VERSION     DACCZY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.074"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVZ67_001830"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IVZ67_001830"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8165904.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IVZ67_001831"
     CDS             complement(1174..1869)
                     /locus_tag="IVZ67_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8165905.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IVZ67_001832"
     CDS             complement(1882..2598)
                     /locus_tag="IVZ67_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8165906.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IVZ67_001833"
     CDS             2928..3536
                     /locus_tag="IVZ67_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8165907.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IVZ67_001834"
     CDS             3861..4184
                     /locus_tag="IVZ67_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8165908.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IVZ67_001835"
     CDS             4366..5736
                     /locus_tag="IVZ67_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8165909.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IVZ67_001836"
     CDS             complement(5866..7167)
                     /locus_tag="IVZ67_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8165910.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IVZ67_001837"
     CDS             complement(7521..9512)
                     /locus_tag="IVZ67_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8165911.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IVZ67_001838"
     CDS             complement(9656..9922)
                     /locus_tag="IVZ67_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8165912.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVZ67_001839"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IVZ67_001839"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8165913.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVZ67_001840"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IVZ67_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8165914.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="IVZ67_001841"
     CDS             13266..13829
                     /locus_tag="IVZ67_001841"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8165915.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
BASE COUNT         3987 a         2901 c         2564 g         4443 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaa
//
LOCUS       DACCZY010000074        13872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCZY010000074 DACCZY010000000
VERSION     DACCZY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.075"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            142..1593
                     /locus_tag="IVZ67_001842"
     CDS             142..1593
                     /locus_tag="IVZ67_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8165916.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1942..2574
                     /locus_tag="IVZ67_001843"
     CDS             1942..2574
                     /locus_tag="IVZ67_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8165917.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3162..4601
                     /locus_tag="IVZ67_001844"
     CDS             3162..4601
                     /locus_tag="IVZ67_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8165918.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4896..5114
                     /locus_tag="IVZ67_001845"
                     /pseudo
     CDS             4896..5114
                     /locus_tag="IVZ67_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5363..5578
                     /locus_tag="IVZ67_001846"
     CDS             5363..5578
                     /locus_tag="IVZ67_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165919.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5795..6051)
                     /locus_tag="IVZ67_001847"
                     /pseudo
     CDS             complement(5795..6051)
                     /locus_tag="IVZ67_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6238..7149)
                     /locus_tag="IVZ67_001848"
     CDS             complement(6238..7149)
                     /locus_tag="IVZ67_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8165920.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7168..8172)
                     /locus_tag="IVZ67_001849"
     CDS             complement(7168..8172)
                     /locus_tag="IVZ67_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8165921.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8169..10292)
                     /locus_tag="IVZ67_001850"
     CDS             complement(8169..10292)
                     /locus_tag="IVZ67_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8165922.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10297..12744)
                     /locus_tag="IVZ67_001851"
     CDS             complement(10297..12744)
                     /locus_tag="IVZ67_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8165923.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12731..13120)
                     /locus_tag="IVZ67_001852"
     CDS             complement(12731..13120)
                     /locus_tag="IVZ67_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8165924.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13180..13683)
                     /locus_tag="IVZ67_001853"
     CDS             complement(13180..13683)
                     /locus_tag="IVZ67_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8165925.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13696..>13872)
                     /locus_tag="IVZ67_001854"
     CDS             complement(13696..>13872)
                     /locus_tag="IVZ67_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165926.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4290 a         2725 c         2526 g         4331 t
ORIGIN      
        1 gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa gaaatatact
       61 gtattactca attcattgaa aatatttatc ataattttta atagaaaaca ctattaaaga
      121 gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc agttatatta
      181 atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa ttcaacatac
      241 aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt aacagcagag
      301 agtagtgaac ttttgacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
      361 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca
      421 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt
      481 agtacgattt caagtaccga atcttcaaca acttcaagta gtacaagtga aataacttct
      541 tcgaaagtga caaaaccaaa ccatatagaa tcttcaaaaa aggaagcgcc aaaaacggaa
      601 ggacaaacaa aaactttttc gacaccacag caaatagaat caacagaatc tttccaatca
      661 caaccaatac tatctggtaa tgaagaaatt cattttgaca aaaatgaatc tgtagaaaat
      721 tttattatga aaattggtga gtctgcgaga aaaattggtc agaaatatga tttatatgct
      781 tctgttatga ttgcacaagc gatcttagaa tctgccagtg gtcaaagcca gttagctcag
      841 gcaccgaatt acaatttatt tgggataaaa ggaacttata atggaaactt tgtcattatg
      901 gtaacaaacg aagatttggg taatggaacg ctttatacga cccaatcaaa atttagagtg
      961 tatgaaaact atgaggaaag ctttgaagat tatgccaaat tattaactaa gggaattagt
     1021 ggaaataaag atttttatgc aggagcatta aaagctaata gtaaaacata tcgagaagca
     1081 actaaattcc ttacaggacg gtatgcaaca gatacacaat actatttgaa gctaaatgaa
     1141 ttaattaaaa cctacgattt aaccaactat gataaagaag ttaaaggtcc acaaatgagt
     1201 aagactggat accaagtgcc tattcataat tattcaataa ccagtttgtt tggaaatcgt
     1261 ggaaatgaat ttcaccgagg acttgatatg gcagctacac agggtgagcc gattcatgca
     1321 gttaaagctg gtaaggttgt taaagcagag tttcactact cttggggaaa ttacgtggtg
     1381 atacaacacg aagatggatc aaccgcgtta tatgctcacc agcaacaata cctagtaaaa
     1441 actggagata atgttttgca aggacaaata attggctatg taggaagtac tgggaatagt
     1501 actggcccac accttcattt ggaaatttgc aaagatagta gtctttctca gagcatgtta
     1561 gttgatccga aaaccttatt attcggagac taaaaaaggt aatcaatctg acaaagtaac
     1621 aagaaaatgg ttggattctt agacctttga tcaggatatt gagactggaa ttgatatctc
     1681 gattacgatg ggtttgacaa ataaaacaag tccattatcg aatatcgagt gtattattgc
     1741 cgtctttatg ctcatatttt gagcaggttt cactagatga aaacaattta tctatcttga
     1801 ggatttcgtg tacgtatcgt tcagtcttgt attgtaaatt agtcataaaa tcagactaag
     1861 aaaaattgga aatatttttt tctgatttat taaaagttaa ttgaaattat atggtacttc
     1921 tttcttggga gggtaggtgc agtgaaggaa agatatggtc accttactgt tgttgaggag
     1981 attactcata atggatataa aaaatatcgt tgcctatgtg actgtgggaa tgaaacgatt
     2041 gtatatcgat caaacttact atcaggtaga accaaaagtt gtggatgtgg aaattataaa
     2101 aataggcata aatataaaaa tttgacaaat aaaacttttg gtaagttagt tgcaatcaag
     2161 ccaactaaac tgcgaagtga aggaactgtt gtttgggaat gtcaatgtct gtgtgggaga
     2221 aaaaagttag tatcggcaaa gcgacttcta aaaggtgatg taaaaagctg tggatgtttg
     2281 aaatcaaaaa agcatcacat tgaaaataaa aaattcggat tacttactgc aaagtatcct
     2341 ttggacaata actgttcatg gaaaacaaaa tgggtttgtc attgtgagtg tggcagtatg
     2401 tgtgagataa gttatagtaa cttatttttt ggacatactc aaagttgtgg atgtttaaaa
     2461 aggcaagaaa caaggacatg tgttgaaggg acagtaatag agtgtctaac gtcaaaatta
     2521 ccgaaaaata atacaagtgg tgttaaggga gtttatcgtt ctaaagagaa atagattgcg
     2581 tatataactt taaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca
     2641 gctcaagcta gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga
     2701 ggtaatcatt aattattgtg gataataatt aacagtgata tataaacatc tataatatat
     2761 tattcatact attgatattt agtataatcc tatggaattg aatatcatag caatgtattt
     2821 gaaaagcaaa ttggaaaaca aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa
     2881 gtagaatgga aatttgctta cgtctcacga gatagctcac actattaatc tgaagaacaa
     2941 tgaagtcgac ttttagaaat tgctagaaga aagtaaagcg aattatcact agttactata
     3001 gagtaagttc aaataaagca tctccaactt acatggttac atgaggatat caaatcgaaa
     3061 ttactcaagc ttcctatggc tagagtcgcg agaagttgtt gggcagtaga cataggttat
     3121 attgttctta gctacacgat cattagaaag gaaatcctat tatgtatagt tatcaagaag
     3181 atgcaaaagt ccaaaaaaat cgctggtgga ttttgattgc tatatctatg tttaccttca
     3241 tgtccacatt ggatggcagt attgtcaata ttgcactacc taccatctca gatgatatgg
     3301 gcataccaat gaatcaagcg gaatgggtag tgtccgttta tctgatgatt gtctgttctt
     3361 gtttgttgct gtttggtaaa attggcgata gccgtgggaa aatcaaagta tttcggattg
     3421 gtacgctgat ctttgtcttg ggatcgctgt tgtgtgggtt caatcattcg ctaggtattt
     3481 tattgttcgc acgggtggta caagccattg gtgctagtat gactatggct acaaatgccg
     3541 gaatcatcac ggaagtcttt cccatacatg aacgtggtcg ggcattggga gcaataggtg
     3601 cgtttgtttc attaggctcg attgctggac caggtcttgg cggcttgatt ttgtcccaat
     3661 tttcttgggc gtacattttt tggatcaatg taccggtggg aatcgtaacg atgttgattg
     3721 ctgaaaaatt cttaccaaaa gatatcacca tgagcggcca tacaatcgat ggagggggct
     3781 ttgctgccat tgcggctacg attatcagct tttttggagc aatctttttg gggcaagaaa
     3841 gtggcttttt ccatccagtc ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca
     3901 gctttttaaa agttgaaaag aaagcagcct ctccattgat ttctttcggt atttttaaga
     3961 acaagatttt taccatgagc ttgattacag ccttgttgat tttttcttcc aacttctttg
     4021 tcaatgtagt gattcctttt tatctgcaaa atgctcgcgg gttgagtcct agctatgctg
     4081 ggctgttgat gatggtcttt cctttgatga tggtcgtcgg ggcgccttta agcggctacc
     4141 tgaccgaccg ggttggcccg gagattttgg tattagtagg cttgggctta ttgtcggtaa
     4201 cccaggtcat gtatatgttt ttgtcagtgg actctccttt gtggctatac attgtcgcta
     4261 ctgccatcat gggtgccggc aatgccttgt tccagtcccc caataatacg atggttatga
     4321 gctcggtcac tatcgaaaac ttgggactgg caggctcgat gaattccttt gcccgaaatt
     4381 tggggatggt cagcgggatt tcgctttcta cgacggtctt atatcaagcg atgtctgcta
     4441 agatgggcca gcaagtaact aactatatcg caaatcggcc ggatgtcttt ttatatggaa
     4501 tgaagattac ctttttaggc agctttgttt tgtgttttgt tgcctttttc ctgactatct
     4561 ggcgtatcaa aaaacaagga ggtattcaga aaaatgtctg aaaaaaccac tgttgaagaa
     4621 tttttggagg cctgcgtatg ggtgagtggg atttccatgg atgatggaag atgaagtgaa
     4681 cttctaaaag ccgaaaaact caagtattat cttcaaaata ctagtgctca atgagatgct
     4741 tgatttgatc acaagaagac tgcgaaagcc gctggcaacc gcaagctagg tatcccaccg
     4801 atggactacg ttttagaggc tactggttat gaatacggag ccaacgctct catctgcata
     4861 agaatacatc acccagatta tgtaaagatt ttcgatactt ctgtcaaaaa atcattaaga
     4921 tagataaatg atttccgtct agtcaaattg gttcagaatg tagacacaca gatggtaaga
     4981 agtcactcac aattcgagaa tggacttgtc ctatttgccg tatacatcat aatcgaaacg
     5041 tcaacgatag tatcaatatt ttgaccaagg gctatgaata caagcattgc cttagaaaac
     5101 atcaagaacc ttaggaacaa cggggatagc ttggttaata agagataccg ctgtttaggc
     5161 ttgcaagtat actcttttat caagaattcc gtggctttag tcatgagagg cttaatacta
     5221 tgaaaatttt ctttagcatt tttgatttag gttccttttc taattataat aagatgacca
     5281 ctacagtaac ctactgaaat atataaacag ccactgtagt accattttca aagtagtcta
     5341 tgagaggaga atcttcattc ttatgaacgc tggaattgcg gatatgaatt taataaaaaa
     5401 aacccttaat gattttactt ccaattcaat atcaaaaggt actggtatca atttaagcac
     5461 aataaaaaag ctgaaaagtg gtgaaagatc agtggaaaag ctgaatcttt tagatgctat
     5521 aaaaataacg gaatttgcta tgaaaaatgg aaaagctgag attgaaatat ggagataatt
     5581 atcgtctagt gaattacttg ccacttagct gaatttaacg atccttggtg cttgaagggg
     5641 gagattcttg ggaatagagc atacttgcaa gcctatgtct ttactaacaa cgatatctct
     5701 tattaaccaa gctatccccg tagttctaag gcttttactt ttatctaagc aataatcgta
     5761 ttcaaaggaa atttttgacc cttagaaagt ctcattttat cagacgtgag cttaggcatt
     5821 gctgaatttt gttaattcgg ttttaggttg tgctttgagc ataatatgaa agtattcttt
     5881 atcacgattt cattcatcta aggtaatgtg atgtgattct gaaattcttt caaaggcagt
     5941 ttttgcataa gctgacatta catcatcaat gacatgttta caatgtttca ctactaaaat
     6001 aagggggtaa ccaagaagaa aaactgaatg attatttgta tctaattcca tggtatatca
     6061 accatttcct atatagactg attatgacat ggattaaaat aaaaaggcac tatgtcttaa
     6121 ttgtcggttt tcgagttacc gaatggcatt catcacccac ctatagagga tgggcgactt
     6181 ctgcctgaaa taagttacac tacttataac ttctaataaa attagattga gtcattatta
     6241 tttaacatca acgattttcc agtttccatt tttttgtaat actaatttgt actgaaagta
     6301 gctagtggtt tttgatacat tatcaaggta ttttatcttt gtactcacta gaaaattttt
     6361 tccgttttgt agataaactg gattctctaa ctccgaaaac tttaagtcaa tattgattgg
     6421 tcgcatcgca ccattttcaa cataatactc caattctttt tcagaagctg atggataaag
     6481 tttaaaaaat gtttctaaaa aatcggtgat ttcttccgtt gttttggcat caatttctga
     6541 atcgttttct acttgttttg cttcatattt tgccttgtct ggctttccaa tcattgttgg
     6601 caaactagtt actaaatgat tcctgttttc atcttcaaag attgtcactc gatatgcaga
     6661 agaaattgat tgcgtctttt ttccctctgt aatatgttgc tccactaaaa atgaaacgac
     6721 aaattcttcc gagtgttttt caacatctag aatttttaca gattgaactt cggaactcgt
     6781 gggaatatct gctcgaacca tatcttggat taaagcgaga ccgttttctg taaggtactg
     6841 ttcgagatta ctcattctct tttcaattgc ttctttgtta tttttccatg aaaaatattc
     6901 tttcgcaaaa tcctctgtaa aactttcaac gccagaagta ttcactacct tctcttttat
     6961 aaccactttt tcatgagttg tatgttgatc aatggcggtg aaatttttat aaacgccaaa
     7021 agcaagacta caagacaata aaagccaaag ggcaagcacc atttttcgat gcttacccac
     7081 ggatacaact cgttcttttt tcttttttgg gctcttttcc ttttgattcc gttcaatttt
     7141 tatcttcatt ctttttcctc ttttctctta tttgtatcgg ccagctccga taagatgctg
     7201 ccgccaatag ttttccgtta gattcgtcca ccccactgga tctcctgcat gaaacatgcg
     7261 attctcccct gcgtaaatgc ccacatgggt cacataagta ccggcatcat aggttgaatg
     7321 aaagaaaact aaatcacctg gtttggcatc ttttagatca atatgagtca tggcatcata
     7381 ttgatcctga gccacccgag gaagtttcag gtcaactttg gcataacacc attgggtcaa
     7441 gccactacaa tcaaaccccg tttctggact tgagccacca aacacatagg ccgttccttc
     7501 atacttaaat gcctcatcaa aaatttcttg gactattttg tcatcaaact gagttgtagt
     7561 taggtattgc ttcactaata aacagtaaaa catattgcca taattatacc gccacccacc
     7621 attttcctta atcgcgattg ggttggggta atcgactttt tgaccacctg atttttcctt
     7681 tgaaaaagat tcagctaaag caaaactatg cttcttcccg tttttcccga catgatctag
     7741 atacccgcca ccatagttat aggcctgaat aatactatct tgatcacacc ctagttcttt
     7801 ggaacgctta accaaagacg caaaatagag acagccttgt ttgatggatg cctcggtact
     7861 taatgaatta ggtggtaatc cggctgactc agaactttgc ataacgtctt ccattgtgcc
     7921 accagattcc acttgaataa tcgccaaaag aattggaata tactctgtta tatccgattc
     7981 tttggcatac ttttccacca ttgcacggtg tttcaatact tcttctgaga cactagttcc
     8041 gccaggagaa gtgccagaac taccatttcc ctcactgtct tcaccaaaca acaaacccac
     8101 acacatcaaa atacccataa aagagatggt cagaaaagtt aatgaaataa cacccacttt
     8161 cttcaagttc attttttctc acctgacctt gattttggac gatggttcat tcttttttgt
     8221 actttttgat tggtctgctt tggcagagtg gacctctgtt tcacttgttg gaaagaattt
     8281 ttcttacttg ctgattggct tttccctttt gaaatgattt cttgactagt tagttttcgt
     8341 ttgatttcgg gattcagatt tgctctcaca atccgctttg gtgcttcttt tttcagcatc
     8401 atatctttct gaggttgagc tgctttatct attggaatat tcgcccttct ttttccctga
     8461 cgatttgttg cttcactcag tatcttgcgt ttttcgtcca ttttcgcatt tcttcgttgt
     8521 cgttgttttt ctcgcaaatc cactcgatcc gctttttctt ggaacagtcc gcgcttgaat
     8581 tcttctggtg cttttttcga cttttcgatt cctttatgga gattgtattt caaattcgtt
     8641 ggcgtattcc ttacctgatc ctttacttgt ttagtcttat ctttcaccaa agcttgggta
     8701 tccaaaactt tgccgatttt tctcccagcc aaattcattc gggattgttt cttattattt
     8761 gaaactggat tctccttagt taagtcagat agtatttcgt gatcgttcgg taatcgtgaa
     8821 ttctttcgta gagaagaacc ggatggctta aatttggatt ttgattgttc ctttgccaac
     8881 ttatttccga ctgctgcacc accaccaaaa gccaaagctc gtcccacatt tcgattgagt
     8941 tgccgcaatt ttcgattcat taacatttgc ggttttcgca tgacgcgtct tcccattgac
     9001 tggctatcat tactctggag gttgaacata ctcataatgt cgcccaattt gaaatagata
     9061 ccggcaaagg tcacaatttg cagaaaagca accatgaaaa atggataggt tgccgaaata
     9121 ttgaaaaaca tcgtggagat gctaaatgca gtcgtaatca ccaaagtaac acccgctcgc
     9181 atcataatcg tattgaataa acgaataatc gccttctttc ctaaactttc ataagtcggc
     9241 aacatggaca ataagaaact aattggcaga aacatcgcaa agataatgaa gagaatttgc
     9301 gagaacaaca taatccctgt tagaagaaaa acaaagaagg aaatgccaat attgaacaat
     9361 accaaaaaga ccacggtccc taaacggttc atggtcttgg taatactgag attcgcattg
     9421 tcattgtctt caatctctgc cttgaccgct tcttcccgat ctttgccttt gttttcatct
     9481 ggactcacag atagaatctt gttgacccgg tcttcaccaa tttcttctat gttggaatca
     9541 tcaaattgca gtagtaacca aggttgttgg acttgaatcg aaaacaaact atcccgaatc
     9601 aaatccacac tatcttttcc ttggctttct gaattgggta caacgatctc ggttccaagg
     9661 gtaagggccg cttcactaac atctgagcta aagtcattga tttttgtgat ataggtaggc
     9721 gcataagcaa tgaacgaacc cgatagtaga aaaatcatca caaaattaat cacggctcga
     9781 actgctttcg tggtttctcg ttttaataat ccggtatacg ccacatacac ccccagagct
     9841 aaaatcatca ataatagaaa cccaaaataa aatccggagg tctggagtcc attttcagta
     9901 ataccagcca atgtttggat attctttcca atactttcag ccgtatccga aatgaaatct
     9961 aatttgtaag cctcttgaac cacataaccc gtggcgttcg acaaatacaa actcaccgtc
    10021 caaatgaaat tagtaatggc ataaagacca tacatcacgc ttttccctaa gccatcaccc
    10081 caattccaag gcaaccaatc ccaagagcta tccacaaaga aatctagttg atagttgttc
    10141 agtgaatatt tggaatatag atttcctgct tcaactgtat catcaactag ccctgtcgcc
    10201 tcggctaccg taccaactaa actcaataga actatcagag tgagtatgct aataaaaacg
    10261 attcccacaa tccgaaaaag tttctttttc atcccctcac tccattctct cttgaacagg
    10321 tggacgggta tcaaaggcat ggaagagttc ttcaaagact ggatgtacct gaaccacgcc
    10381 tacccgacca tacaaatcct gcatcaagca ttggccattt tccaaatctc gtaggcgttt
    10441 ttgattgctt tcatcttctc gatccaagcc aaagaattcc aaggttttct tgatttcatg
    10501 aatatcggta ctgcgaaacg caaacttcaa cccgatatta ttcttcaagc gttcatccag
    10561 taaatcatca ctattttgcg tgacaaagta aaccccggca ttcatagccc gcccagcacg
    10621 aacgagctta ttcgataatg ttttcccttg cgctacttga agaaaactcc acgcctcatc
    10681 taagtctaca attttgaaga tttctcgatt actatggata aagtccaagg caaaagtgga
    10741 aatgacgatt agcatggaaa cactaagcaa ttccatggtg gtatattctt caaaagtggt
    10801 ttctgcatct ggtaaaacca aatccgctac ttgaatgata tttaactgcc tatctaaact
    10861 aatcgactgg gtaatcgaac catctgagaa aagcaaatgg gcaaaatcat aatcggtaaa
    10921 ggactcgatg tgatccgcga tattttccgc aactggtgag ccttcttttc gaagctcatt
    10981 aattacttgt aataatcccc gttgattgct ttgggtaacc gtccgaatgg ccttacgcaa
    11041 cactggaaac ttctccccat ctcgactaga aattcctgtt aaaaaggtca gaatatcaat
    11101 tgctagactt tcagaatcct tctttttctc cataatcaca taaggatcaa gtaatccttg
    11161 attttttggc ttactagtta agttgagaat attgatttca tccgcaattt ctggcaaggt
    11221 ttctttccag ccaccccgtt ccccttttgg atcgacaatc acagcttgtc caccaaaaag
    11281 gacggcgtaa taaactaaga gattgttact aaaggacttt ccgccaccga gtgaaccgag
    11341 gaatgccgct gctagcgcat tggtaactga atccttgacc ccttgtgagg ccaatgccgg
    11401 tttcaggtaa acattgcgcc ccgtatctac gttataacca aagtaaatgc cttccaactc
    11461 ccctaacatc tgagtggcgc caaaaccaag tccggcgaga aaatcagacg taacatactg
    11521 gatataatca ttcatgtatc gcttactaga aggtaagaat tcttcatgta gccccatcat
    11581 atcgccaaaa ggccggacta gttttacatt ggtatcatca taaaaatcta gcacttcatc
    11641 acaccggcgc tttagttcat ctactgattc ggcacttact cgaaccacat agctcagttt
    11701 gtacatggat tctttggact gatccaaggt agtttccaat tcatctacgg aatccaatgc
    11761 atctaagaca ttggaatttg tttcattgtc agattgatag gcatggttgt ccaagtcttt
    11821 taattccttt ttcttattgc gaacggtagc aagtgccttt ttgttcgtca caatctccac
    11881 attcatacta gtgtcaattg ggaatgtaaa ttgttgttgc tggtaataga aaagttctga
    11941 ggagggaaac tccatttccc ccacaattgt attaattgtt aagtacgcta catatgattc
    12001 atgattctca tgttccatgc ataagtaccg gggcttttct tcaatcaaac aacgactggg
    12061 acgtaataag tcgtaacgtt tcaccaaagt ttccgacttc agctttttct ttggtagctg
    12121 aaattcatat tcttcaaatg gtgtaccctt ttcgccatag atatgttcaa tcaaatacat
    12181 cagatcactc ggtgtgactc gtctgacttt aaatcttcga gccaatttac tttccattag
    12241 tttttcaagc ttggtatatc ggcgaatttc ttcattggat aaggaaacaa aatcgcccat
    12301 caaatgatga ttcactccat agacaaattc ttgaaagccg gagaagaaag atttcttgag
    12361 attctttaga ttgacctctt catctgtcgc aatcagtttg aagccaataa aaaagcgata
    12421 atcaatctga ctatcgccaa tcatggatac taatgcctct gtctgtaaat caattcgttg
    12481 tttcgctact tctgttaatc gcccggtaat ttcctttttt gatcgttctt gcgtggcacg
    12541 aatactactt tctgtggcaa tttgtaaggc atgaatcttg ccttcacgat tttgcgcaat
    12601 caattgacga aagttatcgt ggacttgata tttctgttca ggtgacaaaa aggagtaatt
    12661 gtaaggtatt aactcatagt aggcaaaaca ttctccctct tgattaaaga caagattatt
    12721 ttcaatatac ttaatcgggt acatagttca cactccttac cagagtaatg ggttcgtccc
    12781 atcgtttctt ctgttctttg actttctttc ctgcgaaagt gaccttaaat cgtaagtgat
    12841 agctaataaa agatcgtaaa aatcccaatg gtttcttacc gtcaaacgtt ttttgcgaca
    12901 tgaaccaagt aactgccacc ggtataccta agtattttaa aaaggccccc tctatcatag
    12961 atagtggagg cacatgggca aatacgatga caaacagcaa ggataaaaca aaccaagcca
    13021 tctggttaaa agtcacagga aaaggcagtt gaaagtcatt aattgcataa atgacctttt
    13081 caactgccca aatactggtg tagcttttta ttttcttcat caaatcattc ctttaaaaag
    13141 aaggatagtt tagaaatcaa ctacccttca ataattttgt taatatggat actcaaaaac
    13201 acctcgactc gttaccaaat aattgcctcc aatctccaag tctctaccac acgcatcaaa
    13261 atcaatatac gtcttcaagt gttctggtac ttctccaaaa tcaccagctt ctacaatcaa
    13321 gaaatatgct acatcactca tatcatcgca atctggataa tagataatat catccacatg
    13381 ctctaccatc tcctcaaaag aattgaagaa agcatgttga atctcagaag caacttcacc
    13441 aactggcgtt ccttcaagtt cctccgctaa gccacacaac cgattaattt cctcaattgg
    13501 cgtatactca tcaatctcaa agggaagttc ataatcatga attgcatatt cctcgtattc
    13561 gtcatttaac ccaattcgtt ctttcacttc atcaaaatcg acaggtgggg taaaccacgc
    13621 ccccaccaat tcaccttcgt tatatttccc gaggttcgca atataaacac gcatctgttc
    13681 catcgtgctt cccccttacg caccgacaat tcggttaaac aaactgagta agacatcctt
    13741 caccccagaa gcattaaaga ccaatccgac cgcaatcaaa gcaatcacca aaaagccaat
    13801 cagtttggaa aactcccgct taaatcctaa aaacaaacca atgaccacaa ttgccagtaa
    13861 gactaaggat tg
//
LOCUS       DACCZY010000075        13739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCZY010000075 DACCZY010000000
VERSION     DACCZY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.076"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..259)
                     /locus_tag="IVZ67_001855"
     CDS             complement(<1..259)
                     /locus_tag="IVZ67_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8165927.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDR"
     gene            complement(398..1213)
                     /locus_tag="IVZ67_001856"
     CDS             complement(398..1213)
                     /locus_tag="IVZ67_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP8165928.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            complement(1210..1527)
                     /locus_tag="IVZ67_001857"
     CDS             complement(1210..1527)
                     /locus_tag="IVZ67_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165929.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            complement(1527..1817)
                     /locus_tag="IVZ67_001858"
     CDS             complement(1527..1817)
                     /locus_tag="IVZ67_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165930.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            complement(1811..2116)
                     /locus_tag="IVZ67_001859"
     CDS             complement(1811..2116)
                     /locus_tag="IVZ67_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165931.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKKC"
     gene            complement(2119..2280)
                     /locus_tag="IVZ67_001860"
     CDS             complement(2119..2280)
                     /locus_tag="IVZ67_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8165932.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2295..2654)
                     /locus_tag="IVZ67_001861"
     CDS             complement(2295..2654)
                     /locus_tag="IVZ67_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8165933.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(2651..3502)
                     /locus_tag="IVZ67_001862"
     CDS             complement(2651..3502)
                     /locus_tag="IVZ67_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8165934.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(3519..4349)
                     /locus_tag="IVZ67_001863"
     CDS             complement(3519..4349)
                     /locus_tag="IVZ67_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8165935.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(4352..5038)
                     /locus_tag="IVZ67_001864"
     CDS             complement(4352..5038)
                     /locus_tag="IVZ67_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165936.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(5044..5283)
                     /locus_tag="IVZ67_001865"
     CDS             complement(5044..5283)
                     /locus_tag="IVZ67_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165937.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(5609..5797)
                     /locus_tag="IVZ67_001866"
     CDS             complement(5609..5797)
                     /locus_tag="IVZ67_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165938.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(5818..6102)
                     /locus_tag="IVZ67_001867"
     CDS             complement(5818..6102)
                     /locus_tag="IVZ67_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165939.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(6104..6298)
                     /locus_tag="IVZ67_001868"
     CDS             complement(6104..6298)
                     /locus_tag="IVZ67_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165940.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(6295..7071)
                     /locus_tag="IVZ67_001869"
     CDS             complement(6295..7071)
                     /locus_tag="IVZ67_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8165941.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(7198..7419)
                     /locus_tag="IVZ67_001870"
     CDS             complement(7198..7419)
                     /locus_tag="IVZ67_001870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165942.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            7720..8064
                     /locus_tag="IVZ67_001871"
     CDS             7720..8064
                     /locus_tag="IVZ67_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165943.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8100..8495
                     /locus_tag="IVZ67_001872"
     CDS             8100..8495
                     /locus_tag="IVZ67_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8165944.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            8548..8859
                     /locus_tag="IVZ67_001873"
     CDS             8548..8859
                     /locus_tag="IVZ67_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165945.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            8871..10019
                     /locus_tag="IVZ67_001874"
     CDS             8871..10019
                     /locus_tag="IVZ67_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8165946.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(10112..10405)
                     /gene="rpmA"
                     /locus_tag="IVZ67_001875"
     CDS             complement(10112..10405)
                     /gene="rpmA"
                     /locus_tag="IVZ67_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8165947.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(10418..10762)
                     /locus_tag="IVZ67_001876"
     CDS             complement(10418..10762)
                     /locus_tag="IVZ67_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8165948.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(10789..11097)
                     /gene="rplU"
                     /locus_tag="IVZ67_001877"
     CDS             complement(10789..11097)
                     /gene="rplU"
                     /locus_tag="IVZ67_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8165949.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11379..12020
                     /locus_tag="IVZ67_001878"
     CDS             11379..12020
                     /locus_tag="IVZ67_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8165950.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(12082..13713)
                     /locus_tag="IVZ67_001879"
     CDS             complement(12082..13713)
                     /locus_tag="IVZ67_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8165951.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         4038 a         2779 c         2233 g         4689 t
ORIGIN      
        1 aacgatcttg atgaagtttc ttgctccaat ttgtttgccg atccagctca cgcatctgat
       61 cgaacccttt ttgaatttcg ttgtaataga attcaatgtt tgctgctgct ttccaatgcc
      121 tgctacttcg cactcctgct cctgtttcag ccatttccaa cttaactaat tccgctcgtt
      181 cttttgattt tttatctttc tgaatcttca tcatgatttt cttgaggatg atttcactgt
      241 attgtgtaat gagatccatt atttctcctc ctaaaatttc atttcatcgt cgttgtcatc
      301 ttctttgtca tcagttttgg aaaggagggc atacacgaga tatgccactc ctaccaaacc
      361 taagaacaag agaattttag ctataaagaa tcccatatta tttacctgtt tcatccgtaa
      421 ctacagtatc tgctccgttt actgttaccc atccatgctc ttttcgagct tgagcttctt
      481 cataacgaat taaattatct gttacagatt cggcaacttt acggtttgat tcagcttccg
      541 cttctgcagc ttttgttttc ttgtaagctt cactatcagc ttgagttttt gcagtttctg
      601 catctagctt cgctttttca ttttcttgac cagctcgaat gatcgcatca attgattttt
      661 gtgtttcttt atcgacatct ggaacaccaa gtgttacgtc ttcgacttca aaccctttag
      721 attcaactga tttagcaaag ttcgttagta cctcagcttc aactttagag gaatctcctg
      781 aaaggacatc aagcaggcta tatttggcat aaacttcacg cgctactttt tgaagcttag
      841 attttaacca tccactttcg atatcttccg aagttatatt cccaaattct ttgtacattt
      901 ttgctgcttt agttgaatca actttgtagt catatttgat atcaatcgtt gtctttttgc
      961 catcgcttgt tgatactgaa atgttttttg attggatagt ttgcaagcga attggatatt
     1021 ggatcacttt gtcaatccca acaaatttca caccttgcgt cagtgcttca tctttgatac
     1081 cgccattcat tgaatagcgc acacccacat atccgttatc aattttttcg aaaaacttaa
     1141 atcctccgat aactccaata cctactatta ctactcctgc cacacctagt ttgattaatt
     1201 tattttcgtt catttttctt cctccagttt ttttatttct ccaaatgaca attcaccggt
     1261 tccattcgct tctacaggta cgatatattt attcatatcc tgcttctcca atcctgaatc
     1321 tttgaggatc tcgtttgtaa gattgacaat ctccataagc tgctctgttg catccaagtc
     1381 ttcttgcgtc agaagattag cgccgcattt cggacatggc ttgttcaacc attgcggata
     1441 attttcgtat tttactgcca tatcagaata atcacattct ggattatcgc atttgatccc
     1501 acgtacattt aagtctacga attccattat ttctcctcct caatctcaca tgcctgttca
     1561 aactgtctag tgatgttttc taacgctttt ttgtactcga taatactttt tatcgttctt
     1621 tcttcactta acacgtaatc gcgttgtatc gccttcaaac acgatgagac agtttggaag
     1681 tatccgatat ctgctcgtga ttcttctttt gcttctgtgt agcggatatt tccttcctca
     1741 tctcgtctta cctttgatag gacaatgttt ctagaatcac tggtaattcg ataatcttcg
     1801 attctcatgt ctagcatttt ttctcctcca catacctaaa ctgtcgtcct tttgaatcaa
     1861 tccataagct cctagctcta tcccagataa tgtttttgct taatccagta atttcagata
     1921 actgttcagc tgtacctgtt actagaattc gatcaccatg ccagattgca atttttctcg
     1981 gcgttttccg tttaggcttt tcagtccaca ttgatttacc gagcttttgg acttctgcaa
     2041 ctatttcttt gtcttcttgc caagattctg acttggttaa ttcaacaatt cgtttcattg
     2101 ttgctttctt atccacgttt attcctccaa tctacgaatt tcccttctta aattctctat
     2161 gtgcaaatcg attgcctttc tcgccgtttc attgaccatc actgcctttg ttcgttctag
     2221 atcgtcaatt tcacgctgaa tgtttcgaat acgcatttga atcacttctt ctgttgtcat
     2281 gatggaccac ctcgttaaaa acgctcttcc ttgaacgtat tccgatattt tttagctaat
     2341 atcaacggcg cttgatattg atgacagaac aactttgcct tgatcttaaa gtcttttgtc
     2401 tgcattcctt tgacatctac gactttgaca agtttgccgt ttttataaaa tgtgaagtcg
     2461 ggaatatact cgatcttgcg atacttcttt ccgtctagtt caaatttcgg catcagctca
     2521 aatctttcct gaagttttac tttccagcca tttgcttcag cttgccacaa ggctagatcg
     2581 taatactctg cttctgcgat agaatcaaac ttgatacctc gatgaacagt ttttttatta
     2641 cggtatttat tcatgcgata ctacctttca ctggttttat gcgcttgtct gctgtttgtt
     2701 ggaatttcag cgcataacct tctgaattct taaatatcct agaaacaatt ctttcaccgt
     2761 aggcttctct tagttcagga ccagataagt ttgttgtgat gatcgttgcc ttgttctgtc
     2821 tggcttctaa gagcgtgttt aacgtgttgt ttgtaaactg cctactattt gataccccac
     2881 tacctaattc agctccaata tcgtcaaaaa ccaccaaatc agttgtttta atatcggcta
     2941 taagcgatcc ttcaatttct tttctcagtt cagtattgtt ataagaaaac tttatttgct
     3001 ctaataattc ttgatagctt ataaaaagta ttttcttgtc ataatttgag cgctcaagta
     3061 tttcccaagc tgtggccatt gataagtggc tttttccgct tcctgatttc cctgatagaa
     3121 tgaaatgtgc aggatggttc agcagaacct cattcgtata gctttttgct ttttctaaag
     3181 cgatttttgt ttcttgatcc actacgtgat aattttccat tttgcattta aacaaagttt
     3241 tatctgttaa taccgaacca ttttgaaaaa aactcaacgc tcgtgctttt aagctatcgt
     3301 tatatatccg ttcggtctgt atatcctctt tcacacgtaa cgctttataa ccacaactca
     3361 tgcatgttgg tttacaacgt tctgaaccat ccttattttt agctcgccaa ctatacaaag
     3421 gttcgctaca ttctggacat tttccgcttt gcactaatac tcttcttatt agtttctcca
     3481 tagcatttgc taggctttcc atgtgatgca tctccttttt aaattggcaa gtcgtcatat
     3541 tcactaggat tgctgtactg tagtttttga ctttgctttt tatgattctt cttgtctgct
     3601 ttgatttcga atttgagctt ctcaaatttt tctctcaatt tcttagcact tctaatattt
     3661 ccaaaccaaa attcatttgt aggtagccaa ttgatcacat actcaatcgc ttctatagat
     3721 gctttatctc tttcttccat caacctgatt gtgtctgccc atttttcgat atctactttg
     3781 ttcatttctt ttggaaaatc ttcagttaaa ttactttgca actttttagc aaggcgtaag
     3841 tgttcgtcag aatacttacc tttcttttct tctttatcta tatctttatc ttcttctata
     3901 tctttatctg taccgtcacg tgacgtcacg ctaacgtcat tttccaattt gagacgttcc
     3961 tgtctctttc tttccctgta tttacggttt ctttcagcat tttttagcct tactttatcc
     4021 ataccctcga tattttgatg tttttcccaa ttactgatgg caattagtcc atcactgctt
     4081 agatcaatca tgttgaaatt tgccaatgta gttagcgcta agcgaaccgt atttacgttt
     4141 ttgccaaaca atgtagcaag catttcttcg gtataaggca tgttcctctg gatatatatc
     4201 agaccatcgt cgttagtctt tcctgctaaa actagtaatc gaatccatat aacgatgatg
     4261 gcatccgact caggaacagc ttggattaat cgtatttttt catcgtcaaa catagtagtt
     4321 ttaagtttga tccaacttat ctcagccaaa tttatcctcc tatccttaac tttttaattg
     4381 tttcctggtt taacttgatc cctttgattt gatatttatt tttgaaattg atcacaccta
     4441 ttttgtgttt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac tctgaatgat
     4501 caacttcttt gcgctttcgt cttcccagcg ctttgtcaaa gtgatcgata tcagctcctg
     4561 ttttgccaca gatgcagcag actctttttg taatgcattt gtagaagtaa tactcttgat
     4621 tcgctggtaa aatctcatat ccttctttga aaggaatatg atgttcaaag atgaaatcta
     4681 ggatgatatt cgctaagata ttggcatcac tcacggttgt attcgattcg tctttgaggc
     4741 ttatttcgcg ccctgtgaca ccttcaaaac ggaagtagaa gaattccttc cagaagtccg
     4801 ttggcatgcc tgtatcgatg aaaatatcgc ctatgagcgc atagatgaag tttctttgct
     4861 gtactgtgaa ccgtcgtgga tcaataaagc gaatttcaat gactcgatcg ccatcatatc
     4921 cgtcatacat cgttttcagc cgttcgatat ttacttcttc gttgattgtt gcaccaatgt
     4981 cgttaccttt aaatttcttt agtaccgccg agtatgaatc tattagtggt ttaaacactc
     5041 atatcacttc tcttttgttt cttctctgta ctgatcttca agccaattaa cgcctcgttt
     5101 tagaatgccc aagtctctct tggtccattt actgtcatca gcggttatgg aagctgcatc
     5161 agtcaatgca acaattgctt catcaattga tttttcgtac ttgttagcaa ccagttgtaa
     5221 attcgatttg ccccaatcag gatcggccaa atattgatct agagtggaaa gttcattttc
     5281 catatgttaa aatctcctta gttatgattt tttgagtgac tcattgcttt ggtcggcgga
     5341 gtcacttttt tatttgttgc catgcttttt tcttttcgat atgttgcttg cttaaaataa
     5401 tagaacggct atttgcccac caattatcag caatcacttt accgattttt agcgcttctt
     5461 ctcgtgccat agttgctcct ttcttttgag tcaagcagat tgattaaaac catcaatgct
     5521 gcgaacaagc ttcctccgat aatactttgg tgcgctacaa tcactaataa ccccaaaatg
     5581 aatcctataa aaagtgtgtc tgtcttcttc ataatctaat ctccctattt tttatttcta
     5641 gcattctcaa atcctcaagt tcagaagcaa ttagttcagc ttgtctatct gatagctcat
     5701 cggattttct aagcgctgca cgatcatctt gtaattgttt tctacgctgt ttaatcagac
     5761 ctagaatttg atgttcttgt tgcaatgtgt aggacataaa atcattctcc tttgccttta
     5821 gaactcaaag ttttctttca aaaatctttg gagttccgat cgttcaattc tgatgtctaa
     5881 cttgctccac tgctgtgttt ttaagcctag gcttatccaa tgtgttaatt tgtcatcacc
     5941 aatgcctaaa acttttttta cctctgattt gtttggatat ggaggaagct ctactgattt
     6001 gttcataagg tgtaatcgtt cgtccaatga attaagaaca gcattcgtaa tctgtgtagt
     6061 taattcggaa actactaaat tatctggaat tgttatttgc atgttatttc tccttctcta
     6121 actctgctag tgctgcctca attggcttga tttgtttatc tggttttcta cgcccattca
     6181 taatgtccga catatatggt ctagaaattc cgatagtatc agctaaccag ttctgagact
     6241 ttccgtattt tgctaattga attctgactt tcaaaataaa ttcttgtgac atcgtcattc
     6301 ctcctctaat aaatcgattt ctggaatata tccttctttt ttcagcgact cataaatgaa
     6361 cagacgccct ttttgagtcc attttgtatt catcacaact tttgttccgc catcagattt
     6421 cggaatctca gttgtatgag attttgtgta accctgtctc atgtgttttt tgcataataa
     6481 ccattggttg cctacttttt tctgaatacc tagcttatga agtagtttgt tcatctgttg
     6541 tggagacatc ccataatcag ccgcgatttg actaatagtt actgaatctg tagaagataa
     6601 gatactatcc aaatacgaaa ttttgggttc gtattctgca attttttgtt ctgcgattag
     6661 tcttccagta cgttcttctt tcaacttagt tgctaattgg atgattgtat ctggattaag
     6721 caaagcttct tctacttttt ctggagttag ataacctcca tgttttctaa ttgctggcaa
     6781 cacttcgcta gtcacccaac gtttaaattt cttagcattt ggttgtttgc ttcctaaaat
     6841 taaggagtac aaacctgatt cgttaataat agttaaccct ctaggcgatt caaaagtacc
     6901 gttttggtag ttttgccgat cttctacatc gacgtgtctg ttaatatctc tactaccatt
     6961 ttggtaccct agaactttag ccacatcttt tccaacaaaa aatggttcat catgtattgt
     7021 tactgttctt acttcatgtt gttcaaagtt aaaaatttgc ggtgtgttca tttttgctca
     7081 ttcctttctt tgatataatt ttgaatagaa agcgaggtga aaatagtatg gaagaattta
     7141 atatggatgt cgacgcctta ttcaaacaaa ccgtattcaa gactgttaat aaagaattca
     7201 aaatagactt ccaaaatgac gaatcttttc caacagaaat tgaattattc gaagaacttt
     7261 ctcagagcgt ttctgagtct ttttctcgtc agttgcaaaa gaattttttt gatgctcttg
     7321 tggatgaatt tcatcaacaa cagcactaaa cttttggtct ttaaaattta aagttattaa
     7381 agctcctttt gcggaaggag tttttttatt ttgtttcatg atgtttcctc cttttcttta
     7441 aatatgtaag ctaataaaat tagctaattt ttattgacat ttctatccaa ctgtcttata
     7501 tttatataca gttaaataag cctagaaaaa ccctaaaaat taacattctt agtttgccga
     7561 cctcgaattc gtttatttta ttaggtgtat ttcttgttgc ttgttagctt attaaattag
     7621 cttacgagtt aaatataaga caattggata gatttgtcaa ctacctttct atctttttgt
     7681 ataaattatt atcgtacgct ctaggaaggt tgatttgaca tgcttttcga aagggttaaa
     7741 gagctctgta aaaaaagagg tatttctatt tctgaattag aaaatagaat aggatttgga
     7801 aaaaactcta tttataaatg gaagacacaa agtcctaaaa tagaaacttt gcaaaaagtt
     7861 gctgactact tccatgtatc aacagattat ttgctaggac gcacagatga tcctaacgcg
     7921 ggagttgcac cagaggaaag aaaactaacc gtggaagaag ctttagcatc tgttatgagt
     7981 agcgacggaa aaccgctcac cgataatgat agggaaatac tatcagctat gattgaagca
     8041 tatttagaga aaaaagataa ctaaataagt aggtgagtca tttggacagt caaattgaaa
     8101 tgataattaa tgaactcggc gttaaggtag aagagcgtga aaaccttgat gccgatggcc
     8161 attatgttgc ttgtatgaat accatagtaa taaaagctaa tttatctaag tatagaagac
     8221 aaagaacctt attacatgaa ttaggacacg cttctaaaca tcatgataat tattttttat
     8281 ataacttagc attctctctc cattcaaaaa tggaatatga ggctgatcgc ttcatgattg
     8341 aaaaattatt agatagatat attgcaaagt ctgaattaga accacacaat atcaattaca
     8401 tgaaatttat agaagataac aatttaagcg ttcgcttcga accacttgtg aaagaattat
     8461 taaaagctcg catctattgt tatgcagctc tctaaaattt tttaagcaaa aaagaacata
     8521 cgttcaaaaa tagaaaggtg aacaaaaatg atatatacag aattcaaaga atggttagaa
     8581 aaaagcacaa ccggatacga aacatttatc atcaaagcta ctaattatca aattgaaaaa
     8641 aacaaaaata gaccccaaaa aaaacgctgg gatgataaga aaatagataa agctgtatta
     8701 gaaatgtgga aacaagtcgt gactaacttg tatcaaacaa ttcgtaaaga aaaaggagtt
     8761 ccattaatta acgggaagga aatatggctt gaatttatag aggaacaagg actgatcgaa
     8821 tttttcaatg atagcatggc agaattagaa tttgaatagg ggtaatattg atggcaatga
     8881 taaaacaata taaaaagaaa aatggcgaaa aagcatggta ctttaaaact tatctcggta
     8941 ttgatccgct aactggaaag aaaaaatata ctactaaacg aggatttaga acacaaaaag
     9001 aagcaaaaac agcactttct aggttagaac tagaattaca aaaaacagga atgcccacaa
     9061 gtacaaatac tactttcaaa gaagcagcag aattatggct agaaagctac aaaaaaactg
     9121 taaaagaaag ttcatattca aggactaaaa taatctttaa taaacatata tatcccaaat
     9181 ttggaaatat taagctttct aaaattaata cggcatattg tcaaaaggta gtaaatgatt
     9241 ggagtgaaaa aggaacttca aagcagtacc ctcttttcat aaactatatg aataaagttt
     9301 ttaagtatgc tataaatatt ggtttaacat ctgataatcc aacattaaat ttacttattc
     9361 caaagccaca aattaaaaca gaaaagaaat taaaattata tacaaaagaa cagttggaat
     9421 tatttctaaa tgaagtatct caagaacaga atccatattt taaaaacaga gactatacgc
     9481 tctttagact attagcattc agcggatgta gaatcggcga aatattagca ctcacttggg
     9541 acaatattaa ttttaaaaca aatgaaatgg ccattaaaaa aactgtagct cgttcagata
     9601 aatattatat atctgaaact cctaaaacca aaaaatcaaa tcgaataatt tatttagatg
     9661 aaaaaactat aaagcaacta aaattttgga agctcgaaca aagaaagtac ttatttcaat
     9721 taggatttac taaagctaat tatttgttta ccaatgacga aaataatttc acaattaatc
     9781 agtcagtggc agaaagatac aatatatatc gtgagcgtgc cggcttacct tatatcggtc
     9841 tgcatggttt tagacataca catgcatcaa tgctatatga ggcaggcgca gatcacaaag
     9901 aagtccaaga aagaatgggc cacgcaaata taaaaactac tatggacaca tatacacaca
     9961 ttactaacag caaaaaagaa gaaacaacac aaaaactaac aaattatatt aacttctaag
    10021 aaagtatggt caaaagtatg gtcaaaaaaa atcccataat ttacaaaaaa gctccatccc
    10081 ttgctacaca agagatagag cagtttattt attaagcttc ttgagctact ggataaacag
    10141 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    10201 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    10261 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    10321 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    10381 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    10441 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    10501 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    10561 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    10621 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    10681 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    10741 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    10801 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    10861 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    10921 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    10981 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    11041 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    11101 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    11161 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    11221 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    11281 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    11341 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    11401 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    11461 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    11521 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    11581 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    11641 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    11701 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    11761 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    11821 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    11881 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    11941 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    12001 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    12061 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    12121 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    12181 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    12241 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    12301 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    12361 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    12421 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    12481 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    12541 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    12601 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    12661 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    12721 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    12781 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    12841 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    12901 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    12961 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    13021 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    13081 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    13141 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    13201 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    13261 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    13321 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    13381 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    13441 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    13501 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    13561 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    13621 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    13681 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaatt
//
LOCUS       DACCZY010000076        13599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCZY010000076 DACCZY010000000
VERSION     DACCZY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.077"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            476..718
                     /locus_tag="IVZ67_001880"
     CDS             476..718
                     /locus_tag="IVZ67_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP8165952.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            715..1185
                     /locus_tag="IVZ67_001881"
     CDS             715..1185
                     /locus_tag="IVZ67_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8165953.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1313..2527
                     /locus_tag="IVZ67_001882"
     CDS             1313..2527
                     /locus_tag="IVZ67_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8165954.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(3237..3425)
                     /locus_tag="IVZ67_001883"
     CDS             complement(3237..3425)
                     /locus_tag="IVZ67_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165955.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3516..3725
                     /locus_tag="IVZ67_001884"
     CDS             3516..3725
                     /locus_tag="IVZ67_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP8165956.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3837..5450
                     /locus_tag="IVZ67_001885"
     CDS             3837..5450
                     /locus_tag="IVZ67_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP8165957.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5485..6696
                     /locus_tag="IVZ67_001886"
                     /pseudo
     CDS             5485..6696
                     /locus_tag="IVZ67_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6719..7645
                     /gene="glsA"
                     /locus_tag="IVZ67_001887"
     CDS             6719..7645
                     /gene="glsA"
                     /locus_tag="IVZ67_001887"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8165958.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7853..9313
                     /locus_tag="IVZ67_001888"
     CDS             7853..9313
                     /locus_tag="IVZ67_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165959.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9534..11297
                     /locus_tag="IVZ67_001889"
     CDS             9534..11297
                     /locus_tag="IVZ67_001889"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8165960.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11381..11839
                     /locus_tag="IVZ67_001890"
     CDS             11381..11839
                     /locus_tag="IVZ67_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8165961.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11948..12631
                     /locus_tag="IVZ67_001891"
     CDS             11948..12631
                     /locus_tag="IVZ67_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8165962.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12778..13392
                     /locus_tag="IVZ67_001892"
     CDS             12778..13392
                     /locus_tag="IVZ67_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8165963.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13408..>13599
                     /locus_tag="IVZ67_001893"
     CDS             13408..>13599
                     /locus_tag="IVZ67_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165964.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFN"
BASE COUNT         4721 a         2158 c         2850 g         3870 t
ORIGIN      
        1 tttttccgcc atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc
       61 gttcaaagcc cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa
      121 caagcggaaa acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt
      181 gtacctatat cactatgaaa attgaatagt aaatatgaga aagacagttg atttcaaaaa
      241 ataagaaatt gattgtcgtt ttcttttgca gaaatttaag tgcaggcaat tatggtagtt
      301 gtcgtaattg ctgtcagctc agtgtttatc actgatggtg tgaaagattt acggttggag
      361 atgttcaata agattattgg tgcttaatta tttttattgt agtgctgata tgagaatgtt
      421 ataattgggg tataaaacat acaaataaat ttgctgaaaa atgaggtatc aaaatatgga
      481 aaatgaatta ctggaaaact taggaagact tcacacaact gaacttggcg ttattagaat
      541 taaaaagaac ttatctttga atgtagaaaa tgtaattgaa tggtgcaaag aaaaaattag
      601 cctttctaat gcggaaatca caagaaaagg aaaaaactgg tatataacga ttgataattg
      661 tattataacg gtaaatgcgt acagttatac cgttatcact gctcacaagg taaaatgata
      721 aatttttcta taaaggaatt aaacgcattt gcagttattg ggcaggaagt agaactgact
      781 aattatcaaa aaaagaatat tcagattagt acacagtttt ggagaaagtt taacagtagt
      841 ttgaaaaaag cgtatctttc acaatcagga aattgggtaa aatatgcttt tatggaaaga
      901 agaaatggaa aactttatta tttctgttct attccccaaa gaaccattac cccagagggc
      961 tttctgtata aagaaatacc gtcttataaa tatttggttg tggagcatat tggtgctatg
     1021 gataaaatat atgaaactta tggcaagatt tatgaagaaa ttattcctag tacatcgtat
     1081 attcctataa aagatattat cctacacttt gaaaaatacg attatcgttt tcattggaat
     1141 agtgataatt cagttattga aatttggata cctattcaag attagaagca attttatatc
     1201 catacacaca aagcagttag tcttaggatt gactgctttt tctatacaga tttttaatga
     1261 caggcaatta tcaattatgg tagttgcctt ttttgattgg aggaattgaa gaatgaatga
     1321 taaaaacttt attgaagaac taagacaaaa gcgtgaggaa tacggagtaa cacaaacaag
     1381 gcttgctgtt gcctgtggta tcagccgtga atattacaac cgcattgaaa aaggaaaaca
     1441 gccattgaat gacgaattaa gagaaatcat agaaaaacag attgagcgtt tcaatccgca
     1501 agaaccacta ttcttattga ttgattactt tcgtgtccgc tttcctacta cggacgcatt
     1561 ggcgattatc cgtgatgtat tacaactgaa atctaattac atgctttatg aagattatgg
     1621 aaaatacgga tatgaaagca aatatgtact tggcgacatc aatatcatgt gttccatgca
     1681 ggagcattta ggtgttttat tggaactgaa aggcaaaggc tgtcggcaaa tggaatgtta
     1741 tctgctggca caggaacgct catggtatga cttcatgctg gattgtatga cggctggtgg
     1801 tgtgatgaaa cggcttgatt tggctatcaa tgataaagca ggaatattgg atattccaaa
     1861 gttaaaggaa aaatacaagg ctggcgaatg tatgtcctac ttccgtatgc agaaagatta
     1921 cagcggtaca gaaaaatgcg gtagcgattt accaaagaat acaggagaaa ccttatatct
     1981 cggttcaaca agtagcgaat tatatatgtg tgcttatcag aaaaattatg agcagtatgt
     2041 caagaatggc acagaaattg aagatacgga gattaagaac cgttttgaaa tacgcatgaa
     2101 gaacgaacga gcctattatg cggttgtaga tttactgacc tatcgggacg ctgaacgcac
     2161 cgctttttct atcatcaatc attatgtccg ctttgttgac agagaggacg acaagccaaa
     2221 aagtcaatgg ataacaaatg atgattgggc atggtttgta ggggaaaaca gagagccgat
     2281 acgcttaacc acaaagccag agccttacac tttacaaaaa gcgttgcatt ggctacaaag
     2341 acaagtcgca ccaaccataa aaatggtaca ggcattagac agagaaaatc gtacaacgat
     2401 actgaaagat atgattgagc aggcagaact aaaagacaag cataaacatt tattacaatt
     2461 agagaaatca accatagaag aacgtataga taccgctgtt ccacaagaga atgacggtat
     2521 tttttaatgt aattgaaaaa tttcagattt agtcagcaaa gagctgtttt cattccctat
     2581 gtgaagtgaa agaagaaaaa taaatttttt attaaatctg caacaaaatg gctacttgcg
     2641 tacagatatg gtgcgagaaa aggaaatcta aacttttttt gaaaataggt cattaaatcg
     2701 gggcttgctg tccctatatg aagtgaaaga agaaaaaagt tttgaaaaaa tgacggaaat
     2761 tcgtgattag tggacagtct tctgtgaaaa gaggaaaaac atcatcattt tgtattcaaa
     2821 caggcattag cagttgagtg ttgatgtgaa aagatgaaaa tactttgttc tcaacttaac
     2881 aatttaaggt ttccgttccc tatatggtgc aaagaaaata tttcattcta ttgttggcag
     2941 taacggtatg gtttgggtag ttagggtgtg aaaaggaaaa tattcttttt tcatcatcaa
     3001 cttagcaaaa tgacattttc tatccctata tggtgcatga aaagaagtta aaatttttcc
     3061 atttaaactt agcaaatgga cgtttgctgt acagatagta gtgaaaaagg agaaaatcaa
     3121 ccgttgattg ccaaaatgaa ccgttccatt tgtactaata atggaaaaag ttttggattt
     3181 tggttacgtt tctcctcttt tccaacgcga tatataggaa agacgatttt tttctttcaa
     3241 tcgttctttg acaactgaat aaagctattc aatacgtttg acagacagct agccgttgaa
     3301 acaaatacgc cacgaccttt cccctgcaag agcgagcgaa aacctatttg tgttcttgta
     3361 aaagatttgt cctcttttat cgccatgacc tgtactgtca gataatgata ctcccgtaca
     3421 gccacagctt gagcattaag agcgtcgcac gcaatgggta cggctacata agaactatgc
     3481 agaggtggaa ctcctgtggg ctatgacaaa agccgttgaa ttgcttagaa agattttaca
     3541 gaaagggggt atgtgttatt gataatgctg taaaaacaat tcaaaagaaa aatggcaagt
     3601 gtggcattgg caacagagga aagacaaaga ttgtagtaaa agagcatttt tccgaaaagg
     3661 gcaaaacaat ggaagaactt ctaactgatg tcatgctgga aaaagcaaag cagacaatcg
     3721 cataaaatcg gtgcagttgt aagaaataga ttgaatgaca aaaagtaccc gtgttatact
     3781 ttacttgcaa gcaggtattg taaacacggg ttagttataa aggaggataa ctgacaatga
     3841 aacaacagat ttacaatact gcactttatc ttaggttaag ccgagatgat gaattacagg
     3901 gcgaaagttc cagcattacc acacagagaa gtatgttgcg tttatatgca aaagaacatc
     3961 atttgaatgt cattgatgaa tatattgatg acggctggtc gggaacaaat tttgacagac
     4021 cgagttttca aagaatgatt gaggatatag aggcaggaaa aatcaactgt gttgtaacga
     4081 aagacctttc ccgtcttggc agaaattata ttatgacagg acaatataca gaattgtatt
     4141 tccctagtca taatgtccgc tacatagcga ttgatgacgg tgtagacagc gaaaaaggcg
     4201 aaagtgagat tgcaccattt aagaacatca tcaatgaatg ggtggcaaga gatacaagcc
     4261 gtaaagtcaa atcagcattt aagacaaagt ttgcggaggg ggctcattac ggtgcttatg
     4321 ctccgttagg atataagaaa caccctgaca tcaaaggaaa actgttggtt gatgatgaaa
     4381 caaaatggat tattgaaaaa atcttttccc tagcctatca aggttacgga agtgcaaaaa
     4441 tcacaaagca gttaagagca gaaaaagttc cgacagcgtc atggctaaat tttacaagat
     4501 acggtacttt tgcacatatc tttgagggaa aacccgaaag taagcgttat gagtggacga
     4561 ttgctcatgt caaggcgata ttgaaaagtg aggtttatat cggaaacagt gttcacaata
     4621 tgcagtctac ggtatcgttc aagagcaaaa agaaagtgcg taagcccgaa agtgaatggt
     4681 ttcgagtaga aaacactcac gagccgatta ttgacaagga agtgttctat cgtgtgcagg
     4741 agcagataaa atcaagacgc agacagacaa aggaaaaggc aacgccgatt tttgcagggc
     4801 ttgtcaagtg tgcggattgt ggttggtcta tgagatttgg aacgaataag acaaataaaa
     4861 cgccttacag ttattatgct tgcagttact acgggcagtt tggcaaaggt aattgttcta
     4921 tgcactacat tcgttatgat gtgctgtatc aagccgtatt ggaacgcttg cagtattggg
     4981 ctaaggcagt acagcaggac gaagaaaagg tattgaacaa gatacagaaa gctggcaatg
     5041 cagagagaat acgggaaaag aagaaaaagg caagtacatt gaagaaagcc gagaacagac
     5101 aaaatgagat tgaccgttta tttgcgaaaa tgtatgaaga tagagcctgt gagaagataa
     5161 cagagcgaaa ttttgtgatg ttgtccagca agtaccaaaa agaacagata gaactggaac
     5221 agcagataac aagcctaaga gaagaactaa gtaaaatgga acaggatatg ataggtgctg
     5281 aaaagtggat tgagttaatc aaggaatatt ccgtaccaaa ggaactgaca gcaccattat
     5341 taaatgccat gatagaaaaa atcctcatac atgaagcaac aacgaatgag gataacgaaa
     5401 gaatacagga aattgagata tattaccgat ttattggaag agttgagtaa tcaacaagag
     5461 taatattttt aactaaggga aaccggcggt gtctatactt gggtcagtcg cacattggga
     5521 aaacgattcg gttttgccgc aatctttttc caatggtttg agataacagt tggctacttg
     5581 accatgctgt atttcttaac tggtgcatta tcctatgcaa cgggtattgg tgctatacaa
     5641 aacaataaat atctgaaatt ggcaatccta ttgattattt tctgggctat tttactctca
     5701 cagttaaaag gaaccaaata cacttcattg attgctcgga tcggttttgt agctggtatc
     5761 ttgttgccag cattagtctt gtttggctta gggatccatt atgtcgcttc aggcgctcca
     5821 ctacaaacga tactgtctat gaaaacattg attcctgatt tttcgaaatt gcctacgctg
     5881 gttgtttttg tttcctttat tttagcatat atgggcgtag aaacatctgc cagtcatgca
     5941 aatgaaatgg aaaaccctaa aaaggattat ccattagcaa tgtttgtatt ggttattctg
     6001 gcaatcattt tagatacttt tggcggattg accgtcgcta ctacagtacc tcaacatggt
     6061 ttgtctctta atacaggggt gattcaatcg ttagaattcc tattaagaca cattgatcca
     6121 aatcttgcat ggattgcgaa aattcttgcg attcttgttt gtttaggtgt catcggagaa
     6181 attgcttctt gggtaacctc tccttcaaaa gcacttcacg tagcagcaga agaaggatta
     6241 cttcctgaat attttgcaaa agaaaacaca cacggtgttc ctgctcattt gatgatcgca
     6301 aatgggatcg ttgcaacaat ctgggcagcc gtctttacat taagcggcgg ggggaataat
     6361 ctttcatttc ttgcagcgat gtcattgact gttgtaatct acctgatgtt gtactttatg
     6421 tttttcgcgg gctatttgaa gctggttttt aaagatgaca gtcttgaaag agcctatcag
     6481 gttccaggcg gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt gacttcactt
     6541 tttgcttttt gtatctcatt tgttccacct gctagtatcg cagctggaca gcatgggatt
     6601 tatgaaacga ttctgattgt ttcttatctt atcgtcttag ttattccatt tacgatctac
     6661 gcatgccgtc ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg ttattcaaat
     6721 ggaagtaaaa gaattagaac aattgattgg aaaaaatttc cactattatc aaacaggaca
     6781 agtggcggat tacattccag cattagctga agtggatccc aaacaattag gtatggcgat
     6841 ttatgatttg gatacaaaac aactgattga gggaggagac gccgatgtgc gctttgccat
     6901 cgaaagcatg tcaaaagttc ccgtattgtt attagcaatc aaagacaacg gaatagaaaa
     6961 agtctttcaa acaatcaaca cggaaccaac cggttttgcc ttcaattcta tcatgaatat
     7021 ggaaatcaat catcgcaaac atccaatgaa tccttttgtt aatgcaggag cgattgcaac
     7081 aacctcgttg attgccggta aagatgcaga tgaaaaattt gagcgaatct tgtcatttat
     7141 gaaagagatc tgtgatgatc ctaatatcac attgaatgaa gaaatctatc attctgaatc
     7201 aagaacaggt gatatcaatc gctctcttgc ttactatatg aaaggtaatc aaatgatcga
     7261 aggagacgta ccggagatac tcgatgctta tttcagacaa tgctccctca atgtcaccgc
     7321 tacaggcata gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc cttggaatgg
     7381 caaacaaatc attactgaag aaagtgctac aatcgtcaaa tcaattatga caactgctgg
     7441 tttgtatgat gaatcaggag agttttccgt ccatgttggt gtacctgcaa aaagcggtgt
     7501 aggcggcggg ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct tcagcccggc
     7561 actagatcca tttggtaaca gcgcagctgg tatccaattg ttgaaagatg tagtcaaaga
     7621 actagacgct gacatctttg aataaccatt cttgaaagag gctggggtaa aagtccaaac
     7681 ggctcaaagc tacctacaat gaggctgaaa ttgtgagctt gtttgacttt tgtcccagtt
     7741 tcttttttta ttggtttttg aaagggcttt tctaaataaa aaggaaacaa aaatacgatt
     7801 tacagatatg attaaataaa atcataagaa aaactggagg cgtgtatcag gaatgctgga
     7861 aaattacatc gagcgaaata tatttagaaa agtgtatctg tgtgaacaat tatttgaatt
     7921 ccaagaaata gacatcgaac aaacagctat ttctctaagg gtgactacac caacgatcct
     7981 tcacgatcta gaaagtctag ccgagtgtct agaatattgt ataaaagaac aagtaagaga
     8041 gaagcataag tataaactcg tattcaaaca tggtattgct ctttctgagc tcacacagtt
     8101 tctgtacggt caatcttatt ttttaaagtt tttaagttat tattttaatg gtatattcac
     8161 gagtacagaa ctggctgatc ttgaatttat ttctttgagt aaagtatata caatcaaaaa
     8221 aatcgtattg gattttttta aagaaaatga ttatttgaaa aataaagaaa tcattatccc
     8281 agaatttgac tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt gggaaggcta
     8341 tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt atgtagagac
     8401 tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac gagaaataga
     8461 gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata agaaaagcgc
     8521 agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa aaggaattct
     8581 agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta gaaattatac
     8641 caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct
     8701 caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga
     8761 tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga
     8821 tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta
     8881 cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg
     8941 gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc
     9001 ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta
     9061 tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt
     9121 tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg
     9181 cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc
     9241 attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa
     9301 acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt
     9361 agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc
     9421 tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag
     9481 tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta
     9541 cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg
     9601 aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg
     9661 taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg
     9721 tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata
     9781 agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat
     9841 attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta
     9901 tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg
     9961 tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg
    10021 gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg
    10081 agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca
    10141 gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc
    10201 tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc
    10261 acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc
    10321 attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa
    10381 catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta
    10441 ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac
    10501 atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg
    10561 ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg
    10621 catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg
    10681 gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact
    10741 tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta
    10801 tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg
    10861 gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa
    10921 ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa
    10981 cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca
    11041 cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat
    11101 tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt
    11161 cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag
    11221 ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg
    11281 tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat actagtatct
    11341 ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga
    11401 atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt
    11461 ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat
    11521 aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa
    11581 agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa
    11641 tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg
    11701 agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc
    11761 ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt
    11821 tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgtctcc
    11881 tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca ttactggagg
    11941 agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt
    12001 ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga
    12061 aggcggagca gttggtattc gagcaaatac gacagaagat atcaatgaaa tcaagcgaat
    12121 cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat
    12181 tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact
    12241 ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat
    12301 tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc
    12361 gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg
    12421 aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc
    12481 agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg
    12541 tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata
    12601 tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa
    12661 aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt
    12721 ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg
    12781 tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca
    12841 gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc
    12901 gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc
    12961 cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct
    13021 gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg
    13081 cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt
    13141 tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc
    13201 gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat
    13261 catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat
    13321 gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca
    13381 cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat
    13441 tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta
    13501 gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa
    13561 atagaaatgc cagatcaaac gattagcgga ttgtttaat
//
LOCUS       DACCZY010000077        13506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCZY010000077 DACCZY010000000
VERSION     DACCZY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.078"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            115..390
                     /gene="rpsP"
                     /locus_tag="IVZ67_001894"
     CDS             115..390
                     /gene="rpsP"
                     /locus_tag="IVZ67_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8165965.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            402..653
                     /locus_tag="IVZ67_001895"
     CDS             402..653
                     /locus_tag="IVZ67_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8165966.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            778..1569
                     /locus_tag="IVZ67_001896"
     CDS             778..1569
                     /locus_tag="IVZ67_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165967.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1739..2260
                     /gene="rimM"
                     /locus_tag="IVZ67_001897"
     CDS             1739..2260
                     /gene="rimM"
                     /locus_tag="IVZ67_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8165968.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2250..3011
                     /gene="trmD"
                     /locus_tag="IVZ67_001898"
     CDS             2250..3011
                     /gene="trmD"
                     /locus_tag="IVZ67_001898"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8165969.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3147..3494
                     /gene="rplS"
                     /locus_tag="IVZ67_001899"
     CDS             3147..3494
                     /gene="rplS"
                     /locus_tag="IVZ67_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8165970.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3867..4529
                     /locus_tag="IVZ67_001900"
     CDS             3867..4529
                     /locus_tag="IVZ67_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165971.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            4608..7835
                     /gene="fss3"
                     /locus_tag="IVZ67_001901"
     CDS             4608..7835
                     /gene="fss3"
                     /locus_tag="IVZ67_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8165972.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7949..8263
                     /locus_tag="IVZ67_001902"
     CDS             7949..8263
                     /locus_tag="IVZ67_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8165973.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8276..8650
                     /locus_tag="IVZ67_001903"
     CDS             8276..8650
                     /locus_tag="IVZ67_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8165974.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            8660..9004
                     /locus_tag="IVZ67_001904"
     CDS             8660..9004
                     /locus_tag="IVZ67_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8165975.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            9075..10427
                     /locus_tag="IVZ67_001905"
     CDS             9075..10427
                     /locus_tag="IVZ67_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8165976.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            10487..11629
                     /locus_tag="IVZ67_001906"
     CDS             10487..11629
                     /locus_tag="IVZ67_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8165977.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            11654..11908
                     /locus_tag="IVZ67_001907"
     CDS             11654..11908
                     /locus_tag="IVZ67_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165978.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            12164..13348
                     /locus_tag="IVZ67_001908"
     CDS             12164..13348
                     /locus_tag="IVZ67_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8165979.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            13363..13482
                     /locus_tag="IVZ67_001909"
     CDS             13363..13482
                     /locus_tag="IVZ67_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8165980.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         4555 a         2338 c         2985 g         3628 t
ORIGIN      
        1 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
       61 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
      121 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
      181 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
      241 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
      301 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
      361 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
      421 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
      481 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
      541 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
      601 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
      661 tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
      721 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
      781 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
      841 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
      901 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
      961 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
     1021 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
     1081 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
     1141 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
     1201 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
     1261 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
     1321 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
     1381 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
     1441 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
     1501 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
     1561 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
     1621 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
     1681 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
     1741 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
     1801 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
     1861 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
     1921 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
     1981 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
     2041 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
     2101 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
     2161 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
     2221 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
     2281 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
     2341 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
     2401 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
     2461 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
     2521 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
     2581 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
     2641 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
     2701 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
     2761 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
     2821 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
     2881 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
     2941 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
     3001 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
     3061 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
     3121 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
     3181 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
     3241 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
     3301 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
     3361 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
     3421 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
     3481 aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga aaagtccttg
     3541 tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt ggctgttttg
     3601 gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca ttgactatca
     3661 gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata atacgaaaaa
     3721 taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa gggtcagcta
     3781 ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat aggcttctat
     3841 ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc atttggaaaa
     3901 atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa cacggaatta
     3961 catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca ccaagtatgt
     4021 ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata ggacgagaaa
     4081 atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa atagtagaag
     4141 cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa tcagtaggga
     4201 agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt ccacttttta
     4261 aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa ctaaaaaatg
     4321 aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga aaactagttc
     4381 aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa aattgggagg
     4441 aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag cagagaaaca
     4501 acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt caaaattaaa
     4561 atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg aaaaagccaa
     4621 gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca attggtggat
     4681 cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg gtaactgtgg
     4741 aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt cgtcccatgt
     4801 cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat attttctgta
     4861 ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa aatccattgc
     4921 ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct ggtacggatg
     4981 tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc tacacgcttc
     5041 actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag tcaaaaatca
     5101 atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca gtgaaaaccg
     5161 tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg gagtttgatg
     5221 aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta
     5281 ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca gctaatacag
     5341 gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg gcaattgata
     5401 agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg aagattaaaa
     5461 aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta gactttggaa
     5521 aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca ttggatgaga
     5581 ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct tatacgattg
     5641 acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg acttcaaaaa
     5701 atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca ggaactgatc
     5761 tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa gacagtccaa
     5821 ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag
     5881 agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa gctagtaatg
     5941 gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat caaaccgtgg
     6001 ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa accactttga
     6061 caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt aaaggagctg
     6121 aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag gcttttaaac
     6181 cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact ttagatgaaa
     6241 agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
     6301 cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa aaggtggatg
     6361 ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa gtcattcgct
     6421 ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa actggcttca
     6481 atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca ggtgccgaag
     6541 atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc aagtttgaaa
     6601 atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga tttcaaaaaa
     6661 ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac actaagagtg
     6721 aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc
     6781 cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg atgctctatg
     6841 atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac ggaggcaaag
     6901 aattgacaga ccttgattct accgagctag ttgataaatt gagctataac ttgcatgaaa
     6961 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc
     7021 aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca acgttggcga
     7081 ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa caagttttgg
     7141 ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg
     7201 atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc aattgtacgg
     7261 tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg caaaccttta
     7321 ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat gtactggatg
     7381 gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat acgaccaaag
     7441 aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc actaaaaccg
     7501 tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt accttcaaag
     7561 aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat ctaaaagaaa
     7621 aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg
     7681 aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag acattcccac
     7741 aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg atctttgcga
     7801 ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa caactaaata
     7861 ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca
     7921 aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc cagatatggc
     7981 cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg
     8041 gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc aacgagccga
     8101 tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc aagatcaacc
     8161 actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt
     8221 taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa agaagatgag
     8281 attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac gattttccgc
     8341 attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg aggtaaaaga
     8401 acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta gtatcccagc
     8461 aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca atccaatcat
     8521 ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt tcattaaggc
     8581 cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc aaaatgaagg
     8641 aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt gtctcgatca
     8701 atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct atgatgaaaa
     8761 aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg aagaagattt
     8821 tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg aagtaaatga
     8881 aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc
     8941 cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag cagtaaatga
     9001 ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt cgattggagg
     9061 tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag cacttggttt
     9121 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
     9181 ttcaagagct tagacaattt gatatttcta agctatcact ttctactata gaaatcgttc
     9241 gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga agcacgatgt
     9301 tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga caaaagctag
     9361 cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt gaaggatttt
     9421 tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc aaaatctatt
     9481 accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga aaatatcaag
     9541 atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa ctagtagaaa
     9601 aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt gccaatcgaa
     9661 ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa aatcaagtat
     9721 gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt gggaagactt
     9781 actttttact cactatcatc gaggctttat taaaatctga tgccgagctt tttgttcttg
     9841 atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc tattcccaaa
     9901 aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact cgtagcaagg
     9961 caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt ggacttccac
    10021 caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg acgaaggaaa
    10081 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct
    10141 ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg attcgcgatc
    10201 aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc atgatgtttg
    10261 gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat gtagatacgg
    10321 gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat gatttcttaa
    10381 gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat aatagtatgt
    10441 agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga catttttaga
    10501 tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc accagtgtat
    10561 cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc atgatacaag
    10621 taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc aatctctcgg
    10681 accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg cgggaaagcg
    10741 gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccgctat
    10801 tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc acgaaggagg
    10861 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg tggaatggca
    10921 agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc ttatcggaca
    10981 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc caacatctga
    11041 tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg ggggacaagt
    11101 ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca aaatcgcatt
    11161 gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg aacaaggcaa
    11221 tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag gcggaacgaa
    11281 gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga acagtgtgaa
    11341 cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa gtgtgcatac
    11401 cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc gaaagttaac
    11461 acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc gagcttctca
    11521 agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag ttccggtcat
    11581 ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat gagttgattg
    11641 gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag aagaactgta
    11701 ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc cgtatgtaat
    11761 tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat
    11821 tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt
    11881 ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg
    11941 cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag
    12001 ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag
    12061 caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta
    12121 actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt
    12181 agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc
    12241 tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc
    12301 atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc
    12361 tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cgaggaaaat
    12421 cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt acgcctttta
    12481 ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac atgaagaaga
    12541 taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact
    12601 cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat
    12661 gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac
    12721 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc
    12781 cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag
    12841 tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt
    12901 agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga
    12961 tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt
    13021 ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa
    13081 ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt
    13141 aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt
    13201 cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca
    13261 ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag
    13321 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat
    13381 attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca
    13441 agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca
    13501 aaattt
//
LOCUS       DACCZY010000078        13370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCZY010000078 DACCZY010000000
VERSION     DACCZY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.079"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            6..851
                     /locus_tag="IVZ67_001910"
     CDS             6..851
                     /locus_tag="IVZ67_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8165981.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1042..2229
                     /locus_tag="IVZ67_001911"
     CDS             1042..2229
                     /locus_tag="IVZ67_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8165982.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2235..2591
                     /locus_tag="IVZ67_001912"
     CDS             2235..2591
                     /locus_tag="IVZ67_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8165983.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2593..2931
                     /locus_tag="IVZ67_001913"
     CDS             2593..2931
                     /locus_tag="IVZ67_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8165984.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3326..4360
                     /locus_tag="IVZ67_001914"
     CDS             3326..4360
                     /locus_tag="IVZ67_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8165985.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4353..5039
                     /locus_tag="IVZ67_001915"
     CDS             4353..5039
                     /locus_tag="IVZ67_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8165986.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5063..5911
                     /locus_tag="IVZ67_001916"
     CDS             5063..5911
                     /locus_tag="IVZ67_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8165987.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6108..6881
                     /gene="sufC"
                     /locus_tag="IVZ67_001917"
     CDS             6108..6881
                     /gene="sufC"
                     /locus_tag="IVZ67_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8165988.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            6894..8180
                     /gene="sufD"
                     /locus_tag="IVZ67_001918"
     CDS             6894..8180
                     /gene="sufD"
                     /locus_tag="IVZ67_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8165989.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8177..9412
                     /locus_tag="IVZ67_001919"
     CDS             8177..9412
                     /locus_tag="IVZ67_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8165990.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9399..9869
                     /locus_tag="IVZ67_001920"
     CDS             9399..9869
                     /locus_tag="IVZ67_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8165991.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            9874..11265
                     /gene="sufB"
                     /locus_tag="IVZ67_001921"
     CDS             9874..11265
                     /gene="sufB"
                     /locus_tag="IVZ67_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8165992.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11363..12505)
                     /locus_tag="IVZ67_001922"
     CDS             complement(11363..12505)
                     /locus_tag="IVZ67_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8165993.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(12556..13353)
                     /locus_tag="IVZ67_001923"
     CDS             complement(12556..13353)
                     /locus_tag="IVZ67_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8165994.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         4256 a         2478 c         2869 g         3767 t
ORIGIN      
        1 gtgccatgtt taagttatta acgacttttc gagcagtata tgaaacaaga aatttttcca
       61 gagcagcaga agtactgttt ttatctcaac cagcagtctc gaatcaaatc aaacagttgg
      121 agcgggaatt gaatatcgat ctttttcaac gtaacggaag aaaagagatg cttccaacta
      181 agcaagccga cttattatat gaacggacac tggttttgtt agaagaatgg gaagaaactc
      241 agcaaaaatt gctcaatgaa caaaatgaag aagaagtatg tcggatcgtt gcttcccaca
      301 cctttgctgt ctatcttttg cctcagttga tgaaacatct gatcaaaagg tttcctaaag
      361 tgcattttga gatcgaaatc acgaattctc atgaatcatt agaacaagta gcgaaacatg
      421 aatccgattt tggttttatc gaaaagccat tagagacttc cggcgtacaa agatactctt
      481 tgatggaaga ccagctagtc atcgctgggg acccaaaaag caaattgtgg cttgtaagag
      541 aagcaaactc tggtgttttt cattacacgc agcgttattt tgaagagatg aatatccaag
      601 ataagaagat gatcgtgaaa agtaacgatg taattgttgc gttgctgaag gaaggaatcg
      661 gtcgctcgat tttgtctaag cgctctgttc cagaaaatgt gccaatgact ccgttgaatt
      721 tccatcgata tttttatttc atccaacgaa ccaatctggt ttctgatcag ctgctagaga
      781 ttgccgaaga gatccttcga tacaatgaaa gcacagaaac ttctcacagc gaggagaatc
      841 tcagctggta gataattgca tgtatcaaaa aaagttctat gatacactaa agataagcag
      901 taggcttttg tactgcattt gctcaagcaa ctgcacaaac ggaaagttaa tttttcccaa
      961 atgtctatgg aaagactttc cattatctaa ctggaccgct ataataatca gcagtatggt
     1021 ttttaagaaa agggatgaaa catggaaaaa aaattaaaag cttcgaacga taatcgtatt
     1081 gattatggcg tgattttacc agtctttctg ttatgtttga ttggtctgct ctcattatat
     1141 gtagcactta gccatgatcc gaacaatcca gcagtcttga aaggcgtagg gatgcaagtt
     1201 gtctggtatt tagttggagc ggcagcaatc gttgtcatca tgcatttgaa ttccaaatgg
     1261 atctggaaac tgactcctta cctttatggt ttggggttag tggtaatggg actcttgctc
     1321 aaattctatg atcagaattt agctgcttca acagggtcta aaaactggtt tcgattcggt
     1381 gcgtttactt tccagccagc agagttgatg aagattgcgt acatcttgat gatggcactg
     1441 atcgtaacta aacataatac gcaagtaaaa gaacggacga tgaaatcgga cttttggctg
     1501 attgggaagc tcttgatcgt gacaataccg gttttagcat tgatcatggc ccaagatgac
     1561 tttggaacga tgcttgtctt tcttgcgatc tttggcggga ttttcctgat gtctggaatc
     1621 tcttggcgca tcatcgcacc tgtagtaatc ttggcagtag tcgttggagc aggaacgatc
     1681 ttcttggtta cgactgaagg cggaagagac ttgctttata aagttggatt caaatcttat
     1741 cagtttgcac gtattgattc atggctggac cctttccatg atacgtcagg aatgagttat
     1801 cagccggccc aaggactttt ggcaattggt accggtggct tgtttggtaa aggattcaac
     1861 gtaagcaata tctatgtgcc tgttcgtgag tccgatatga tttttaccgt tatcggtgaa
     1921 aacttcggct ttatcggtgg tgcattcgtc attttcttgt attttatttt aatctatcgt
     1981 atgattcggg tgtgtttcga tacgaataat gaattttacg cctatatcgc aagcggtttg
     2041 atcatgatgc tgctgttcca tgtctttgag aatattggag caaatatcgg attacttcct
     2101 ttaacaggga tcccattgcc tttcattagt cagggaggat cctcgatttt agggaacatg
     2161 atcggtatcg ggttgatatt atcgatgaga taccaaaatg aagcacctgc aagatcttca
     2221 aggaggtaag aaaattgtac accttttact ggtacccgaa atgttcaaca tgcaaaaaag
     2281 ccaaagcgtg gttagaccag catggcgtta gttatcaaac cgttgatatg atcaaagaag
     2341 taccgaaggc ctcaacgatt gagacttggc tagaaacgag tcaagtacca atccgtcgcc
     2401 tgttcaacac aagcgggatg aaatatcggg aacttggact gaaagatcaa gtaggcgagt
     2461 ttacggcttc aaaagcgagc caagtcttat cgacagatgg tatgctgatc aagcgaccac
     2521 tgatcttacg agaggaccag cttgttgcaa ttggattcaa tgaaaaaatt tatgaaggag
     2581 aactgatata aaatggcagg caagcatttg aagaaaaagg acaacctttg gatcttattt
     2641 aatggaaaag aatactgtgt tggtttgaca aatgaagcgc aagaagaact tggagacatc
     2701 acgtttgcca atctgccaaa agcaggacaa tcgctaaaaa aagatgaacc tctgatcgaa
     2761 gttgaagcgg aaaaagcggt caatgaattt gccagcccgc taacgggtac agtttcttct
     2821 gttaatgata aaatcggtga agatatcaat atcttgaatg attcagatga aatgaatgcg
     2881 tggatcttaa gtttcaaaga tgttgatcca aaagaatttg acgaattata aaatcagaaa
     2941 atcacttgaa aaaagttgac actgattctt ccagttgcta taatcataag caatcctata
     3001 tgaaacgaga aagctttgaa aagaagagta catgtcacgg acgtttgaag agagccctgt
     3061 ttgctgaaaa tgggtaacga aaatgagatg gaagatggtc ttagagcaga gtaagcgagc
     3121 gtaagcaaac ttactacggg agtgcccgtt acagcgcgac agtatacgga aagaagctaa
     3181 gtccaagtac tggaagaggc tgttattgcg aagtaacggt aaatcaaggt ggtaacacgg
     3241 aagttcaagc tttcgtcctt ttgcttaagg caagagggcg aaggcttttt tgtttgaaaa
     3301 attataagag aaagatgtga atggcatgtc attaatccaa ctaacgaatg tcaaaaagac
     3361 attcaatggg aaaaacggaa acatccaagc cgtcaatgac gtttcactaa acgttgaaaa
     3421 aggggatatt tatggaatcg tcggctattc tggcgcagga aaaagtacat tagtccgtct
     3481 cctgaatgga cttgagcttc ccacgagcgg cgaagtgatc gtaaacgatc aagatattac
     3541 aaaattaaaa aacaaagaat tacgtacatt ccgaaagaaa atcggtatga tcttccagca
     3601 tttcaacctg ttatggtcaa gaactgtact tgaaaatatc cagctgccgc tggaattagc
     3661 tggcgtacca aagagcaaac gtaaagaacg ggcagaagaa ctgttgcgtt tagtcgggtt
     3721 agaaggacgc ggacaagcat atccttcaca actttccggc ggacagaaac aacgcgtcgg
     3781 tatcgcacga gcattggcaa atgatcctga gatcctgctt tgtgatgagg ctactagcgc
     3841 attggacccg caaacaacag aagaagtatt agacttattg ttagcgatca ataagaaatt
     3901 gaatttgacc atcgtcttga tcacacatga aatgaatgtg atccgcaaaa tctgcaacaa
     3961 agtcgctgtg atggagttgg ggaaagtcgt agaagaagga gacgtattga ctgtcttcag
     4021 acaaccgaaa aaagaagtaa ccaaacgctt tgtacaacaa gacatcgaac cagaagctga
     4081 ttcgacagaa ctattagctg aactcatcaa agaaaatcca agcggccgtt tggcaacttt
     4141 gacctttagc gaaagaaatg ccaacgagcc agtcatttcc caagcgattc gacgctataa
     4201 catcgatgtc aacgttgtgt atggaaagat caaacaagtg aaagaaggtt cctttggttc
     4261 attgactgtc ttgatgactg gtgaaaccaa agaattagat caagcagaag tgttcatcaa
     4321 agaacaagga gtaggaattg aggtgatcca tcgtggataa aagcttttca gaaacatatc
     4381 tagattttaa acaaatcaac ccggaaacga tgaatcaggc tatccttgat acactatata
     4441 tgacattaat ctcaatggtc ttagtgacga tcatcggatt agttttggga ttgatccttt
     4501 actctattgg acgcaaaaac aaaccgtatg ctcgaatcat ttatggaatc gtatcgatca
     4561 tcagtaatat tttccggtca acgccattta tgatcctgat ggttttgatc attccattta
     4621 ctaaagcaat cgtaggcact atgctaggtg cgcaagcagc cattccagct cttgttttat
     4681 ccgcagcacc attttatgcc cgtctagtag agattgcttt tcgtgaagtc agccctggtg
     4741 tactggaagc agcagatgca atgggagcaa gttactggga aatcatctgg aaggtcttga
     4801 tacctgaaag tattcccgca ttgatttctg gattaacagt tacaacgatc tcgatgatcg
     4861 gatttactgc gatggccggc gcgatcggcg caggtggact tggaggtctt gcttggcagg
     4921 aaggctacca gcgtggtaat ctaacagtga cattcgttgc gactttaatt attttactaa
     4981 tcgtatttat cgttcaagga atcggtgatt tcttaactaa gaaaacagac aaaagataat
     5041 aggatatagg gggatgtagg atatgaaaaa gaaaattttt ggatttgcag cagcgctttt
     5101 gctgactgta ggattagcag catgcggtaa caacagcgac agcaaagatt cagcaaataa
     5161 agcagacgac acgacattga aagtcggcgc ttcaccgaca ccgcacgcag agattttgga
     5221 acatgtgaaa ccattattga aagatgaagg aatcgatctt gaaatcgtca aattcgatga
     5281 ctatgtatta ccgaaccaat ctttggaaga aggcgacatt gatgccaatt acttccagca
     5341 tattccttat ttgaacaaag aaatcaaaga aaaaggatat gactttgtca atgccggtgc
     5401 tgtccacatc gaaccaatgg gcttgtactc taaaaaagtc aaagatgttt ccgaattaaa
     5461 agatggtgca acaatcatta cttcaaattc tgaatcagac tggggacgca tcatcacaat
     5521 tctacaagat gctggcttag tcaaagtcaa agatggagta gatctggaaa cagcaacatt
     5581 cgatgatatt gaagaaaacc ctaagaactt gaagttcaac catacgatcg acccatcact
     5641 tttagcttct acgtaccaaa atgatgaagg tgacctagtt gcaatcaacg caaacttcgc
     5701 ttacggcgca gggttgaatc cattagaaga tgccgtattg cttgaaaagg acaactctcc
     5761 ttacgtgaat attattgcga ctcgtaaagg tgatgaagac agtgacaaaa tcaaaaaact
     5821 gattgaagtg cttcaccgca aagaagtcca agaatggatc acagacaaat ggggcggctc
     5881 tgttaaacca gtaaaggctg atgcaaaata aggtatggaa caaaaaagat gagaaataag
     5941 tctgtaatgg cttatttctc atctttttta tacctattct aatttagaaa tcaaaattgt
     6001 ttaataataa ataaaagata tttgatttta gtaaggaagg tcattgcgct tcattctcaa
     6061 ttagtctaaa atgagtgaga acgaaagaga atttgggggt ataatgcatg tctgtcttag
     6121 aaataaaaaa tctacatgtg tcgatcgagg aaaaagaaat ccttaaaggc gtaaacctaa
     6181 caatgaaaac cggagaaatc catgccatca tgggacctaa cggcacagga aaatctacct
     6241 tatctgccgc tatcatgggc aatccaaact atgaagtaac agaaggcgaa attttgtttg
     6301 acgggaaaaa tgttttagac ttggaagtag atgaacgtgc gcgacttggc ttatttttag
     6361 ctatgcaata tccaagtgag ataccaggta tcacgaatgc ggaattcatg cgtgcagcga
     6421 tcaatgcgaa acgtgatgaa aacgacaaaa tttctgttat ggcatttttg aaaaagttag
     6481 atgaaaaaat ggctttacta aatatgccag aagaaatggc tgaacgttat ctgaacgaag
     6541 gtttttctgg cggagaaaag aaacggaatg agatcctgca attgttgatg ttagaaccta
     6601 catttgcaat tttagacgaa atcgattcag gtctggatat cgatgctttg aaagtcgttg
     6661 ctaaaggagt aaatgaaatg cgcggagaca atttcggtgc attgatcatc acccactatc
     6721 aacgattatt gaactatatc acacctgatg tcgttcacat catgatggaa ggccgagtag
     6781 tcatgacagg gaatgctgac ctagctaaac gtctggaagc agaaggctac gctggtatca
     6841 gtaaagaatt aggaatcgac tataaagaag aagaagcgta agggggagca agaatgaaag
     6901 aaaaaaactg gctcgattat cttgacgcgg taaatgattt ttctttaagt aaaggcgaac
     6961 ctgattggat gagaaccttc cgtcaagacg cactagcgaa agcagatgaa ctgccattgc
     7021 cgcatatcga tcgggtgaaa ttccatcgct ggtcgttatt tgatgtgaaa gaaacacaaa
     7081 ccatcagtga aacaggcacg attccggcat ttgatgcaat gaaagacaat cctgtgcttg
     7141 tccaacaagg ttcatggacg atttttgaac aactgcctgt ggaattagca gaaaaaggag
     7201 tcatttttac cgacttgttc actgcaatga tcgaatatcc agaactagtc caagaatatt
     7261 atatgaaaaa agcagtaaac atgaacgaag accaattgac tgcactccat gtagctttta
     7321 tgaacagcgg tattttcctg tatgtgccta aaaatgtcgt gatcgatgaa ccactcgagt
     7381 ccttattcat tcaagatggc gcatccgatg agcacttctt caagcatgtg ttgatcgtgg
     7441 cagatgaaca tagtgaattt tcttatctgg aacgtttcca gacaaccaaa gaacaagtag
     7501 caaaatcatc aggtaatatc atcgtggaag tcatcgctaa agctggttca aaaatcaaat
     7561 attcagccgt agatcaatta ggagaaaata ttacttctta tatgaatcgc cgtgggcaca
     7621 ttttacgaga tgcttctgtc gactgggcaa ttggtgtcat gaatgacggt catgtgatcg
     7681 ctgactttga ctcagattta gcaggagaag gtgcacacgc agaagtcaaa atcgtggcaa
     7741 tcagctcggg cagacaaatc caaggaatcg atacacgagt aaccaataaa gcccctcata
     7801 cgatcgggca tattttgcaa cacggagtga tccgagaaaa aggaacattg acctttaatg
     7861 gtatcggcca catcttgaaa ggggcaaaag gtgcagatgc ccaacaagaa agtcgtgtct
     7921 tgatgctttc tgataaagca cgtggtgatg cgaatcctat tcttttgatc gatgaaaacg
     7981 aagtaactgc tggacatgct gccagtgtag gacgagttga tccagaagaa atgtactatc
     8041 tgatgagtag aggattgcat aaagaagaag cagagcgctt agtgattcga ggattcctcg
     8101 gttctgtctt aacagcaatc cctgtagaac aagttcggaa agaattagtc gaagtaatcg
     8161 aagggaagtt gaatggatga tcgatgctaa aaagctacgc aatgattttc ctatcttatt
     8221 tcaagacgtc aatgatgaac ctttagttta tctggataat gcagcgacga cccaaaagcc
     8281 aaaacaagta ctccaggcat tagcgtacta ttacgaacac gataacgcca atgttcatcg
     8341 cggtgtccat acgttagcag aacgcgcgac ccatgattat gaacaagcaa gagaaaaagt
     8401 acgggcgttc atccatgcaa aagaaacagc agaagtgctg tttactcgtg gtacgacgac
     8461 aagcttgaat tgggtagcca gaaccttcgg cgaccgctat atcaaagcag gagacgagat
     8521 agtgctctct tacatggaac atcattctaa tgttatcccg tggcagcagt tggcaaaacg
     8581 aacaggtgct gtcttgaaat atatcgaact aacagaagaa ggcttcctgg atatggagga
     8641 tgcagcgaaa aagattacgg acaaaaccaa aatcgtctcc attgctcatg tatccaatgt
     8701 cttaggtgtc ttcaatcctg ttcaagaatt agctgaactt gtccatgaaa acggcggggt
     8761 catcgtagta gatggtgctc aatctgcccc gcacatggcc attgatgtcc aagcactaga
     8821 ctgtgatttc tttgctttca gcggacacaa aatgtgcgga ccaacaggta ttggtgtttt
     8881 atatggtaaa cgaaaatggt tagaagaaat ggaacctgtt gaatttggtg gcgaaatgat
     8941 cgattttgtc gaattatacg acagtacatg gaaagaacta ccttggaaat tcgaagcagg
     9001 gacaccaaat atcgccggcg ctatcgcatt aggccatgca atcgattatt tgcaaaaact
     9061 cggtatggac aatatccatc gatatgaaga agaactagcc gcttatgtgt tgccaaaatt
     9121 acaagcaatt gatggattga ctgtttatgg tccacaagac ccgaattttc ggacaggagt
     9181 cgttgcattc aatttggatg ggttacatcc ccatgatgtc gcgacagctt tagacatgga
     9241 aggggtagcc gtcagagcag gtcatcattg tgcgcaaccg ctattgaagt accttgaagt
     9301 atcagcgact gctcgcgcga gcttttattt ctacaacaca acgcaagacg ccgaccgttt
     9361 agttgaagca atcatggcaa caaaggagtt tttccaacat gggactatct aaattagaca
     9421 atttataccg tcaagtgatc ttggatcatt cttcccatcc gcatcatcgt ggaagcttga
     9481 ctgactcgac aagcaagatc gaattgaaca accccacatg tggagatgtg atcgaattgc
     9541 aagtcgatgt aaaagacgga atcatcaaaa atatcgcctt tgacggcagt ggctgctcca
     9601 tcagtacggc aagtgcctcg atgatgacag acgccgtcat cggaaaatca atagaagaag
     9661 cagaacattt aactgtagac tttctacagc ttgtccaagg aaaagaagtc aaagatcccg
     9721 accaattagg cgatgctgct atgcttggtg gtgtagctaa atttcccgct cggatcaaat
     9781 gtgcgacact tgcctggaaa gcactaggaa aagcaatgga aaacgatggt accgcagctt
     9841 tccatcatta ccacaaggaa gaggagtgag aacatgggcg taccagaatt agaagaatat
     9901 aaattcgggt ttcacgatga tgtcgaaccg gttttcagta caggagaagg cttgacagaa
     9961 gaagtgatcc gtgagatgtc aagaatcaaa ggcgaaccag aatggatgct tgaattccgt
    10021 ctgaaatctc tagaaacatt taacaaaatg ccaatgcaaa catggggccc cgacctttca
    10081 gacatcgatt tcgatgccat caaatattac caaaaaccaa gtgacaaacc cgctcgtgac
    10141 tgggaagacg taccagaaaa aatcaaagat acatttgaaa agattggtat accagaagct
    10201 gaacgagctt atctagccgg tgcttccgca caatatgaat cagaagtcgt gtaccataat
    10261 atgaaagatg aattcgaaaa gttaggaatc gtctttactg acactgactc tgctttgaaa
    10321 gaataccctg aattattcaa agagtacttt tcaaaacttg taccgccaac cgacaacaaa
    10381 ttagcagcgc tgaattcagc cgtatggtca ggtgggacct ttatttacgt gccaaaaggc
    10441 gtcaaagtcg atgttccatt acaaacgtac ttccggatca atgcggaaaa cacggggcaa
    10501 ttcgaacgta cgctgatcat tgttgacgaa ggagcaagca tccactatgt cgaaggttgt
    10561 acggcaccta cttattcaag taacagcctg catgccgcca tcgtagaaat ctttacaaga
    10621 aaagatgcgt attgccgtta cacaacgatc caaaactggt cagacaatgt gtacaactta
    10681 gtaaccaaac gagcaaaagc atacgaaggc gcaaccgttg aatggatcga cggaaaccta
    10741 ggtgcaaaaa caacgatgaa atacccaagt gtttacttag atggaaaagg tgcacgaggc
    10801 accatgctct ccatcgcctt tgcaggagca aaccaaatcc aagatacggg tgcaaaaatg
    10861 atccacaatg caccaaatac atctagctct atcgtctcta aatcaatttc aaaagacggc
    10921 ggagaagtaa actatcgagg acaagtaacc tttgccaaaa acagtgcggg ttcgatctct
    10981 catatcgaat gtgacacgat catcatggac gaatggtcga aatccgatac gatcccattc
    11041 aatgagatcc acaacagcca agtctcttta gaacacgaag ccaaagtctc aaaaatctca
    11101 gaagaacaac tctactatct gatgagccga ggcctgaccg aatctgaagc aactgaaatg
    11161 atcgtcatgg gctttgttga accattcacc aaagaacttc caatggaata tgcagtcgaa
    11221 ctcaatcgat tgatcagtta tgagatggaa gggtctgtag gttaataact atttcattgc
    11281 tcgaaacgtt gatattaaag cgtttcgagc gtttttttat ttgtatttta atggttggct
    11341 acgtttttgg ctacgtttgg aattattcgc ccataaattt tgcaaatcgg tcaccagttt
    11401 tcttaacaga ttgtggtgtg acatgagcat agatattcat ggtagtttta atatctttat
    11461 gacctaatcg ttcctgtact tcttttatcg acgcaccact ttcaaacaat aagctacagt
    11521 gggtatgcct aaatccatgt ggagtaattt gaggctccat cggtgtcttt ttgtaaatcc
    11581 agtctaacca atcgtttact acttgggggt agtacaactt gtttgtgttt gtagtgaaaa
    11641 gaaactgttt tggtgaattc gtgttatagc catataagat cattattttc atttgttctt
    11701 gttgccaaat acgcagttgt ttaagtgtaa aatcatcaag ctgaatcgtt cttgatgaat
    11761 ttttagtttt aggtacttgt agaactactt gattaaactc gtcttgagcg atagttttac
    11821 caattgttat tgttttattg aaatagtcta tatcttccca ttgaagcgcc aatgcttcgc
    11881 ttttacgcat tcctgtataa gctaataaac ggaaaaaagt gtacagttta actgaattat
    11941 tttctttagt attctgtaaa aacagttgta attgatcttt cgtgtaaaac ttcaagggtt
    12001 gttcttcttc aatttttctt ggaagcaatg ttttagacat aggattatcc ttacagactt
    12061 ccattgctac tccaaattta aatatttgag cagttgtttt tctcaagaaa ccatattgtt
    12121 ttaatgtttt gtgccattca ttcacctgtt tttggcaata gctaacactt attttatcca
    12181 atcgtaattc cccaaaagca ggtaatatat ggtttttgca aaaacgttga gcaacggcaa
    12241 cagaagacgg ttttactctt gttctgtagt tatcaatcca taattcatac aactcttgaa
    12301 aagttgtaac attgatttta aactcttgtt tttctttaaa gtctacaagt agtctattaa
    12361 gtgctgtttg cgcctcagct tttgatttga atcttctacg ctttgtacgt atatcttttc
    12421 cagtcttggg atctttccct aaataagttt caaatcccca atatttttcg ccattttgta
    12481 atttataact aaaaaatgtt gccatttgat tctctcctta cttcattatt gcaggtagag
    12541 aagttatttg aagaatcaat tatttttaaa tttatcgtga ttatcagtta aaaattttgc
    12601 gattatacta tttttctcat atgggaaatt gtatttttta ctaagatctg aaataaattt
    12661 ttctgtttca aattggtttt tagtaatttt ataaaagaaa tcctcgacag atgaatactt
    12721 tgaaatatct ttataggcta agtccatttc gtctaatagc tgttttaaag aatcaatcat
    12781 tctttctttt tcaccgtatt tgaaatcacc atttcttatc ttgtcttgaa gtttactgtt
    12841 ggctcttttg aaaaatagct ctttagagat agagtgtctt tggttagcgt actcttcaat
    12901 cagaggattg atattattag caagtatctc atttagataa atttcaaaat cttttgagta
    12961 atattttgta aatatatttt ttattatagg attcaataac tcatagtctt tatctagcga
    13021 aaacgtatca ctatatcttt cttgaatgtc tttaaagact ttgtgaggta tttctgggaa
    13081 gtccttaata tatagctcat accctatatc gataaggtat gaagttatat attcttctaa
    13141 agttccccat ttaatccagt caattgttga attagtgata gttgataata atttcagctt
    13201 ttgtggattt ggtaaattgg tcccacgttc ccagttggat atggtcgcac gtggtgaatt
    13261 atctattttc cttccaagtt cttccatagt aaaaccacat gaaaggcgta attgtttgat
    13321 tcgttttcct gcttctgaag gatttggctt catctttttt ctccttttta
//
LOCUS       DACCZY010000079        13304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCZY010000079 DACCZY010000000
VERSION     DACCZY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.080"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            5..2377
                     /locus_tag="IVZ67_001924"
     CDS             5..2377
                     /locus_tag="IVZ67_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8165995.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVZ67_001925"
     CDS             2438..3898
                     /locus_tag="IVZ67_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8165996.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IVZ67_001926"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IVZ67_001926"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8165997.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IVZ67_001927"
     CDS             6353..6946
                     /locus_tag="IVZ67_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8165998.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IVZ67_001928"
     CDS             6959..7858
                     /locus_tag="IVZ67_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8165999.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IVZ67_001929"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IVZ67_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8166000.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IVZ67_001930"
     CDS             8724..8984
                     /locus_tag="IVZ67_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166001.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IVZ67_001931"
     CDS             9429..9635
                     /locus_tag="IVZ67_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8166002.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IVZ67_001932"
     CDS             9683..9886
                     /locus_tag="IVZ67_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166003.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IVZ67_001933"
     CDS             9902..10339
                     /locus_tag="IVZ67_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166004.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="IVZ67_001934"
     CDS             10332..11039
                     /locus_tag="IVZ67_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166005.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="IVZ67_001935"
     CDS             11419..11598
                     /locus_tag="IVZ67_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166006.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="IVZ67_001936"
     CDS             11923..12186
                     /locus_tag="IVZ67_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8166007.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="IVZ67_001937"
     CDS             12180..12530
                     /locus_tag="IVZ67_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8166008.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="IVZ67_001938"
     CDS             complement(12554..13168)
                     /locus_tag="IVZ67_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166009.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4923 a         2198 c         2737 g         3446 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agta
//
LOCUS       DACCZY010000080        13247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCZY010000080 DACCZY010000000
VERSION     DACCZY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.081"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..64
                     /locus_tag="IVZ67_001939"
     CDS             <1..64
                     /locus_tag="IVZ67_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8166010.1"
                     /translation="EKEAMIEENRKKAMRKRVSR"
     gene            complement(126..1937)
                     /locus_tag="IVZ67_001940"
     CDS             complement(126..1937)
                     /locus_tag="IVZ67_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8166011.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1934..2299)
                     /locus_tag="IVZ67_001941"
     CDS             complement(1934..2299)
                     /locus_tag="IVZ67_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8166012.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2462..2857)
                     /locus_tag="IVZ67_001942"
     CDS             complement(2462..2857)
                     /locus_tag="IVZ67_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8166013.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2875..3837)
                     /locus_tag="IVZ67_001943"
     CDS             complement(2875..3837)
                     /locus_tag="IVZ67_001943"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8166014.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3837..4049)
                     /locus_tag="IVZ67_001944"
     CDS             complement(3837..4049)
                     /locus_tag="IVZ67_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8166015.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4070..4768)
                     /locus_tag="IVZ67_001945"
     CDS             complement(4070..4768)
                     /locus_tag="IVZ67_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8166016.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4788..5330)
                     /locus_tag="IVZ67_001946"
     CDS             complement(4788..5330)
                     /locus_tag="IVZ67_001946"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8166017.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5462..6466)
                     /locus_tag="IVZ67_001947"
     CDS             complement(5462..6466)
                     /locus_tag="IVZ67_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8166018.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6463..7452)
                     /locus_tag="IVZ67_001948"
     CDS             complement(6463..7452)
                     /locus_tag="IVZ67_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8166019.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7449..8255)
                     /locus_tag="IVZ67_001949"
     CDS             complement(7449..8255)
                     /locus_tag="IVZ67_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166020.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8421..9377
                     /locus_tag="IVZ67_001950"
     CDS             8421..9377
                     /locus_tag="IVZ67_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8166021.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9454..9972)
                     /locus_tag="IVZ67_001951"
     CDS             complement(9454..9972)
                     /locus_tag="IVZ67_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8166022.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10060..10209)
                     /gene="rpmG"
                     /locus_tag="IVZ67_001952"
     CDS             complement(10060..10209)
                     /gene="rpmG"
                     /locus_tag="IVZ67_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8166023.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10437..10883
                     /locus_tag="IVZ67_001953"
     CDS             10437..10883
                     /locus_tag="IVZ67_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8166024.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11078..12973)
                     /gene="asnB"
                     /locus_tag="IVZ67_001954"
     CDS             complement(11078..12973)
                     /gene="asnB"
                     /locus_tag="IVZ67_001954"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8166025.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4126 a         2815 c         2251 g         4055 t
ORIGIN      
        1 cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg
       61 ttaataatta tttaaaaata cgcatgaaga ctttctaaaa aacagataag tcatcatgcg
      121 tatttttatt tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat
      181 ggatctgtat aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt
      241 ccatagcaat aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct
      301 cgctcaacat ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg
      361 cgaacaagtt tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt
      421 gtttgtggca atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa
      481 ataacatagc ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata
      541 cccgcatcta tcagtgctgc taattctttc gtccgaatga ttggcggacc gatcgttaaa
      601 aagctattta gatgagtaaa ccatccccat agtatttctt tgtattcctt agcagaaaaa
      661 acgtgccatt caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt
      721 gaagcaaatg ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat
      781 attcggctag cttctgaaaa gggctcattc tgataggcaa gaggagtttc taaatacgac
      841 caatcccatc tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg
      901 gagagaaaat catgctcaaa atttttcttt ggaatcggca agtgatattc ttcgatgatt
      961 ttcttgtaat aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc
     1021 aaaaatcccg actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc
     1081 attgctccgc cttgcttttg atttcttcct cgagggtaat aagggagccc ccttcttgat
     1141 ccactgtata caatcggttc cttgcctgat ggatgataga ccagttttcc cttttcgttc
     1201 tcgaagcgcc ctcctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct
     1261 agacctctta aaaagacatt ttctttaggc ggtatcttcg aaagatctac atctgcagga
     1321 ttagaaggaa gctgatacag taaaacattt ttctttgcgt agtctaccca cactctttct
     1381 tcttctgtct catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag
     1441 ctttgctgat ctgtctgtaa aagataacca tcttttcttt tgcttacttt ttttactaaa
     1501 gaataaacaa actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt
     1561 tgatagagtc cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct
     1621 tctataatta gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct
     1681 ttactccatt gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca
     1741 tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc
     1801 aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt
     1861 ctgtcctgat tattatggag aatcctttct gctacactta atcctctagg tccggcacca
     1921 atgatcccta ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat
     1981 ggttctgatg ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa
     2041 gttgactgag tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt
     2101 atttctttgc aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat
     2161 tactaggtgc aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc
     2221 gtggccagaa tctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa
     2281 cacgttttaa tttgatcata gttgtttcct tctttctacc ttttcgctta gcttatatat
     2341 gtagataata gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg
     2401 tgatcttggt caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt
     2461 tttatacttt tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat
     2521 aaatctcttg ataacctgct ttacgtaatt tatttgctgt acggatgctc aaacttttct
     2581 tttgatcgta aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa
     2641 cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc
     2701 gaacatcgat tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg
     2761 ttgctgaacg tttggccatt actcgtagat agacttccca aaggatgatt gcaagcacaa
     2821 tcaatagcaa aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc
     2881 aaatttcaag gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa
     2941 tttgattttt gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac
     3001 acggctacgt aaaaattctc ctgctgccga tactcctcca cctaacacga tacttgacgg
     3061 atttaatgta ttccccaagt ttccgcaagc taaacctaag tagaagcata cgcgatcaac
     3121 aaccatcaat gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac
     3181 ttcttctccg ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa
     3241 gaaacgacct aggcgtacca cacctgtagc gctagataca gtttctaaac atccgcgttt
     3301 accgcaagta cattcgaacc cttcaggatc cacagtgata tgtccgattt ctcctccagc
     3361 tccgctcacg ccgtgaatca aatttccgcc agcaacgata cctccgccaa cacctgttcc
     3421 tagagtaacg aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa
     3481 agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag
     3541 ttttttcaca aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc
     3601 ttcgctgtcc acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat
     3661 attatataaa ttcaaacgat gattgattga ttcaatgatt tcaggaacga tatgcttacc
     3721 atcatccaaa atattggtat cgatgctcca tttttgctga atctctcctt ctggtgttaa
     3781 aattgcaaat tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac
     3841 aaatttcctt tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct
     3901 gtcatgtatg tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc
     3961 attaattcaa tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac
     4021 agctgctgta catcatataa gctctccatc tttaagcact tccttcatat tatacaagta
     4081 aaccatattt ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg
     4141 tagaaagaat cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat
     4201 tccctaacac taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat
     4261 ccagaagacc aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct
     4321 ggatcgctgg gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga
     4381 atatcgaagt acttgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg
     4441 tatttcccat tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg
     4501 cctcgatttg ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc
     4561 caaaatggat gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag
     4621 gaccatacat tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga
     4681 agaccgctgt ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac
     4741 gcaattttat ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac
     4801 aattgttgga tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga
     4861 aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg
     4921 ccaaaaccga ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg
     4981 atcatctccg gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt
     5041 acaagaacgg actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta
     5101 ggtacaacta ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt
     5161 tcaaattcat tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg
     5221 aatagctgtt tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt
     5281 ttcagctttt cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc
     5341 ttccttctct cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata
     5401 aatgcataaa tagaataaaa agaagaccta gaaatgattg gccttccttt tttaatgaat
     5461 ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac
     5521 caaaaagggc aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa
     5581 ccatatctgc actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt
     5641 tattttcttt acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata
     5701 aactacctgt caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt
     5761 tttgactttt agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca
     5821 atacaatcct ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca
     5881 atgcccaaac ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa
     5941 atccataatt ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg
     6001 cattaacggc aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat
     6061 aggccgcaag atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca
     6121 atagaaacgg cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg
     6181 ctccggcatt aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca
     6241 agatataacc agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa
     6301 ctcgaaattc tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa
     6361 aaagctgatc caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga
     6421 caagtacaaa aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct
     6481 tacttgtatc agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc
     6541 aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac
     6601 taaaaaagag acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg
     6661 cggaaccaac agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa
     6721 cactgcaact agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa
     6781 tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa
     6841 aacagcaagt atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc
     6901 tccgcctaca aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg
     6961 gctgatagca gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt
     7021 catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc
     7081 tgccaatgac attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag
     7141 ggccattgcc aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt
     7201 tcttgtcact ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc
     7261 tcccgccaca cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc
     7321 aaaatcaaac aacgcctgcc aaaaatctgc tttttccaca gtcgctgcgc catttgaaag
     7381 agcaacaaaa aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt
     7441 catagtcctc ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact
     7501 ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata
     7561 cactcttttg tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt
     7621 gctaataaat aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc
     7681 gttgtttgtt tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc
     7741 ggatctaaaa atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc
     7801 atagcgatcc agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga
     7861 aatccagcta gacctgtagc atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc
     7921 atgccactga acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc
     7981 atacctgtag ggtgttcact agtttgtgct aataatgcaa ttttttttgc aagttctttt
     8041 gaactgtatg tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt
     8101 atacgggtaa aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta
     8161 gtgattttcc cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag
     8221 ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc
     8281 attctcaatt atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca
     8341 aatgaagcgg ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca
     8401 attatgttag gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat
     8461 tgctgctggc tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa
     8521 ctgccaattc gacgacttca tccaatagta ctcatacagt cacagataca cttggacata
     8581 aagtagagat tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga
     8641 ttgcactagg ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt
     8701 atctgcaaga tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag
     8761 tgttaagctt tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga
     8821 aatacgaaca atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca
     8881 catgggaaga acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa
     8941 aaatcatttc cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa
     9001 tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca
     9061 aaaatcggtc aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag
     9121 tggaagaggt ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag
     9181 ctgaattaga tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca
     9241 atgaaccaag ctggaagaat ctaaaagcag tgacagacaa tcatgtactg caattcggac
     9301 ctgaatctag ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca
     9361 aagaaaaact aaattgattt taactaaaaa aagcatcttc acgattagtc gtgagatgct
     9421 ttttttctta ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt
     9481 ttttactctt tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc
     9541 ctcatactcc tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc
     9601 ttcaaacgtg ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg
     9661 aaaaatccga ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa
     9721 aagttcatcg atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt
     9781 tgtcaacacg atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt
     9841 tcttcccatc actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg
     9901 caatcgccat ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat
     9961 ggaaataaac atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc
    10021 aattctttga ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa
    10081 agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg
    10141 tttgtttttg cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt
    10201 tacgcgcatg tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg
    10261 attatcttac catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa
    10321 ctacttgacg ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata
    10381 aataccctta cacttgttta tatagattat gttttttaat aaagggggat actaatatgc
    10441 catggaacat gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa
    10501 aagcaatcga catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc
    10561 caattgccat tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa
    10621 ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg
    10681 gaaaactgct tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat
    10741 ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag
    10801 gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa
    10861 aagaaaaaga aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca
    10921 acccccttat gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata
    10981 tctataaaac gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt
    11041 aggagaaaac ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg
    11101 cttctgcttg cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga
    11161 agattgtcca gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact
    11221 tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga
    11281 cttgttcgta gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt
    11341 tcacacgatt cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt
    11401 ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt
    11461 cgatatccaa taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct
    11521 ttaacaagat atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt
    11581 catctttgat tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg
    11641 ctgtttgata ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat
    11701 gaccgataaa atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga
    11761 agtttttcat ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt
    11821 tcaatccttg agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt
    11881 atcccgcaaa cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag
    11941 caagatttgc taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt
    12001 gatactggat caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat
    12061 tatctaagtc caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat
    12121 catcaaaccc gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat
    12181 caaccccgct ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag
    12241 aagcttgaac ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg
    12301 tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt tccatcttta tacgtataat
    12361 aatggccttc aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat
    12421 attggaaagt catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat
    12481 gaggcagaaa acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat
    12541 aatatggttt gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc
    12601 aaatagcaaa agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt
    12661 aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca
    12721 attcttctct caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc
    12781 gatcttcatt gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga
    12841 atcctaaagc aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga
    12901 ttcgatccat cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta
    12961 caataccgca catttaactt tcacatccta atataaaatt actgtttatt tcattcatat
    13021 gcatcttatc atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg
    13081 aaaacaaaag atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc
    13141 atgttttctt ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct
    13201 atttccaaaa aaaccagcaa aaaaagacac gacctaaaga tcgtgtc
//
LOCUS       DACCZY010000081        13185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCZY010000081 DACCZY010000000
VERSION     DACCZY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.082"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(236..308)
                     /locus_tag="IVZ67_001955"
     tRNA            complement(236..308)
                     /locus_tag="IVZ67_001955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Lys,seq:ctt)
     gene            689..1258
                     /locus_tag="IVZ67_001956"
     CDS             689..1258
                     /locus_tag="IVZ67_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8166026.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1245..2105
                     /locus_tag="IVZ67_001957"
     CDS             1245..2105
                     /locus_tag="IVZ67_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8166027.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2295..2999
                     /locus_tag="IVZ67_001958"
     CDS             2295..2999
                     /locus_tag="IVZ67_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166028.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3004..4839
                     /gene="walK"
                     /locus_tag="IVZ67_001959"
     CDS             3004..4839
                     /gene="walK"
                     /locus_tag="IVZ67_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8166029.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4836..6152
                     /locus_tag="IVZ67_001960"
     CDS             4836..6152
                     /locus_tag="IVZ67_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166030.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6153..7022
                     /locus_tag="IVZ67_001961"
     CDS             6153..7022
                     /locus_tag="IVZ67_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166031.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7077..7886
                     /locus_tag="IVZ67_001962"
     CDS             7077..7886
                     /locus_tag="IVZ67_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8166032.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7989..8618)
                     /locus_tag="IVZ67_001963"
     CDS             complement(7989..8618)
                     /locus_tag="IVZ67_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8166033.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8788..10080
                     /locus_tag="IVZ67_001964"
     CDS             8788..10080
                     /locus_tag="IVZ67_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166034.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10156..10818)
                     /locus_tag="IVZ67_001965"
     CDS             complement(10156..10818)
                     /locus_tag="IVZ67_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8166035.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11043..11327
                     /locus_tag="IVZ67_001966"
     CDS             11043..11327
                     /locus_tag="IVZ67_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8166036.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11378..13003
                     /gene="groL"
                     /locus_tag="IVZ67_001967"
     CDS             11378..13003
                     /gene="groL"
                     /locus_tag="IVZ67_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8166037.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4376 a         2274 c         2847 g         3688 t
ORIGIN      
        1 tgatgaaaat atgcttctat cattacaagc tcgtttggtg taagatgggt ataagtcatt
       61 tatgttcact ctccttgtat gctttagcgg gtattacaat ttgagtgtaa cataaatggc
      121 ttttttattt gtctcgctta attatacaaa cggcgaaaat aaaaaaagaa cagcgtaaat
      181 gttttcgaaa tgctttcgca ccaacattta cctgttccgt gggtttggca cccaatgggc
      241 cgtgaggggc tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct
      301 aacggcccgt acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact
      361 acatttaaag aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt
      421 cacatttgct cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac
      481 cctaacactt ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc
      541 ttaacgataa aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct
      601 aatcaaatca cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt
      661 ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg
      721 ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact
      781 ttatatggac caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga
      841 aaagcatcca ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc
      901 agcaccagtt aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac
      961 gttgaaacaa gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac
     1021 agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat
     1081 ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt
     1141 agaatatatt gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa
     1201 aacgattgtt ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa
     1261 tcgcaatttt agtagattca ggtacggatg tgccagaaga attgataaaa aaatacgaga
     1321 tgtatatgat cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca
     1381 ttacctctga agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg
     1441 atggtgagtc gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac
     1501 ttgcggtaac aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac
     1561 aacagcaaga agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag
     1621 gtttttctgc gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga
     1681 tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg
     1741 aatatcttca aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa
     1801 agcttaaccc aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta
     1861 ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg
     1921 caaaggtctt caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg
     1981 aagcgagact taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc
     2041 cggcattagt agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag
     2101 gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt
     2161 tagaacaaaa aataataaag tattttgcac tttcatttta attttctctt ttgttttata
     2221 ggcaaagatg aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata
     2281 agaggtggct ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata
     2341 tcgtcaaatt caatttagcc aaagaaggct atgatgttta tactgcatat gatggggaag
     2401 aagccttgga aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac
     2461 ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca
     2521 ttatggtaac agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg
     2581 atgattacgt gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc
     2641 ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga
     2701 ttggtgattt gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg
     2761 agttaacaca tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga
     2821 tgacaagaga acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa
     2881 cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa
     2941 attatttagt gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat
     3001 tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt
     3061 attctgattt tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa
     3121 cgttccacga tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca
     3181 acaatcagcc cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat
     3241 atcaaaagat ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac
     3301 gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa
     3361 aatgattatg ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac
     3421 gataaacggg tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc
     3481 ggggctcttt acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca
     3541 gtcattttct ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta
     3601 gttgctcgtt cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct
     3661 agaggagact acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct
     3721 gataccttca atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa
     3781 cgcaatcgtt tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc
     3841 aggggtaaag tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag
     3901 gaagcaatcg gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg
     3961 aaattattag aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt
     4021 gatcagatga tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc
     4081 ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt
     4141 gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat
     4201 atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag
     4261 gtaacgctag atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct
     4321 cgcatggata caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg
     4381 aattttgttc ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc
     4441 cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa
     4501 gtagtcgata atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact
     4561 tgtcgcttat tagaaacgca taataacgtg gttttaagta tcaccgatca aggattgggg
     4621 attcctaaaa aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt
     4681 gcaagagcac aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg
     4741 catcgaggcg ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg
     4801 ctgccatatg aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc
     4861 gtataggact cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga
     4921 gcccagcagg aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc
     4981 aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg
     5041 gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata
     5101 aagcaagatt tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg
     5161 tgaaaaatct gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat
     5221 acaaggaagc atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata
     5281 tgtattttac tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt
     5341 cgaaccatct cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat
     5401 taacagcaaa agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc
     5461 aatatgctac gaaacattcg atcaaactga aaaagtatag ttatatcctg tcacagcaac
     5521 cctatacact ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg
     5581 aaagcaatga attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc
     5641 aagtagttga ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg
     5701 aaagtttccc ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc
     5761 gcagtgacaa tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag
     5821 acagcagagg acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga
     5881 atatccaaac cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac
     5941 ttgcaagtac gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga
     6001 ttggttcagt gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg
     6061 atctcttacc tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc
     6121 tggaaaaatt acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat
     6181 ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat
     6241 accaagaaaa caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta
     6301 gcgacgatat cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta
     6361 gcgcggagca gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg
     6421 gcttgctggc aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt
     6481 cagatgctga aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt
     6541 tatctaacaa agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc
     6601 atttagaaga agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca
     6661 atgatgatac agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca
     6721 cgaaatacat gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt
     6781 atacggaaga ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa
     6841 aaattttatg gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt
     6901 atgttcctgt ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagtg
     6961 tcaacgcttt cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat
     7021 aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg
     7081 ctgaagaaaa accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac
     7141 tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa
     7201 tcacttcctt gatggctcaa attgatcgga caccggacca attagatgga atacttgtga
     7261 cgcatgaaca tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg
     7321 atgtttttgc caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag
     7381 tggaacaaaa acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg
     7441 agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa
     7501 atcggtcatt cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca
     7561 ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg
     7621 gaccttatcc gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg
     7681 aagacggtgc acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg
     7741 gccatcttag caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt
     7801 tgaaagaaaa agattttggc gttggacatg attttgaaat ctatgataca gatccagatg
     7861 aagcaactga gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag
     7921 ataaaatcaa tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa
     7981 taaccgtatt atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc
     8041 tcatctacta atgcaatcgc gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt
     8101 ctttgtaaat aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat
     8161 aaagaagctg gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat
     8221 ccttcgtaca tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc
     8281 atacgagtcg tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag
     8341 cgtgtatcgg ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct
     8401 tcttttcctt tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta
     8461 gttgtcaact caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga
     8521 tgacgcacga ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa
     8581 cctgcatggc cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc
     8641 tgtccattgt aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt
     8701 gcgcattcct ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat
     8761 aaaaatagaa gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca
     8821 atgataacat ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac
     8881 acgatcccaa taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat
     8941 tacatcgctc agtggataga caaaacagga ttttctatgt attttacaca agtataccag
     9001 gctacatttg atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa
     9061 gaagaagaca tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt
     9121 attcagccac taaatactca gatagatact gtctggacag gtgctgaagg gatactagga
     9181 gcagttacaa ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa
     9241 gggtggaagc aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt
     9301 ccaatcattg ttatagtcac ttttacgctt atcggttcag cggtttttca acagttgttc
     9361 agaatgactg ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata
     9421 gacacgttgt ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt
     9481 cttcatggat ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta
     9541 gcgaatgcag aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc
     9601 gttactcagc aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg
     9661 gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt
     9721 gctcttttac cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg
     9781 atgaacccga tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc
     9841 tattccgctt tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca
     9901 acaccgccga tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt
     9961 atcgtgttgt caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca
    10021 aactgcaaaa aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa
    10081 aaaacaaaaa tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag
    10141 tatttatagt catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa
    10201 tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc
    10261 taggctgaag aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa
    10321 cgcaaacagc aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat
    10381 ccccagtata ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc
    10441 caaaataaaa gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg
    10501 ggttgctttg ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc
    10561 gacaaagatt ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc
    10621 tgattcgatc gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc
    10681 tagtatatac atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat
    10741 cgaagctagg atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct
    10801 atattttttt aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc
    10861 ttatatcgat tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt
    10921 cttttctttg atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag
    10981 gttagcactc gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta
    11041 tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa
    11101 ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg
    11161 ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag
    11221 caggcgacca agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag
    11281 aatatctgat cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa
    11341 aaaaataatc aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc
    11401 agaagacgca cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt
    11461 aacattagga cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat
    11521 cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat
    11581 gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac
    11641 aacgacagca actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc
    11701 aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga
    11761 agaattgcat aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc
    11821 tgtttcttcg ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg
    11881 aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt
    11941 agaaggtatg cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa
    12001 aatggaagct gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat
    12061 ccaagatatt ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat
    12121 tgctgacgat gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac
    12181 attcaacgta gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga
    12241 agatatcgct attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa
    12301 agatgtaaca atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac
    12361 aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa
    12421 aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc
    12481 taaattagca ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa
    12541 agaattaaaa ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg
    12601 aatggtctca ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga
    12661 agcagaagga gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat
    12721 tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa
    12781 cgtggaacta ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc
    12841 aggtatcgta gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc
    12901 tgccttgtta ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc
    12961 agctcctgca atggatccat caatgatggg cggtatgatg taagaaaaag caacttgctt
    13021 atctacaact gatcattagt gaaagacaga taaaaagagg ctggaacaga agcgtttaac
    13081 tccaagaaat aagaagaaat tcacggaaat tgcttttcaa atttttgtga atttcagctt
    13141 atttccgaag gagttgcttc tgcttccgct gtttatacgt gttta
//
LOCUS       DACCZY010000082        13176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCZY010000082 DACCZY010000000
VERSION     DACCZY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.083"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(58..1320)
                     /locus_tag="IVZ67_001968"
     CDS             complement(58..1320)
                     /locus_tag="IVZ67_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166038.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1313..2212)
                     /locus_tag="IVZ67_001969"
     CDS             complement(1313..2212)
                     /locus_tag="IVZ67_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166039.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2357..4135)
                     /gene="uvrC"
                     /locus_tag="IVZ67_001970"
     CDS             complement(2357..4135)
                     /gene="uvrC"
                     /locus_tag="IVZ67_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8166040.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4317..4631)
                     /gene="trxA"
                     /locus_tag="IVZ67_001971"
     CDS             complement(4317..4631)
                     /gene="trxA"
                     /locus_tag="IVZ67_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8166041.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4718..7078)
                     /locus_tag="IVZ67_001972"
     CDS             complement(4718..7078)
                     /locus_tag="IVZ67_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8166042.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7193..7741)
                     /locus_tag="IVZ67_001973"
     CDS             complement(7193..7741)
                     /locus_tag="IVZ67_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8166043.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7800..8237)
                     /gene="zapA"
                     /locus_tag="IVZ67_001974"
     CDS             complement(7800..8237)
                     /gene="zapA"
                     /locus_tag="IVZ67_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8166044.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8439..9356
                     /locus_tag="IVZ67_001975"
     CDS             8439..9356
                     /locus_tag="IVZ67_001975"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8166045.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9444..10355)
                     /locus_tag="IVZ67_001976"
     CDS             complement(9444..10355)
                     /locus_tag="IVZ67_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8166046.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10756..11334)
                     /locus_tag="IVZ67_001977"
     CDS             complement(10756..11334)
                     /locus_tag="IVZ67_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8166047.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11354..12022)
                     /locus_tag="IVZ67_001978"
     CDS             complement(11354..12022)
                     /locus_tag="IVZ67_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166048.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12037..13107)
                     /locus_tag="IVZ67_001979"
     CDS             complement(12037..13107)
                     /locus_tag="IVZ67_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166049.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3744 a         2827 c         2170 g         4435 t
ORIGIN      
        1 acaaaaagaa gctgggaaca atagatgtcc agcttccttc tgtattttag gattgtttta
       61 tgcttttttt cgttcattcc tcataattga aatcgattgt ttcaatgaac tccataatcc
      121 gccttcgcta taaacgagta cattcgattt gtacatgttt gcagacaaga tcatcaataa
      181 gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac cgactgtctc
      241 atttgctaag cgaaccggca taatatatga agataagaat ggaatatacg aagtgatccg
      301 aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatgt atccgcctag
      361 agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt tgaccaaaga
      421 gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga atagaagaga
      481 ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata tatttttgac
      541 catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt ataatgccat
      601 ttgcgttaat gcaacaagta aaacacctgt taattttccg taaaaatgct tttgtgcagt
      661 cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga tctcttgtgc
      721 aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta cataacttac
      781 gacgtattgg actgtcgagt tatcttctcc gactgtcatt tttccttttg aatcaaaatt
      841 gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt ctggtgaaat
      901 gcctaattga ctcgctcgaa gcatcgattg caagccagtc agttgttgtt gtatcagtag
      961 ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct tatcattctc
     1021 agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt gtgctttttc
     1081 ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca tttgctgact
     1141 gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt gttgagcaaa
     1201 gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca tgatcaagaa
     1261 agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa acttacgcat
     1321 cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc tggttaaaca
     1381 taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct tcaggatctt
     1441 ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg ccttcgattt
     1501 cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg ccaaatgatt
     1561 cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac atgatcaaat
     1621 gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca catgaaccat
     1681 gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga tctaacccgc
     1741 tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg atcagctgga
     1801 ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc ccttttacct
     1861 gaaatttttc catccaaaag tcgatttttg gttcaatctc ttttcttgat ttccctctaa
     1921 gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt ccccgttcct
     1981 ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca ttccataaca
     2041 cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt gtcttcccag
     2101 ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa gagacatgat
     2161 cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac atttcttcac
     2221 tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt tattatttac
     2281 caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt tactttcttt
     2341 gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt gcgacatttt
     2401 tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt ttcaacgatt
     2461 tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt tcatctaatc
     2521 gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg aatcgatgga
     2581 cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca agcggtacta
     2641 cagccaaatc tgtaccgaat agcaactcat ttgttttatg tttatcgttt tttgccatgc
     2701 cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc acatctactt
     2761 gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg cgaatcactc
     2821 gcgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt ccgttgactg
     2881 tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg aatacgacca
     2941 tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg atacgtattg
     3001 gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc cgttcctgtt
     3061 ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta ctcgccaatt
     3121 caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct aacaaagctt
     3181 ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg aaatgctcat
     3241 tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg ttatagaaag
     3301 gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac acttggacac
     3361 acatccatcc tttatcaatc gcataaccaa atacatctcg atccagtaaa tcagtatttg
     3421 tcattttttg gcgagtcatg attgtctcaa tggcacggat ctggtctcgg tattctgcag
     3481 ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc tctgactcga
     3541 ttttttcgtg tcctccgttt aaaaagtttt tcacttcatc aacgattttc gtatagactt
     3601 tcggatctac gtcgaaataa taaggacaca gacattgtcc caaatgatag taaagacaag
     3661 gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg aggatcttct
     3721 ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct ttgtctttta
     3781 gtaccttacg agtaatcacc aaacggggat atttttcatt cgttatctta aggaaaggat
     3841 aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt atcagattga
     3901 tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag tcttcgattt
     3961 cactaacaag ccgttccgtt ttcgtattat ggctaccagt aaaatatgag cggacacgat
     4021 tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct ttcatcaagt
     4081 aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg ttcatctggt
     4141 actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa caatccagcc
     4201 tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca accgcaaaaa
     4261 aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt aagtttttat
     4321 aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac agctttttct
     4381 actacttgac catctttttt caacattaat gttggaatgc tcatgatacc gaattgttga
     4441 ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc ttcatcatat
     4501 tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca ccaagttgcc
     4561 caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt atctgtaatt
     4621 acttgtgaca tatcaaagac ctccattttt aaaatctatg actagtatag catttctata
     4681 cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta acaattgtcg
     4741 caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc cgatggtttt
     4801 taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg acaatagtca
     4861 cttgaggata accagctaga atggcagaat ccaaatattg atctacctca cttagcgctt
     4921 cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta gattcagatc
     4981 gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc gtcagctcgt
     5041 cttcggacac attcattttt aatataccga tttctacttg ccactgattc ccgtttttac
     5101 gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc ggtttcaacg
     5161 ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct tcttgatgta
     5221 gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt ccttgcccaa
     5281 tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct tccgcctctg
     5341 acacgacttc atttgctttt ttctttgctt tttccatttc tttttcacgt tcttcaaaga
     5401 aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa acaaaatgac
     5461 gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct gtgatcattt
     5521 cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata acttctgtat
     5581 caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg ccaatcagca
     5641 aacgatacgt tggacttaat gtatccacat caaactccat gctggcatta atcgtatttg
     5701 cccgattata tccataaact ttcaattcag gataatgcgt tgttgccatc acataagcag
     5761 actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct tgcggatctg
     5821 ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt gataaaatat
     5881 ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc gactgttcgt
     5941 ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca cctgcaggaa
     6001 tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac gtgatcgttt
     6061 tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg attgtgatgt
     6121 cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc aagacgacat
     6181 gattgtcttc actgattccc ggcacgatag cttttacttc tttaccaaat cgtgcttttg
     6241 cattcatcag atccatttta ccaatcacat aagcattgtg gatgatttct tgacgatggg
     6301 gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt tcagcgattt
     6361 ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata aataaagtct
     6421 gtcctgaagc actttgatcg tggactacac cgccaaatac accgcggtat tcttgtttga
     6481 ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa tactttgcat
     6541 ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt cgccgaatgt
     6601 tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg tcttcatcga
     6661 ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct gcccacatat
     6721 acaaacgtgc aaattcgatt tcactgtcat aaagatcatc aatgaaacga ttcaattcgc
     6781 ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag tctgctccga
     6841 tctcaatccg tttcatatgg ggacggatat tttcaagttt aggtataggg atcccgcctc
     6901 gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg atcgtttgtt
     6961 cctcgctcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc actacaaatt
     7021 gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg ttattcattt
     7081 tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta cgcagccttc
     7141 ttaaataatc tactctgtac aaaaaacgaa atattttcca gcactatttt ccttaattca
     7201 aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt ctctcaacga
     7261 taaagcgagc gaaactatgc tcgccgaatt gattttggac cacatctaat ggaacaaagg
     7321 caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac aggatgcctc
     7381 ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg acaaaaaccc
     7441 ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca gttcctgcat
     7501 aaaaagcttc atctaggtga aaggcaaata cctgatcaaa aaatgctaac tttgatgttg
     7561 gcgtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt ttgtaaaaat
     7621 gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc aaagcaaacc
     7681 cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc aaactcagca
     7741 tgctacgttc ctttctcttc taactttttt taaatttttc ttattttgat aaaaggatct
     7801 taattagaag atttttgttt aatttgtcct tttcgtttct cgtttaaaat ctgttgtgct
     7861 tccacatgat ttttaatggt atgatctgct tgaccatttt ctttcaatac ttcccgagct
     7921 tcgttctcga ttgcttcaat gcgtttcaca cgattttcaa gctctgtaaa tttgatcatt
     7981 tttttcttta attctgctgt ttcttcttcc agctccaaga ttctttcttg tttcttcaat
     8041 tgatccgaaa gcgcattgac agccatcaag attgctgctt gttcattatc catttgtggt
     8101 gataattgtt tcaattctgc taactgttca ttgatcagct tcgttacgat atccatatga
     8161 tgcttggttt cccgtccgat aatagtatat gtttggttgg caatcaccgc tttgtagcgt
     8221 gttttttcgt gcgccatacg aatcatttcc tcctatttga tgaaagtttt atttagtgta
     8281 ccatgaagtt ggttctttca ccgttctcca caaatatttt tcacatactt aactattata
     8341 tgataaactt taaaaaaatg ctatgtctcg caatctctta gaaaacgaaa agtcgtgagt
     8401 tgcggtatag ttttatgtat aaaaacgagg agaattttat gagcaacgtc attatcaaag
     8461 caacaaacga ccaacttagt caaatgaaag catactatcg ctcttatttg ttaaataaac
     8521 agattcctta tactagcttt gctgcaaaaa aagacgggac gaccattacc gcttatacat
     8581 ccggaaaagt gatgtttcaa ggtacgaatg cagcaataga agctgataaa tggggggagg
     8641 cacaggataa acaagccaag ccagcaaaaa cagcctcttc tttgccaaaa aatatcagtc
     8701 aattatccgt tttaggtagt gatgaggtcg gaaatggcag ctatttcggt ccgctaacag
     8761 tatgtgctgc ttatgtgaaa cgtgatcagc ttgcagaatt acgtaaactt ggtgttagag
     8821 attcaaaaga gttaaaagat agccaaatca tccagcttgc tgacatctta aaaaaaacca
     8881 tcccctataa actgcttgtc ttatctccag aaaaatacaa tgagatccaa ccaaaataca
     8941 atgccgttcg gatgaaagtc gctttgcaca atcaagcgat ccatttgctg ctaaaaaaaa
     9001 tcgagccaga aaaaccagaa gcgattttga tcgatcagtt tacgccggaa gcaaatttta
     9061 aaaaatatgc gcgtttagaa aaaaatcaat tacaagaaaa actgtacttc gttactaaag
     9121 gagaacaata tcatttagct gttgctgctg cttctatcat cagtcgtgcc agctttttgg
     9181 aagagttaca caaagcatca gctgaagctg gaatcaccct tccatcagga gctggaacga
     9241 aatctgacca agtagcggca aaactattgg agaaaggcgg catgcctatg cttgaaaaat
     9301 atgccaaact tcactttgca aatacagaaa aagctttgaa gctactaaaa aaataaacgg
     9361 tgttcaaaag cttgcatctg cgataataag cccggccaaa cgaaaaatat gaggagctat
     9421 ttttgtttgg ctgggcttat ttattactga aaataagtgg tcaatgcttg tgtcaaactt
     9481 gcagcaatct gctgctgata actgtctgtt actaattcct ttaaatcact atcattattc
     9541 atataaccca tctcgattaa aatagaaggt tgaagatttt ctcgaagaac ttgatagttc
     9601 ccagtggaga ttcctcttga atccagaggc agatcagtga aggagtcagc aattgtctgt
     9661 gctaatgttt cctcttgtcc atagtaataa tatgtcgtga ttccagttgc ttcattcgca
     9721 tattccgtag agtctgcgtg taaactgata aagacatctg cctttttttc ttcactgtag
     9781 tcgcatatat ctccaagctg tactaattca tctgtagtat gggtcaaata aaccgttgct
     9841 ccagcatcct ctagcgcctt ttttactttt atggctgtac ttaatgttat atctttttct
     9901 tctgtttgtt catcattgct taatgcccca gtatcatttc cgccatgccc aggatctaat
     9961 acaatcactg catcagatag ctcttccggt tttgtttgta tattctttgt gactttggtt
    10021 ccatcttcga gggctaaata tccagtatag ccatcatttg tcttgatctg ataagcattt
    10081 tttgtttcag aaagaaactc cgccagctct ccgccgttta tagttgcaag tggggtagtt
    10141 agcgagtcat ctgtatatag tgtacggtca ttttttccga ttttgattaa tgtctcttgt
    10201 tctgtatcat caaatgattc ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt
    10261 tgtccaacta tctttttttg accaaaaaca tctatttgaa aacagataat aatgataatg
    10321 attgcaaata caaaagctcc aattattttt ttcattcttg actctcacct cgtagatcca
    10381 gactaagggc caaaacttaa aatcagctta agtaagcaaa aaaggggctg taaaagatat
    10441 aaccgtagaa aataacatta ctgctaaaat gatttttttc atttttggca tatcctgaaa
    10501 tctccttttg actattcgtg gtgattcatc tacccaataa atagtttcct tcattctaca
    10561 aacaattaat ttcttcgtta ttaaattaaa acacttcctg gaacaccgac ataataaata
    10621 aaaatatacc ctgataatca tgagagaata acaaatttgt ctttatgaaa aactacctat
    10681 ataaagaagc aagatataac gatcttcgtc atatcttgct tcttcaccta actattttct
    10741 ccctatataa cagctttacg cttctcctaa tacaccattc aaatataaat cgatcaattg
    10801 cgccacattt agcggttctt ccacaggtct tttcgaccga gtgatgtaag gtgctaacac
    10861 taagaaaaaa tgccttgctg cgatttcttt tgaaatccca tttcttagca cagattggtt
    10921 tgctgaaaag atttcttcaa aaggttcacg ataatttttc ttccatagaa agaaaagatg
    10981 atctcttgtt tcctttgaaa gattgctttc aagatcgtga agcatcagaa acaaatcgac
    11041 cagatgtgtc tcaatcaaaa actgcgccat ttttgtcaag gttacttgaa atcccgtttc
    11101 gcttgtttga acgatttctc tcaaagcttt tccaacatct tttaagtggt tttctaaaac
    11161 tgccgtatat agtttttctt tatcaccgaa ataatgatag agattgggct gggtgatccc
    11221 aactttttgt gcgatttctc ttgttgaagt gccgagatat cccttggaca taaacaagtc
    11281 agtcgcttct ttcaaaattt tttgctgtgt atctttgttg tatcgatgga ccatttttcc
    11341 acctgctttt tggttattct ttttctttca atacaccatc ttccatcata aatattctat
    11401 cgcatttgtc aatcagccgt gtatcgtgag taaccatgat cgttgcttta tttttgtctt
    11461 ttgattcttt tgccaagata tcgactactt ctcgtgcctt ttctgaatcc aaactagcag
    11521 ttggttcatc tgctagaata atggacggat cattgtacaa tgcacgagcg attgcgaccc
    11581 tttgccgttc tccaccagat agttcttctg gatacttgtt gatcaactta tccacgccaa
    11641 gctgctcaaa taattcgttt gcttcttttt gacgatgttc atgtttaatc ttatctacta
    11701 ataataactg cttttttact gtcagaaaag gaaccagatt ggaagcttgc aaaataaagc
    11761 caatttcttt aaaacgtagt ttaacgcgtt cgctttcttt ttgattactg aattcttgat
    11821 cattgataaa tacagagcca ctgctaggcg tctgcaatcc gcctgccaaa gtcaaaaaag
    11881 tacttttacc tgaaccagaa ggaccgatga ttgcaacaaa ttccccttta tcgatactca
    11941 tggttgtttc tttcaatgct ttaactactt ggtcgccatc ttggaaactt ttttctactt
    12001 ctttaaattc tattgctttc atgttcttcc tccttcttat ccaattgcct tcaacggatc
    12061 gatttttacg attgtccgaa cagaaaatag tgcgcctaac acagctacta ctaacatcaa
    12121 caagctaatg acgccaaaaa acagccaatt gctttggaat ggaaccgcat caggtagaac
    12181 aagggatgtt cccactgttc ctaatagacc aagaagaata ccaacaaatg ataaaatgaa
    12241 tgtttgtgcc actacagaac gcgcaataaa tcctcctgtg atcccttgtg ctttcattac
    12301 accaaagata ttaatctttt gcatcgtcag tacataaatg aaaatcccga tcacaatagc
    12361 cgcaataacg atcaaaaatc cgatcataaa tccaaaagtc aatacttgcg cattatatcc
    12421 aggtaattca ttgatgaatt tagaaatttt gatctgttct aaatcttcag gtaaattact
    12481 gattttacct ctgacaacta ttgcgttaat ccgtgcattt tcacttgtat cttctttttc
    12541 gaagcggatt tcttggtaag cattgatggt tgtatagaga actggtgaga cattgaattt
    12601 ggcatgatcc gtaaagccag ttagtttaaa cgtcttgtca ctaccagata acttaactgt
    12661 atctcctatg gctagaccat attcttcttt caaactaata tcgcctactg cctcatcatc
    12721 gttatcaaaa gcttttcctt caacaagatt gggcatgatg aattgatttt tatcgatacc
    12781 gaagaagcta acattgattt ttccttcttc tgtactgtct ttactagtca cgactccagg
    12841 tgtttgggca agatacgcca ctttcccacc ttcgacttct tctgctatct tcttagtaat
    12901 catggacatc cctagatttg aatttgcatc tttcgaaagc acaatcgaat ctgcctccca
    12961 cttgtcgact gctgttcgat tatcttgagc taagccataa gctaacccag tcaaaaaaaa
    13021 taccaggtag gcgatcaaaa acatcacccc tgcaactaac gcataacgta actttgaatg
    13081 cataatttca tttaatgcta aaaacattta actcctcctc ctgaaataat cattttatac
    13141 aacaatttta tcacttgata aatttttggc gaatat
//
LOCUS       DACCZY010000083        12895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCZY010000083 DACCZY010000000
VERSION     DACCZY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.084"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            138..1424
                     /gene="efmA"
                     /locus_tag="IVZ67_001980"
     CDS             138..1424
                     /gene="efmA"
                     /locus_tag="IVZ67_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8166050.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1562..2161)
                     /locus_tag="IVZ67_001981"
     CDS             complement(1562..2161)
                     /locus_tag="IVZ67_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8166051.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2565..3686
                     /gene="mnmA"
                     /locus_tag="IVZ67_001982"
     CDS             2565..3686
                     /gene="mnmA"
                     /locus_tag="IVZ67_001982"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8166052.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3932..5353
                     /gene="arcD"
                     /locus_tag="IVZ67_001983"
     CDS             3932..5353
                     /gene="arcD"
                     /locus_tag="IVZ67_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8166053.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5367..6485
                     /locus_tag="IVZ67_001984"
     CDS             5367..6485
                     /locus_tag="IVZ67_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8166054.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6545..7837)
                     /locus_tag="IVZ67_001985"
     CDS             complement(6545..7837)
                     /locus_tag="IVZ67_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8166055.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7987..8547
                     /locus_tag="IVZ67_001986"
     CDS             7987..8547
                     /locus_tag="IVZ67_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8166056.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8676..9053
                     /locus_tag="IVZ67_001987"
     CDS             8676..9053
                     /locus_tag="IVZ67_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8166057.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8986..11058
                     /locus_tag="IVZ67_001988"
     CDS             8986..11058
                     /locus_tag="IVZ67_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8166058.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11269..12216)
                     /locus_tag="IVZ67_001989"
     CDS             complement(11269..12216)
                     /locus_tag="IVZ67_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8166059.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12462..>12895
                     /locus_tag="IVZ67_001990"
     CDS             12462..>12895
                     /locus_tag="IVZ67_001990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8166060.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4158 a         2339 c         2748 g         3650 t
ORIGIN      
        1 atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt
       61 attttttgaa aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa
      121 agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa
      181 attatttgtt ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat
      241 atgcgatcat ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta
      301 ctttactagg aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc
      361 gctgggataa aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta
      421 ttttagcagt agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt
      481 tgtttatgcg ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga
      541 caatcgttcc atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag
      601 cgaatttcat cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt
      661 accttatttt attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga
      721 agatcccaaa agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa
      781 aatttgggct gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg
      841 catttgtgat gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt
      901 actttggtgg ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc
      961 tcttaggcgg tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta
     1021 ttatagccta catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata
     1081 gtcaagggtt cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt
     1141 tcaatacact gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg
     1201 tcttagggaa cttcaactct ttgatgaacc tggcaggtcc aattggatta ttatttgcag
     1261 gaccattagc tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt
     1321 tattatgcgg aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac
     1381 agaaaaaact ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt
     1441 gacagaagtc ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac
     1501 tcaaatgaaa aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt
     1561 ttcacgacct atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata
     1621 acggagtgcc aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta
     1681 cattgacatc gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga
     1741 tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga
     1801 atttaacgat ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag
     1861 gatcattttt ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt
     1921 ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt
     1981 gcggatgcat agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat
     2041 tccagctagt gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa
     2101 agtcgttttt tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca
     2161 tgtatctttt gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat
     2221 aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt
     2281 ttgaaaaata gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt
     2341 tttatgagta tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga
     2401 ctatcatgtt tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta
     2461 agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc
     2521 aactcgtaga tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca
     2581 aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt
     2641 taaaggaaca aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg
     2701 aaaacggtgt atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa
     2761 tcgggattcc ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat
     2821 atttcctagc cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag
     2881 aaatcaaatt caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta
     2941 ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag
     3001 tagacaataa taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa
     3061 aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg
     3121 caggccttgc gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga
     3181 acttcaaaca atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg
     3241 atggtgaagt caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag
     3301 gactagggat cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc
     3361 tagcgacaaa cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa
     3421 gcttggatgc aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt
     3481 gtacagcgaa attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg
     3541 ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag
     3601 ttgtatttta tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag
     3661 aaacaaaagt tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa
     3721 caaaagaaac accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt
     3781 tattcatttt atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata
     3841 aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat
     3901 ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa
     3961 atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac
     4021 aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg
     4081 gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct
     4141 agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag
     4201 tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat
     4261 gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt
     4321 atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc
     4381 gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat
     4441 atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc
     4501 tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg
     4561 tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg
     4621 cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt
     4681 tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac
     4741 tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc
     4801 gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct
     4861 cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa
     4921 aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct
     4981 attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac
     5041 agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa
     5101 agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg
     5161 catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt
     5221 ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt
     5281 aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg
     5341 gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc
     5401 taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga
     5461 cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca
     5521 aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg
     5581 cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg
     5641 ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc
     5701 ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc
     5761 atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac
     5821 ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc
     5881 ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag
     5941 atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg
     6001 aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg
     6061 gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga
     6121 tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa
     6181 aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg
     6241 tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta
     6301 aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag
     6361 tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt
     6421 gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg
     6481 cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt
     6541 acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt
     6601 cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga
     6661 ccaaaacaag aagagaagtc gaaagaaaga cgccgcgata accaaatgca ctggaaacac
     6721 tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg ttataactga
     6781 agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga
     6841 gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg
     6901 tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc
     6961 tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa
     7021 tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca
     7081 attggcgaat gtacagactg atgattggac taatggaatt gttggatgct tggatgatca
     7141 tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt
     7201 tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac
     7261 tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa
     7321 acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag
     7381 ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg
     7441 cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt
     7501 gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac
     7561 gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa
     7621 gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg
     7681 tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc
     7741 cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc
     7801 ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat
     7861 tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt
     7921 tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac
     7981 atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta
     8041 gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc
     8101 aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca
     8161 gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt
     8221 caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca
     8281 gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc
     8341 catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa
     8401 aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt
     8461 ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc
     8521 aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg
     8581 ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag
     8641 atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg
     8701 gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa
     8761 cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc
     8821 aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag
     8881 atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt
     8941 tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt
     9001 ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag
     9061 cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta
     9121 ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg
     9181 ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa
     9241 gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc
     9301 ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca
     9361 gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa
     9421 ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg
     9481 gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta
     9541 tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc
     9601 gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc
     9661 aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt
     9721 caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa
     9781 tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc
     9841 tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt
     9901 acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa
     9961 cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc
    10021 atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg
    10081 ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa
    10141 atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa
    10201 gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca
    10261 ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga
    10321 atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa
    10381 gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag
    10441 attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg actgcaagta
    10501 tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg
    10561 acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc
    10621 ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa
    10681 caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg
    10741 cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac
    10801 cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg
    10861 gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa
    10921 aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg
    10981 ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa
    11041 gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag
    11101 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
    11161 cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg
    11221 tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg
    11281 cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt
    11341 cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata acaattggat
    11401 tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa
    11461 caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc
    11521 gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga
    11581 gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc
    11641 ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt
    11701 gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg
    11761 agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc
    11821 aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc
    11881 gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag
    11941 atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc
    12001 cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc
    12061 tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc
    12121 gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc
    12181 ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg
    12241 tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat
    12301 tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc
    12361 tcggtttatc aaacttttaa gaacaatttt tattagttgg caagtagttt tggatagaga
    12421 aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta
    12481 tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa
    12541 tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag
    12601 aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc
    12661 cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt
    12721 ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa
    12781 atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa
    12841 aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc caatt
//
LOCUS       DACCZY010000084        12849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCZY010000084 DACCZY010000000
VERSION     DACCZY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.085"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            126..3653
                     /locus_tag="IVZ67_001991"
     CDS             126..3653
                     /locus_tag="IVZ67_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8166061.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IVZ67_001992"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IVZ67_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8166062.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IVZ67_001993"
     CDS             7441..7782
                     /locus_tag="IVZ67_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166063.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IVZ67_001994"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IVZ67_001994"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8166064.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IVZ67_001995"
     CDS             9139..11559
                     /locus_tag="IVZ67_001995"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8166065.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVZ67_001996"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVZ67_001996"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8166066.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4359 a         2239 c         2727 g         3524 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataac
//
LOCUS       DACCZY010000085        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCZY010000085 DACCZY010000000
VERSION     DACCZY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.086"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IVZ67_001997"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IVZ67_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8166067.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="IVZ67_001998"
     CDS             complement(324..893)
                     /locus_tag="IVZ67_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8166068.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="IVZ67_001999"
     CDS             1079..1981
                     /locus_tag="IVZ67_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8166069.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IVZ67_002000"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IVZ67_002000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8166070.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IVZ67_002001"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IVZ67_002001"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8166071.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IVZ67_002002"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IVZ67_002002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8166072.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="IVZ67_002003"
     CDS             8549..8734
                     /locus_tag="IVZ67_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8166073.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="IVZ67_002004"
     CDS             complement(8796..9992)
                     /locus_tag="IVZ67_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8166074.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="IVZ67_002005"
     CDS             complement(10162..11598)
                     /locus_tag="IVZ67_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8166075.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="IVZ67_002006"
     CDS             11891..12700
                     /locus_tag="IVZ67_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8166076.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DACCZY010000086        12349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCZY010000086 DACCZY010000000
VERSION     DACCZY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.087"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..472
                     /locus_tag="IVZ67_002007"
     CDS             <1..472
                     /locus_tag="IVZ67_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8166077.1"
                     /translation="KFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTL
                     FVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLL
                     PKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            481..708
                     /locus_tag="IVZ67_002008"
     CDS             481..708
                     /locus_tag="IVZ67_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166078.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1344..1715
                     /locus_tag="IVZ67_002009"
     CDS             1344..1715
                     /locus_tag="IVZ67_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166079.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1971..2213)
                     /locus_tag="IVZ67_002010"
     CDS             complement(1971..2213)
                     /locus_tag="IVZ67_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8166080.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2245..3147)
                     /locus_tag="IVZ67_002011"
     CDS             complement(2245..3147)
                     /locus_tag="IVZ67_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8166081.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3160..3348)
                     /locus_tag="IVZ67_002012"
     CDS             complement(3160..3348)
                     /locus_tag="IVZ67_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8166082.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3362..3925)
                     /gene="amaP"
                     /locus_tag="IVZ67_002013"
     CDS             complement(3362..3925)
                     /gene="amaP"
                     /locus_tag="IVZ67_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8166083.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3963..4856)
                     /locus_tag="IVZ67_002014"
     CDS             complement(3963..4856)
                     /locus_tag="IVZ67_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8166084.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4934..5872)
                     /locus_tag="IVZ67_002015"
     CDS             complement(4934..5872)
                     /locus_tag="IVZ67_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8166085.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5906..6256)
                     /locus_tag="IVZ67_002016"
     CDS             complement(5906..6256)
                     /locus_tag="IVZ67_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8166086.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6289..7191)
                     /locus_tag="IVZ67_002017"
     CDS             complement(6289..7191)
                     /locus_tag="IVZ67_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8166087.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7184..8041)
                     /locus_tag="IVZ67_002018"
     CDS             complement(7184..8041)
                     /locus_tag="IVZ67_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8166088.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8375..9184
                     /locus_tag="IVZ67_002019"
     CDS             8375..9184
                     /locus_tag="IVZ67_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8166089.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9224..9721
                     /locus_tag="IVZ67_002020"
     CDS             9224..9721
                     /locus_tag="IVZ67_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8166090.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10106..10179)
                     /locus_tag="IVZ67_002021"
     tRNA            complement(10106..10179)
                     /locus_tag="IVZ67_002021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10143..10145),aa:Ile,seq:cat)
     gene            10366..10689
                     /locus_tag="IVZ67_002022"
     CDS             10366..10689
                     /locus_tag="IVZ67_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166091.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10853..11107
                     /locus_tag="IVZ67_002023"
     CDS             10853..11107
                     /locus_tag="IVZ67_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166092.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11177..11422
                     /locus_tag="IVZ67_002024"
     CDS             11177..11422
                     /locus_tag="IVZ67_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166093.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11498..11863
                     /locus_tag="IVZ67_002025"
     CDS             11498..11863
                     /locus_tag="IVZ67_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166094.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3544 a         2310 c         2120 g         4375 t
ORIGIN      
        1 gaaatttaaa acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt
       61 gttcggcgta cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg
      121 gcagaccaca ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc
      181 ttatttctta ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag
      241 cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg
      301 cttacttcct aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt
      361 aggaatcgct gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa
      421 actgcttgaa catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac
      481 atggcaatta tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa
      541 ttgagtgaga aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca
      601 aaagccatcc gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt
      661 gatttgttga cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa
      721 caaacagtaa ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta
      781 cgtctattat tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg
      841 tactgactca tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa
      901 ctggtttcct gttacgttgt agttggatct gcttttagta taatacttta ttcattccac
      961 cagactgata gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt
     1021 cataccttga agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt
     1081 cttttttagt aagctcccat atatactgta aagccttcta tatattatgt catgttgctt
     1141 tttaacattt actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt
     1201 ttttattttt aacattattt tgataataat gattatttat ttaaaaatac attagataac
     1261 atttgttacg acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa
     1321 ctcacacaat taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg
     1381 tgatctatat tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg
     1441 atacgattgc tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta
     1501 cgagctatta ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct
     1561 ttttcaatct ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc
     1621 atttgtggat catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta
     1681 tctcaagaaa atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga
     1741 cacatttaag gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc
     1801 tgtgacaaaa gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat
     1861 cttcgataat atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct
     1921 tattacttga tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt
     1981 cgagcaaaga atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt
     2041 gccattcctg caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca
     2101 actaaacctg ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct
     2161 cctgcaattg ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt
     2221 tcctcctaat gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt
     2281 ttgtgcgccg cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc
     2341 ggtttttttc gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc
     2401 ttcttttgtt ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat
     2461 attttttact tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt
     2521 gccatattcc acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc
     2581 gatcccagcc gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc
     2641 accgtttgca gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac
     2701 aactttatct tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt
     2761 tccttggtta tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact
     2821 aagtttttct tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga
     2881 aggatcgatt gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg
     2941 tgcatgcttt tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac
     3001 tggatctgga tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga
     3061 ttggtttggt gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt
     3121 gttattattt ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc
     3181 taagattttc gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa
     3241 gatcaataag gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag
     3301 taccccaaag atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc
     3361 cctattctac tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt
     3421 tcaccgatac tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt
     3481 ctttcatcaa tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta
     3541 cattgacggc gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt
     3601 catcttcatg caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact
     3661 ccccatcacc atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa
     3721 gtaacgtgat aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca
     3781 cgtatgaatt agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg
     3841 ttgcacttaa tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc
     3901 cgatcgtttt tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact
     3961 tcttataagt tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt
     4021 gctttattcg atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt
     4081 tctagtaaac tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt
     4141 tgtaatggtg tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca
     4201 agctgttttg ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct
     4261 aataaatgtt cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct
     4321 gctgggatat gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata
     4381 aatgtgtctt tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt
     4441 cctgcgttta gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt
     4501 tctttaggtg cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca
     4561 tctttcacgt attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga
     4621 gcgatatctg ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct
     4681 ccaccagtga ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct
     4741 ccacaatcag gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta
     4801 ggaaatttct ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct
     4861 gtattcctcc tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt
     4921 ttaaatagat tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg
     4981 ctgctatcaa agagccaaag actacgagta taaagcctag agcagaactt ttacccggta
     5041 atcctcctgt attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg
     5101 cagctgaatc tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata
     5161 gatcaccaat actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct
     5221 gtctcaaatg gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag
     5281 aattccataa ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga
     5341 tgtctctttc aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg
     5401 ctttatccaa agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt
     5461 aaatatctaa taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct
     5521 ttgtcagcag ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat
     5581 cggaaagtac aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta
     5641 gattaatcac taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc
     5701 gagaaacttc ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt
     5761 cgattgcttg ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt
     5821 tttcatctgt ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc
     5881 tcctttttgg actttctcga aactattagc tgaagaagca gttctttaat ttgatattat
     5941 aatcgagtac tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca
     6001 tgttcaatcg tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg
     6061 gatgctcgat tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa
     6121 agctgccttt tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg
     6181 cgagtgcgat ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag
     6241 acattgtaat ttccattcct actccccctc ttcagttgtg gtcttctatt atttaacaac
     6301 tacatctgtt tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca
     6361 acggaaagcc gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc
     6421 cttgctccac ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc
     6481 ttcgtattca cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc
     6541 ttgtgcattc caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc
     6601 atcttggtca tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg
     6661 cgtaatattt tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata
     6721 acgcattgat ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc
     6781 aacagctgct acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa
     6841 agcgatcatg tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc
     6901 cggaatcgca atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt
     6961 aaagaattct ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac
     7021 tgagaagcgt aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg
     7081 aacaaattta gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg
     7141 tacgaatttt gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc
     7201 ctactttcgt tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat
     7261 ctttcaacgt attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat
     7321 ttccttcttt atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca
     7381 gcgaagtctg catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat
     7441 tcaaagcatc ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag
     7501 ttttttctgt aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat
     7561 tgaagtacaa gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat
     7621 cgataatagc gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt
     7681 ctagtactaa cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata
     7741 agtttggtaa agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt
     7801 cttcgtagat atctttttcc actgtcagga attggatact tgcattactt gaatatttat
     7861 ttaccgtttc ttccaatcct tctttactat attctgatag accatcgtca atcacataaa
     7921 attttatgcg tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt
     7981 aaggagcata attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca
     8041 tcaaaatcac tcctttttct aatctttttc taacctgttc tacatctaga atattcctca
     8101 tttggtttta aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc
     8161 acaaaacgca tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt
     8221 gaccaaaaac tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat
     8281 aatgacaaag caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga
     8341 aaatagatat gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga
     8401 cggtaccttg cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa
     8461 atttgatgaa aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa
     8521 agatattttt tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat
     8581 tgctgaaatg aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga
     8641 tacactgaga aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg
     8701 agaaacccaa gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta
     8761 ttatgaacat ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga
     8821 tccttctctt agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa
     8881 gctacaaaga gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt
     8941 agaaatcacg cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct
     9001 tggaatcgaa tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt
     9061 gaattacagc gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc
     9121 aaaccaagta acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta
     9181 ttaaaaagaa taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga
     9241 tatgcaaaat catattcttg atccaaacag cgaattttat attccagagt cagatcaatt
     9301 ggcagaacgt attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt
     9361 cacacgggat ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt
     9421 gatccctctt ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat
     9481 tccacctgaa actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa
     9541 agagatcgaa gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct
     9601 gcagagtgct tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa
     9661 acacggagaa tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata
     9721 aaaatatcta tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt
     9781 ttagtcctac gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac
     9841 ggcaggctca cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt
     9901 aacaaacggc aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa
     9961 acgctgattt cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa
    10021 gaacagccaa gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc
    10081 agggcagctt tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg
    10141 ttatgagccg tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt
    10201 tgttactttt caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa
    10261 ataatattgt tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt
    10321 tatttgctta ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt
    10381 attttgccat ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa
    10441 ttaaattatc aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc
    10501 cttttgcttc ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca
    10561 aaagtatttg ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg
    10621 aatgcgatcg tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt
    10681 tctcgataga taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct
    10741 gctttttcat ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg
    10801 tatgcaataa catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac
    10861 gttggtgaga gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct
    10921 tatgaattgg aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt
    10981 tatgtggaaa gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt
    11041 atcaggactg gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa
    11101 acattgagta ctattgattg catgtgtagc tttctatagt cattataact attctactat
    11161 aatgaaaggt gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg
    11221 tctgaatttc ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag
    11281 tattccttta ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga
    11341 attaatctat atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga
    11401 aacagcaagc aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt
    11461 ctaaggttaa caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt
    11521 gatttcaaag cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag
    11581 aggaaatggc tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta
    11641 tagattattc caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc
    11701 agcaccagag tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt
    11761 acctactgta gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga
    11821 atgtttagca aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct
    11881 tctatctcct cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt
    11941 aagttttagg gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt
    12001 catcgccaac ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat
    12061 cttcgacgtc tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt
    12121 ttctgttgct tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc
    12181 ctaatattcc catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt
    12241 ttaactcctc tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt
    12301 cagaacaacc tgccattgct agcgttaata ttgatagtaa aaatagttt
//
LOCUS       DACCZY010000087        12240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCZY010000087 DACCZY010000000
VERSION     DACCZY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.088"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(449..1174)
                     /locus_tag="IVZ67_002026"
     CDS             complement(449..1174)
                     /locus_tag="IVZ67_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166095.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1328..1654)
                     /locus_tag="IVZ67_002027"
     CDS             complement(1328..1654)
                     /locus_tag="IVZ67_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166096.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1691..2056)
                     /locus_tag="IVZ67_002028"
     CDS             complement(1691..2056)
                     /locus_tag="IVZ67_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8166097.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(2093..2308)
                     /locus_tag="IVZ67_002029"
     CDS             complement(2093..2308)
                     /locus_tag="IVZ67_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166098.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2437..2772
                     /locus_tag="IVZ67_002030"
     CDS             2437..2772
                     /locus_tag="IVZ67_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166099.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2848..4845)
                     /locus_tag="IVZ67_002031"
     CDS             complement(2848..4845)
                     /locus_tag="IVZ67_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166100.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4943..5218)
                     /locus_tag="IVZ67_002032"
     CDS             complement(4943..5218)
                     /locus_tag="IVZ67_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166101.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5450..6184
                     /locus_tag="IVZ67_002033"
     CDS             5450..6184
                     /locus_tag="IVZ67_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166102.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6223..7365
                     /locus_tag="IVZ67_002034"
     CDS             6223..7365
                     /locus_tag="IVZ67_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166103.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7993..8478)
                     /locus_tag="IVZ67_002035"
     CDS             complement(7993..8478)
                     /locus_tag="IVZ67_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP8166104.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8480..9244)
                     /locus_tag="IVZ67_002036"
                     /pseudo
     CDS             complement(8480..9244)
                     /locus_tag="IVZ67_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9414..10607)
                     /locus_tag="IVZ67_002037"
     CDS             complement(9414..10607)
                     /locus_tag="IVZ67_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166105.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10691..10894)
                     /locus_tag="IVZ67_002038"
     CDS             complement(10691..10894)
                     /locus_tag="IVZ67_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8166106.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            11151..11393
                     /locus_tag="IVZ67_002039"
     CDS             11151..11393
                     /locus_tag="IVZ67_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166107.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(11315..11548)
                     /locus_tag="IVZ67_002040"
     CDS             complement(11315..11548)
                     /locus_tag="IVZ67_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166108.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11555..11986)
                     /locus_tag="IVZ67_002041"
     CDS             complement(11555..11986)
                     /locus_tag="IVZ67_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8166109.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3770 a         2494 c         1967 g         4009 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
       61 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
      121 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
      181 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
      241 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
      301 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
      361 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
      421 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
      481 aatcagttac ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta
      541 accgaagtgg aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat
      601 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
      661 tgattccttt atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta
      721 caaatgaatt aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg
      781 tatatatggc ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat
      841 tagttagtaa agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc
      901 taacagcttt aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt
      961 ccataaaccc cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt
     1021 tcatttctga gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct
     1081 gattcgtttt taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg
     1141 ccaaacaaat ccataataat tttttctttt tcatttttcc tcctatacac tgctgttata
     1201 acagtgacta atgtaaagat tttcccagca atcatcagac tattatttac agggaatcaa
     1261 aaaaactcct tagtaagctt acacctttag gagttcctgt ttgaacggta tgcataatac
     1321 atttaaatca atttaacatt aacacctcag tatatggtat ctctacatat acacggtctt
     1381 tttttggata gtactcgaca tttatattga tagcacttcc attgtctaac gtagctttag
     1441 cccggtacgt gttatcacca ttatcatgag tgatcgtcac atcatcacat gtccgattta
     1501 agttatactg ttcttgtaaa atctgagtta ccaatggtac agatgacttc tctagtccag
     1561 atgaacccgt cattcttgaa aaaattatac ctataatagc caatacaacg atacctataa
     1621 tgacaaactt cttattcata tttactttat tcaacttttt atctcctatc tccaaaatga
     1681 ttttctctca tcagtaactc attgacatcc ccaatagtac accaaagatg gctccagcta
     1741 cccctgctat catcaaaatc gtacccattt gttcatcttt ctctcgatat aagtaaccca
     1801 taataacagc aacagcacca aataccacag gaataaatgc taatgatact accatagaaa
     1861 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
     1921 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
     1981 tttcttgtaa tgctccgcaa aactggcaat acttactttc atttgttatt tctttaccac
     2041 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agctaactgt
     2101 ttactttatt aaatacatca gaaaacagct catttattgg tacacttaat atctcagaaa
     2161 gcattttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
     2221 gctgtgtaac attcattttt ttggctaact cagtttggga aagtcctttt tcttttctgt
     2281 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     2341 atatgatgta tttcaactca taaaatatat aaatacttca aatgtcatta caaatacaac
     2401 aacttgttgt actctactga gaaggggggt gtattaatga aacagaagca atatgcaaag
     2461 gtcattagag aaaagcgaaa agcactcggg tggacacaaa aagaattagc taaaaaaatc
     2521 ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagaacc taacctcgat
     2581 agcttaacag ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt
     2641 gacatagatt atgaggaaga gtttttggct ctctatcgct ctcttagtac tgaagatgca
     2701 attcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta
     2761 ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa
     2821 taagattaag agctatggat aacctgtcta gtttaatata attgggtctc ttctattcct
     2881 tactctccgt ccatcatctc ttgttacact tgtaaattga taaataggaa ctaattcaac
     2941 tcctgataat atcaattctt ttattacttc atctatccct gcccctttcg ctagatatac
     3001 taaaaaacta ctgcttgctt tcacattgta tagtttctta atatccttag aagctcgttc
     3061 aagagtgaat ccttgtttaa caaagtagcc tctgttttct attaaagcat ttttcacgct
     3121 gttatccctg acatttcgta ggcgttcaca aacagtgtta ataagttctt tttctgtcat
     3181 tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa
     3241 ttgtttttcc ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt
     3301 ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt
     3361 ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac
     3421 gtctaatcga tagacattgc ttctcttatg tttaaagttt cctaacgctt tctcttcctt
     3481 taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat
     3541 caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt
     3601 accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact
     3661 ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat
     3721 ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc
     3781 aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt
     3841 ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac
     3901 tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc
     3961 agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa
     4021 gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc
     4081 attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc
     4141 tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct
     4201 ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt
     4261 aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa
     4321 aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta
     4381 ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat
     4441 cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt
     4501 atgaactaaa ttgatagata aaaccttttc tatgcttaag tatctactat catctttact
     4561 caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac
     4621 tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa
     4681 tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc
     4741 taaacaccta tctcttacat cagttttttc tttttttgta actggcttct ctaataattt
     4801 ttctttatct acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt
     4861 ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt tattactata
     4921 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
     4981 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
     5041 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
     5101 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
     5161 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
     5221 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
     5281 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
     5341 atcgacatac acataatacc tcgataaaaa aggggtgtca atagttttgt aaaatttatt
     5401 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
     5461 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
     5521 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
     5581 tgacaaaaag actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
     5641 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
     5701 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
     5761 tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt
     5821 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
     5881 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
     5941 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
     6001 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
     6061 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
     6121 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
     6181 ctagtggtaa gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag
     6241 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
     6301 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
     6361 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
     6421 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
     6481 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
     6541 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
     6601 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
     6661 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
     6721 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
     6781 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
     6841 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
     6901 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
     6961 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
     7021 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
     7081 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
     7141 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
     7201 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
     7261 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
     7321 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
     7381 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
     7441 tctagctcct ataacaggtt tacccaacac tgccttccaa tgactctttt gggaatcaga
     7501 ggaaagaatc aggctttata gagatatttc atctaacaaa ttactttaga ctgacagtat
     7561 atatcagcag gtactgtcat cgggaacgta gccaatatcg tatccaaaac agcaggtaga
     7621 gaagctattt gaggattcaa gttattctat ttcaaccatg aagttaagtt cattaaaaat
     7681 aaagatagtt ggtatttcaa taatttttgt atagagttaa tcactttatt gatgctaatt
     7741 catcttgatt ggtattataa tcgtattcaa gttgcgtggc gtgcttttgc tagaaaatat
     7801 ataactgttc tttataaaga aatataagga ttaaacattt tttatcatag agttttactt
     7861 atctatagaa ttatgtctgt tcctaattca gtttgatgaa ttaactgata aaaaaatatc
     7921 tactatttaa agatacaggg ataagtaagt aaaaaaataa catgtcatta gatagtagac
     7981 agttccaact ctttatttac cttttttgta tgctgcactg tcaatccaag ttcctaaatt
     8041 gttgtatcca tcatcactta tataatcata ttggccatca ataatttcgt caaaccacaa
     8101 atattctcca tctggttttt gcccaattat tttaattact gattgactag tagttgtcat
     8161 ctggttaaaa tcttttattt tacctatatt aacggccaaa atagcatttc ctcgcttata
     8221 actctcctgg atttcatatt taacatattc tcgatcaaga gtttctttac ctatcagcac
     8281 cacagtcact gatgttccaa ttaattgatt atctatccag ttttttatag caatatctcc
     8341 attacgttta actttctcaa attcagcctt atctacaaaa ccagcttcct ttgttcctcg
     8401 caaagtccag ctgtttctaa ctaccattgc tcttgttaca tctgcataat ggaaactaaa
     8461 aaaaaccctt ctcaccatat taatcctcct gaatactatt tattaaactg acaatttgtt
     8521 caactttttt atctacttct ccttcagtat ctaatttttc tagcgcttga aatttacttt
     8581 ttaaggaatc atcaaaataa tcactgaaat tctcttgaat cttatcaaac aaaaccttac
     8641 ttgcaccttt ggtaaatcca atcggaacta ctatattttc attttgaaca gataattcaa
     8701 attcttcaat cattccatct gctgtaacta tttcacctga ttcaagtttg tttccaaaaa
     8761 caaaaatact aatcccagac tctgcaataa tgttttctcg atatcttctc cactcatgag
     8821 gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg tatgttattt tttcttaaat
     8881 ttcttctctg ctttagaact cctgaaacaa tactattacc aattccaagt ccgtaacctg
     8941 atactattct ataaccggat tcagaaagcc tatatgctaa tttctcgaca aattttaatc
     9001 ctttcgattc agatgtaaat ggagtataat caacagcact tcccgaaata aaaatagttt
     9061 ttctcaaata cttttttcta atacttttca aaatatcagt aatctcagaa taatcatcta
     9121 cttcaacaac gtttatcgaa aatctggaca aatcattgat ttgtagtctt ctttttgtca
     9181 gttcataatt atatctctct tgatcaaaaa aatcctcaga atcgacaaaa ttttccttta
     9241 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     9301 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     9361 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     9421 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     9481 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     9541 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     9601 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     9661 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     9721 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     9781 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     9841 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     9901 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     9961 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
    10021 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
    10081 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
    10141 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
    10201 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
    10261 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
    10321 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
    10381 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
    10441 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
    10501 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
    10561 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
    10621 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
    10681 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
    10741 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
    10801 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
    10861 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
    10921 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
    10981 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
    11041 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
    11101 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
    11161 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
    11221 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
    11281 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
    11341 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
    11401 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
    11461 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
    11521 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
    11581 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
    11641 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
    11701 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
    11761 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
    11821 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
    11881 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
    11941 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
    12001 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
    12061 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
    12121 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
    12181 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DACCZY010000088        12063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCZY010000088 DACCZY010000000
VERSION     DACCZY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.089"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..573)
                     /gene="frr"
                     /locus_tag="IVZ67_002042"
     CDS             complement(16..573)
                     /gene="frr"
                     /locus_tag="IVZ67_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8166110.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(576..1298)
                     /locus_tag="IVZ67_002043"
     CDS             complement(576..1298)
                     /locus_tag="IVZ67_002043"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8166111.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1434..2315)
                     /locus_tag="IVZ67_002044"
     CDS             complement(1434..2315)
                     /locus_tag="IVZ67_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8166112.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2414..3196)
                     /gene="rpsB"
                     /locus_tag="IVZ67_002045"
     CDS             complement(2414..3196)
                     /gene="rpsB"
                     /locus_tag="IVZ67_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8166113.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3555..4034
                     /locus_tag="IVZ67_002046"
     CDS             3555..4034
                     /locus_tag="IVZ67_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8166114.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4251..5342
                     /gene="phnW"
                     /locus_tag="IVZ67_002047"
     CDS             4251..5342
                     /gene="phnW"
                     /locus_tag="IVZ67_002047"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8166115.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5335..5463
                     /locus_tag="IVZ67_002048"
     CDS             5335..5463
                     /locus_tag="IVZ67_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8166116.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5467..6012
                     /locus_tag="IVZ67_002049"
     CDS             5467..6012
                     /locus_tag="IVZ67_002049"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8166117.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6466..8157)
                     /locus_tag="IVZ67_002050"
     CDS             complement(6466..8157)
                     /locus_tag="IVZ67_002050"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8166118.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8577..9524)
                     /gene="arcC"
                     /locus_tag="IVZ67_002051"
     CDS             complement(8577..9524)
                     /gene="arcC"
                     /locus_tag="IVZ67_002051"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8166119.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9639..10658)
                     /gene="argF"
                     /locus_tag="IVZ67_002052"
     CDS             complement(9639..10658)
                     /gene="argF"
                     /locus_tag="IVZ67_002052"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8166120.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10749..11978)
                     /gene="arcA"
                     /locus_tag="IVZ67_002053"
     CDS             complement(10749..11978)
                     /gene="arcA"
                     /locus_tag="IVZ67_002053"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8166121.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3288 a         2509 c         2189 g         4077 t
ORIGIN      
        1 gtttttatga ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc
       61 tacttctttg atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc
      121 atcttcggta atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg
      181 gatgttacga atcgcgattt ttgcattttc tgcttctttt ttaacatctt tagctaattc
      241 ttttctgcgt tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt
      301 tggactgatt ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt
      361 gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt
      421 caatggtgtt ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc
      481 acggcctgcg cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt
      541 ttcttttgct tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg
      601 atattttcgc ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc
      661 agtggaatat cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct
      721 ttggcaataa cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc
      781 ggatcagcag aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg
      841 atttctgccg cgcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg
      901 cctgcaaaga taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat
      961 ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt
     1021 tccaacgtat cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca
     1081 gcttgagcac gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgccg
     1141 cccacaacga ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt
     1201 tctttgatta ctggtggttt gattccaaat cctgcgtccc cagctaaagc ttctccactc
     1261 aattttaaga ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct
     1321 attgccattt taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg
     1381 cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt
     1441 ttcacttggc tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca
     1501 acttcgaaac gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca
     1561 gtcatatcag gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc
     1621 ttcaagcgtc cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct
     1681 tgttctgtaa gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt
     1741 acataacgag gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca
     1801 gttgttccat ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca
     1861 aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg
     1921 ataacttgag ttgcttcgat caagtcagat tcaattgtac ctttatctgt tttgattttc
     1981 attgcagctt ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact
     2041 aaatcttgga acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt
     2101 gctgcagtgt tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt
     2161 ttcttcgcag cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg
     2221 ccttctactt caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt
     2281 agttctttta ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc
     2341 tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct
     2401 ccatcattca aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt
     2461 cgattgatgt tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt
     2521 ggtttccttc gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg
     2581 catcgtcgtt tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca
     2641 tagctacgat tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg
     2701 ggtcaacgat gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt
     2761 tttcaagacg ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa
     2821 aagttccatc ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag
     2881 tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac
     2941 gagttgcttc ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta
     3001 cgccgccttc ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg
     3061 ttttttgcaa gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt
     3121 ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca
     3181 tagaaattac tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt
     3241 ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg
     3301 ctgatgcata aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa
     3361 tatacttttc tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa
     3421 cagtagattt ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa
     3481 agacgaatat tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt
     3541 aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag
     3601 aattcccgat ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg
     3661 ccacgcaggc aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg
     3721 aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg
     3781 aggaagagga tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc
     3841 actttttgac gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag
     3901 atgagatcaa acctccctct attgtcagta caattgctgg attcgatacg atcatggtca
     3961 tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag
     4021 tatcactttt ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca
     4081 atattttcct tattgaataa acctttacac tctttacaaa aatacatcat tttttattca
     4141 aatttttcaa caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga
     4201 gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat
     4261 tattaacttc tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc
     4321 attgtacttg ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg
     4381 atcttgctca agtatcggaa ggaatataca caacggtctt aatgcaaggt agcagctcct
     4441 tcggggtaga atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt
     4501 caaacggcgc atatagacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata
     4561 ccgtttatga ggtatcggaa catcaaatcc tccaagctca tgtgattaca cagctgattg
     4621 aagagaatcc gtctatctcc gcactagcaa tggtccatag cgagactact tccggtattt
     4681 tgaataattt ggaagagatt gctgctgtca cacaagaaag agatcttttg tttatagtgg
     4741 acgcgatgtc aagctttggt gggataccga tttgtgtagc cgaactaggg attgattttt
     4801 tgatttccag ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta
     4861 ataaagcgga actggaaaaa cgaaaaaatg ttagccgaag tttgtcactt gatctatatg
     4921 accagtaccg cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata
     4981 cggttttggc ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc
     5041 gatattcacg ttatctcgcc aataatcatg tgctacgaca aaaaatgaca gagatggggt
     5101 tccagccttt cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg
     5161 actggcgatt tgattttaat catttttatc agtatttaaa gcaaaatgga tttgtgatct
     5221 atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc
     5281 cggaagatat tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt
     5341 aaaactgtaa tttttgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc
     5401 cacgctttta gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa agaaatccgc
     5461 taaccgatgg gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc
     5521 aaaaaacaat ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg
     5581 tatcaacaat ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc
     5641 gacacattgg aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgaat
     5701 ggatttaatg ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga aaggtattca
     5761 ccagacttca tggcaacttt tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg
     5821 atccataaaa acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga
     5881 gatacggtct cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt
     5941 gaaggtagtt cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa
     6001 aagatcgttt aaaacagtct gttgctctta gattccaaca agccggcgct gatatttgtg
     6061 taaacaattt aaaagagtta acagaataca ttttgtacgc tgacatgaat ccagaatgac
     6121 ttttatacaa aataagaggt tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca
     6181 agcaaaaata gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt
     6241 ttgaacaccg tttattcgaa aaaaaaaagg gagctgtgac acgacttttg tcacagtccc
     6301 cttatttgca aacaaacagt gtttaagaga caacctctcc actaaacggt gttcattcag
     6361 ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt
     6421 tctctaatac tcggatcaga acgctgattt cacattctct ttttcttaca tcttatctgg
     6481 tgcatgaagt cctagcaaac gaaggtcttc tttcaataat acagtcactg cataaactag
     6541 agctaagcgt gcttcttttt gttcatcatc agctaaaatc ttcgtatgag catagtattt
     6601 gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc actgacggtt cgtatttttc
     6661 cccagcagac aagaccgttt cagggtattt ttgaactaat ttaaccactt cccagctggt
     6721 atcgtcattc aatgcatagt ctgctgtttc tgaagggaca aatcctgctt tttccaacag
     6781 gctgactgca cgagcatgtg tatattgcac ataaggtcca gtttctcctt caaatcgaac
     6841 aacttcttcc aggttaaagt caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat
     6901 gactgcccca accccaactt gtttagctac agcatctttg ttttccaaat cagggttttt
     6961 ctcgctgatt tgttctttag cagattggat tgcttcattt aatacttctt ctaataagac
     7021 gatctttcct ttacgagttg ataatttttt accaccttgc gtgatcaaac caaatggaat
     7081 atggtgcatg tcgtcagacc agtcaaagcc catttccttc aagacagctt tcaactgttt
     7141 aaaatgatag ctttgttcat tccctactac atatagagat tgtttgaagt cgtaagtacg
     7201 tttacggtac aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt
     7261 cttaatcaac gctggattca ggtcataagc agataggtca acgatctctg cacctttatc
     7321 ctcgttcagc aaatgttttt cttctaaaag tttgacgatt tcatccatct tgtcattgta
     7381 gaaagcttca ccgttcaacg aatcgaaacg tacttccagc agatcataga ttttattgaa
     7441 ttctttcatc gattcatcac ggaaccattg ccataattga atagcctctt tatcgccttc
     7501 ttccagacgt ttgaaccaag cacgagcttc ttcatttaat tctggttctg tttcagctac
     7561 ttcatggaat tggacgtata aacgaagcaa ttcattgatt ggttctgctt taacagcttc
     7621 ttctgtaccc cattttttgt aagcgacgat cagttttcca aactgtgttc cccagtcacc
     7681 taaatggttg atgcggattg gctgataacc gattttttcc ataatgaaac cgatcgaatt
     7741 accgataact gtcgaacgca aatgtcccat tgagattggt ttggcaatat taggagaaga
     7801 catgtcgatt ggtactgttc cttgattccc gatgttgcta tccccataat gttctttttc
     7861 tttcactact gtttgcaaaa cttttttgct gataagttct ttattcatga aaaagttcaa
     7921 gtaaggtccg acaacttcga ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc
     7981 tgcagcgatt tgctgtggcg cttttcggta aacttttgct aatgaaaagg ctggaaatgc
     8041 aacatctccg tgttcagctg attttggatt ttctagtaat tgctcgactt gttccagtgt
     8101 taaatcatct ttgacagcgt catagacagc ttttgcgaca agatctttat tattcatctt
     8161 tattcctcct aaagtgttcc cttgattgtt aaacaaaaaa aatctctagc gacggcatac
     8221 ttagtatgcc atcactagag acgagatttc ccgcggtacc actccaattg tcctaaaaaa
     8281 ggaccagctc catctgatat cggtagacga ccgtctgttt aaaacagaat tctccaagtg
     8341 cgcttcgttg tatcttcttc attcggcttc caccacttcc gaattcgcta gccaagaatg
     8401 atacaagtac tctcttgttc aacaaatctt agtcttatta aactttcttg aattatatca
     8461 gaaaaaaaaa cgaaaggcta gtcgtttcat aaaaaaacaa tccagatggc ctaaaggaga
     8521 agaaatacca cctggatagc tataaatcat ttcgtaaata cgaaagactt aacaaattaa
     8581 tcagctacga taatcgttcc ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa
     8641 gtgatgattg ccttagcgtt tggttttgct tcaacaaatg caatagctgc ttctactttc
     8701 ggaagcatgc ttcctggagc gaattgcttt tcatcaatgt attgtttcat ttctgaaaca
     8761 gtaacagttt ctaatttctt ttgatctggt ttttggtaat tgacatacac attgtctact
     8821 cctgtcaata cgatcagcaa atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa
     8881 tctttatcga ttacggcttc tcgtccttct aatccagtag ctgtttctac aacaggaatc
     8941 ccaccgccac caacagagac tgtgataact ccttgatcga ccagcgtttc gatgacacgt
     9001 gcttctttaa tcgagatggg tttaggggaa gcgactactt ttctccatcc acgtccagca
     9061 tcttctttga atgtcacagt ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg
     9121 tagaatggac cgactggttt acttggattt ttgaatgaag gatcattttc atccacaata
     9181 acttgagtga ctaaagaaac cacatcttta tcgatccctt tttccttcag tacttcgccc
     9241 atggcgtttt gtaaccagta accgatagat ccttctgtca ttgcaacaca tgtatctaaa
     9301 ggcattgccg gtgttttctc agaatcagca gcttgctgct gaatcaatag gttaccgact
     9361 tgcggaccat ttccgtggga gatgatcaat tcatctcctt gttcgataaa tttcactaaa
     9421 tactttgctg tttccattaa cgcttcttgt tgggcttttg cacttgcatc cgtagacaag
     9481 atggcattgc cccctaaagc gacaactact tttcgtttgg ccatttcatt cgtcctttct
     9541 tcagttctac cgaattttct agataacaaa acgagccagc gttttgtgat tttccctcaa
     9601 tgctgactcg ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg gataaacaag
     9661 ttacctaatg ttgctgccat gatcgctttg attgagtgca tacgattttc tgcttggtca
     9721 aattgacgag catatttgct gcggaagact tcatctgtta cttccatttc agtgatgcca
     9781 aagcgttctt tcatttgttc accgtaaaca gtgtttgtat catggaaagc tggcaagcag
     9841 tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta ccatttccat attgatttgg
     9901 taaggtttca acaatttgat acgttcttcg aatttatctt cttcacccat tgatacccat
     9961 acatcagaat aaaggacgtc tgctccgtct acacctttat caacatcgtc ggtaatcatc
    10021 aattgtgcac cggatttttc agcaaagcct tctgccattt tgacgatttc ttcttcaggt
    10081 tgtaattctt ttggtgcaac gatacgcata ttcgctccta aaattgcacc tgtgactaat
    10141 aaagagttag ccatattgtt tcgaccatct ccgcagtaag caacagtgat accttctact
    10201 gttccaaagt tttcttggat agttaaaaag tcagcgatca tttgtgttgg atgccattca
    10261 tcagtcaagc cattccaaac tggaacacca gagaattctg ctagttcctc taccattttt
    10321 tgactgaatc cacggaattc gattccgtca aacatacgtc ctagtacttt agctgtatct
    10381 tctgtggatt cttttttacc taattggata tcatttgcac ctagatattc aggatgtgca
    10441 cctagatcga tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt tttttcaaat
    10501 aaaagcgcaa tattttttcc ttctagatag tgatgtggga taccacgttt tttcaagtct
    10561 ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa gtctttttct
    10621 gctaataagc ttcttccttg gaatactgat tctttcatat taaacattct cctttagatt
    10681 gtttttattt aagattaaag aggaaatccc gacggcagct gacagtaaga ctgccgtcag
    10741 gaaacaaatt acttcaagtc ttcacggaca agtggttggc tcatgcagcg tggaccacca
    10801 cgacctcgtg acaattcact tgaattgatt tctaacactt tgatgccata gctacgaagc
    10861 aattcgttcg atacataatt tcggttataa gtgactacaa cacctggagc gattgccaat
    10921 gtgtttgatc catcattcca ttgttctcga ggtgcaacga tctcatctcc attaccagtt
    10981 gggatcagta caagatcatc taatccaaga gcagctttca tcgtttcgtg taagttatct
    11041 gaatgagtaa tcttgatatc atcaccatct ggttcgattg taaagacatc catcttaccg
    11101 tctttacttt gaattgctgg atggatagtg aatttatcgt gatctaccat tgtaaatacg
    11161 gtatccaaat gcatcattgc acggttgttc ggaattttga ttgccaacac tttttcaaat
    11221 cctgaatttt ttgcgaatag gtggcgtgct aatttttcta aagctttcgc attagttcgt
    11281 tgagaaatcc caacagctac tactttatcg cttaagatca attcgtctcc gccttcgata
    11341 tgatctggat tttcacgatc caaccatact tcgattcctt tatttgcaaa tcttggatga
    11401 tgtttcaaaa tatattctgt aaacatcgat tcacgacgac gtgcttcaaa agtcatctta
    11461 ttgactgtca tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta caagtttggc
    11521 attgggtcca tgtaaaatgg atagtcatcg tctgctgaca gatcatatag actgctagaa
    11581 cgtacttcga tatcttttgt acgaacccca gccatgattt tatctaccat ttcttgcgtt
    11641 tccattgata gaaggaactc atgaagaccc tctcttacag cattcgatgc aatatgagat
    11701 tcatccaaca ttttattcaa gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc
    11761 gctaattttt ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg
    11821 tcatgttctt cttgagcaat cggaagatat ggaatatcat cgaacaacag acgatccata
    11881 atatctggtg tcaaattttc cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat
    11941 ttccctattt cagagaaaac gtgaataggt ttatccatgt tgtgttgcct ccttcttttt
    12001 gattacatgt ataggatatc ctccttttgt aatcactttc ataattttgt ctcattcaaa
    12061 atg
//
LOCUS       DACCZY010000089        12049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCZY010000089 DACCZY010000000
VERSION     DACCZY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.090"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..204)
                     /locus_tag="IVZ67_002054"
                     /pseudo
     CDS             complement(1..204)
                     /locus_tag="IVZ67_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(458..2093)
                     /locus_tag="IVZ67_002055"
                     /pseudo
     CDS             complement(458..2093)
                     /locus_tag="IVZ67_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2532..3401)
                     /locus_tag="IVZ67_002056"
     CDS             complement(2532..3401)
                     /locus_tag="IVZ67_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8166122.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3421..4542)
                     /locus_tag="IVZ67_002057"
     CDS             complement(3421..4542)
                     /locus_tag="IVZ67_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8166123.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4544..4939)
                     /locus_tag="IVZ67_002058"
     CDS             complement(4544..4939)
                     /locus_tag="IVZ67_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8166124.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4958..5755)
                     /locus_tag="IVZ67_002059"
     CDS             complement(4958..5755)
                     /locus_tag="IVZ67_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8166125.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5757..6545)
                     /locus_tag="IVZ67_002060"
     CDS             complement(5757..6545)
                     /locus_tag="IVZ67_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166126.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6559..7035)
                     /locus_tag="IVZ67_002061"
     CDS             complement(6559..7035)
                     /locus_tag="IVZ67_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166127.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7107..9764)
                     /locus_tag="IVZ67_002062"
     CDS             complement(7107..9764)
                     /locus_tag="IVZ67_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8166128.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9783..10187)
                     /locus_tag="IVZ67_002063"
     CDS             complement(9783..10187)
                     /locus_tag="IVZ67_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8166129.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(10419..10676)
                     /locus_tag="IVZ67_002064"
     CDS             complement(10419..10676)
                     /locus_tag="IVZ67_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8166130.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(10801..11133)
                     /locus_tag="IVZ67_002065"
     CDS             complement(10801..11133)
                     /locus_tag="IVZ67_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166131.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            complement(11666..>12049)
                     /locus_tag="IVZ67_002066"
     CDS             complement(11666..>12049)
                     /locus_tag="IVZ67_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8166132.1"
                     /translation="AFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHK
                     GCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPV
                     GYRNQKLAQRILDNELGCANASEAV"
BASE COUNT         3841 a         2370 c         1741 g         4097 t
ORIGIN      
        1 atatccttcg taatccttac tgttgataat tgctttcata ttaggaattg gatcttcacc
       61 tggcatatat tgcagattag aagcatgtaa ttttttctca gaaacaaacc actcattgga
      121 aaagacatga atcaaatcat cacttacttg tgtaaaatag cgacgtttaa tgacaaagat
      181 atcttcattg ctatctagtg taccttcatt tctttgtgct taaattcaaa gataattttc
      241 tcaataaaga tttttgattc cagtaagatt cctttactcc ttttacaaca tagcgttgtc
      301 ctttgttttg aaacatcttc ttcccctctt tgccattaaa aatagcccaa ttctctataa
      361 agaagattaa gcttcattat tataatataa agtttaaatt tggtttatta cttacttttt
      421 cttattctct agtaagctaa tattgaaaaa aatgtaatca gatcattatt tttatatgtt
      481 gtaacttcct gataagaaaa ctaattaaaa gaaagaggta actacataaa ctaattaaaa
      541 aagctaactt catagttgga ggataaatgg aagaaacttc aatgattgtt tctccttgtg
      601 ttccttcaat cgacactagt cctattatca atctctcttt tttgtccatt tacttttgcg
      661 actgttcccc aataatcaaa tacaggaagc gttatttttt gtcctttaga ttcatgtact
      721 gtatagacta ttttcgaatc catcacttga taaggcatag tatgccattc cttgccaatt
      781 cttacttcat gattctgcaa atgttctttg tattgtaatg cttccgtggg tgcatagtca
      841 taaagtcctc cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt cattaccaag
      901 tgatctctct gagtgacaac tttatgttca tagtttgctt ttgttgcagc agatacagcc
      961 aacagtaata aaacaattgc cgttatatta actacttttc tttgcttagc tgatgtgctc
     1021 ttcatgaaaa cgaacgataa aatcaacgag ccaaacagta cttgaaagcc taagattcgc
     1081 caaggaaatt gaatcatttg aatgggtgta ttttgtaata aagaccatgg aaaaaatttg
     1141 gtggataaga gccaagagac taccgcacta aggtaaatca ctttttcttt gcgattaaat
     1201 cctttaaaaa agaagagtgg acagatcaaa gaaataatac caatgattcc taccgtaaag
     1261 gttgtcaggt cattattcat cgcaccaatc aaactatccg agacatttaa tgcttggtgt
     1321 tgtaaggtcg ttttatctgg ctcattaatc gattgaacca tgatttgtcg gaacatcgga
     1381 taccaaaagt atgctgttaa aagaatcgtc attcctactg cctttgacaa ctccacaatc
     1441 aaccggatgc tttctttttt tcttgtttca cttactacaa agccgcctaa gactagtaag
     1501 ccaacggcta ttacactcat aaaagtggaa agaacatgtg tataaatgag taagctcata
     1561 ctgactgcta atggaattgc acatggcttt ttctcataaa tcagtgcgta agcataatac
     1621 ataactatcg gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg atgataaata
     1681 tcaatcgtac ggtacccata aaatgtatat aaacagctaa atataaaagc cgcatcgatt
     1741 ctttttaaaa attttagtcc ataatgaaac atcagcatga tagtacaaac attcaatagc
     1801 caaacatata agatataact aacgattaaa ttattactta tccaataaaa aatgactgcc
     1861 ggaaaaaagg tcaagtaggg ataaaaataa ttcactccgc tcccataatg attaaacgtc
     1921 gtaaaattga ttggaccaga aaaaattgaa gataaccctt tgactctagc aaaatggaag
     1981 gcaatatccc ccgatgtaga aatagtatga acatttctaa aataattgat tgaataaagt
     2041 ccagcaataa ttgctaggag aatggacaat attatttttt tctttacttg cattacttaa
     2101 ttatatactc ctttcatgga ttgctttatc tagtataact taaattttaa ataaatcgtt
     2161 atttattttt actctcactt aaaaaagcac taaaattata agagagggag gtaatggtct
     2221 gaacaccaag aattgttcca acatcgaatt gcttaactaa tgagttcaaa gcttaattat
     2281 gtggctctta tatttaccga gcgtggattg cgatctcttt agcgaaacaa acatctttca
     2341 taaaaaaaac agccatatcc gctttcttgg atatgactat ttactactac tttactatct
     2401 ttcattcttc ttgtctcact tatgataagg cgctgggtat catgagtgaa tgatattgct
     2461 gaatacaaat agtccgacca cattaagttg atcggacttc ataagcattt tattgactaa
     2521 tacttttttg cttaagaatt gattctaaca tcttttgcta aagttaagca tcctatagtt
     2581 cctgcattat ctgctaactt aggtgtgaca atgtattctt cgatatttgg cgtatcgaca
     2641 tagccgttaa ttagttctaa aaaacgtctt tgtactttct ttttcagatg ttcttgttgc
     2701 atgacaccac caccaaaaat tataatatct ggtgaaagca tcagggtcgt attacaggca
     2761 cattgtgcaa tataatccgc ctcgatttcc cagaacggat cttctataga aagatcttgt
     2821 cccttggttc ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc gagacaatca
     2881 tgatgaaaag gacaagtacc agagaaagta tctcctgaat gtctcttaac taacgtatgc
     2941 cccatttctg gatggctaaa cccttcaaca aaaatcccat cttgtatagc tcctccacca
     3001 attccagttc caacagtgta ataaacaaca ctggataaac cttttccatg acctgaaaca
     3061 tactccccgt aacaagcagc attcacatcg gttgtccagg caattggaat ttcgaaatgc
     3121 ttattcaacg ttccaacaaa atcaaaattt ttccaggcag tttttggagt agaggtgata
     3181 tgtccatagg tctttgattc acgatggata tcaatcggtc caaatgaccc tactccaatg
     3241 ctttctaatt gttctttata gtgattaaag aaatcaatta ccaaactcat tgtttcattc
     3301 ggtgtagttg taggaaaact cactctttct aaaaccttta aatcatcatt gccaatggca
     3361 caaacaaatt tagtcccacc tgcttctatt gagccataca tttcttactc caccgtcctt
     3421 ttatctagga attcgttctt ccctgtctaa tagtttttcg aaaaactcat gtggttcctt
     3481 ttggtaccaa taggcaacag aagaaatatc gtcttgacgc tcataaagct tgatgtcatc
     3541 atttcctagg gtttggaaag taacacgtaa attttctttg aaactaatgg ggtcgggtaa
     3601 atgccagcgg tacaacccat gcatcggtaa agcatcatcg ccaaaaccat gaactggatt
     3661 tggattaggt tttccagcac taaaataatc ccgggtgtga tctcttttag attgaaaagg
     3721 atagcccata aatagcgtat taaaattttg agcatatgcc ctttctccaa agttcttttt
     3781 atggaaagcc caagcgccgc caaaataatc ttctgctcct gtagatgtta ttgtagggaa
     3841 atcttcatct ccatctatat aaaacttaaa ttctccttcc ccccaccagt atctttccaa
     3901 tgcgcacaaa gcaaagtagg ttcctacata atacccagtg cctttgattc catcaataag
     3961 ggtgtaatct tttccttttg tcgtgatggg ttcacggttc catttagcat gaaaatacat
     4021 cgcatcatcc ggtacttcag tcaatgcata attaaatgta taaaaaatgt gcttaataaa
     4081 ctcaggatgt tcattcgtta aagtaatctt agcttttttt cgaaatggca tctcaaaata
     4141 gcaattcatc cctgatgttg gattcactac gattggtaag gaattcactt cgcatcttgt
     4201 cccaaaccca ttacaaaaaa aatctcctaa tggactttct acagaaggag tggattcatc
     4261 atcccaataa atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc
     4321 tgtcactgtc atccaaaaat gacgtaaaat tccagggcct tccatttctg ctaaagtaat
     4381 tgtctctcca gcatttatcg gaacagaagg tgaccctttt ctcccaattc ctagatgact
     4441 cgctgtcatt gccgctttcc ctttttcacc atttttattt tctggtgaaa tagttctact
     4501 ttcctctccc ttgtggagcc atatattatt taaacaattc attttattcg tcctctatcc
     4561 tttcttcact tttttgatta ttcatataga tgatttgttg ttgtgcctcc gcaataattg
     4621 ctccaatatc taaattatca tttcctaact gcaaagcgat tgacattgcc aagggaagat
     4681 tcattcctgc aattacgtgc actctgtcag aaatataggt aaaaaacttt tgatttacac
     4741 ttcctccagc catgtcagtt aaaacaatta tttcgtctgt agtatccact aactctttaa
     4801 tttgttcatc tatatcatca atttcttcga catatgcaca taagctacta atttgattat
     4861 gcatacctgt taaaaactct agtgtctctt taaaaccctc agctagtatt gaatggctag
     4921 caatgatcaa tcttctcata attcaaatcc ttttccttta cgctaatatt ccaaatgcag
     4981 ccataaccat cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg atttttttct
     5041 tactgagtag ataatatgct gcagcagtaa taattactgg aattaatgtt ggtaaaattt
     5101 tgtccaacat tggttggatt ttcatactaa cttcaccaaa agtgaattta agtggcgttg
     5161 aaacactgat aactgaagca attaatgatc caactactgt tagccccaat actgaagcag
     5221 cttcagttaa ataattaatc ttttcaccgt attcagtaac aatcttcatt ccctgcttat
     5281 aaccaaattc aaagcctctc attcggaaaa agaagattac catgattaaa atgaaccata
     5341 aaaagattcc tacaggatta ttttctaaag ccatataagc cgcaattgat cccatgatgg
     5401 ttgggatcat cgtcatcaaa agagagtccc caataccagc aattggaccc atcgtaccaa
     5461 ctttaaagtc attaatcgca tcttttgctt ctaatccttg cttttcctcc attgcaacac
     5521 aagcacctaa aataatggct gccagccaag gctgtgtatt gtaatactga aagtgattat
     5581 ttaaagcttc gatgtattca tcatcatctt tgtataattt tcttaaaata ggtgacaatg
     5641 cgtaagtcac agatgcacct tgctgtgttt gataattaaa ggtacaaact gacatcaacc
     5701 aacgatagtt cgctttccta agatcctttt tcgttacttg atatttttcg ctcattttat
     5761 tcatcctccc caataaaccc aacatcagaa gctcccgcag aagcagtgat ttgtgcatct
     5821 ttttcagctc tatgatacac attaaatgct aaagcgaatc ctatgatcgc cgctccaata
     5881 ataggtacct tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact aatgtatcga
     5941 cctgtaggca taaagcgtaa caacatcgca ataccaacag caggcaacat ttttccggct
     6001 aatgttagcc catccataaa ccaattaggt aaaacatcca aaacgtaatt aacagcagac
     6061 tgtcctaaag taacggcaat aaatactgga attgctgcag ataaaccaaa gaaaattggc
     6121 cccaaccaca aaacactatt cattttctta aattctcgtt tattcgcata ttcctgtgat
     6181 ttgcgaacaa caaaaccatt taagattttg acaattacat ctagttgcac ccctaacata
     6241 cctacaggta gtcctattgc taatccggct tctactccaa gctggctacc aaatgccgtt
     6301 gcaataattg cgcacgttcc atagtccggt acagatgagc caccaaaacc ggcaactcct
     6361 aatgacatca attgaactgt accgccaact atcaaacctg tttttatatc acccatcaaa
     6421 attcctgtga ataatcccca aaaaactgcg taatttacaa agatcatcgg aatcccataa
     6481 taatcaacat gtttaataaa tgctaatata gtcagcataa taatttgaaa aatagtaaaa
     6541 tgcattatcc ttactccttt atcctaattt tgattgaatt tcctgtgcct tatccgccgg
     6601 aacaaattgt gtaacaattt ctacaccaga atccaacaat tttttatacg tgctgatatt
     6661 ttcatctgat gcataggctc tagaagatag ctgtaatgca ttatctttaa caacagtacc
     6721 gccaacaatt agtcggtcaa ttctaatacc gttatctact aaatgaagga ttgtttccgg
     6781 ctctttcacg atgacaaata ctttttcctg atcgtatttc ccctttttaa aattcgcaat
     6841 cgctgtttct tcagtgataa ttgatacagc cattccagca ggtctagcta aacgcatact
     6901 tgatttttgg atgtcgttat ttgcgacaga atcatcgatc accatcactc tgtttgcttt
     6961 agacattggt gcccattgcg tgacaataat cccatgcaat aaacggttat caactctcga
     7021 cataacaata ctcataaata cttcctcctt tatctttcat tttcttacaa gcaattacca
     7081 tgccaaaaca aaaagaactt gataaatcaa gctcttttta cataatcata aacgtattta
     7141 acctcatgaa tcgggatgtc gacactataa taatgttcaa cgacactgaa agattgctta
     7201 aaatgtgtct gaaatttttg aaattcctca ctcgtttcat tgaaataaat ttcatcttcc
     7261 aaagtatttt gttttactaa tcgttcaatt aagcaactaa tgtgaacata taatccgaaa
     7321 cacgtattat tatcaaatga aattcctaga tcattttgta agatgtatat agcatctgaa
     7381 acccgttcta ataatttagt cggatttaat atggtcaatt gctccattaa attcgttaga
     7441 ctaaaattct tcagaatatt attgttcaaa actttcagtt cattatcatt aaagtgtcta
     7501 ttcaattgat tggagaattt acttatattc attcctaaaa tcatatcttc aattgaaaag
     7561 aattctacat cttctagatg tggatttagt gttccaacaa taaaaagaat attgttctct
     7621 ttacgaagct ccgataaata gttttcattc actaaagagt aatagtcaca cgtttctatg
     7681 acaagatcag tatcccttgg aaagctatct tcaagtatcc tttttaattt aaacgaagtt
     7741 cctaatccgc ttgcacaaga acagacaatc atatccttct taatatttga tgattcataa
     7801 atataatcca cattttgatt ttcttttact tgttcaagaa tctgtttaag cggtaaatct
     7861 aataatatct gatttgcaac atcaatagcc attcttgttg aaacattgtt tagtagtccg
     7921 aatttttttt gagcgtctag ttccaaattt agataaattt tctccagtga acccatatca
     7981 acaagtaaaa taatttcttc atatccggta cactcttgta agtatctatt aattttgtct
     8041 gcaatctcac tacttgaagt cattagtggc atatcaatgg cgtcgtatat atatctctca
     8101 acgatcctat tagcagtact tgctatacag gttgccgtta gatctccatg agccaaaatc
     8161 agtgctaaac gtttttgctg aaattcttta ctacacatta acttaaataa aataaccaaa
     8221 acattttttt gaatttttgt taaataaata tcccctattt gaataaaacg attgactaat
     8281 tgaacttctc gactacacca actattaata tcattgatga aggaatataa tttatcgtcc
     8341 gatgttaatt ggatttgata aatccaagaa ttcaaaaaga tagacgttat ttgaagataa
     8401 taatagtcaa attctatttg aaaatctctc tctatttcct taacatactg tctaaatcga
     8461 atctcttgat aggtgggata tgttaaagct gaattatttt ggatatcaac attcttttgt
     8521 ctaaaatatc tatcatattt ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt
     8581 tgataaagat actctataag ttcgttgata ttaaaaactt cgtctacttt ttccaaatca
     8641 tccatttgaa tatattttaa ttcattctgt cttttcataa ccttcatgcc gttgaaattc
     8701 acccattgtg gtaaatgata catctgaata taaagttctt ccaaatcatc taagaaataa
     8761 aaggcattgg cacaggtcaa ttgaatgata tttttcaaat tgccaacatt ttcactaaag
     8821 cgatagttta ataaaaaatc taataattgt tgagatatca gtattttctt tgctagtctt
     8881 tcttcttctg atttaaaaaa tatcttgatt aactgttttc tttcataaag agaacgactg
     8941 tctaatgttg gaagtgttag ctttacaggt attcttctca acaaagttga taatagttta
     9001 tcctcaggtg gttctgatgt tgcaaatata atccagcaac ttgatgtgta ccagttttca
     9061 ttatcaccca tccgatgaaa tttcctcgta tccataaact gaaaaagttt ctcctgacat
     9121 tcattgctca atgcatgaat ttcatctaaa aataagactc cattattagc taattctgct
     9181 aaaccctttc ttccatcttc ggctcctgta tatgcacctt ttacatgccc aaaaaaattg
     9241 gacaagaaaa gttctggatt attggcatat tcactacaat taactgaaat gaaagatgcc
     9301 ttctcttcta tataattatt ggccaacaaa tccttataca cataatttgc taaatagctt
     9361 tttccagttc cagtctcgcc atgaagtaaa atgggtaagc catttggcgg atagtagatt
     9421 gctgctttaa tttgagcaat tacatttcgc agacttcctt gacttccgat aaccctttct
     9481 attccaaccc taaaagagct atcataccat ttttccgctt cctctgaaga gctaaaaatt
     9541 tgttgatttg gaactttctc cggtaaagaa aagaagatta ctggacgagt attgattttt
     9601 ccaaattctc catcttttac gaattcattc aaatattgag agacaatatt acgactaaca
     9661 tttaattttt cagatatttt ctgtgctgta ttgttccccc atttattttt aatctcagag
     9721 ttctctatga cataattgac aatgcgttct tttaattctt ccatcacagc acctcctcga
     9781 cactattcga attttgccga cagtattttt ccataaatat gacttgctaa aaaggctttg
     9841 tttagatcat tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga
     9901 tactctggcg aaatcgcata ccccaaaata tcaacgattg ttgcaatcag atcttctttt
     9961 gattcttcat ctgaaagtaa ttcacgatac aacatcgttt gtttgcttac tgtgtcatat
    10021 tcctctaagg attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat
    10081 ttatagaaaa tcagccccaa taaataattt ttatattctg acgcatccat tttactacgg
    10141 agattatctg ccgcactaaa taacttctga tttaattctg cacccaaaaa gatacactcc
    10201 tatctcttat ttaaagttgc tatataccct cttattttag catactcagt tttagaaaaa
    10261 gcaaaaaaat aaagaaactt attcttgata acaagctacg tgacgtatat ccctaacaca
    10321 aaaatagcca tatcctttgg tttggatacg gctatttgat attactcatt caattatcaa
    10381 atcacagtag gtgtgaatct catcgggatc ggcaatatct acttctgatt aggatcaaag
    10441 gtgattttct caataaaggt gtgatacaaa gctttaactt gcttcttctc actctggata
    10501 agaaagcaat ccaaactagt caatatctgt tggacatctt tgacttgaat cggttcccct
    10561 tgttgttgaa agatccctaa gatttcattc tctcgaattt gtaactgtct tcgtttttca
    10621 gtcaattcat ttaaacgatc ttttaacatc ggaaacaatt cgggactatc ttccaaagca
    10681 gcttcctgtt tttgctctaa tagttagatt ttgcgtacca ttttcttctt ttattttcct
    10741 tcgattgctg attttaatat agtctaaagg aaatttatat ttattgtatt ctttataact
    10801 tcaagagaac ttcgtttgag tattactgta tttttttaat tccataagaa aattagttat
    10861 tacctgccac ctaatcagca aatctgaacc tgagtcaata taattaatat tattttccat
    10921 ctccaacatt gtaagtactg ttggtgttaa tatagggact ttgtacaatt gtattttttt
    10981 gattttatca tctgcacaaa ggagattttt taaatctaat ccttctttta tttcaaaagt
    11041 tgaatttttt aaatctataa gagtaaaacc aattccttca tactgaattt ctgaaaatcg
    11101 aatttttctc ccagttgata gatcaagggg cagaaaacct tttattacat tgtgagtgtc
    11161 accatcattt acaatgtcat taggaatttt gattttagaa ttctgtggag cacctattgg
    11221 aagataataa ttcattataa ttttgtagtc ttcatgagga aataaagtac ttttaggatt
    11281 gctaagatat tctcgtaccg aatctaagaa tggcttatcc attgaatctt catccccatt
    11341 taaactaaaa aacattccta caattgcttt tgcaactcta agtgaattca ttttctttat
    11401 tgaatgtgtt aaatcagact taccattctt taaacttgta ttgttataaa ggaaattttt
    11461 attgtcttct gcaaatctaa tatattcctc aacatactca ctaccaaaaa aactattaca
    11521 tttactacat aacgtattgg aatggcaaca gtttttttga caaattttat aaggtgcaga
    11581 acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat
    11641 cttcggcagg agctaacagt taaagttaga ctgcctcgct agcgttagca catccgagct
    11701 cattatcaag gattcgctgc gccaatttct ggttacggta accaaccggt gtctcgtagc
    11761 caagtgtacc gtgcaaccga aggtggttcc accaattgac atagtcaaat aactccaaat
    11821 ccaattgttg taaggtttca aatgtgtatt gatagacaaa ttctactttc aacgacttat
    11881 aagttgattc agctacggca ttatcaaaag gacagccttt atgactcaat gatcgattga
    11941 tgtcaaaagt tgttaataat tcatcaatag cttggttatc aaactctttt ccacgatcag
    12001 tatgaaaaat ctcaacctct gtcagaggtt gtttgatacg gctaaatgc
//
LOCUS       DACCZY010000090        11981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCZY010000090 DACCZY010000000
VERSION     DACCZY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.091"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(123..398)
                     /locus_tag="IVZ67_002067"
     CDS             complement(123..398)
                     /locus_tag="IVZ67_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8166133.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(641..1951)
                     /locus_tag="IVZ67_002068"
     CDS             complement(641..1951)
                     /locus_tag="IVZ67_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8166134.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2133..3362)
                     /gene="rpsA"
                     /locus_tag="IVZ67_002069"
     CDS             complement(2133..3362)
                     /gene="rpsA"
                     /locus_tag="IVZ67_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8166135.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3497..4177)
                     /locus_tag="IVZ67_002070"
     CDS             complement(3497..4177)
                     /locus_tag="IVZ67_002070"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8166136.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4226..4861)
                     /locus_tag="IVZ67_002071"
     CDS             complement(4226..4861)
                     /locus_tag="IVZ67_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8166137.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4927..6330)
                     /locus_tag="IVZ67_002072"
     CDS             complement(4927..6330)
                     /locus_tag="IVZ67_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8166138.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(6317..7348)
                     /locus_tag="IVZ67_002073"
     CDS             complement(6317..7348)
                     /locus_tag="IVZ67_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166139.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7379..7600
                     /locus_tag="IVZ67_002074"
     CDS             7379..7600
                     /locus_tag="IVZ67_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8166140.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7765..8502)
                     /locus_tag="IVZ67_002075"
     CDS             complement(7765..8502)
                     /locus_tag="IVZ67_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8166141.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8655..9995)
                     /locus_tag="IVZ67_002076"
     CDS             complement(8655..9995)
                     /locus_tag="IVZ67_002076"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8166142.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10232..11755)
                     /locus_tag="IVZ67_002077"
     CDS             complement(10232..11755)
                     /locus_tag="IVZ67_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8166143.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         3389 a         2595 c         2012 g         3985 t
ORIGIN      
        1 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
       61 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
      121 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
      181 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
      241 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
      301 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
      361 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
      421 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
      481 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
      541 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
      601 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
      661 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
      721 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
      781 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
      841 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
      901 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
      961 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
     1021 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
     1081 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
     1141 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
     1201 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
     1261 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
     1321 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
     1381 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
     1441 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
     1501 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
     1561 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
     1621 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
     1681 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
     1741 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
     1801 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
     1861 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
     1921 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
     1981 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
     2041 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
     2101 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
     2161 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
     2221 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
     2281 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
     2341 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
     2401 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
     2461 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
     2521 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
     2581 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
     2641 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
     2701 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
     2761 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
     2821 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
     2881 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
     2941 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
     3001 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
     3061 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
     3121 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
     3181 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
     3241 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
     3301 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
     3361 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
     3421 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
     3481 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
     3541 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
     3601 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
     3661 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
     3721 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
     3781 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
     3841 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
     3901 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
     3961 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
     4021 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
     4081 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
     4141 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
     4201 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
     4261 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
     4321 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
     4381 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
     4441 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
     4501 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
     4561 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
     4621 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
     4681 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
     4741 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
     4801 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
     4861 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
     4921 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
     4981 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
     5041 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
     5101 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
     5161 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
     5221 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
     5281 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
     5341 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
     5401 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
     5461 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
     5521 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
     5581 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
     5641 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
     5701 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
     5761 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
     5821 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
     5881 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
     5941 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
     6001 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
     6061 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
     6121 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
     6181 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
     6241 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
     6301 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
     6361 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
     6421 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
     6481 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
     6541 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
     6601 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
     6661 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
     6721 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
     6781 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
     6841 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
     6901 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
     6961 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
     7021 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
     7081 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
     7141 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
     7201 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
     7261 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
     7321 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
     7381 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
     7441 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
     7501 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
     7561 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
     7621 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
     7681 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
     7741 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
     7801 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
     7861 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
     7921 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
     7981 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
     8041 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
     8101 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
     8161 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
     8221 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
     8281 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
     8341 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
     8401 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
     8461 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
     8521 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
     8581 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
     8641 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
     8701 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
     8761 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
     8821 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
     8881 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
     8941 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
     9001 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
     9061 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
     9121 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
     9181 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
     9241 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
     9301 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
     9361 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
     9421 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
     9481 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
     9541 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
     9601 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
     9661 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
     9721 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
     9781 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
     9841 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
     9901 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
     9961 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    10021 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    10081 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    10141 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    10201 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    10261 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    10321 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    10381 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    10441 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    10501 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    10561 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    10621 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    10681 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    10741 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    10801 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    10861 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    10921 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    10981 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    11041 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    11101 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    11161 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    11221 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    11281 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    11341 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    11401 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    11461 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    11521 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    11581 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    11641 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    11701 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    11761 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    11821 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    11881 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    11941 ttcctttttt ttataaacaa acattttcgt cttttaaaag g
//
LOCUS       DACCZY010000091        11785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCZY010000091 DACCZY010000000
VERSION     DACCZY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.092"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            369..638
                     /gene="rpsN"
                     /locus_tag="IVZ67_002078"
     CDS             369..638
                     /gene="rpsN"
                     /locus_tag="IVZ67_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8166144.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            653..832
                     /gene="rpmF"
                     /locus_tag="IVZ67_002079"
     CDS             653..832
                     /gene="rpmF"
                     /locus_tag="IVZ67_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8166145.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            864..1241
                     /locus_tag="IVZ67_002080"
     CDS             864..1241
                     /locus_tag="IVZ67_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166146.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1262..1411
                     /gene="rpmG"
                     /locus_tag="IVZ67_002081"
     CDS             1262..1411
                     /gene="rpmG"
                     /locus_tag="IVZ67_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8166147.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1435..2418
                     /locus_tag="IVZ67_002082"
     CDS             1435..2418
                     /locus_tag="IVZ67_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8166148.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2448..2570
                     /locus_tag="IVZ67_002083"
     CDS             2448..2570
                     /locus_tag="IVZ67_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8166149.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2588..4111
                     /locus_tag="IVZ67_002084"
     CDS             2588..4111
                     /locus_tag="IVZ67_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8166150.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4135..4374
                     /locus_tag="IVZ67_002085"
     CDS             4135..4374
                     /locus_tag="IVZ67_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166151.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4567..5028)
                     /locus_tag="IVZ67_002086"
     CDS             complement(4567..5028)
                     /locus_tag="IVZ67_002086"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166152.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5223..5441)
                     /locus_tag="IVZ67_002087"
     CDS             complement(5223..5441)
                     /locus_tag="IVZ67_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166153.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5559..7175
                     /locus_tag="IVZ67_002088"
     CDS             5559..7175
                     /locus_tag="IVZ67_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8166154.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7416..8756)
                     /locus_tag="IVZ67_002089"
                     /pseudo
     CDS             complement(7416..8756)
                     /locus_tag="IVZ67_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9032..9187)
                     /locus_tag="IVZ67_002090"
     CDS             complement(9032..9187)
                     /locus_tag="IVZ67_002090"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8166155.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9483..9815)
                     /locus_tag="IVZ67_002091"
     CDS             complement(9483..9815)
                     /locus_tag="IVZ67_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8166156.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10329..10520)
                     /locus_tag="IVZ67_002092"
     CDS             complement(10329..10520)
                     /locus_tag="IVZ67_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166157.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10614..11000)
                     /locus_tag="IVZ67_002093"
     CDS             complement(10614..11000)
                     /locus_tag="IVZ67_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8166158.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3937 a         1831 c         2254 g         3763 t
ORIGIN      
        1 gaataatcaa agttttaaaa aattggcgat aaaccagtta aacataaaag attgaataaa
       61 aagttatagt ttctttcata ttgccgtttt aagtagagga aaagctactt aaaacggtat
      121 ttgtgtttga ttggactaat aaaagtgaat tcttattttt cagagcctaa actgcatatt
      181 cttttaaatg atttgattat ctctatgtac cgataaattc gttttcaaat agttatctat
      241 tcatatgagt tttatttcat gttatttatt gttgacaaga gcaactcttg ttggtaaagt
      301 atcagtgtca aaacgtaacg attacgattt gaggtgtcta aatcaaaata aaaggagtat
      361 aaaggtatat ggcaaaaaag tcgaaaattg ctaaagcaaa gaaacaaatg gcgatgattg
      421 aaaagtatgc agataaacgt caggaattaa aagccgcagg agatcgcacc gctctcgcta
      481 aattgccaag agattcaaat ccgaatcgtc tgaggcttcg tgatcagacg gatggtcgtc
      541 caagagggta tatgcgaaag ttcggtatgt cccgtattaa gtttagagaa cttgcccatc
      601 aagggttgat tcctggagta aaaaaagcaa gttggtaaaa ggaggaaata tcatggcagt
      661 accagcaaga aaaacatcta aggcgaagaa acggttaaga agaactcatc aaactgtagt
      721 aaaaccagaa atttcatttg acgaagcgaa tggtgattat agacgtagcc atcatgtatc
      781 tttaaaaggg tactataatg gaaagaaagt aattaaagga gcttcaaaat agtttaatat
      841 ataactttta ggagggaatc aaaatggaaa ttatttaccc acctttagtt gaagaaggat
      901 taaagtatta tcttgaaacg acccagcaat cgctagataa atgcactttt tatcgttcga
      961 tggtagagag aggaattatt actgaaacgg gtttaccgac ccaacaggca attgaaaatg
     1021 gattagtcaa agattactat gaggaccaag gcttatcatt tgatgatttt ttaagaatct
     1081 atccaatttt tgaagagtat gacgaggagt tatttcaatg tatcgatggt tattgggaga
     1141 tacctgttga tatgaaagaa aatttagttt cacaattgga atctggcgag ttaacttttg
     1201 aagatgcaca acaaattcaa gcatatcttg aagatcgata agtattgaag gaggaagatt
     1261 tatgcgtcaa aatattattt tggaatgtgt cgaaactgga gaacgtctgt atctcaccag
     1321 caaaaataaa cggaataacc ctgaacggtt agaattaaaa aagtattcgc caaaattgcg
     1381 cagacgggca atttttaaag aagtgaaata gctaagtggt aaaaaggtga caaaatgggc
     1441 ataccagtta caatcgtttc tgggttttta ggggctggaa aaacaacgct aatcaatcaa
     1501 gccttgcaag tctcacccta ccccagggaa gaaattatta taattgaaaa tgagtttggt
     1561 gaagttggga ttgatcacaa attattagtt catagcgagg aacgggtgtt acaactaaat
     1621 aatggatgta tgtgttgcag tcttcgcagt gatttattag caatattgat ttctttactg
     1681 gaaacaatag aagaacatca acaaccaatt tcacaaatca tcattgaaac tacaggtata
     1741 gcagatcccc aaccaatcat tcaaacactt ttaacagcgc ctcaactaag aggaagagta
     1801 tatatcgata gtttattgac tgttattgat agtgacagtt ttgaacttat tgaaagagaa
     1861 ccacaagccc ttaagcaact tgtcatggca gatcgcattt ttgtgtctag aaaacaaaac
     1921 ttgaatttat ctgcactaaa aaaattacag agagaaatca aaagtattaa ccctttatcc
     1981 gatattcgaa tgttcacaca tagtgaaacg attcaaaaaa aggattttta taatttgaaa
     2041 aaatttaatc atcccttagt ttttgaaagt gattcatcag aaaatcacta taatggcaaa
     2101 tactatcatc aacaccatca agtacatgag tttaaatcaa ttctcttaac ttcagatcaa
     2161 gacataaaaa aagatttttt actccaatgg attgactggc tgattttaac caaccaaggg
     2221 agcctgtatc gttttaaagg attgattgct ttacaagagc aggattttat agttgccatg
     2281 caagaggtaa acgaacaggt taattttcaa tacaccactc gacaaaaaga ccaaaccact
     2341 attattctaa tcggaaaaat gttggatgaa gaaaggatca aacagagttt tgataaatta
     2401 ctggcggaaa gcaaataatg ataagtagtg attgggggta atttctaatg gagaaaacgg
     2461 atatatctag tgcatatcga cgacttaaaa gcccgaatat taaaactaga aaaagggcat
     2521 taaaaattat caaagaacat aaacgaaata aaatgaagaa attagcataa ttaaggaagg
     2581 aaaaagaatg aagaaaatca gtgtaggact tattgggata gcagctctag gattattggg
     2641 agcttgctcc agcactaatg atgcaaaagt atcgaatgat aaagatggaa aattagaaat
     2701 tgtaacaaca ttctatccaa tgtatgattt caccaaaaac atcgtcggcg atgaagcgaa
     2761 cgtggattta atggttccag cgggatcaga acctcatgac tatgagccct cagctaagga
     2821 tatggcaaaa gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca tggaatcatg
     2881 ggtaccaaaa gctaaagaaa gttggaagaa agctgggcct aatgtagtag aaggaacaaa
     2941 agacatgatt ttattgccag gtagtgaaga agaagatcat gatcacggag aagaagatca
     3001 ccatcatgaa ctagatcctc atacgtgggt ttcacccaaa atggcaatta aggaagtttc
     3061 aaatatcaag gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat ttgagacaaa
     3121 tgctgaaaag tatttgacta aactgaaacg gttggatgct gactatacca ctagtcttaa
     3181 agaagcgaaa caaaaaagtt ttgtgacgca acacgcagcc tttggatatt tagctttgga
     3241 ttatggattg aaacaagttc ctattgctgg actaagtcct gaagaagagc catcttctgg
     3301 tcgattagct gaattaaaag agtatgttaa aaaaaataag atcaattata tttactttga
     3361 aaagaatgct aatgataaaa ttgcaaaaac tttggccaac gaagctggga taaaattgga
     3421 agtactgaat cctcttgaaa gtcttacaaa agaacaaatg gataatggtg aagattatgt
     3481 ttcagtaatg gaagacaatc taaaagcgtt agaaaaaaca actatggttg ctggtgaaga
     3541 ggtagttcca gaaaaagaag cgaaggatga aaagacagtt gctaatgggt actttaaaga
     3601 tgccgatgtg aaggatcgcg aattgagtga ttatacaggc gaatggcaat ctgtttatcc
     3661 gttgttaaaa gatggaattt tggatgaagt tttcgattac aaagcaaaaa tcaataaaga
     3721 catgacagct gccgagtaca aagattatta tactactggc tataagacag atattgatac
     3781 tatcaacatt aaagacaaca ccattgattt tgtagtcaac ggtgaacatc atcaatacac
     3841 atacaaatat gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg gtgttcgttt
     3901 taattttgaa actgatgatg caggtgctgg tcgttttaaa tatattcaat ttagtgatca
     3961 tggaattgca ccaagtaagg cagagcattt ccatattttc tttggtggag aaagtcaaga
     4021 aaaactctat aatgaattgc ataattggcc tactttctat ccagcttcat tgtctaaaca
     4081 tgaaattgct caagaaatga tggctcatta aaattcaatt tttggaggaa atacatggta
     4141 agaaaagtat tattgtctat ggattccaag aatattgatg cttatcggcg ttatcgattt
     4201 gcaaagcaaa tgagattaaa tgggagtaca ttagctgata ttgctgcaac tttacatgtt
     4261 agtattgaca cagttcatag agtattaaaa cgggatcctg attgttgttt tatcaaggtc
     4321 aatactggaa gaagtagacg tttagcaaaa cggcataata gcttggccaa ttaattgata
     4381 ataccagagt agatcatttt tcccttattt ggatttgatc tgctttttat tatgtcttga
     4441 attgtcaaat caagcgcaac actttatcaa agaagacttc ataaggtgtt ctccaatcta
     4501 aacactttct aggtctgtta ttcagttttt caatcatctg atttatcgtt gagttatcta
     4561 ccgtagctat ctctttcgat tttggcaagt attctcgaat gagtctattc gtattttaat
     4621 ttgttcattg ttgccaaggg catgaggatc tggaaaataa aagggcatat tattcaatgc
     4681 ttctgtaact gcgaaatgat tggagaattc ttttcctcta tctggagtga tcgttttgac
     4741 cgcctcattt ggaatactgc ttaaaagttc aatcattttc tcgctcacat aagcggcttt
     4801 ctttctgggg accttctttc ctgctaaaag gaagcatgat tttctgtctg tcaaagtcac
     4861 taaacaagta ccaccagttt tgccagctac aatatcggct ttccaatgac caaaatcact
     4921 tctatcatct gcggattttg gacggccatg gatcgaatga gtaaggcaaa tctttccacg
     4981 agactcgaca tgattttttg tatgtcttgt ttttcctcga tggtgcaatg aacggatcag
     5041 gcctttattg ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa ttgcacgtta
     5101 gatcgtgata aaactaattg aaaagtccat atgttcatgc ttcaaacgat tcgagatttc
     5161 ctctggcgac cactgaaaat cgacaaataa atgacgaaca attgaccaaa ctcacggatc
     5221 atttagaatg tgataacgtc cgcatttttt tcttcgataa gcataatttt tttccgcttt
     5281 ggaaggtgaa tagttttttt cttgtttctt ctcagttcat gtaaaatagt aaacgggcta
     5341 tgctgaatgt cgttgccaat tatactaata gaaaatcctt cttcgtgaag cagaaagatt
     5401 ctttctcgtt cgagtatagt aagatgacga tactggctca taattatttt ccttctagga
     5461 gtgttctcga caaacttata ctagttggat atcgattatg actcattttt atgtgttgca
     5521 cttaaagtgt aaattcaagg tatagaaaga ggttccctat gaaaaaaata cctattacaa
     5581 tcttaactgg ctatcttggt tcaggaaaaa cgatacttct aaatcatatt ctgtacggtg
     5641 atcacggaaa gaaaattgct gtcattgtaa atgacatagg ggaagtaaat gttgatgggg
     5701 tattagtcga caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg atgccgaatg
     5761 gatgcatttg ttgtacatta cgcgaagatt tgattgttga actagatcaa ttagtgcaat
     5821 tttctgactt gaatcaaatt gttattgaag cttcaggaat cagcaaacca attccaatta
     5881 ttcaagcaat tatattagct aaaaaccctt tagacattga tttgacccaa cgattagaga
     5941 ttggtgccat tattatagta gtggatgtaa aacgtttttg ggataatttc gaaacgggtg
     6001 aaaagattca ggaaagaaaa gctgaaggta atgtagttga agaagaacag gatattcgtg
     6061 gcttgttaat tgatcaattc gagtgttgta acatattact tttaaataat agtgacttag
     6121 tgtcaaccaa gacgatttac aaaattaacg gtctaattgc taagttacag tccaatgcca
     6181 aaataattag gacaattaaa ggtgaagtag acttatcgaa aattctaaat acaaatcttt
     6241 ttaatataga tgagacttcc tattcagtgg gctggttcaa agagttagag cttggctatc
     6301 aaaatcatat tcctgaaaca gaagaatatg gtatctcaag ttttatctat cgaaatcggt
     6361 tactccttga cgcacaaaaa tttgcccatc ttacggataa aaattttcct ggcaatatta
     6421 caagagcaaa agggtttctt tggtttgccc aacatagtga tttttcgacg atctttgaaa
     6481 catcgggtag ctataaaagt attgaatcat taaattattg ggtagctagt ttacctaaaa
     6541 ttgagtgtca aatgattata caaaaaaatc ccgaattgaa ggaagcctgg catccgttgt
     6601 ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa caatgtatta
     6661 aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc cgaaaaattt
     6721 caccatttcc atggaaaaag tctaatatgt ttagccagat atcgttaatg aaatttttaa
     6781 gaaaagctgg tgtcaagata ggcgatgtca taattattgg cattttgatt attacctctt
     6841 ttattccctt atttgtattc tctatagaaa aggagaatga aacaactgaa gatgctatca
     6901 aatatgccat cgttcgaatt gatggcaagg aaatggatcg cttcgatttg gataagatca
     6961 atcataaact cgtgaattat catcctgcga agggacaata taatattgtt gagattaaag
     7021 atggaagaat tcgagttaaa gaagataata gtcctgatca aatcgcggtt agaactggat
     7081 ggatctcaaa acccggtcaa acgagtattt gtttacccca taagttagtt attagtattg
     7141 agaaaaaaga aagcacagat tactacattt actaatctgg aaatagatga ggaggttttt
     7201 aataagaaaa agatattatt attaataata gcaggggctt tattaagtgt gttaagcgct
     7261 tgtggacaag aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg gacctgcaat
     7321 ggtttagtta acagtcggtt agattactta ttttggctct tcgtcaatga gtgttggtga
     7381 agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt gtctagccgt
     7441 gttgttgttc tggctttagt cagttgggta gtattcttag ttttcttaaa atattttcct
     7501 cgatataaaa agattcgttc tcggtagttt tgaaagtttc tatagccata ggcaattcgt
     7561 tttagtactt tgatatggtt attcaaacat tctatagggc cgttggaata gggaacgcgc
     7621 aacgctcgtt tgatttctgt ttggtatttc ttgaaggtcg tgaacactgg ttggtagtgc
     7681 tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac atcttgtctt
     7741 ctgataacgg aaagaaaatc ctggtagact tcgtagcctc gctttaagga atcatcataa
     7801 gataatagcc gatcgactat ctctgcttct gtcagatgat ctcgaaatga cggacgccag
     7861 tagcgatgct gacctttcaa ttcccacgca ttcttttgaa gtagtttcca gtatttcttt
     7921 aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat tcggtggttg
     7981 gtaaaagccc gaccaatgtg ttggctaata tgaaagcgat cggcgacgat tcgggcattc
     8041 ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac cactagacgc
     8101 acttgttcac ggacttttat agggtagcgc aagaaatagt cgcgtaaagg attgagttgc
     8161 cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac aaagctcatg
     8221 gctcccgaga catttttcac tgatttgaat tcatcgaaac acaccacttc tggtaacagc
     8281 tggctcaaag aaatggtttt aggccgaagg ctgcgaagta ttcgaatgac agtggtcgga
     8341 gaaatgtgct tcatacgtgc gatcaaagac atagaaagag gttcgcttaa tagctcaaga
     8401 atggcttgtt tgactctgcg agcaatggag cagcgacgat cactaactga atcttctgct
     8461 aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg cagaatggtc
     8521 ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccta tttaatgatc
     8581 gtttgttcat tttgatagtg gcgatgaaag cattcctccg gttgataggt caatgtgcca
     8641 tcaaaaacaa aacatgtttc gtcattgatt cgctctttgc gaaaggcatc ttcatgaaag
     8701 ataagattta ggtctagaat atctagtaac tctttggtaa actctgaata ggacatcttg
     8761 aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggattttaag gtgtctgttt
     8821 gcgtttatta gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac actagatatt
     8881 atagatccct tattttcttc gcttcaaatt gtcaaggagt catcataaat tttgtatgga
     8941 ttcttttgtt tgggtagtag tcttctaaat aatcttggat taacaccttc atctctattt
     9001 tacttttata tttgttggag tcgattaatt ctcatttgat tgatcgatga aacgcttcaa
     9061 ttactgcatt gtcaacagga gtcccagttt ggctcactga atgtgtgatg tggtggtcgt
     9121 tcaacagctc ttgataaata aaagaccgat gctggctacc tctatcggaa tgaattaccg
     9181 taggcaattc tccttaataa atttgtaatg gacggacgac aatctctttt ttcatgtgtg
     9241 aatctaccat atagtaacgt agaatacgag ttgctaagtc aatataggtg gacaaataga
     9301 ggcgaccgtc attacatgga atgtaagtaa tatccatcac ccaaacagca tcaatttggt
     9361 tctgttcaaa ttatcgattc aacatttttt taccatttct ttgattgttt tttctctacc
     9421 atgagaagat ttgcgtcgat gaaatcctct tgtgcataaa ttcatttcac gcataagaac
     9481 agctacaaca cgtttattga tttcgattcc ttcatcgtaa agtttttgag tgatccctgg
     9541 agatccgtat cgatctttgt gttcgtagaa cactcgtctt cctcttttag tcgtttattt
     9601 tcctgttcaa gaatgtagaa atctgcttta tcaggtaatc tttttccttg tccagcaaaa
     9661 gcatttttcc cgtatcgacg atattctgaa acccatcggg aaattgttgg acgagagata
     9721 ttgtattctt cagcaatttt cttggtagtt acttttttct caaggatatc ctgtgaaatc
     9781 tttagtttaa atttacgatc atatgttttt ctcaatagag ttcattcctt ccggtaacgt
     9841 aactgatagt caagaaaagt gtatcatagc ccaacttagt atcagggttt gtcaactaaa
     9901 ctgtggaagt tgatagataa gagtttttaa caaccaaaat tctcttgact atttttgaaa
     9961 tcagacaaat tttgattgga cacatagttg aataaatcta ccgtcgttac ggaaggtaaa
    10021 tcgcatgctt ttcaattcta gaggagaagt cctcgttgaa gtaccatagc ccagttctgg
    10081 attcgacctc ctgcccactt catacgtaaa taaagtaatt ttaggagtga gactgtgaca
    10141 aaagttattt ttaagctaaa aacgaattaa aaaacgttga taattaaaat cattcgtttt
    10201 agattctatg aatcattata gaacgctgta aaatcgatac tttataatta taaagaagat
    10261 agtttcaaat ttattgttcg aaatttgaaa ctatcttttt acttatgtca cagactccct
    10321 tagttgagtt attctttaat ttcgaatttc gagatagcta gatatattgc aagcatacat
    10381 tcttgataac aatcttgatc atattttcca gttctccagg attgcttatg aatcaaagag
    10441 ctaaatcttt tgagaattgc ctcaacagct tcttcatttc cagcctttgc taaaatgaaa
    10501 tcttctttta aagaattcat aaatctacct tcttcccatg gacttataat ctatttcttt
    10561 gggaaggagg agaacgagca aagaagcata caaaattagt tattatgcag atattaaacc
    10621 agcatttttt gaattttttt gtaaagtcga tgttttaaat ttgtcgcacc ctgtcttgta
    10681 atccccagaa cctttccaat ttctttatca gttttatcaa agataaattt ttcgataaga
    10741 aaaatttgtt caggttcttt caacaaagga aggatttttg ctaattttat atcttcaaca
    10801 aggagagtca catttaaaac attaatcggt tgtttaaatt ttatagtatc tttagtaaac
    10861 aaatatggtg ggagttgatc aagttgttca tgtttttgaa tttcttcttg ttttttataa
    10921 taagtattag cggtgttcaa aatgaccttt tcaaagaatc gaatcaactg ggcatttacg
    10981 cgttcgtcgt tatctgacat attgcacacc tcctccatta agatttaaag ggaaaagagg
    11041 caaaaaacac gaaaagaagc taatgcaatt atgctgcgct gcgaataact tctataagct
    11101 cacatgcatt gataggactg ctgatgttta ttttcaaaaa tgttttttta gttgataatg
    11161 gatagttatc tttatactta gaaaagaaaa ttcatatatc ttcttaaata tcaaacatgt
    11221 ccttatggct aagaaaatgt cttgagcttt ttacgtataa taaatctttt ctaaaacctc
    11281 cttttggata atattcttat tcgtgtatag attgtatttt ttatttcttc ccccttcata
    11341 attaacaact agaaaagagt tcgaatagac acgtatttga acgaaaattt ttattggatt
    11401 cccaaattgg aatatttaga aacgaaaaat tcctattatc tgtatagtgc ttagataata
    11461 acggatcata ataattattg tctttgaact agatatatgt aaatagtatt catcatgatg
    11521 aaaaatgatg agttctcggt gataaaatgt actttttacg gtggaatata catgaaagcg
    11581 ataagtggat attttataat gaagtaagtt agcttatttt gacagaggaa tagtatttta
    11641 gataacgtat aaaatactat ttaatgatgt agaaaggaaa gagagaaaca aaagtgtttt
    11701 cagataaaga agtcttatta aagtctcttc atgtttgttt aaagttacct atcgtggttt
    11761 ttaattccga attcagtatt ataaa
//
LOCUS       DACCZY010000092        11743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCZY010000092 DACCZY010000000
VERSION     DACCZY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.093"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            145..444
                     /locus_tag="IVZ67_002094"
     CDS             145..444
                     /locus_tag="IVZ67_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166159.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            517..1878
                     /locus_tag="IVZ67_002095"
     CDS             517..1878
                     /locus_tag="IVZ67_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8166160.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1900..2160
                     /locus_tag="IVZ67_002096"
     CDS             1900..2160
                     /locus_tag="IVZ67_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166161.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2174..2926
                     /locus_tag="IVZ67_002097"
     CDS             2174..2926
                     /locus_tag="IVZ67_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8166162.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3020..3625)
                     /locus_tag="IVZ67_002098"
     CDS             complement(3020..3625)
                     /locus_tag="IVZ67_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8166163.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3678..4652)
                     /locus_tag="IVZ67_002099"
     CDS             complement(3678..4652)
                     /locus_tag="IVZ67_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8166164.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5030..5809)
                     /locus_tag="IVZ67_002100"
     CDS             complement(5030..5809)
                     /locus_tag="IVZ67_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8166165.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5876..7147)
                     /gene="celB"
                     /locus_tag="IVZ67_002101"
     CDS             complement(5876..7147)
                     /gene="celB"
                     /locus_tag="IVZ67_002101"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8166166.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7300..8697
                     /locus_tag="IVZ67_002102"
     CDS             7300..8697
                     /locus_tag="IVZ67_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8166167.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8778..9218
                     /locus_tag="IVZ67_002103"
     CDS             8778..9218
                     /locus_tag="IVZ67_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8166168.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9366..9692
                     /locus_tag="IVZ67_002104"
     CDS             9366..9692
                     /locus_tag="IVZ67_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8166169.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9693..10442
                     /locus_tag="IVZ67_002105"
     CDS             9693..10442
                     /locus_tag="IVZ67_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166170.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10522..10977)
                     /locus_tag="IVZ67_002106"
     CDS             complement(10522..10977)
                     /locus_tag="IVZ67_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8166171.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11404..11679)
                     /locus_tag="IVZ67_002107"
     CDS             complement(11404..11679)
                     /locus_tag="IVZ67_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166172.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3720 a         2034 c         2376 g         3613 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaga aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga
      181 gtagcaacga gtcagacaat cgctagtaag atccaaagta tgttagaaga tgacggcatc
      241 gcatttcctg tagaagcagt tgattacaaa tcgattcaaa atgagcttct aactgctgga
      301 atctatgtgt atgtcgcaca acctgatgag gaagtacttg aacaagcaaa agatttgggt
      361 attgaagtat ttccaggaat tccattttta actggaatgg gtgtagaacc tatttatgac
      421 agtatcaaag agttagtaca gtaaaggagg attgttggat aaaaaagttg gttcatagtt
      481 ttatttacta tatttagaaa gaaagaaggt ttatttatgg aagttttcca aaccattatc
      541 aattatgttt tgaacttagg atcagccatt tttgttcccc tgattatttt gatacttgga
      601 ttgttggcag ggatgaaatt aaaaaaagca ttcatgtctg ctttgacttt agggattgct
      661 tttagcggaa tgtctatggt cattggctat atgtctaacg cagtaagtcc agcatcagaa
      721 gcattggcta aaaatacggg aatcagtttg cctgcacttg atcttgggtg gacaggtgca
      781 gcaagcatca catggtcgtg gagttatgct tttatatttt ttgcagtaac gattggggtc
      841 aattttgtac tgttactttt taattggacg aagacgttaa atgttgatat gtggaatgtt
      901 tgggggaaag ctttaactgc ttatttagtt tattatgttt ccggtagttt ggctgcaggc
      961 tttcttactg caatggtcca agttatattg gaattaaagc ttggtgatat gtttcaaaaa
     1021 catatacaag atctcacagg gattccatta gtaacagtga ctcattttat gacatctgcc
     1081 gctgtcttgt tgctgccttt caatatgatc atggataaaa ttcctgcttt aaataaacga
     1141 gcggatacga atgcattgaa gaagaaaatt gggatcttct ctgaaaactc tgtaatggga
     1201 ttcattattg gattggggct tggctttgct gctgcgtatg gcgtttcagg ttctcttaat
     1261 ctagcgatcc aaacagctac agcattaagt ttgtttccta tgatctctaa aatgttcatg
     1321 caatctttgt ctcctctagc agacgctatg tcagaattta tgaaaaagcg gtttaaggat
     1381 cgtgaagtat atatcggatt ggactggccg atattagctg gtcgttcaga aatttgggtt
     1441 actgctattt tacttgttcc tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa
     1501 gtattgccat tagcagggat tatcaactat tcgattgcgg tcggcggatt acttttaaca
     1561 ggaggaaact tggctcgtat gctgatcttg ggaatcatca ctatgcctat ttatttgtac
     1621 ggggccactt accttgcgcc aatactcagt gatttagcag aaaaaagcgg agcagtcgag
     1681 ggaatcaaga aaggacagtt gatcacatgg tcatccatag aagggccgga gttcagggtt
     1741 ttatttgcgg aggcatttaa taggaatatt ctcgctattg taggatgcgt gatttttcta
     1801 ggactgtttg ttctacttta taaatatatg ataaaagcga aagtaccgag tgaacgttat
     1861 gctgaactac aaaaatagca ttgaaatggt gggaaagtaa tgaaaagaga agagacagat
     1921 aaaataaaat ggacagtcgc tctttgtggt acacttctgc tatttctata tggactcttt
     1981 actcagaata tcatcatcaa tctattggtc atttttttcg cactagtgat ctataaatat
     2041 ggaaatcacg ttttattcag agagtacgat gaaaaaagaa aacggaaaat agaagaatca
     2101 atgaaaataa aagaagctac caaagaaata ttgagagaaa aaagttttat aaaaagataa
     2161 tggaggggtc attatgtcag atggacgtat gttatttgag agagaacgac aggacatggc
     2221 acgtatcgta agagtgattt tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc
     2281 ctttaaaacc actgatgatc aagggaaaga ctacatcatc atgacgccga ccatgatgtc
     2341 agaagcctat ctaggagaga tttctcctgc acaggttcta gtagtagaac caagaacgcg
     2401 tagaatcgtt gacggagtcg gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta
     2461 tgagactaat ccagctatca aagctgtcct acatgctcat gcgcctaatg caatgttctg
     2521 ggcgactact gggttgccta tgcctaattt gacagaagcc acagctaaag taaaagagat
     2581 tcctgtcatg gactttgagc ctaattgctc tgaagaactt gctgaattga caagcgacta
     2641 tgtaaaaaaa cagcatctcg aattaccgca tatgttgctg ctcaactctc atggcgtatt
     2701 gatcaacaca ggtggagaat caggaatcga agctatccat aaagcactgc aaatccttga
     2761 tacagttgaa tggaatgcag aaattgccta caagcagacg atttttcaag cattagggat
     2821 tactgacggt ttttattcaa aaggtgagaa aatcggggga ttggaagagt tgaaacataa
     2881 aacggctatt tataataaat ctgttattgc agctggtgga gactaaccta tctgacacaa
     2941 aaaactacga ctttcacaaa atctgatcca gatcgatatc tgcttcaggt tgtattatgt
     3001 aagtcgtagt tcttttcttt tatttagtta agaggtggtc tgtccattcc ctcgcttgct
     3061 tgatttcgtc catcgtcaac tgatgacctg ttacagttag ctgaatagtc acatttgctt
     3121 ggcgtgtttc gaattgatcg gccaattctt taaaggaagc aggggaaacg attggatcgt
     3181 tatcaccata agaaatccag acggttttat cctctaatgt ggaacggctt tgatgaacac
     3241 ctagagacat cggatggaaa aacaaccctt tatttagctc ggtctcacgc tccaataata
     3301 aatgagcagc gatgtttgcg ccatttgaat agccgacaag cagccagtca cttaacggta
     3361 tcagttcttt ttcgctgatt tctttgattt tttcatggag tttatctgtt tctttttcca
     3421 aactgtccag gtcgaattga ttcaatccat tacgcttgaa aaaacgattc atcccatctt
     3481 cttggaccgt gccacgaaaa gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat
     3541 ccagtaaaga atgttcatcg ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg
     3601 ttcctttttc aaaaatgtag gtcattttgt tccctctttt ctgtttctat agatgcaaat
     3661 gaagtagcat gaagagatta ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg
     3721 acataagctt caatggcttc acgacgttct tctaagaatg gaggaagaga tagtttttct
     3781 cctgctgatt ctgctggttc atcttcaagg aatccaggac catcagttgc taattcaaac
     3841 agtacgtgat gagcggctag gaagtactca gattggaaat agaaacgatc gacgaaacca
     3901 gaatttggca attcaatctt attgattact tgaatccagt attccaatgc ttctttgtct
     3961 gctacacgca atgctaagtg atggatattt ccaaatcctt cgattgcttt tggcaagtct
     4021 gtccgatgtt caataatgat gcttgcacca tttccccctt caccgatttc aaataagtag
     4081 aaatctcctg aatggtctgt ttctttgaat cctaagattt tttcaagaat caagcggata
     4141 tgctctaatt gactgacacg gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc
     4201 ggtacattgc tgtttttcca aggaatgcca gctgctacac cttgattgtt ttcgtcagag
     4261 atcaattgat aatactgatt atcgaaatct tcaaattcga tatatttttt gccgaaacgt
     4321 tcattgattg ctgaatgagt aacgtcatat ttttcaaaac gttccaacca aaagtctaat
     4381 gctgcgtcac ttggcacacg gaaagaagca cgagagatcg tatctgttcc tttttttcct
     4441 tttggctgat ttggaaaatc aaagaaagtc atatctgttc ctggcgcacc agtatcgtct
     4501 gtaaagtaaa gatggtaagt ttcgatgtca tcttggttaa ctgttttttt gattaagcgc
     4561 attcctaaaa tatctgtaaa aaagcggtag atttttgtag cgctagatgt cattgctgtg
     4621 acgtggtgaa ttcctctgat ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa
     4681 aagtaagtag ttgatttaga catagtatag agggtgcatc tctattttgc aattaatttg
     4741 tcttctaaag tatcaggaag gaatcaagaa tggaaaagag gagggacgga tcaataaaat
     4801 aaaaagactt acgactagct gtatcgattt caaaaaaact agctttaaaa agctaacttt
     4861 agaatcatta cgggtggtca taagtctttt tatgctgagt tattttttca tttttgagct
     4921 aatgcttagg atagaataga atgttgattt cacgatctct tcttgttact tgaaactgct
     4981 taccactgtc acgatctctt ggtaaacggc tttttcacaa cctctttggt taagttgaac
     5041 gcacatccag cacagaatag ttccgaacat caaatcggtt tcggctggaa gaatattcaa
     5101 tcggctgtcc atctttgaga tagacgactt gctgaacttc cagtacagga tcgttttctg
     5161 cacagttcaa atattgctga tcatattcat cacttttatc tgcagaaata gcacgataag
     5221 cccctgcaaa atggacattc aattcttgat gaagatgatc atagatagag gctaacaaat
     5281 gttctttttt taaacccggc acaagatgga ctggcataaa ggtatgctct aaaacatatg
     5341 gctcgccatc taagatcctt aaccggtgaa tattgtagac aggttgctcg gcatcgatca
     5401 tcaatcgttc ttggatatat tgattaggga attcaacatc aaaatgaatg atttgactct
     5461 tcaaagaacg atgtctgcgt ttcatttgga gactcaaacc ttcatactcg ttagcgggcg
     5521 atgtgtcttt ttgcataaat ggatgattca agattttcgt tcctgatcct cgctgcgaat
     5581 agacaagtcc ttccattgct aagatattga tggctttttt gattgtcatc cggctcgcac
     5641 caaattcttt taccaagtcg acttgattag gcaaaaaact atcgagagga tactcttttg
     5701 aacggatacg ttctcttaaa atatttgcga tttcttcgta ttttggcacg ttattcacct
     5761 cttctagagt ataacgaaag attatgcttc cgacaactcg aagtagcatc tggaacattg
     5821 tttattcgaa aaaagaggtc atgacaagtt tttgtcacaa ccccttaatg atctattaag
     5881 caatatctaa atctggttgt ttatctagta ctttcaggaa tgggtaccag ataaaggcaa
     5941 cgatgacaaa gtcgatgact tgcagcatac caccaagcca tgaatttgtt gctaagacac
     6001 cgcttgcaat gattggcaca gtccatggaa cagagacgcc agttggtgca gggacaagtc
     6061 cagcagccat taccaaataa ttgaacgtcg ttacgattag aggtgccaca acccaaggaa
     6121 taaggattgt tgcattcaat acgatcggta gaccgaagat cgctggttca tttacattga
     6181 aaataccagg tgctaaagct aatcgtccaa catcacgata ctgtttcttt ttaagaacaa
     6241 aggccatcaa taaaacgaca gctaaagtca ttccagagcc accaatacca acagtgaagg
     6301 tttccatgaa tggttttgtg atgatgtgtg gtaattcttt tccatgttga taagcttcta
     6361 agttatcaag catcagtgta ttccaaatcg ggtccattac agagttgacg atgatttgtc
     6421 cgtgaagtcc gaagaaccat aagaattgga caaagaacaa agcaatcaat gttgcaggta
     6481 aactacttcc caaaccagtt aatggttttt ggatcacttc ataaacgacg tcatgtaagt
     6541 tagcattgaa aataccagtc atcacagcat tgattactaa gaaaatagtt agtgtcacta
     6601 cagcagggat caaagcggca aatgattttg ttactgcagg cggaactcca tcaggcattt
     6661 tgatctgcca gccgcgtttc gtgattctgc agtagatttc tccagccaag aaagcagcga
     6721 tcattccaat gaacattcct tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat
     6781 cttcaccgcc agcggttttc attgcaaaag gcgtaagaat caagaagctg gcaaaagaca
     6841 cggcaccacc gaaaatacct tcaacgtcat aagattttga taaataatag ccgataccaa
     6901 aagtaacaaa aaccgacatg atgctcattg ttgcattttg cccattaccg aataaattcg
     6961 ataaagttcc ctttgttgcg tcactgaaaa aaggcaagtt attgataacg acaacgatcg
     7021 aaccgaacat cgttaaaggg aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac
     7081 gattttgccc taataaatta gctaagggca tgattttctc agaaagcttg tccataaaac
     7141 cgttcatttt tattcacctc aaatttattt gcaatcgatt acatacaaag tataacgcca
     7201 aaaattttaa atgtctagtc attttcataa aaagtattga tttatttttt agggttcgtt
     7261 atgattacgg tatcaagaaa aaagagagga agtaaaaaaa tgaaaaccta cacattccca
     7321 gaaaattttt ggtggggttc tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat
     7381 gatggtaaag gagaaaatat ttgggatcat tggtatgaat tagagcctga aaaattcttc
     7441 gatcaagtag gacctgaaaa aacttcgcaa gtttacacaa aatacaaaga agatgttcag
     7501 ttaatgaaag aaactggcca taacacattt cgtacatcta tccaatggag tcatttgatt
     7561 ccagaaggga caggtgaaat caatcaaaaa gctgtttctt tttatcgaga ttattttact
     7621 gaattgctgg ataatggtat tgaacctttt gtgaaccttt accatttcga tatgccacta
     7681 ccactgcaag aaaaaggcgg atggttatca agagaaacgg tcgacgctta tgtttcttat
     7741 gcagcagcgt gctttgaact gtttggagat ctggtcaaaa aatggtttac tcataatgaa
     7801 ccaatcgtac ctgtagaagg aggctacttg tatcaattcc actatccaaa tcatatcaat
     7861 atgaaagaag cggtccaagt aggctatcat gagaatctag caagcgcaaa ggtaatcaaa
     7921 gcctaccatg aaggcggaca tgatggtgag atcggaatca tcttgaatct gacaccaagc
     7981 tatccgagag atgaaacgaa cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc
     8041 ttcaaccgat cattccttga ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa
     8101 attgtcaaag aattggctat aatgccagag atccatgaag gagacctaga actcatccgt
     8161 gaaaacacga ttgacctgct aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa
     8221 gaaactccaa tcaatcacga acatagcccc atgccagatg attattttga taattacgac
     8281 atgcccaata aaaaaatgaa tccatatcgt ggatgggaaa tttacgaaaa aggaatctat
     8341 gatattttaa cgaatacaag agaaaactat ggtaatatca aatgctatat ttctgaaaat
     8401 gggatggggg tcgaaggaga agaacgattt gttaacagcg aaggaattat tgaagatgat
     8461 taccggatcg agtttgttac ggatcatttg aaatacgtcc atcaagcaat ccaagaagga
     8521 accaattgtg tgggttacca catgtggaca tgtatggaca actggtcttg gacaaatgct
     8581 tataagaatc gctatggctt tatcgctgtt gatttggcac aagacggtaa acggacgatc
     8641 aaaaaatcag gtcgctggtt taaaacggtc tctgaaaaaa acggatttga tgcctgatat
     8701 tttttgataa aacgttcaca aaatgtttgt tctaacactg taatcgtgct acactaacag
     8761 tgaaggaggc gtttacaatg gtagatactt atcgtaatat tttagtagca attgatggtt
     8821 ctgaaaaggc agaaaaagca tttcttgagg caatcacgat tgccaaacga aatcaggcaa
     8881 cgttgcacat cctgtatgtc aatgaagtaa caggcaatta ctttggtgat tttgcatttg
     8941 ttacaacaag ccttcaggaa gaattggatg aggttgcgga aaaccagatg aaagaacatc
     9001 gaaacttggc aattgaaaaa ggcctgacag atatcgagac atatgttctt tacggttatc
     9061 caaagacact gattgctaat ttccatgaat cgaaagaacc aatcgatctg attgtcatgg
     9121 gggcgacagg tctgaatgcg gtagaacgtg cactagtagg ttccactact tcctatgtag
     9181 tcaaccacgc atcatgtaac gtcatggttg taaaataaat aaattaaggg aacagaaagc
     9241 aaaaaagcat aacgcttctt ttgctttctg ttccttttat ttttttgtaa aaaattactt
     9301 tacctgatga agtaatcatg ctattctaat tacaacgaca gatggagtaa tatggaggaa
     9361 agaaaatgat ttcaagtgat ggaatacgcg gatacaatga tgcgattatt ttatcgatcc
     9421 ttaacaaaaa cgattcttat ggatatgaaa tttctaaaac gattcgtgag aggacaaatg
     9481 attcctatgt gatcaaagag acaacacttt actccgtttt tgcccgttta gaaaagaatc
     9541 aactgattgt ttcgtatcct ggagaagtga ctcatggaaa aaagcggaca tattataaaa
     9601 tcactgaaat agggcgtaat cttttagaag aaaaaaagca agagtggctg acgacgaaag
     9661 acgtagtcga tcaatttttg gagggaaatt aaatggatac aatccgtaat tatttagaca
     9721 gtctttttat cggagtacct caaagtaccg agatagataa gctgaaaact gatttgttgg
     9781 caaatatgga agaccattat catgaattga tgggagaagg aaaaaatgaa caggaggcca
     9841 tcggtacagt aatttcgaca tttggttcaa tcgatgaatt attagaagaa ctagatgtcg
     9901 aaaaaaaaca tcaagctgac gagacggaaa caaacacagc ttctatttat ttaagcgaag
     9961 cagaaaacta ctggaaagaa tacagagcgg caagtctcca agtggcatca ggtgttctat
    10021 tcattagctt atcttttgca agttttcttt tcttttgttc agcaggatat gtgttcatgg
    10081 gtatttcttg tctgattttc ggtatagcat tagctgtcgg tttttttatt gcttctggta
    10141 tgaaaatcac acgtttaaat cactttttgc accatcggaa aatacctgaa aaagtattag
    10201 cagaagcaaa agaaaaagaa gaagagtatc agcgaagttt tggcttctca ctgattgcag
    10261 gaattggatt atgtattttt tcacttttcc cacttttggc ttctttaatg tggtatatgg
    10321 atggtagtat cggtgcctct atattttttg ttacggtagg aacgggtgtc ttcttgatca
    10381 tttatggcag tctagtccgt cattcttatc gtcaattcac tcaaagcgct tattactggt
    10441 gaatgtattc agatttgtaa aaatattcta tttatccggc gtagaaaaaa agactgtgac
    10501 acgcttttgt cacagtcttt tttatatata ataatttaaa aattcatcat ctaaatccgt
    10561 agaattttct agattgctta cccatgtaaa atcgttatag acagatttca gttgacttct
    10621 gcgccattgt acagggataa aaagacgcat gctatcttca ttgaatccga ctaataattt
    10681 ctttccgtca gtaatgatgg gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac
    10741 aagctctgag gtcgtacatt ggtcgatttg gtctttcaaa tttttataat caatcgagcg
    10801 tttagctaat agatcgtcaa atccgttttc tgttaatgac aataaataaa atatctcttt
    10861 ccttgttaaa ggctgtttag gtgtgaaaat tcgttctctg taaggcattt gatggtcttt
    10921 cagccagttt gccatcagat ggtaactatg tgtgcgagaa gaatagatcg taagcataaa
    10981 aaaactcctt aaatagtcca ttaatttgtt agatctgttg agataaataa taggtaagga
    11041 gctgtgcata agcattgatc aaaacaaccc aaacatcatt agaaataact ttctaaagtc
    11101 ttaaacttgc gacgagaagc aactagacta atagaaaaaa tgtcttagcc atttggcttc
    11161 ctgtttgttt ttttgcttat gctgcagctc ctcgggaaag ggttctaaca atttgaagaa
    11221 ggttgatgat tgatctagtt taaattgtgt gaaatttgaa atctggtgtt tttactagat
    11281 gaatcaccta acaactatga ttataactta tagaacttct gttttgcatt cggttttgtt
    11341 atagttgtta taaagtttta ttttattaca gaacattttt aattaataaa gaacagttag
    11401 aagttaaaat tgctgattca catagagttt atgacttctt ggaaattctt tttcaatcag
    11461 ttttttcatc tgtttatgaa attctttttt ttcagtaaac ataaaggttt gggtatcgta
    11521 accttcttca tgtaggaaat agattattcc tttttctgtg ttttgcgtat aagcttttat
    11581 actcctgtct tttttgaaag aacagatcgt atagccatct atttggtctg gtagtcgttt
    11641 gattttcttc aaaatgactt ttttatcctc aagtttcatt tttttcacct gactttattt
    11701 ttttcatatg gaaatcatag cgcctttggt tatatgaaaa caa
//
LOCUS       DACCZY010000093        11662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCZY010000093 DACCZY010000000
VERSION     DACCZY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.094"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(289..774)
                     /locus_tag="IVZ67_002108"
     CDS             complement(289..774)
                     /locus_tag="IVZ67_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8166173.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1254..1895
                     /locus_tag="IVZ67_002109"
     CDS             1254..1895
                     /locus_tag="IVZ67_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8166174.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1914..2549
                     /locus_tag="IVZ67_002110"
     CDS             1914..2549
                     /locus_tag="IVZ67_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8166175.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2567..3382
                     /locus_tag="IVZ67_002111"
     CDS             2567..3382
                     /locus_tag="IVZ67_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8166176.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3701..5137
                     /locus_tag="IVZ67_002112"
     CDS             3701..5137
                     /locus_tag="IVZ67_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8166177.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5326..7758
                     /locus_tag="IVZ67_002113"
     CDS             5326..7758
                     /locus_tag="IVZ67_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8166178.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7988..9055
                     /gene="rlmN"
                     /locus_tag="IVZ67_002114"
     CDS             7988..9055
                     /gene="rlmN"
                     /locus_tag="IVZ67_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8166179.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9248..10351)
                     /locus_tag="IVZ67_002115"
     CDS             complement(9248..10351)
                     /locus_tag="IVZ67_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8166180.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10903..11451
                     /gene="aac(6')"
                     /locus_tag="IVZ67_002116"
     CDS             10903..11451
                     /gene="aac(6')"
                     /locus_tag="IVZ67_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8166181.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3880 a         1957 c         2506 g         3319 t
ORIGIN      
        1 aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag
       61 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
      121 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
      181 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
      241 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
      301 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
      361 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
      421 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
      481 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
      541 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
      601 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
      661 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
      721 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
      781 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
      841 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
      901 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
      961 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
     1021 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     1081 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     1141 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     1201 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     1261 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     1321 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     1381 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     1441 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     1501 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     1561 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     1621 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     1681 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     1741 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     1801 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga
     1861 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     1921 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     1981 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     2041 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     2101 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     2161 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     2221 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     2281 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     2341 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     2401 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     2461 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     2521 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     2581 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     2641 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     2701 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     2761 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     2821 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     2881 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     2941 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     3001 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     3061 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     3121 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     3181 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     3241 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     3301 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     3361 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     3421 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     3481 ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta
     3541 tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact
     3601 ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta
     3661 aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac
     3721 ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc
     3781 ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa
     3841 gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga
     3901 tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg
     3961 gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt
     4021 gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc attgggatct
     4081 tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt
     4141 cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag
     4201 tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg
     4261 tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc
     4321 tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta
     4381 tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga
     4441 agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc
     4501 catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt
     4561 gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc
     4621 attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaagttc
     4681 atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg
     4741 aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac
     4801 aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt
     4861 gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa
     4921 agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta
     4981 tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga
     5041 tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt attactggta
     5101 ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg
     5161 tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg
     5221 taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct
     5281 tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa
     5341 cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa
     5401 caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta
     5461 aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact
     5521 taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca
     5581 gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc
     5641 ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg
     5701 gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt
     5761 ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct
     5821 ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg
     5881 gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg
     5941 gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag
     6001 cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga atatgagaag
     6061 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     6121 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     6181 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     6241 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     6301 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     6361 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     6421 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     6481 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     6541 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     6601 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     6661 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     6721 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     6781 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     6841 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     6901 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     6961 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     7021 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     7081 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     7141 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     7201 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     7261 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     7321 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     7381 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     7441 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     7501 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     7561 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     7621 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     7681 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     7741 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     7801 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     7861 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     7921 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     7981 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     8041 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     8101 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     8161 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     8221 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     8281 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     8341 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     8401 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     8461 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     8521 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     8581 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     8641 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     8701 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     8761 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     8821 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     8881 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     8941 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     9001 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     9061 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     9121 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     9181 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     9241 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     9301 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     9361 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     9421 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     9481 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     9541 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     9601 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     9661 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     9721 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     9781 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     9841 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     9901 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     9961 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
    10021 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
    10081 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
    10141 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
    10201 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
    10261 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
    10321 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
    10381 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
    10441 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
    10501 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
    10561 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
    10621 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
    10681 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
    10741 ttcctatttt gaataaagtg tttctgtata attttttatt ttataagtaa gataaataat
    10801 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
    10861 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
    10921 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
    10981 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
    11041 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
    11101 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
    11161 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
    11221 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
    11281 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
    11341 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
    11401 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
    11461 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
    11521 aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag
    11581 aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc
    11641 tacatttttg cataaatttg gc
//
LOCUS       DACCZY010000094        11130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCZY010000094 DACCZY010000000
VERSION     DACCZY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.095"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            4..741
                     /locus_tag="IVZ67_002117"
     CDS             4..741
                     /locus_tag="IVZ67_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8166182.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            755..1570
                     /locus_tag="IVZ67_002118"
     CDS             755..1570
                     /locus_tag="IVZ67_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8166183.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1583..2230
                     /locus_tag="IVZ67_002119"
     CDS             1583..2230
                     /locus_tag="IVZ67_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8166184.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2246..2902
                     /locus_tag="IVZ67_002120"
     CDS             2246..2902
                     /locus_tag="IVZ67_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8166185.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3064..3885
                     /gene="racE"
                     /locus_tag="IVZ67_002121"
     CDS             3064..3885
                     /gene="racE"
                     /locus_tag="IVZ67_002121"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8166186.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3888..5243
                     /gene="rph"
                     /locus_tag="IVZ67_002122"
     CDS             3888..5243
                     /gene="rph"
                     /locus_tag="IVZ67_002122"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8166187.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5244..5762
                     /locus_tag="IVZ67_002123"
     CDS             5244..5762
                     /locus_tag="IVZ67_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8166188.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5869..6369
                     /locus_tag="IVZ67_002124"
     CDS             5869..6369
                     /locus_tag="IVZ67_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8166189.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6649..7569)
                     /locus_tag="IVZ67_002125"
     CDS             complement(6649..7569)
                     /locus_tag="IVZ67_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8166190.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7734..8747
                     /locus_tag="IVZ67_002126"
     CDS             7734..8747
                     /locus_tag="IVZ67_002126"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8166191.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8857..9612)
                     /gene="fabI"
                     /locus_tag="IVZ67_002127"
     CDS             complement(8857..9612)
                     /gene="fabI"
                     /locus_tag="IVZ67_002127"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8166192.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9832..10272
                     /gene="fabZ"
                     /locus_tag="IVZ67_002128"
     CDS             9832..10272
                     /gene="fabZ"
                     /locus_tag="IVZ67_002128"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8166193.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10387..11130)
                     /locus_tag="IVZ67_002129"
     CDS             complement(10387..11130)
                     /locus_tag="IVZ67_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8166194.1"
                     /translation="MLSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASS
                     VDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGYYNG
                     LTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSN
                     GSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAI
                     ANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         3561 a         1962 c         2358 g         3249 t
ORIGIN      
        1 tctatggcaa tgattaaatt tgatcacgtg gataaatatt atggcaagtt ccacgcatta
       61 aaaaatatca atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg tccttctggt
      121 tcaggaaaga gcacgatgct tcgctgtatc aatggattag agacgatctc ttcaggtaag
      181 ctgctgatta atgatactga cttgcatgat aaaaagacaa aactgacaga agtccgaaaa
      241 aacatcggca tggttttcca acatttcaat ttgtacccaa ataaaactgt tctggaaaac
      301 atcaaattag caccaatcaa agtgttgaag caagaccagg caactgcagt caaaaacgcg
      361 gagaaatttt tgaaaactgt caatatgtta gataagaaag attcttatcc ttccatgtta
      421 tccgggggac aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg
      481 ctgctgtttg atgaacctac ttctgctttg gatccggaaa tgattggtga tgtattagac
      541 gttatgaaaa aattggctca tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc
      601 tttgccaaag aagtggcgga tcgtgttatc ttcatggctg atggagaagt attggaagac
      661 agcagaaatg tgagagactt tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc
      721 agtaaagtta tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct
      781 gattttcctt ttaggcattg cttttcttgc actagccgga tgtaaaggaa atgggttggc
      841 aaatgaagat attttagagc gaagcaaatc taccaatgaa atcatctggg gtgtcaagta
      901 tgatactcga ttatttggaa tgatggatat tgagagcaga actgtacaag gatttgatgt
      961 agatattgca aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt
     1021 tgaagtcact tctaaaactc ggattccatt attgaaaaac ggaaatatcg atgcaattat
     1081 tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt gacttttcag atgtctattt
     1141 cgatgctggc caagctttgt tggtaaagaa aggaagccaa atcaaaagtg tagatgattt
     1201 gaatgcttct acaacggttc tagcagtcaa aggttctaca tcggctgcga atatccgaca
     1261 gcatgcacca gatgcaaaaa ttttagaatt agaaaactat gcagaagcat ttactgcttt
     1321 acaatctggt cagggagacg cgatgactac ggataatgcg attttgttag ggattgcaga
     1381 tgaaaatcca gagtatgagt tagttggtgg aacgtttact aatgagcctt acgggattgc
     1441 gatcaacaaa gggcaagaaa acttcttgaa agctgttaac caagcgctag aggaaatgca
     1501 tgcagatggc acatacgata aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa
     1561 agtagagtaa gggggagagg caatgagcga attgatacaa acttatggac caatctttgt
     1621 cgacgggttc aagatcacat tgttttcgag tgtcttagca cttgtgttta gtttgattat
     1681 cggaacgtta atggcaatct tccaattatc aaagaataaa gtagtgagcg ccttagcaaa
     1741 ggcatatgta gaatttttta gaaacattcc attattaatc atcgtgatgt ttttttatgt
     1801 tgtcgtaccg atttattggt ttaatctaga tggattccaa gcaggtacga ttggattaac
     1861 gatctataca tctgctttta ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa
     1921 aggtcaaaca gaagcaggac tatcttctgg ttttacctat tcagaaatca tgcgttatat
     1981 tgttttaccg caagcattca aaatcgttat cccgccatta ggcaaccaat ttatcaacct
     2041 cgtaaaaaac tcatctattt tagcaatcgt agctggttta gatttgatgt atcaaggaga
     2101 cttgatcgct agtgaaacat tcaatacctt cgatacgtat atcatcgttg ggttatttta
     2161 cttagttatc acgctaccgt tatcttactt aatggtttat cttgagaaaa aatgggcggt
     2221 tcgttcatag aaggagggga aatacatgga ttttagcggc gctttttcat ggatgaatat
     2281 ccgtttttta ttggaaggat taaaggttac tgttgaagtt tcattgtttt cgatcatttt
     2341 tagtttcttg atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc
     2401 caaaatcctt ggtgtagtga ttgatattat acggaatctt ccattattat tgatcatttt
     2461 cttcacttac tttgccttac ctcaaatcgg cattcagatg aacatcttct ggtccgctgt
     2521 cgcagcatta acgatttttg agtcagctat gttgtcggaa attttccgag ctggactcaa
     2581 tgctgttcca aaaggacaga tggaagcagg attatcaacg ggactgactt atgtagaatc
     2641 gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca
     2701 attgatttca ctgatcaaag atacttcatt agcagtaatc atttctttac ctgaactttc
     2761 tcatcaggca cgtattattt atggacagaa caccaattat gtgcttccga tgttcatcat
     2821 catgacactt atgtacttcg tggtatgtta cgcgctttct ctgctttccg gttacttgga
     2881 aaagagacgg tacagttatt aatagatgat tcttttagcg gttgtggcaa actaaagtca
     2941 caaccgcttt tcctctttcc attcgaaaaa aagagatttc gattgtttta agcaagtaag
     3001 agcacttgaa aattcacgct aaaaagtaca caatagggag gatagaattt attgaaaaaa
     3061 aggatgatac gattgacaga taatcgccct atcggattta ttgattcagg tgtcggcggc
     3121 ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt atttgtagga
     3181 gacacagcac gctgcccata tggccctaga cccgcggaac aggtaatcca gtatacttgg
     3241 gaaatgacgg attatctggt ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc
     3301 gcaactgcgg tggctttaga agaaatcaaa gctgctcttt ctattccagt catcggtgtg
     3361 atccttcccg gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc
     3421 ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga gaaagtacca
     3481 gaattgactg tgacaagtct tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa
     3541 taccattcat cggtggcgaa aaaaattgtg gcagaaacat tagctccttt aaccactaaa
     3601 aaaatcgata cattgatttt gggatgcacc cattatccat tattacgccc catcattcaa
     3661 aatgtaatgg gagaaaatgt tcaactgatc gattctggag cagaaacagt aggtgaagta
     3721 tctatgctgt tagattattt caatttgagc aattcaccgc aaaatggtcg gacattatgc
     3781 cagttttata caactggctc tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga
     3841 atcggacact taaatgtaga acatatcgaa ttgggaggaa aataaggatg cgtcatgacg
     3901 ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac aaatgttttc aaatatccag
     3961 aaggatcggt agtgatccgt tttggtgata caacagttat ttgttctgca acaatagaaa
     4021 attctgttcc cccttttttg agagaaacag gaacaggatg ggtgacagca gaatatagta
     4081 tgctgcctcg agcaacgagt acgagaaatc gtagagaaag cagcaaaggg aaactttctg
     4141 gtagaacaat ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg
     4201 aaaaattagg agaacgtagt atcatcgtag actgcgatgt gatccaagcg gatggcggta
     4261 ctagaacagc aagtatcact ggggcatttg tggcattgaa attagccatt gaaaaacttt
     4321 taagagaaaa agaattgtca gaagatccaa taaaagaaca tcttgctgca gttagcgtag
     4381 gaatcttacc agatgggact tgcgtcacag atttggatta ccaagaagat tccgcagcac
     4441 ttgtagacat gaatcttgta atgacagaat ctggaaaatt cgtggagatt caaggaactg
     4501 gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg
     4561 cgattgaaga tttgatcaaa gagcaaaaac atgctttgct aacagagttt gctcaaaatg
     4621 atgagcggat agaagaaaca aaaacaatca tcattgcgac aagaaatcca ggaaaagcag
     4681 aagagtttcg aaatatgttc aaagaagcgg gatatcatgt aaaaacatta cttgactatc
     4741 ctgaattgcc agatgtggaa gaaactggca gcacatttga agaaaatgcc cgattaaaag
     4801 cagaaacgat tgcacaattg cttgatcagc cagtcttagc agatgattca ggtttgaaag
     4861 tggatgcttt aggcggtatg ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa
     4921 gtgatgcggg gaataacgcc aaattattat atgaattgac agatgtgccg gatgaaaaaa
     4981 gaacagcaca attccattgt acgttagtat tcgcggcacc caaaaaagac agccttgtag
     5041 tagaagcgga atggcccggt cgagtagccc gtataccatc tggcgaaaat ggctttgggt
     5101 atgatccttt atttatccca gaaggtaaga aacaaacagc agcagaatta tcgagcgaag
     5161 aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa attgagtgcc gagtggaaac
     5221 aatggctgga aggagagaga tagatgcggt atttagtagt aagtgataat catggagaca
     5281 gagatatcct cgttgactta gtagatcgtt atcgttctga agtggatatc tttttccatt
     5341 gtggtgattc ggaattagaa gcttctgacc cattatggga tacattccaa gtggttcaag
     5401 gtaattgtga ttatggacca ggattcgaac agaaaaaagt gatccaaact ggtcaggata
     5461 cagtattcat gactcacggt catttatcga atgtccgatt tggtttgaca cagttagcaa
     5521 tcgaagctcg agctgcaact gccgatatgg tgttttttgg tcatacccat caaataggct
     5581 gtgagatgga tcagaaaatc ctgtttttga atcctggtag catttctcag cctagaggcc
     5641 cgatccaaat cccatcatat gctcttatcg aaagtttaga tgaccaaatt tctgttcaat
     5701 actataaccg tgcccatcag gcagttgaag agttggcctt ttctttttcg agagagcgct
     5761 gaataaaaaa actctaattt ttaataaaaa taagctttat tttatgaatt ttgcatccgg
     5821 tttcggtaaa atagtagtgt tgaatgaaat agaaatggag gccatcagat gattggacct
     5881 atagtaagag aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg
     5941 gcaaatgtta tgtatcatca tccattgtcc catggtttac ttgtgttatc gaaggtcggt
     6001 tattccaaaa ttcctgtatt aggaaaagat gatcactttg taggactagt aagtttatca
     6061 gatgtggtca ataaaatgat tgacttgcaa acaatcagca tggacccgtt agaaggacta
     6121 acggttgctg atgtgacaga aacagatgtt ccgacaattg atgaaaattg ggaactagaa
     6181 gaagtactcc atttgttagt cgattcgcct tttttaccgg ttgtcacaga agatcggatt
     6241 ttcaaaggaa tcattacaag aaaagagctg cttaaagccg taaacttcat ggtgcatgaa
     6301 ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat taaaagaaca aatcgatatc
     6361 gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac
     6421 cagttttgac tttatcttga aaaagaggct gggacaaaag cgtttaactc caagaaataa
     6481 gaaggaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga
     6541 gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg ggacaaaagt gttctttact
     6601 tttgtcccac gctcttttta gtgagattcg ttgcatcgtt tgtccaaatt attttccgac
     6661 agcaattgga ctgttcggaa tcgtgaaacc agaagctgtc aattctttga cataggagct
     6721 caagaactct tcttttaagg catattgttt tccgttcaat gcgtagcaga ccgtacgaat
     6781 cgcaaaattg ctgtttccaa gatccactaa tccaaagata gaaggttcag tttggagctc
     6841 ttcttggtat ttttcagcta atgtacgatt tactcgatca atgatctcat agattttgtc
     6901 atatccttct tcaggaacaa tccgaatatc taaaagtact tgcatatttg cacgagagag
     6961 attgctgatc gtggtgatgt ttcgattcgg gatgaaatgg acagtaccat ccgttgcttt
     7021 cagctgaagg gtacggatac caacagatac gacggttcct tcaatcgtta agtttgctag
     7081 cttgatgtag tccccaacat ccatctgttg ttccatgata ataaaaaatc cggtaataac
     7141 atcactcatg aacccttggg caccaagacc gattgccaca cctgcaattc cggcaccagc
     7201 caaaagtgat ccgatcggca caccgatagc agataaaatt gcatagataa aaaagaaacc
     7261 aagggtataa tgaaaaatag tatttaaaag actatgtaaa gtctttaagc ggctctcgtt
     7321 tagaatggct tttttagaat actgattaaa agacttatca atcagatatt ttcccacacg
     7381 gtgaagaatc caaaaaagga cgatcaaaaa aatcagatat aaactttttt ggataaaggc
     7441 agccaaaatg gcgtcccaat caatcccgtc ccaaaaccgc tgaaacgcat ttactttgct
     7501 gacagcttga tctgtcaatg tctcgccaga agagtctgtc gtaccggcag ttgctaataa
     7561 tgaaaacata aaatcctctt ttctaaaatt tgattctttc tgtattgtac caagaagttt
     7621 tcaaaaaagg aaatagcaaa aataactata atgaaggaaa aaagctgaaa ataaaatgac
     7681 aaataatatg gataatgata aaatgtggag aaaataacga aggtggtaag tatatgcaaa
     7741 tcgattggaa caatctaggt ttttcttata tcaaaactcc ttggcgtttt attgccaaat
     7801 ggaaagacgg acaatgggaa gaaggagaat tgacagaaga taattacctg acgattcatg
     7861 aaggctctcc agcgcttcat tatggtcagc aatgttttga aggtctaaaa gcttatcgtc
     7921 gaaaagacgg caaggtcaat ctctttcgac ctgaacaaaa tagtcgacgg atgaatcaaa
     7981 gtgctagacg attgctgatg ccaatggtcc ctgaaaagat gttcgttgat gctgtcaagc
     8041 aagtggtaaa agcaaatgaa gattttgtgc cgccctatgg aacaggcgct acattatact
     8101 tgcgcccttt actgattggg gtcggtgaaa acattggtgt ccatccagct ccagaatatt
     8161 tatttgtcgt cttctgtacg ccagtaggag cgtatttcaa aggtggacta aaaccaacga
     8221 attttattgt atctgattac gatcgtgctg ctcctaaggg gactggtgca gcaaaagtcg
     8281 gcggaaatta cgcagcgagt ttacttccag gaacagaagc aaaagaactg gattactcag
     8341 attgtgttta tcttgatcca gccacacata cgaaaatcga agaagtaggt gcagcaaatt
     8401 tctttggaat tacaaaagac ggaaaattca tcactccgca atcgccctct atcttaccaa
     8461 gtatcacgaa atattccttg ttgcagttag ctgaagaacg actaggactg caagcaattg
     8521 aaggagatat tttcattgat gatctagatc aatttgcaga agctggtgca tgtggtacag
     8581 cggctgttat ttctccaatc ggtgggattt atcacaataa caatcttcat gtattttata
     8641 gtgaaacgga agtaggacca gtaacaaaac ggttgtatga tgaattgaca ggtattcaat
     8701 ttggagacat agaagcacca gaaggctgga ttgaagtcgt ggaataattt caaagtaatg
     8761 agttgaaaaa aacgtaaaaa gaaaaaatca ggaagagact ttaaaaagtt ttttcctgat
     8821 ttttatatgt tttactttaa agaatgcaac aaaagtttat gaaagatgga cacctttgtc
     8881 aacgtagata atatcaccga tgattccgct ggaaagtggg ctgattaaga aagcacaagc
     8941 attaccgact tcttcgatcg tcacaccttg ctgatcaggc gtacgagatt cagataactg
     9001 gatcagtttt tgatagtctt taacacctgt gacagctaat gttttgattg ccccagctga
     9061 gattccattg acccgaattc ctttaggcga gaactcagct gccaaataac ggatagcagc
     9121 ttcaagagaa gctttcgcaa tacccatcat gttataattc gggattgcgc gttctgaacc
     9181 tagataagtc atgctaacga tcccgctgtt tggtgcaaga aacggttgtg cgtattttga
     9241 tacagcaatt aaagagtagc tgctgatatc ttgagctaat tgatagccgt ctctggaaat
     9301 atcagacaca tttccggaca attcttcttt gttggcgtaa gcaactgcat ggacaaggcc
     9361 gtggatctcc ccagcatatt caccgatggc attcattgct ttttcgatgc tttcgtcgct
     9421 agaaacatca cattctacag tgaatgcttc ttcgccgacc aatttgacta atgatttttt
     9481 cattcgttca ttttggtaag tatagataat ttctgcccct tgtttcaaca ttgcttcagc
     9541 acaaccccaa gcaatgcttt ttttgttagc gacgcccata atgacgattt tttttccttc
     9601 tagaaatgac ataatgactc tcctttgttt ctgataaaaa tttgatagtc gtaaaaaaat
     9661 agagaataat agctaaaata acttcttgat ttcttaatta ttagtcaaaa tgttttgatt
     9721 gtcaaactat tttttcttaa attaaaaaaa gattgctaaa tatttaggaa tatgatactt
     9781 tgatagtcaa agtaatgaaa atggcaattg cctgaaagaa ggagtttaat tatgtcttac
     9841 gtattatctg ctactgaagt aatggaattg attcctaatc gttatcctat ttgttatatt
     9901 gattatgtag atactttaga accaggaaaa aagatcgttg cgacaaaaaa tgtaacaatc
     9961 aatgaagatt ttttccaagg tcactttcct gacaatccag tgatgccggg tgcgttgatt
    10021 ctagaaacac tggcacaagc aggttcgata cttatcctaa aatcagaaga gtttctagga
    10081 aaaagagctt atatcggtgg gatcaataag gccaaattcc gtcaaaaagt tgttccagga
    10141 gatgtgctga aatgtcattt tgaaatcacc aaaatgaagg gatcagtcgg tacagcgtta
    10201 tctgcagcat atgtagatga taaaaaagtc tgtgagtgcg agttcacgtt catcgtcaac
    10261 caaaatgctt gactataaaa cctgatatag caaaacaaaa ggaagcttac ttgcttttta
    10321 tgtaaaaaga agacgcctga atcctagcat acgttgtcat ctatgcagga ttcagacgtt
    10381 atttttttat ttttgttttg cttcttgcag tatattgatt ttatcgattt ttacttcttc
    10441 ttttggtttg tctgaactat ctgtttcgat atttgcaata gcatccacga catccattcc
    10501 ttcaatcact tggccgaaga cagtataatg gccatctagt gaaggataac cgccgttttt
    10561 ataagcgtcg atgattgctt gaggatagtc gtcttttaaa agaccatcat gcatatcatc
    10621 tgtattttga acgataaaga actggctgcc gttactgtta ggatcttgtg agcgagccat
    10681 agataacgca cctcttatat tataaaggtg gttgttgatc tctgtttcaa atccttctcc
    10741 ccaaatgctt tcccctccag tgccatcgcc tttaggatcg ccaccttgga tcatgaaatc
    10801 tttgatgacg cgatggaaag tcagtccgtt gtagtaaccg tcttttgcat gtgtcataaa
    10861 gttttcaacc gcttttggtg cgtgttcggg aaataatttg attttgattg tgcctttatt
    10921 cgtcaccatt tcgaccagat cttcattttc ggcgacatct ttcgtcaatt gtggtaattc
    10981 caatgcgttc aaatcgacgc tactggcagt cgtgctttcc gcagtagatg tactttttga
    11041 actttctgta gacgaactag aagaagtcgt gttgtttgca gaacaaccag cagcaaacag
    11101 caatgctgct gctaaactaa tacttaaaat
//
LOCUS       DACCZY010000095        11128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCZY010000095 DACCZY010000000
VERSION     DACCZY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.096"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="IVZ67_002130"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="IVZ67_002130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8166195.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="IVZ67_002131"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="IVZ67_002131"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8166196.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="IVZ67_002132"
     CDS             2139..3020
                     /locus_tag="IVZ67_002132"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8166197.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="IVZ67_002133"
     CDS             3017..3733
                     /locus_tag="IVZ67_002133"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8166198.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="IVZ67_002134"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="IVZ67_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8166199.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="IVZ67_002135"
     CDS             4450..5451
                     /locus_tag="IVZ67_002135"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8166200.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="IVZ67_002136"
     CDS             5448..6809
                     /locus_tag="IVZ67_002136"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8166201.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="IVZ67_002137"
     CDS             6769..7920
                     /locus_tag="IVZ67_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8166202.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="IVZ67_002138"
     CDS             7895..9064
                     /locus_tag="IVZ67_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8166203.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="IVZ67_002139"
     CDS             9239..10618
                     /locus_tag="IVZ67_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8166204.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="IVZ67_002140"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="IVZ67_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DACCZY010000096        11014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCZY010000096 DACCZY010000000
VERSION     DACCZY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.097"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            47..895
                     /locus_tag="IVZ67_002141"
     CDS             47..895
                     /locus_tag="IVZ67_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8166205.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1541..1747
                     /locus_tag="IVZ67_002142"
     CDS             1541..1747
                     /locus_tag="IVZ67_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8166206.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1949..2950)
                     /locus_tag="IVZ67_002143"
     CDS             complement(1949..2950)
                     /locus_tag="IVZ67_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8166207.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2955..4868)
                     /locus_tag="IVZ67_002144"
     CDS             complement(2955..4868)
                     /locus_tag="IVZ67_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8166208.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5036..5542)
                     /locus_tag="IVZ67_002145"
     CDS             complement(5036..5542)
                     /locus_tag="IVZ67_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8166209.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5702..6142
                     /locus_tag="IVZ67_002146"
     CDS             5702..6142
                     /locus_tag="IVZ67_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8166210.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6168..7325)
                     /locus_tag="IVZ67_002147"
     CDS             complement(6168..7325)
                     /locus_tag="IVZ67_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166211.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7328..7684)
                     /locus_tag="IVZ67_002148"
     CDS             complement(7328..7684)
                     /locus_tag="IVZ67_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166212.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7982..8956)
                     /gene="sgrA"
                     /locus_tag="IVZ67_002149"
     CDS             complement(7982..8956)
                     /gene="sgrA"
                     /locus_tag="IVZ67_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8166213.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9152..9991)
                     /locus_tag="IVZ67_002150"
     CDS             complement(9152..9991)
                     /locus_tag="IVZ67_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8166214.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10176..>11014
                     /locus_tag="IVZ67_002151"
     CDS             10176..>11014
                     /locus_tag="IVZ67_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8166215.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         3357 a         2167 c         1680 g         3810 t
ORIGIN      
        1 gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga gcgtctccat
       61 aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg ctatcgccga
      121 ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt tagcaagtga
      181 actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt ataatggatt
      241 taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta tttctatcca
      301 cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt tcgagtctag
      361 tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa aaaaagcggc
      421 tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt cagagattgt
      481 agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc acagcacgga
      541 ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg ctgttttgat
      601 ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa gaaaaactgg
      661 agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac ttggcgatgt
      721 cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag cttcccgtat
      781 ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca acgaaaaaga
      841 atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag aatagcacca
      901 taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata tttggtagtg
      961 gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt agaacgtatg
     1021 tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt agatgcgata
     1081 acaaagttag tatacagtag taaaaaattt atcttttctt ttttttatta tttttgtttc
     1141 ttattttcta aaaaattaaa aaaaattttg gtgaattaag tatttccttg ataacactgg
     1201 cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat atgattgtta
     1261 tgtaaaataa aaaagtttag gatacatcga atttttgggg aaattctttt gggggtatcc
     1321 taaatctttt ctattatatt cccatatttt ctattattgg tctagacgta tgatggtaaa
     1381 ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa
     1441 gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta
     1501 atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc
     1561 tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta
     1621 tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt
     1681 gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca
     1741 aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa
     1801 atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc
     1861 ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag
     1921 attacaacct cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat
     1981 atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat
     2041 ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta
     2101 cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg
     2161 tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt
     2221 ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac
     2281 ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct
     2341 gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg
     2401 ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg
     2461 accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga
     2521 taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt
     2581 gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta
     2641 catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg
     2701 tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct ttttttatat
     2761 cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac
     2821 ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca
     2881 aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact attcgcttga
     2941 accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca
     3001 gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac
     3061 attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta
     3121 tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc
     3181 ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt
     3241 atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg
     3301 aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc
     3361 tggtctttga gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg
     3421 aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt
     3481 gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca
     3541 tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat
     3601 ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc
     3661 tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc
     3721 ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg
     3781 tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg
     3841 acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca
     3901 ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg
     3961 acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct
     4021 ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta ttcttctgta
     4081 acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa
     4141 ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca
     4201 gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca
     4261 tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac
     4321 aatagataag tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca
     4381 gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta
     4441 agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca
     4501 cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag
     4561 gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta
     4621 gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt
     4681 tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct
     4741 acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg
     4801 cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag
     4861 ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac
     4921 aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat
     4981 aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt
     5041 gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct
     5101 ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt
     5161 atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac
     5221 ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa
     5281 atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca
     5341 atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa
     5401 ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt
     5461 ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta
     5521 ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat
     5581 ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg
     5641 gcacttttcg actatttctt catgttatta tttcataaat acttagctat tggaggaatg
     5701 tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg
     5761 gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa
     5821 aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac
     5881 tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact
     5941 atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc
     6001 tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa
     6061 aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa
     6121 taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa
     6181 tccaattttt ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc
     6241 agacgcgccc aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt
     6301 tgatcatatg cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca
     6361 gcggatgcac tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat
     6421 aattcctgtg ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt
     6481 ataaaaatat ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa
     6541 taagtattaa gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga
     6601 tacatcactg gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc
     6661 accttctttt ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat
     6721 agataatttt tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca
     6781 atagacgatt caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct
     6841 tcttccaaat aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga
     6901 gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta
     6961 aactcttctt ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta
     7021 atcgagtggc cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt
     7081 ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta
     7141 ttttttatac ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag
     7201 tgaaaaaagt atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg
     7261 caagataaat gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta
     7321 cgcaaactta tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa
     7381 gtgaatccag taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca
     7441 gtaaaaactg agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt
     7501 tatattctgg atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag
     7561 ccagggtgta gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt
     7621 cataacaata gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa
     7681 acaaagtaca tactcctttt tatctattat ttagacttat tccgacagct acatgaataa
     7741 cgtacaaata gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc
     7801 aattattctt gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt
     7861 tagtaaacaa aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga
     7921 atgaaatatt cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc
     7981 tttatttcac tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta
     8041 acacacccat ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc
     8101 tttttctatt atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat
     8161 caggctgttt tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg
     8221 gtatgatagg taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg
     8281 tgcttcctgt gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct
     8341 ccgtactgct ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac
     8401 tgctttcgct gctcgtgttt tctgtgctaa tttcactgct cgtactctct gtactgcttt
     8461 caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc
     8521 caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa
     8581 aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa
     8641 tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa
     8701 taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac
     8761 tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat
     8821 aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag
     8881 aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg
     8941 tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg ataaaggaat
     9001 tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt
     9061 cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca
     9121 agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca
     9181 tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg
     9241 tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt
     9301 ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag
     9361 ctccttgtgt ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac
     9421 tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg
     9481 tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta
     9541 ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg
     9601 atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc
     9661 ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc
     9721 caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac
     9781 cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa
     9841 cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca
     9901 tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaacaaag actattaaaa
     9961 cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa
    10021 caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat
    10081 agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct
    10141 aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac
    10201 tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca
    10261 atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc
    10321 caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg
    10381 atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa
    10441 tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga
    10501 catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa
    10561 aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac
    10621 agatcattct attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag
    10681 gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg
    10741 atggaaaaat gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag
    10801 catttaaact aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt
    10861 atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag
    10921 acaaatacaa atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat
    10981 tcatggataa aacaatccgt aaagtgatga aaaa
//
LOCUS       DACCZY010000097        10934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCZY010000097 DACCZY010000000
VERSION     DACCZY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.098"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            82..1041
                     /locus_tag="IVZ67_002152"
     CDS             82..1041
                     /locus_tag="IVZ67_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166216.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1306..1785
                     /locus_tag="IVZ67_002153"
     CDS             1306..1785
                     /locus_tag="IVZ67_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166217.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1818..4046
                     /locus_tag="IVZ67_002154"
     CDS             1818..4046
                     /locus_tag="IVZ67_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8166218.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4175..5818
                     /locus_tag="IVZ67_002155"
     CDS             4175..5818
                     /locus_tag="IVZ67_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8166219.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5891..6436)
                     /locus_tag="IVZ67_002156"
     CDS             complement(5891..6436)
                     /locus_tag="IVZ67_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8166220.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6459..6944)
                     /locus_tag="IVZ67_002157"
     CDS             complement(6459..6944)
                     /locus_tag="IVZ67_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8166221.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6934..7746)
                     /locus_tag="IVZ67_002158"
     CDS             complement(6934..7746)
                     /locus_tag="IVZ67_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8166222.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7885..9417)
                     /locus_tag="IVZ67_002159"
     CDS             complement(7885..9417)
                     /locus_tag="IVZ67_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8166223.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9526..10557)
                     /locus_tag="IVZ67_002160"
     CDS             complement(9526..10557)
                     /locus_tag="IVZ67_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8166224.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3663 a         2014 c         2095 g         3162 t
ORIGIN      
        1 aattaagcta gacaactaaa aaagtcattt gtgctacact caaaatagtt ccgaccaaag
       61 aattataagg agtgagtaca aatgacctat acccatctta caacggatga acttgtaata
      121 atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg cttgaatcgt
      181 tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc agccttagaa
      241 tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct tgttttaccc
      301 gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc cgatgtgatt
      361 gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg tatgttcaaa
      421 aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc gaatggacat
      481 caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga aaaggattat
      541 agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg tctgaaacat
      601 aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac attgaaaccg
      661 tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga atctgtaccg
      721 aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa ttggaaacag
      781 atcagtaatg tcaatgatat tgccatttat ttcgctgatc caggaacgcc gtctcaaaga
      841 ggcctaaacg agaattctaa cggattgtta cgtagagatg gtttattgaa atctatggat
      901 ttcaattcag tagatgaatt ttttattcaa tctgtcgcat ctaaacgaaa taatattcct
      961 agaaaatcac tggattatcg aacacctttg gaagtatttt tgagttacgt aagtattgat
     1021 gatctgtcta acttaatttg acaattaaga atacaaaaaa gatgttggtg aagttcacca
     1081 acatcaaaaa gtatagagcc aaaatattcc aacatcatct tggactttct acatgttact
     1141 aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt
     1201 tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac
     1261 taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca
     1321 aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat
     1381 gcattaaata tggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga
     1441 aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg
     1501 gaagaattac caatcacacg tattttagat ttagcgatct tagctgcgca aggaagttta
     1561 tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac
     1621 atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc
     1681 gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa
     1741 cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc
     1801 aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc
     1861 tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc
     1921 ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac
     1981 ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc
     2041 tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg
     2101 gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta
     2161 tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg
     2221 atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc
     2281 cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggct
     2341 atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta
     2401 actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc
     2461 cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg
     2521 aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca
     2581 tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg
     2641 cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag
     2701 gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt
     2761 cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg
     2821 aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa
     2881 aatggggtca tcctcaatct gagcatacaa aagaaagaac cattagcgcc gaagaagcaa
     2941 atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt
     3001 tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg
     3061 agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag
     3121 aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata
     3181 aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg
     3241 aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg
     3301 agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac
     3361 tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg
     3421 caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc
     3481 aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc
     3541 ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata
     3601 tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt
     3661 tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga
     3721 aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca
     3781 gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc
     3841 cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac
     3901 gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga
     3961 tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga
     4021 ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt
     4081 cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta
     4141 taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa
     4201 agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca
     4261 aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt
     4321 tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga
     4381 ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga
     4441 agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca
     4501 gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat
     4561 ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc
     4621 tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca
     4681 accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg
     4741 gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga
     4801 tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat
     4861 cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg
     4921 agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt
     4981 cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt
     5041 agatgtcctt aaattgaaac atgcattaag cgcttggcaa acttcattgg atggtatcgg
     5101 atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga
     5161 tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt
     5221 gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga
     5281 atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga
     5341 aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag
     5401 gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct
     5461 gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc
     5521 ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag gtctgatata
     5581 cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt
     5641 agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact
     5701 tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt
     5761 tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa
     5821 cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca
     5881 attactcatt tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg
     5941 ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgct
     6001 tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt
     6061 ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat
     6121 gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta
     6181 ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat
     6241 ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc
     6301 caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt
     6361 tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt
     6421 tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga
     6481 atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag
     6541 atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata
     6601 ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta
     6661 gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa
     6721 actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca
     6781 actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat
     6841 gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc
     6901 aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt
     6961 ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat
     7021 acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac
     7081 aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc
     7141 gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc
     7201 atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag
     7261 ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa
     7321 agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag
     7381 ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaacagggtc
     7441 taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc
     7501 acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac
     7561 taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc
     7621 tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt
     7681 taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct
     7741 tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag
     7801 ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa
     7861 atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg
     7921 aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac ggatgatagg
     7981 gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt
     8041 atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac
     8101 ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat
     8161 cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa
     8221 accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc
     8281 cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc
     8341 aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac
     8401 gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat
     8461 tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt
     8521 tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg
     8581 gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt
     8641 cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact
     8701 ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat
     8761 attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt
     8821 tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat
     8881 aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc
     8941 taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa taggaatctg
     9001 ttttgatttt gttccatttg aaatttctct cactcgcttt tttgtctcat catcaaataa
     9061 aatgaaaggg tcttctaaac gatcgataaa tacttccatt gttgttgttg gtagatgata
     9121 agaagtcatg acaggcactg caccaaggta agacacagaa acagctagaa ggtatgtgtc
     9181 aaaagcggaa ctcttataaa taataacttt gtctccggat ttcactccaa gatgagccaa
     9241 ttgataagcc cgtttcaaca cactttcgca gcattttttg taagttgttt caagtcctaa
     9301 ttctgggaaa gctgaaagtg gctcatcaaa gataatcggg gtctcggaaa agttagctgc
     9361 agcttcttga aaattcgtaa ataagttcaa aggggaatac gttatggtct ccatcattca
     9421 cactccatta tcttttgtta agtaatatac aaattcaaga taatcgtaac ataatccttt
     9481 atataataac atagtttatt gtgaaaaaaa gatcatacat ttgttttata tggaagaaac
     9541 ccgaacgaat gccgctaatt cgtgataaga aaaatcaacc atcttttccc agatatcttg
     9601 aaaagaatgg gatgagaggt atcgattacc acataaataa tttaccgcgt cagcatcatc
     9661 taaatatagt tctgcataat aacgatatgt ttttctgcat agttctggcg atacgttttt
     9721 atatactgta attttgctca aaatttggcg aatagtccga ggagaaattt gctgatcatg
     9781 tgaggaacga aaaacaaaag aattttcgtc cgtattttgt gctgttaact taaaatagtt
     9841 ctccagaaat ggcatcgctt cttttggtat tggtacttta ttcagtctgt tttcttttct
     9901 attcagatta agaatctgct tggtcaaaga tacatcttta cgtttaagtt ctactacttc
     9961 atggatcttt aatccgcaat acagaagaat ggcagtaatt gcaaggttcc tcggtctatt
    10021 cttttcttca aagctagctt ctctgcttgt ttgaaagcct tcaatcggga taaacaaatg
    10081 ctcaaaatct ttgaaaagtt ctgcatcgat atatcgcgga gctgctttgg ttgtttccgg
    10141 taaacttgta cccaaaagag aaatctcttc ttcaatcagt cctttttgat acaaaaatcg
    10201 taaaaaatga tttaatgaat attgcttcct tgaaatcccg taatagctat tttcaaacaa
    10261 ttgctccgtt tcttggttcg ctgtagtttg gaacgaacga gtatgtcgat agattttagc
    10321 taagaaattc tgataatctt gtttttctaa atggataatc agctgatcat ccacttcttc
    10381 agaaaatact tttctcaaat aatcagtaaa cagatgaaaa tcttttgcgt attcatagcg
    10441 actgcctaat gaaagggaag aacgattttc ttctaaaaac tgttgaaaga cggaagggag
    10501 caagtgtgta cgttgcttta acttttcttg cagttgtttt ctagttttgc tgtccattac
    10561 aaattccctc caatagtgtt cattttctat attttaccgc atttcatcaa atgaagatag
    10621 acagtttgtc ttgatcttag gaagcaaatt tttaactaag tactagctaa aagatatgaa
    10681 gtaagaagat acatttttag ttattttatt agtgcagtca ttcttttgat cttttaattg
    10741 atttatcctc tttttagcta tgtggttact tcatgagcaa tgaaatggat ttggttcgtt
    10801 ctaatggcag gaggctcttt cttttatttc cgaaaaatta gaaaacattt tttcagaatc
    10861 gattggctct ccatttattc aataatggtt tgtctaacga tattgttggg ataattctta
    10921 aagaagtatt agaa
//
LOCUS       DACCZY010000098        10888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCZY010000098 DACCZY010000000
VERSION     DACCZY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.099"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            12..84
                     /locus_tag="IVZ67_002161"
     tRNA            12..84
                     /locus_tag="IVZ67_002161"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IVZ67_002162"
     tRNA            88..160
                     /locus_tag="IVZ67_002162"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IVZ67_002163"
     tRNA            168..249
                     /locus_tag="IVZ67_002163"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IVZ67_002164"
     tRNA            267..339
                     /locus_tag="IVZ67_002164"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IVZ67_002165"
     tRNA            351..422
                     /locus_tag="IVZ67_002165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IVZ67_002166"
     tRNA            439..524
                     /locus_tag="IVZ67_002166"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IVZ67_002167"
     tRNA            535..608
                     /locus_tag="IVZ67_002167"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IVZ67_002168"
     tRNA            618..691
                     /locus_tag="IVZ67_002168"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IVZ67_002169"
     tRNA            711..783
                     /locus_tag="IVZ67_002169"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IVZ67_002170"
     tRNA            811..884
                     /locus_tag="IVZ67_002170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IVZ67_002171"
     tRNA            898..971
                     /locus_tag="IVZ67_002171"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IVZ67_002172"
     tRNA            995..1084
                     /locus_tag="IVZ67_002172"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IVZ67_002173"
     tRNA            1097..1170
                     /locus_tag="IVZ67_002173"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IVZ67_002174"
     tRNA            1189..1264
                     /locus_tag="IVZ67_002174"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IVZ67_002175"
     tRNA            1266..1336
                     /locus_tag="IVZ67_002175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IVZ67_002176"
     tRNA            1350..1423
                     /locus_tag="IVZ67_002176"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IVZ67_002177"
     tRNA            1448..1535
                     /locus_tag="IVZ67_002177"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IVZ67_002178"
     tRNA            1557..1628
                     /locus_tag="IVZ67_002178"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IVZ67_002179"
     tRNA            1651..1740
                     /locus_tag="IVZ67_002179"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IVZ67_002180"
     tRNA            1753..1826
                     /locus_tag="IVZ67_002180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IVZ67_002181"
     tRNA            1845..1917
                     /locus_tag="IVZ67_002181"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IVZ67_002182"
     tRNA            1921..1993
                     /locus_tag="IVZ67_002182"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IVZ67_002183"
     tRNA            1998..2068
                     /locus_tag="IVZ67_002183"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IVZ67_002184"
     tRNA            2081..2154
                     /locus_tag="IVZ67_002184"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IVZ67_002185"
     tRNA            2166..2237
                     /locus_tag="IVZ67_002185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IVZ67_002186"
     CDS             2503..3696
                     /locus_tag="IVZ67_002186"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8166225.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IVZ67_002187"
     CDS             3826..5298
                     /locus_tag="IVZ67_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166226.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IVZ67_002188"
     CDS             5381..6169
                     /locus_tag="IVZ67_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8166227.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IVZ67_002189"
     CDS             complement(6236..6904)
                     /locus_tag="IVZ67_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8166228.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IVZ67_002190"
     CDS             7019..8731
                     /locus_tag="IVZ67_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166229.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="IVZ67_002191"
     CDS             8734..10506
                     /locus_tag="IVZ67_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166230.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10888)
                     /locus_tag="IVZ67_002192"
     CDS             complement(10588..>10888)
                     /locus_tag="IVZ67_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP8166231.1"
                     /translation="IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHM
                     LRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3124 a         1962 c         2505 g         3297 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaatt
//
LOCUS       DACCZY010000099        10630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCZY010000099 DACCZY010000000
VERSION     DACCZY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.100"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..450)
                     /locus_tag="IVZ67_002193"
     CDS             complement(<1..450)
                     /locus_tag="IVZ67_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8166232.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVZ67_002194"
     CDS             674..1465
                     /locus_tag="IVZ67_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8166233.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVZ67_002195"
     CDS             1431..2384
                     /locus_tag="IVZ67_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP8166234.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVZ67_002196"
     CDS             complement(2782..3756)
                     /locus_tag="IVZ67_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8166235.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVZ67_002197"
     CDS             3871..4305
                     /locus_tag="IVZ67_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8166236.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVZ67_002198"
     CDS             complement(4430..5416)
                     /locus_tag="IVZ67_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP8166237.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IVZ67_002199"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IVZ67_002199"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP8166238.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IVZ67_002200"
     CDS             complement(6546..8468)
                     /locus_tag="IVZ67_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8166239.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IVZ67_002201"
     CDS             complement(8555..10495)
                     /locus_tag="IVZ67_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8166240.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
BASE COUNT         3253 a         2079 c         1820 g         3478 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg
//
LOCUS       DACCZY010000100        10492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCZY010000100 DACCZY010000000
VERSION     DACCZY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.101"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            11..634
                     /locus_tag="IVZ67_002202"
     CDS             11..634
                     /locus_tag="IVZ67_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166241.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1084..2133
                     /locus_tag="IVZ67_002203"
     CDS             1084..2133
                     /locus_tag="IVZ67_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8166242.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2130..3011
                     /locus_tag="IVZ67_002204"
     CDS             2130..3011
                     /locus_tag="IVZ67_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166243.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3014..3784
                     /locus_tag="IVZ67_002205"
     CDS             3014..3784
                     /locus_tag="IVZ67_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8166244.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4063..4917
                     /gene="aroE"
                     /locus_tag="IVZ67_002206"
     CDS             4063..4917
                     /gene="aroE"
                     /locus_tag="IVZ67_002206"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8166245.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5095..6258
                     /locus_tag="IVZ67_002207"
     CDS             5095..6258
                     /locus_tag="IVZ67_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8166246.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6375..6785
                     /locus_tag="IVZ67_002208"
     CDS             6375..6785
                     /locus_tag="IVZ67_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8166247.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6959..9187
                     /gene="topB"
                     /locus_tag="IVZ67_002209"
     CDS             6959..9187
                     /gene="topB"
                     /locus_tag="IVZ67_002209"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8166248.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9325..9642
                     /locus_tag="IVZ67_002210"
     CDS             9325..9642
                     /locus_tag="IVZ67_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166249.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9802..10038
                     /locus_tag="IVZ67_002211"
     CDS             9802..10038
                     /locus_tag="IVZ67_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8166250.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10035..10433
                     /locus_tag="IVZ67_002212"
     CDS             10035..10433
                     /locus_tag="IVZ67_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8166251.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3651 a         1767 c         2224 g         2850 t
ORIGIN      
        1 agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag aaatgaatga
       61 acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc ttgtttttct
      121 gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa aaatccgaga
      181 agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga aaccttttga
      241 ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga tgttcagcaa
      301 accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg agcttatgga
      361 agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg aactggaaca
      421 aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag gaaaagaagc
      481 aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt tgatccctga
      541 actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg atagaacggg
      601 agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga aacggtgggt
      661 ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa aataacaaat
      721 aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta aatcaattct
      781 taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa
      841 attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat agatacaact
      901 ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga ctgccgcata
      961 gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt
     1021 ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg caggaggaaa
     1081 aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta tttagcaggt
     1141 gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa
     1201 aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga tgagatttat
     1261 cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc
     1321 aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca acagctagta
     1381 caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc attagcaaac
     1441 acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg ggctggatta
     1501 gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca gccgcctgtt
     1561 acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg
     1621 tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga agctgcaaaa
     1681 aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg
     1741 gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa taccattgcc
     1801 aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt cattccatct
     1861 gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca atatcaatta
     1921 gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa accggaaacg
     1981 acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca agctgatcgc
     2041 ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac agatgactcg
     2101 cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca caaggcatgc
     2161 tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac tttcctgata
     2221 tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct attactcatg
     2281 gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata gcaggactcg
     2341 ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg attttagtga
     2401 tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt tctttattga
     2461 atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac tttgacacag
     2521 tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc ttcttcaata
     2581 cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct cggtcgttag
     2641 ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt gtcattgcat
     2701 tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa gggattggcg
     2761 taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa gaattgacgc
     2821 tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta ttgattttag
     2881 tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct atcatcttag
     2941 ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg ggttttgaga
     3001 aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa
     3061 tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca gagaagggga
     3121 cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca atacgatcgc
     3181 tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga tcacaaaaga
     3241 ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca agatgggaac
     3301 ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag gtcaaaaacg
     3361 gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac tatgtcaaga
     3421 agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat ctggtggtca
     3481 acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac tattattaga
     3541 tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat taacaaatca
     3601 gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg aagacgcctt
     3661 gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag atatttctga
     3721 aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag aagtagtgga
     3781 ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt gacatcttcc
     3841 tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg ttttgtagga
     3901 tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac aagtaaacga
     3961 tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt atcaaacgct
     4021 aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc tggacaaacc
     4081 aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt gatgcacaat
     4141 actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt aaatccaatg
     4201 aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt aaatctttcg
     4261 atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga agcgcaattg
     4321 atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta taataccgat
     4381 ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg
     4441 actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc gttagacggc
     4501 gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca
     4561 ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt tgccgatacg
     4621 catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac agatatcggg
     4681 atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca tgagaatcta
     4741 gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa
     4801 gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc aatcgcgttt
     4861 gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt tgattaaaaa
     4921 aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa gtaatccttt
     4981 atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg ataggctttt
     5041 gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac cacaatgagt
     5101 cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg ggatacgggg
     5161 agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga agatgctacc
     5221 cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga gcaacagata
     5281 tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt caatcggcaa
     5341 aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg
     5401 attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa atatgtagct
     5461 gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca aaaagcgacg
     5521 aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga tgccgggcga
     5581 gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa actgatttct
     5641 tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat caattcagct
     5701 tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt tggaatcgat
     5761 tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc
     5821 atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt agatattaaa
     5881 aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt ccgaatggat
     5941 gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc tattttcagc
     6001 caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa agctttagga
     6061 tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc gattgcacga
     6121 ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa
     6181 agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc tgctattaca
     6241 gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca
     6301 tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt taaccaagag
     6361 aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt gaagccatcg
     6421 ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac ggtgaaatgc
     6481 ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc
     6541 tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt ggtcctgaat
     6601 tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa gaaacgaagc
     6661 gcctatttga aagcttagct gagggtggta agattgagat gccactggat gctgttccgt
     6721 gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag attaaccaag
     6781 agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga tcatcgtttg
     6841 tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta ttgccagatg
     6901 cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag gtcagaagat
     6961 gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca
     7021 acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg ctggtgaagt
     7081 aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag cttatgaaga
     7141 gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct tccgttacga
     7201 agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc agtcagccga
     7261 taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat
     7321 tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga tcaattctct
     7381 tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga actattttcc
     7441 aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa tgaatggaag
     7501 tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt ctcttggtcg
     7561 tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca aacacttcaa
     7621 aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc agttttctgg
     7681 aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg aaatagaaaa
     7741 acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga aaaaggaaaa
     7801 gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga tgaatcagct
     7861 aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg aaagcaagtt
     7921 tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc agtatctggt
     7981 taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga caactgagat
     8041 caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg caattattcc
     8101 gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac agcaagcaat
     8161 ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata tctacgaaga
     8221 gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa agatcccatt
     8281 acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag ctcagactct
     8341 tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg agaaagaaac
     8401 acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa
     8461 aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga
     8521 agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg aagtcaataa
     8581 aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag ccaatgaacc
     8641 attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa
     8701 acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc tacatttatt
     8761 ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta gcgggacacg
     8821 tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa aatgtaaaaa
     8881 gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa
     8941 attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag atttgctttc
     9001 aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc
     9061 agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga tcgaaaacag
     9121 tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt
     9181 agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag aatataaata
     9241 ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg ctgattttat
     9301 atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa ttatcttatg
     9361 ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga ttacgggacg
     9421 aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat tttatcgact
     9481 ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag catgctaaag
     9541 cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat tatttatttt
     9601 ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga gagtttgtga
     9661 caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa gcaaaataaa
     9721 agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat agaatgagaa
     9781 tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc
     9841 aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt
     9901 gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa agcacaagaa
     9961 gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga cgcgttaaaa
    10021 gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag
    10081 ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat gcattagata
    10141 tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct gaaatctatg
    10201 aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat aatggcaata
    10261 aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt ccagttgctt
    10321 tttctcgtca agaagctgtt gattttatca taatgattac cactagttca ttggatttcg
    10381 attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa tgaacgaatt
    10441 aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga tt
//
LOCUS       DACCZY010000101        10470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCZY010000101 DACCZY010000000
VERSION     DACCZY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.102"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            124..342
                     /locus_tag="IVZ67_002213"
     CDS             124..342
                     /locus_tag="IVZ67_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166252.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(631..1143)
                     /locus_tag="IVZ67_002214"
     CDS             complement(631..1143)
                     /locus_tag="IVZ67_002214"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8166253.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1157..3454)
                     /gene="recJ"
                     /locus_tag="IVZ67_002215"
     CDS             complement(1157..3454)
                     /gene="recJ"
                     /locus_tag="IVZ67_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8166254.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3596..4030)
                     /locus_tag="IVZ67_002216"
     CDS             complement(3596..4030)
                     /locus_tag="IVZ67_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8166255.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4040..4828)
                     /locus_tag="IVZ67_002217"
     CDS             complement(4040..4828)
                     /locus_tag="IVZ67_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8166256.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4825..5766)
                     /locus_tag="IVZ67_002218"
     CDS             complement(4825..5766)
                     /locus_tag="IVZ67_002218"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8166257.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5943..6530)
                     /locus_tag="IVZ67_002219"
     CDS             complement(5943..6530)
                     /locus_tag="IVZ67_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8166258.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6751..8064)
                     /gene="obgE"
                     /locus_tag="IVZ67_002220"
     CDS             complement(6751..8064)
                     /gene="obgE"
                     /locus_tag="IVZ67_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8166259.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8352..9362
                     /gene="gap"
                     /locus_tag="IVZ67_002221"
     CDS             8352..9362
                     /gene="gap"
                     /locus_tag="IVZ67_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8166260.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9447..10382)
                     /locus_tag="IVZ67_002222"
     CDS             complement(9447..10382)
                     /locus_tag="IVZ67_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8166261.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2891 a         2365 c         1813 g         3401 t
ORIGIN      
        1 tcttttttcg cccgggttct tcttctttca aatttttatc acttccctct ttacaaaccg
       61 aacaaatatt cgtatactaa atatacgaac aattgttcgc ataaattaaa gaggtgagaa
      121 ttcatgaaat caatgagacg tcttggctta gtattaactg ttttgttttt aggtattatt
      181 attggtaatt tcggcatacg tccagcttta cttgtgtttg ttccgttatt tactttgtgg
      241 ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc ggcgcagaat tcatgaagat
      301 catttctatc atgagcccta ctacagatcc aggcaatcat aataaggatt gactgctaat
      361 tattcaaaag acaagaagtt aaacttttac gggggtgtga caaaaatcgg tcacatcctt
      421 tttttcgaat aaacggtgtt ctaaagccag tttttcggta ttaagtcaaa ttgcggaaaa
      481 agtgattttt taaacttcta ctggtctttg cttgtttcca tttataaaaa aatagcgacc
      541 ctcaaaagtc agatagagaa tctgactttt gagggtcgct ttaaaatcgc ttctttaaga
      601 ataacatcaa gaatagcttc ttttttctgc ttaatattcc attaatgtaa tgatgtcata
      661 tcctttgatt ttgtcgcgtc catggagatc catcaattcg atcaaaaatg cgcagcctac
      721 gactacaccg cctaattgtt cgatcagttc gatcgtcgct ttgattgttc ctccagtagc
      781 tagcaaatcg tcacaaatca gcacacgttg acctggttta atggcatcct tatgcaatgt
      841 caatgtatct gttccatatt ctaatccata agtcacttcg atggtttctc gaggtaactt
      901 cccttttttg cggactgggg caaaaccgac tcctaactca taagcaacag gacatcctac
      961 gatgaatcca cgggcttctg gcccaactac catatcaatt cgtttttctt ttgcataatt
     1021 tacgatttgt ttagtagctt cccgataagc gtcaccatct gccatcagtg gggaaatatc
     1081 tcgaaacaca attccttttg acggatagtc gggaatactg gcaatataat cttttaaatc
     1141 cactctttgt ttcctcctag gttgttaacc atttctttat ggtaggaata tcgctcaaaa
     1201 gcaaaaattc ctcaactttg attttattga gccgtcgctg gtataatctg ctctcagtca
     1261 aaggatgact ttctgggcta gcgacttttt gtaaaacacc gttctctatt gtaacaaatt
     1321 tcaattcaaa aaacacctgt atcataaaaa ttaataattt ttcagggata cgcaaatatt
     1381 gagctattgc ttttatttta tgacgtatat caattcgttc ttgagaatgg atcaatttaa
     1441 acaattttgc gtactgttct cttgtgccta ccccattcaa ataggcctca tcaggtgaaa
     1501 tccccatcag ataaacacga ttgaatgaat aagaccgaaa aacttctttc agatccgcta
     1561 aatcatctgg acaatccata acaactagag agtttggttt tcttttttca attattgcct
     1621 ttacagaatc tagtccttcg aaaacaatcg cagcttcctg gataatctca ggcacgaatt
     1681 tcaacgtcga agaatcaaat gctaaataaa ggctaccttc cgacgttcgg ctctgttcac
     1741 ggaaacgttt tgcccgccaa tcaaatactt gcagaccacc cacagaaaaa tcagatacca
     1801 tcagctgcgg tttcttacgc ccgttccatt cattgatcga aagctttcct accacatcta
     1861 cctgattatt cagcagttct tcttcttgag taccaaaacc aaaagcgacc gcatcaagct
     1921 gactcgatgc atcggaaagg acaaatttca gatgctgttg gtttgctccg attcttttga
     1981 tattttctgc ttggacttgt cgaaataaaa actgaggcaa aggattatcc gtaccaaatg
     2041 gcgccaatag tttcagctca tcaattcgct ctactgttac ttcatttggc agcaatactt
     2101 catcaattct caactcaggc cctctcatca agtcaatttg atgatcgact atatattggt
     2161 tcatcttctc ctgtagtata gtgacatttt cacttggcat cgttaaaccg actgctgcat
     2221 gatgtcctcc gaagtaagta aataaatctc gcatctggtc aagcatttca aaaagattca
     2281 aggcttcaat actacgtcca gatcctttag ctgatccatc ttcttttaaa gtaaggacaa
     2341 gcgttggttt acccgtttca ttcattattt tcccagcaac aatgcccaaa acgccttcat
     2401 gccatcctcg gtttaccaat agatggatat gattctcctt attgaccatc gctaatgctt
     2461 cttgtgttat ctgctctacg attgcttttc tttcttcatt gatctcattc aatttttttg
     2521 cttgaacgga tgcttcctct tcatcaaacg tcgcaagtaa cgagacggca ggacgtggat
     2581 cgcccatccg accgattgca tttagtctgg gagcaatcga aaaaccgatc gttgtttcgt
     2641 ctgcgtccct catttttacg ccgctttctt caaatagcgc ctgaagtcct atccgttctg
     2701 aatgatgaat ggcatcgatt cctaatgaaa caaggattcg attctcatca gtcaatgaaa
     2761 ctaaatcggc gatcgttcct atcgctacta aatccaaaaa ttctgcaggc ggttcttcca
     2821 ataatgcaga agctactttg aatgctactc cgactcctgc caaatctgga aatggatagt
     2881 gccccgctgg atgacgaggg tgaacaatcg cataagcgtc tggtaaattc tcaggtagtt
     2941 cgtgatgatc tgtaatgatc acgtctacac cagcttgctg agcgtatgct accgcatcat
     3001 ttcctgctac cccgttatca acagtgatga tcaactggat tccttcttcg atcttttctt
     3061 ggtataccga ttggtttggt ccatacccat gctcaaatcg atttggcagg aatacttcta
     3121 cttctgcacc aagtaattcc agtgtttctt tcattaccgt tgcactggtg atcccatctg
     3181 cgtcataatc accgtaaacg aggatttttt ctccactgat tacagcttct tggattcggc
     3241 tgattgcttt atccatatcg tgtatcagat acggatcatg taattcttgt tcctttggat
     3301 gtaaaaatcg gttgatatcc ttttcctcat ggtaacctct ttgccacagt aacttaccga
     3361 caaatggaga taatttcagt tcacggatca tttggagaaa ggattcagaa ggctctgtct
     3421 cgcttaactt ccaatcaaaa tttgcttgtc tcactttctc actcctaact tagttattat
     3481 agcaaaggaa atcagcaaaa aaaagcagaa agaaagaata aaaacagatc agatccgcta
     3541 gacagatgtt cagcaggacc tgatcttttg tacgaccatt tatgaaatat ccctattagt
     3601 caaaataatt gaagctttgt ttgggcgatt gagttttttg ttcttccggc gctttcaatt
     3661 gactgatttg tgcggttttc gcttgtaact ccgcctgata agcagcttcc aactccgcac
     3721 gttcgtttct aaactcgcgt tgctgttctt cttttgcttc ttcaattttc ttttgaagtt
     3781 ctgtttgata ttctgacatt tcctgagtca gctgtttgat ttgctttttt tgctgccata
     3841 gtgttgccga agcagtcaaa aatacaatca gtgcaccaat cagcgcagaa ccgatgatca
     3901 ctaagatcaa tggcgcagaa aaggaagtga agccaaaact tacaggaaca tttttattgt
     3961 tcaatacagc aaaaagaaca ataatcaaaa ctaaaacaaa tccaagaatc acattgcttt
     4021 gctttttcaa ttccttcacc tactttttgt taaaaattcc gctggctaaa taatcaccaa
     4081 tcgttggaaa cagcgtataa aattttgatg caacttctaa aataaacggt tgattgattt
     4141 cccgttttgg atgtctcata ttgcggacga tcatttttgc caacttggca ggttctaaaa
     4201 cgatttgtcc gactttttcc aaatagcttc cgcttggatc agccttatcg aagaaatttg
     4261 tctcgattgg acctggattc actgtcgtaa cagctacgcc aagtggtttc aattctaatc
     4321 gcaaagcgtt ggagaagcca aggactgcaa atttcgtggc agaataaacc gttgattttg
     4381 cagttgccat ttttccagcc atagaggcga tattaatgat atgaccttgt cttctttctg
     4441 ccatatcgat cgcaaatttt tgtgtcaaaa ccatcattcc taagatgttg acttcaaaca
     4501 tatcgtatgc ttttccaagg tcgaatgtaa ggaagtcttc gaaaatccca aaacccgcat
     4561 tgtttaccag aatatcgacg cgcccgactt cttccatcac cttttcatat aattcgtcca
     4621 cgctttcagg attgctgacg tctaccttga aagcataagc ctctttgccg ctcaagcgtc
     4681 tgcactcgtc tctgacacct tctacaaatg ccattcttcg tgcgcaactt ataacgattg
     4741 ctccttgctt tgctgcttca taacagattt gtgcgcccag accgcctgaa ctacctgtta
     4801 ctaaaacaac tttatcttct aaattcatgc ctcttcctcc tcgcgaaatg gaatctcaat
     4861 gctatcaaaa tctttcataa tcgtcgtgtt ttcaaacact tcttgtgctt ctttttcaag
     4921 ctctaaagct gcttttccta aatatctcgc actgatatga gtaagtagca gccgttttgc
     4981 ctgcgcttct tttgcaactt cagcagcttg atgagtcgtg gaatggaaat agttgtgcgc
     5041 cattcttgct tcatctttat taaatgtgct ttcatgtacc agaatatcgc tattttccgc
     5101 aagcacaacg gaattatgtg tttttctcgt atcgcctaaa atagtcacga ttcgtccttt
     5161 tttatctgga cctacgaaat cttttccatt gatcgtccgc ccatcttcta gttggatcgt
     5221 ttcaccttgc ttcagtttgc cgtacagtgg tccagctgga atacctaatt cttttagacg
     5281 atccacctgc aattctcctt tatgatcatg ttcaaccaca cgataaccaa aactgtcgat
     5341 cccatgattt agtttttttg tataaacaga aaattgctga tcagtaaaaa tcggggctgt
     5401 ttcatttagc tcaatgaatt tcagcggata acttaaacgc gtttgcgaaa tacctaaaga
     5461 gacttttatg tattcctcta tcccttttgg cccgtaaatc tctaatggcg tatccccacc
     5521 ttgaaatgac cggctgctga tgagcccggg aagtccaaaa atatgatcgc cgtgtaaatg
     5581 tgtaatgaaa attttaccga tttttctcgg acgaatagtc gttcgcaaaa tctgcatttg
     5641 cgtgccttcg ccacagtcga acaaccaaac ttcattgcgc tcgtctaaaa gtttcaatgc
     5701 tatacttgtc acatttctat gttttgcagg aacacctgca cctgttccta aaaattgtaa
     5761 ttccataatc tacctgactt tctttaagtt cctctttaat tttagggtaa aaagaaggga
     5821 taatcaaccg taagtcgaga atgggttcaa attaaaaata acgttctaaa gcgatagcgt
     5881 tttctttgaa aattactata aaaacagcag cgaacccgtt tgagatcctc tgctgttttt
     5941 tattaatttg aagatccaga actttcattg tcgttggaag tttgcggatg tttgtccttc
     6001 caagtctttt gtgcagcttc tttcgcatga ttttcagctt ctttcgcatc atgcacatct
     6061 ttttcgacat cttcataatc caagcgagta atttctccgc ctaattccat cattaccaat
     6121 tgattcagtg ggcgattgtc ctcttcatca gcaatcaaga tcagtcctgt agctccttca
     6181 ccgattttat ttcctacaaa gtcgaacacg gtctgggcat cttttatttc tttggcatct
     6241 tgtgtcgcac cgatcattcc tccggcaaac catcctaata aaatccctag cggaccacct
     6301 aatacaccaa ccaacattcc gattaatcca cccgtagaag atttgttatt accggtaaag
     6361 tcgataaaat cttctatttt gaattgatgt gtcccattag gatcatggct gacaactgcc
     6421 atctgttctc ctttaatcga acgaacagca tgtaactttt tgatttctga aaatgcctga
     6481 tatgcctggc tttctacttg aaaatttaaa atgattactc gtttgctcat taaaaacgcc
     6541 cctttcatac cttcttgtta tttattttac ctttaattga aaaataagaa aagaaaagca
     6601 ctcttttttt ccaataaaat agggctgtga caaaagtcgt atcacagctc ctttttccga
     6661 ataaacggcg ttcttgaagc tgatcacttc tgtcacaacc tcccttaaat ctgaatctat
     6721 attttttggt atttgttttt atttgcttgc ctattcgaca aattcaaatt caaactcacc
     6781 gatacgtaca aggtcgccat ctttcgcacc gcgtgcccga agagcttcat ccacacccat
     6841 accacgtaat tgacgagcaa aacgcatcac tgtttcatca tgatcgaaat tcgtcatttg
     6901 gaataatttt tcgatcttct caccagataa gatccacgtt gcatctgaat cacgatcgat
     6961 ttggaactca ggaccttctg gattgaatcc atagtgtaca gtttcttctt ccagttcttc
     7021 ttcgtacaat gggaattctg gcgttacttc taacaaatct gctgtagcat tcaataacgc
     7081 gtccaatcct tgtcgagtca ctccagaaat tggaaatact gggatgtcat cagcaaattc
     7141 atcttctttt tccttgttca gctgttcttt gaatttcact agattttccg gtgcatccgg
     7201 catatccatt ttgtttgcca cgatgatttg cggacgctct agcaagcgta agttataagt
     7261 agataattct ttgttgatgg caagatagtc ttcataagga tctcgtcctt ccatcccact
     7321 catatcgatc acatgtaaaa ttactcgtgt tctttcgata tgacgaagga attgtgtacc
     7381 cagcccaaca ccttgcgaag ctccttcaat caagcctggc aagtctgctg cggcaaaact
     7441 tctgccatca cttgtagtaa ccattcctag attaggtacg agcgtcgtga aatgataagc
     7501 gccgattttt ggacgagcag atgaaataac ggatagtaaa gtcgatttcc cgacagatgg
     7561 aaaacctaca agtccaacgt ctgccaatac tttcaattct agttcgattt ttcgctcttg
     7621 tcctggttca ccgttttcag caatttccgg tgcaggattc cgaggagagg caaaacggat
     7681 attccctcgt ccaccgcgtc caccttttgc cacaactagt gtttgaccat tttcaattaa
     7741 atctcctaaa agagcaccag tctccgcatc tcggacagtg gttccttgtg gtactttaac
     7801 ataggtatct tctgaaccac gaccgtgcat ccctttactc atcccatttt cacctggctg
     7861 cgctttgaaa tggcgattga accgaaaatc catcaatgta cgtaaccctt catcgacgat
     7921 caagatgaca tcgccgccac gaccaccgtc tccgccagcc ggtccgccgt caggaacgta
     7981 tttttcgcga cgaaatgcaa ccattccgtc tccgcccttg ccagctttca catcgatcgt
     8041 tacttgatct aaaaacatgg acataattaa gtcctcctat tattcattta tttatttgac
     8101 tgcctgaatt tccacaataa acaaaagtct tacttatttt atcggtgaaa tctgattttg
     8161 tctatcattt cagcaaaaag attgtttacc ttcttctttt tttatcattt ggtaaggttt
     8221 ccaacctttt ttctgctacc aagtataatt ttttgacaaa atattctttg cgtagcagaa
     8281 taacagtgta ttaattgtat taattgagga tgccgtttca cagcacccct aataaaggag
     8341 gaacttttga tatgacagta aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg
     8401 ctttccgccg catcaaagaa gtctcagatg atattgaagt agtagcaatc aacgacttaa
     8461 caagtccaac tatgttagca caattattgc aatttgactc tactcatggt acttatcctg
     8521 gcacagttac tgcaacagaa gactcaatcg tagtggatgg cgaagcaact cgtgtttatg
     8581 cagaacccga cgcaagcaaa atcccttggg caaaagaaaa cggtgtagat atcgtactag
     8641 aatgtactgg tttttataca tctgaagaaa aagcaaaagc gcatttagat gctggtgtca
     8701 aacgagtagt tatttctgcc ccagctggtg ctatgaaaac catcgtctat aacgtcaatg
     8761 atgatacatt agatgaaaac gacaaaatca tctcagcagg ttcatgtaca acaaactgtt
     8821 tagcacctat ggcttatttc ttaaataatg aatttgggat cgaagttggt acaatgacaa
     8881 cagttcatgc ctacacttcc acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt
     8941 tgcgtgctgc tcgttcagct gctgacaata ctatcccaca ttctactggt gctgcgaaag
     9001 caatcggctt agttatccct gagctacaag gaaaattaca aggacacgca caacgtgttc
     9061 cagtcgttga tggttcatta actgaattgg tttcagtatt gaaaacaaaa gtaactgctg
     9121 atcaagtaaa cgaagcaatc aaaaaacaca cagttgacaa tccttcattc gggtatgatg
     9181 accgtcaaat cgtttctagc gatgtcattg gaacaactca aggttcgatc tttgacccaa
     9241 cgcaaacaga agtaacaact gctggtgatt tccaacttgt taaaactgtt gcatggtatg
     9301 acaatgagta tggatttact tgccaaatga ttcgcttact agaaaaattc gctaacttgt
     9361 aatttgatag atcaatcgat aaaagaagcc tttgactgga tccagtcaaa ggcttctttc
     9421 ttttcatccc ctacttttaa actcgttcaa tgaacatatt ggcttaaaaa ttgttggact
     9481 acttgctgat atttttcagg atctgtcttg tatgcttccg catgctcggc tccttttaca
     9541 acatactttt cttttggtcc cttcgttgca cgatagacct tatctaacat ttcaaatggt
     9601 acaaatgtat cttcgtctcc atgtataaag agcattgggc gctggttttt cttcaattgt
     9661 tccactgcat ttgcttcacc gaaaaagtag ccagcacgga cttttgtcac tagactcgtt
     9721 acttgcataa gcggaaatgc tggaagacta aacatatcgt ctagctgata agcaagctca
     9781 tcatgaacgg aagaataacc gcaatcttca atgattgctt tcacattatc tggtagtttt
     9841 tctccgctag tcatcataac tgttgctgct cccatgctga caccgtatag aacgatttct
     9901 tgcgattttc cattgttttc tagcactttg tttatccact gtacatagtc tttacgctct
     9961 ggccatccaa atccaatgta atccccttcg ctttttccat gtcctcttgc atcaggaaca
    10021 aggacattgt aacccatatc atgaaacatc tttgcgtaat tggtcatcgt ttcagcgttt
    10081 cccatatagc catgagccac aagagctgtt ttttcacttt tcgtttcagc aggaagataa
    10141 atcgctgata atttcagacc gtcttttgaa gtgatggtcc aatcgcttcg gttttctttt
    10201 gaactaaacc atttatgtgc atccgtttct ttggatgtct gtgttgtacc aggtgtatct
    10261 tcgtctaaaa agtctttctc agaaggcaca acagcataat tataaaaata attccctgca
    10321 aacactaacg caacaatcag agctaaagcg acgcctaaaa ggattttcat ccaaagtttc
    10381 ataaatcatc catctcattt ctcttttttc atataacctt gaattagtat aatcaaagaa
    10441 tctctactat gcaatgttca tttttataac
//
LOCUS       DACCZY010000102        10252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCZY010000102 DACCZY010000000
VERSION     DACCZY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.103"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            93..2789
                     /locus_tag="IVZ67_002223"
     CDS             93..2789
                     /locus_tag="IVZ67_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8166262.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2866..3354
                     /locus_tag="IVZ67_002224"
     CDS             2866..3354
                     /locus_tag="IVZ67_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8166263.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3366..3860
                     /locus_tag="IVZ67_002225"
     CDS             3366..3860
                     /locus_tag="IVZ67_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166264.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3881..4690
                     /locus_tag="IVZ67_002226"
     CDS             3881..4690
                     /locus_tag="IVZ67_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166265.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4687..5508
                     /locus_tag="IVZ67_002227"
     CDS             4687..5508
                     /locus_tag="IVZ67_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8166266.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5559..7658
                     /locus_tag="IVZ67_002228"
     CDS             5559..7658
                     /locus_tag="IVZ67_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8166267.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7655..8773
                     /locus_tag="IVZ67_002229"
     CDS             7655..8773
                     /locus_tag="IVZ67_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8166268.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8812..9690
                     /locus_tag="IVZ67_002230"
     CDS             8812..9690
                     /locus_tag="IVZ67_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8166269.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9881..10033
                     /locus_tag="IVZ67_002231"
     CDS             9881..10033
                     /locus_tag="IVZ67_002231"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166270.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3155 a         1679 c         2288 g         3130 t
ORIGIN      
        1 aaatcatatt agataaaaac taaattggta gataaattat tttgtcagat atctgaggca
       61 aatattcgac tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat
      121 tgcagccgat agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga
      181 gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca
      241 ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga
      301 cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc
      361 ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg
      421 tagttggtca tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat
      481 atccgcctaa cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt
      541 tagccacaaa aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat
      601 ttgctatctt aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt
      661 ttggatacaa gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa
      721 cggccaacaa cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag
      781 aaaagctctt tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt
      841 ggcattctgc taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat
      901 tagacacctt tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc
      961 ccattcgaga acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac
     1021 aaaaagatat tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg
     1081 ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa
     1141 cacaagaaga accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg
     1201 aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa
     1261 aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga
     1321 taaatgaagg taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg
     1381 tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt
     1441 ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg
     1501 ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg
     1561 ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct
     1621 tggagcattt accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg
     1681 tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg
     1741 gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt
     1801 ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga
     1861 atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt
     1921 taaatcaaat ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata
     1981 atttgacgac ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta
     2041 aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct
     2101 tttctcaaag tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat
     2161 tgaaagatgt ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact
     2221 acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat
     2281 tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg
     2341 acatcttaga cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc
     2401 cggcagaatt taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg
     2461 cggaccgttt gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt
     2521 ctaaatatga aaattactat catttcactt taaatggccg tgtaaaacta aatctctata
     2581 tgcatacggc attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac
     2641 cgaattatga tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc
     2701 aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg
     2761 aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt
     2821 ttaggttaga cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt
     2881 ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa
     2941 attctaatag cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg
     3001 acggggaagg ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa
     3061 caggagttga atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc
     3121 gatttatttg gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt
     3181 gctgtaacta ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc
     3241 aaggaattaa caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag
     3301 atgaccattc ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa
     3361 gaaaaatgat taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga
     3421 cttggacaaa gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca
     3481 acgatattca aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta
     3541 aaacagttga tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga
     3601 tggttttagt ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa
     3661 tcgaattaat gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa
     3721 ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg
     3781 aactttatcc agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct
     3841 ctattttgag tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt
     3901 cgctttggcc gtttttattg gagttgctgg acatgaaatg ttcgggatgt ccatgctgag
     3961 tcgtccgatt gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt
     4021 gattgtcggg gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag
     4081 tacggcggag ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg
     4141 aaacgtggat gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat
     4201 gaatatgatt ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa
     4261 atctggtgat caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc
     4321 gctttatgct cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt
     4381 cttggataat attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc
     4441 ggcagttggg atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt
     4501 tttctttggg gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt
     4561 tggaacgatt attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt
     4621 tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt
     4681 ctttcaatga aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg
     4741 ttttggcgct cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt
     4801 ttcgcctatt ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct
     4861 gcggcgctgg aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc
     4921 atggggattg cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa
     4981 agtatcaacg cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg
     5041 ttctggggag tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga
     5101 aatattttgg caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga
     5161 tactatggcg gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg
     5221 aacgggttaa ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt
     5281 gcaatgacga tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa
     5341 gaactaatga atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg
     5401 gtaacattga gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt
     5461 ggaatggtcg tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca
     5521 gctactaggc tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt
     5581 attcacccaa acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa
     5641 acgaatacag ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta
     5701 atcaaaagac attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc
     5761 gagaaaattt actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa
     5821 ccaattcgtt atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg
     5881 atcatcgtat tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt
     5941 cgacgtttaa ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta
     6001 tgcttgttca acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga
     6061 aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa
     6121 aaggaaataa ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg
     6181 acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga
     6241 aggaaacatt gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag
     6301 ttgtccatct ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac
     6361 agggacaggt cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata
     6421 aggatatgta ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa
     6481 cggttctatg gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta
     6541 tcaatcattc tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac
     6601 atactgggga tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa
     6661 ttattgagga catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt
     6721 ggttatcaga cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga
     6781 aggcctgtat tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc
     6841 gatacaaaaa cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg
     6901 gtccttttgg tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag
     6961 tgaagcacta tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg
     7021 tggatggaaa aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc
     7081 ctttttatca atttttagat aataaagatc atttatatca gaacaccatg gccttttctg
     7141 ctagttcttt caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat
     7201 ccggcgccac ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag
     7261 ctttcgtaac ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt
     7321 ggtggccgta tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca
     7381 aatgcggctg ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga
     7441 aattcaaaaa aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga
     7501 atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt
     7561 caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa
     7621 agggaaaaac gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca
     7681 atttaaaaaa atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa
     7741 agctgcgatg ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct
     7801 gacttctggt gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat
     7861 gtggatgacg attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt
     7921 aagaatttat tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt
     7981 ttgtaatggg tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac
     8041 agggggcttt aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga
     8101 gaatttacat ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga
     8161 cgaattggaa gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa
     8221 attacaggag gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac
     8281 ttaccttgcg cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta
     8341 cattgatggg gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg
     8401 cggggcgtgg gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc
     8461 aacagccttt ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga
     8521 aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct
     8581 ttatcgttgg catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca
     8641 acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc
     8701 ttactggtat tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg
     8761 cttgccgcgt taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat
     8821 ggggcaattg aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg
     8881 attttggaaa gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt
     8941 gattttttta aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca
     9001 attgatattc aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct
     9061 tggcaaaatt tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg
     9121 acaacagatg tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta
     9181 gcaggtgttg catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg
     9241 actttcgtgg aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca
     9301 aaggacaatt tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca
     9361 ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg
     9421 ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt
     9481 tcgccagata gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa
     9541 gttcaggata aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc
     9601 tacattatta cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc
     9661 agaaaagcgg taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca
     9721 aaagtattta accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc
     9781 aaataaaaag gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct
     9841 tctgcgtttc cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg
     9901 cgtacactta aatagtaata tattgtgtta ctttatgtct gtggtaatag ttatagttgc
     9961 ttaccaatgt atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga
    10021 atttttttgg tgatagattt tttcagccgt ataaaataaa tcgtaattct attatagaga
    10081 gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt ttctatacat tttaaatatt
    10141 ttattcctat ttctattgct aaaaaagtag aaattctata tttaccttta actctcgctt
    10201 ctcttgcaaa tgtagttagc attttaatcg ataataaact caggtagtta ta
//
LOCUS       DACCZY010000103        10023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCZY010000103 DACCZY010000000
VERSION     DACCZY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.105"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..506)
                     /locus_tag="IVZ67_002232"
     CDS             complement(<1..506)
                     /locus_tag="IVZ67_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8166271.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="IVZ67_002233"
     CDS             779..3250
                     /locus_tag="IVZ67_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8166272.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="IVZ67_002234"
     CDS             complement(3353..5089)
                     /locus_tag="IVZ67_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8166273.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="IVZ67_002235"
     CDS             complement(5086..5790)
                     /locus_tag="IVZ67_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166274.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="IVZ67_002236"
     CDS             5921..6424
                     /locus_tag="IVZ67_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8166275.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="IVZ67_002237"
     CDS             6384..6722
                     /locus_tag="IVZ67_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8166276.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="IVZ67_002238"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="IVZ67_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8166277.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="IVZ67_002239"
     CDS             8273..8851
                     /locus_tag="IVZ67_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8166278.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="IVZ67_002240"
     CDS             8855..9376
                     /locus_tag="IVZ67_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8166279.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="IVZ67_002241"
     CDS             complement(9455..10009)
                     /locus_tag="IVZ67_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8166280.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3218 a         1862 c         2076 g         2867 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tcc
//
LOCUS       DACCZY010000104         9957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCZY010000104 DACCZY010000000
VERSION     DACCZY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.106"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            140..3367
                     /gene="fss3"
                     /locus_tag="IVZ67_002242"
     CDS             140..3367
                     /gene="fss3"
                     /locus_tag="IVZ67_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8166281.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3480..3794
                     /locus_tag="IVZ67_002243"
     CDS             3480..3794
                     /locus_tag="IVZ67_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8166282.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3807..4181
                     /locus_tag="IVZ67_002244"
     CDS             3807..4181
                     /locus_tag="IVZ67_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8166283.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4191..4526
                     /locus_tag="IVZ67_002245"
     CDS             4191..4526
                     /locus_tag="IVZ67_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8166284.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4609..5949
                     /locus_tag="IVZ67_002246"
     CDS             4609..5949
                     /locus_tag="IVZ67_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8166285.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6027..6701
                     /locus_tag="IVZ67_002247"
     CDS             6027..6701
                     /locus_tag="IVZ67_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8166286.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6934..7368
                     /gene="dcm"
                     /locus_tag="IVZ67_002248"
     CDS             6934..7368
                     /gene="dcm"
                     /locus_tag="IVZ67_002248"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8166287.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7369..8076
                     /locus_tag="IVZ67_002249"
     CDS             7369..8076
                     /locus_tag="IVZ67_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8166288.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8066..8356
                     /locus_tag="IVZ67_002250"
     CDS             8066..8356
                     /locus_tag="IVZ67_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166289.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8615..9799
                     /locus_tag="IVZ67_002251"
     CDS             8615..9799
                     /locus_tag="IVZ67_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8166290.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9814..9933
                     /locus_tag="IVZ67_002252"
     CDS             9814..9933
                     /locus_tag="IVZ67_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8166291.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3296 a         1679 c         2248 g         2734 t
ORIGIN      
        1 ttttagatta aatttttagg ctcattcatt ctcaaatcca ttctattcag tcaataaaaa
       61 ttttaaaaag actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcttaa
      121 tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat
      181 tttgagtttg gctgttcaag caattgttgg agcaattgct cctacgatta cttttgctga
      241 tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt
      301 tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt
      361 gtacaacggc tttaaacaag atattttctg tattgaacca ggagtcccga tttacaatga
      421 gttcactcct ggctatgaga aaaacccgtt accagatatg tccgataaag ccaaattagt
      481 ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat
      541 gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc
      601 cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc attgacgatt atcagaagaa
      661 accaaccttc cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga
      721 tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg caaacattga
      781 ttatcgtgtg aatggtaatc agttgataat caccccaaat gccaattcaa aagaaagtgg
      841 tgtgctgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg
      901 ccaacagact ttaatggccg gggcgattga taaacctaat acctacacaa tcaaaattga
      961 tgtggaaacg gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt
     1021 accaggaacg gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac
     1081 tgacaaagaa ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac
     1141 cgaaaaatca gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa
     1201 agcaggcgaa accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct
     1261 ggataaaagc ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg
     1321 taatacgttt gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac
     1381 ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac
     1441 ctattatgtg accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa
     1501 agtcgaattg aagtatgcca atcaaaccgt agcccctgtt accagtaacg taaaagggca
     1561 aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa
     1621 agcacaaggt aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg
     1681 aaaagctgtt aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc
     1741 tgatgaaacg gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc
     1801 tatcaacgag tattattggc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac
     1861 aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg
     1921 aaatgttacg gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg
     1981 atcagctgcg ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt
     2041 ggaagggacc aatgaaatca caggtgccga agatgaagcg accacagctt ataacgagca
     2101 attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga
     2161 agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt
     2221 taaggaaaac aaagaagact ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga
     2281 ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aagctgacga acaaagcatt
     2341 ttctgttagt ttgaaccgtt taatgctcta tgatttgcct gaggaagaag atagtttaac
     2401 ttctcttgcg acttggaaag acggaaacaa agaattaact tctctggatt ctaccgagct
     2461 agttgataaa ttgagttaca atctacatga aatcaaagaa gactggtatg tggtagcgca
     2521 agccattgat gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt
     2581 ggtgattgca gaaacaagcg caacgttggc gaacaaagag aaaacaggaa cttggaaaat
     2641 tcagcataaa ttaaccgccg aacaagtttt aaataaaact attgtcttat tcaactatgt
     2701 gtatgaaaat aaggaagctt ttgaagcagg cgataagcca gtagcgaagg atgttagctt
     2761 gaataaccaa gcccaaactg tcagttgtac agtggaacac catgtttcta tccaaacaaa
     2821 ggcccaccta gaaaacggtt ctcagacctt tactcatggt gatgtggtgg atatgtttga
     2881 tgatgtgtcg attactcatg atgtactgga tggctcaaaa gaagctttcg aaacaattct
     2941 gtatgcttta ttaccggatg gtacaaacaa agaaatttgg aaatctggca agattgatta
     3001 tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa
     3061 atatccagaa ggaaccaagt ttacttttgc ggaaatcaat tacgataaag atgggaatat
     3121 caatggaaaa cacaatgaag atttgaaaga aaaatctcaa accttaacac caaaagaagt
     3181 tccaaccata ctaagtacgc cgaaacaacc ggaaacacca actgttccaa gtggctctca
     3241 agaatctagt cccacagtga agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt
     3301 actgttcatt ggttttactt tgatttttgc gacggcgggc tattatttct ggaatcgccg
     3361 gaactaaggt gatgagatga tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc
     3421 taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga
     3481 tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta agttatgccg
     3541 gtgaaggaga aattataaca gaaggatatg gacggaatac tacggtgatt ggtcgcagtt
     3601 atcatttgta ttccagtaag caacgggccg atgatattga ggtggtagta gctgcggaag
     3661 ccggtgaaaa ggattttgat caagaccaac cgctaaaagc cgtgaatccc catttggttg
     3721 ccaaaggcta tgagattgag aatcgtgggt ttactgatta cgtgttgtat gttgatgatt
     3781 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc gtagattcag
     3841 gtttaacatt tgggaagttg cgtttttccg cattacgccg agaagtacgg aagcaaaatg
     3901 aggatggaac aattggtaat gaggtaaaag aacgaaccta taatttaaaa tcctctgcac
     3961 aagggcgaat gattcaagtt agtatcccag ctaatgtacc cttacgcgag tttgcttatg
     4021 atgcagaggt agagttggtc aatccaatcg tggataccgt tgccaattat gtttttcgag
     4081 aaggaactac cgttaattgg ttcattaagg cggatgattt agtcttgaag cggcagccga
     4141 atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt atggaaatta
     4201 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggcgagctg actgaggaac
     4261 aactcgttaa agcattaacc tctgatgaaa aaatgtttct gattatacat caaggtttac
     4321 ttggagaaaa tagcgacagt gaagaagact ctgatgtact agactggtta ggagaagaag
     4381 agtctttctt tatagttgtc gaagtaaatg aaaacttatg tcggcaggca gaatccattt
     4441 tggaagaaat tggtgtggag atgccggatg ctattgaagg ctttttaaat caattggttg
     4501 aaacgaaaca actcccctcc tcgtagcagt aaatgactag cataactttt tgaaattgcc
     4561 taatgaaatc ctattgggct ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga
     4621 catcgcatac gcgcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc
     4681 gctctgttca tcggttggat gagcgtcttt tattttcaag agtttagaca atttgatatt
     4741 tctaagctat cactttctac tattgaaatc gtttggtcta taaaagattt agtatgtctg
     4801 cttggaagtc tcgctttttc aggagctatg atattgcttt atattcactt ttttcttgat
     4861 cattggcgaa gcttgtggca tcgacaaaag ctagctcgga tgattctaga gaaccattgg
     4921 tatgaagtga aacaaactca aagtgaaggc tttttcaaag atttgaatag tagccgaacc
     4981 agagagacta tcagttactt ccccaaaatc tgttatcgaa tgaaagatgg attactgtct
     5041 attcgcgttc aaatttcact tgggaaatac caagatcagc ttttaaaatt ggaaaagaag
     5101 ttagaaagcg gactgtattg tgagttagta gaaaaggaac tcaaagactc ctatgtggag
     5161 tatactttac tgtatgatat gatcgccaat cgaattggga tagacgaagt agtggcagaa
     5221 aatgggactc tgcgattgat gaaaaatcaa gtatgggctt atgattcttt accccatatg
     5281 ttaatcgctg gtggcacggg tggagggaag acctatttcc tcctcaccat tatcgaagca
     5341 ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcaga tttagctgat
     5401 ttaggtacgg tgatgcctca tgtttattca caaaaggaag aaatttctgc ttgtgtggaa
     5461 gatttctatg aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc taactacaaa
     5521 acaggagaga attatgccta tcttggactt ccacccaact ttttaatctt tgatgaatac
     5581 gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa
     5641 caaatcgtga tgttgggtcg tcaatctggt ttctttctga ttttggcttg ccaaaggccg
     5701 gatgcgaaat atttaggaga cggaatccgc gatcaattca actttcgcgt ggctttgggt
     5761 cgtatgagtg aactcggata ttccatgatg tttggggagg tcgaaaaaaa cttctttatg
     5821 aagcacatca aaggtcgagg ttatgtggat acaggtggca gtgtgattag tgagttttat
     5881 acgccacttg taccaaaagg gtatgatttc ttagattcaa ttagtaaatt agaaaagatg
     5941 aatgattgag tgatcttcaa tactgacttt atacttcaag taacgtataa tacttttata
     6001 tagtaggaag aaaggggata attaatatgg aaagtaatga ttcttattta gagtatgcaa
     6061 aagcaaataa ggataagttt atttcttata ttatcaaagg gaaatccccc gaagttggac
     6121 gagacgcagt ttttatggca gggagtcctg gggctggaaa atcagaggtt gccattggat
     6181 tagctgagaa ttatgataac catgtgatta ttgatgcgga ttactttcgc acccagtttc
     6241 ctgattataa tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg ttggtagagc
     6301 aggcatttag atatgtctta gatcatggct attcttttat tctggatgca acttttgcaa
     6361 tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga gtgacgattt
     6421 tctatgttta tcaagaccct aaaatcgctt ggaaatttac acaagaacgt gaaattgcag
     6481 agggacgtca tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca agagagaaca
     6541 ttgcaaaagt aaagagtaaa catccagatg tattgcttca tatcataata aaagattata
     6601 cgaataatat ttccgaagtt cattatgcta cggataatct agaattattt ttaccactag
     6661 ggtatagtag gaaagatttg gaggttcaat tacatgactg agaaagaaca agtaacgaaa
     6721 attgtaaaga aatacaataa gagcattgcg gatttatcgg aaaatgctac tgcaaaagag
     6781 ttcaaaacgg tgattaaata tgtagccgat caggccaatg aaaaacaacg gaaattagtt
     6841 ggattaaata aaaaatagta ggacaaatta ataatgtatg agccaagagc aaaatgttct
     6901 tggctttttt ctttgcaatg aatgaggtaa tgtatgagat ttttagattt attcgccggt
     6961 attggtggct ttcgtttagg tatggaacag gcgagccatc attgtatcgg tttttgtgaa
     7021 attgataaat ttgctagacg tagttataaa gcaattcatg atacaagtaa ggaggtggaa
     7081 atgcatgaca tcacaagtgt atcagatgag tttattcaat ccctcggacc agtggacatc
     7141 ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aagattttcc
     7201 gatactcgag gtactttatt ctttgaaatc gctcggttca ccactcttct ccgacctaag
     7261 cttttattcc ttgagaacgt ccgaggattg ctcaatcacg aaggaggggc tacgttcgag
     7321 acgatcctcc gaacgctgga tggattgggg tatgacgtgg agtggtaggt gcttaactca
     7381 aaggcctaca ttccccaaag ccgggagcgg atattcctta tcggacattc tcgagacgca
     7441 tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa catctgatca aaacatcaga
     7501 aatttggtaa atatcaatcc ttccaatcga ggaatgggtg gacaagtcta tggttcagat
     7561 ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa tcgcactgcc agcaagggat
     7621 ggaatctctg tagcaggact attaccggga aagtttgaac aaggtaatcg agtttatgaa
     7681 gttactggaa ccgcccccac cttgtcaacg aaacaaggtg gtacaaaaat catgattcga
     7741 aaaaatgaaa cagaatatca agaggtgaag ccagaagaca gtgtgaatct ggcttttcct
     7801 aattccacaa gtcgccgagg tcgtctggga aaacagagtg ttcatacctt actaacagga
     7861 gaccaacaag cgatcgtcac ggatcagtat caaattcgga agctaacacc tagagagtgt
     7921 tggcgacttc agggctttcc cgattgggct tttgatcgag attctcaagt caattcagat
     7981 agtcagttgt ataagcaagc cggaaactct gtaacagttc cggtcatttt tgatattgcc
     8041 agaagattaa aagagttgga tgtacatggt ttatgatgag ttgattggag aaatttattg
     8101 ggtgattgga aagattcagt cagacccaga attagaagaa caattgcgac ggttaaaatt
     8161 tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca tatttaatga atgaggagac
     8221 ggatgctcga attgaaataa atcaagtaat tgcagaattt gagcggattg cggaccaagc
     8281 aaaagaacca gatattcgtc aatacttgtt agaaatgaaa gcagagttgg aagtaaatgg
     8341 tattacagat gagtaaccta aaccaacaca actgactgtt aggcgctgac cgcggaggat
     8401 tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcgtt agccgaccat
     8461 gatgtctcac ccccgtatct aacagggggg tacaaaaaca caaaaaataa gtaagcaacc
     8521 attaagaatg cttgttgtat caaagatagt aagtaatttg tataggtgtt aactcatttg
     8581 aaaaagttaa caccttgaat ttggaggtga gaacttggca caacggaatt tagattatcg
     8641 gctattaaaa gaccgacgca acgaatacgg cgtttcacaa aataaattgg ctacggcttg
     8701 tggacttagt cggccgtact taaatcagat agaaaatggt ggcgtaaccg catctataaa
     8761 aaccatgagg aaaatttttg accaactgga aagctttaat cccgatttgc ctttgacgtt
     8821 actgtttgac tatgtaagga ttcgctttcc cacaacggat gcacgaaaaa ttattcaaga
     8881 aattctccat ttgaaatttg agtatatgct ccatgaagat tatgcctttt actcctatca
     8941 agaacagtat gtcatgggtg atattgtggt gatgttgtct catgaagaag ataaaggtgt
     9001 gcttttagaa ttaaaggggc gaggttgccg acaatttgaa acttttttac tcgctcaaaa
     9061 gcgtagctgg tacgactttt tcgaggattg tctaaaggcc ggtggcgtga tgaaacgctt
     9121 ggacttggca atcaatgatc gagtggggct attggatata cctgagctaa cgaaaaaatg
     9181 tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa agttatcgtt ccggggaact
     9241 tttaaaggct gatgaaaagg acggcatggg caatacctta tatatcggca gtctcaagag
     9301 tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa tatatcaagt tagggattcc
     9361 attagaccaa actgagacga aaaatcgttt tgagattcga ctgaaaaatg accgtgcttt
     9421 tcatgcgatt caagatttgt taaaaggtcg tagcattgag agcaccacgt tttccattat
     9481 caatcgttac ttgcgatttg cggataaagt agaaggtaaa aggcgaacca actggccctt
     9541 gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa gagattcaat taacttccga
     9601 gccaaaaccg tataccattg aacgaacctt gaattggcta ggccgacaag tcgcccctac
     9661 gtggaaaatg gctaaggagt tggatcgatt gaatcggaca acctatatcc aggatatggt
     9721 acagaatgca cgcttatcag accgacataa aaagattttg gagcaacaaa gtatggcaat
     9781 tgaaaatttg attatatgaa aagaggaatc gtcatgggaa ttgtaaaaga tatttttttg
     9841 gtatttggtg gcagtatgct tggtgttact gtgatgtgct tgatgaatgc aagtgcagca
     9901 gcggatcgag caatggaaaa gaaaggaaag tgacaagatg aatttcggac aaaattt
//
LOCUS       DACCZY010000105         9895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCZY010000105 DACCZY010000000
VERSION     DACCZY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.107"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(116..2236)
                     /locus_tag="IVZ67_002253"
     CDS             complement(116..2236)
                     /locus_tag="IVZ67_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8166292.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2466..3644
                     /locus_tag="IVZ67_002254"
     CDS             2466..3644
                     /locus_tag="IVZ67_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8166293.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3660..4418
                     /gene="aroD"
                     /locus_tag="IVZ67_002255"
     CDS             3660..4418
                     /gene="aroD"
                     /locus_tag="IVZ67_002255"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8166294.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4441..4926
                     /gene="ybaK"
                     /locus_tag="IVZ67_002256"
     CDS             4441..4926
                     /gene="ybaK"
                     /locus_tag="IVZ67_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8166295.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4997..6250)
                     /locus_tag="IVZ67_002257"
     CDS             complement(4997..6250)
                     /locus_tag="IVZ67_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8166296.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6367..7716
                     /gene="gdhA"
                     /locus_tag="IVZ67_002258"
     CDS             6367..7716
                     /gene="gdhA"
                     /locus_tag="IVZ67_002258"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8166297.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7828..9174
                     /locus_tag="IVZ67_002259"
     CDS             7828..9174
                     /locus_tag="IVZ67_002259"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8166298.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9482..9868)
                     /locus_tag="IVZ67_002260"
     CDS             complement(9482..9868)
                     /locus_tag="IVZ67_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8166299.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3156 a         1800 c         2024 g         2915 t
ORIGIN      
        1 aaaaaatctg agccagaatg aggttcattc tgacccagat ttgattttgt acaacccatc
       61 agtccttatt gacaaagagc ctaattttgt cgctgctcct tttaaattta ttttatcatt
      121 cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa
      181 cagttgattg tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt
      241 cagcttcttt taatttctct aagccattct tgtagtcata atcactggta tcaattgggt
      301 ctaatccact attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag
      361 ctaactgttt atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag
      421 gttcagtaat agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt
      481 ttggtgtaac aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata
      541 gatcttgtcc taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag
      601 gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga
      661 tatggatcat ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac
      721 ttgtttttcc aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt
      781 caccgtaaag gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga
      841 attcttgtac ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat
      901 catttgtatt ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat
      961 ggttagatac agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta
     1021 cggattgttg gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa
     1081 catcataata agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc
     1141 gattccagaa tgtaccagcg ttcccatgga ataccgcact agtgtaacct tgtgtttgac
     1201 ttaaaatatc aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc
     1261 cttgattcaa gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg
     1321 cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat
     1381 tgatgaaagg tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca
     1441 ggaattgctg gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga
     1501 acaaatcgtc atttggtgct gcataatgtt cttttacata attttctact tctttcatat
     1561 cattgggact tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa
     1621 ccatgaaagc attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca
     1681 gcaattctgg acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta
     1741 atgtagagat cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt
     1801 ttgcaatcag cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc
     1861 gaaacagtcg aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg
     1921 tgttgatcgt aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca
     1981 atgtcaaaag aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat
     2041 aaagagcaat tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt
     2101 attgaatgcc actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca
     2161 accaaagtaa aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg
     2221 tttttataca tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga
     2281 aaccatgtta cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca
     2341 attgaaacca gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg
     2401 agcagtaaga caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg
     2461 aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac
     2521 tgatccaaaa agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag
     2581 ttgattcgca aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg
     2641 gttgggtatt gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg
     2701 tgaagtcaaa agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta
     2761 tattcaatgg agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg
     2821 ctgttttttc ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt
     2881 ttcgtgaaat ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa
     2941 atctgcctga gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag
     3001 aaaacggagt aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag
     3061 accaaaaaga agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga
     3121 acatgtttag ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa
     3181 caacaagtgt cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga
     3241 atggtttgga tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat
     3301 acgccaaaag aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca
     3361 gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc
     3421 tggatatttt agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga
     3481 tagaacaatt ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac
     3541 aacttgcttt atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca
     3601 attatctgaa agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa
     3661 tggttcgggt aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta
     3721 tcggggccgt ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg
     3781 atttagtcga atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag
     3841 aaacattggt tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta
     3901 ccttagctga aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac
     3961 atgtgataca cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc
     4021 taggaaaaag actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc
     4081 atgattttaa agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac
     4141 attttggagc cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc
     4201 ggttgatgga gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt
     4261 ccatgggaaa tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga
     4321 catttggttc cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat
     4381 tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt
     4441 ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt
     4501 ccctatacag agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct
     4561 gaacagcttc cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg
     4621 acggggcctc ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca
     4681 aaagtttcag ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc
     4741 ggatacatac gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta
     4801 gatcaaattg cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag
     4861 atggagttag cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa
     4921 caataaatca tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt
     4981 ctttcttttt tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag
     5041 cgaaagccgc tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg
     5101 caaaatacag accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag
     5161 aaacccctcc gccagttaca aatgatttct caataggaaa agttttgttg attgatagct
     5221 taaactcttt gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg
     5281 tcagatgatc catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac
     5341 catgccaagc attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa
     5401 taagctcttg tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg
     5461 ttttctctaa ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac
     5521 cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta
     5581 aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg
     5641 gttcatccga tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc
     5701 gcttcacgat cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag
     5761 ttgttaagat aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt
     5821 tatgttcttt gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt
     5881 ctttcagacg attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca
     5941 tccgtccatg atcctcttct tttaaatgta cgccttgtga ttcaaagaag ttcatgatat
     6001 cgaaattatt ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg
     6061 caatcaaatc atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca
     6121 gtagtttttt tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag
     6181 ctgcactgat cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca
     6241 gtttttccat tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa
     6301 aaatgttttg agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt
     6361 ggaacgatga caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag
     6421 gatcaagggc aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct
     6481 tttttggaca ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca
     6541 gaaagagtta ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat
     6601 agagggtacc gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc
     6661 catcctagtg tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat
     6721 agtttaaccg gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga
     6781 aaatctgata atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat
     6841 atcggcccat cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc
     6901 tatttgttcg gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa
     6961 cctttggatt tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat
     7021 tatgtcaaac atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttatt
     7081 tccggaagcg ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa
     7141 gttgttacat gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa
     7201 ctattgaaag aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt
     7261 ccttctgcta tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc
     7321 gctttgccat gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa
     7381 aatgggatca aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct
     7441 gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta
     7501 gcagtatctg ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa
     7561 gtggatgagc agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca
     7621 gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa
     7681 gttgccaaag caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat
     7741 tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt
     7801 agagtaaacc tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta
     7861 gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac
     7921 aacgaattac gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca
     7981 acgaactatg acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct
     8041 gattctgaag tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg
     8101 atcgacttct tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca
     8161 caaatcttct ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta
     8221 atcggtgatc gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca
     8281 gcgattgcgt tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca
     8341 aacaaacgta tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat
     8401 gcagaaggtt gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta
     8461 actgctgttg gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa
     8521 ggtgccgctg atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac
     8581 caatacgcag caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc
     8641 aactatgaac caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct
     8701 gaaggaaaag accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac
     8761 tctttaggac aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt
     8821 gaaaaacctc gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc
     8881 tacttgcaag gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt
     8941 gctcatacag acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat
     9001 acattgggtt atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat
     9061 ggaatcaacc cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt
     9121 ctaggcaaac caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag
     9181 ggtttttcgc tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact
     9241 ggtacccatt taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca
     9301 agacgtttgt cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt
     9361 tgttttagga ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt
     9421 tctagaatca gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag
     9481 cttaagatag gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt
     9541 ttttctctat gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag
     9601 cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac
     9661 cgttttcagc ataagcatct acgttgtatt taaaacctct tccaattact tccccattat
     9721 ctgctttgat atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac
     9781 ctacataagt agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga
     9841 taacgatcaa ccctaaaatt tttttcatga aagatctcct tttttatttt tatta
//
LOCUS       DACCZY010000106         9745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCZY010000106 DACCZY010000000
VERSION     DACCZY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.109"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            43..3321
                     /locus_tag="IVZ67_002261"
     CDS             43..3321
                     /locus_tag="IVZ67_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8166300.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3862..5769)
                     /locus_tag="IVZ67_002262"
     CDS             complement(3862..5769)
                     /locus_tag="IVZ67_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8166301.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5899..6774
                     /locus_tag="IVZ67_002263"
     CDS             5899..6774
                     /locus_tag="IVZ67_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8166302.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6854..7828
                     /locus_tag="IVZ67_002264"
     CDS             6854..7828
                     /locus_tag="IVZ67_002264"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8166303.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8017..9153
                     /locus_tag="IVZ67_002265"
     CDS             8017..9153
                     /locus_tag="IVZ67_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8166304.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9290..9634
                     /locus_tag="IVZ67_002266"
     CDS             9290..9634
                     /locus_tag="IVZ67_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8166305.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3172 a         1802 c         2059 g         2712 t
ORIGIN      
        1 ctttaagtaa acgctttcta atttgttacg ggaggaatac acttgttaaa aaaacgtata
       61 aaatacagtg cgctaatgat gaatctcctt atgctcagta ccccggtttt aatttctgtc
      121 gttgaagttg ctgctaacga acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt
      181 gaagggatgt caacaatgga ggaaatacca agaactagtg aagatatgga tacggaagaa
      241 ggaaatgatc ctatgatgaa agaatcagaa aatgaaaaga caaaaaagca atctgaagaa
      301 gcaaatgaag agtggaaagg ggttgttttc ggagaaagca caagtgctag taggtatgcc
      361 attgaaccat tagaagacgg cgtgcggctc tcttccacga ataacggcgg aaaattccag
      421 tcaagcggat cagatggaat gacctattat tatcaagcaa tccccaaaga catcaacttt
      481 aggatgcggg cgacgatcga aatagaatct tggacctata cgaatgccca agaaggattt
      541 gctttaatgg taagagacgc agtaccaaaa aatcatttgt ttgggcaggc attttattca
      601 aattcatttg ccatactagg cagtcggatc gaatatgtct gggactcgga tagacaagaa
      661 gtggtcaaca cgagcggcag caaatatacg atgcgacttg gattggggac acgggcaatc
      721 acaggaatat catctgaaga tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa
      781 acaacacctt tagagacttc agcagggttc ttggaaaaag aaacaggttc ttataatata
      841 ttcggaaacg gtcatgggaa tctgtttccg gccacgaact cgattactaa actggacgta
      901 gagatgaaaa aaacgaatac tggtttagag gcatattatt acgatcctga aacaggagaa
      961 gaaatggctt ctgacatcat gtatgactgg gaaaaattat acgcttcaga tgaatcggtt
     1021 atttatgcag gatttgctgc agcaaggaat atgacgatca aggtagaagg aatcgaagtt
     1081 gcttactcag atcctgcgac agatcctcct gggcaagaaa gacctactcg cttgattgat
     1141 ccgatttaca ctgtgacctc tagtaatcat tcaggaaacc aagatcatac tatgtccttt
     1201 agagcaaatg ctgacggatt attgacaatt aaaaataaag caacaggaga ggtgcttaag
     1261 aacgcaaaaa acttggctat cactgcgaat cgggaatttg attatcagac aaaactgatg
     1321 gaaggaacga acgaatttac tttcagtttt acgccagata aaaactatcc gccagaagaa
     1381 tacgtagaga tgactagtta tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa
     1441 cctaaatatt cgacagttta tgtaacacca gaagggtcag atgcaggaaa cggaagcaga
     1501 caaaatccat tatctctgac acaagcattg agatacgcat atgctgggca gacaatcgtc
     1561 atggcgcctg gaacttattc cttcgaaaga ggattgacga ttccaaaagg cgttaacggg
     1621 acaagagaaa acccgattca gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta
     1681 cttgatttta aaggaactgg aaatgggatg acactgttca gtcactactg gggtttacga
     1741 gggtttgaca ttacaaatag tgcagctatg caaaaaggat tacagatttc tggaaatcat
     1801 aatttgatcg aagagattca tgcttatcgg aatggaaata caggaatcca aatcagcggt
     1861 agctcaaatg atcctttcga agcatggccc gcgcataact tagtcaagaa ctgtacatct
     1921 tttgaaaatg ccgatccagg atttgaagat gccgatggat ttgcggccaa actgacaata
     1981 ggagaaggaa atgtatttga cggttgtatc gcgtatcata atgcagatga cggttgggac
     2041 ctttttgcta aaaatgaaac aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct
     2101 tatcgaaacg gctgggtacc aggaattgag ggagaaggta acggtaatgg attcaaaatg
     2161 ggcggttctt ctttaacggg ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt
     2221 gcgaaaggaa tcgattccaa tagcggaaca gatattatcg tcaatagcag tacaagtttt
     2281 aataacggtt catataatgt ggctctttat acctcttcag caggaaacac agactaccat
     2341 gcttctggta tcttatcttt ccgaacagag aatcttgaga tgagagagca gatccgccca
     2401 ttgaatcaaa acgaagatca aatctaccac tcgttgaact actattggaa tgaacgagag
     2461 aaacaatcag aaaatactct tggacatacc atatcaaaag attggatcga gtctctaaat
     2521 acatgttcga aagaaggtca gcagcctgtt acgcgctatg aaaatggaag tatccatgtt
     2581 catggactga tgcagattaa accggggaat cgtcaaacag aaaaggaaag agtggatggt
     2641 cttccattat ctgtaggtgc tatttttgac ggagaaacct caccgtcttc ggaatatcca
     2701 gagtatgcaa tcgtacaaga accaaatcag acaaaaccaa atgaacaaaa taatccgatt
     2761 attatacaag aagcaaatgg tgtggcggag aatgcagaag gaaatatcat tgaactgaag
     2821 ccttttacag tttttgtgtc aactttcaca ccaaatcaag gaacgcctgt ctttgaagat
     2881 atgcgttatt tcccagaaaa gatccaaatc gttgaaacac agtcaagtgc tcaattttct
     2941 aatcataccg ctattttttc agcaccttca aaaggagaat atacgctaga agtcacttat
     3001 cgttcggaaa tatttgatgg ggaaaaatgg tctgctcata cagaatctgt tacccagcaa
     3061 aaagcgatca ctgtgcgaga aactgccgat gtgcccccgg gaaatgacaa accagaaaga
     3121 gaaaatccag gaaatgaatc accaaatgga aaaccaaatg gaaaaccttc tcatccggat
     3181 aggacagcaa aagaaagtga aaaaaatggc actcaaggac tcccaaaaac gggagaattt
     3241 aataacccgt tgctagcatt agctggagga attttgttaa tcggtgtagt tgtttatgtc
     3301 atgaaacagc gaaaaaaata agtggaattt ctccttttat gatgtaaaac aattgactga
     3361 ggaaagtaaa aatagaagga aatgagacca atagatgtct cacttccttc tatcttttta
     3421 tagatgaggt tctccttgct gcttttgatt ggctgttctt attacttgat gcaggacagc
     3481 tttttcacaa tctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac
     3541 aaacggcaaa atatgaggaa ctatttttcc gtttttttct ctaatactca ggtcaggacg
     3601 ctgatttcac aacctctcga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
     3661 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttactact tgaaactaag
     3721 cagctttttc acaacctcta atacatggta ttccagaagt aacttcttcg aaaaataagc
     3781 cgaaatgtca caaaaatttg agaagcgatt ttcccaaatt ccttcttatt tcttgaagct
     3841 gaccacttct ggcacaacct cttaccagat tcgcactctt ttttctggcg ctagatacat
     3901 agcgtcttgt tccgtgatac caaatgtttc atagaattcg tcaaggtttt ttggttgcac
     3961 gtttgctcgg agtttagcag gggcatggac atcgatttgc agtaaaagtt gttggtattc
     4021 tttttttgct tttgtacgcc aaatcgttgc ccaattgaca aagaattcct cgatagaaag
     4081 atctgattcc gtttttgctg cttccaatgc acagcttaga ccgcctgcat cagcaatatt
     4141 ttccgaaacg gttaattttc cgtttacttt tccgtcagca aaaggcagac catcgaattc
     4201 ttcgatcatt gcttgtgctt tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt
     4261 gttcaagttg ccatattcgt caaataaagc gccgttatta tcaaatgcat gggaaatctc
     4321 atgagcgata acagctccga ttccaccata attagcagag cttgattgtt ctagactgta
     4381 aaatggtgct tgaaggatcg ctgccggaaa gacgatcaca ttccgaaatg gatgataata
     4441 agcattgacc gtgtcagcac tcatttccca ttctgaacga tccactggtt tgttccattt
     4501 gctgaaacga tcttttaatg tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc
     4561 accctgatct gcaggtactg ttttgaactg gtcgaataat gctgggatct tatcggggta
     4621 gcctacttgg atacctaagt tatttaattt tatgacggct ttttcttttg ttgcatcccc
     4681 aagccagtca ttttcttcta gacgtttttt ataaacggcg atcatttttt tcaccatttg
     4741 ttctacgtct tttttagcag cttcgccaaa atattttttt ccgtagtaat cgccaactac
     4801 ttgatcgaat tgtccagagg caaggtaata cgctgctttt ttctgaggca ttgcttcgtc
     4861 tgttccagac aatacgcgag aatagattcc gctgacttgg cggaattctt ctgatagata
     4921 accactaaga gaacgtactg ttttaatgat catccagctt ttgaataacg gaaaatgagc
     4981 ttctgtcagg atgtcattca aatgatcgaa gtacactgga tcagttacga tcactttatc
     5041 tggagttgtc ccaatcaaac ccgtgacaag ttttttcaag tccagtttat ctgtgtattt
     5101 aataaatgta tcaaaagatt gcggattata cattttgctg taatctgcgt tttcttctgc
     5161 actttttaca tgaggagcaa tcaatttatc aaattgcact gcttgttccg tgattgcttc
     5221 tgcctgactt ttttctttgc cggcaagctc taataactga acagccatat caaagaaaac
     5281 gcctattagt tgtgcaccgt tgggatggtc tgatgcataa tacgtcttat ctggaaggaa
     5341 taaagaaggc ggataagcaa agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac
     5401 atccagatta aatggcaatg gtaatgtatc caacagccat tctggaaatt gcttattcaa
     5461 ttcgtcgaaa gactgcaatg attcaacttt ttcgattata ggttgaagcg gttgagcatg
     5521 caactcattt cttcggtcat aatcgtttgc cagttgataa tatgcaagga aatgtttcat
     5581 tttttctgaa gacacttttt ctggatgggc aagcagatcg tctgtttctc ccatcaaaag
     5641 ttggtcgatc ccgtcaacta aatcttggaa accgcctgta gctggtttat ctgcagggat
     5701 ttttgctgtt ttttgccatt cttcattgac tgcttcatag aaatcttgtt tcaatcgttc
     5761 tttgttcatt catcgatcgc tccttttttg tttcgtgtgc ccattatctt acaaaatgaa
     5821 ggaattaaca aatagaacat aaaaaattga aaattcgaac aatcaaactg atttgttttt
     5881 ttcaatcctt cacttataat gaaaaaaagg aggcgatccc aactgaatca agtagtggat
     5941 aaacctttat atttcaaagt agataaaaaa attaaaaaat tagccagcac tcagcagctg
     6001 cagtcaagaa aaagtaaatt gcttttttta gctctggttt ttgaagatca aagttatgtg
     6061 attatcgatc aatcaggaca tccggtagaa tattctccag caaaatatac ttatcaagag
     6121 ggtctttctt gtaaaaaatg gaagctgatc aatgaagagc caatcgagtt atctagatgg
     6181 atcaacagaa aagaagatat tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc
     6241 gcaaattgct gggtgggatt gccagaagaa cgttttttga ggtataagaa atgggctacc
     6301 ccctcaggct atctttgtgg tacttacgcg gcagcagttc tccttgccta ttatcaagac
     6361 tatcgtaagg ggtggatgct tccaaatgag attcgcaaga aaaatacggc tgattcgttg
     6421 gttttgacga aggcattgcg tagccagatc caaccactag gacttcctac gattcctttt
     6481 caggttagta cagggatttc tagtttttta aagaagaatg gcaatcatga gcgtgcacga
     6541 gcaactcttc taggttcatg gcagcgagct acaaaacgta tacgtgaagg caaacctgtg
     6601 atgattggta ttttgaaagt acttggcagt acatatggca atcattgggt gacagcgtac
     6661 gcttattttg aaactgaaac aggagaacgt tattacaaag tacacgacaa ctggggcgat
     6721 tatcataaag tcattcctgc cagttggagt aacggaacag tttcacttcc ttaagaattt
     6781 gctgaaatta acagaaaatc ttggaaaaat atgttaaact atcgaagaat agagaagttt
     6841 tggggaggac tacatgagta ataagtatca ggatgacacg ttgaagattt ttagtttgaa
     6901 tggcaaccgt ccattagctg aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag
     6961 cgtcgtgaag caatttagtg atggcgagat ttccatcaat atcgaagaaa gtattcgagg
     7021 ggatcatgtg tatatcgttc aatcaacgaa tgcacctgtc aatgattact atatggaact
     7081 actgatcatg atcgatgcaa tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc
     7141 ttactatggg tatgctaggc aagaccgcac ggcgaaacct catgaaccga tcactgcgaa
     7201 attgatcgct aacttgatcg aagaagcagg ggcaactcga gtactgactt tagatctgca
     7261 cacggttcaa gtacaaggat tctttgatat tcctgtagat aatttattta cgatgccgtt
     7321 gtttgctcat tattaccgtc aattaggtct gacgggagat gacatcgtag ttgtttcgcc
     7381 aaaaaacagc ggtgttcagc gtgcacgtag tctttcagaa tatttgaaca gcacattagc
     7441 catcgtagat catgcagatg atgcttcaac tgagggtggt tatgtgatcg gtgatgttca
     7501 aggaaagaca tgtatcatgg tagatgatat cttgaacaca ggagcaacgc ttgcacgagc
     7561 agcaaatgta ttaaaagaaa acggcgcaaa agaagtatat gcctgtgcgt cacatggatt
     7621 attgtccgat catgcgaaaa gtattttaga caatgcacca atcaaagata tttgtatcac
     7681 tgattctgtt ttcacagaag aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc
     7741 agaattaatg ggtgaagcgg tcaaacgcat ccatgaaaat acaccgatga gtccgttatt
     7801 ccgtctggaa gaaaaagaat tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga
     7861 agtgagcagc ttcaaggagc tgcgacaaga cttttgtcac agctcctttt ttctatgccc
     7921 atgaaacgtc ctctagacaa aaatgagctc ttctttggtt tgttcgctat tttatgctag
     7981 aatgtgccta gcaataattt ggaagggatg aatgaagtga aaactattta tctggatcat
     8041 gcggcaacta cccccatgca tccacaagta attgaagcga tgacggcaat catgcaagac
     8101 acgtttggaa atccttcaag catacatgga tttggtcgcc acgcgcatga aaaattggaa
     8161 acagcaagac aaatcattgc agacagtctg caagcaaaac ctcacgagat catctttaac
     8221 agtgggggaa cagaagggga taatacagct atcttagaaa cagcgttctc gcgtcaaaaa
     8281 gaaggcagac atctaatcac tacagctatc gaacatcctg ctgttcttaa taccatgaaa
     8341 tatttagaga caaaggggtt tgacgttaca tatctgcctg tagatgaaaa agggaatctg
     8401 tcgatcgaag aggtcaaaaa agcgttacga gaagatacga tcttggtctc tgtgatgtac
     8461 gggaacaatg aaatcggaaa tttgatgcca atcaaagaaa tcggtgaact tttagcggat
     8521 catccagctt atttccatac agatgcggta caagcgtacg gaaaccaaac gatccatcca
     8581 gaagaattag gaatcgattt attaagtatc tctgctcata aaatcaatgg gccaaaaggc
     8641 gtcggtttct tatataaacg agacggagta aatatccctg cattgctttt aggcggggag
     8701 caggaagaaa agcggcgtgc aggtacagaa aatttagcag ggatctgggg tatggcaaaa
     8761 gcagttgaac tgctgtcacc tgaagaacgt gaagcacgta acaacaaata taaaggattc
     8821 cagcaattga ttcttgatac attagaagca aacgaaatcc cttatcaagt aaatggtgat
     8881 ttaacgaata aactccctca tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg
     8941 ctactgatga agcttgatct taatggggtc gctatttcta ctggttcagc ttgtacagca
     9001 ggaaatatcg agccttcaca cgtattagaa gcaatgtatg gagaagatac accgatacta
     9061 aaagaatcca tccgtatcag ctttggtttg ggaaataccg aagacgacat ccagtatttc
     9121 cttgatcaat tagttcgtgc tgtcaaaaaa taactagtga agtcattctt atatgaacag
     9181 atgtctgaga gaacgccttc attttttagg gaacttaact gaaaaaccta gtaaaaatca
     9241 ggataaactt ttataataaa gagaaactaa ttttatcgag gtgaaaatca tggcatttga
     9301 aacaacagca acggtactgg gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag
     9361 atatacattg cgcgataatg gatttacaga aacaaacgga ggcaattatc aattgatccg
     9421 accattagat gccacgccgc aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa
     9481 agatattcaa acattgaaaa tgtcgatcac tacagcaaat ggattgaaag cagtcaatat
     9541 tttcaaagat ccaaaacaga aaatgagcca agataaattt tattttctga tggatgggat
     9601 gattagccgt gggttattcg aaaaagtcga atgatctgtg gatataaaaa gacaactagt
     9661 tttgaaacgg cctattgtta gatgactaac tttgtaagga cgacgcaaat tagttgtctt
     9721 ttttattttt aaatttaaca catgt
//
LOCUS       DACCZY010000107         9520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCZY010000107 DACCZY010000000
VERSION     DACCZY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.110"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IVZ67_002267"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IVZ67_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8166306.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IVZ67_002268"
     CDS             1873..4539
                     /locus_tag="IVZ67_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8166307.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IVZ67_002269"
     CDS             4903..5655
                     /locus_tag="IVZ67_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8166308.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IVZ67_002270"
     CDS             complement(5677..6831)
                     /locus_tag="IVZ67_002270"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8166309.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IVZ67_002271"
     CDS             7016..9457
                     /locus_tag="IVZ67_002271"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8166310.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3077 a         1711 c         2033 g         2699 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg
//
LOCUS       DACCZY010000108         9476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCZY010000108 DACCZY010000000
VERSION     DACCZY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.112"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            31..1005
                     /locus_tag="IVZ67_002272"
     CDS             31..1005
                     /locus_tag="IVZ67_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8166311.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            987..1448
                     /locus_tag="IVZ67_002273"
     CDS             987..1448
                     /locus_tag="IVZ67_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8166312.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1498..1962
                     /locus_tag="IVZ67_002274"
     CDS             1498..1962
                     /locus_tag="IVZ67_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166313.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1976..2725
                     /locus_tag="IVZ67_002275"
     CDS             1976..2725
                     /locus_tag="IVZ67_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166314.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2725..3555
                     /locus_tag="IVZ67_002276"
     CDS             2725..3555
                     /locus_tag="IVZ67_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8166315.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3555..4202
                     /locus_tag="IVZ67_002277"
     CDS             3555..4202
                     /locus_tag="IVZ67_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8166316.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4300..5076)
                     /locus_tag="IVZ67_002278"
     CDS             complement(4300..5076)
                     /locus_tag="IVZ67_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8166317.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5220..6179
                     /locus_tag="IVZ67_002279"
     CDS             5220..6179
                     /locus_tag="IVZ67_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166318.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6296..6604
                     /locus_tag="IVZ67_002280"
     CDS             6296..6604
                     /locus_tag="IVZ67_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8166319.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6631..6930
                     /locus_tag="IVZ67_002281"
     CDS             6631..6930
                     /locus_tag="IVZ67_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8166320.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7104..7655
                     /locus_tag="IVZ67_002282"
     CDS             7104..7655
                     /locus_tag="IVZ67_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8166321.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7801..8094
                     /locus_tag="IVZ67_002283"
     CDS             7801..8094
                     /locus_tag="IVZ67_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166322.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8087..8383
                     /locus_tag="IVZ67_002284"
     CDS             8087..8383
                     /locus_tag="IVZ67_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166323.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8454..9458
                     /locus_tag="IVZ67_002285"
     CDS             8454..9458
                     /locus_tag="IVZ67_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8166324.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3345 a         1366 c         1780 g         2985 t
ORIGIN      
        1 ataatactta ttagagtgag ggataaagaa atgagacaac tacacaaaga acagattgaa
       61 acagcaatgg cagcgttaaa ggaaaagaca attgatcatg tatattttac agcatgtggg
      121 ggttcacaag ccgttttaat ggctggtcaa tatctatttg ataaagaatt aactgtacct
      181 tgtcatgttt atactgctaa cgaatttaac tatgctatac caaaaggatt atctgaaaat
      241 tctttagtta tcacttgttc ccattctggc acaacaccag aaactgtaga agcaacaaaa
      301 aaagcaagag aatttggagc aatgactatt tgtttatcta atgaagaagg atctcctcta
      361 tgggaagcag ctgaatatcc tgttcactat gattggggta aagatgttga tgctagtgat
      421 ttaaataaag gaattcttta tggtttatta tttaatattt tacttgtttt agatggctct
      481 aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt tattgctgaa
      541 aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg agataaaatt
      601 atttacacaa ttggtagcgg tatcaattac ggagaaattt actccacagc agcttgctgg
      661 ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt tttccatggt
      721 ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat tggagataca
      781 agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa attaatggtt
      841 ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga atatattgca
      901 gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt tgaaagaggc
      961 cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat aggaggttgc
     1021 aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc ctttgtgagg
     1081 gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt agtgctgttc
     1141 catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa ataattagtc
     1201 agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca cctaataatg
     1261 tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt aatttacctc
     1321 ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa atagatttta
     1381 aggaattact gaataactct caaaaaagta tggttcatat taattcgctt ttaaaagatg
     1441 tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg aaatagaatg
     1501 attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt ttcttggact
     1561 aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg agatccaatg
     1621 tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat tgttaaaatt
     1681 agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat tttaattaaa
     1741 ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga aattaatttt
     1801 ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt tttaactcct
     1861 gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt tgtgcaacaa
     1921 gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa aataaatgtt
     1981 attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact ccaggttaat
     2041 tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac ttgtgctagg
     2101 tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta tgggattcgt
     2161 ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg ccactgcttt
     2221 tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc cagtggctgt
     2281 agctggtgaa tttatgagta ttatcatgcg catgataatt gctcaatttg gacatgctgc
     2341 agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata tttattggtc
     2401 atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat attttggtgc
     2461 tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg ccttgactgt
     2521 ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa tgttgagtaa
     2581 aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag gtctttcact
     2641 aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag ttaaatatgg
     2701 aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg tttaatcaca
     2761 aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa tcatgtgttc gtggaattat
     2821 gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa taaagtttat
     2881 aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt ttttaactgt
     2941 acgcctcaaa tgacttcatt tattatggga ctttgtgctt ctatggaaga acaaaatgct
     3001 caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac cagtttaatg
     3061 ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat tattacattt
     3121 gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt agctgttggc
     3181 ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg atatgagtct
     3241 ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt aatgcaattt
     3301 gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt gccaattgtc
     3361 acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt acttgattcc
     3421 attattcctg gattattacc acttgtatgt actttgggaa tatataaatt gattaagaaa
     3481 aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt tttaagtata
     3541 ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt ttaattgata
     3601 gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat ttaagctatc
     3661 ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat gatatagaaa
     3721 agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa tattggaaat
     3781 cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag cttgaatggt
     3841 taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat actataaatg
     3901 aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc ggaagagatt
     3961 tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag ctcagaattc
     4021 cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct ggtaaaagag
     4081 caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta agtctagcag
     4141 atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa tttggaatat
     4201 aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat gcccaaatct
     4261 aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta ctttaggatc
     4321 tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa taacataact
     4381 aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat ttttatcttt
     4441 gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata agtcggaaag
     4501 ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt tgacatcata
     4561 gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa ataaagttgg
     4621 aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt cttcccacat
     4681 taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat tagctaaaaa
     4741 ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat gtcctcgggt
     4801 agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt ttttttgagt
     4861 tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgcta aaagtttaaa
     4921 agcttttcga atggtaatct tacttactcc ataaatttta cataattctt cttctgtagg
     4981 taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag aagcttctac
     5041 ttgtaaatat aatggtctat tatcttgaaa attcatagta cactccctaa aaagttatac
     5101 ttcattatat cacaatttat taaaacgtcg attgtaaaat taagctagac aactaaaaag
     5161 atcatttgtg ctacactcaa aatagttccg accaaagaat taataaggag tgagtacaaa
     5221 tgacttacac ccatcttaca acagacgaac tagtaatcat agagtcttat ttcaatatcg
     5281 agaaacctgt cgctcttgtc gcacaaagtt taaatcgctc tagacaaacc atttataatg
     5341 tttattcgtt tctcaaacaa ggaaaaaaag tgctagatta ttaccgtcaa tacaaacaaa
     5401 ataaatctcg ttgtggacga cgttcaaccg ttttatctga tgaacaatca acatatgttc
     5461 aagaaaaggt agctcaagga tggtcacccg atgtgattat cggccgtggc gagtttccaa
     5521 ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa aggccttttt gacacacagt
     5581 cgttacctat gaaggggaaa cgtaaaccga ataatcatca agaaaagcgt ggaaaacagg
     5641 cctttcgcag aacgattcat gaacggaaca gtctctatcc acacctgact aaagaatttg
     5701 gtcatctcga agtcgacacc attgtgggag ttaaacacaa gagcgctgtt attacattgg
     5761 ttgagtgttt atccaaggtt attattactt tgaaaccgac aggcagacga gccgaagata
     5821 tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa acacttgttt aaatccatga
     5881 catttgattg tggaaaagaa ttttccaact ggcgatctat aagtaactcg aacgatattg
     5941 ccatcttttt cgcagatcca ggaacacctt cgcaaagagg gctaaatgag cactcaaacg
     6001 gcttgcttag acgagatggc ttacctaaaa caatggactt tagggacacg gatgaaacct
     6061 ttattcaatc cgttgcctcg aaaagaaacc atattccaag aaaatcatta aattatcgaa
     6121 caccattgga agtatttttg agctgcatag ataaagacat attgtctagc ttaatttgac
     6181 aaatcagata gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc
     6241 ccaatcttct ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt
     6301 tcaaaataaa ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt
     6361 acaagcttta tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta
     6421 tcttcaaatc tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga
     6481 agaaccagaa gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc
     6541 tcaaaaaata tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata
     6601 ctgatatcta aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa
     6661 aagtgtgcac agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa
     6721 aaagaggaaa ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg
     6781 acacatcttt atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt
     6841 acagatatta ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt
     6901 tggagtcaga ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg
     6961 tttagaagat atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg
     7021 aaatattttc aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa
     7081 aaattagcat aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag
     7141 tttggcgaga tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag
     7201 gaaaagaagc ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac
     7261 catttacgaa tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta
     7321 ctggttttat tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc
     7381 ctcgtggaaa tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa
     7441 tcaacaccat tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac
     7501 aagcgaagac catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga
     7561 taaatgcaga aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat
     7621 tggcagatag tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat
     7681 aagtagtttt acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat
     7741 tatcaatgta cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag
     7801 atggagattg atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag
     7861 gaaatggaac tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag
     7921 atttcttttc caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg
     7981 atagaacagg aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca
     8041 agatggattc aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat
     8101 tgtattaggt ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc
     8161 gaatatagat aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc
     8221 tctaactaag aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg
     8281 atgtggaagg gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg
     8341 ttgtcatgct ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat
     8401 taattgactg ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa
     8461 aatggttgtt attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata
     8521 cgaggactaa agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc
     8581 aatcaactga acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa
     8641 gttcaatcat tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat
     8701 ctactcaact agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa
     8761 gctacaatcc tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg
     8821 atggaaattg gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg
     8881 gaactggtca tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat
     8941 atttagggca gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa
     9001 cgcaacaaaa tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac
     9061 agttaaaaca aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag
     9121 aagaatctac tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt
     9181 caagttggga agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt
     9241 ctcctgataa aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc
     9301 taatgcaaga tgaaaatgga agctactatt caattaatat tgtaacggat gataatggaa
     9361 atacaacaat ggctggtgga aaacaattcc gtgtttatac agatggaaga atttctcaac
     9421 gtattggaat gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatc
//
LOCUS       DACCZY010000109         9349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCZY010000109 DACCZY010000000
VERSION     DACCZY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.113"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(37..705)
                     /locus_tag="IVZ67_002286"
     CDS             complement(37..705)
                     /locus_tag="IVZ67_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8166325.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            839..1126
                     /locus_tag="IVZ67_002287"
     CDS             839..1126
                     /locus_tag="IVZ67_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166326.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1542..3080
                     /locus_tag="IVZ67_002288"
     CDS             1542..3080
                     /locus_tag="IVZ67_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8166327.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3077..4042
                     /locus_tag="IVZ67_002289"
     CDS             3077..4042
                     /locus_tag="IVZ67_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166328.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4029..5351
                     /locus_tag="IVZ67_002290"
     CDS             4029..5351
                     /locus_tag="IVZ67_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8166329.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5414..5518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5592..6335
                     /locus_tag="IVZ67_002291"
     CDS             5592..6335
                     /locus_tag="IVZ67_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166330.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6350..7342
                     /locus_tag="IVZ67_002292"
     CDS             6350..7342
                     /locus_tag="IVZ67_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8166331.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7354..8091
                     /locus_tag="IVZ67_002293"
     CDS             7354..8091
                     /locus_tag="IVZ67_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166332.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8081..8803
                     /locus_tag="IVZ67_002294"
     CDS             8081..8803
                     /locus_tag="IVZ67_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8166333.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8887..>9349)
                     /locus_tag="IVZ67_002295"
     CDS             complement(8887..>9349)
                     /locus_tag="IVZ67_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8166334.1"
                     /translation="AIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQ
                     AEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATN
                     GRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3064 a         1609 c         1974 g         2702 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      961 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
     1021 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1081 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1141 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
     1201 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
     1261 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
     1321 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
     1381 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
     1441 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
     1501 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
     1561 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
     1621 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
     1681 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
     1741 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
     1801 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
     1861 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
     1921 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
     1981 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
     2041 ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt
     2101 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     2161 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     2221 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     2281 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     2341 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     2401 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     2461 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     2521 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     2581 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     2641 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     2701 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     2761 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     2821 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     2881 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     2941 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     3001 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     3061 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     3121 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     3181 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     3241 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     3301 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     3361 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     3421 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     3481 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     3541 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     3601 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     3661 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     3721 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     3781 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     3841 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     3901 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     3961 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     4021 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     4081 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     4141 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     4201 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     4261 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     4321 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     4381 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     4441 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     4501 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     4561 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     4621 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     4681 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     4741 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     4801 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     4861 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     4921 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     4981 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     5041 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     5101 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     5161 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     5221 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     5281 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     5341 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     5401 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     5461 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     5521 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     5581 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     5641 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     5701 ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat
     5761 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     5821 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa
     5881 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     5941 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     6001 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     6061 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     6121 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     6181 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     6241 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     6301 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     6361 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     6421 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     6481 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     6541 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     6601 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     6661 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     6721 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     6781 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     6841 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     6901 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     6961 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     7021 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     7081 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     7141 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     7201 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     7261 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     7321 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     7381 gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc
     7441 tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     7501 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     7561 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     7621 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     7681 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     7741 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     7801 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     7861 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     7921 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     7981 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     8041 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     8101 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     8161 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     8221 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     8281 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     8341 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     8401 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     8461 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     8521 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     8581 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     8641 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga
     8701 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     8761 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     8821 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa
     8881 tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata
     8941 acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt
     9001 taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga
     9061 accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc
     9121 agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc
     9181 cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc
     9241 tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc
     9301 tgagtaaatc aggttaatat cagaaattga atttgattct gcaatggca
//
LOCUS       DACCZY010000110         9335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCZY010000110 DACCZY010000000
VERSION     DACCZY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.114"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            33..1292
                     /locus_tag="IVZ67_002296"
     CDS             33..1292
                     /locus_tag="IVZ67_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8166335.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1302..2174
                     /locus_tag="IVZ67_002297"
     CDS             1302..2174
                     /locus_tag="IVZ67_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8166336.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2186..3016
                     /locus_tag="IVZ67_002298"
     CDS             2186..3016
                     /locus_tag="IVZ67_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8166337.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            3056..5239
                     /locus_tag="IVZ67_002299"
     CDS             3056..5239
                     /locus_tag="IVZ67_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8166338.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            5300..6520
                     /locus_tag="IVZ67_002300"
     CDS             5300..6520
                     /locus_tag="IVZ67_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8166339.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            6517..7977
                     /gene="gtfA"
                     /locus_tag="IVZ67_002301"
     CDS             6517..7977
                     /gene="gtfA"
                     /locus_tag="IVZ67_002301"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8166340.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            8054..9160
                     /locus_tag="IVZ67_002302"
                     /pseudo
     CDS             8054..9160
                     /locus_tag="IVZ67_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         3146 a         1461 c         1834 g         2894 t
ORIGIN      
        1 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
       61 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
      121 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
      181 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
      241 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
      301 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
      361 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
      421 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
      481 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
      541 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
      601 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
      661 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
      721 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
      781 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
      841 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
      901 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
      961 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
     1021 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
     1081 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
     1141 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
     1201 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
     1261 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
     1321 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
     1381 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
     1441 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
     1501 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
     1561 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
     1621 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
     1681 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
     1741 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
     1801 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
     1861 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
     1921 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
     1981 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
     2041 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
     2101 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
     2161 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
     2221 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
     2281 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
     2341 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
     2401 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
     2461 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
     2521 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
     2581 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
     2641 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
     2701 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
     2761 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
     2821 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
     2881 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
     2941 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
     3001 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
     3061 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
     3121 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
     3181 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
     3241 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
     3301 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
     3361 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
     3421 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
     3481 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
     3541 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
     3601 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
     3661 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
     3721 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
     3781 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
     3841 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
     3901 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
     3961 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
     4021 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
     4081 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
     4141 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
     4201 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
     4261 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
     4321 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
     4381 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
     4441 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
     4501 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
     4561 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
     4621 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
     4681 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
     4741 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
     4801 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
     4861 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
     4921 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
     4981 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
     5041 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
     5101 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt
     5161 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
     5221 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
     5281 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
     5341 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
     5401 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
     5461 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
     5521 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
     5581 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
     5641 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
     5701 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
     5761 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
     5821 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
     5881 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
     5941 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
     6001 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
     6061 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
     6121 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
     6181 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
     6241 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
     6301 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
     6361 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
     6421 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
     6481 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
     6541 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
     6601 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
     6661 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
     6721 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
     6781 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
     6841 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
     6901 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
     6961 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
     7021 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
     7081 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
     7141 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
     7201 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
     7261 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
     7321 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
     7381 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
     7441 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
     7501 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
     7561 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
     7621 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
     7681 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
     7741 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
     7801 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
     7861 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
     7921 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
     7981 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
     8041 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
     8101 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
     8161 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
     8221 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
     8281 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
     8341 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
     8401 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
     8461 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
     8521 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
     8581 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
     8641 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
     8701 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
     8761 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
     8821 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
     8881 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
     8941 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
     9001 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
     9061 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
     9121 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
     9181 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
     9241 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
     9301 tgttttatgc aacactagtg atatttttta gaatt
//
LOCUS       DACCZY010000111         9112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCZY010000111 DACCZY010000000
VERSION     DACCZY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.115"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            387..1517
                     /locus_tag="IVZ67_002303"
     CDS             387..1517
                     /locus_tag="IVZ67_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8166341.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1609..2364
                     /locus_tag="IVZ67_002304"
     CDS             1609..2364
                     /locus_tag="IVZ67_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8166342.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2422..4461
                     /locus_tag="IVZ67_002305"
     CDS             2422..4461
                     /locus_tag="IVZ67_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8166343.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4570..5721)
                     /locus_tag="IVZ67_002306"
     CDS             complement(4570..5721)
                     /locus_tag="IVZ67_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8166344.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5875..6405)
                     /locus_tag="IVZ67_002307"
     CDS             complement(5875..6405)
                     /locus_tag="IVZ67_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166345.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6504..6854)
                     /locus_tag="IVZ67_002308"
     CDS             complement(6504..6854)
                     /locus_tag="IVZ67_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8166346.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7011..8540)
                     /gene="ahpF"
                     /locus_tag="IVZ67_002309"
     CDS             complement(7011..8540)
                     /gene="ahpF"
                     /locus_tag="IVZ67_002309"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8166347.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8550..9107)
                     /gene="ahpC"
                     /locus_tag="IVZ67_002310"
     CDS             complement(8550..9107)
                     /gene="ahpC"
                     /locus_tag="IVZ67_002310"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8166348.1"
                     /translation="MIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFV
                     CPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQI
                     SRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHP
                     GEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         3023 a         1724 c         1711 g         2654 t
ORIGIN      
        1 ctatttcatt tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa
       61 tagaatatag taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag
      121 aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata
      181 gctaagaatg ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag
      241 gtcagaattc ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta
      301 tttgatgaga caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc
      361 ggtgaaaaaa gatcgattgg tggattttgg gtccttattt gactttgtcc atgatcggtc
      421 ttttagaagt atacagtgcc agttcttatc gtttattgca agccgatgaa aatacaaaaa
      481 gtctattatt gcgtcaacta attttcatat ttttgagttg gagcgtgatc ttcttagctc
      541 gttcagtcaa actacactat ttacttcacc ctaaaatagc aggatacggt ttagccttat
      601 cgattttctt tttagtatta gtaagaatag ggatattcgg tgtcactgtc aacggcgcac
      661 aacgttggat ctctctgttt ggcattcaat tccagccttc tgaactggca aatctttttt
      721 tgatttttta tttaagctgg ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa
      781 aaccattcct gattacagta ggtataactt ttctgatttt atttcagcca aagattgctg
      841 gagcattgat gatcctttcg attgcgtggg tcatattttg ggcagcggcg gttccattta
      901 aaaaagggat ctatctaatc gttacttttt ctgcattgct gattggagca gcaggcgggg
      961 tattatattt aggaaataaa ggttggcttc cacagatgtt taatcatgct tatgaaagga
     1021 tcgcgacttt aagagattct tttatcgata gtcatggtgc gggctatcaa atgacccatt
     1081 cgttttatgc actatataat ggtggcatat ttgggcgggg attaggaaat agtatcacaa
     1141 aaaaagggta tcttccagag acagaaacag attttatttt ttcgattatt acagaagagc
     1201 ttgggctaat cggtgcattg tgtgtgcttt tcttattgtt ctcattatgc atgagaatct
     1261 tttgtttaag cagtcgctgt aaaaatcagc aagctggatt gtttctttta ggattcggaa
     1321 ctttgctatt cgttcagacg ataatgaacg tgggaagtat cgcggggctg atgccaatga
     1381 ctggtgtgcc attgccattt gttagctacg gaggaacaag ttatctgatc ctttctttag
     1441 gaatcggaat cacgttgaat atttcttcaa agatacaggc cgaagagttg gcgctctatc
     1501 gacctgaaaa acaataaaga gaaactgaga aactctcagt ttgaattatc ataaagtaca
     1561 atcttttatt tttcaagcga gaggttcttt tagaaagagg aattcgcaat gaaagtatct
     1621 caaaaaatca tattatctat tttgttactg cttacattag tagttggcta tttttcgatt
     1681 gagtttatcc atggattttc gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg
     1741 aaaagtatcc ctaccacact aaatgtggct ttgattggtt cagatgctcg ttcgaaagac
     1801 gaaaacggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
     1861 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
     1921 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
     1981 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
     2041 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
     2101 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
     2161 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
     2221 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
     2281 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
     2341 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     2401 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     2461 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     2521 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     2581 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     2641 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     2701 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     2761 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     2821 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     2881 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     2941 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     3001 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     3061 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     3121 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     3181 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     3241 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     3301 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     3361 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     3421 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     3481 acagcttttg acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc
     3541 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     3601 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     3661 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     3721 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     3781 gacagttctt ggggatctta tcaagcaacg cgtgtcagta gtgatgtatc acaagtagac
     3841 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     3901 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     3961 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     4021 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     4081 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     4141 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     4201 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     4261 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     4321 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     4381 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     4441 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     4501 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     4561 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     4621 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     4681 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     4741 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     4801 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     4861 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     4921 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     4981 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     5041 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     5101 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     5161 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     5221 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     5281 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     5341 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     5401 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     5461 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     5521 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     5581 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     5641 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     5701 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     5761 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     5821 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     5881 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     5941 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     6001 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     6061 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     6121 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     6181 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     6241 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     6301 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     6361 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     6421 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     6481 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     6541 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     6601 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     6661 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     6721 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     6781 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     6841 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     6901 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt
     6961 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
     7021 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
     7081 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
     7141 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttcc atctagccaa
     7201 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
     7261 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
     7321 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
     7381 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
     7441 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
     7501 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
     7561 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
     7621 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
     7681 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
     7741 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
     7801 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
     7861 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
     7921 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     7981 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     8041 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     8101 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     8161 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     8221 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     8281 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     8341 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     8401 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     8461 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     8521 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     8581 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     8641 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     8701 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     8761 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     8821 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     8881 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     8941 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     9001 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     9061 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga ca
//
LOCUS       DACCZY010000112         9027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCZY010000112 DACCZY010000000
VERSION     DACCZY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.116"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            62..1108
                     /locus_tag="IVZ67_002311"
     CDS             62..1108
                     /locus_tag="IVZ67_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166349.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1110..2369
                     /locus_tag="IVZ67_002312"
     CDS             1110..2369
                     /locus_tag="IVZ67_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8166350.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2375..2956
                     /locus_tag="IVZ67_002313"
     CDS             2375..2956
                     /locus_tag="IVZ67_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8166351.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3038..3913)
                     /locus_tag="IVZ67_002314"
     CDS             complement(3038..3913)
                     /locus_tag="IVZ67_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8166352.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4061..4393
                     /locus_tag="IVZ67_002315"
     CDS             4061..4393
                     /locus_tag="IVZ67_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8166353.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4505..5713
                     /locus_tag="IVZ67_002316"
     CDS             4505..5713
                     /locus_tag="IVZ67_002316"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8166354.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            5867..6322
                     /locus_tag="IVZ67_002317"
     CDS             5867..6322
                     /locus_tag="IVZ67_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166355.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            6684..6959
                     /locus_tag="IVZ67_002318"
     CDS             6684..6959
                     /locus_tag="IVZ67_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166356.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6972..>9027
                     /locus_tag="IVZ67_002319"
     CDS             6972..>9027
                     /locus_tag="IVZ67_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8166357.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKS"
BASE COUNT         3000 a         1506 c         1894 g         2627 t
ORIGIN      
        1 ttatcttttc tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg
       61 tatggaactt gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct
      121 agtcagtcag ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg
      181 ctattatgat ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga
      241 ctggtatatt tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga
      301 tttcgtcaaa cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg
      361 gatcatggat ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat
      421 gatccctgta tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca
      481 gctaccaaat cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca
      541 aacaatcagc gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg
      601 cggaatgatt tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa
      661 gcaattaacg actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt
      721 agaggcagaa aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga
      781 aaatggccat ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt
      841 gtggaatctc caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga
      901 cttgcttggc tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa
      961 agttcttttt ttggcagaaa aaagcaaaaa aatacttgtt ttagaaaaaa taggtataat
     1021 agacggatac caacatccaa acgaaaaaga attattgaga acaatgacgg tatttcaaca
     1081 aatcgtcagt gagataagag ggaattgaaa tgaatacaaa tagcgaaaaa atgttgcaag
     1141 ctttatcaga tgaagacttg tcacaagcac aaatcttttt agaaaaagca ttaaaagaag
     1201 acccagcaga tatattagct gaactaggcg aagaattgct ggctattggc tttttgatcg
     1261 aagcaaaaca gatttttgaa caactgatca accaataccc agaaaatgac ggactgaaca
     1321 tcccgttagc agaaattgca atcgaagata atgagatcga cttagctttt gaatacctgg
     1381 aaaaagtgga taagcaaagt gattactatc cgcaaagctt attggcgata gctgatttgt
     1441 accaagttat cggaatccct gaagtgagtg aagcaaaatt gaaagaagca gctgggttgc
     1501 ttcctgatga accattgatc cagtttgcgc tagctgaatt gtatttttca gttgatcgtt
     1561 tcaaagaggc agcagtggtt tatgaaacat tgttggcaag cgggataaat gaaatttcag
     1621 gaatctcgat gcaggaacgt ttaggacaat ccttaagtat gcaaggagaa ttcgaagcag
     1681 ctatcgtacc tttagaagaa gcactgaaag aagaaaggac agatgatcga ctgttccagc
     1741 tagcttttac gaatcttcaa ttgaaagaaa atcagcaggc aatcaactat ctggaagaac
     1801 ttcgagaagt taatccgcat tatcaatcgt tatatctata tctaggacaa gctcttcaag
     1861 aagaagaaat gattgaagaa gcacaaacag tgttagaaga aggaatcaaa gaaaatcctt
     1921 accaagtcga actttatcac ctggcctcgg aaaatgcttt ccgtctgcat gatagacagc
     1981 gagcagaaga acttttactg aaagctttag aagtaggaga caaacaagat gaaacattac
     2041 ttactttaag taatctttac ttggatgatg aaagatatga agatgtgata caagtgatca
     2101 atcagatgga agaaactgct aatccttatg cagaatggaa cctagcccat gcttataatg
     2161 aattagaaga ctttaccgtt gcagctgtac actatgaaca ggcttatcat gaactatcac
     2221 atgaaccaga tttcttgaaa gagtatgcct ttttcttaag agaagaaggg cagctgaaaa
     2281 gagcgcaaga attattgact tattatttaa cacttgaacc aggagatatg gaggcactgt
     2341 cattattaga tgacctaaca gagaggtgag aaaaatgcta attgatgtac gggaaaagaa
     2401 gaatttttta acgtggatgg tcaatcatgt atcttttagc cgaagagaag ttttttggat
     2461 cttgaattat cttgccaatc atgaagcgat tttaagcaat gtccactttg ttgaaggggc
     2521 gaattttgcg gatcgaggac tgcagatcag tgatctatct gttgaagggg aacccatgaa
     2581 attgtttttg cagggaaaag aatttacgga cacagaccag atttttcatg aaatccgttt
     2641 gaattggaaa aagcctttat atgtggaatg tatattcgaa aatgcttggc agacaagaga
     2701 atatctatct atcttagaag acaatccatt ttcttcttgg gattcttcag tcagtgaaga
     2761 agatactgaa ttaattgaac gatattttca agaaaaagaa gaagaggcaa aactaaaaat
     2821 gctttatgct caaatcgatc aagcactaga agatggagat agggatgctt ttttgaaatt
     2881 atctgatgaa gttaaccgaa agatcctttt gcagacacaa aagaaaagta accgggtaag
     2941 tgaacataac aattagttaa aaatagtata gtactaaaaa agacaaaggt cagctggatg
     3001 tggaatcttt cctcatagcc tatctttgtc ttttttctta tagttttttc agtaatcttg
     3061 tctgtggctt caaataagta aagccttgcc cttccacttc atgcacattg atgattgaaa
     3121 ggaaagcttt ttcgtcaatc tcgtggacga tcctcttagc ttcagttaat tcactagagc
     3181 taacgaccat atagatgatt ttttttgaat taccagagaa tgcgccttct ccagaaagga
     3241 aagtcacacc tctttgcaga ccggtcatca gtagtggtgc aatctcttcg cttttgttgg
     3301 agacgacaag tagacctttt gcagaatatc cgccatctaa gatcgaatca atcacacgac
     3361 tgaaaacata cgaaacaatc aacgtataca tcatacgttt caagtcgata taggttaatg
     3421 aagcaagtaa gacgatgata tcaaatgcca atagggaacg ccccatagtg atgccgtaat
     3481 tttgttctaa aatccgagcg ataatatcac tgccgcctgt tgttccaccc atgcggtaga
     3541 cgatcccgct tcctacacct gcaactaaac ccgcaagtag agaaacgatc agtagatcat
     3601 gctgtaaatt gatttctagt ggaacccttt gccagatcca taaaaagaat gacaaagaaa
     3661 gtgtacctag aacagtatag gccaaggaac gtttaccgag gatctttcct cctagtaaaa
     3721 tcaatgggat gttgattaat aaagtcgtat aagctggatc aataccaaat aaagctctta
     3781 agatcaaggt gatccctgag actccccctt ctgctaaatg attagcgatg ttcaagtaaa
     3841 caagaccaaa tgcataaata cttgttccta taatgataaa aagaacttct tttactgttt
     3901 ttcgtttgac catatgaatt ccagctttca attatgtatt ttcacagaat ttccatttag
     3961 aaattccttc ctaaatgtag catggaaatt cgattttctc aattgctctt atctaggatt
     4021 tgcgttaaag ttttggtaag atagttttgg agggaaaaag atggataaaa agcgttcttt
     4081 atacagtatg cagcaggaag tggatgaata tatccagcaa ttcaaggtgg ggtatttctc
     4141 tccattagct cagatggctc gattaactga agaagtaggt gaattggcaa gagaagtcaa
     4201 ccacacttat ggagaaaaaa gcaaaaaggc ttctgaacca gttaactcgg tagcagaaga
     4261 acttggtgat gttttattcg ttacgatgat catggctaat tcattgaata tcgacttaac
     4321 tgaagttttt gaaaaaaaca tggaaaaatt caatcaaaga gaccataatc gctttgagcg
     4381 taaggacgag taaacaagat gaagtggtcc tggccttgaa gactctgaaa cggacttgtc
     4441 aaacagtcac catgcaactg agtctttatc tcaggacaag aaacacgcag gaaaggttgg
     4501 aaaaatgaaa ttaaaacaat tacctctgga ataccaaaag gcaattccag ttctgaaaaa
     4561 attagagaat gcaggatatg aagcttattt tgtaggtgga agtgttcgtg atatcttgct
     4621 gaaccagcca atccatgatg tggatatcgc aacaagcgca tttccagaag aaatcaaaca
     4681 gctttttcca aaaacgatag atattgggat cgaacatgga actgtgttgg tattagaaaa
     4741 tgatgaacaa tatgagatca ccacatttcg gacggaatca acttatcagg actaccgtag
     4801 accagatcat gtcgaattcg ttcgttcgtt ggcagaagat ttgaaaagaa gagattttac
     4861 gatcaatgcg tttgctttga aagaagatgg agaaatcatc gatttgttcg atggtctgca
     4921 agatctagaa gaacagatct tgcgagctgt aggcaatcct catgagcggt tccatgaaga
     4981 tgccttacga atgatgcgcg ggttgcgttt tgtcagtcaa ctagggttca aattagagga
     5041 agaaaccttc tacgccatca aagaaaacca cgagctatta ggtaaaatat ctgtagaacg
     5101 tataaatgtg gaattcatca agatgctctt gggaaatgaa cgggccgcgg gaatccatgc
     5161 atttattgaa acagagtgct acatttgctg tcccggtttg aaagaagaag gcgaagcact
     5221 tttacgtttt gctgaattac catccgtccc attgaaagaa gagagccaag catgggcatt
     5281 attgatcagc caactggatc taaaagaaaa tgagattcgt tcctttttga aatcgtggaa
     5341 gtgttctaac caaatgatta aagacgttca agcacttgtc caaggactac gacaacgaga
     5401 aactggagct ttggaaccat tcgaattgta cttcttaggc aaaaaacatg cactgcaagt
     5461 agaagaattg atgatctatt ttgacgaaac acctcagatg gatgccgtag gagaatccta
     5521 tgatcgttta cctatcaagt cattaagcga attagcagta gatggtaaaa tcttgctaga
     5581 agaaactgga aaaaaacctg gtaagtgggt tagtgaagct ttgcaattag ctgaaaaagc
     5641 cgtcgtagaa caaagaataa aaaatgataa aaaacaggtt ttggattttt tatcgaaaga
     5701 aaaattgatt taaaaaacac tagactgtat ctgctcagct ttgttggcta cttaaagtgg
     5761 gcagatacgt cattagaaga aaaaacataa gatgtgacca cttacataaa tttgtgctaa
     5821 actgaggtag tacaagaata aattacatca taaaggagtc tttttaatga gtaaagaaat
     5881 gacagcgcta aaattttatt ttagaaatgg agaaacctgg acgatcaacc gccgtcatat
     5941 cggcgatctt tggatcaaac aaatcacaac aagttttgga cgaatcaatg gcagtgaatt
     6001 tgttgaaatc catccgtgtg ctggatttaa aatcgaaatc ttccatgaag gggacgcagt
     6061 tgcgacacat gatatcaacc taggcggact ggaaatgggc atgtttaatc gcgcattgaa
     6121 atacgaagat atcgagcgaa tggaaatcct ttaccgaaat ggcacacctg atcttgtgta
     6181 cttcccatat ctagataaag gaacagaagg attagacaat caatatcaat caacaaaaat
     6241 cagcgaaaaa acaggtaact tgtatatcgt gatcaatcca gatcaacgtg tagaagatgt
     6301 atacggggaa tttttcgaat aaatcgattt ttacgcaaaa atgaggctgt gaaagagatg
     6361 tgtcttatta ctactgatag gctgtctctt ccacaccgtt tattcagaat aagggactgt
     6421 gacaagattt ttgtcgcagc cccatttttt tgttgtgata aaagaacaag aaaggcagga
     6481 atgggaaagt aaacatcctt ttgtcagaaa cttttatctt tcttaagttt tacttgatat
     6541 ctgatactat ttggaataga atgaggcgtt gaaaaattta tggaaatttc taactagcaa
     6601 taaacgatga aacaatgaaa tatttttcat ccaaaaagaa ctttgctata atgatggaag
     6661 tttgtgtata ggagatctgg aaaatgagag agagttttga acaacaaaaa aaactgttgc
     6721 atgatcgcta tggcgccctt tcgatggatg accgccgtca aatactttgt aagttgcgta
     6781 aaagaaatat attgatgtat cgtcagttgg aacgtctgaa acatgatctg cttcgtttgg
     6841 agtcaaaacg tgtacagtgt gaattagagg ggaaccaaac acaggttgaa gtagtcgaaa
     6901 ccaaaatttt aaagaaaaaa gaacaatttt taaaaatgct tacacaaaat aaaaaataaa
     6961 ggggatggac gatggaattt gctgagttag ttattgtatt tgcagtaacg atcaccttat
     7021 cgaacatagc tagccgcatt ttgccaatga ttccagctcc actgatccaa attttccttg
     7081 gagtggtatt aggtctgaca gagtggggac aatcaatcga ttttgaacct gaattgttct
     7141 tagttatgat tattgcacca ttgttattta gagaaggaga aaaagcagat atttcttcga
     7201 ttctcaaaaa tttcgggaca attttgttct tggcatttgg aggagtcatt ttgacccttg
     7261 tcggggtagg tgcgacgtta tcattcctgt tgccaagtgt cccacttgct gcctgctttg
     7321 cttttggagc agcgttaggt ccgacagatg cggtagcagt cagttctctt tctggacgag
     7381 tgaatatccc taaaaaggcg atgcatattt tggaaggaga aggtcttttg aatgatgcat
     7441 ctggtgttac tgccttccag tttgctcttg gtgcgctgat caccggatct ttttcagcag
     7501 taaacgcggg gatgagtcta gtagtttcta gtattggggg agctttgatc ggctttttgc
     7561 ttgtctggtt caaacaaaaa ataattcatt tgatcgaaaa agcatcagca caagatgtca
     7621 cagggtatct gctgatcgaa cttttgcttc cttttctagc ctatgtattg gcagaggcat
     7681 ttgatgtatc tggaatcatt gctgcagtgg cagcaggtat tctccaagct tctggtttta
     7741 ggaaaatctc tgtgtttgat gcagaattgt cgagcttgtc ccacagtaca tggacaacta
     7801 ttgcttttac actgaatgca ttagtattta tatttttagg aatcgaattg acgcaagtgt
     7861 tctcgccagt atggggagac ggtttatatc caaacggtct tttattagcg attatcgtat
     7921 taatctctgt catgcttttt gtgatcagat tcatttctat cagtcttttt tatgtattca
     7981 aagatggcag taaaaagttc aaaaaacagt tgaacgagat cttgatctta acatttggcg
     8041 gtgtaaaggg aacagtcagt ttggctacga tttttatttt gccattttca atcaataata
     8101 tgatgtttta ccagcggtct ttgcttttgt ttctgacggc gggtgtcatc ttagtgactt
     8161 tggtcatagg gatcattgtc ttgcctatgc tgacggaaac agaggaagcg aaaagcacgg
     8221 atttgaatgc tttgatgatt ttagaagagg tcgttgaagt tttgcgaaaa gaaataaagg
     8281 aaattgatcg taatacaaaa gaatttttag ccactgaagc agtgatagaa aattatcaag
     8341 aacggatacg tgatctgtac ttggaagatt taaccgatga tgaaaagcaa gaagtacaag
     8401 aaatacaagc actgattttg tcgatcgaac gggatggatt ggatgaaagt tatcgttcgg
     8461 gcaagctttc ttctaatggt tatcgttttt actcccgttt tctttctcga ttcgaacatt
     8521 ctatcacaag ccagattttg tcttttatcg gcttttggtt cattgttgtc cgtaggttaa
     8581 tgcgaatcgt tctccatccg aaaatgtttt ggcaaagaag atatgctgat cgtcagacat
     8641 ttattagtga tgaagatatc caagaaatca gagagacata tcaaaaaaat actcagttga
     8701 ttattgaaag tttagataac ttgactgata tttatgatga aacgatggtt caatttttta
     8761 tccagcaacg caaacttgaa ggaataaaaa tgaactcggg gaatttgatt tcttcgtgga
     8821 tgatccagca agatagtctg tttacgaaaa aaatgctaag aggttattat cttgaaagaa
     8881 aaatcattga tgaatacgaa gttgctgaaa aaatcacgac gttttctgcg aacagctatc
     8941 gcaggaatat caatttgtta gaatcttata cgatgaacaa accatctgat aacttttctt
     9001 tccgatttgc gtttcgagca aagtcaa
//
LOCUS       DACCZY010000113         8961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCZY010000113 DACCZY010000000
VERSION     DACCZY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.117"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            278..787
                     /locus_tag="IVZ67_002320"
     CDS             278..787
                     /locus_tag="IVZ67_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8166358.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVZ67_002321"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVZ67_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8166359.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVZ67_002322"
     CDS             2322..3302
                     /locus_tag="IVZ67_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166360.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IVZ67_002323"
     CDS             3424..4842
                     /locus_tag="IVZ67_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8166361.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IVZ67_002324"
     CDS             4913..6382
                     /locus_tag="IVZ67_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8166362.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IVZ67_002325"
     CDS             6392..6859
                     /locus_tag="IVZ67_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8166363.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IVZ67_002326"
     CDS             6895..7767
                     /locus_tag="IVZ67_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8166364.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8961
                     /locus_tag="IVZ67_002327"
     CDS             7848..>8961
                     /locus_tag="IVZ67_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8166365.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQ"
BASE COUNT         3087 a         1332 c         1799 g         2743 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa t
//
LOCUS       DACCZY010000114         8886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCZY010000114 DACCZY010000000
VERSION     DACCZY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.118"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            63..1277
                     /locus_tag="IVZ67_002328"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IVZ67_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IVZ67_002329"
     CDS             complement(1456..1791)
                     /locus_tag="IVZ67_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8166366.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IVZ67_002330"
     CDS             complement(1775..2047)
                     /locus_tag="IVZ67_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8166367.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IVZ67_002331"
     CDS             complement(2498..3301)
                     /locus_tag="IVZ67_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8166368.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IVZ67_002332"
     CDS             complement(3279..4403)
                     /locus_tag="IVZ67_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166369.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IVZ67_002333"
     CDS             complement(4419..5234)
                     /locus_tag="IVZ67_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8166370.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IVZ67_002334"
     CDS             complement(5224..6105)
                     /locus_tag="IVZ67_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8166371.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IVZ67_002335"
     CDS             complement(6181..7470)
                     /locus_tag="IVZ67_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8166372.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IVZ67_002336"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IVZ67_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2755 a         1892 c         1416 g         2823 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca gccagactgt agacaaaagc tgttccaaac
     8881 tcgtaa
//
LOCUS       DACCZY010000115         8833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCZY010000115 DACCZY010000000
VERSION     DACCZY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.119"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            304..1629
                     /locus_tag="IVZ67_002337"
     CDS             304..1629
                     /locus_tag="IVZ67_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8166373.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1622..1972
                     /locus_tag="IVZ67_002338"
     CDS             1622..1972
                     /locus_tag="IVZ67_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166374.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1954..2139
                     /locus_tag="IVZ67_002339"
     CDS             1954..2139
                     /locus_tag="IVZ67_002339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166375.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(3043..3318)
                     /locus_tag="IVZ67_002340"
     CDS             complement(3043..3318)
                     /locus_tag="IVZ67_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8166376.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3290..4243)
                     /locus_tag="IVZ67_002341"
     CDS             complement(3290..4243)
                     /locus_tag="IVZ67_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8166377.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4855..6348
                     /locus_tag="IVZ67_002342"
     CDS             4855..6348
                     /locus_tag="IVZ67_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8166378.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6483..6779
                     /locus_tag="IVZ67_002343"
     CDS             6483..6779
                     /locus_tag="IVZ67_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8166379.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6880..7731
                     /locus_tag="IVZ67_002344"
     CDS             6880..7731
                     /locus_tag="IVZ67_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166380.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7752..7964
                     /locus_tag="IVZ67_002345"
     CDS             7752..7964
                     /locus_tag="IVZ67_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166381.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            8397..>8833
                     /locus_tag="IVZ67_002346"
     CDS             8397..>8833
                     /locus_tag="IVZ67_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8166382.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT         3067 a         1368 c         1664 g         2734 t
ORIGIN      
        1 aaactattaa gaaactgaat actcctattt tttgttttta aggaaagttt tttctttttt
       61 gaatttctta tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc
      121 aaattttcac aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt
      181 tacaaatcaa cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta
      241 atcgattctt ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta
      301 tttatgatgg attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc
      361 ttttacgcaa gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc
      421 gtcatgagtc gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca
      481 aagaagaagt acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct
      541 aaaacgttac atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta
      601 aacaatattt tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa
      661 tcgatcttaa aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa
      721 cggagaaaga agctggctca aatgatccag gagaatatta aagaagagct aggattgctt
      781 gctacagtag gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca
      841 aaacataatg atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg
      901 aatattcctg aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac
      961 caaattggta tcatgagtat taaagactta gctaattgga atccatacac tattaagagc
     1021 catttaggtg tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat
     1081 atagcgatcc caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca
     1141 cgagattata ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta
     1201 gctattcgta tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg
     1261 tcgatcttag aaacgagacc aggtttttca caccagatga agatccctat aacagataat
     1321 acaaaggaat tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa
     1381 gtaaggcacg ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat
     1441 ttatttagtg atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag
     1501 attcggcaaa aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct
     1561 cgtagtatta ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa
     1621 aatgattgat tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca
     1681 tactgaaaaa attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca
     1741 aatgacgact gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt
     1801 atgtattcaa aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa
     1861 gatcttaggt agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga
     1921 aatacgtcat atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc
     1981 cagcaatatg agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat
     2041 ggccagcgtt tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca
     2101 gcaggatacc ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc
     2161 ttttatgttt tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata
     2221 taaaaaaact agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac
     2281 taaaatagca ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac
     2341 cagcttattc agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg
     2401 tactacgaat aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat
     2461 tactaaacat tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat
     2521 cagctttatg atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat
     2581 taattctcct ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc
     2641 ttcatttgat tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt
     2701 attataacat aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc
     2761 acaaacgatt tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta
     2821 tatgtttgtt aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact
     2881 atattaaata tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata
     2941 cccccaaaca atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag
     3001 tatactaaat atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct
     3061 attatatata atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt
     3121 gactatttca tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac
     3181 gggaggatct acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc
     3241 gacattaata tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt
     3301 cgttggtaat aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca
     3361 ttgagtcagc atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt
     3421 attcaattct ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat
     3481 ctacataatc atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt
     3541 taacaatgct tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg
     3601 cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat
     3661 gagactcttc aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc
     3721 cttccattgc gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga
     3781 atgcacgaac cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt
     3841 ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt
     3901 tttcggacaa gtattcatta agttcttcag atccaggaga gccagccata atatataaat
     3961 gtggacttac ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg
     4021 atcgatcaaa gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt
     4081 ggtcataatc aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata
     4141 aatcattgtc tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc
     4201 ctagtttatc tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc
     4261 tatactaaat atatttagta tacttcatat acttaatata ttcgatatat ctaatatact
     4321 ccaaattgat aagaattcag actttataag gaatatagtg gatatactaa atatatttgc
     4381 tatattcagt atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga
     4441 caatttagag tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt
     4501 acaaagtagc aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg
     4561 gaaataattg attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc
     4621 acgctcgtct caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat
     4681 accttttccg attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt
     4741 tatttagtgt acttaaatta aatattctta aacactgaag agagaacaca tactatggct
     4801 gagattaaag ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg
     4861 agcgaaattt attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc
     4921 accatgaaac ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt
     4981 agcaagcaat taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta
     5041 gagaacgaaa agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg
     5101 gatgattatc gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca
     5161 tttgtagttg atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta
     5221 gatcaagtgg gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat
     5281 tttgtactaa agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta
     5341 gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat
     5401 ctaggatgta accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa
     5461 gaaatgaact atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca
     5521 gatcaaaaaa caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa
     5581 attcgtcaag tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc
     5641 ggagaaggac gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct
     5701 tcaggttatg cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag
     5761 gaaccgctat ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat
     5821 caagcagcaa gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta
     5881 aaaaataatg atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat
     5941 caaagaaaat atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa
     6001 aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta
     6061 acgatgccca agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc
     6121 ttctacaagg taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg
     6181 ttcgcgaggg ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca
     6241 gaggtatttg gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc
     6301 ttgaaccctc ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt
     6361 gcaaattgcc ttatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag
     6421 ctacgtgtag gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt
     6481 gtatgtcgtt aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt
     6541 tgggattatc tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt
     6601 cttgggttac cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt
     6661 ccttagcttc tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg
     6721 agtatattga gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt
     6781 agtttttatt tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa
     6841 ggaaaacgtt aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt
     6901 gaacacacct ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa
     6961 ttatggccat taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt
     7021 tactcgattg gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca
     7081 gcatcagaca tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt
     7141 actcaattag tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc
     7201 ctattcgatt taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa
     7261 tctggaagaa caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat
     7321 ggacacatgt atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg
     7381 aaacttatta aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt
     7441 ggttatgagt acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt
     7501 cacacgagta tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag
     7561 gaaatgaaag caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc
     7621 ctacgatatg gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca
     7681 tacatttttt ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga
     7741 atcgtttgaa aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa
     7801 ccttaaagaa ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta
     7861 aaacgggtac gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg
     7921 attttttgtt gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca
     7981 attgattgat gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc
     8041 tttatttgta aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag
     8101 gctgtgccta ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata
     8161 gtgaaaggat agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc
     8221 cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg
     8281 atgagaaaat cgtaagagaa attgatcgaa gatgtgacga acggttctgt tgcaaagttt
     8341 taaatctact atcaaataag gtagaataat agaaaaagat agcaggagga atgacgatga
     8401 atccttttaa aggaaagcaa tttcagcagg atgtgattat tgtagccgtg ggctactatc
     8461 ttcgttataa ccttagctat cgtgaagttc aagaaatctt atatgatcgt ggcattaacg
     8521 tttctcatac gacgatttat cgttgggtgc aagaatatgg caaactactc tatcaaattt
     8581 ggaaaaagaa aaataaaaaa tccttttatt catggaaaat ggatgaaacg tacatcaaaa
     8641 ttaaaggaaa atggcattat ttgtatcgag ccatcgatgc agatggttta accttggata
     8701 tttggttacg taaaaaacgg gacacacaag cagcctatgc ttttcttaag cggttagtga
     8761 agcagtttga tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct
     8821 ttaagaaact aaa
//
LOCUS       DACCZY010000116         8796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCZY010000116 DACCZY010000000
VERSION     DACCZY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.120"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            8..307
                     /locus_tag="IVZ67_002347"
     CDS             8..307
                     /locus_tag="IVZ67_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166383.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(404..838)
                     /locus_tag="IVZ67_002348"
     CDS             complement(404..838)
                     /locus_tag="IVZ67_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8166384.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1070..1780)
                     /locus_tag="IVZ67_002349"
     CDS             complement(1070..1780)
                     /locus_tag="IVZ67_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8166385.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1770..2630)
                     /locus_tag="IVZ67_002350"
     CDS             complement(1770..2630)
                     /locus_tag="IVZ67_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8166386.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2635..3273)
                     /locus_tag="IVZ67_002351"
     CDS             complement(2635..3273)
                     /locus_tag="IVZ67_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8166387.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3288..3587)
                     /locus_tag="IVZ67_002352"
     CDS             complement(3288..3587)
                     /locus_tag="IVZ67_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166388.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3630..5114)
                     /locus_tag="IVZ67_002353"
     CDS             complement(3630..5114)
                     /locus_tag="IVZ67_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8166389.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5134..5595)
                     /locus_tag="IVZ67_002354"
     CDS             complement(5134..5595)
                     /locus_tag="IVZ67_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8166390.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="IVZ67_002355"
     CDS             complement(5613..6680)
                     /gene="ulaG"
                     /locus_tag="IVZ67_002355"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8166391.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6898..7668
                     /locus_tag="IVZ67_002356"
     CDS             6898..7668
                     /locus_tag="IVZ67_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8166392.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7941..8768)
                     /locus_tag="IVZ67_002357"
     CDS             complement(7941..8768)
                     /locus_tag="IVZ67_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166393.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2663 a         1853 c         1383 g         2897 t
ORIGIN      
        1 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
       61 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
      121 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
      181 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
      241 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
      301 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
      361 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
      421 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
      481 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
      541 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
      601 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
      661 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
      721 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
      781 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
      841 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
      901 atcacatata actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta
      961 gaatgcatac tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     1021 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     1081 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     1141 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     1201 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     1261 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     1321 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     1381 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     1441 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     1501 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     1561 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     1621 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     1681 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     1741 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     1801 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     1861 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     1921 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     1981 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     2041 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     2101 tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     2161 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     2221 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     2281 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     2341 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     2401 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     2461 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     2521 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     2581 catgaaatat ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc
     2641 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     2701 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     2761 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     2821 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     2881 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     2941 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     3001 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     3061 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     3121 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     3181 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     3241 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     3301 tggtaccact ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg
     3361 ataccgataa ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact
     3421 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     3481 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     3541 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     3601 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     3661 tagtcttcta caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata
     3721 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     3781 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac
     3841 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     3901 gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca
     3961 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     4021 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     4081 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     4141 atcgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     4201 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     4261 aaactctgcc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     4321 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     4381 atcgacaaga atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta
     4441 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     4501 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     4561 actgttaagt tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat
     4621 gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     4681 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     4741 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     4801 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     4861 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta
     4921 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     4981 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     5041 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     5101 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     5161 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     5221 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa
     5281 aaacaaacta gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt
     5341 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     5401 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     5461 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     5521 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     5581 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     5641 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     5701 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     5761 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta
     5821 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     5881 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     5941 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     6001 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     6061 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     6121 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     6181 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     6241 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     6301 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     6361 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     6421 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     6481 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     6541 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     6601 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     6661 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     6721 aatattaatg tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat
     6781 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     6841 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     6901 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     6961 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     7021 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     7081 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     7141 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     7201 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     7261 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     7321 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     7381 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     7441 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     7501 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     7561 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     7621 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     7681 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     7741 cgctacgctc aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata
     7801 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     7861 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     7921 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     7981 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     8041 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     8101 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     8161 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     8221 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     8281 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
     8341 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
     8401 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
     8461 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
     8521 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
     8581 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
     8641 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
     8701 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
     8761 ttaatcaaaa caatttcctt ccctttaaaa aattat
//
LOCUS       DACCZY010000117         8728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCZY010000117 DACCZY010000000
VERSION     DACCZY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.121"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(687..980)
                     /locus_tag="IVZ67_002358"
     CDS             complement(687..980)
                     /locus_tag="IVZ67_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8166394.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1383..2561)
                     /locus_tag="IVZ67_002359"
     CDS             complement(1383..2561)
                     /locus_tag="IVZ67_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166395.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2665..2979)
                     /locus_tag="IVZ67_002360"
     CDS             complement(2665..2979)
                     /locus_tag="IVZ67_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166396.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3086..3301
                     /locus_tag="IVZ67_002361"
     CDS             3086..3301
                     /locus_tag="IVZ67_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166397.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3601..3843)
                     /locus_tag="IVZ67_002362"
     CDS             complement(3601..3843)
                     /locus_tag="IVZ67_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166398.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3830..4258)
                     /locus_tag="IVZ67_002363"
     CDS             complement(3830..4258)
                     /locus_tag="IVZ67_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8166399.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            complement(4410..5957)
                     /locus_tag="IVZ67_002364"
     CDS             complement(4410..5957)
                     /locus_tag="IVZ67_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8166400.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(6059..6412)
                     /gene="tnpB"
                     /locus_tag="IVZ67_002365"
     CDS             complement(6059..6412)
                     /gene="tnpB"
                     /locus_tag="IVZ67_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8166401.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(6402..6596)
                     /locus_tag="IVZ67_002366"
     CDS             complement(6402..6596)
                     /locus_tag="IVZ67_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166402.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(6822..8024)
                     /locus_tag="IVZ67_002367"
     CDS             complement(6822..8024)
                     /locus_tag="IVZ67_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166403.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(8027..8221)
                     /locus_tag="IVZ67_002368"
     CDS             complement(8027..8221)
                     /locus_tag="IVZ67_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8166404.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(8268..>8728)
                     /locus_tag="IVZ67_002369"
     CDS             complement(8268..>8728)
                     /locus_tag="IVZ67_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8166405.1"
                     /translation="KTKKANLKYLATWEKQQRGAIHYHIILFDFPFISFNRLTTIWGQ
                     GFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNLKLPTVTKKMVKKPY
                     DSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVHYTKIKK"
BASE COUNT         2599 a         1702 c         1417 g         3010 t
ORIGIN      
        1 taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt
       61 gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat
      121 ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg
      181 actgcagtgt tgtgtccctc atcacgaata aacggtgttc aattagctga tcttgtattc
      241 acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg
      301 acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga
      361 aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg
      421 gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt
      481 ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct
      541 cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga
      601 tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt
      661 ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga
      721 ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa
      781 tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact
      841 ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt
      901 ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc
      961 tccgtataag tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt
     1021 tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc
     1081 tctttttttt actactactt ataggtagat ttattctaat ccattattct cccctaccaa
     1141 taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt
     1201 gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac
     1261 ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg actacatatt
     1321 gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt
     1381 ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag
     1441 tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc
     1501 cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat
     1561 ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga
     1621 cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt ttcttctata
     1681 ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg tagcttttcc
     1741 ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt agtttcaatt
     1801 cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat ttcatttctt
     1861 ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca gccagtgtaa
     1921 aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc ataattcaaa
     1981 aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat ggcttctttt
     2041 aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg attttcactt
     2101 atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat aagtgattgt
     2161 aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt taaggtaaaa
     2221 agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc aatccatcga
     2281 acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg ttcatttaac
     2341 gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt gcttgcaaca
     2401 atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg cgcctcacgt
     2461 tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt gatcctacca
     2521 gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt cctcctattt
     2581 tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc atcattgtac
     2641 cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt tttctcgata
     2701 gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat agtgtcctcg
     2761 aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca gtgtactatc
     2821 acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta aatgactgac
     2881 tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat agaactctat
     2941 ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct ctcaaaatca
     3001 aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact ccatcaaaac
     3061 ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa aaataattaa
     3121 agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa tcaacttgag
     3181 tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc ttgccgatgc
     3241 tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa tatggaaata
     3301 gtagtttttc tgatctatca gctgttttgc caaagcacat aggaatctca cctcccccca
     3361 gtaatggcgg gccgatttgg ttacggaagt atcattatcg tagtcgcgat tctcatatta
     3421 aaatttgtcg cttaaaagct aaccttgtca tggcgtaact agagaatcaa aatctacatg
     3481 tactgataga tttgctattc atttgtcaaa gagcgctagg ctattttcag cctatggaaa
     3541 tcctttattt tgacccgata aaaaacttcc atagaatgaa aaggaaaggg aaaaaatact
     3601 ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat tttgtttcca gttgtctacg
     3661 catatattca tctacataca taaccattcc tttttcttct tcataaactg gtttcatggc
     3721 taaccgtgaa atataatttt gatattttga cagaacgatg gacatggcaa ttgaatcacc
     3781 agaagctgcg gctttgattg tcgttagtgg tatgaatggt atatatcgtt tattttgctt
     3841 catatttgac acccaaatac tttcctagct cttccaaagc catttgtcgt tggtaccaaa
     3901 cactgccgag acttttccca ataatatcgc caatttcacg atcattaaga ccgatgaaga
     3961 aggacataaa aatgatttct tgtctttgac taggtaatgc tgcaatcgca tttgccaact
     4021 cctcattgga aataaagatt ttcataccaa ggacaataaa agtaccacta ctattcaaat
     4081 ttggttccaa ataatagaac tctgtaccta accctttttg ttccaatatt tccattgata
     4141 tttgccgttg gcgaaatcta gcgtacttct tttgaatatt taacgcctca ttgcagacta
     4201 ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag tcgcccataa
     4261 tattctcccc ctcaaaaaat tcattcaact cgttttactt ctatcccttt ccattattac
     4321 tacaatctag gaggtaagcg ccccatagaa cagtacctgt acgtcaaata acccgaccta
     4381 tagaatagaa tcgggaatgt ttggcgtttt tagttacgag aaaaatgttg ttgtacatcc
     4441 ttgttccaag gcaacagttg atctaaagtt tctgctgaaa gtacgggaag atttggtatc
     4501 ttatcaaata gaaactctag atactttaca ggattcaatc cgttaagttc tgctgtttta
     4561 tatacactca gaatgattcc tgaagttcgt gctccttcaa gacttgtgga atgcaaccaa
     4621 tttttccgtc ctatcaccag ctctttgata tttcgctccg cacgattatt tgtgagttct
     4681 aaacgaccat cttcaaaaat aggttcaaaa ccacttcgta gtttacacgc atattgaacg
     4741 gctgcatcca acttggattt tgcaacagga tctatcgttg ccatccactc ataaaaagca
     4801 ataaataatg gacgcagttc ttgttgccga cagcttaatc gcttctcagg agaaaagtct
     4861 ttccatttct tttctaggac aaaccattta tccaattgtt tgactgcttg agctgcggga
     4921 atatccgtgt ttttctttcc ttttggaatt gcttcgaaaa atttccgccg aacatgcgcg
     4981 aaacaataga caagtcgtac actttccacg gcattatacc cgctatagcc gtcacagtgt
     5041 aaataccctg taaactcctt gagaaaagtt ttcggaacat ctccagcacg actttcgtcg
     5101 tggtgataat aaacgatggg atgctgactg tcttttcctg tacaaaactg ccagtaatag
     5161 gtatctgttt tggcactttc aatcacacga taacttgttt catccccgtg tagaatctct
     5221 tcttgattaa gatagactct taatcgttct cctaaagggt ctaacgcgtt ttgcactgct
     5281 ttaatatgcc aattggtgat attatctctc gaaatatcca acccatgtgt ttgtttccaa
     5341 tagttctctt gccgataggc aggaactttc tgaccaaatt tcatccggat cgtttccgcc
     5401 actaaactat tggaacctaa gctattagaa atcaaaggtt taggaacgat cgctttttta
     5461 aaagaagaaa gtccttcttt ttcgcagctt ttacaacaat aggtatgttg ataatgggtg
     5521 agacaatcca acatagcctg atggaaacga atttcttctc gtacttttgt ccgtccgata
     5581 tctttcatct gtgaaccgca atgttcacaa aattgttctt ccaaaggaag gagatgttct
     5641 ttctctgtta taggtaagtg tttgattttg tctgctttcg ttcctacaac tcgttgatgt
     5701 ccttttactg gaataatggt ggcagctgct tctggaacac ttacagaaat ttcttcatca
     5761 ctaaaaagat tcaattgccc attcgtttcc tcaaagagag tctctttaga gcgtccaaat
     5821 aatttgttgg ttaaataatt gaccttttca ttcaatagtt ggatttcagt tgtaagattc
     5881 tgaatggttt gttgttgtgc ttgattggtt ttatgggctt cttgaagcaa ctgaagcaac
     5941 gtatctgtag tttccaaaat ttttcaccac cttgaatcga actgattttg ttacagaata
     6001 ccacaagaaa cgcacaagaa ataggggttc aacacatttt ttgtgttaac cccccagctt
     6061 aaaaaactcc tttttttcct ggcaaaatag ctcgaggttg atcgatactt aatccttcta
     6121 acaaccatcg aacctgttgg ttcgttaagg ttcgaacttc gtctttcgtc cgaggccatt
     6181 ttaacttgcc gttttctatc cgcttataac agagaagaaa accatcaccg tcccaataga
     6241 gaagtttgaa acggtcttga cggttcccgc aaaataaaaa aacggcatct tcgtaaagct
     6301 cgagttgata ctcttgttga ataagactgg caagaccatc aattcctttt cgcaaatctg
     6361 tttttccgca cacaatataa atatgtttgg tttgaagtaa atcataaagc attggacaac
     6421 agttctttca aaatagcttg aagaatataa ttgttaatac cgttatagat caaaacttca
     6481 gaaccattct ctcttttgat gcgcatcgct aacttggcag ccgcgggttt acctttttct
     6541 ggtgcactct tttcgtttcc taattggaca ggaacaatct catttaattt atacatagaa
     6601 aacctccaga cttcttgata gctcaagtat atcttgaggt gcatttgtta gtaaggtact
     6661 gttctatggg gcgcttacgt tgaaaaacgt tgatttatcg gcattcccca tccatattat
     6721 atttacgtct tattcccact caatatgagt agcctcgtaa agaagctatt atatcagtgc
     6781 tttttcagcc tattcctcat tttggtaggg aaatggtagg gttagatttt tactgcttga
     6841 tttccattaa agtttgcaac tttagatttt gttgcacagg taaatttaat gtttcctgtc
     6901 atttgttctg caattccttc atcattacgg ctccatagat gagcgtagtg ctttaaagtg
     6961 atttcagggc tagaatggcc taaccgttgc gacacgacca gaatgtctgc gttaaactca
     7021 ttgattaggt agctaacatg agaatgacgt aatccttttc cttgtattag gggtacctta
     7081 gctagtttgg cataacgctg aataattcgt tgaacagttg aacgatagag tgggaggtca
     7141 gtgtagctaa gaataaattg ttctacacca tgattttttt gaataacttt ccactttttt
     7201 agcacttcaa ttgtatcatc gtccagagaa atgatccgca agccgctttc agtttttgtg
     7261 taaggtttcc gaataaaatc tttttgattt gtcatttcca aggtgtgatg aaccctcaac
     7321 tttttacgtt tgaagtccac gtcattccaa ttaagtgcta atccttcgct tacacgaaca
     7381 ccagtcatgt aatatagcca gatcattaaa aaacacatat gttcgtagaa atcatcttta
     7441 taaatttttg ccaaaacttc ttcaaattct tcttttgtcc agtaaggaac gattgatttt
     7501 ccttttggaa ttgcttttgt tcttttggaa atattctcat tcagaaattg cagggtgacc
     7561 gcatagtcta atgatttacg aaacataccg tacataagtg aactgtatgc ttgtgagtat
     7621 ccgctatcgt tcaataacca aattcgataa ttttcacaat ctcgcacatt gatttcacga
     7681 agcttcttat caccgaaaaa ttcagtaatc tgaatcaatc cacactcacg agaactccaa
     7741 gtactttttt gtacgctagc tttgtagtgt ggaacgtaag actgctccat aaattctttg
     7801 taagttaaat cataattcgc aaatccattt acaaccatgt attcattttt tatacgcatg
     7861 acttctttgt gtgcttcacg agctgatgag aatcgttttc ctagagaatc ttttgttcct
     7921 ttcttctgga tcctctttcc agtcacttga tctgttccta actcaactag ataagaaaag
     7981 cgaccatttt tatcttgata cacacaaggg tattttgttt tcatagttat tcctcctgtg
     8041 aaagaggaac acctataatt tcttcaatta cttgtcgggg tactacccca actcttttat
     8101 tattataata tggatatccc tgatttacca ttcttgtttt tgcttgtcgg attattgtgg
     8161 tagcttgatg tgttttaaat cctagagcct ctaaatcttt tcgacaaatt gtatttttca
     8221 ttgtataaac tcactttcaa agaaaacact atcattgggt aatagtgtta tttctttatt
     8281 tttgtataat gaacggtact ttcttgaaaa tccatcactt gttcaggact tcccaactca
     8341 tttaaagcta aagcattgta aaaaaatgga cgtctaacta tatattcgct tgtatatagt
     8401 atatcttgat tcgtggaatc atagggtttc tttaccatct ttttggtgac agttggtagt
     8461 ttgagattcc gggacttgaa aaaagctttt ttcttatgct ctttcaattc cagctcttta
     8521 tcgaaatatt tgctgatata gcgcccgcga ttttcgatac tgtctacatc gatcttgttg
     8581 atcttaacga aaccctgacc ccaaatcgta gttaaccggt taaatgagat aaaggggaaa
     8641 tcaaacaaga taatatggta gtggattgca ccgcgttgct gcttttccca cgtggcgaga
     8701 tactttaaat ttgctttttt agttttat
//
LOCUS       DACCZY010000118         8663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCZY010000118 DACCZY010000000
VERSION     DACCZY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.122"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            63..965
                     /gene="gnd"
                     /locus_tag="IVZ67_002370"
     CDS             63..965
                     /gene="gnd"
                     /locus_tag="IVZ67_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8166406.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            967..1968
                     /locus_tag="IVZ67_002371"
     CDS             967..1968
                     /locus_tag="IVZ67_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8166407.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1965..2249
                     /locus_tag="IVZ67_002372"
     CDS             1965..2249
                     /locus_tag="IVZ67_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8166408.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2282..3079
                     /locus_tag="IVZ67_002373"
     CDS             2282..3079
                     /locus_tag="IVZ67_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8166409.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3093..3926
                     /locus_tag="IVZ67_002374"
     CDS             3093..3926
                     /locus_tag="IVZ67_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166410.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3944..4801
                     /locus_tag="IVZ67_002375"
     CDS             3944..4801
                     /locus_tag="IVZ67_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8166411.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4823..5332
                     /locus_tag="IVZ67_002376"
     CDS             4823..5332
                     /locus_tag="IVZ67_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166412.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5369..5770
                     /locus_tag="IVZ67_002377"
     CDS             5369..5770
                     /locus_tag="IVZ67_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166413.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5882..6235
                     /locus_tag="IVZ67_002378"
     CDS             5882..6235
                     /locus_tag="IVZ67_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166414.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6269..6712
                     /locus_tag="IVZ67_002379"
     CDS             6269..6712
                     /locus_tag="IVZ67_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8166415.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6729..7814
                     /gene="uxuA"
                     /locus_tag="IVZ67_002380"
     CDS             6729..7814
                     /gene="uxuA"
                     /locus_tag="IVZ67_002380"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8166416.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7807..8520
                     /locus_tag="IVZ67_002381"
     CDS             7807..8520
                     /locus_tag="IVZ67_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8166417.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         2901 a         1361 c         1964 g         2437 t
ORIGIN      
        1 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
       61 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
      121 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
      181 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
      241 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
      301 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
      361 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
      421 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
      481 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
      541 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
      601 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
      661 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
      721 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
      781 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
      841 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
      901 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
      961 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
     1021 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
     1081 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
     1141 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
     1201 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
     1261 gtatctatca tacctgcttt aattgaacga aatgctgtag cagaaaataa tataactaat
     1321 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
     1381 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
     1441 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
     1501 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
     1561 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
     1621 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
     1681 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
     1741 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
     1801 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
     1861 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
     1921 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
     1981 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
     2041 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
     2101 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
     2161 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
     2221 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
     2281 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
     2341 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
     2401 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
     2461 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
     2521 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
     2581 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
     2641 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
     2701 tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat
     2761 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
     2821 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
     2881 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
     2941 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
     3001 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
     3061 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
     3121 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
     3181 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
     3241 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
     3301 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
     3361 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
     3421 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
     3481 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
     3541 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
     3601 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
     3661 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
     3721 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
     3781 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
     3841 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
     3901 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
     3961 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
     4021 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
     4081 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
     4141 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
     4201 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
     4261 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
     4321 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
     4381 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
     4441 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
     4501 aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
     4561 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
     4621 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
     4681 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
     4741 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
     4801 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
     4861 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
     4921 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
     4981 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
     5041 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
     5101 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
     5161 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
     5221 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
     5281 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
     5341 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
     5401 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
     5461 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
     5521 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
     5581 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
     5641 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
     5701 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
     5761 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
     5821 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
     5881 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
     5941 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
     6001 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
     6061 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
     6121 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
     6181 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
     6241 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
     6301 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
     6361 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
     6421 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
     6481 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
     6541 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
     6601 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
     6661 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
     6721 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
     6781 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
     6841 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
     6901 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
     6961 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
     7021 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
     7081 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
     7141 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
     7201 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
     7261 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
     7321 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
     7381 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
     7441 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
     7501 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
     7561 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
     7621 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
     7681 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
     7741 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
     7801 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
     7861 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
     7921 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
     7981 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
     8041 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
     8101 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
     8161 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
     8221 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
     8281 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
     8341 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
     8401 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
     8461 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
     8521 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
     8581 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
     8641 caaacacaaa acgcatctac tgt
//
LOCUS       DACCZY010000119         8646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCZY010000119 DACCZY010000000
VERSION     DACCZY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.123"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(244..2625)
                     /locus_tag="IVZ67_002382"
     CDS             complement(244..2625)
                     /locus_tag="IVZ67_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8166418.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2615..3262)
                     /gene="recU"
                     /locus_tag="IVZ67_002383"
     CDS             complement(2615..3262)
                     /gene="recU"
                     /locus_tag="IVZ67_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8166419.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3326..3865
                     /locus_tag="IVZ67_002384"
     CDS             3326..3865
                     /locus_tag="IVZ67_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8166420.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3955..4374
                     /gene="gpsB"
                     /locus_tag="IVZ67_002385"
     CDS             3955..4374
                     /gene="gpsB"
                     /locus_tag="IVZ67_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8166421.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4396..4763
                     /gene="rnpB"
                     /locus_tag="IVZ67_002386"
     ncRNA           4396..4763
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVZ67_002386"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4972..6138
                     /locus_tag="IVZ67_002387"
     CDS             4972..6138
                     /locus_tag="IVZ67_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8166422.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6184..7680
                     /locus_tag="IVZ67_002388"
     CDS             6184..7680
                     /locus_tag="IVZ67_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8166423.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7761..8444
                     /locus_tag="IVZ67_002389"
     CDS             7761..8444
                     /locus_tag="IVZ67_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8166424.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2718 a         1569 c         1836 g         2523 t
ORIGIN      
        1 aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg gataaagtat
       61 atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat
      121 gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt
      181 tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt
      241 ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc
      301 cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga
      361 gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc
      421 tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct
      481 ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat
      541 agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc
      601 accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga
      661 aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac
      721 aggcgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc
      781 cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc
      841 tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt
      901 accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg
      961 agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat
     1021 tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc
     1081 tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga
     1141 gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc
     1201 cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta
     1261 aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt
     1321 cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa
     1381 atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga
     1441 agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat
     1501 ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc
     1561 gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc
     1621 cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc
     1681 ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga
     1741 ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat catattctgt
     1801 ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc
     1861 ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa
     1921 tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt
     1981 ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc
     2041 ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt
     2101 cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa
     2161 agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg
     2221 aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc
     2281 ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc
     2341 ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt
     2401 atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa
     2461 gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg
     2521 tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt
     2581 aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt
     2641 caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa
     2701 taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc
     2761 ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc
     2821 gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt
     2881 tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg
     2941 tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg
     3001 aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt
     3061 tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat
     3121 atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga
     3181 tctgatttct tttttcttga attttttcga cgggagaggc attcttataa taaggttggc
     3241 catttggata gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag
     3301 cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta
     3361 tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa
     3421 tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag
     3481 tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata
     3541 tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga
     3601 agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag
     3661 ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca
     3721 tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt
     3781 tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga
     3841 cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta
     3901 tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg
     3961 aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc
     4021 tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta tgaaacttac
     4081 agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa
     4141 ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc
     4201 gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt
     4261 ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac
     4321 tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg
     4381 attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc
     4441 catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg
     4501 tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt
     4561 gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc
     4621 tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa
     4681 aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa
     4741 agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt
     4801 aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat
     4861 cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt
     4921 ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa
     4981 aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa
     5041 gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga
     5101 acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc
     5161 gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg
     5221 ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag
     5281 tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt
     5341 ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt
     5401 cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca
     5461 agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg
     5521 gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta
     5581 tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga
     5641 ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat
     5701 gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca
     5761 gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc
     5821 tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg
     5881 gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa
     5941 ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg
     6001 actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg
     6061 tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa
     6121 aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag
     6181 ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct
     6241 ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga
     6301 agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc
     6361 aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc
     6421 gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg
     6481 agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca
     6541 caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg
     6601 ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa
     6661 cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt
     6721 tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg
     6781 agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat
     6841 gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg
     6901 aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga
     6961 gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat
     7021 acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag
     7081 atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt
     7141 gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca
     7201 aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt
     7261 cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa
     7321 atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc
     7381 atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta
     7441 gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat
     7501 gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa
     7561 tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat
     7621 ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa
     7681 aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac
     7741 ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc
     7801 aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag
     7861 ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt
     7921 acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc
     7981 ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca
     8041 ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag
     8101 gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta
     8161 ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa
     8221 cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa
     8281 aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa
     8341 gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg
     8401 aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga
     8461 agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt
     8521 ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa
     8581 tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg
     8641 tttatt
//
LOCUS       DACCZY010000120         8030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCZY010000120 DACCZY010000000
VERSION     DACCZY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.124"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            70..771
                     /locus_tag="IVZ67_002390"
     CDS             70..771
                     /locus_tag="IVZ67_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8166425.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="IVZ67_002391"
     CDS             complement(1380..2396)
                     /gene="tsaD"
                     /locus_tag="IVZ67_002391"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8166426.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="IVZ67_002392"
     CDS             complement(2393..2857)
                     /gene="rimI"
                     /locus_tag="IVZ67_002392"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8166427.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="IVZ67_002393"
     CDS             complement(2864..3406)
                     /gene="rimI"
                     /locus_tag="IVZ67_002393"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8166428.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3390..4214)
                     /gene="tsaB"
                     /locus_tag="IVZ67_002394"
     CDS             complement(3390..4214)
                     /gene="tsaB"
                     /locus_tag="IVZ67_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8166429.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4303..5283)
                     /locus_tag="IVZ67_002395"
     CDS             complement(4303..5283)
                     /locus_tag="IVZ67_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8166430.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5307..6791)
                     /gene="galT"
                     /locus_tag="IVZ67_002396"
     CDS             complement(5307..6791)
                     /gene="galT"
                     /locus_tag="IVZ67_002396"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8166431.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6803..7792)
                     /gene="galE"
                     /locus_tag="IVZ67_002397"
     CDS             complement(6803..7792)
                     /gene="galE"
                     /locus_tag="IVZ67_002397"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8166432.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2273 a         1715 c         1412 g         2630 t
ORIGIN      
        1 ttaaaacttt acaaattctt taaaaaaagt gacaataaat atagtgaaca ataaggagag
       61 gactttttaa tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct
      121 gattttgaac atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt
      181 tcttataaaa aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc
      241 ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat
      301 gactatgtga atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac
      361 acttatacag ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat
      421 gaaaaattag cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa
      481 gtagttgaaa tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg
      541 gtaaaacaaa atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat
      601 atcaccattc cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt
      661 gaaactgctg gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa
      721 aagcgtttta ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa
      781 aaataagcag tgaccctgat aaatatcagg gtcactgctt attttcctcc ctaaaacttc
      841 tgaaagatcc ggcagtttta gatcactgga caagactcaa agattttcta tgtgtgtttc
      901 agttatgtaa tattcactgt ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt
      961 gtcctcatcc gatctagatt attcgatatt cctcgacaat aaacgattac tcagtgaggg
     1021 caatcagagg aacggataat gataaataag ctgtaaaaaa tagcaaaata acaataatga
     1081 ttaaaagtat gtaaaaaata gggctttgac aaaaacgtgt cacagcccta tttcgattaa
     1141 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1201 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1261 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1321 agccgttttt cctgggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     1381 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1441 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1501 gggggaatca ctagttctac ctcagggagt ttagctgcca aagcatcctg caatccttca
     1561 cgcaaccctt gattggccgc tacacctcca gctataatca gttgcttgac tggataattt
     1621 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1681 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1741 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgc
     1801 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1861 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     1921 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     1981 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     2041 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2101 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2161 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2221 gtgatttgtt ctacgtgatg gcggctagct acttcaggta ctacgccacc aaaacgttta
     2281 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2341 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2401 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2461 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2521 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2581 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2641 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2701 cttcagctaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2761 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2821 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     2881 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     2941 ttgcttgaaa ccccaatttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     3001 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3061 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3121 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3181 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3241 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3301 attctcttag tacgtaacga cgatcgattt gttcgactgt tttttctgta taaatcattt
     3361 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3421 ggtgtgtgcg tctccaacca tttttcttct gcttctactc gttttaaata gcgaggaaga
     3481 aaagcttgtt tctctgcaac tggttcaact aaagcaccta attgagccac agttacacca
     3541 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3601 tcagagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3661 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacagt ttctaatcta
     3721 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3781 acaataatat tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3841 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     3901 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     3961 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     4021 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4081 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4141 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4201 gtacaataat acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa
     4261 aagctgcggt atcactccat tttcccacag cttttccatc ttttatttcg ttgattctcg
     4321 aataatcaac tctgtttcca caataatctt tcttgaaacc ggtgctttgg aattacttaa
     4381 ttgaagaatc gtctccactg ctagctctcc catccattcg gtataaactt ttacagtaga
     4441 aagcgagggc gttacatatt tcgctacact gacatcattg aagcccatca cagaaatatc
     4501 ttctggtacc actaaacctt tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc
     4561 attagctgca aaaagcgcat caggaacatg cggattcgtt tttaaatatt gcttcactgc
     4621 ttgatatcct gactcgacag aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt
     4681 ttctgccaaa acttgttgaa atgccataat tctgggatct tttagccgct gatggttttt
     4741 cttcgtatat tctgttccag caagcatagc gatatgttgc gctttatggg agacaaagtg
     4801 ttcaatgact ttttgaacac ttgacttaaa gtcgacaact agagagtcga ttccttgaga
     4861 agtgccgtca gaatctacca aaagcaaggc attttgggat cctttcattt ctaataactg
     4921 agcttcgtcg aattttccta gtgcgatcgt tccatctgcc ggaagatcac taatggaatc
     4981 ccacgtttct ttcaataaag taacgtttgc ttcttctgcc tttttttcga ttcctaggcg
     5041 aatagataaa taataaagat cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg
     5101 aagcgtttgc ttagtatttg ctgcattttt acgatgtttc gtataattta agcgttctgc
     5161 tgcttcaaaa attttttgct ttgtggctaa gccaacggaa agatcaggat catagttgag
     5221 aactcttgaa acagtagcag gagaaactcc tgccaactgc gcaatgtctt taattgttgc
     5281 catttgtctc tcctcctttt ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc
     5341 tgtccttttt catctcgctt gaatactcca gcgtcttcca gtacacgtgc aaaaacctga
     5401 cctacagatt tttgtacgac atcggtgacc gtatctttcg tcaaatcagg atgttctttt
     5461 tttaattgtt ctgcccacgg acgatgatat tcagccatct ggttttcttc tcctaataag
     5521 aaatgctcta cttctactag ttctggtttc aaacgaggtg gtaaaacagc aagccccatt
     5581 acttcgatca acccaatgtt ctcctttttg atatgttgga catctttatg tggatgaaaa
     5641 atcccatcag gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag
     5701 taattgtccc ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc atctgctgaa
     5761 aaagctctga tagatactgt ttcatccgaa tatgctttcc atttttccaa tactgagtta
     5821 gctgcttcaa taagcaagtt tttctctgtt gattgcaaac ggatcacaga catcggccat
     5881 ttcacgactc ctgcgatgac ttgcggataa tcttctagtt tgataagttt ttctatcggt
     5941 gcttttgcca taggaaattc atgacgtcct gcctgataat gatcatgaga aagaatcgat
     6001 cctccaacaa ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa gacttcagta
     6061 atccgaagca gtctagcaaa tgtttccttt gtgattttca tcgggcggtg ttgttctgat
     6121 aaaatgatcg aatgttcatt gtaataagca taaggggaat attggaatcc ccagctttca
     6181 ccgtctagat tcattcgaat aatccggtga ttggtgcggg ctggatagtt caagcgacct
     6241 ctatatcctt cgttttccat gcaaagcatg caggtaggat agtctacttt taccgcctcg
     6301 cgttctgctg cgatttgttt tggatctttt tctggcttag ataaattgat tgtgatttcc
     6361 agttcaccat actccgactc tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg
     6421 atgtagtcat ttttcttact taaattaaag aaatactcag ttgcttcctc tgggcttttc
     6481 gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag aaaatccatt
     6541 aattgcgctt caaaaatctc ttgttctgct ggcaattctg aaagaacacc attttctctt
     6601 gccttagcga caagctgatc caataagtct acagattctt ccttaactgg tcttacttca
     6661 atgctttcta gagcatcttc tcctaccatc cctaaaagac gattctgtaa atataagcgg
     6721 tccatttcca tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga
     6781 ctgattgaca tatctcattc ccctattctt cataaccatt tggatggctg acatgccaat
     6841 cccatgctgt tttgataatc gctttgatat ctgtgtattc aggtttccag cccagaatct
     6901 ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc tccagctctt cttggtgcta
     6961 tttctgctgg aatttctttt cctgtcactt ctctagctgc ttctagcatt tccttgactg
     7021 agtatccttt gctgctgcct aaattgaaga agttgctttc atttccttct ttcaagtatt
     7081 ccaaagctaa aatatgtgca gcaatcaaat cttctacttg aacataatct ctgatacatg
     7141 ttccatctgg tgtatcataa tcgtctccat aaacagccaa ggcttttctt tgaccaagag
     7201 ctacttgtaa aatgattggg acaagatgcg tttctggtgt atgatcttct ccgatagatg
     7261 catctgcttt tgctccggct acattgaagt agcgcaaagc gacatagcgc atgccatatg
     7321 cttggtcaca ccacttcatc attttttcca tcatcaattt actttcacca tacggatttt
     7381 taggattagc tggtgtatct tctgtgattg gagactcttt cggctctcca taagttgctg
     7441 cagtagaaga gaatacgatt ttattcacgt tgaactcgtg catgacttca agtaagattt
     7501 gcataccgta tacgttgttg ttgaaataca tcaatggttt ttcgacagat tctcctacta
     7561 aagagcttgc agcaaaatgg attactcctt caatcggttc cttttcaaat acggaacgta
     7621 gaaattcttt atcccggatg tctccttcat aaaaatgagc atctggatgt actgcttgtt
     7681 tgtgacctgt gagcaaatta tctaccacag ctacttgata ccccttttga actagctggt
     7741 ctacagcgtg cgaaccgata taaccggctc cgcctagtac taaaattgtc ataaaaaaca
     7801 ctcctttgat tagtaaaatt gtgtttgttg attctatata atttaaaaaa cgaagtttac
     7861 ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc atatggtaaa tttataataa
     7921 aacatttagt aaacattttc aagggttttt tgcaaaggtt tacaaacaaa cgtaaaacta
     7981 gccttttttt ctcttttgtt ccataataga tataacaaat aataaatgag
//
LOCUS       DACCZY010000121         7736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCZY010000121 DACCZY010000000
VERSION     DACCZY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.125"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..210)
                     /locus_tag="IVZ67_002398"
     CDS             complement(<1..210)
                     /locus_tag="IVZ67_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8166433.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLE"
     gene            complement(207..725)
                     /gene="msrA"
                     /locus_tag="IVZ67_002399"
     CDS             complement(207..725)
                     /gene="msrA"
                     /locus_tag="IVZ67_002399"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8166434.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(745..1458)
                     /locus_tag="IVZ67_002400"
     CDS             complement(745..1458)
                     /locus_tag="IVZ67_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8166435.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1461..2357)
                     /locus_tag="IVZ67_002401"
     CDS             complement(1461..2357)
                     /locus_tag="IVZ67_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8166436.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2515..3357)
                     /locus_tag="IVZ67_002402"
     CDS             complement(2515..3357)
                     /locus_tag="IVZ67_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8166437.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3489..4142
                     /locus_tag="IVZ67_002403"
     CDS             3489..4142
                     /locus_tag="IVZ67_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8166438.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4205..4732)
                     /locus_tag="IVZ67_002404"
     CDS             complement(4205..4732)
                     /locus_tag="IVZ67_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8166439.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4752..5699)
                     /locus_tag="IVZ67_002405"
     CDS             complement(4752..5699)
                     /locus_tag="IVZ67_002405"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8166440.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5711..7597)
                     /locus_tag="IVZ67_002406"
     CDS             complement(5711..7597)
                     /locus_tag="IVZ67_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8166441.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         2005 a         1582 c         1359 g         2790 t
ORIGIN      
        1 ttctaaatat ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt
       61 cagttccaga taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc
      121 tttaccggct tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt
      181 cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag
      241 gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga
      301 tggtcttctt ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt
      361 tcgaaacgtc cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct
      421 tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa
      481 gcgtcagttg gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat
      541 gttggatcat agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt
      601 tcataagtcg gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca
      661 ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt
      721 tccattatga ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt
      781 gctttcagta ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt
      841 atcttttggc aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc
      901 tttaatatac atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc
      961 atctgtattg acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac
     1021 atctttgatt ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt
     1081 tccgttttcc atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac
     1141 ttcgaaagta ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact
     1201 atctttttga tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga
     1261 attgatcgtt aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc
     1321 tggttcccga ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc
     1381 aagaaggata aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac
     1441 gtcagatctt gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc
     1501 tcgatcagct gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg
     1561 aaatgatctt cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta
     1621 gttgtttgat ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc
     1681 aaatcattga ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct
     1741 ttgttccaat tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat
     1801 ggattgtaga tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc
     1861 aatagttctg tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt
     1921 aacccgaata aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg
     1981 ataaaatcag ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag
     2041 atctggtcac ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta
     2101 taacggtctt ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct
     2161 cctactcctt cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt
     2221 ggatcttctc tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca
     2281 gctaaacctg catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt
     2341 cgtttcgtta atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa
     2401 ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa
     2461 gaaaaagaga ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc
     2521 tacataatac atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt
     2581 atgcagtaca gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg
     2641 cgccaattct tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt
     2701 cagttcattt ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt
     2761 tacagggatt aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga
     2821 cagcaaacca gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat
     2881 tccgatgtat aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac
     2941 tgttttacct tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc
     3001 agtggtatca ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc
     3061 tgcttctact gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc
     3121 ttctcctagc tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg
     3181 cagatttttt gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc
     3241 gggatatact gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc
     3301 gcgtacattt ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat
     3361 tttctctctc tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat
     3421 ttctattttt agtataacag acaaataatg atttttcatt aattacacct agtaaaggag
     3481 ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta
     3541 atgcagtcac ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag
     3601 taaaaggtgc ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga
     3661 tgatgattct tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa
     3721 aaaaggtgtt ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata
     3781 ctcctttttg tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct
     3841 ggttgtttgc gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag
     3901 tatcaaaagt atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg
     3961 ttcctcttta tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt
     4021 cttacacgat aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt
     4081 ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt
     4141 aataaaaaaa ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc
     4201 ttttttatat cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg
     4261 tttcaaattg atgcggataa cgattttttt catccatttc tccttgacta gtattgatca
     4321 gtgaccaatc agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga
     4381 tgactgtacg gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc
     4441 cagcgatgaa aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat
     4501 gcatgacatg cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac
     4561 gattcggcaa cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat
     4621 ttgcttctgt catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt
     4681 cttcttttcc aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat
     4741 cctttctgat tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct
     4801 aattggatat cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa
     4861 gtcggaaatg cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata
     4921 taaagatgag catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt
     4981 gcaattaggt gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca
     5041 tccccactgc gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac
     5101 atcgtatgac aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg
     5161 atcaatcttc tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg
     5221 ataaagccac cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga
     5281 tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc
     5341 tgctttttat attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc
     5401 atatctggtc cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa
     5461 atatggtttt tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc
     5521 aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt
     5581 tgcagatcaa accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct
     5641 tctttacgat gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc
     5701 ctttttcctc ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt
     5761 caagttgtgc ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat
     5821 ctccttgatg attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct
     5881 catcttcaat cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag
     5941 gttccttgct ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct
     6001 tcagtaaata actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata
     6061 ctaataactt atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag
     6121 cacctggaaa acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat
     6181 tggtcggttc atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata
     6241 accggcgttt ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa
     6301 ataaaaatcg ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt
     6361 cttcagcagc ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt
     6421 cttgtgtata atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg
     6481 gaattctgcc agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa
     6541 tacctagtct ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat
     6601 ttatacggta actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta
     6661 ggttcagctc taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga
     6721 aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag
     6781 ctaattcctg tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac
     6841 gttctgcttt ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa
     6901 atgaaagttc gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca
     6961 tcaatagtgc gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat
     7021 ctaaatggtt tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg
     7081 ccagactaac tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac
     7141 tgattcctaa tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt
     7201 tattcatttg ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt
     7261 cccctagttc gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc
     7321 cttgaaagac tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat
     7381 aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga
     7441 taccggcaat aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga
     7501 tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag
     7561 tttttttgag gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt
     7621 gtatcataaa aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct
     7681 caaaaaaaga acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaa
//
LOCUS       DACCZY010000122         7732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCZY010000122 DACCZY010000000
VERSION     DACCZY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.126"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..107
                     /locus_tag="IVZ67_002407"
     CDS             <1..107
                     /locus_tag="IVZ67_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP8166442.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="IVZ67_002408"
     CDS             complement(176..904)
                     /locus_tag="IVZ67_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166443.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="IVZ67_002409"
     CDS             complement(917..2197)
                     /locus_tag="IVZ67_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8166444.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="IVZ67_002410"
     CDS             complement(2338..2940)
                     /locus_tag="IVZ67_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8166445.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="IVZ67_002411"
     CDS             3185..3391
                     /locus_tag="IVZ67_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166446.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="IVZ67_002412"
     CDS             3703..4422
                     /locus_tag="IVZ67_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8166447.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="IVZ67_002413"
     CDS             complement(4503..5186)
                     /locus_tag="IVZ67_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8166448.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="IVZ67_002414"
     CDS             5405..7591
                     /locus_tag="IVZ67_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8166449.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2775 a         1301 c         1461 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga gg
//
LOCUS       DACCZY010000123         7639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCZY010000123 DACCZY010000000
VERSION     DACCZY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.127"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            17..2344
                     /locus_tag="IVZ67_002415"
     CDS             17..2344
                     /locus_tag="IVZ67_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8166450.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="IVZ67_002416"
     CDS             2470..2799
                     /locus_tag="IVZ67_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8166451.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="IVZ67_002417"
     CDS             2839..3843
                     /locus_tag="IVZ67_002417"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8166452.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="IVZ67_002418"
     CDS             3840..4286
                     /locus_tag="IVZ67_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8166453.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="IVZ67_002419"
     CDS             4436..5251
                     /locus_tag="IVZ67_002419"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8166454.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="IVZ67_002420"
     CDS             5457..6629
                     /locus_tag="IVZ67_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8166455.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="IVZ67_002421"
     CDS             complement(6839..7285)
                     /locus_tag="IVZ67_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166456.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="IVZ67_002422"
     CDS             complement(7282..7476)
                     /locus_tag="IVZ67_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166457.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2572 a         1443 c         1523 g         2101 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggattttt
//
LOCUS       DACCZY010000124         7512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCZY010000124 DACCZY010000000
VERSION     DACCZY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.128"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            374..1366
                     /locus_tag="IVZ67_002423"
     CDS             374..1366
                     /locus_tag="IVZ67_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8166458.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1382..2266
                     /locus_tag="IVZ67_002424"
     CDS             1382..2266
                     /locus_tag="IVZ67_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8166459.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2315..3922
                     /locus_tag="IVZ67_002425"
     CDS             2315..3922
                     /locus_tag="IVZ67_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8166460.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3923..5407
                     /locus_tag="IVZ67_002426"
     CDS             3923..5407
                     /locus_tag="IVZ67_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8166461.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5584..6576
                     /locus_tag="IVZ67_002427"
     CDS             5584..6576
                     /locus_tag="IVZ67_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166462.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6628..7497
                     /locus_tag="IVZ67_002428"
     CDS             6628..7497
                     /locus_tag="IVZ67_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8166463.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2397 a         1200 c         1654 g         2261 t
ORIGIN      
        1 agcttctttt gctaaataga ttaaacttac tggtgagtgg tcttgaacat acttttaaat
       61 cacattttac tgagttttat tggattttat aattcattgg aaacctcgta cttaatctgt
      121 ttttatctat cgactagaat ctgtatatat tccagctatc aatgaggccg cagaaaaggg
      181 tgcttaaaaa taatctgttg tttaaaatat tattatcaga atacttagat acaaaattct
      241 ggcacaaagt aaaaaaaata ttgacttaaa aaaatggata tggtaatcta taaacgaaaa
      301 tagtggtaac gtttccaaaa tgcatcttcg ttaaattttt atttttttaa acgaaaatgg
      361 aaacgtttct aaaatgtatt gggaggagat tgaattggaa aagacattgg gaccaggtgg
      421 tgagataagg gcggtacaac gggataaaaa aaatatttcg aagtgggaaa aattaaagaa
      481 acagttgcgg cgtgataagt ggatgtactt gttgctctta ccaggtatca tcatgacgtt
      541 tatctttaag tatgtgccaa tgtacggtgc tgtgattgca tttaaagact ataatcctat
      601 gctaggaatt ttggaaagtc cttgggtagg actagatcac tttagggaat ttatcagttc
      661 accaaatttt acgcttttgc tgagcaacac attaaaaatt agtatttatg gtttattgtt
      721 aggttttttt ccaccaatta ttttagcact gagcttgaat caaattatca ataaaaaaat
      781 aaaacagaaa atccagttga ttctatatgc gccgaatttt atctcagtag ttattattgt
      841 ggggatgctg tttttgttcc tatccgcaaa tggacctatt aatgggttag tagaaaaggt
      901 tacggggcaa ccaattatgt tcatgtctaa tccagcttat ttcagaacga tttatatttt
      961 atcgggaatt tggcaaggaa tcggctggtc atccattctt tacaccgcaa cgctggcggg
     1021 agtcagtacc gatttatatg aggcagcaaa tatcgatggg gcaaatattt tacaaaagat
     1081 ttggcatatc gatttgcctg cattgaggcc tgtgatggtg atcagcttca tcttggccgc
     1141 cggcggtatt atgaatgtag gatatgaaaa agcctacttg atgcaaactt caatgaacat
     1201 ccctgcctca gaaatcattt ccacttatgt atacaaagtc ggcttgcaat caggtgatta
     1261 tgcctactct gctgctgttg gtctgttcaa tatgattatt aatgtcgttt tattactgat
     1321 tgtcaacaat ataaccaagc ggctaaacga tggtcaaggc ttgtaatgaa aggagaaaat
     1381 tatgaatagt gctttatcaa aaatcgaccg tcaaatctta tggttgaatc gagtgattgt
     1441 aactttttta gtcgtcatca ccattgttcc gctgatttat gtactggtag cttcttttat
     1501 ggacccagct actttgatca ataaaggtat aagcttcaat ccgaaagatt ggacaataca
     1561 gggatatcaa cgggtatttg cagatgattc cattttacgc ggttttgtaa attcgttgtt
     1621 ttactctttt agcttcagct tattgactgt atttattacg atgatcactg cgtatccttt
     1681 gtctcgacct aacttggcgg ggaaaaagat tattatgact ttttttgtca ttacaatgtt
     1741 cgtgggtggt ggcttgatac ctacctattt attggtaaag aacttgggca tgttagatag
     1801 tgtatgggcg attatcgtac caggtgctat aaatgtgtgg aatattatct tagcccgtac
     1861 ctattttagt gctttgccaa aagaactttc tgaagctgct actatcgatg gcgccaatga
     1921 cttgcaaata ctgttcaaag tcatgcttcc attagcgaaa ccaattatgt tcgtattgtt
     1981 tttatatgcc tttgttgggc aatggaactc atactttgat gccatgattt acatcaaaga
     2041 tcccgactta cagccgttgc aactcgtatt aagaaatatt ttgatccaaa accaaccttc
     2101 acaagacatg gtgggagcca atactgccat ggcagaaatg aaacaaattg ctgagttgat
     2161 caaatacgca acgatcgtca tttctagtct gccattaatc ataatgtatc ctttcttcca
     2221 aaaatatttt gataaaggtg ttttagttgg ttctgttaaa gggtaagtat tgttcactat
     2281 ttgtatgaat aaaaattata aaggagattt taagatgaaa gcaaaatgga tgctgccaat
     2341 cgtttttact tccgtactaa ttttgggggc ttgtggaggc tcaaaagaaa agagtaaatc
     2401 aagccctgat tatgaactag aaaatgtctc ttttccattg aaagaaaaag tatctttaaa
     2461 aatgatgtct caaagtgccc ctttggctcc caatgatcct aatgacaaat tgattttcca
     2521 acgattagaa gaagagacca atctccatat tgattggaca aactacagta gtgattttgc
     2581 cgaaaaaaga aatttggata tcgctagtgg tgatttacca gatgccatct tcaacgccgc
     2641 tgcaggtgac tatgatttat taaactgggc ggaaagtggc gttattgtac cgattgaaga
     2701 tttgatcgaa gattatatgc caaatctgaa gaaaattttt gatgaaaacc ccgaataccg
     2761 acaaatgtct actgcacctg atggtcacat ttactctatg ccttggattg aagaattagg
     2821 cgaaggaaaa gagtcgattc atacagtaaa tgggatggct tggatcaaca aggagtggtt
     2881 ggataatttg ggtcttgaaa tgccgcaaac cactgatcag ttgatgactg tgttggaggc
     2941 tttcaagacg caagatccaa atggtaatgg tgaagcagat gaaattcctt ttagctttat
     3001 taacaatgga ggaaacgaag acttaaaatt tttaatgggg gcattcggtc taggtgataa
     3061 tgatgaccac ttagtcgttg ataacgatgg tcaggtacaa tttactgcta acaaagaagg
     3121 gtataaagaa gccatcaaat atttcaatga aatgaataat aaaggtctta ttgataaaga
     3181 ggctttcgaa caagattgga atacgtatgt tgccaaaggg aaagaacata agtatggcgt
     3241 gtactttact tgggataaag caaatatcac gggtgcagat gatagttatg acgtcttgcc
     3301 agtcttagcg ggaccagatg gaacgaaaca tgttactcgt acgaatggca tgggtttctc
     3361 aagagatcgt ttcgtaatta ctagtgcgaa taaaaacttg gaacttacag ctaggtggat
     3421 agataaaatg tatgaaccgt tacagtctgt ccaaaataac tggggaacct acggtgatga
     3481 agcacaggcg aatatttttg aattaaagga tggtatgtta aaacatttgc ctttagaggg
     3541 gacagcaccg ggggaactaa gacaaaaaac cgaagctgga ggaccattag cagttcttga
     3601 ttcttattac ggcacagtca ctactatgcc agatgatgcg aagtggcgtt tagatttgat
     3661 gcatgacacg tatttgccat atattacaga agacaatatc tatccacgtg tctttttaga
     3721 aaaagaagaa acagatcgat tagcaaaaat cgaaacggat atgtgggatt acatctatcg
     3781 caaaagagca gagtggatca ccggcggtaa aatcgatgaa gaatgggaag aataccttgc
     3841 tgaattggaa cgtgtaggtt tggaagaatg gattcaaata aaacaaactg gctatgataa
     3901 ttttttgaag ggagcacaat aagtgttagt agaagaaacg gcgattttgg ataaagcaaa
     3961 tcgatttatt gaaatgatgc gaggaaaagt taaccctcag tatcgacaaa actatcattt
     4021 ttccgcaccc atcgggtgga taaatgaccc caatgggttt atatattaca aagggcatta
     4081 ccatctcttc tatcaatact atccttacga tagtcgatgg gggcccatgc attgggggca
     4141 tgcgcgcagt agtgatttgg tccattggga agatctacca gtagcgttag ccccagatca
     4201 accttatgac agcggcgggt gtttctcagg aagtgcaatt gaaagagacg gaaaactata
     4261 tttattttat acaggctgtc aagaaattga tggtattagc catcagcatc aatgtttggc
     4321 ggtctctgaa gatggtattc attttagcaa atacagtggt aatcctgtgg tagcagaaaa
     4381 acagctaggt gaaaatgggc taatacaaga atttcgtgat cctaaagtaa ttgttcacaa
     4441 cgatggttac tatatggtag tagctactaa atctacacag tctactggta gaatagtgat
     4501 gtttcaatcg tcagatttgt atacttggca cttttactct gtgctgctag aagggacttc
     4561 agagcaagga ataatgtggg aatgtcctga tttattccat ttagacggac gagatgtctt
     4621 aatcatgtct ccgatacaaa tcaagaagaa cggactggaa tatcataata tcagttctac
     4681 catggcttgt attggtgagg taaactggga aacaggtcag atgatagttg ataatttcca
     4741 tgaaatggat tttggaatgg atttttatgc accacaaaca acaagagatc ctaatggtgg
     4801 acgggtgatg attgcttgga tgcagatgtg ggatcgtaca ttgcctacac atgagttagg
     4861 ccatctttgg gcaggctcta tgagtttgcc ccgagagtta agagtgaaaa aaaatcgatt
     4921 ggtacaaaaa cccatctcaa ctgtatatag tcatttagtt tatcaacaag gcagtgaaaa
     4981 tattgcagtg actgacacat cagtcgtatt tcgaaacgcg ctgtctgatt cttcatattt
     5041 acatctagtc gcagatttgg agaaagctga agtttttaca attgaattac tgcggtctaa
     5101 gacggattgt ctcaaactag cctatgacac tgctacacag gtttttgaat tggatcgtca
     5161 gaaactagga agagagctta caggggcgga agaagaccct ctgaatgtac gaaaagttga
     5221 agtacctttg attgataatc agctcgttct tgagattttt agagatacct catcaattga
     5281 agtctttatt aatgggttag aagttatgac ggctactttc tatgagctgg aaaaagctcg
     5341 agatatcttg tttcattcga tcggagaaag tcgtattcaa gcttttgaaa cagcgagtgt
     5401 gaaataaatg tttgattgct taggcgatga atcaattatt taataggttt tgaaggataa
     5461 tgtttttaga aaagaacaga tagctagatt tcccatggat ttgctattag tattttgaaa
     5521 aaattagtaa tttatgacaa aattcattat actaaacttg tgcagaaatg tgagggatca
     5581 actatgaaag caacaatgaa agatgtcgca aaattagcag gtgttggagt aggaactgtt
     5641 tcccgggtat tgaataacgg ttctgttaaa gcttctacta gagaaaaagt tgaagcagca
     5701 atgacagagt taaactatca accagatttt tatgcccggg ggttaaagat gaagcatacg
     5761 aacacggttg ctttgattct acccacggta tggcatccat tttttggcga atttgcctac
     5821 catgtagaaa cagcattaga aagacaaaac tacaagcttt atttgtgtaa ctccagtggt
     5881 aatagtgaaa aagaacaaga atatatccag atggtcagcc aaaataaagt agacggcatc
     5941 attggaatta cttattcaga tatcgatgaa tacatttcga gtaatctgcc ttttgttagt
     6001 attgaccgtt atttcactga agaggtgatt tatgtgaccg ctgacaatgc ggccggtgga
     6061 cgtttggctg caaatgaact ttatcgccga ggatgtcaga atgtagcata tatcggtggg
     6121 taccaagaaa caccgaatga aacgaagagt cgtcgaaaat attttgaaat cgagtgccgt
     6181 caactgggga tttcctataa agcgttagat atgttagaac ctttagacca tctctcagaa
     6241 cgtgtaacaa attttttgtt agagaatccc gatatcgatg ggatttttac gatcaacgat
     6301 ccaatggcat tagctgtcat tgatatgctt gaaaaaatag ggaaaaacgt accagaggat
     6361 gtccaagtta tcggatttga tggtcaaaag atgacagaag gagatcctta tctagtctca
     6421 acgattgccc aacctacaaa gcaaatggcg gaagtggcag tggcaaaatt aattgctgtc
     6481 attaatggag agacagtaga aaaacgtacc gtattgccga ttgaatttgg cgaaggtgac
     6541 acaacgaaac ctttagtgag aaacgatttt gcttagtgca aatacgtttc tttttttgaa
     6601 aatgagtgaa tgaaaggtgt agataaaatg ttatatggtg gaatagaagc cggtggaaca
     6661 aagtttgtct gtgggattgg taatgaacaa ttacaagtga tagaacggaa aagttttcct
     6721 accacaacac cagaagaaac gatggagcaa gtgtttgatt tctttgataa gtacaaagga
     6781 aatatcgcat caattggtgt aggagcattt gggcctattg atattcaaat cgaatcaaga
     6841 acttacggat atatcaccaa cacaccgaag ttagcttggc aaaattatca ttttgtagga
     6901 gctttggaaa acgccttgtc ggttcccatc tattggacta ccgatgtaaa tgctgcttgt
     6961 tatggagaat atatggcagg gtatggaaaa ggtaaaaata gtgttctgta ttacaccgtt
     7021 ggtacaggtg ttggtggtgg tgctgtagtg gatgggaaaa tagtcgcagg ttttagtcat
     7081 cctgaaatgg gacatatgct cttacaacgt cataatgaag atcaatttga aggaatctgt
     7141 ccttttcatc atgattgttt ggaagggtta gcatcaggac ctgcgattga aaaacgcttt
     7201 ggaagaaaag cccagactat ctcatttgat catccttatt ggacgattga ggcagattat
     7261 cttgctcagt gtgtgtataa cacaacgtta ctatatgcac cggaagtaat aattttaggc
     7321 gggggtgtaa tgaaacagcc gcaactactc gagttgattc gccaatcgtt taaagaaaaa
     7381 atgggttcct acgtggatac gcctactctc gaagaatatc tactaactcc aaggcttgct
     7441 gacaatgcgg gcttgattgg ctgcctagct ttagcaataa agaaactcaa tcattagaat
     7501 agtatttatt ag
//
LOCUS       DACCZY010000125         7451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCZY010000125 DACCZY010000000
VERSION     DACCZY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.129"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            143..1534
                     /locus_tag="IVZ67_002429"
     CDS             143..1534
                     /locus_tag="IVZ67_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8166464.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1607..2254)
                     /locus_tag="IVZ67_002430"
     CDS             complement(1607..2254)
                     /locus_tag="IVZ67_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8166465.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2262..2948)
                     /locus_tag="IVZ67_002431"
     CDS             complement(2262..2948)
                     /locus_tag="IVZ67_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8166466.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2954..4027)
                     /locus_tag="IVZ67_002432"
     CDS             complement(2954..4027)
                     /locus_tag="IVZ67_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8166467.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4249..4923
                     /locus_tag="IVZ67_002433"
     CDS             4249..4923
                     /locus_tag="IVZ67_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166468.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4928..6268
                     /locus_tag="IVZ67_002434"
     CDS             4928..6268
                     /locus_tag="IVZ67_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8166469.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6417..7403)
                     /locus_tag="IVZ67_002435"
     CDS             complement(6417..7403)
                     /locus_tag="IVZ67_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8166470.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2310 a         1383 c         1312 g         2446 t
ORIGIN      
        1 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
       61 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
      121 gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
      181 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
      241 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
      301 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
      361 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
      421 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
      481 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
      541 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
      601 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
      661 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
      721 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
      781 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
      841 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
      901 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
      961 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
     1021 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
     1081 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
     1141 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
     1201 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
     1261 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
     1321 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
     1381 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
     1441 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
     1501 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
     1561 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
     1621 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
     1681 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
     1741 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
     1801 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
     1861 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
     1921 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
     1981 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
     2041 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
     2101 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
     2161 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
     2221 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
     2281 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
     2341 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
     2401 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
     2461 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
     2521 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
     2581 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
     2641 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
     2701 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
     2761 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
     2821 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
     2881 gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
     2941 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
     3001 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
     3061 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
     3121 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
     3181 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
     3241 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
     3301 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
     3361 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
     3421 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
     3481 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
     3541 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
     3601 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
     3661 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
     3721 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
     3781 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
     3841 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
     3901 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
     3961 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
     4021 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
     4081 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
     4141 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
     4201 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
     4261 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
     4321 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
     4381 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
     4441 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
     4501 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
     4561 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
     4621 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
     4681 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
     4741 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
     4801 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
     4861 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
     4921 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
     4981 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
     5041 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
     5101 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
     5161 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
     5221 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
     5281 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
     5341 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
     5401 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
     5461 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
     5521 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
     5581 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
     5641 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
     5701 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
     5761 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
     5821 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
     5881 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
     5941 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
     6001 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
     6061 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
     6121 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
     6181 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
     6241 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
     6301 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
     6361 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
     6421 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
     6481 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
     6541 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
     6601 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
     6661 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
     6721 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
     6781 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
     6841 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
     6901 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
     6961 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
     7021 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
     7081 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
     7141 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
     7201 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
     7261 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
     7321 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
     7381 accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat
     7441 attgttactt a
//
LOCUS       DACCZY010000126         7390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCZY010000126 DACCZY010000000
VERSION     DACCZY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.130"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVZ67_002436"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVZ67_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8166471.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="IVZ67_002437"
     CDS             complement(1804..2697)
                     /locus_tag="IVZ67_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166472.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVZ67_002438"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVZ67_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8166473.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="IVZ67_002439"
     CDS             complement(join(3472..4509,4511..4573))
                     /gene="prfB"
                     /locus_tag="IVZ67_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8166474.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVZ67_002440"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVZ67_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8166475.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DACCZY010000127         7377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCZY010000127 DACCZY010000000
VERSION     DACCZY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.131"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            164..2773
                     /gene="clpB"
                     /locus_tag="IVZ67_002441"
     CDS             164..2773
                     /gene="clpB"
                     /locus_tag="IVZ67_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8166476.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2924..3595)
                     /locus_tag="IVZ67_002442"
     CDS             complement(2924..3595)
                     /locus_tag="IVZ67_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166477.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3588..4082)
                     /locus_tag="IVZ67_002443"
     CDS             complement(3588..4082)
                     /locus_tag="IVZ67_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8166478.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4102..5322)
                     /locus_tag="IVZ67_002444"
     CDS             complement(4102..5322)
                     /locus_tag="IVZ67_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8166479.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5469..7304
                     /gene="lepA"
                     /locus_tag="IVZ67_002445"
     CDS             5469..7304
                     /gene="lepA"
                     /locus_tag="IVZ67_002445"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8166480.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2435 a         1404 c         1508 g         2030 t
ORIGIN      
        1 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
       61 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
      121 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
      181 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
      241 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
      301 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
      361 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
      421 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
      481 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
      541 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
      601 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
      661 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
      721 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
      781 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
      841 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
      901 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
      961 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
     1021 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
     1081 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
     1141 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
     1201 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
     1261 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
     1321 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
     1381 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
     1441 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
     1501 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
     1561 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
     1621 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
     1681 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
     1741 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
     1801 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
     1861 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
     1921 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
     1981 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
     2041 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
     2101 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
     2161 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
     2221 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
     2281 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
     2341 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
     2401 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
     2461 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
     2521 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
     2581 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
     2641 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
     2701 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
     2761 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
     2821 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
     2881 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
     2941 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
     3001 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
     3061 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
     3121 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
     3181 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
     3241 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
     3301 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
     3361 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
     3421 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
     3481 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
     3541 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
     3601 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
     3661 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
     3721 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
     3781 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
     3841 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
     3901 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
     3961 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
     4021 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
     4081 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
     4141 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
     4201 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
     4261 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
     4321 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
     4381 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
     4441 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
     4501 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
     4561 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
     4621 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
     4681 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
     4741 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
     4801 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
     4861 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
     4921 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
     4981 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
     5041 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
     5101 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
     5161 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
     5221 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
     5281 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
     5341 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
     5401 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
     5461 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
     5521 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
     5581 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
     5641 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
     5701 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
     5761 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
     5821 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
     5881 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
     5941 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
     6001 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
     6061 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
     6121 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
     6181 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
     6241 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
     6301 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
     6361 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
     6421 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
     6481 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
     6541 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
     6601 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
     6661 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
     6721 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
     6781 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
     6841 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
     6901 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
     6961 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
     7021 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
     7081 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
     7141 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
     7201 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
     7261 tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa
     7321 aacagtgtaa aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtg
//
LOCUS       DACCZY010000128         7088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCZY010000128 DACCZY010000000
VERSION     DACCZY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.133"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..695
                     /locus_tag="IVZ67_002446"
     CDS             <1..695
                     /locus_tag="IVZ67_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166481.1"
                     /translation="LCIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVY
                     KGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELS
                     KSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIER
                     DSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEK
                     IYKEIIEQYFPK"
     gene            878..3553
                     /locus_tag="IVZ67_002447"
     CDS             878..3553
                     /locus_tag="IVZ67_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8166482.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3735..3974
                     /locus_tag="IVZ67_002448"
     CDS             3735..3974
                     /locus_tag="IVZ67_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166483.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3990..5363
                     /locus_tag="IVZ67_002449"
     CDS             3990..5363
                     /locus_tag="IVZ67_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166484.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5367..7001
                     /locus_tag="IVZ67_002450"
     CDS             5367..7001
                     /locus_tag="IVZ67_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166485.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
BASE COUNT         2509 a         1118 c         1404 g         2057 t
ORIGIN      
        1 atttatgtat ttttgtctta ttattacatg gaagagttat taacttagtt aatcgaaaaa
       61 gtacgactaa atttgcaaac tcattctggt ttttaaaaaa tattaataaa acagtggaaa
      121 agaatggagt ttataaaggt gattttcatt ttcaaaatgc tgagactatt gaacgagaac
      181 atcattattt cgatttatta gtgaatacag tggtagaatt agcgaaatgg gcaaagtcaa
      241 acgaaactta tagtcaagta cttaaaagaa ttatgagtca agaaacttca tttatctatg
      301 agttaagcaa gtcaaatttg aaatatactt ataatgaaga tgaacttgaa attttagata
      361 atccagaaaa ttcacaatgg attaaagagt tgaagagaga agactttatt gagacactaa
      421 gaattctaaa atcgttattt gatttgaaat tagccaaagc taccatcatg ggaattgaaa
      481 gagattcata ttattgggag gaaattggac tatatgctcc ccagattgaa ggtgaagata
      541 aagagaaaag gatagacaac attattaact atattaatag tttaagtgaa cctttaaaat
      601 gtagaggact tgattttata ggaacattag gatggacaga aacatactat gagaaaatat
      661 ataaagaaat aattgaacaa tattttccta aataatttaa taaggattga tttatcgtaa
      721 aagaaaaaag ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg
      781 gatatatttt tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca
      841 tacaaataaa aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat
      901 tcagaagcat ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt
      961 ttctacatta aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat
     1021 tattaactat ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc
     1081 tattaaacaa gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa
     1141 aattcggttt gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag
     1201 cgaaaagaaa aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag
     1261 aatagaatca tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa
     1321 tgaacaacca aataatcacg agacgattct tactattcag aattttcttc aattacttca
     1381 ccgaagattt ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag
     1441 attatgcaaa ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca
     1501 tcgtttatct agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat
     1561 tcttgaacag atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc
     1621 atcttttaga gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta
     1681 tcctgaatta acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt
     1741 tgacgaatgt ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac
     1801 ggctgataat aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca
     1861 agactttgta aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga
     1921 atccaatcaa gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt
     1981 gaaagaaggt caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa
     2041 tatcgaacaa aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga
     2101 aatagaaata gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg
     2161 cttaatcatt aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga
     2221 gaatgctcca atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat
     2281 gatttttcag ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc
     2341 atgctaccaa ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta
     2401 tgttttagga acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg
     2461 ctatttaaac gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa
     2521 gagcacaacg ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac
     2581 tacggttctt agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa
     2641 tcagggaggt gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag
     2701 ctcgttgatc tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca
     2761 gcttatgcca caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga
     2821 aaattatttc aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat
     2881 caagtttaca aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt
     2941 tggttggggt ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga
     3001 agcaactttt gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa
     3061 gcaacgcatt ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat
     3121 actgggctat cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca
     3181 gttaattgta gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt
     3241 gttggtgatg aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac
     3301 gatttgggga aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat
     3361 cccgtttgag gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag
     3421 gcaattgctt gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca
     3481 catgaatcgt caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta
     3541 ttttcgattg taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat
     3601 atttcttatt gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca
     3661 aacggaagta aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag
     3721 aaaggaaaat tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata
     3781 ttcaagaaca tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa
     3841 taagaggggt atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat
     3901 atttagaaaa tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt
     3961 tagttcattc atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg
     4021 tttatgaaga agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt
     4081 tagagaaata tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag
     4141 cggagcgtcc agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg
     4201 tgatctatgt ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta
     4261 ttgagttgtg ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt
     4321 ctttctcaca cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac
     4381 aacaacgaaa cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac
     4441 ggcaatattt gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac
     4501 aagaagtaca cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat
     4561 atggctacaa aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc
     4621 cttttcagcc ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca
     4681 aaggaggatc tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct
     4741 ttttacaagc ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt
     4801 acttattacg tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc
     4861 aatggaatac aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc
     4921 aaggaatttt tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt
     4981 tttttcaata cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg
     5041 aaaagcgaaa agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga
     5101 ttaaggcatt tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc
     5161 ttcaaaagcc gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga
     5221 aagaatggct tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc
     5281 ggattgaggt aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa
     5341 atattttaga aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt
     5401 atattcgcgt gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac
     5461 aaaatggacg acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg
     5521 aaggaatcag tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg
     5581 ctcggaacca tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata
     5641 tgttagatgt cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga
     5701 tctcggaaca atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga
     5761 gtttcggtga atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct
     5821 tagtgaaaga taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg
     5881 acacgaatgg tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga
     5941 tctatgcaaa atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc
     6001 aaggatatca aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac
     6061 gcaataaaat ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac
     6121 gacggaaggg gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt
     6181 cggaagaaga ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat
     6241 ggaaccatac aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac
     6301 cgatggcagc gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat
     6361 attattcttg ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc
     6421 gtgccgaaca agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag
     6481 aagttcttcc ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat
     6541 tggaacaaga actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa
     6601 aatgggaagc tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag
     6661 acgaattgac tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct
     6721 ttcaacaagg agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc
     6781 gttttcttgc ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga
     6841 aaataacctt tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg
     6901 ccgtggtaca acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg
     6961 ctatttttgt gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa
     7021 cgttagaggg gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga
     7081 ggtcctta
//
LOCUS       DACCZY010000129         6801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCZY010000129 DACCZY010000000
VERSION     DACCZY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.134"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..461)
                     /gene="rlmH"
                     /locus_tag="IVZ67_002451"
     CDS             complement(<1..461)
                     /gene="rlmH"
                     /locus_tag="IVZ67_002451"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8166486.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAG"
     gene            889..2178
                     /locus_tag="IVZ67_002452"
     CDS             889..2178
                     /locus_tag="IVZ67_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8166487.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2217..2822)
                     /locus_tag="IVZ67_002453"
     CDS             complement(2217..2822)
                     /locus_tag="IVZ67_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8166488.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3027..3491)
                     /locus_tag="IVZ67_002454"
     CDS             complement(3027..3491)
                     /locus_tag="IVZ67_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8166489.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3513..3833)
                     /locus_tag="IVZ67_002455"
     CDS             complement(3513..3833)
                     /locus_tag="IVZ67_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8166490.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(3977..5056)
                     /gene="pepA"
                     /locus_tag="IVZ67_002456"
     CDS             complement(3977..5056)
                     /gene="pepA"
                     /locus_tag="IVZ67_002456"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8166491.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5256..5606
                     /locus_tag="IVZ67_002457"
     CDS             5256..5606
                     /locus_tag="IVZ67_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8166492.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5829..6776
                     /locus_tag="IVZ67_002458"
     CDS             5829..6776
                     /locus_tag="IVZ67_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8166493.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
BASE COUNT         2025 a         1323 c         1278 g         2175 t
ORIGIN      
        1 cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg catcaattga
       61 tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat gacggcttcg
      121 ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg gatacctaat
      181 tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat tgctaaggca
      241 tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt ttcttttact
      301 tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac ttcgatcaat
      361 tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg gatcaggtat
      421 ttttctttta attttcctac tgagataatt ttgatattca tgttttctct cctttggtct
      481 tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt ttggctattt
      541 ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc cacagaaaac
      601 agtgttattt taccttattt catcacattc attaggcaaa catacgtttt ataagaattt
      661 atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa tcctatccgc
      721 gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg aaaacaatga
      781 tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa gtcaacaaat
      841 aagactatac taacagtaga taaatgaatg taattggagg aggaacacat ggatagaaaa
      901 aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa gttaggtctg
      961 gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta tgcagcgatg
     1021 ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc cggtgaaaca
     1081 gttgtggaaa atgtcaaagt caatgtagat tctgatatca ccaaagcggt agataaagtt
     1141 caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag cggttttggc
     1201 ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt ggaagcttat
     1261 agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt agtcacgaat
     1321 aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg tacgaaggta
     1381 aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa gatcaattca
     1441 gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt tggtgagcct
     1501 gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac ttcaggaatc
     1561 atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac agtcaatatc
     1621 aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc tttagttaat
     1681 atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc tgaatctact
     1741 tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt agtgaatatc
     1801 atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat cacaatggtc
     1861 gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc ttcatctgta
     1921 actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa agctggattg
     1981 aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg tgtagaactt
     2041 caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta ctatgaaggc
     2101 tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt aaaacaaaat
     2161 caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca acctctttat
     2221 tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc tttttttgct
     2281 ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct atctacccct
     2341 cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg ttttgcaacg
     2401 acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa tactgtacca
     2461 ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg gacgcatgtt
     2521 tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc ttcgaatcct
     2581 gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct tacttggccg
     2641 ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac aacttgacct
     2701 tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc tgttccttta
     2761 tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata agcaaaaatc
     2821 atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt gcattcactg
     2881 catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac catttattcg
     2941 taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag ttaattgatt
     3001 aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc gtcagaaacg
     3061 atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc tgtcataaag
     3121 cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc atattttttt
     3181 ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc ttctatattt
     3241 gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt ggctgtttca
     3301 gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt ataaaattgc
     3361 tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac ttctaccgct
     3421 tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat attcttatat
     3481 tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact ttctaggaac
     3541 gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa ttcttttccg
     3601 tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc tacatctatg
     3661 tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc aatttcaggc
     3721 atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa aaatacactc
     3781 tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat cattctttgt
     3841 cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt cgaactaaca
     3901 aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg ccagatttta
     3961 tagtttcact atcgcattat tttccgtata cgatcgtatt tactgttgat ttatccaaac
     4021 ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata ctgaacatcg
     4081 tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg attccttcgt
     4141 tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat tgataaggga
     4201 tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc atgatcaaac
     4261 ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg ccatttttcg
     4321 tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc ttgaatttat
     4381 tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg cctgcaatca
     4441 atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc attgtacagc
     4501 catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt ttgatcgttt
     4561 cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc tcttctcttg
     4621 attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt ccgcttgttc
     4681 ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta ccagtagagg
     4741 ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct agaggtacga
     4801 ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca tccatatgtg
     4861 ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca aaaatgccgc
     4921 ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc atataagcgc
     4981 gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg attcgttgaa
     5041 atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc tcagacttat
     5101 attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat tatagcttat
     5161 tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa aataagcaca
     5221 aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc gaactaagct
     5281 attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg gtagcttcaa
     5341 cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa aatatcaagt
     5401 catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa aaggaaccta
     5461 ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga atagaagatg
     5521 gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta ttagaaattt
     5581 caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga atacgcaccc
     5641 actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt tagatagagc
     5701 tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc gggaaaaact
     5761 agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat agttaaggag
     5821 agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac aatgaggaag
     5881 caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca gatatttatg
     5941 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt ggagcaatcg
     6001 taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc tttgatatcg
     6061 atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa gctgtacatg
     6121 gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat cgcctatcaa
     6181 atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat aaccttgtta
     6241 gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc ggctatcgag
     6301 gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt caaatcgaaa
     6361 cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc ccaatcgatt
     6421 atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac ggattcaaag
     6481 tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc tttgggattt
     6541 ggaccatctt cttcttttta tttggattgg tcacggggat tcctgtgatt cgcgaatata
     6601 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
     6661 tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg aacgctaaga
     6721 aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga aagtaaaaaa
     6781 aggatcaaga ctgcaaaaat a
//
LOCUS       DACCZY010000130         6798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCZY010000130 DACCZY010000000
VERSION     DACCZY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.135"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(67..249)
                     /locus_tag="IVZ67_002459"
                     /pseudo
     CDS             complement(67..249)
                     /locus_tag="IVZ67_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            236..433
                     /locus_tag="IVZ67_002460"
     CDS             236..433
                     /locus_tag="IVZ67_002460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166494.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(476..1051)
                     /locus_tag="IVZ67_002461"
     CDS             complement(476..1051)
                     /locus_tag="IVZ67_002461"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8166495.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1206..1732)
                     /locus_tag="IVZ67_002462"
                     /pseudo
     CDS             complement(1206..1732)
                     /locus_tag="IVZ67_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2189..3700
                     /gene="gadC"
                     /locus_tag="IVZ67_002463"
     CDS             2189..3700
                     /gene="gadC"
                     /locus_tag="IVZ67_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8166496.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3713..5113
                     /locus_tag="IVZ67_002464"
     CDS             3713..5113
                     /locus_tag="IVZ67_002464"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8166497.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5278..6713)
                     /locus_tag="IVZ67_002465"
                     /pseudo
     CDS             complement(5278..6713)
                     /locus_tag="IVZ67_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2102 a         1171 c         1226 g         2299 t
ORIGIN      
        1 taccactcca aacagggagc gtcaataatt ttgtgtaaat aactcgtcct tctgcaaaat
       61 aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga agcctttatg
      121 gcttctattt aagaattttt gattgtatgt atcaaagatg gatactagga aacgctctag
      181 tgattcttca ttttgaaact gctcttttct gcggctgtat ttcttgattt gtttattgaa
      241 agattcgatt gtagaaccag aaggaatgga gaacactttt gaactttaca acaaaactac
      301 tgttgataaa ttaattccaa ttttagttcc aactgattta aatggtcatg atgttttact
      361 tcgttttaac ggatttttag gtgaggatta tagataccta aaaatttctg atcctcaacc
      421 tttaacagaa taataaaaaa cgtgtaatca attatttgat tacacgtttt ttatttcaat
      481 cttcgttgta gtagtggttg cgacataccg aaaggtaatc ttcattacct cctactacaa
      541 tctgttcacc tgaataaact ggaataccat caacaatacg agcattcatt acagcctttt
      601 tattgcaaaa atggcaaata gttttaattt cctcaatttt atcagcaaat gtcaataatg
      661 cttcggatcc cttgaagaga ttattttgaa aatcattctt caatccatag caaattactg
      721 gtacttcctc attcattacg ataagtgata gatcaataat attctctcta cttaaaaatt
      781 gagactcatc gaccaaaata caatcaactt ttccgtactg atcaataatc tccactaatt
      841 ctccgtacac atccatttca gaagtaattg caatagctgg tttcgaaaca cctatacgac
      901 tctcaacttt tccaaatcct gatcgagtat ctattgcgct tgtcattatg ataactctct
      961 tcccttgttc ttcataatta tgagccactt tcagtagctc aatactcttt ccactattca
     1021 ttgttccata tctgtaaaat aattgtgcca ttaaatttat tcccttcttt tacaattcaa
     1081 ttattcctta attccatctg cttttatgca gctgctcgtt tattccttga tatgtctata
     1141 acatctacta tccctaaaac caatttataa gccattagag atggtattcc tgaacatctc
     1201 tcatattgtt ttttgagtgg tatatcatct gaaaaactga aggaatcatg aaaatagcga
     1261 catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc agcactttag
     1321 tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca atcaagtctg
     1381 cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc tctaatgtgt
     1441 cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccattg cggtcgcgcg
     1501 gaatctggag atgaagttcc ccatactcgg tcttgaccgt acggtcatag gagccgttac
     1561 gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta
     1621 actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat aatccattca
     1681 aatcgccttt gttcgctaga gtctttagga tttctgtagt aaaatcgttc atatggaagt
     1741 cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct
     1801 atttacacaa aatattttac actctcaaca aaagtatctt cgattaatac ttgctcaagt
     1861 tatgtgcagc tacgaaaaag taggtaccga ggtactttct ttttcgcagc tttttttgga
     1921 cttatgagaa gtacattcgc tggaagaata cgatataaaa cgagcttttt cactaaagat
     1981 attaatagag tgaaagaaac aagcttttgt tgtcagtttt ttcacttttc taaaaatgaa
     2041 aataaaaaat tctggaagtg gagaagaatt ttgcttgcta tactttgtct tgtagaaaag
     2101 aggtagacaa aataatgcaa gctcaaataa gcgattttcc ataaccttgg taggaatgac
     2161 aaattaaata ggaaagggaa gtaataatat gaataaaaaa aagttatctc tattcggttt
     2221 ttttgccctg acagcttcca tggttttgac tgtttatgag tatccgacct ttgcaacatc
     2281 acaattacat ttggttttct ttcttctttt gggaggtatt ttatggtttc taccagttgc
     2341 tttatgtgct gcagaaatgg caacagttga aggatggaaa gatggtggga ttttcagttg
     2401 ggttagtcaa actttaggag aacgttttgg ttttgcagct atttttttcc aatggtttca
     2461 aattacagta ggttttatta caatgatcta cttcatacta ggagcgctgt cgtatgtaat
     2521 taattttcca gagcttaata cagatccatt tatcaagttt attggtgtat tagtcatttt
     2581 ctgggtactg actttttcac agttgggcgg gaccaatcgt accgctaaaa ttgctaaagc
     2641 aggatttgtc attggaattg ttattccttc gatcattctc tttggtttag cagcagccta
     2701 ctttatcggc ggtaatccga ttcaaatggc tattaatgcg aaagctttta ttcctgactt
     2761 ttctcagctt tcaactttag tagtatttgt atcttttata ttggcttata tgggcgtaga
     2821 agcttcagca tcacatatta atgagctgga aaatccccaa agaaactacc cattggcaat
     2881 gtttttacta gtgattttgg ccatcgcctt agatgcaatc ggcggtttct cagtagcagc
     2941 ggttattcca caacatgact tgtctctcag tgctggtgta atccaaacat ttgaaacgct
     3001 tattttacat tttaacagtc atttacattg gttggtaaaa gtgattgctc tcatgattgc
     3061 ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt ccatcacgag ggatgtttgc
     3121 agctgctcag cgaggtctgt taccagtttt cttccggaaa actaataaac aagacgttcc
     3181 agttccgttg attatgatac aaggaattat cgttactatt tgggcagcag tcttaacttt
     3241 tggtggagga ggcaacaatt tatcattctt agtagcaatc tccttaactg ttgtcattta
     3301 tctcgtagga tatttactat ttttcctagg ttactttgta ttagttttta agaaaaaaga
     3361 cttaaaacga acttacaatg taccaggaaa aactgttggc aaaacgatta tcggtggaat
     3421 tggttttatt ttatcagtgt tcgcattgct tatctctttt gtaccaccag cttcaattgc
     3481 agctagtgag caccgaacat accaaatcat actacttatt agctttgtag tgactgttct
     3541 tttacctttt atcatttatg aagtacatga taaaagtgct cacacaacta ttgaggaacc
     3601 tactcacgtg aaagctgaag acgttaatcc cgctgtttat ccagctgcta gaggagagca
     3661 ccacgttaca aaaaatgatg aacatatttt aaaacattag gaggaattca ttatgttata
     3721 cggaaaagat aatcaagaag aaaaaaacta tttggaacca atttttggct ctgcaagtga
     3781 ggatgttgac ttgcctaaat ataagttaaa caaagaatcc attgaaccac gaattgctta
     3841 tcaattagta caagacgaga tgttggatga aggaaatgcg cgattaaact tagctacttt
     3901 ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg acccaaacgt tagaaaaaaa
     3961 tgcaattgat aaatcagaat acccacgaac aacggaaatt gaaaaccgct gtgtaaatat
     4021 gattgctgat ttatggcatg ctccaaataa tgaaaaattc atgggaactt caacgatcgg
     4081 ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa tttgcttggc gtaaacgtgc
     4141 tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac ctggtgatct cttctggtta
     4201 ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg gaactgagag aagtcccaat
     4261 ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg gattatgtgg atgagtacac
     4321 aattggtatt gtaggtatta tgggtattac ttacactggt cgttatgatg atatcaaggg
     4381 tctgaatgat ttagttgaag ctcacaataa acaaactgac tataaagtat acattcatgt
     4441 tgacgctgca tcgggtggct tttatgcacc atttactgaa cctgatctag tttgggattt
     4501 tcaattgaaa aatgttatct caattaattc ttcaggtcac aaatatggtt tggtatatcc
     4561 aggtgtgggt tgggtcttat ggcgtgacca acaatactta ccagaagaat tagtatttaa
     4621 agttagttac ttaggtggag aaatgccaac tatggctatc aacttctctc atagtgcagc
     4681 acaactaatt gggcaatact acaactttgt acgctatggc tttgatggtt atcgtgatat
     4741 tcaccaaaga actcatgatg ttgctgttta tttagccaaa gagatcgaaa aaactggtat
     4801 ttttgaaatc attaatgatg gatcagaatt gccagttgtg tgctataagc tgaaagaaga
     4861 tcccaatcgc gaatggacac tatatgattt atctgatcgt ctgttaatga agggatggca
     4921 agtcccagcc tacccattac ctaaagactt ggatcaatta attattcaac gcttagttgt
     4981 tcgagcagac tttggaatga acatggctgg tgattatgta caagatatga accaagcaat
     5041 tgaagagttg aataaagctc atattgttta tcataaaaaa caggatgtga aaacgtacgg
     5101 ctttactcac taaaattctt cagagaaata taaaagaagt taattaacta ataaaaggag
     5161 ttgtgattac cacacagctc cttaaatcta tttgaataaa tatatgatat tcatttgtta
     5221 cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt agttcagtca
     5281 aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa tcggatatat
     5341 aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa gtataatccg
     5401 ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa tgatattact
     5461 aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata aatacctaaa
     5521 gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct catttaaaag
     5581 acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa ataaattgtt
     5641 ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc gttgctcttc
     5701 atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa gtgagcggat
     5761 aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat tcagagataa
     5821 gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt tttgataaac
     5881 aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc tagagttgaa
     5941 tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag aaagttgctc
     6001 taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt tttcttcctg
     6061 actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac ctaataaaat
     6121 aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat gtttttctac ataatcaatc
     6181 aattgttgac cgctttgaat aattgattta tttagctgga tcaactctgt ccgatttgtc
     6241 tcatagatta tcgttagtaa caaacttcga taatcctttt caggaatttc aaaatggcca
     6301 tcagcattta agtactgcat ttgtttaaaa aaattaatca actcatttcg taagttgtaa
     6361 actttgctta acgaaatgaa ggtagtatca gctatattac tccaattgtg ttcccctgat
     6421 aaataatgtc ctagtacatt gaaaaagtca gagcgctgat agatgagttg agtgagtgcc
     6481 aacctcgaaa acgaagagtc gaatgcgact gtaaagctac ttctagattt ctccactgaa
     6541 ataatttgct gatggagtat gtcggtaatg cattccaagt cgttagtgac agtgagtggg
     6601 caaacgttca attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc
     6661 tcacaaatgt aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc
     6721 ctcctagtca ttttatacat aactattata actctaccaa tcggaatagt cctgtaatat
     6781 attgaatcaa aaatataa
//
LOCUS       DACCZY010000131         6788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCZY010000131 DACCZY010000000
VERSION     DACCZY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.136"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            21..911
                     /locus_tag="IVZ67_002466"
     CDS             21..911
                     /locus_tag="IVZ67_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8166498.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(984..1808)
                     /gene="nadE"
                     /locus_tag="IVZ67_002467"
     CDS             complement(984..1808)
                     /gene="nadE"
                     /locus_tag="IVZ67_002467"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8166499.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1820..3292)
                     /locus_tag="IVZ67_002468"
     CDS             complement(1820..3292)
                     /locus_tag="IVZ67_002468"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8166500.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3929..4291)
                     /locus_tag="IVZ67_002469"
     CDS             complement(3929..4291)
                     /locus_tag="IVZ67_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8166501.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4514..5266
                     /locus_tag="IVZ67_002470"
     CDS             4514..5266
                     /locus_tag="IVZ67_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8166502.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5406..6329)
                     /locus_tag="IVZ67_002471"
     CDS             complement(5406..6329)
                     /locus_tag="IVZ67_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8166503.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6344..6616)
                     /locus_tag="IVZ67_002472"
     CDS             complement(6344..6616)
                     /locus_tag="IVZ67_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166504.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2071 a         1352 c         1161 g         2204 t
ORIGIN      
        1 ccttttaaga ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg
       61 aagcattagc tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga
      121 atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga
      181 aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga
      241 gccttcgtgt cgataaaatc gatatcagtt ttccagaaaa aatattcaat gcttttgata
      301 cgaaagaaac atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg
      361 gtttagctac gatccatgat tttatcggga aagtggatga accacgctat tttatggatc
      421 ctcgaaggat ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac
      481 ctaatttttt aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt
      541 ctgaatttcc tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg
      601 tggagctagg aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc
      661 ctgattggta tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca
      721 aacacttgac gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta
      781 aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg
      841 gctgtacttt atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt
      901 actacagctg atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag
      961 ggacggctgt ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa
     1021 tgtcgtttat gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga
     1081 acttcttttc cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca
     1141 tctgcaatca ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt
     1201 tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat
     1261 ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca
     1321 gcatgatctg tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc
     1381 atacgttgac gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg
     1441 cttaatactt gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg
     1501 ggctggataa agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga
     1561 agacgaacag cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc
     1621 gttagttgcg ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca
     1681 aaagttttaa gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt
     1741 atttcttctt ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc
     1801 aggctcattt attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc
     1861 gttccatgat agccatttta tggttccagc attcagtaga cagatcgact gggtattttt
     1921 gcggattcaa gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt
     1981 ctttgatttc gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt
     2041 gtaaaactgg gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat
     2101 ggaacatgaa gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc
     2161 cttcagattt cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag
     2221 ttactttttc tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt
     2281 cgatcgatac tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt
     2341 ttgtcccaac accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg
     2401 cttcatccaa gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt
     2461 gttcccgaac acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga
     2521 agttgatttt gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca
     2581 acgtatcata agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa
     2641 atgcttcata atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg
     2701 gtatcccaaa tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg
     2761 ccgctctagt tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca
     2821 atagtggatc ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt
     2881 ggaaattgac catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt
     2941 ccacttggat taatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg
     3001 ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc
     3061 cacgcaagta agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc
     3121 gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt
     3181 caaaaacagc atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt
     3241 gatatagatc cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt
     3301 actcctcttg gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag
     3361 aaatcttcca ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa
     3421 ctccatctgc taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt
     3481 attttattca atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat
     3541 aaaataagta gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat
     3601 ataaccaaat gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct
     3661 ttcctttttt gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt
     3721 ttggaaaggc gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat
     3781 cacacgtttt taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa
     3841 tggcgagaca tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg
     3901 ttaccataaa gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta
     3961 tcactacaat atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt
     4021 tcactcctac atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat
     4081 agtcgatttc ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat
     4141 catatgtgtc taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa
     4201 tagccgtatt cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat
     4261 tactattcac aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt
     4321 agatccaatc ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga
     4381 acctttaaag acaaaaggat ttacctttta tcacttaata gtataacata atttcgtgag
     4441 aaaaaaatac aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc
     4501 aggaggaaaa ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc
     4561 gagaaccttg cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc
     4621 aggttatcgg atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg
     4681 aacaatggca ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca
     4741 ccagaaagct ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac
     4801 cctcttgaca actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga
     4861 caaagaaaaa tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg
     4921 acaagagatt cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat
     4981 gaatatggat aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga
     5041 tctcactctt tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc
     5101 tcataagaag tggttctctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact
     5161 aggaatgatg tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca
     5221 tggtgcagca aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc
     5281 caacaaaaaa gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt
     5341 tttatgctaa cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa
     5401 tatattcatc gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga
     5461 aacaattgaa atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca
     5521 agaagctcgc tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga
     5581 tagctttgca agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca
     5641 agctctttaa aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa
     5701 acaagtgatt catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag
     5761 ttataactta gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc
     5821 ttcaaataag taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc
     5881 tgttttccct tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa
     5941 tcatctagtg tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat
     6001 tcttcaagat accttcgctc tttttcttta tgatagcccg tcggtaaaag tgtcactttg
     6061 taatcataat caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga
     6121 ttggtataag gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca
     6181 tttgcattag ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc
     6241 tcatctacat atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg
     6301 agctttgcga tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt
     6361 cttgtaaata gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat
     6421 gcgaaaaact gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat
     6481 tcattggtat ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata
     6541 cggatccctt gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct
     6601 ctacataagt attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc
     6661 gtctcatagc aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc
     6721 aaagtctctt attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt
     6781 agtgaatg
//
LOCUS       DACCZY010000132         6619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCZY010000132 DACCZY010000000
VERSION     DACCZY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.137"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            258..2024
                     /locus_tag="IVZ67_002473"
     CDS             258..2024
                     /locus_tag="IVZ67_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8166505.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2126..3385
                     /locus_tag="IVZ67_002474"
     CDS             2126..3385
                     /locus_tag="IVZ67_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8166506.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3532..4833
                     /locus_tag="IVZ67_002475"
     CDS             3532..4833
                     /locus_tag="IVZ67_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8166507.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4836..5687
                     /locus_tag="IVZ67_002476"
     CDS             4836..5687
                     /locus_tag="IVZ67_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8166508.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5700..6515
                     /locus_tag="IVZ67_002477"
     CDS             5700..6515
                     /locus_tag="IVZ67_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8166509.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2117 a         1149 c         1339 g         2014 t
ORIGIN      
        1 tctacgttaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
       61 agataaattc aggacactaa atagtacagg aagtagatta aaacttttaa aagttttaag
      121 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
      181 catttttgaa agcgttgtct tttgtttctc tatcacttat agtgatgacg tcatattaaa
      241 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
      301 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
      361 aaaaagtata tgtgctttgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
      421 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
      481 tgacgagcga aacaaggcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
      541 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
      601 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
      661 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
      721 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gacttcttcg
      781 ggggagattt gcaaggtgtg agggatcatt tggattactt gacggacctt ggcgtcaatg
      841 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
      901 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
      961 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
     1021 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
     1081 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
     1141 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
     1201 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
     1261 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
     1321 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
     1381 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattc acagattcga
     1441 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
     1501 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
     1561 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
     1621 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
     1681 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
     1741 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
     1801 ttatttctga aggttctact cagtggctaa tagtatatga tcgtgaagat aagctctatt
     1861 tcacacggga actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
     1921 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
     1981 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
     2041 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
     2101 gttgaataca caggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
     2161 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
     2221 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
     2281 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
     2341 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
     2401 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
     2461 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
     2521 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
     2581 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
     2641 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
     2701 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
     2761 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
     2821 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
     2881 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
     2941 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
     3001 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
     3061 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
     3121 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
     3181 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
     3241 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
     3301 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
     3361 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
     3421 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
     3481 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
     3541 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
     3601 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
     3661 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
     3721 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
     3781 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
     3841 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
     3901 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
     3961 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
     4021 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
     4081 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
     4141 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
     4201 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
     4261 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
     4321 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
     4381 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
     4441 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
     4501 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
     4561 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
     4621 atgatctatc tgtttaacaa tggtggtccc ggaagcgtag gcggaggagc tggttctact
     4681 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
     4741 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
     4801 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
     4861 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
     4921 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
     4981 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
     5041 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
     5101 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
     5161 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
     5221 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
     5281 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
     5341 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
     5401 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
     5461 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
     5521 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
     5581 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
     5641 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
     5701 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
     5761 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
     5821 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
     5881 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
     5941 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
     6001 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
     6061 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
     6121 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
     6181 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
     6241 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
     6301 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
     6361 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
     6421 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
     6481 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
     6541 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
     6601 tcttagttga ttcttttct
//
LOCUS       DACCZY010000133         6387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCZY010000133 DACCZY010000000
VERSION     DACCZY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.138"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVZ67_002478"
     CDS             complement(99..1229)
                     /locus_tag="IVZ67_002478"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8166510.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVZ67_002479"
     CDS             complement(1878..3080)
                     /locus_tag="IVZ67_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166511.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVZ67_002480"
     CDS             complement(3140..3343)
                     /locus_tag="IVZ67_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8166512.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVZ67_002481"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVZ67_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVZ67_002482"
     CDS             4880..5692
                     /locus_tag="IVZ67_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166513.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1916 a         1306 c         1006 g         2159 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataata
//
LOCUS       DACCZY010000134         6280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCZY010000134 DACCZY010000000
VERSION     DACCZY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.139"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..333)
                     /locus_tag="IVZ67_002483"
     CDS             complement(<1..333)
                     /locus_tag="IVZ67_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166514.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVK"
     gene            complement(330..1022)
                     /locus_tag="IVZ67_002484"
     CDS             complement(330..1022)
                     /locus_tag="IVZ67_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166515.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(1028..1699)
                     /locus_tag="IVZ67_002485"
     CDS             complement(1028..1699)
                     /locus_tag="IVZ67_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8166516.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(1692..2033)
                     /locus_tag="IVZ67_002486"
     CDS             complement(1692..2033)
                     /locus_tag="IVZ67_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166517.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(2211..2909)
                     /locus_tag="IVZ67_002487"
     CDS             complement(2211..2909)
                     /locus_tag="IVZ67_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8166518.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2996..3466
                     /locus_tag="IVZ67_002488"
     CDS             2996..3466
                     /locus_tag="IVZ67_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166519.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(3509..3688)
                     /locus_tag="IVZ67_002489"
     CDS             complement(3509..3688)
                     /locus_tag="IVZ67_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166520.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3675..4013)
                     /locus_tag="IVZ67_002490"
     CDS             complement(3675..4013)
                     /locus_tag="IVZ67_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8166521.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(4029..4232)
                     /locus_tag="IVZ67_002491"
     CDS             complement(4029..4232)
                     /locus_tag="IVZ67_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166522.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(4247..5023)
                     /locus_tag="IVZ67_002492"
     CDS             complement(4247..5023)
                     /locus_tag="IVZ67_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8166523.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5322..5642
                     /locus_tag="IVZ67_002493"
     CDS             5322..5642
                     /locus_tag="IVZ67_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166524.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5660..6091
                     /locus_tag="IVZ67_002494"
     CDS             5660..6091
                     /locus_tag="IVZ67_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8166525.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         1829 a         1185 c          973 g         2293 t
ORIGIN      
        1 tttgactttt tcgtcaatag gggtattgcg tttttcgtca atagggtatt gattaattcg
       61 taggtaccta ttgattattt gattggtacc ctctttgtaa atgatttccc gattcaagta
      121 tccaaactta atcaaatcac ttacccatcg cgatatggtc tctttattca caccatataa
      181 atctgcaaag tactcattgc ctgcccaaca aaagcctctt tcattacaca aggccgttat
      241 ctctccgtat aacaacttag tatttggttt aagtcttttg tcgtacctta cgttggctgg
      301 tataatcgca taataacttc gatgttctgt catttttccc ctccaatatt taactttttg
      361 attgttttct ggtttaattt gatccctttg atttgatatt tatttttgaa attaatcaca
      421 cctattttgt gcttctctgt gtgatggatt ctgcagagtg ctgcaaatgt gtactctgca
      481 tgatcaactt ctttgcgctt tcgtcttcct agcgctttgt caaagtgatc gatgtcagct
      541 cctgttttgc cacagatgca gcagactctt tttgtgatgc atttgtagaa gtaatattct
      601 tgattcgcag gtaaaatctc atagccttct ttgaaaggaa tatgatgttc aaagatgaaa
      661 tctaagatga tatttgctaa gacattggca tcactcacag ttgtattcga ttcgtctttc
      721 aggcttattt tgcgccctgt gacaccttca aaacgaaagt agaagaattc cttccagaag
      781 tccgttggca tgcctgtatc gataaaaata tcgcctatca gcgcatagat gaagtttcgt
      841 tgctgtacgg tgaaacgtct aggatcaata aaacgaattt caatgactcg atcaccatcg
      901 tagccgtcat acatcgtctt tagtcgatca atgttcactt cctcattgat ggttgcgctt
      961 atgtcttttc ctttgaactt tttcagaacc gctgaatatg aatcgattaa tggtttaaac
     1021 actcatatca cttcttatct aattcttttc tcttagctgc tattgctcgc tccatcaagg
     1081 cacattgctc atagcttaac tgttcaatag tttcaacgtt atcagctaag agccctaatt
     1141 tatctgtctg ctcattaaca tattcgatta aggttttggt catatcttta cccatctgct
     1201 cattgaaagc ttctagaatc gtctctagca tgtttaattt ctttgtatcg attctaggtg
     1261 gtgttggaat atcttcccct tgaaatacat ataatcccag tccgtgtaga gccaatgctt
     1321 tcacaaagca tcgcttcaat gagttattga tttgcattgc atttggttta acaactggtt
     1381 ggtttcgata atctaaaaca ggaaataatt cggtttccgt gtgtccttta accgttactg
     1441 agacagatac ataagtccca gtttcatcca taagaaaagg tttatattcc tcaacaagaa
     1501 agtcttgatg agttccagaa acaaccctgt agtgtttgta ctcattaata gttaccgttg
     1561 cctgtggatc attctttttc ataatctccc acgcgtgagc ccaagataaa taatcaaaat
     1621 ttcctttttt cttgagaatt ttatttaact tacgactaaa aagtttttca aagttcgttg
     1681 tccctttgat ttcactcatc aaattctgcc tccatttcag caatgtattt cttacctggt
     1741 ccgtaataag agatatcaat caagttatct ctgtcgtact cttctagcgc atcaatcaag
     1801 ccatcttcga tgacgtaaat atattcaggt ttattcgaat gcttcgatag atggataaga
     1861 taaacatgat cccaaatagt tacatggttg cctaaatcat cttgatccca agctagttct
     1921 tcattcgtca aaagatttcg tctgattttt cgaccacttg tttcctcaat tttcggcttg
     1981 ccccaatcag gatcaatcaa atattgatct agagtggaaa gttctttttc catatgctaa
     2041 aatctcctta tgatgtgttt tctttgtgac tctttgcttg ccggcggagt cactttttta
     2101 tttgttgcca tgctttttgc ttgtcaatat gttgttggct taggatgatt ggtttatagt
     2161 atttccacca gcaattagca attgccgtcc ctattcttag cgcttcagct ctattcattg
     2221 tcatcaccaa aaagtctctg ttgtctgttc agttgatcaa tttccatacg gatcgcagtt
     2281 tctggcaacc acatttcaat aaatgaaaca gcatcatcga atctcttacg aggtaactcg
     2341 ccatatcttg ggattgaaaa ggtacgttta aattcagacc aaaattttga gaatactttt
     2401 ttgctgattt cttcataagc tcggctttct tttcccccta gaactcccat aactttcata
     2461 tttccttttt gcttaatttc aaactcttgt tgtccgctaa ttcgcatagt atctttaagc
     2521 atggaaacat cttttttaac atctttcatt tcttctaatt gatagatcat catatcttca
     2581 attgtttgag gaacagtatt cttgcgaata acatcttcca tttcgttgaa tgcttcaatg
     2641 tatttttgtt tgaagtagat agctttcttt cctgtaaacc ccatagccag caagaaaaat
     2701 ccatctctac taatgaagaa aactcgtcga tttctgccat atgaatctgg ttcattacct
     2761 tctacaaaca tctgtccaaa attggacaca tcttttttta gtgcatcaat atctcttaaa
     2821 acatgttgat gttttttctc gaagctttct gccacttgta agctcgtagt cacagcttct
     2881 ttatttttca aaattactaa ttcttgcatt atttcttctc tcctttttgg tataatttag
     2941 ttaaaaactg gtggtgttta ttttgaattt ttttcactta acaatttttc aatggatgtc
     3001 aataataagt tttatattga gtacgttttt aacagggata aaaataaaag gaatgcgccc
     3061 tcaactagaa gttgaactta atgcttctta cttcgctgca gatatgattt atacaaaagt
     3121 aatcatttct aactattcaa cagaaccagc tatgttagtt aatttagaac tttcatccgc
     3181 taacttaaac cgcagatggt cagctactcc atttaagaaa ttaattgcta aaggaggatc
     3241 agtcgatgat aatagaatat actctgaagc tgtaccgcta aatattcctc ctaaaagtgc
     3301 tatatcttgt taccttgcat ttgaagtagg gaaaattaat tttaataaac tccttaatca
     3361 ccaaacaaaa atgatattta ctctaaatag aacccaaatc cacaaaattg ttgatatcaa
     3421 aaatacaaac ttcccaatag agaaattagt gaaagaatta aactgagtaa tagtaatata
     3481 cattctagtt tctcttttct attcattttt agtcagcccc ctcggttggc tttttcgctc
     3541 tgtactcagc ttcagcttca tcaagcccca taaaaatcca aaccatgtaa acaatcgttc
     3601 ctatcaacgc ttgcctgctt ccccaaagtc ctaaagcgta gatgattagc ggtgcgctga
     3661 acactagcgc tctattaaat tttcccatcc gcttgcctcc ttaagatttc cgaaaaattt
     3721 gtttctaaaa attcaagtgt tttacttctt aaaaatagat atgtgtctct tccttcaact
     3781 ggataataga caaatccatt tttgtttttc tcgatatcga taatatttct atatcttggg
     3841 ttctttaaaa ctctagaagt aaaccaatca tattttctgt taatccgttc tagcacttct
     3901 ggcaacgtca tccatctacc agtatcatca gcttttttta actcctcata atccacttgg
     3961 gagataatta catagccttc tggaattggg atttttgctt ctagatattg cattagctgt
     4021 tcctcccttt acaattcgta catagtgata atcgaatcta taattctgtt tgcttctgca
     4081 gaagtctttt taccgtttaa aattaaagat aagtagcttt tgctaattcc aaatctttca
     4141 gcaagcatgg tgtaagttaa gaactttgaa ctttcgacat attctttgat tttttctcta
     4201 tctcgttgag tgatttctgc aatgtcagtc atactaaaac tcctttctaa ccaatttcct
     4261 ctaaatccat ttgagggtaa tatccttctt tttttagtaa ttcgtagata aatagacgcc
     4321 ctttctgtgt ccatttggta ttcattacaa ttttagtgcc accatcggct ttcgggatct
     4381 cagttgtatg agattttgtg tatccttggt tcatgtgttt tttgcacaat aaccattggt
     4441 taccgacttt tttctgaata cctagtttat gaagtaattt attcatctgt tgtggagaca
     4501 tcccataatc tgctgcaatc tgactaattg ttactgaatc tgtagaagat aatatgctat
     4561 ctaaatagga gattttaggt tcgtactctg caatcttttg ttctgcgatt aatcttccag
     4621 ttctttcttc ttttagttga gttgctaatt gaatgattgt atctggatta agcaaagctt
     4681 cttctacttt ttctggagtt agataacctc catgttttct aattgctggc aacacttcac
     4741 ttgttaccca acgtttaaat tttttggcag agggaagttt tgattttaag attaaactgt
     4801 acaaaccaga ttcgttgatg attgtcatct ctcttgattg acctgaggtc gtgattcgcg
     4861 accccatctt atcttccaaa tctacatgcc gcgacaaagc atctttagtg tttgaataac
     4921 ccaaaacact cgcaacgtct ttgcctacaa aatatggttc atcatttact aaaattgtcc
     4981 gaacttcgtt ttgttcgaaa ttaaaaattt gcggtgtgtt catatttctc attcctttct
     5041 tttatattcg taaacaaatt taacaacttt tttctaaatt caattgacag tatctgagta
     5101 atactctata ctaagagcat aattaaataa gacataaaaa cattgatttt aaagctttct
     5161 tggcggtctg catttacata tcaatagtgt tttttgttgt ctttttagtt gttaaatctg
     5221 tttacaagat atagtatagc tcattactca gaattagtca aattatttct gcgtattttt
     5281 ctaaactttt tttgtaaaca tttagaaagg ttgttttatc gatgaatacc tatgaaataa
     5341 taaaagactt ggcgaagagg aaaaagatat ctatacggca gttagaaatt aattttggat
     5401 attctaacgg ttatctagca aagtggaaaa ataatactcc taattcagat gaattaagtc
     5461 ggattgctga ctatttcgat gtctctgtag actaccttct aggtagagaa gaaagagaaa
     5521 cccctaaaca tgtggattta tcagaagacg atactgtttt ttcttttgat ggaaaagaaa
     5581 tatctaagga gacaatgcgt aaagcgattg caattgctaa agctttagag gaaaatgaat
     5641 agttggagtg atggtttgta tgtatttgaa attgaaagaa atgttgagcg aatacaattt
     5701 gaaagtagtt tatatggaga tgaaagagcc tggtttctac tatccaaaac ccagaatcat
     5761 ttttttgaat gaaaatctat acggtgaaac tgcagaagcc tttcatttat ctcatgaact
     5821 tgcacatttc agcgcctctc attttgaatt ttcagttttg tacgatactt ctacaacttt
     5881 tcactcaaaa tttgaaacag aagcagataa aatagctatt ctaattttat taaatatcta
     5941 tattgagaac gaactgactg atgaatctca gtttaatctc gaaaaattta tggagtatta
     6001 ttctattcag aataagctca gatacacttg ttatgctgtc tgccaatgct attttaagaa
     6061 aaaatattct tacgcaaggc aatatgtatg aatacatcaa gaattattaa aaaaaccgat
     6121 ttcagcaaaa cattaaaaaa gcccgtgctg caacacgggc tctttcctca tatatgagct
     6181 tctacaaaaa aaatcatatc acagaaatga ggaagaaaaa atggaaaaag agattttaat
     6241 aaaatctcca ggaaaaacct tgattaaagt aactgatgat
//
LOCUS       DACCZY010000135         6227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCZY010000135 DACCZY010000000
VERSION     DACCZY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.141"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(406..780)
                     /locus_tag="IVZ67_002495"
     CDS             complement(406..780)
                     /locus_tag="IVZ67_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8166526.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IVZ67_002496"
     CDS             complement(803..2203)
                     /locus_tag="IVZ67_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8166527.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IVZ67_002497"
     CDS             complement(2207..2617)
                     /locus_tag="IVZ67_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8166528.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IVZ67_002498"
     CDS             complement(2635..3126)
                     /locus_tag="IVZ67_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8166529.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IVZ67_002499"
     CDS             complement(3136..3927)
                     /locus_tag="IVZ67_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8166530.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IVZ67_002500"
     CDS             complement(3920..4693)
                     /locus_tag="IVZ67_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166531.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..5883)
                     /locus_tag="IVZ67_002501"
     CDS             complement(4849..5883)
                     /locus_tag="IVZ67_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8166532.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDS
                     DSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINL
                     FLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT         2007 a         1236 c          798 g         2186 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tttctgaact tagacagcaa
     6061 aaaatttctt gatgtttctt caattacatc aataaacatt ataaatagac tgatatccgc
     6121 caagaattca tctaataaac atagacctgt cgaatcaata attggtgata ttttattaac
     6181 aacatagttt cttaatgcta taaattcgtc actttctaaa taattat
//
LOCUS       DACCZY010000136         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCZY010000136 DACCZY010000000
VERSION     DACCZY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.142"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVZ67_002502"
     CDS             725..811
                     /locus_tag="IVZ67_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8166533.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVZ67_002503"
     CDS             complement(932..1621)
                     /locus_tag="IVZ67_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166534.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IVZ67_002504"
     CDS             complement(1866..2780)
                     /locus_tag="IVZ67_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8166535.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVZ67_002505"
     CDS             complement(2762..3142)
                     /locus_tag="IVZ67_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8166536.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVZ67_002506"
     CDS             complement(3592..3879)
                     /locus_tag="IVZ67_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8166537.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVZ67_002507"
     CDS             complement(3899..4123)
                     /locus_tag="IVZ67_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8166538.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVZ67_002508"
     CDS             complement(4763..5296)
                     /locus_tag="IVZ67_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8166539.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVZ67_002509"
     CDS             complement(5317..6159)
                     /locus_tag="IVZ67_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8166540.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCZY010000137         6093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCZY010000137 DACCZY010000000
VERSION     DACCZY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.144"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..124)
                     /locus_tag="IVZ67_002510"
     CDS             complement(<1..124)
                     /locus_tag="IVZ67_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HAP8166541.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKV"
     gene            complement(178..1995)
                     /locus_tag="IVZ67_002511"
     CDS             complement(178..1995)
                     /locus_tag="IVZ67_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8166542.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2009..2773)
                     /locus_tag="IVZ67_002512"
     CDS             complement(2009..2773)
                     /locus_tag="IVZ67_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8166543.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2801..3499)
                     /locus_tag="IVZ67_002513"
     CDS             complement(2801..3499)
                     /locus_tag="IVZ67_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8166544.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3511..4290)
                     /locus_tag="IVZ67_002514"
     CDS             complement(3511..4290)
                     /locus_tag="IVZ67_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8166545.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4306..5250)
                     /locus_tag="IVZ67_002515"
     CDS             complement(4306..5250)
                     /locus_tag="IVZ67_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8166546.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5296..6075)
                     /locus_tag="IVZ67_002516"
     CDS             complement(5296..6075)
                     /locus_tag="IVZ67_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166547.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1568 a         1284 c         1115 g         2126 t
ORIGIN      
        1 aaactttagg taatactatt ttttcgtcac acatatatag tgcgggtgct agttcagagc
       61 aatctgctcc aatgattttt ccttccattt cattattgtt taatgctgtt ttaaatgcat
      121 tcactagttc acaacgacgt ccacaactta atattaaaat attcaatttt taatctccta
      181 tctattcgaa gcatttgcaa aagtgacaat gtctttagcc aattgttcat cgtcttgcgg
      241 caggctcttg acaaaatcca tcactttttg tattgagtaa cctttgatgt ttcctacaaa
      301 gattttctca taaactgctt catcatttct ttccttatcc aacaatagtt cttcatatag
      361 tttttcccct ggacggatac ctgtttcaat gatttcgatt tcgtcttcgc tatacccgct
      421 caatcggatc atgtttttcg ctaggtctac aatcttcact ggttcactca tatctagaat
      481 aaaaatttcg ccaccttttg ctaatgcgcc agattgaata actaaacgac tagcttcagg
      541 aatcgtcata aagtatcgag tcatacgaaa gtctgttaca gtgattggac caccttgagc
      601 aatttgttct ctgaatactg gaatcacact accgcgagaa ccaaggacat tcccaaatct
      661 gacagctgag aatttcgtac agccttcttc attcaatcca gtcacaatca tttcagcgat
      721 tcgttttgtt gaacccatga catttggagg attgttagct ttatctgtcg ataccatgac
      781 gaagtttttc acttttgctg ctttagctgc ttctgctaca ttttttgtac cgaaaatatt
      841 gtttttgact gcttcttttg gattatattc catcagtggt acatgtttat gagcagctgc
      901 atggtagacg atatctggat gatattgttc catgatctca ttgattttct cgcgatcttg
      961 gatatctgcg atgatcggta cgatttctgt aggacaattt tgatgatgcg tgcgtaactc
     1021 acggtcaatc aagtagatcg agttctcccc atgtcctaaa agcaatagct ttgcaggatt
     1081 gaattgaatg atttgccgac agatttctga accgatcgat cctcctgcgc cggtgacaag
     1141 aatgactttg tctgtgatct gatctttgat ggattcaata tccagcttta cctcatctct
     1201 tcccagtaaa tccactacgt caattgtttt taattttgat acgctaattt tccctgaagc
     1261 aagttcttct atagaaggca tcgtgttgac tttcacacga gcggattcga ctaactcgaa
     1321 gattcgtcgt aatttttttc ttgataagga agggatagcg atcgttacca tttgaatgtc
     1381 atatttttca atcaattccg gaatatcttt taatgcacct aatacttttt ttcctgaaag
     1441 atacgtattt cttttatttg gatcatcatc tacaaacccg acaacgtgga tatcttgagc
     1501 cgttttggat ccaagaaagc tgttgtataa aatacgcccg ccttcgccag caccaacaat
     1561 caatgtattt ttagcagaat ctgctgatac gtatctcata ttttttgttt cgatgtagat
     1621 acgccagatc aatcggctac cgatgatcaa taagagcgaa agcagataag caaagataac
     1681 taaccgtaaa ctgtatcgcc gattaatgaa cagcagtatg aggatacttg aggcagcact
     1741 tgcagatagt gaactaaaaa ttgcaatgat ctctcttaaa tttgtatagc gattgatccg
     1801 agtgaaaact ttgaataaac taccataaaa taaataaaaa ccaatcgaca aaccgataga
     1861 gattaaaata agatccattg gtatagatac aaacggcttc atgaatttag ctgctgcaat
     1921 atttgcagca attaataaca agctgtccac aaaaaccaat gtggcaattt ttcttcttct
     1981 tgttaatata aacacttttt aattccccct aaaataaacc aaacttcttc ttttttacct
     2041 cttcatagtc tggataactg atttcatccc cgttgatcag atcctttgca acttgcttca
     2101 tctgtactac tttttcctta ccaaagtcct tggcaatctg ctcatagcac tcttttaagt
     2161 aaaaggtgcg cttagagact ccgtgtgcat cagaagcagt catctgcacg agattatgtt
     2221 tgaccattgt tttagctgtt ttctgaatat ctttgccaaa taccccaaca taacttggcg
     2281 cagtaagttg ggccaaacac cccatatcta ggaacggaag caaatgattc gggtcttcgc
     2341 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tctaacaact
     2401 caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     2461 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg atgagcgttc
     2521 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     2581 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     2641 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     2701 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     2761 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     2821 cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt gcttgtacca
     2881 tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt tcacgtacga
     2941 ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gctactactg
     3001 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg atctgatcca
     3061 ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     3121 acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat gaactaagtg
     3181 ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     3241 aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact gcaatgtttg
     3301 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     3361 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     3421 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     3481 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     3541 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     3601 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgc
     3661 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     3721 acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt tattatttgg
     3781 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     3841 cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagt
     3901 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     3961 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     4021 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     4081 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     4141 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     4201 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     4261 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
     4321 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc tgactttttg
     4381 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct tgatcgtacc
     4441 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     4501 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     4561 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     4621 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
     4681 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     4741 accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga catagtgatc
     4801 gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc cgccgaatgc
     4861 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
     4921 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
     4981 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca tcaatagaac
     5041 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     5101 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     5161 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     5221 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     5281 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     5341 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     5401 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     5461 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctagata
     5521 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     5581 agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag cttgctgcca
     5641 gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca gttttgattc
     5701 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     5761 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag aggatagctt
     5821 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat ttttttcttt
     5881 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     5941 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     6001 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     6061 gaatggcttt tccatagatt cactcctaag tat
//
LOCUS       DACCZY010000138         6073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCZY010000138 DACCZY010000000
VERSION     DACCZY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.145"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..606)
                     /locus_tag="IVZ67_002517"
     CDS             complement(76..606)
                     /locus_tag="IVZ67_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8166548.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(788..1444)
                     /locus_tag="IVZ67_002518"
     CDS             complement(788..1444)
                     /locus_tag="IVZ67_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8166549.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVZ67_002519"
     CDS             complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVZ67_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8166550.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVZ67_002520"
     CDS             complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVZ67_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8166551.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4171..4413)
                     /locus_tag="IVZ67_002521"
     CDS             complement(4171..4413)
                     /locus_tag="IVZ67_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8166552.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4445..5002)
                     /locus_tag="IVZ67_002522"
     CDS             complement(4445..5002)
                     /locus_tag="IVZ67_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8166553.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5015..5203)
                     /locus_tag="IVZ67_002523"
     CDS             complement(5015..5203)
                     /locus_tag="IVZ67_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8166554.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVZ67_002524"
     CDS             complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVZ67_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8166555.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1829 a         1276 c          892 g         2076 t
ORIGIN      
        1 aaaaatctta aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt
       61 tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg
      121 tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt
      181 tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa
      241 ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag
      301 ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca
      361 ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag
      421 ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt
      481 aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac
      541 tccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt
      601 tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag
      661 ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa
      721 atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa
      781 tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc
      841 aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa
      901 tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc
      961 tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag
     1021 tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa
     1081 agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac
     1141 gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa
     1201 ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac
     1261 catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag
     1321 atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat
     1381 aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat
     1441 tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct
     1501 tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa
     1561 tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa
     1621 gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc
     1681 catcttcaat atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt
     1741 ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg
     1801 catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa
     1861 caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca
     1921 taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc
     1981 atggtatatt accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta
     2041 tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca
     2101 tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc
     2161 atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta
     2221 cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg
     2281 acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt
     2341 cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat
     2401 attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa
     2461 acaccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     2521 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     2581 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaag
     2641 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     2701 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     2761 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     2821 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     2881 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     2941 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     3001 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     3061 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     3121 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     3181 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     3241 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     3301 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     3361 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     3421 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     3481 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     3541 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     3601 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     3661 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     3721 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     3781 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     3841 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     3901 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     3961 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
     4021 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa agaatacttc
     4081 atataaaata tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt
     4141 catttcaaca taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac
     4201 taatactaaa atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg
     4261 tccccaagtt cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt
     4321 tgcgatgatc cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat
     4381 accgccgacg attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta
     4441 cttcttattg tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag
     4501 cagattttgc tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg
     4561 ttactttgtc ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg
     4621 caacattcac attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga
     4681 tcaattcttt gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat
     4741 ctagatccac agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat
     4801 ctgtgttgac taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac
     4861 catctacatc tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca
     4921 attcaccatt catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg
     4981 tattcatgtt tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta
     5041 ttcctgactg tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca
     5101 acaatgtctt gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc
     5161 caaagataat agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt
     5221 ccactcgaga gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga
     5281 ctgccacttt gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt
     5341 gcatcaatac acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat
     5401 ggatatggat tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt
     5461 cttcgtgtaa atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc
     5521 catcttcccg tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca
     5581 agatgataat agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt
     5641 agtaattcgt taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg
     5701 cattgatcaa cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga
     5761 ttatttttaa ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata
     5821 actctaccga caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc
     5881 gctaccaaac attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca
     5941 attgcttact tattctctat ttacttttga ttgctggcta tacttaaagg atattattca
     6001 agattaaaaa ttgcaaagat tatgctcagt tattattaca agattctatt tatttattaa
     6061 attttttcgg aga
//
LOCUS       DACCZY010000139         6024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCZY010000139 DACCZY010000000
VERSION     DACCZY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.146"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(68..370)
                     /locus_tag="IVZ67_002525"
     CDS             complement(68..370)
                     /locus_tag="IVZ67_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166556.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="IVZ67_002526"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="IVZ67_002526"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="IVZ67_002527"
     CDS             complement(1127..1900)
                     /locus_tag="IVZ67_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8166557.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="IVZ67_002528"
     CDS             2044..2394
                     /locus_tag="IVZ67_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8166558.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="IVZ67_002529"
     CDS             2375..3091
                     /locus_tag="IVZ67_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166559.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="IVZ67_002530"
     CDS             3091..4539
                     /locus_tag="IVZ67_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8166560.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="IVZ67_002531"
     CDS             complement(4610..5119)
                     /locus_tag="IVZ67_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166561.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="IVZ67_002532"
     CDS             complement(5109..5834)
                     /locus_tag="IVZ67_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166562.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2051 a         1050 c         1170 g         1753 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aata
//
LOCUS       DACCZY010000140         5977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCZY010000140 DACCZY010000000
VERSION     DACCZY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.147"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            71..1021
                     /gene="yidC"
                     /locus_tag="IVZ67_002533"
     CDS             71..1021
                     /gene="yidC"
                     /locus_tag="IVZ67_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8166563.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1200..2174)
                     /gene="lacD"
                     /locus_tag="IVZ67_002534"
     CDS             complement(1200..2174)
                     /gene="lacD"
                     /locus_tag="IVZ67_002534"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8166564.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2171..2629)
                     /locus_tag="IVZ67_002535"
     CDS             complement(2171..2629)
                     /locus_tag="IVZ67_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8166565.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2626..4089)
                     /locus_tag="IVZ67_002536"
     CDS             complement(2626..4089)
                     /locus_tag="IVZ67_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8166566.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(4071..5021)
                     /gene="pfkB"
                     /locus_tag="IVZ67_002537"
     CDS             complement(4071..5021)
                     /gene="pfkB"
                     /locus_tag="IVZ67_002537"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8166567.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            5164..5913
                     /locus_tag="IVZ67_002538"
     CDS             5164..5913
                     /locus_tag="IVZ67_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8166568.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         1812 a         1303 c         1033 g         1829 t
ORIGIN      
        1 ttgattgaat tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag
       61 gaagtattct atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct
      121 gctcttttta tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcaggaat
      181 cgtttatcga gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt
      241 caattggtct tacggctggg cagttattgc aatgactgtg atcgttcgta tcatcatctt
      301 gccactaggt atcagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt
      361 aaaaccacaa gttgaagcag cacagcaaaa gctaaaagct gcaacaacac gtgaagaaca
      421 aatggcagca caagcagaaa tgcagcaagt atatcgcgaa aatggattga gtatgaccgg
      481 cggaatcggc tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtattttac
      541 ggcacgctac acagaaggta tccgggaatc atctttttac ggcatcgatc taggctcacc
      601 aagtatggtg cttgtggcca tcgcaggggt cgcttatctg cttcaaggat atatttcaac
      661 aatcggtatt cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc
      721 tatgatcgtc ttcatgtcaa tcagtgcgcc agccggtgtt acactttact gggtagtcgg
      781 tggtgtgttc agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa
      841 agcacaagtg gcagaagagt tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa
      901 agacgtgaca gaatcagcaa agccagctaa caacaaacaa gcaacaaaac aaatcaaaca
      961 accaaaaaac agtacaaaca acaaaggccg caacgcagga aagcagcaga agcggaagta
     1021 gatagtaaat aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac
     1081 caagattttt gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa
     1141 tcatcgattg atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct
     1201 tatctatcaa cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttctttaata
     1261 ttttgttttc cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa
     1321 ggtgccactc ccttcgccca tgttgctcgc ccacataaga caccattgaa tgtcgaacct
     1381 gattgttttg caaaacgtaa tgtatcttga aatagcgtag cattgactcc cgcacttaaa
     1441 aaaataaacg gttgttcagt cactttcgat tgttcataga aatacgcgga tgcctctgct
     1501 tgactataac aagtctcttg cccatatcct tccacaaatc tcatattcac tggcacttct
     1561 actttcaata catccacttg ataacgttct tttgaaaatt ctcgcatggc ttcaataact
     1621 ttatgtggtt ttacttttgc ataggcaaga gaagacggat cgccaatagt tgcatcataa
     1681 gaaagaatct ctaaaaagaa aggtatgcct tctgcttcgc attctgaacc aatccgttct
     1741 ataactattt gtttttgcac attaatctct gctggttcat caacgtcata gtacaataaa
     1801 aatttacaag catccgcacc agcttctttt aaccttttca ccgaccaaat attcaataaa
     1861 tcaggtaatc ttcccggtat tttcgtatca tagccggttt tttcataagc tagcagtaag
     1921 ccacattctt tctctcgaag cgctgctgca ggcaggccgt attctggatc aagtaaaata
     1981 gctgaggcat agggagtcag gtattgagaa accatctgtt taaattggat gatgtcttct
     2041 gatgtagctt ctttatattg gttgatcatt ttcttcaaag caccacgctg gtcgattgct
     2101 aatgcgctaa taattccagc ttcatccgtc aatcgcttca aacgttcctg tttttctata
     2161 ctcatcttct tcatagatct tcacctattt ctttagttaa tattgtggct atttctgttt
     2221 ttgattttgc ttttctcaac tgttctgtaa cctcttttcg catcaatgat cgtgcaacga
     2281 gagacaataa tttcaaatga gtcgttcctg cttctgaatc tggaataaat aacgcaaaaa
     2341 taaaatcaac tgactgtcta tctaaactgt tccattcaat tcctttgttc agtgtggcga
     2401 taatcatact aggttcgttg attgtttgag acttcgcatg agggatagca aaacctgaca
     2461 ttattcctgt agtcccttct ttttctcttt ctagaagttt ttgatatact tctttttcgt
     2521 caagagcgat ccctaaacag aatgtttgct ttgctaaaaa cttaaatgct tcttcttgac
     2581 tatccaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg ataagtctcc
     2641 tgctcttcct ttttcgataa ctgtacgatt tgctgcttta ttatccatct tcttgtcttt
     2701 atcagttact cttttctttt ggattaatcc tagtatcact ccaccaacga tcgaaccaat
     2761 aagaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaacacta aaaaaccacc
     2821 atgtggtgct ggaacttgga cgccaaacaa ataagttaag acagcagcaa ttgatgagcc
     2881 taacatcatc gtgggtaaca ctcggatcgg gtctttcgca gcaaacggaa tagctccttc
     2941 cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat cattggtatc
     3001 aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg caatacaagc
     3061 tgctgacaca ccagccataa aagtcgtgtt tccttgtgcc aaaagagctg ttcctgttac
     3121 atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatacagc caacaatcac
     3181 gccaagaaca agtggattcg aatgttcaaa tccagctaaa aagctcatca ttccttcatt
     3241 tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg ctacaccaat
     3301 aactggatat aagaagattg ccttcaagcc atctagttgt ttaggtaaaa ttgtcagtag
     3361 cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatac ctcctaaaaa
     3421 tccagtaccg ccattcatgg ctacgattcc accaacaaat ccaacgacta accctggacg
     3481 tttcgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc ccattgctgc
     3541 atcgccaact gcttttacca ttgccgctgt agcattgtat tgtgcatgat ctggatcaaa
     3601 agaatagatt ccccaaacgg caaatgaaat agcaatcaat actccgccag ctaccacgaa
     3661 aggcaacata tgagagacac cgttcattaa atttttatag atgctacgac caaaattagc
     3721 accactcatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta agacagtacc
     3781 ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg tactgacaga
     3841 aacatcaatg actcgttttc ccgcaaatct ttctgaatgg acatctttat ctgcggcaat
     3901 aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattttcga tcccaatttg
     3961 tccatgggtc tccactttga tcttgatgcc ttttttccta gctgcttgtt ctaatgcctc
     4021 ttgcgccatg taagtatgcg caattcctgt cggacaaccg gttgctgcaa ttagttgata
     4081 ttttcccatg tttcaaacct ctcttccttg attttgattt gtttacttaa ttcctccaca
     4141 tccaaaaaat cagtcagtcc tttacgaaaa gcggttgagc tgcctgctgc tatgctttta
     4201 cgtaacgttt catcaagcgg tcgtcgattc atatatcctg ctaaaaaagc gcctaacatg
     4261 gtatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc tcgaaacacc
     4321 ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac attttctgca
     4381 cctaactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac catttttcgt
     4441 ccgaaccaag aagaaagttc ttcctcgttc ggtttcagca aaaacggtcg atagggtaaa
     4501 caatccatgg cttcttgatc actactatca ataatcaaat gaatcccctt ttcctgacaa
     4561 atccgactga tttctattaa aacctttgga gacagccctc ttggtaaact cccagaaaca
     4621 caaagataat cttctgcttg taatgctctt atttgattta aaaaattatg gacagctgtc
     4681 tgcggtattt taggtccttg attcactaat ttgtattctt ctcccgtttg attgacttgt
     4741 gtaaatacat tgatacgcgt cataccaggc acttcaataa actctgtttg gatttgtttc
     4801 tgctgtaaat agtcttctat atactttcct gtaaacccag cagaaaaacc taatgccgta
     4861 cttgggaaat ctagcatttt taatactagt gacacattca ccccttttcc gtttgcctga
     4921 atgtcatcct cttttgtgcg gtttaccata aaaggatgca tctcctctgt ttcaataaat
     4981 agatcaattg ccaaattcat cgtgcatgtg tagatagaca taaccaacga cctccttgac
     5041 tctattatga aatacgcgaa agagaaaatg ttttcttaat cgtcccaaca caaagaaaac
     5101 ctttacattt ataaactcgt cgtgctattt tttaactaac ttttggtgaa agaaggaaag
     5161 aatatggatc acaaattaag tgaaacagaa cagtacctct ggaactttat cgaacaccac
     5221 atcctagaga tccctaacta ctccatcgtg aaactaagtg aacaagcaaa tgtctcaaca
     5281 gccaccattg tgcggacaat gaaaaagaaa ggctatgaag gatttacttc gtttaagcac
     5341 catctaaaag aaggagaaaa caagaacatc aatttttctt tcttagataa agtagacaaa
     5401 ggtatccgtc gagccattct aaaaaatgaa caagaagtgg tacgtacgat caatatgttg
     5461 gaaatcggaa atattgagga tgcaatacaa agaatcaaat ttgctgaccg ggtttttgtc
     5521 tttgcacgag gattttcaga aatgattggt caagagatgc tcgtaaaact tcaattaaca
     5581 gggaaaaatt gtcaaatgca tactgatcca gaaatcatca aaagcatcag tcggaaacta
     5641 actaagaaag acagcgttat ttttgtttct ttgaacggcg agacaaaaga actcgtcacc
     5701 gccgcaaaga attgctatga tgcagagatt ggttcgattc tagttacagc caatcattcc
     5761 tctacactga aagactattg cgagatcaat ctcgtaggtt tcaaatcaga aggttcttat
     5821 ttccctgatt atgaagtacg gtcacgtttg cctctccaga ttatagcgag aatcctgctt
     5881 gacgcctatg tattgcgtat gggggagaat tgaaaagcag tttattatgg ttggttacag
     5941 tttaaaacat cacatttttt attctttata ttgtatg
//
LOCUS       DACCZY010000141         5873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCZY010000141 DACCZY010000000
VERSION     DACCZY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.148"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..636)
                     /locus_tag="IVZ67_002539"
     CDS             complement(<1..636)
                     /locus_tag="IVZ67_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8166569.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNS"
     gene            784..2073
                     /locus_tag="IVZ67_002540"
     CDS             784..2073
                     /locus_tag="IVZ67_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8166570.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2199..3278)
                     /locus_tag="IVZ67_002541"
     CDS             complement(2199..3278)
                     /locus_tag="IVZ67_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8166571.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3387..3827)
                     /locus_tag="IVZ67_002542"
     CDS             complement(3387..3827)
                     /locus_tag="IVZ67_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8166572.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3884..4294)
                     /locus_tag="IVZ67_002543"
     CDS             complement(3884..4294)
                     /locus_tag="IVZ67_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8166573.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4475..4909)
                     /locus_tag="IVZ67_002544"
     CDS             complement(4475..4909)
                     /locus_tag="IVZ67_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8166574.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5009..5857)
                     /locus_tag="IVZ67_002545"
     CDS             complement(5009..5857)
                     /locus_tag="IVZ67_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8166575.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2148 a         1102 c          984 g         1639 t
ORIGIN      
        1 gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa aagccactac
       61 ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc
      121 taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga
      181 aaaggagata gacaaaccgt acaatgctaa aaatgcatta atctttttca ttttcgtata
      241 caaataagag gcactatagt ttgtttgctg acttaaactg ataatcgaat ttgtctgaca
      301 gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag
      361 acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg
      421 cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat
      481 gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat
      541 aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc tttttttctt
      601 caagagaaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa
      661 ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa aacagtaaac
      721 atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag gagtgagaga
      781 gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt atcgcttact
      841 ataaatcaga cagtccacac agaggagata gaaacagcca aatggacaga aaccaagtgg
      901 gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc aggagtcgca
      961 aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc aggagactta
     1021 gcacctatac tacaatcagc tgttacattt gagaatgttg atcccgcttt gtcgattggc
     1081 tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt caccgttaca
     1141 ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga tggactaccg
     1201 aacgatcatc atcatttaac gccgtatagt cagcggcaga caattgagca agattcagct
     1261 atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag
     1321 ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga
     1381 gaagaacccg atacagaaac tcaaccagag ccagacaatg aatcaaaacc tgaaataacc
     1441 cctggagaaa aaccagatgt agacccagaa gaaaaaccgg atgtgactcc agaaccagat
     1501 acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa
     1561 aatccagaaa aaccagaggt agccccagaa gaaaaaccag atgtaactcc agaaccagat
     1621 acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa
     1681 aatccagaaa aactagaggt agatccagaa gaaaaaccag atgtgactcc agaaccagat
     1741 acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat tcaagaggat
     1801 ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga agagcagctg
     1861 aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa aagtagcaag
     1921 gatacaacct ttgctagtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca
     1981 caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct
     2041 gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt
     2101 tttcaaataa aaagaaatat ccagaaaacg accccaaaat accagattga aatctgatat
     2161 ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt
     2221 ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga
     2281 atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat
     2341 gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac
     2401 tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct
     2461 tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc
     2521 agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc
     2581 aaggcgattg tagtgagcaa cgtgtttaaa agagcgataa caaattgagc ttctaatacc
     2641 aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa
     2701 aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa
     2761 aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa
     2821 tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata
     2881 taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatca
     2941 ataacggact ggatcatatc aaatgtttga tgaataagca tcgggatata ctttgtgaat
     3001 gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg
     3061 attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat
     3121 gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat
     3181 aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg
     3241 acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata
     3301 agaaagagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
     3361 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
     3421 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
     3481 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgaggagtc
     3541 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
     3601 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
     3661 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
     3721 taggtaagat tcagcggctt gaggagggaa gtgtagtgtc taatgatttc cttagaggcg
     3781 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
     3841 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
     3901 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
     3961 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
     4021 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
     4081 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
     4141 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
     4201 ctgcaatatt caattcgaat gtacgatcgg gattatgaac ttctccagag cgtccgcctg
     4261 tgttgatcat tttcgtagaa tacatttttt tcataaaatt accaccttta attatatttt
     4321 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
     4381 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
     4441 atatcagaga caaaaactaa tctcatcata aacattacat atatttctca aaaagttgtt
     4501 ttctttcttc aaaacttact tcacgatgga cctgatgaag catccataaa taaccaaaag
     4561 gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct
     4621 ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat
     4681 tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt
     4741 catccagcat atggaatctc gtgccataca aggtaaaaat gacttcatta ttgcctctag
     4801 ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg
     4861 ctttcaaact atcatttaat acaaaatcta tttccgttcc tttatgcata aagatctcct
     4921 ctccttttca gtcgaacagt tactagattc ttttattata ccagttaaag aaaacgcatt
     4981 caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc
     5041 ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca
     5101 atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact
     5161 ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta
     5221 agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat
     5281 tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat
     5341 aaagaaactc ttctattcca aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa
     5401 aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg
     5461 tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg
     5521 cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg
     5581 caatgattaa aggaaaaaac aagcttccga aaagtacttt aaaaaagggt tttttaccta
     5641 aaaatagaag agctagtacc aacataacga tattcaacac taaaataact atagctcggt
     5701 caaatcctaa aacagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat
     5761 gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct
     5821 taatgtatgt ccatataaat tgtctcgttt ctttcattta tccacttcct taa
//
LOCUS       DACCZY010000142         5866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCZY010000142 DACCZY010000000
VERSION     DACCZY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.149"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            145..1170
                     /locus_tag="IVZ67_002546"
     CDS             145..1170
                     /locus_tag="IVZ67_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8166576.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1361..2605
                     /locus_tag="IVZ67_002547"
     CDS             1361..2605
                     /locus_tag="IVZ67_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8166577.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2758..3705
                     /locus_tag="IVZ67_002548"
     CDS             2758..3705
                     /locus_tag="IVZ67_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8166578.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3839..4468
                     /gene="upp"
                     /locus_tag="IVZ67_002549"
     CDS             3839..4468
                     /gene="upp"
                     /locus_tag="IVZ67_002549"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8166579.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4607..4849)
                     /locus_tag="IVZ67_002550"
     CDS             complement(4607..4849)
                     /locus_tag="IVZ67_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166580.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4970..5042
                     /locus_tag="IVZ67_002551"
     tRNA            4970..5042
                     /locus_tag="IVZ67_002551"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5003..5005,aa:Thr,seq:cgt)
     gene            complement(5161..5799)
                     /locus_tag="IVZ67_002552"
     CDS             complement(5161..5799)
                     /locus_tag="IVZ67_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8166581.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         1983 a         1036 c         1226 g         1621 t
ORIGIN      
        1 ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca
       61 aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta
      121 tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa
      181 gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac
      241 ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg
      301 agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat
      361 gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta
      421 actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc
      481 acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt
      541 gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat
      601 catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga
      661 attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta
      721 cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt
      781 gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac
      841 tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa
      901 tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa
      961 gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct
     1021 aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt
     1081 tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa
     1141 gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta
     1201 tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc
     1261 gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag
     1321 gaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
     1381 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
     1441 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
     1501 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
     1561 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
     1621 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
     1681 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
     1741 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
     1801 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
     1861 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
     1921 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
     1981 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
     2041 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
     2101 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
     2161 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
     2221 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
     2281 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
     2341 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
     2401 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
     2461 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
     2521 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
     2581 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
     2641 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
     2701 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
     2761 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
     2821 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
     2881 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
     2941 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
     3001 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
     3061 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
     3121 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
     3181 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
     3241 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
     3301 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
     3361 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
     3421 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
     3481 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
     3541 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
     3601 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
     3661 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
     3721 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
     3781 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
     3841 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
     3901 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
     3961 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
     4021 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
     4081 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
     4141 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
     4201 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
     4261 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
     4321 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
     4381 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
     4441 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
     4501 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
     4561 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
     4621 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
     4681 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
     4741 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
     4801 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
     4861 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
     4921 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
     4981 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
     5041 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
     5101 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
     5161 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
     5221 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
     5281 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
     5341 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
     5401 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
     5461 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
     5521 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
     5581 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
     5641 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
     5701 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
     5761 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
     5821 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaa
//
LOCUS       DACCZY010000143         5852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCZY010000143 DACCZY010000000
VERSION     DACCZY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.150"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(124..582)
                     /locus_tag="IVZ67_002553"
     CDS             complement(124..582)
                     /locus_tag="IVZ67_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8166582.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(595..1920)
                     /locus_tag="IVZ67_002554"
     CDS             complement(595..1920)
                     /locus_tag="IVZ67_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8166583.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1937..3349)
                     /locus_tag="IVZ67_002555"
     CDS             complement(1937..3349)
                     /locus_tag="IVZ67_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8166584.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3366..4130)
                     /locus_tag="IVZ67_002556"
     CDS             complement(3366..4130)
                     /locus_tag="IVZ67_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8166585.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4234..5109)
                     /locus_tag="IVZ67_002557"
     CDS             complement(4234..5109)
                     /locus_tag="IVZ67_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8166586.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5364..5448)
                     /locus_tag="IVZ67_002558"
     tRNA            complement(5364..5448)
                     /locus_tag="IVZ67_002558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5412..5414),aa:Leu,seq:caa)
     gene            complement(5466..5537)
                     /locus_tag="IVZ67_002559"
     tRNA            complement(5466..5537)
                     /locus_tag="IVZ67_002559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5503..5505),aa:Gln,seq:ttg)
     gene            complement(5547..5619)
                     /locus_tag="IVZ67_002560"
     tRNA            complement(5547..5619)
                     /locus_tag="IVZ67_002560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5584..5586),aa:His,seq:gtg)
     gene            complement(5681..5765)
                     /locus_tag="IVZ67_002561"
     tRNA            complement(5681..5765)
                     /locus_tag="IVZ67_002561"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5729..5731),aa:Leu,seq:caa)
BASE COUNT         1784 a         1248 c          894 g         1926 t
ORIGIN      
        1 aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg aaacatatca
       61 tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt ttttcatcgt
      121 acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt ttaataaatt
      181 tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg cttgctccaa
      241 cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt tactttcaac
      301 ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat ggatcagtat
      361 ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata tattatcgat
      421 tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt atccaactcc
      481 tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa tttgaccact
      541 agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc acctctactt
      601 taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt tttttgcgac
      661 tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt gataagaacc
      721 ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca tcaatccttt
      781 atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg caccaacata
      841 tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt gattaggaac
      901 gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat cgacgatata
      961 ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc ggcgtatttt
     1021 atcagccatt tctttggtat ttttctctcg tccatccatg accatattag cacgtgtata
     1081 ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta agtaattatt
     1141 cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg ttttttgcca
     1201 agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta gtttttcaat
     1261 aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat accagccaaa
     1321 gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt aattcactgc
     1381 aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta cattcggata
     1441 tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca aaatccatgc
     1501 tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga ttgagcgcat
     1561 cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat gtttaagtgg
     1621 aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa
     1681 atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa tatttgaaaa
     1741 accttttctt ttcagcataa atttaacaat caagtacata tccatatttc tttcttcatc
     1801 aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca aaatggcttg
     1861 aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct gttttttcat
     1921 catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata acataagtca
     1981 cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc ccgcttatcc
     2041 ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct acccgtaggc
     2101 gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct tgtagcattt
     2161 ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct tctaaggtta
     2221 cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt ctcttattga
     2281 tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt ggaagaatca
     2341 aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat gctccgaatg
     2401 taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg gcaaaaagtg
     2461 catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca ggctcagtga
     2521 tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc atctctttat
     2581 tttctttata agctgcttta tagatagcta gacatatagc tggtatacca aacatcatta
     2641 ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata aagcgtgtca
     2701 tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg taataaatat
     2761 acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa aacataaata
     2821 tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca atcccaattt
     2881 ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca
     2941 tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat ttttgttctt
     3001 taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca aaaatcccca
     3061 tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc gctaaaaaac
     3121 cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca actcctatag
     3181 caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt actgggatgt
     3241 tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc gctgcaactg
     3301 gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt tttattcctc
     3361 ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt cactttttga
     3421 gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac aaaacttgaa
     3481 ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat cgttttatct
     3541 tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt gatttgtcgt
     3601 tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc gacatgacga
     3661 acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt tgctaatgta
     3721 acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc taaatgagtt
     3781 ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg atacagttga
     3841 tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc tccgctttcg
     3901 ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa atggactcct
     3961 acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc cggcatattt
     4021 gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat cccataattc
     4081 acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat ttcattctcc
     4141 ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac tatgtgtcat
     4201 ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta gatgaattgc
     4261 caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga tgatatcttt
     4321 aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa aaaaaccaat
     4381 attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt tgatatgaac
     4441 cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg atttttctag
     4501 atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac tattaggctc
     4561 tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg accctaactc
     4621 ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat attcatttgg
     4681 ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt ctctttttcg
     4741 ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat ctaatggaga
     4801 ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa attcgattgg
     4861 aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta atacaaataa
     4921 tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc caatcccttt
     4981 ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat gattcagctt
     5041 tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac aacttgcaga
     5101 ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg acctaagaaa
     5161 gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt tacaaaaata
     5221 gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg ataatacttg
     5281 tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa
     5341 gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg
     5401 gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa atttaaaaat
     5461 aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac
     5521 cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca cgaatgccgg
     5581 agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag atgttattta
     5641 atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc cggggtcgaa
     5701 ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa ttccgccacg
     5761 ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt
     5821 ttgcagacct ctgccttacc acttggctat ac
//
LOCUS       DACCZY010000144         5796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCZY010000144 DACCZY010000000
VERSION     DACCZY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.151"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            203..574
                     /locus_tag="IVZ67_002562"
     CDS             203..574
                     /locus_tag="IVZ67_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166587.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(858..1943)
                     /gene="xerS"
                     /locus_tag="IVZ67_002563"
     CDS             complement(858..1943)
                     /gene="xerS"
                     /locus_tag="IVZ67_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8166588.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2277..2510
                     /locus_tag="IVZ67_002564"
     CDS             2277..2510
                     /locus_tag="IVZ67_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8166589.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2507..2914
                     /locus_tag="IVZ67_002565"
     CDS             2507..2914
                     /locus_tag="IVZ67_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8166590.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4051..4569)
                     /locus_tag="IVZ67_002566"
     CDS             complement(4051..4569)
                     /locus_tag="IVZ67_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8166591.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            5687..>5796
                     /locus_tag="IVZ67_002567"
     CDS             5687..>5796
                     /locus_tag="IVZ67_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8166592.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1943 a          938 c          926 g         1989 t
ORIGIN      
        1 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
       61 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
      121 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
      181 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
      241 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
      301 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
      361 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
      421 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
      481 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
      541 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
      601 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
      661 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
      721 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
      781 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
      841 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
      901 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
      961 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
     1021 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
     1081 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
     1141 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
     1201 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
     1261 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
     1321 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
     1381 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
     1441 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
     1501 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
     1561 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
     1621 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
     1681 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
     1741 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
     1801 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
     1861 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
     1921 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
     1981 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
     2041 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
     2101 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
     2161 ttaattatat agaaagaaca atctttctat agagttagac atgagaggga ctatgcttga
     2221 tttaaaatca acaagcgtat atactgtata tacaaagaaa aaggagggag aagactatgg
     2281 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
     2341 agtttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
     2401 ttcgagcggc aattacaaaa aaacaagcct tatctgatga aattgattta gcaatatctc
     2461 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
     2521 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
     2581 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
     2641 ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta tcctacttat
     2701 acaattgata aagaaacatc cttttcacaa tgcaaataaa cgtactgctt tcttagctac
     2761 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
     2821 tttagcggta tatattgcta cgtatgataa ggatttcgat caactaaaat atgaagtgat
     2881 caacactata aagacacata cgcagcccat ttaaggagtt actgtatgtg tctttatagg
     2941 gttattagct gctatagcga aataattgaa gtaaattaca aatagctata attcaaatcc
     3001 aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
     3061 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
     3121 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
     3181 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
     3241 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
     3301 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt
     3361 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
     3421 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
     3481 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
     3541 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
     3601 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
     3661 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
     3721 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
     3781 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
     3841 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
     3901 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
     3961 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
     4021 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
     4081 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
     4141 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
     4201 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
     4261 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
     4321 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
     4381 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
     4441 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
     4501 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
     4561 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
     4621 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
     4681 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
     4741 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
     4801 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
     4861 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
     4921 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
     4981 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
     5041 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
     5101 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
     5161 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
     5221 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
     5281 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
     5341 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
     5401 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
     5461 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
     5521 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
     5581 atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac tagacagaaa
     5641 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
     5701 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tagctggaaa
     5761 gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DACCZY010000145         5788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCZY010000145 DACCZY010000000
VERSION     DACCZY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.152"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..2197)
                     /gene="copB"
                     /locus_tag="IVZ67_002568"
     CDS             complement(26..2197)
                     /gene="copB"
                     /locus_tag="IVZ67_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8166593.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2217..4403)
                     /locus_tag="IVZ67_002569"
     CDS             complement(2217..4403)
                     /locus_tag="IVZ67_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8166594.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="IVZ67_002570"
     CDS             complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="IVZ67_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8166595.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4625..5065)
                     /locus_tag="IVZ67_002571"
     CDS             complement(4625..5065)
                     /locus_tag="IVZ67_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8166596.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5139..5678)
                     /locus_tag="IVZ67_002572"
     CDS             complement(5139..5678)
                     /locus_tag="IVZ67_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8166597.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1496 a         1302 c         1009 g         1981 t
ORIGIN      
        1 tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta
       61 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      121 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      181 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      241 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      301 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      361 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      421 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      481 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      541 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      601 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      661 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      721 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      781 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      841 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
      901 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
      961 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1021 tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1081 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1141 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1201 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1261 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1321 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1381 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1441 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1501 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1561 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1621 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1681 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1741 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1801 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1861 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     1921 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     1981 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2041 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2101 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2161 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2221 tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc
     2281 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2341 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2401 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2461 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2521 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2581 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2641 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2701 tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca
     2761 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2821 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     2881 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     2941 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3001 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3061 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3121 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3181 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
     3241 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3301 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3361 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3421 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta
     3481 acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga
     3541 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3601 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3661 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3721 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3781 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3841 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     3901 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     3961 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4021 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4081 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4141 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4201 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4261 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4321 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4381 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4441 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4501 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4561 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4621 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4681 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4741 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4801 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga
     4861 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
     4921 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     4981 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5041 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5101 atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
     5161 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5221 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     5281 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc
     5341 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5401 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5461 tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat
     5521 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     5581 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     5641 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
     5701 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
     5761 tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DACCZY010000146         5779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCZY010000146 DACCZY010000000
VERSION     DACCZY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.153"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVZ67_002573"
     CDS             <1..813
                     /locus_tag="IVZ67_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8166598.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVZ67_002574"
     CDS             807..1229
                     /locus_tag="IVZ67_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166599.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="IVZ67_002575"
     CDS             2363..3331
                     /locus_tag="IVZ67_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8166600.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="IVZ67_002576"
     CDS             3341..3829
                     /locus_tag="IVZ67_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8166601.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="IVZ67_002577"
     CDS             4125..4406
                     /locus_tag="IVZ67_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166602.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="IVZ67_002578"
     CDS             4408..4824
                     /locus_tag="IVZ67_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166603.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="IVZ67_002579"
     CDS             5438..>5779
                     /locus_tag="IVZ67_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8166604.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          982 c          947 g         1696 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 acaggggcta cttggcatag tcgacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DACCZY010000147         5744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCZY010000147 DACCZY010000000
VERSION     DACCZY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.154"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(191..712)
                     /locus_tag="IVZ67_002580"
     CDS             complement(191..712)
                     /locus_tag="IVZ67_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8166605.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(715..1557)
                     /locus_tag="IVZ67_002581"
     CDS             complement(715..1557)
                     /locus_tag="IVZ67_002581"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8166606.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(1550..2275)
                     /locus_tag="IVZ67_002582"
     CDS             complement(1550..2275)
                     /locus_tag="IVZ67_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8166607.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            2451..2747
                     /locus_tag="IVZ67_002583"
     CDS             2451..2747
                     /locus_tag="IVZ67_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166608.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            3315..4907
                     /locus_tag="IVZ67_002584"
     CDS             3315..4907
                     /locus_tag="IVZ67_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8166609.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            4920..5660
                     /locus_tag="IVZ67_002585"
     CDS             4920..5660
                     /locus_tag="IVZ67_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8166610.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         1638 a         1127 c         1160 g         1819 t
ORIGIN      
        1 aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat agacatctag
       61 aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat tttttcttat
      121 ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat aaaaagtagc
      181 agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt ttgatgtttt
      241 tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct gttaattcgg
      301 ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata tcttgtggcg
      361 ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca attacatcaa
      421 tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga tcttttccat
      481 gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg aggatataac
      541 ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata tcgcctacaa
      601 tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact ggtttttctg
      661 tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc acacttattc
      721 ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat acgtgtctgt
      781 tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca atacttgctg
      841 ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc ctattttagt
      901 taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca tatcgatctt
      961 atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct tgatgatacg
     1021 agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa ccgaacctgc
     1081 gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc ctccgcctaa
     1141 ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt tacgtttcac
     1201 tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca tgcagttatc
     1261 accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat aaaaattctc
     1321 cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat agattttttt
     1381 ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg attctgcttg
     1441 gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct caggatcatt
     1501 ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt cagtcataat
     1561 ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga ataaacgtat
     1621 aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct gacggctggc
     1681 gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca atgacgatca
     1741 gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga aacccttcac
     1801 catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg tcgctgtgaa
     1861 gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga tacagactcc
     1921 catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg atgttttggg
     1981 aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga atgatattgg
     2041 gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc ggttcattca
     2101 ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag attcctgaca
     2161 aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc aaaaatgtaa
     2221 tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt ttcatgtttt
     2281 gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa aagtcagaaa
     2341 aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt cactgacaaa
     2401 agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat atgttaccat
     2461 tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta agcgtttatt
     2521 tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt gctttattca
     2581 acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca tggggctggg
     2641 gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc gtcgttgttc
     2701 ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg atcgagatgg
     2761 aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca tacatttttg
     2821 tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt gttttttcgg
     2881 taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt tatagattga
     2941 caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg agatgcctca
     3001 atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa acatggagaa
     3061 gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta tgttgggacg
     3121 caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat gttttgtgag
     3181 aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg gtcgcttttt
     3241 attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc atataatcag
     3301 aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt gcttttgtct
     3361 tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg agcgatgagt
     3421 ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa acagatgaga
     3481 ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat gttcaaatcg
     3541 ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg aaatgggatg
     3601 gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga atgagcccta
     3661 ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca caattagtag
     3721 tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat ctttctggag
     3781 caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag ataccagatg
     3841 tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta gcttctggaa
     3901 tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag
     3961 acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg gaagatgttc
     4021 agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac cagattcttt
     4081 ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg
     4141 ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg atttggaaac
     4201 aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca ttgatcggga
     4261 cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta cctgaatcag
     4321 gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct atctatgtcg
     4381 aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac ggtctggcac
     4441 tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc tctctcaata
     4501 ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat gaagggcagt
     4561 tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa gttgttattc
     4621 cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc aatatcaaag
     4681 atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga aactctgctt
     4741 ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc tacttagtca
     4801 tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag
     4861 cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga ggaagaatca
     4921 tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa aatgaagtat
     4981 tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc ggttcttccg
     5041 gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca acaggcggcg
     5101 agatcattta taatggagaa aatgtattag ctcctaaata taatttacct aaatatcgaa
     5161 cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat gtcttggaaa
     5221 actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc aaaaaagtag
     5281 caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa ccagcacaat
     5341 tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg
     5401 tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc gaagtgctta
     5461 atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg catgagatgg
     5521 aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg attgcagaag
     5581 aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa gaattccttt
     5641 cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc ggtgtttacc
     5701 gcagttcttt tttatgaatt actcataaaa ggtaaatttc tgaa
//
LOCUS       DACCZY010000148         5666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCZY010000148 DACCZY010000000
VERSION     DACCZY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.155"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..454
                     /locus_tag="IVZ67_002586"
     CDS             <1..454
                     /locus_tag="IVZ67_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8166611.1"
                     /translation="KELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQL
                     KANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSL
                     NDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            735..1955
                     /locus_tag="IVZ67_002587"
     CDS             735..1955
                     /locus_tag="IVZ67_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8166612.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1952..4672
                     /locus_tag="IVZ67_002588"
     CDS             1952..4672
                     /locus_tag="IVZ67_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8166613.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4656..5600
                     /locus_tag="IVZ67_002589"
     CDS             4656..5600
                     /locus_tag="IVZ67_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8166614.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2107 a          908 c         1113 g         1538 t
ORIGIN      
        1 caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa
       61 tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc
      121 taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt
      181 tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt
      241 gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga
      301 tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag
      361 tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt
      421 atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag
      481 aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc
      541 aaacgaagcg aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt
      601 ttttcgaata aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg
      661 aacaaatttt cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa
      721 ggaggaaatt tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat
      781 ttgaaggact gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga
      841 ccgttttatc aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg
      901 caggagataa ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac
      961 agatgaaacg gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt
     1021 tctatcaaaa agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg
     1081 aaacggtaga gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat
     1141 ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac
     1201 tcacagatta ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact
     1261 atgctccttt ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc
     1321 atatccatgt gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc
     1381 aaggtcatac taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag
     1441 gaagttgcca aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat
     1501 cattgaggaa agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa
     1561 aagtagagga tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata
     1621 cagatgtttt ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct
     1681 cagataggat aaatgatttc tttatttggc gtatttctct catagagaat actttttata
     1741 cgaagacccc acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga
     1801 cacctcaaga tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa
     1861 tattgagcga actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta
     1921 atcaagattt tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc
     1981 ttttggaaaa tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg
     2041 gggaaacgaa gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt
     2101 cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg
     2161 caaaatatgg cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca
     2221 aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga
     2281 aaaactgatc gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga
     2341 gcagttgatg gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt
     2401 aggcatcacg ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca
     2461 acaattattc aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa
     2521 agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat
     2581 ccaacaagaa caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc
     2641 gaacaaacaa ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg
     2701 gcaatctctc aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct
     2761 ggagtctttc tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac
     2821 actggaaaag catagcggga tggatcagca gtcagccaga tattattttt atttagagca
     2881 ggagcaggaa ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga
     2941 atggcaaaga ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca
     3001 aaggtggcat tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa
     3061 gcatcttctg gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct
     3121 ccaaatagct aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac
     3181 ctatccagca ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta
     3241 tatgctgatg gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa
     3301 agtgatcctt ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc
     3361 aaaaattaaa actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg
     3421 acaacttgat ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg
     3481 agaagaaaaa cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg
     3541 aaagatggat acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa
     3601 attatatggc gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg
     3661 acaaatcgac caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat
     3721 ttcagaaaaa atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc
     3781 cagaagctat cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca
     3841 aaaagtatgg atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc
     3901 ggaagtacct gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa
     3961 tgaattatcg gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc
     4021 tgtaagactg cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc
     4081 aagtgaaaat cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt
     4141 ggacgaactt tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga
     4201 atggggagca cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga
     4261 aagacaatta ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg
     4321 aaattatcag aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga
     4381 atttcccatc tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt
     4441 tgcttacctc tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg
     4501 gcttcattat gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga
     4561 aaagtatcaa ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat
     4621 gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa
     4681 tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa
     4741 acggaaaaaa atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat
     4801 actgggatgc ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa
     4861 acggtaaaag agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa
     4921 caagggtaga tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag
     4981 aagaaatgga agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc
     5041 gtattgttcg ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa
     5101 aaagaaacca tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact
     5161 tagggaaatc aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta
     5221 tcattttgca tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata
     5281 cgcttgcagg aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct
     5341 gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct cgtattacgt catatggttt
     5401 tagcacacca tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag
     5461 aaatattaca tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc
     5521 gtcaaacaga accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct
     5581 atgtaccaaa agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga
     5641 cataagtctt cgcttttttt gttttc
//
LOCUS       DACCZY010000149         5417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCZY010000149 DACCZY010000000
VERSION     DACCZY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.156"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..407)
                     /locus_tag="IVZ67_002590"
     CDS             complement(36..407)
                     /locus_tag="IVZ67_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8166615.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            622..807
                     /locus_tag="IVZ67_002591"
     CDS             622..807
                     /locus_tag="IVZ67_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166616.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(890..1078)
                     /locus_tag="IVZ67_002592"
     CDS             complement(890..1078)
                     /locus_tag="IVZ67_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166617.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(1102..1365)
                     /locus_tag="IVZ67_002593"
     CDS             complement(1102..1365)
                     /locus_tag="IVZ67_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166618.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            1523..1780
                     /locus_tag="IVZ67_002594"
     CDS             1523..1780
                     /locus_tag="IVZ67_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8166619.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(1851..2042)
                     /locus_tag="IVZ67_002595"
     CDS             complement(1851..2042)
                     /locus_tag="IVZ67_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166620.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2183..2503)
                     /locus_tag="IVZ67_002596"
     CDS             complement(2183..2503)
                     /locus_tag="IVZ67_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166621.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(2526..2960)
                     /locus_tag="IVZ67_002597"
     CDS             complement(2526..2960)
                     /locus_tag="IVZ67_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8166622.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            3295..3933
                     /locus_tag="IVZ67_002598"
     CDS             3295..3933
                     /locus_tag="IVZ67_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8166623.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(4257..4340)
                     /locus_tag="IVZ67_002599"
     tRNA            complement(4257..4340)
                     /locus_tag="IVZ67_002599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4304..4306),aa:Leu,seq:cag)
     gene            4868..5368
                     /locus_tag="IVZ67_002600"
     CDS             4868..5368
                     /locus_tag="IVZ67_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8166624.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT         1610 a         1065 c          828 g         1914 t
ORIGIN      
        1 ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc taatgctttc
       61 tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc tttattgcta
      121 attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac cgttcgttct
      181 ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc atttacttcc
      241 tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt tagctgatca
      301 agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc aaatagcaaa
      361 ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt tctacctcct
      421 tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa gttggagatt
      481 tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat cgttatttga
      541 tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa aatcatgttg
      601 atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt caaggaaatg
      661 attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa agaacaatta
      721 ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga cagtgtcaaa
      781 aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa tcaaaggagg
      841 acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac taaaataaac
      901 gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc accacatatt
      961 tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct ctttttacat
     1021 caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat ggattcattt
     1081 ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg cttttttggt
     1141 atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg ggatactata
     1201 ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa taccatttat
     1261 tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa tcacccgaat
     1321 attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa tcccactcct
     1381 tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac agattttacc
     1441 ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg tatcatggac
     1501 tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt atattccttt
     1561 ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga agttttcagt
     1621 tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt ttaggcattg
     1681 ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag taatttttct
     1741 tattatttgg atctattttt tatataagaa tcagaattga atccctcgca tgtttaaaaa
     1801 gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt ctattctgtt
     1861 ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc tataaattct
     1921 tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag gcttttcgct
     1981 tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt tcctgcttcc
     2041 atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta tacgcattat
     2101 tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat cagttttcta
     2161 aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct tctgttaatt
     2221 tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat aattccataa
     2281 atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca gggaatcctt
     2341 tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct ttccacttta
     2401 ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt gctaaaattt
     2461 ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat tcctccctta
     2521 tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata agaagcggtc
     2581 gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac gatcaaatca
     2641 atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc agtttcaact
     2701 attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt cagcatggct
     2761 acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc actatgggtc
     2821 agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc ctttgctaag
     2881 tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc taccatgatg
     2941 ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa catctcaagc
     3001 aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc aatttctttc
     3061 aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat tatttctgca
     3121 ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt acaaaaaacg
     3181 tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac gtgttaaagt
     3241 attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt tttcatgaca
     3301 tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt cttcatggga
     3361 acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct atcaacgatt
     3421 tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc aaattcaatt
     3481 tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga aggctctgca
     3541 caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc tgaacctgtt
     3601 caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc agcgcctgtt
     3661 gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc aagtacaagc
     3721 tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc aacgaacgga
     3781 cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta ctctgctgca
     3841 aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg ggaagcggcg
     3901 aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg tttaaacaca
     3961 ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt tttgagttta
     4021 agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag aatctataaa
     4081 tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac aaattttttt
     4141 tgatttattt gaatctggtt ccgttgttta aaatttttcc tatattcttt tagataatat
     4201 gttattttct ttctcatcgc tccaggtgta ccttctcttt ataaaaaaac aaattatgcg
     4261 gctaagaaga ctcgaacttc cacgaggtca ccctcactag cgcctgaagc tagcgcgtct
     4321 gccaattccg ccatagccgc atcacatctt ttagtttagg attacatgtg ttttttgtca
     4381 agaacatatt tttgttcttt ttcatttctg tacgccctcc cttatgccat ctacacaaat
     4441 aattcatccg agcaactcca tcatctattg agggttggtg aaaaaatctg ttttatccat
     4501 atgatcgatt tcaatgttaa acgaacgact ctgggattag taactatttt atttttttct
     4561 tcatttcttt tttcctcttt ttctcgattc agctatcttc tttcatttgg atattgatta
     4621 tttcttctgt tgattactat ccttctggaa ttctagacgc tgaagtaatt cacgttttct
     4681 ttcttctaga gatcataagt aacgaatgaa cgaacttcaa ataatttttt tattgtaatt
     4741 catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa aaataggcat
     4801 ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac ttaaagaggt
     4861 gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt atcagctaca
     4921 aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct atacatttgc
     4981 tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg atcttcctga
     5041 gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt atgtaggaat
     5101 ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc atattggtta
     5161 cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag cattagtact
     5221 acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata aaaaaaatct
     5281 tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag atgagaaaga
     5341 atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa agggatttga
     5401 atgtccttta tcaatag
//
LOCUS       DACCZY010000150         5293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCZY010000150 DACCZY010000000
VERSION     DACCZY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.157"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..253
                     /locus_tag="IVZ67_002601"
     CDS             <1..253
                     /locus_tag="IVZ67_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8166625.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVZ67_002602"
     CDS             complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVZ67_002602"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8166626.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1620..2456)
                     /locus_tag="IVZ67_002603"
     CDS             complement(1620..2456)
                     /locus_tag="IVZ67_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166627.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2640..3857)
                     /locus_tag="IVZ67_002604"
     CDS             complement(2640..3857)
                     /locus_tag="IVZ67_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8166628.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            4240..4323
                     /locus_tag="IVZ67_002605"
     tRNA            4240..4323
                     /locus_tag="IVZ67_002605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4274..4276,aa:Leu,seq:gag)
     gene            complement(4563..5293)
                     /locus_tag="IVZ67_002606"
                     /pseudo
     CDS             complement(4563..5293)
                     /locus_tag="IVZ67_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1626 a         1100 c          858 g         1709 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcca aaaaacctta aaaccaaact
      361 taagaagttt agttttaagg ttaacttaaa ttatattatg cgtggaaacg ttccacgcca
      421 tctagataag ttgcttccaa tgtcatatca gggttcaata cgataaagtc tgcatcgcgt
      481 ccttgagcga tttttccaca tgtatcatcg attttacagc tgattgctgg aatcagacta
      541 gccatcatga ctgcttgttc tggtgttgct aatccccagt ctacaacatt tttaattgct
      601 tctttcaatt tcaatatact tccagctaag ttgccagatt ctagacgtgc tgttccttct
      661 tttactacta ctggaaattc gcctaaattg taatttccat caggcatccc accggccatc
      721 atacagtctg taattaatgc gacatgatca tgtcctgctt tttccattag gattcccgca
      781 gcatttggat gtacatgatg gccatcacaa atcaattcag aaaagacatg ttctaaagat
      841 aataacgcac ccaccatacc tggttcgcga tgattcaatc cacgcatacc attgaaagca
      901 tggacaaata cacttgcgcc tgcttcaaca gctttttgcg cttcttctag tgtagcatta
      961 ctgtgtccta gagaaacaac tactccttct tcagtcactt gtttgacaaa ctcttctaca
     1021 ccttgacgtt ccggtgctaa agcaattttt ttgattaaac cacctgaagc ttcttgccat
     1081 tcatggaaag tatccaaatc aggatccccg aaataacttg ggttttgtgc ccctttgtgt
     1141 tcttctgtga agaaaggacc ttcaaaatag attcctttga ttttagctcc tggtgcttcc
     1201 tgatacattt tgccgattgt ctcagctaca tcttttaaac gttctttgct tgacgtcaat
     1261 gttgttggta aaaatgaagt cactccacaa gataacagac cttcagacat cactttgatg
     1321 ccttctgcat cattatccat tacatcatga ttcatatagc cgtgaatatg tgtatctacc
     1381 aaaccaggag caatccattt gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt
     1441 tctttggtgt attcaccaaa aagtccatcg gtaatttcaa gatagcctgc atgtttgaca
     1501 cctgacgcta aaaagaattt gtcagcatag ataaaagttc tcattctatt ctctcccttt
     1561 gtttttctac ctctaaagta ctcttattta gagaagaagt caagaatggt ctacaccatt
     1621 tacaaatttt ttgaaatttg tttcacagct tgtgctaagc cttcatagaa tgctgctctt
     1681 tctggttctt ctttacgctt gtatattaag cttttaagca caaataaatc attgaggtaa
     1741 tagtagctat tcttctcttg agcccaatag attccttgat tcacctgttc taacgcttcg
     1801 tcgatttcat tttgatggaa taagaaattt ccataattat aaaaaatttg cgaaaattca
     1861 ctttttgatc gttcatcagc tgattgatgc agcagttgga tgcttcgact caaatagtag
     1921 cgtgcttcag ccgtttttcc tgccacacca tatatttttc cgatacaact aatcagttgt
     1981 atttctgtgt cagaaacatg gattctttct ttttggctag agtagcttaa tgcttctttc
     2041 aaataataaa gggactgaga gatattttga cgcaacgtga attcgcaaac accgagataa
     2101 taataatagc gttgaaagtc agttgcttga tacagatttt cagaaatatc agaagatttt
     2161 agtacttgtt ctaacttccg ataatcacgt ttttggaaac aactatccaa cagttcaaat
     2221 aatttatcgt ttgtccgagt agtttttcga gagagattca tcacatgatc aatcgttacc
     2281 cctagccgtt gacaaatctg catcatcacc aatacattcg gcagttcctc attattttcg
     2341 atccgactta atacactttg tgaacaaata tcatgcgaaa gcattttttg cgtcattttt
     2401 tgttcttttc gaatctcttt aatgatcgtt ccaaaggact cgattaacgt ttccataagt
     2461 atcctcccca aaatatgcgt attcgcattc tattcgtatt ttactttagg aagtcgcaaa
     2521 cttcaagaaa agacttgcaa ctaaataata tactttagtt atttaagaaa actccactag
     2581 aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat
     2641 catttttggc gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca
     2701 tccattttag gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca
     2761 ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc
     2821 aaaatattga atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt
     2881 acggctgaaa gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag
     2941 atccttccgc ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg
     3001 ataatttgct gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct
     3061 tctatatagc ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat
     3121 cctgctacac gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct
     3181 aatgtagcaa tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg
     3241 gaataaataa tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt
     3301 ggataaagtg ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc
     3361 gttatttttg atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata
     3421 taatgaacag gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc
     3481 attggaacca gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat
     3541 tcgctcactc cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt
     3601 tggcaatatt gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct
     3661 tctgagtcac gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca
     3721 aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc
     3781 tccgcttctt ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat
     3841 ttttcaaatt ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa
     3901 ttttttgtta tttactattt caatatcata ctccatccta gaaaattttg ccacttattt
     3961 aggaaaaacg taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata
     4021 gtcattccat agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa
     4081 accaagcaaa aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc
     4141 tgaaaattct ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa
     4201 ctatgtaaaa cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca
     4261 gacaagcatg attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct
     4321 gcactacaac tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta
     4381 tgccattata tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc
     4441 ctgaggtgag tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa
     4501 caattaatcg aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta
     4561 tattatctgt ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat
     4621 tgcataaatt catttttttg tacactcatg cttttcatta gctctttaac tgccattagt
     4681 actaagatgg cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact
     4741 gtgggagaaa atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa
     4801 aaaatagaag ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat
     4861 aatgccaaga ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt
     4921 cctaactctg taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt
     4981 ccattgacgt aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag
     5041 aaaaacggta atgcagtcaa tatacgcaca taatagtttt gacatttcct atatgttaga
     5101 aggaaaatta agaaaaatag caataaaaat aagatattca tgtctaaaaa aacggctttc
     5161 caaatgatga aatacctaaa tctaacttat taagaaagtt gaaattttta tattcaggaa
     5221 accatttatt aatttcatgt gcagcacgta atgcattccc tggagttgtc aaagagaaaa
     5281 ttaattctgc taa
//
LOCUS       DACCZY010000151         5277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCZY010000151 DACCZY010000000
VERSION     DACCZY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.158"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            17..373
                     /locus_tag="IVZ67_002607"
     CDS             17..373
                     /locus_tag="IVZ67_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166629.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            389..739
                     /locus_tag="IVZ67_002608"
     CDS             389..739
                     /locus_tag="IVZ67_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP8166630.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            768..1547
                     /locus_tag="IVZ67_002609"
     CDS             768..1547
                     /locus_tag="IVZ67_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP8166631.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1558..2220
                     /locus_tag="IVZ67_002610"
     CDS             1558..2220
                     /locus_tag="IVZ67_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP8166632.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2239..3345
                     /locus_tag="IVZ67_002611"
     CDS             2239..3345
                     /locus_tag="IVZ67_002611"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP8166633.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3335..4426
                     /locus_tag="IVZ67_002612"
     CDS             3335..4426
                     /locus_tag="IVZ67_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP8166634.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4439..5179
                     /locus_tag="IVZ67_002613"
     CDS             4439..5179
                     /locus_tag="IVZ67_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP8166635.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1694 a          864 c         1258 g         1461 t
ORIGIN      
        1 cgaggaggaa acaatcatga ttaaagtagt tattgcagac cgtatgggta aaggacaaaa
       61 tgtggccaaa ggggtagaag cagctggtgg aaaagcagtt gtcgtaccag gaatgggggc
      121 agatatgcgt ttgggcgatg tgatgcaaca agagcatgca gatatgggta tctctttttg
      181 tggtagtggt ggtgcaggtg cattaacagc agcaacaaaa tatggctatc cagaacgaca
      241 tggtatgcgt tcgattgaag aagggatcac agctatcaag gatggcaaaa ctgttctagg
      301 tttcggcttt atggatcaag aggaattagg gaaacgtctg acagaagaat ttatcaaaaa
      361 gcatggaaaa tagaaagagg gacatgaaat gagcgttcaa aagacaattc agcaaaaagt
      421 tatcgtctcc ggtaaagggg aaacaaagca acatgctttt gcatcagcgt tagcagaaat
      481 tcaaaacaaa ataatggaac aatcaaacgt attattgcga atcgaaccag tcaatgtgga
      541 tattttaaag gcggtcgaaa acaaatacac agaacgcttt ctatttttct tcttcccacg
      601 gcaacgtatt agctatgaag tcgaactctc tgttactgtg gctgtaacag aaataaaatt
      661 ggcagacata tcgttcagcc agcaaatgac tgaagatcca aatgggattg ccattccatt
      721 tgtaactaaa aaaatctaat tttgaatcat aagggggaaa gaataacgtg gatttcgtaa
      781 tcattctagt caaatcggtc attatcgggg gattacttgg ttttgcagca tctgtcgggg
      841 cagctcgaat gtttcatgca cctaatacac aaggtttagg agcttttaga acattaggcg
      901 aaatgaatgc ctgcatgggt gatccagctt ctcatttttc atttggttta ggctttttct
      961 ttaatgcatg ggcttcttca gttggtgctg gtgcattcac acaagatgtg acgcatcgaa
     1021 tcattcccaa ttgggcagcg gcagcacttc tagcaaaaaa caaagatgtc acccagacca
     1081 tgcatgatcc gaaaaaaatg gggatcattg gagcggttat cggggcagtg gtggtagcct
     1141 tcttaaacac aacagctgca gcaattccag aggcgttaca agtgacggcg gtttccgttt
     1201 tggtaccagc agcgacgatt ctgatcaata cagtaatgcc agttattttt tggttggcag
     1261 ctttagacgc agggaaacga actggttttt ggggcactct ttttggtggt atcgcgcaat
     1321 tgattatggg aaatgcggta cctggtgttg ttctaggaat cttgattggt aaaggcgtcg
     1381 atgagagcgg ttggaatcgc gtcacgaaga gcatgatgat cgctattgtt ttattattta
     1441 ttttcagtgg atttttccgt ggatttgata tggaaatgat caagagcttc cagcttcaag
     1501 tgcctaattg gcttgataac ttgcacaaca tctttagtgt gaaataggag gagaatcatg
     1561 gagacaacag aagaacaagc aaaagtatta gaagcaaagc ggcaaaagag cttttggttt
     1621 gcagactggt cttttccgct aatcgtagga attatgtccg ctgctgtatt tgcaggaaca
     1681 cacatgtatg tgatgtatgg tgtgggtgca ttcaatgaag tttcgatcgt ggcgatgtta
     1741 aaagccgggc tggatggcgg atcctatggc gcagcggcag cgtttggtgc tagtttctta
     1801 tttgccagaa ttttagaagg ctctttagtt gggatcttgg acttaggcgg ttcgatccta
     1861 actgggatcg gaattggggt accagcaatt ctcttaagta tgaaaatagc ggcaccaatc
     1921 gataattttg cactctcttt attgactggt ttggttctag gtttgattgt tggtggcatc
     1981 attgtcttga ttcgtaaatt tacgatcaat caatcaaatt ccacttttgg tgctgacgtc
     2041 atgatggggg caggaaattc atctggacgt tttttgggtc cattaattat attaagcgcg
     2101 tgtgcagcat ctattccaat cggcattggc gctacattag gagcactagg attctatgct
     2161 tggaaaaaac caattgctgg aggtgctatt ttaggagcaa tcttcccggc agtaaattaa
     2221 tagaaagagg gtcattaaat gtttgatact ttgattaaaa atggaaggtt agttgatgga
     2281 tctaaaatag aggtcgctat agaaaaaggc atgattaaag cagttgcagc agaaataaat
     2341 cagccggcgc aagaagtagt tgacttggag ggaaagtatt atttatccgc tgggtggatc
     2401 gatgatcatg tccactgcta tgaaaagatg aatctttatt atgactatcc tgatcagata
     2461 ggggtcgaaa aaggtgtgac gacggtcatt gatgctggaa ctacgggagc agaaaacatc
     2521 cgtgaatttt atcaattgac gaaatcggtc aagacaaatg tgtatgcgct aatgaatatc
     2581 tctaaatggg gcattgtcaa acaagatgaa cttgctgatc taacaaagat tcaagaagat
     2641 ctagtaagta ctgcgttagc agaactgccg gaatttattg tcgggattaa agcgagaatg
     2701 agtaagacag ttgttggtga aaatggcatt aagccattag aattagctaa aaaaatccag
     2761 acagaaaaca atgatctgcc attgatggtt catatcggtt ctgcacctcc tgagttagaa
     2821 gaagtattgt caagaatgga aagaggcgat gtattgaccc attgttttaa tggaaaacca
     2881 aacggtattt tagatccaaa gacagatcaa attaaaaact ttgtctggga tgcttacaat
     2941 aatggtgtcg tctttgatat tggacatggg acggatagct ttaatttcca tgtggcagaa
     3001 accgcactaa aagcaggaat gaaagctacc tctattagta cggatatcta catccgcaat
     3061 cgtacaaatg gcccagtttt tgatctagca actactttag aaaaattgag ggtagtcggc
     3121 tattcttggg aagaaatcat tgaaaaagtt acagcagttc ctgcacggaa ttttcatttg
     3181 aagaataagg gccagttagc tgtcaattat gatgcagatt tgacgatatt tgagattgtt
     3241 gaaaatgaaa aggaattgaa agattccaac ggatttacac gtatagctaa agaacaaatt
     3301 ttaccgatta aaacaattat tggaggcgtc atatatgaca attagctatg gaaagtttga
     3361 tttaaaagaa gtcattaatg catcgggaaa aatgacgatc ctaggggttt ctaaggtatc
     3421 tgaaagtgtc ctagcagcac aacgatttgg aggcgagcat ttttttgaga tgagtgatct
     3481 tgccatcaaa accggcgctt atctttctca attactgaaa gtggaagatg cgcaaatcgt
     3541 ttcttctgct tcagcaggga ttgctcaatc tgttgctgct ttaatcggac agggcgacat
     3601 gtaccatgtc tatcatccct atacagaaaa aatcgctaga cgggaaatca tcatacctaa
     3661 gggacataat gtagattatg ggacaccggt tgaagtaatg gttgctcaag gtggaggaaa
     3721 agtcatagaa gctggctatg caaatatgtg tacaccggaa catgtttcca tgatgattac
     3781 ggaaaaaact gctgcgcttc tatatattaa aagtcatcat gcagttcaga aaagtatgct
     3841 gacggtggca gaaatggtag aagttgccaa agctcatcgt ttgccattga tcgttgatgc
     3901 agctgcggaa gaagatttat tcaaatatag cgaaatgggc gttgacttag tgatttactc
     3961 tggggcaaaa gcaatcgaag gtccgagtgc cggactggtc ataggaaaaa agaagtatat
     4021 tcaatggatt cggctacagt ctaaaggtat tggccgagca atgaaaatcg gtaaagataa
     4081 tattttaggc tttacccagg cactagaaga ttatttaaaa aatgggagcg aatccggtga
     4141 ctccatgaag caacgattag cgccttttgt taacgcaatc aatcaacttc ctgacctttc
     4201 agcgaaaata gttcaagatg gtgccggcag agatatttat cgagcaagtg taacgattaa
     4261 tggcgataaa agtgcgaaag aagtcattca ggaattaaaa gcacgaaatc cagcagtcta
     4321 cacccgagaa tatcaagcaa ataacggaat cattgagttc gacattcgtt ccgtcagcca
     4381 aaacgaaatg gaaaaaatta taaaaagatt gagagaaatc atgtaggagg cttttaaatt
     4441 gtcagttatc ccaaattatt ataaaaatcg tgtatgttta aatgtattag ctcattcagt
     4501 tgaaaatgca aaagcatgct atgaagcagc ggaagggcat gtcgtactag gtgtgctatc
     4561 gaaaaattat gctagcgatg aagcagcaat cgaagatatg aaaaggtacc aagacgcaac
     4621 caataatgcg ttgtcagttg gattaggcgc tggagatcca aaccaaagtc agatggtttc
     4681 gcgtttatct ggggttttac aaccacaaca tgtcaatcaa gtatttactg gcgtggggac
     4741 ttctcgtgct ttattgggcc aaaatgatac agtcatcaat ggattggttt caccaacagg
     4801 taagatcggc tatgtcaata ttgctacagg tccgttaagt tcacaagcgc cagcagctga
     4861 agtgcctatt gaaactgcca ttcaaatgct gcaagacatg ggaggtactt caatcaagta
     4921 tttcccgatg aaaggcttag cacataggga agaatttaaa gcagttgcgg aagcttgcgc
     4981 aaaatatgaa ttctatttag agccaacagg aggaattgat ctttcaaact ttgaagaaat
     5041 tctgaaaatt gcaatcgatg caggtgtaaa aaaagtgatt cctcatgttt atagttcaat
     5101 tatagataaa gaaacaggag atacgcgtat aggagacgta aagatactat taagcatggt
     5161 aaaaaaaatt attcactaac tcattagaaa ataagaagct tattccagaa tgaaattaaa
     5221 tggaataagc tttttataat taattacgga aattgttaat gtagataata atttaga
//
LOCUS       DACCZY010000152         5180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCZY010000152 DACCZY010000000
VERSION     DACCZY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.159"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            107..406
                     /locus_tag="IVZ67_002614"
     CDS             107..406
                     /locus_tag="IVZ67_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166636.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            671..1546
                     /locus_tag="IVZ67_002615"
     CDS             671..1546
                     /locus_tag="IVZ67_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166637.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1543..1761
                     /locus_tag="IVZ67_002616"
     CDS             1543..1761
                     /locus_tag="IVZ67_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166638.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1748..4375
                     /locus_tag="IVZ67_002617"
     CDS             1748..4375
                     /locus_tag="IVZ67_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166639.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4390..4608
                     /locus_tag="IVZ67_002618"
     CDS             4390..4608
                     /locus_tag="IVZ67_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166640.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2047 a          640 c         1017 g         1476 t
ORIGIN      
        1 attcttataa atacaaataa atacattttt ttacgcaatt tgtatttatt tgtacttata
       61 aaggtagagg agttatagaa taattaatta aaaaggagag gtttttgtgg aagcaataaa
      121 attaatgatt tcagaagaac aggaggaaat gtttctaaag cagttttatc aaattgctac
      181 aaaagctatt gaaaaggctc aagaaaatgc ggggatgaca agggaatatc tcaatcaaaa
      241 agaaatggcg gaatacttaa atgtgagtga aaacttttta aaaaatgctg tgagaaatga
      301 gggattgccc gtagttgaat taggggcaag aaaattttat tcgaaaaagg cagttaatga
      361 gtttatgttt gctcgaatga aaagagggga aaatattgga agatgatgaa gtagaaatta
      421 taaaaaaatg ctttaaaaaa agaaaaaaag tgttcccaaa agtttgcaga ctgggggaac
      481 actcaataat aaagtgctga gaaaacttta tttatagcta ttatatcaaa aaagaaggtg
      541 aaaaggtgtt ttcttctgga aatgttgtag ttgacaaact tagagaaata aattttgacg
      601 gaaatgtaat ccacacacat ggtacgatca attaagaaaa aaaacgaaaa aaggtgttga
      661 gaagccctat ttgaatgcaa tagttatctt agcagaaatt acctattggt atagacctaa
      721 aaaaattttt aacgatgatg gtcagctact tggttatcaa aaaaagtttc ttgaagatat
      781 tttgcaaaaa tcatataagc aattaagcaa aaaaacagga ttatcagaac gagctataaa
      841 agatgcaatt gtgtacttag agaaaaaggg gattatcaaa agggttttta gaaatattta
      901 ttatgagaaa agagcagtta cattatcaaa tgttatgtac attgttttaa atgtacataa
      961 attgatagaa attacttttc ctactttgga ttgcgaaaaa aaggagtata tgccgaaaat
     1021 tcgtcagagg ggtatcgaaa aaaagagata tgtctcttta aaaaaaggta gaggtatgta
     1081 caaatttgta tatacgtata caaagactac ttcacagaac atatcaaaga ctactctcgt
     1141 tatggaagaa gaggatatta atataaagag gatttacagt aagattagtg ttctttatga
     1201 cgcaaataac gaagatggtc gaaaattatt tcaagtgtat cactatttga gaaacgctaa
     1261 tgtagataca gaagatatca gaaaaatcat tgaatattta tctgaaaata ctcagtatct
     1321 tattgctgag tgtattataa aacaacatga agcatgcttt tttcaaagaa aaactgaaga
     1381 agggttatac gattatgtaa catatttttt gaatggactt cagaaaagat ataaaagtca
     1441 atttttagaa aatgtagagc ctatagatca gttcaaaaaa gctttcgaaa tcaaagagtt
     1501 acctgaaatt actatgcata attggttgaa aggagaagaa tcatgattaa aaatcgctta
     1561 atagatcaaa tgtggtgtct tgatcaaaat acagatactc tcgaattaat cgagagtatc
     1621 tgcttcaaca cagtagttct cgatttaaga gctgaaaatg acaattgtgt aactcatatc
     1681 ataattaatc aaaaaatggc tcagcagctc tctgagagcc taagaaaatg ggctgaagga
     1741 gaaaactatg aaagcaatta aagaaaaaat cgttagtaga aaaataaact ggttagcact
     1801 cagtgtactt gttttaatga ttagcgttgt ttttacaagt aatattgttg aagctagcat
     1861 tttcaattgg tttgatgaca caaaaagtca aacagagttt ttgaataatt ctctttatag
     1921 accttaccta aaaaaataca atggagattt ccttagtcag ttcgggattt ggcttggatg
     1981 ggctgtggta aaaggcatgt ttactgtgac tgattcgatt caaaatatga ttccagatgt
     2041 gttggatttg ttcaatttca ttgaaagtac agggctaaat aatgtttatc aatcagtcat
     2101 aaatacaatt gtcgttggat taatgatcct ttcattgatg tttgttggct acaagatgat
     2161 tactggaaaa ggaacaatag atttaagatc tgttggaatg aatatagtga tgtctgtagc
     2221 gttaattctt ttaatgccaa caatgatttc tagcggtatt caatttgcca aaatatttta
     2281 caatgatgct actacgatta caaattcaga tgacggagtc gcttggtcat tgataaaaca
     2341 aggagtaaca gatctggcat atattaataa aacagatcaa tactcaagca ttgataaaac
     2401 ggaggatcga aataagttaa cacgaaaaaa ttttcaacag acagatttaa cacaagtgtt
     2461 gacggataaa gtgattgata aattggaaaa agaaaatcca gctgcggata atcttcgcta
     2521 tgaattagtt gaaaattcta ataatgaatt tgttgccaca aagttttctg ataacttctt
     2581 atcaacattt tctgatagct tgaaatcagg ttattatcga tatcaagcaa atctttgggg
     2641 gatttcgatt gggttgacag cgttggcaat cgcctatgtg ttcagcgcgt ttgtgatcat
     2701 tacagcaata ttagaacttg catttaaacg tgtgttaggt gttcttgttt ttgcgacaga
     2761 tattgaaaca ggacaacgtt caaaagttgt tttatccgat attttacaat gttacttaac
     2821 agttggattt caagggtttg gactatctat gtttgctatg tttatcaatt ttttaaatgc
     2881 tggacaaggt atctccacaa atatttttat aaaaacaatt gcttatatct gtgctgtgtt
     2941 tgtgctgata aaaggcagtg gaacagtcat gagatacttt ggtgttgata ttggactcaa
     3001 agaaggctat gggcaacttg cttctgcttt tggtatgggc gcaatgctgt ttagaaaagg
     3061 atcaactgga tttaatcgtg ctaaaggaag cggaaatgga aatggatcag acagtggaga
     3121 aggagaagat cgaaaaccag aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa
     3181 aactggaaga gctttgggat atgcccatga acgtggatta tcaggtttag cttcagatgg
     3241 agctacaatg gctagtgaaa gagcaataaa accatttaag agtatgcgtg atatggctaa
     3301 tgataccaag aataagttca aagaaggttt agatgatggt acagtgtccg ctattaataa
     3361 aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat atgctgattc
     3421 catgccaaca cgtctttcgg atagaaaaga tggtgctaag gttgaaaatg ccgatagaat
     3481 tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa
     3541 tcctgtaagt gcaattcagc aaaaagttca acaagatatt gaagaacgta aaaatgctat
     3601 gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgacaag aagctaaata
     3661 cacgcctgaa gcaatgaatc gtgaggaaat gttaaggaaa cgtgtagaag ggcgtacagg
     3721 agctaatacg gacgaaaaag aagcgttgac caaagaaaga atccaagaag ctaaaaactc
     3781 aaataccgga ttagaaaaat tggtcaaaga aaatctacaa aattctacaa gtagtcaagg
     3841 tggccgatca gtagatgtcc gagaaaatat tcaaggttca ttgaatggtc atgtcggacg
     3901 gaatgttgat gtaagagaga atttacaaag ctcttcaaat ggtcaaggta ctaagacaat
     3961 tgatgttcga gaaaatgtac aaaattctac aagtagtcaa gggtttaaat caatggatgt
     4021 aagagagaat gtacaaaatt ctacaagtag tcaagggttt aaatcaatgg atgtaagaga
     4081 gaatgtacaa agttctacaa gtagtcaagg attcaaaaca gtagatgttc gggagaacgt
     4141 tcaaaaagat agtggagaaa tgaaagaaaa aacacaaaaa atcaatatcg ttgaagacag
     4201 aaagtcagcg aaaaaatttg attcaaatca tgaaacagtg attattgata gtgaaatccg
     4261 tgaaaatgat aagggatcaa aaccaagacg aagatttacc tatgaagaca acgagttatt
     4321 cagagataca cttaatgatc ctaatccgct tttcgataat ctattaaaaa aataaaaggg
     4381 ggaattgtca tgaaattaat tctaaagatt agtacgcatg atttcttaga acttgacgaa
     4441 ttatgtgaat cagtaaagaa cgcaacaaat gaaaaaatca cggtcactaa ttatattgaa
     4501 ctgatgattc aagaagaatt aaagatgaaa catgagataa aaaacaactt gaaatattgt
     4561 tatctagaaa aaagtgaaga gataatccat gaattaaatg gcttttaaaa attgtatttg
     4621 acatgaattt acgacaagga attagtgggg attaagcgat tattctcttc cactttagag
     4681 aaataatggg ttaaaaagcc agaatacgta gagcaatatt gacaacctat aaaggagcta
     4741 ttgttgaaaa ttgaagtaac aaaaagataa agagaggttt acatctatca gattaaaagt
     4801 gatagtacac ccatgaaacc tgcaaaagag ttgaacctaa tgtatatcgg tttaactctt
     4861 tttatttgca aattaaaaaa atatcaccga agtgacgaat gaaactatga ccaaattaag
     4921 caagaaaata tatcttgcaa ataaagtagt ataaaaagat attgataaag ccaatcaaaa
     4981 ctttgaattt caaacgttag ataatcaaac agatacaaaa aatgaaatca gttctacgcg
     5041 tacgattatt aaaaataata aattttcact agaagaaaat ggaaataaaa tcaaataatt
     5101 aggaagaaaa aacaaagcaa ttagaaaagc ctgcaagcaa atttgagtaa tcgagatcca
     5161 atttttttaa ggaagtttaa
//
LOCUS       DACCZY010000153         5156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCZY010000153 DACCZY010000000
VERSION     DACCZY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.160"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..207)
                     /locus_tag="IVZ67_002619"
     CDS             complement(<1..207)
                     /locus_tag="IVZ67_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8166641.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDE"
     gene            431..1231
                     /locus_tag="IVZ67_002620"
     CDS             431..1231
                     /locus_tag="IVZ67_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8166642.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1250..3118
                     /locus_tag="IVZ67_002621"
     CDS             1250..3118
                     /locus_tag="IVZ67_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8166643.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3111..3602
                     /locus_tag="IVZ67_002622"
     CDS             3111..3602
                     /locus_tag="IVZ67_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8166644.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3590..4144
                     /gene="srlA"
                     /locus_tag="IVZ67_002623"
     CDS             3590..4144
                     /gene="srlA"
                     /locus_tag="IVZ67_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8166645.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4162..>5156
                     /locus_tag="IVZ67_002624"
     CDS             4162..>5156
                     /locus_tag="IVZ67_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8166646.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1806 a          821 c         1061 g         1468 t
ORIGIN      
        1 ttcatcaact tttctagtaa tcattgggaa aaatttcaag cagtagatcg cacaaatacc
       61 tacgacgata taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac
      121 taaatcaagt tcgaacaagc ctaccaacag ccagttcaac cctcccacaa tcaataaagt
      181 taatgcaata gcatctaaag ttttcattag aattctcctt tcttaaatga taaaacaatt
      241 atatatcaaa aagagaaaca ttgcttatat aacgctcagg aattttagca atctatgcaa
      301 aaagatgaaa attttttttg ctcttccatt agtacacatt ttttagctga agctgtaaat
      361 ttcatgtctt aatttttttt agtgagcttt tatactggat ttgtaagaaa aaagaaggag
      421 aggatcacat atgtcggatt ggttggatat tgcaggaaaa gtagtaattg taactggagg
      481 ttcctcagga attggaagaa gtatcgtcga aaatttgttg aagcagaatg cacaagtagc
      541 gaattttgat gtaactgaat gccgtataca gcatgaaaac ctactatctt taaaagtcga
      601 tgtgtcttct aaaacggaca tagaagaagg gatatataaa gtaatgaaac atttcaagac
      661 gatcgatggt ctagtcaata atgcaggaat aaatattcct tctcttctaa tagatagaaa
      721 tcatccaaaa agcaagtatg aattatcaga acaagttttt gataagatga tcgctgtcaa
      781 tcaaaaaagt gtatatttga tgagccaagc agttggaaga atcttggtgc aaaagggatc
      841 aggagtgatc gtgaatcttt cttctgaatc aggattggaa ggatcagaag gacagagctg
      901 ttatgctgct acaaaagcag cgatgaacag ttttactcgt tcttgggcaa aagagttagg
      961 aaagaggaat gttcgtgtgg taggtgttgc accagggata ctggaagaaa ccggtttgag
     1021 aacacaagaa tatgaagaag ctctttctta tacgagaggt ataagcgtcg aacagctgag
     1081 aaacggttac agtcgaacaa gtacgatccc tctaggaagg agtggtaagc tgcaagaagt
     1141 ggcggatcta gtctgctatt atctttcaga aagatcaagt tatatcactg gagtcacgac
     1201 taatatttca ggaggtaaaa caagaggata aaggataggg gggacgcacg tgacgctagt
     1261 aaacagatgg taccaattat tgaaattact agtcactcac aagaaagttt caatccagga
     1321 agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa aaagatattg gggagttgaa
     1381 tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac aaacattttg aacttgaaat
     1441 ttatgatttt gatttttttg atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt
     1501 caattcggca tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa cagaggattt
     1561 cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc agaggaacag tggtcaagga
     1621 tctaaagaca ttaaaagaga tgatcaatga ttttgatgta aagattacag gcacaccaaa
     1681 taaaggacta agaataatag gagatgagct agaattacga ctacttttat tttattttgt
     1741 ttctccctac ttttcagaga cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa
     1801 acattttcag aagaagacga atgctagtaa acaagaaatc acccttttaa cgaaagttat
     1861 cggtatttct ttgaatagaa tctctagtaa ctatctgttg aagaagccca tcaatcgtta
     1921 cagtgggtgt tttgaatata tccaagaatt caatgaactg atcaatcagt tggaagaaat
     1981 ctatgaacta actttgagcc aatatgaaaa agattttatc agttttcctt tgaacatcat
     2041 caacacaagt ataacgatga taaggaaagc taactctgat ttgcgcttat atttcgaccc
     2101 catgatgatg cgtattcgac gattagatct cattgatctg gatgagtcct tcttatatca
     2161 agagatgaaa cagcacctct ctttattgat aaatcgggta atttttcgtt atcgattgac
     2221 tgatttattt tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga
     2281 atgcatacaa gcattagaag ctatcttgca acataagatc tctaaagtag agatcagcta
     2341 cctaacgttg tatttggagt tagcatttcg atatcaaaaa gagcattttt cctctaaaaa
     2401 agtagccatt gttcatacga caggaaaagg aacagcatta atgatccaaa gacaaatcaa
     2461 aaggatattg gggagggata tacaaataac acatgtttca gaagaacaat atgaaaaagt
     2521 agatctaaac aaatatttcg ctgtttttac aacgattccg ttaaaaaatg taaatgaaga
     2581 gacaccggtg atccatttga ctaatctttt tgatgactct tggttaaggg cacaatggca
     2641 aaaagcaaaa aaaaagcgat tagccgggtt tcattctact gtttttttct atcaaatatg
     2701 gaaagaaaaa aagacgtatc aagaatattt aaacaaatta gtcgctcctt tgatcagagg
     2761 ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac
     2821 atttgttaac aaagaatttg cttttcctca cacaaccaac cagctatctc aacacattgt
     2881 tttatcagta gggctaataa ctgatcaagg tttagcagaa acaggaggaa atggagttat
     2941 ttttttattt gccatacctg aagaaatgac ggctgaagtt gagaatgaat tatttgagct
     3001 atacgatatc ctatttgaac tcataaaaat gggaatagat gagtttattg aattagcacc
     3061 gacacaagaa tcatttttaa aactaatcaa aagtaaagga gagagacttc atgaatgata
     3121 tgtatctatt catagttttc gcagcttcag cttatgtgct gcaagcagtt atcagttgga
     3181 agcaactaaa gcatttcaat caaacatata gcgaactgcg aagaaaagga aaagtagcga
     3241 ttggtaaacg acctgggaaa atccgttcag gcacgattgt tatgctagct atagacgatc
     3301 atgatcaagt tttagaagtc agaaaaatgc aaggggttac atctttggca aaatttcatt
     3361 cattacctgt ctatgcgggt gaagatatcc attacatcga tcgttaccat ccctcagttc
     3421 gagcggaaga tcgtttgaca caagcagctt tagaaaatgc cagagaagta tacttacgaa
     3481 tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc ttttattgct atgctcaata
     3541 attttcggat atggaaaagt acgatcaaag aaaaaaggag tgacaaacga tggatatcat
     3601 aacaaaattt gctgagggat ttatgaggct gttccaaaca ggtgcagaaa cattcataag
     3661 ctggatgaca ggaatcgtac cagttgtctt gatgctgatg gttgcaatga atacgctgat
     3721 tgctttttta ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga aaaatccgct
     3781 tactcgttac cttgtgttac cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt
     3841 tacaatggct cgtttccttc cagaatacta caaaccaagc tattatgctg ctcaagctca
     3901 attttgtcat acgagcaatg gtgtattccc ccatattaat cctggagaat tgtttgtatg
     3961 gatggggatt gctcaaggga tcgaaacctt agggctaaat tcgatggagc tggctatccg
     4021 atacttgctt gtgggactgc tgatgaactt tattggtggt tggataactg attttactac
     4081 tggatttgta tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc tttcagttga
     4141 ttagagaaag gagagtttta aatgtatggt gatattaaga tcgtaagagg aaacggcggg
     4201 tatggaggtc ctttgattat taggccgaca aaagataaac acaagttcat ctatgtcaca
     4261 ggtggaggtg agaggccaat gatcgtagat aaaatagaag aactgacagg tatggagtct
     4321 atcaatgggt tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat cgttgactgt
     4381 ggagggactc ttagatgtgg aatttatcct aaaaaaggga taccaaccat caatatcgtt
     4441 tccacaggaa aaagtggccc attagcacaa tatatcactg aagatattta tgtttcagca
     4501 gtagggttag aacaaataca gccactaatg gaaactgatg ataacaaaga aacagtaaat
     4561 actgcatctc cagataaaaa gtacaaatat gatacttcta aaaagataac cgagcaaaaa
     4621 gcagagcagc aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca
     4681 ttcaatcaat cagcaagaga agcagtagat acgatgatcc ggacagtgat cccttttatg
     4741 gctttcgttt cccttttgat tggtgtgata caaggttctg ggatcggtaa tgtatttgct
     4801 aacattatgg cacctttagc aggaaatgca gttggattaa caatcattgg ctttatttgt
     4861 tcactgccat ttttatctcc tttgcttggt ccaggggcag ttattagcca agtgattgga
     4921 acattgatcg gtgtagagat tgggaaagga aatattcctc cccaattagc attaccggca
     4981 ctatttgcta tcaatactca aaatgcatgt gattttattc cagtcggtct aggtttggaa
     5041 gaagcaaaag ctgagacagt agaagtaggt gtaccatcag ttctttattc aagatttctt
     5101 aacggcatcc cacgagttat cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACCZY010000154         4798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCZY010000154 DACCZY010000000
VERSION     DACCZY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.162"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVZ67_002625"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IVZ67_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8166647.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IVZ67_002626"
     CDS             complement(2066..3064)
                     /locus_tag="IVZ67_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8166648.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IVZ67_002627"
     CDS             complement(3290..3943)
                     /locus_tag="IVZ67_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8166649.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVZ67_002628"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IVZ67_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8166650.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1407 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa ttttttta
//
LOCUS       DACCZY010000155         4740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCZY010000155 DACCZY010000000
VERSION     DACCZY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.163"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            153..959
                     /locus_tag="IVZ67_002629"
     CDS             153..959
                     /locus_tag="IVZ67_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8166651.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1804
                     /gene="vanW"
                     /locus_tag="IVZ67_002630"
     CDS             977..1804
                     /gene="vanW"
                     /locus_tag="IVZ67_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8166652.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1801..2772
                     /locus_tag="IVZ67_002631"
     CDS             1801..2772
                     /locus_tag="IVZ67_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8166653.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2765..3793
                     /gene="vanB"
                     /locus_tag="IVZ67_002632"
     CDS             2765..3793
                     /gene="vanB"
                     /locus_tag="IVZ67_002632"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8166654.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3799..4407
                     /locus_tag="IVZ67_002633"
     CDS             3799..4407
                     /locus_tag="IVZ67_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8166655.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1332 a         1002 c         1262 g         1144 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggtggctg gtacgccatg cggacaaaga taccccctat aaagacggcc ttacggtgac
     1321 caatggtaaa ctcaccacca tgtcgggcgg cggtatgtgc cagatgagca atttactatt
     1381 ttggatgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1441 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1501 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1561 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1621 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1681 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1741 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1801 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1861 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1921 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1981 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     2041 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     2101 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2161 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2221 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2281 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2341 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2401 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2461 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2521 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2581 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2641 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2701 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2761 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2821 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2881 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2941 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     3001 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     3061 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3121 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3181 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3241 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3301 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3361 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3421 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3481 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3541 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3601 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3661 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3721 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3781 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3841 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3901 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3961 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     4021 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     4081 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4141 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4201 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4261 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4321 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4381 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4441 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4501 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4561 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4621 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4681 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DACCZY010000156         4732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCZY010000156 DACCZY010000000
VERSION     DACCZY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.164"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..226)
                     /locus_tag="IVZ67_002634"
     CDS             complement(<1..226)
                     /locus_tag="IVZ67_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166656.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAE"
     gene            498..842
                     /locus_tag="IVZ67_002635"
     CDS             498..842
                     /locus_tag="IVZ67_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8166657.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            843..1262
                     /locus_tag="IVZ67_002636"
     CDS             843..1262
                     /locus_tag="IVZ67_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8166658.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1354..1704)
                     /locus_tag="IVZ67_002637"
     CDS             complement(1354..1704)
                     /locus_tag="IVZ67_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166659.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1670..3001)
                     /locus_tag="IVZ67_002638"
     CDS             complement(1670..3001)
                     /locus_tag="IVZ67_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8166660.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3257..3823
                     /locus_tag="IVZ67_002639"
     CDS             3257..3823
                     /locus_tag="IVZ67_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8166661.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3968..4055
                     /locus_tag="IVZ67_002640"
     tRNA            3968..4055
                     /locus_tag="IVZ67_002640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4000..4002,aa:Ser,seq:cga)
     gene            complement(4107..>4732)
                     /locus_tag="IVZ67_002641"
     CDS             complement(4107..>4732)
                     /locus_tag="IVZ67_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166662.1"
                     /translation="FLEAIRNDDDERYLFFSLLAFTGIRKGEAFALTWSDIDFKSKTL
                     NINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIVTIQRENLIF
                     HHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHASLLFESGASLKDVQERLG
                     HADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT         1663 a          808 c          896 g         1365 t
ORIGIN      
        1 cctcagcagg aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt
       61 caaagtattc aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt
      121 ggcgtccacg tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg
      181 cttctatcat tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc
      241 cttgtatgct ttagcgggta ttacaatttg agtgtaacat aaatggcttt tttatttgtc
      301 tcgcttaatt atacaaacgg cgtaaaataa aaaaagcgtt agcaactatg cttaaaaagc
      361 atatcgactg aataaactaa gcattggaca ggctgaaaga ctacatttaa aatgaaatgc
      421 gaagaaacaa tagaaaataa agttgcctta gaaccatagt tgaatcacct aacagtcgtg
      481 aagggagaat aagagaaatg gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt
      541 ttgcaccaaa atttgcagaa ttgaatgatg atgttttatt tggacaagta tggtccagag
      601 aagaattatc tgctcatcag cgtagtatca tcaccatatc tgcattaatt tcaggaggaa
      661 attttgaaca attgccagct catctgaaga ttggcaagca aaatggcatt actagtgaag
      721 aaatagcaga aatcattacc catttagctt tttatgtagg atggccaaaa gcttggtcag
      781 cttttagttt agcaaaagaa atctatttag aagaaaatag cgaagataag aaagaggcgt
      841 aaatgacgac aaaacacgaa gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa
      901 acgaagctta cgctcaagta tttaccgggc aaagctatct gaatatgtta gtgaatgatc
      961 ccgaagtttc tgttggtgta ggtaatgtaa tttttgaacc aggttgtcgc aatcactggc
     1021 atgtccataa agacggctat caaatcttac ttgtgaccgg aggagaaggc tggtatcagg
     1081 aagaaggaaa gccagcaaga cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga
     1141 ttaaacattg gcatggtgcg acaaaagaca gttggtttga acatttggcg atcactgctg
     1201 gtaattcaga gtttttagaa cctgtaacag atgaagtgta tgaaggattg gaaaatcaat
     1261 aatgtcaagc ttgaacagct cctgaaagta gattttaatc gaactttcag gagctgcttt
     1321 tttgattcag tcttttataa atagaaaata gggttaataa taggaatgag aagcacgtcg
     1381 aaagatctta atattgataa tcggctgggc atacatgatt tccacgtcat caatcaaact
     1441 acctaacaga tgataatctt cccgatcatg tcggggatta cctaatagtt cttgatagta
     1501 gatttccata ctagggtccc gaggattgtt cagcaaatca gcaagtacat ctaaatcttc
     1561 tggctgagtt tctgccgtac cctgtatctc tatgaaaagc ccttcctcag caaattctac
     1621 tccaatacgt atttcagtta tttcatggga atataaataa gtaagaagct catcgatgat
     1681 tctttttgat aattcttttt ccaagtgtct ttttttcctt tctgacagcg gtaaagttat
     1741 ctagtatcgg tacaaggata cctgcaacga aaccagttga aaaaccactg ttatatagat
     1801 ttagtcctcc gtgcaaagtg ctgacgttat ggacaagagc aatatgtagt acaccagcta
     1861 ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc gagacctgtc ccaaacacag
     1921 ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa gaagcaataa
     1981 atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca ctgaaaccca
     2041 ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc agcaaaacat
     2101 aagaagacag catcagacca actaagccta tgttgatcaa agtagcacca ataccagaat
     2161 tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga atccccaaaa
     2221 gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa
     2281 tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg attgaatagc
     2341 tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat
     2401 tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc ggaagaagta
     2461 gaccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa ggaaaacgca
     2521 taccaaaagt gatataacta acgaccggtg ataatgcgct accaaacagt ccgatcatga
     2581 tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct aaaatgattg
     2641 agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag
     2701 caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca gaaagtaagg
     2761 tcaaaatccc tacatttatg aatgcgcttc cgaaaccgcc taaagcaaaa taatctgtca
     2821 aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca gtaggagtat
     2881 tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa aaatactttc
     2941 ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt tcgttttcca
     3001 ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat ttggacaact
     3061 atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca aattttttta
     3121 attttgcaaa aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta
     3181 aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag
     3241 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
     3301 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
     3361 gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa
     3421 tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac
     3481 tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag
     3541 tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc
     3601 atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga
     3661 atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat
     3721 gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat
     3781 tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa
     3841 tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga
     3901 tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag
     3961 tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta
     4021 ataccggcgc gcaggttcaa atcctgtacc ttccttatat atcaacgatt agacacgatt
     4081 tcgatcgtgt cttttttgtt gtatatttag cctaaaatgt tttttgactc cccttttgac
     4141 tccccctgag tactgttctt cataaatgct acaaaattat tgatgacttt tttgttttga
     4201 gtttcagtta catgagtgta aatatttgaa gtagtttgaa tgtctgcatg ccctaaacgt
     4261 tcctgaacat cttttaaaga agctcctgat tcaaaaagga gagaggcatg agtatgtcga
     4321 aaaccatgag tagtgattct tttagtcaat tctggatgat gcttatatat gacattaagc
     4381 cacgaacgtg aaactgtagg gttatagaga agaccatcat ggtgaaaaat taagttttcc
     4441 ctttggattg taacgatcgt tttgtttttt gtataatact ttctcaataa actgatcaaa
     4501 tcattgtcca gataaatttt tctttttcca gattttgatt taggagtatt tacgatcaat
     4561 cttccttgat aacctcttgt tacagtttta ttgatattga gtgttttgct cttaaagtca
     4621 atatccgacc aggtcagagc aaaagcttct ccttttctta tacctgtaaa agccaatagg
     4681 gaaaagaata aatatctttc atcatcatcg tttcttattg cttctaaaaa tt
//
LOCUS       DACCZY010000157         4668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCZY010000157 DACCZY010000000
VERSION     DACCZY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.165"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..191)
                     /locus_tag="IVZ67_002642"
     CDS             complement(<1..191)
                     /locus_tag="IVZ67_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166663.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="IVZ67_002643"
     CDS             complement(201..1016)
                     /locus_tag="IVZ67_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8166664.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="IVZ67_002644"
     CDS             complement(1278..1721)
                     /locus_tag="IVZ67_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8166665.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1855..2193)
                     /locus_tag="IVZ67_002645"
     CDS             complement(1855..2193)
                     /locus_tag="IVZ67_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8166666.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="IVZ67_002646"
     CDS             complement(2181..2558)
                     /locus_tag="IVZ67_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8166667.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="IVZ67_002647"
     CDS             2782..3975
                     /locus_tag="IVZ67_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166668.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="IVZ67_002648"
     CDS             complement(4141..4563)
                     /locus_tag="IVZ67_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8166669.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1365 a          891 c          820 g         1592 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattattt
//
LOCUS       DACCZY010000158         4667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCZY010000158 DACCZY010000000
VERSION     DACCZY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.166"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            95..616
                     /locus_tag="IVZ67_002649"
     CDS             95..616
                     /locus_tag="IVZ67_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8166670.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(1315..1872)
                     /locus_tag="IVZ67_002650"
     CDS             complement(1315..1872)
                     /locus_tag="IVZ67_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166671.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(2051..2662)
                     /locus_tag="IVZ67_002651"
     CDS             complement(2051..2662)
                     /locus_tag="IVZ67_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166672.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2775..3032)
                     /locus_tag="IVZ67_002652"
     CDS             complement(2775..3032)
                     /locus_tag="IVZ67_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166673.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(3629..3907)
                     /locus_tag="IVZ67_002653"
     CDS             complement(3629..3907)
                     /locus_tag="IVZ67_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166674.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3941..4192)
                     /locus_tag="IVZ67_002654"
     CDS             complement(3941..4192)
                     /locus_tag="IVZ67_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166675.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1467 a          935 c          667 g         1598 t
ORIGIN      
        1 ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt tttgtcagac
       61 atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga ttgatgcaac
      121 gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta aaaaaatggt
      181 cgaattcatc aacttagctt gtccgtattg ccgacaatgg tttgaagaat cttatgagct
      241 attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt tcgataaaga
      301 aaaagaaagc ttacagcgag ggaacgtgat gcaccggtat ctcacaatca gcgatggcca
      361 aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga aacatctttc
      421 tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac aaaaagacga
      481 acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat ttgttccaac
      541 tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat taaaagaatt
      601 gatccaaaca aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca cttttgtctc
      661 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcttatt tcttggagtt
      721 aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc
      781 cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat aatttattca
      841 tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaatttc
      901 ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca ctttttaagt
      961 tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta
     1021 gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc
     1081 tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac
     1141 tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg ctctcttttt
     1201 tattcagatt catttttgct gatcacagcc tagtatttca atggttttga acactaaacc
     1261 aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt tcgattattc
     1321 aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc caaacctatc
     1381 gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt tattgcgtgg
     1441 atattctatt gttacgccat taaatggata ccaattagga ttaggctctc tttttttctg
     1501 tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc gaccatcctc
     1561 taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta gacgccacgt
     1621 gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg caacaattgt
     1681 atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat caaactcccc
     1741 tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta aaaccgcatt
     1801 cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt ttctatttga
     1861 taaatttctc attattcgca catcacctct acattctcac atattagttc gtaatactcc
     1921 tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct actactcgta
     1981 taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt ctatttatcg
     2041 taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc cagttgatta
     2101 aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt acagttctta
     2161 tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc tcgagatttc
     2221 taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata taatattgcg
     2281 cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct tctctaccac
     2341 tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg aactcacacc
     2401 cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct agttcgtatt
     2461 caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta ggtgagatta
     2521 atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct atgatcacaa
     2581 ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg tattccattt
     2641 tattccccct attactattc atatccgcct atctatatcg atcttccaat gcagagataa
     2701 ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt ctccactaag
     2761 gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac tcctccaaaa
     2821 acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt atatgtgttt
     2881 gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat cgcattcgta
     2941 tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat cattgtgatt
     3001 tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta tagaaatcgc
     3061 ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg tctccatttt
     3121 tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca caacacgtta
     3181 ccttttaaat cattatactg tatctcatac attattatca ataaactatt ataaaaatgc
     3241 aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa tcataccctg
     3301 tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg caagacagac
     3361 actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt acccactttt
     3421 gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac cccaagaaat
     3481 taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca acatttaaat
     3541 tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata tcaagatgtc
     3601 aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta ttaagactaa
     3661 tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat gatcctgcaa
     3721 gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc tttttcccat
     3781 taggatcttt tattttatga attttctgca ggtaatctct cctaatggca gctattttta
     3841 cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt atgttgtgtt
     3901 tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa agttgaatga
     3961 gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca tatcctcccg
     4021 caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa gagaaaagag
     4081 tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag ataacaactc
     4141 caaacattga atttttatac aatgaccatt tatttatagg cttttctttc attttacttc
     4201 cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc tctttgaaca
     4261 tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt tataggcgag
     4321 ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact gaaattaaag
     4381 cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac atgtctttac
     4441 ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg taatactttt
     4501 tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata atatctaatc
     4561 taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa ataaatagaa
     4621 actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgtat
//
LOCUS       DACCZY010000159         4593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCZY010000159 DACCZY010000000
VERSION     DACCZY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.167"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            189..2858
                     /locus_tag="IVZ67_002655"
     CDS             189..2858
                     /locus_tag="IVZ67_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8166676.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="IVZ67_002656"
     CDS             complement(2949..3569)
                     /locus_tag="IVZ67_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8166677.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="IVZ67_002657"
     CDS             3683..4222
                     /locus_tag="IVZ67_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166678.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="IVZ67_002658"
     CDS             4219..4557
                     /locus_tag="IVZ67_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP8166679.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac att
//
LOCUS       DACCZY010000160         4561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCZY010000160 DACCZY010000000
VERSION     DACCZY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.168"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            186..1295
                     /locus_tag="IVZ67_002659"
     CDS             186..1295
                     /locus_tag="IVZ67_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8166680.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1378..2274
                     /locus_tag="IVZ67_002660"
                     /pseudo
     CDS             1378..2274
                     /locus_tag="IVZ67_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2290..3114
                     /locus_tag="IVZ67_002661"
     CDS             2290..3114
                     /locus_tag="IVZ67_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8166681.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3146..4456
                     /locus_tag="IVZ67_002662"
     CDS             3146..4456
                     /locus_tag="IVZ67_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8166682.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
BASE COUNT         1530 a          688 c          907 g         1436 t
ORIGIN      
        1 aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag
       61 ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta
      121 aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa
      181 gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa
      241 cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag
      301 atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg
      361 gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg
      421 ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg
      481 ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat
      541 ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct
      601 atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg
      661 ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag
      721 aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag
      781 aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg
      841 aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt
      901 ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg
      961 ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg
     1021 aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg
     1081 aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg
     1141 agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc
     1201 aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg
     1261 aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg
     1321 cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg
     1381 aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt
     1441 tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt
     1501 ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac
     1561 caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca
     1621 gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga
     1681 aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca
     1741 tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact gattaataac
     1801 atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt
     1861 tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta
     1921 gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt
     1981 ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta
     2041 gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact
     2101 cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca
     2161 tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata
     2221 ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc
     2281 tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta
     2341 ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg
     2401 gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat
     2461 caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt
     2521 gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt
     2581 ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc
     2641 ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca
     2701 attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa
     2761 tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt
     2821 tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga
     2881 atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca
     2941 attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac
     3001 ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc
     3061 gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat
     3121 taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac
     3181 tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca
     3241 tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa
     3301 tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga
     3361 agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc
     3421 aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac
     3481 catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc
     3541 taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc
     3601 gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag
     3661 cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt
     3721 aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac
     3781 aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc
     3841 acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc
     3901 aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat
     3961 tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga
     4021 ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg
     4081 tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg
     4141 aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc
     4201 ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt
     4261 agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac
     4321 actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt
     4381 gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt
     4441 acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt
     4501 cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta
     4561 a
//
LOCUS       DACCZY010000161         4522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCZY010000161 DACCZY010000000
VERSION     DACCZY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.169"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..641
                     /locus_tag="IVZ67_002663"
     CDS             <1..641
                     /locus_tag="IVZ67_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8166683.1"
                     /translation="YVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKL
                     AQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLS
                     PLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTA
                     KIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            658..1092
                     /locus_tag="IVZ67_002664"
     CDS             658..1092
                     /locus_tag="IVZ67_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166684.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1709..>4522
                     /gene="esp"
                     /locus_tag="IVZ67_002665"
     CDS             1709..>4522
                     /gene="esp"
                     /locus_tag="IVZ67_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8166685.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHK"
BASE COUNT         1669 a          648 c          881 g         1324 t
ORIGIN      
        1 aatatgttaa aaatggagat attcgaaatt tagaaaacat ggtttttaat ctgagtaatg
       61 gaataattcc aagtgttagt ggagatacga ttcggtctga aaaaaattat tctatcattg
      121 tttttgaaaa gttagcacaa acgtctataa cattaggaat ggatataata gaagcgtatc
      181 aaagtagaga tgctttgata caagaaaacg aattagctgt atcgttacct gaggtcttaa
      241 aagtgaggga ttctgggatt gtttattata ctaaggaaat cggaaaaact aagatagagc
      301 atctttctcc cttaatttct tctgtcgttc aattcatcgg cttaaatatc tataaaagaa
      361 ttactgtaaa ggaaatagct aattattttt ctgtaagtga aactaaatta agagaaagct
      421 ttaagaatga aatgcatatt acgatttata attatatctc taagcgaaaa atttctacgg
      481 ctaaaataat gttgaaatca aaccatacta tcagtgaagt ttctttaggt ttaggatttt
      541 cagattcttc acacttttcg agagtattca aaaagtacgc aggtgtatct cctaagcaat
      601 atcaattagg tttagtggac aatttcaaca acattagctg agaggtttta ttttattatg
      661 agactatatc atttacgtag tttggaggag ttgaggaaat tggattttaa caaaaaattt
      721 ttttgggaaa tttctgattt gagttgtttc gtagaatctg agcgtctaag taatggtaat
      781 aatagtgtgt taaatgaata tgacaaagta gtaattaata aagtaaaaag tttatgtgaa
      841 gaagctagag aatctatcag tcatattaat ggtagcaatt ttaaacaaac attatttaat
      901 ctgattaagc tagatgctaa aatatcaagt tatttattct ttttgataca tgatgaattt
      961 atggattatc aaaaattgga tcagttaatt gaaagagaaa gctgggaaga ttattacgtg
     1021 ggtctaactt tttcagaaaa gttaaacaac tacaagttaa tagattataa gtatttgagt
     1081 gaaagcaatt aattattcat gtagagtact agacacttta aatatggaca attcaattat
     1141 aaagaaaaag aatcaataat ttaatgaggc tgtgacaaat gtcatttttt gtttcgctaa
     1201 aaacgagtga aaaaatgttg ataactccaa tcattcgttt aagattctag gaatcattca
     1261 gaaatgctgt gaaaccgata ctttataatt ataaagaagg tgatttcaaa ttccgaagaa
     1321 ggaatttgaa gtcacctttt tacttatgtc acagtctcat tcagtaatgc gggataaggc
     1381 tccttattcc gttatttgaa tactctgaag tttaatctaa gaaagggtaa tattttgaaa
     1441 acatgcttca tcgcatagaa atgaacttaa tttcagaaag aatacataca tatcttttta
     1501 tatgttttca gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat
     1561 gtactttttt tgttgtaatg ttcatgaaat tctctaagta aattagttaa cataaggtta
     1621 gtaagattcc tattctgtga ataatacgta tcagatttat cttttattct tactaggaaa
     1681 gtaattatta agggagttga tttgaataat ggttagcaag aataataaga gagtatttct
     1741 agaaaaaaca aagaaacgag tattgaagta cagtatcaaa aaattaagtg ttggggttgc
     1801 atctgttttg attggtgttg gtcttgtctt gggaacaact gaactagtta aagcacaaga
     1861 tgaaatttct ccaagcagcc cacttgaaac agcgataagc tccgtacaag taggtgacaa
     1921 agtagcttcg gggaatactt ttcaggagaa tcctggttat acgaaaaatt ataacttttc
     1981 ggatttacaa tttagtcctc aagaactaac tggagacaca ctaaaaggca atacaattgg
     2041 atttgaagtt tacggaaaac ataatattgc agcatcaact aaaaactggg aaatccgtct
     2101 tcaattagat gaacgattag ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg
     2161 aattggatct tctagacgga cctttgtaag aattaatgat tcgctgggta gacctacaaa
     2221 tatttggaag gtcaattata ttcgtgcaag tgatggatta tttgctgggg cagaaacaac
     2281 cgatacgcaa accgctccta atggggtaat tacgtttgaa aagagcctag atgaaatttt
     2341 taaagagata ggcatagaca atctcaagac cgatcgttta atgtatcgaa tctacttggt
     2401 aagtcatcaa gacgatgata aaattgtacc gggtattgat agtacgggct actttttaac
     2461 agattcagat gatttctata atagcttaga tgtatctgaa aataatcctg accagtttaa
     2521 acatggatca gtaagtgcaa aatatgagaa acctaatact caaacaaaag atggttcagg
     2581 atccacaggt gctaatggag ctataatctt agatcataag ttaacgaaaa attataattt
     2641 ctcatattca tcttccgcaa agggcacgcc ttggtatgct aattacaaaa ttgatgaacg
     2701 attagttcca tatgtagcgg gtatacagat gcacatggtt caggctgaca aagtaacata
     2761 tgatgtttcg tttgaatccg gaacaaaagt agctgattta gcaatcgagc gacgggaagg
     2821 tcatgaaaat tatggcatag gttcaataac taataatgat ttaactaaac ttatcgattt
     2881 tgctaatgca agtccacgtc cagtcgttat tcgatatgtt ttacaattga ctaagccatt
     2941 agatgaaatc ttagaagata tgaaagcaac agcacaagtt gaagaaaata aaccatttgg
     3001 tgaagatttc atctttgatt cttggttgtc ggatacgaat aaaaaattaa tccagaacac
     3061 ttatggaaca ggttattatt atttgcaaga tattgatggt gatggaaacc ctgacgataa
     3121 agaagagagc ggagacacga atccatatat cgggaaacct gaattagaag aagtatatga
     3181 tgttgacaca acagttaagg ggaaagtatt catccacgag ttagcgggaa caggtcacaa
     3241 agcccaactt gttgataaag aaggtactgt attagcagaa aaaactatcg ctccaaatga
     3301 aaaagatggg gctccaattt cagatactgt agaatttgaa tttacgggtg tagattcaag
     3361 taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc
     3421 agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg gttgataaac aaaccgtggt
     3481 agttggattt aaaccagatg ctaaagaatc aattcggaat aataaaaact tacctgaaga
     3541 tgcagagtat tcatggaaaa cagagcctga tacttctaac gttactgata gtacgaaagg
     3601 tattgtaact gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa
     3661 agcttctcaa gctatggaaa atgatgcaac atacgtacct ataacaacaa ctccagaaac
     3721 gacagttcaa agtggaaaac ctacatttga taaaccagat gttcctttag ctaaagatgc
     3781 cttttcaatt tcagatgtct ataataagta cttcggtaat gcaagtgttg atgcaaacac
     3841 tggtattgtt acattcactc cagctaaagg tgtaggagaa tcggagccga ttactggaac
     3901 aattcctatt aaaattgttt accaagatgg ttctgtaggc acgaccgatt tagcagtaac
     3961 tgtaagtaaa gatatttatg aaaatccagg agaaaacatt cctgcaggct accacaaagt
     4021 aaccttcacc gcaggggaag gaacaagtat tgaaagtgga acaacagtct ttgcagtgaa
     4081 agacggcgta agcttgccag aagataaact tccagtgttg aaagcaaaag atggttatac
     4141 agatgcgaaa tggccagaag aagcaactca accaattaca gcagatgata cagaatttgt
     4201 atcaagtgca acaaaactgg atgatatcat tgaaaatcca ggagaaaaca ttcctgcagg
     4261 ctaccacaaa gtaaccttca ccgcaggaga aggaacaagt attgaaagtg gaacaacagt
     4321 ctttgcagtg aaagacggcg taagcttgcc agaagataaa cttccagtgt tgaaagcaaa
     4381 agatggttat acagatgcga aatggccaga agaagcaact caaccaatta cagcagatga
     4441 tacagaattt gtatcaagtg caacaaaatt ggatgatatc attgaaaacc caggggataa
     4501 cattccggct ggctatcata aa
//
LOCUS       DACCZY010000162         4418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCZY010000162 DACCZY010000000
VERSION     DACCZY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.170"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            59..913
                     /locus_tag="IVZ67_002666"
     CDS             59..913
                     /locus_tag="IVZ67_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8166686.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IVZ67_002667"
     CDS             1010..3259
                     /locus_tag="IVZ67_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8166687.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="IVZ67_002668"
     CDS             3262..>4418
                     /locus_tag="IVZ67_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166688.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DACCZY010000163         4376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCZY010000163 DACCZY010000000
VERSION     DACCZY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.171"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            190..1038
                     /locus_tag="IVZ67_002669"
     CDS             190..1038
                     /locus_tag="IVZ67_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8166689.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1183..2124
                     /locus_tag="IVZ67_002670"
     CDS             1183..2124
                     /locus_tag="IVZ67_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8166690.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2114..4285
                     /locus_tag="IVZ67_002671"
                     /pseudo
     CDS             2114..4285
                     /locus_tag="IVZ67_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1367 a          855 c          818 g         1336 t
ORIGIN      
        1 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
       61 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
      121 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
      181 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
      241 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
      301 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
      361 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
      421 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
      481 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
      541 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
      601 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
      661 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
      721 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
      781 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
      841 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
      901 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
      961 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     1021 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     1081 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     1141 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     1201 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     1261 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     1321 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     1381 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     1441 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     1501 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     1561 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     1621 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     1681 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     1741 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     1801 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     1861 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     1921 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     1981 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     2041 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     2101 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     2161 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     2221 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     2281 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     2341 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     2401 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     2461 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     2521 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     2581 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     2641 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     2701 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     2761 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     2821 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     2881 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     2941 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     3001 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     3061 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     3121 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     3181 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     3241 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     3301 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     3361 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     3421 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     3481 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     3541 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     3601 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     3661 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     3721 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     3781 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     3841 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     3901 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     3961 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     4021 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     4081 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     4141 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     4201 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     4261 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     4321 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg tttttt
//
LOCUS       DACCZY010000164         4367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCZY010000164 DACCZY010000000
VERSION     DACCZY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.172"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            56..1357
                     /locus_tag="IVZ67_002672"
     CDS             56..1357
                     /locus_tag="IVZ67_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166691.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="IVZ67_002673"
     CDS             1384..1644
                     /locus_tag="IVZ67_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8166692.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="IVZ67_002674"
     CDS             complement(2643..3878)
                     /locus_tag="IVZ67_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8166693.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1275 a          695 c          894 g         1503 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1921 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4081 tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaaat
     4141 aaaataatta tattaattga taggtgtgct gtcgtcatta tacacacgta agaaagaaat
     4201 agttatgttt aaaaatattc ttagactttc tcgccatgac aattttcact atgtgttata
     4261 actgtaaacg ataattattc agataacaca aaaagaaatc aaggagcaga tacgccagtt
     4321 gtttaatttg aatattattt taaaattaaa atttttattc tgttcta
//
LOCUS       DACCZY010000165         4360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCZY010000165 DACCZY010000000
VERSION     DACCZY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.173"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1081)
                     /locus_tag="IVZ67_002675"
     CDS             complement(<1..1081)
                     /locus_tag="IVZ67_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8166694.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKK"
     gene            complement(1189..3216)
                     /locus_tag="IVZ67_002676"
     CDS             complement(1189..3216)
                     /locus_tag="IVZ67_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8166695.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3361..4092)
                     /locus_tag="IVZ67_002677"
     CDS             complement(3361..4092)
                     /locus_tag="IVZ67_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8166696.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1217 a          907 c          714 g         1522 t
ORIGIN      
        1 ctttttttct caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag
       61 ctactaagat ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt
      121 ccttggctac ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt
      181 tgatcgtagt atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa
      241 cagtcattgt aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa
      301 aagaaaaatt tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt
      361 cagaatgtcc tttttttgta atttttgttt caataactac ttgattgata taggtttttt
      421 gatcattttg aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt
      481 catgaagagt aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa
      541 tataggtgta ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt
      601 ccgtctggat ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg
      661 gcatcctgac atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg
      721 gatagctcac ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt
      781 ttccgtattc aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt
      841 tcacttcttt ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat
      901 caagttgaag atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg
      961 tcactgaaaa attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa
     1021 taactaggag tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca
     1081 tatgtaagag aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc
     1141 tgtgcccttc ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg
     1201 gacatctgca agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac
     1261 aggaatattt attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct
     1321 ttcacccttt tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt
     1381 ttcagaggta acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt
     1441 ctcttgtttc aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc
     1501 acctgaaaac tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt
     1561 gacatattga ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc
     1621 ctctgtattt tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc
     1681 ttcaatatca ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat
     1741 ttcagcaata tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt
     1801 cagctttgcg atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag
     1861 gtattcaaaa tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa
     1921 ttcgattatt aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt
     1981 tccggttatc aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg
     2041 aatagttaat tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc
     2101 cccctcgacc ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat
     2161 ggccccataa tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag
     2221 tcgcgtgaca gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca
     2281 atttacttct gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc
     2341 gacaccttcc aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa
     2401 gcgccatgtg ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga
     2461 ataattatta ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc
     2521 agcagttatc tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc
     2581 aatcgagagc gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc
     2641 catacctggc gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa
     2701 tgtaaaggtc atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt
     2761 aaatgtaaaa tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga
     2821 cctttctgct gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc
     2881 gataggtgtt tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac
     2941 cgcagaaatt ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc
     3001 agtggtctct ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga
     3061 ttctattaaa ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg
     3121 ctccaactga ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag
     3181 actgcttaaa atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta
     3241 aattcctaga tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc
     3301 tcgattacct cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa
     3361 ctagttgtta ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa
     3421 cttgacagat ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc
     3481 accaaatcga tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt
     3541 attgtttcct cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg
     3601 atcaaccaaa gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc
     3661 cccactggca gttttaaact gttctggttg agagacactt aatttccacc cagataaggt
     3721 acttctccta tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc
     3781 agcagctgca taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt
     3841 tgatccataa tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc
     3901 gacaggttta ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc
     3961 tgcttcaaat gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga
     4021 aacaaaagtc gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc
     4081 ttttttcatc attgacattc ctctttcttt atttactaaa catatcagtc gattggatta
     4141 atccatagca aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt
     4201 ggtcctatgt atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat
     4261 tgcaacttga tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt
     4321 aagtccccct cctatctatc aaaatgtgac ttgactgcct
//
LOCUS       DACCZY010000166         4309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCZY010000166 DACCZY010000000
VERSION     DACCZY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.174"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..923)
                     /locus_tag="IVZ67_002678"
     CDS             complement(27..923)
                     /locus_tag="IVZ67_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8166697.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="IVZ67_002679"
     CDS             complement(916..1596)
                     /locus_tag="IVZ67_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166698.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="IVZ67_002680"
     CDS             complement(1597..2379)
                     /locus_tag="IVZ67_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8166699.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="IVZ67_002681"
     CDS             complement(2372..3289)
                     /locus_tag="IVZ67_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8166700.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3776)
                     /locus_tag="IVZ67_002682"
     CDS             complement(3453..3776)
                     /locus_tag="IVZ67_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166701.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1262 a          944 c          755 g         1348 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagt
//
LOCUS       DACCZY010000167         4146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCZY010000167 DACCZY010000000
VERSION     DACCZY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.175"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(592..882)
                     /locus_tag="IVZ67_002683"
     CDS             complement(592..882)
                     /locus_tag="IVZ67_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166702.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1224..1520
                     /locus_tag="IVZ67_002684"
     CDS             1224..1520
                     /locus_tag="IVZ67_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166703.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1527..3479
                     /locus_tag="IVZ67_002685"
     CDS             1527..3479
                     /locus_tag="IVZ67_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8166704.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3551..4048)
                     /gene="dfrG"
                     /locus_tag="IVZ67_002686"
     CDS             complement(3551..4048)
                     /gene="dfrG"
                     /locus_tag="IVZ67_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8166705.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1409 a          732 c          711 g         1294 t
ORIGIN      
        1 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
       61 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      121 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      181 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      241 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      301 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      361 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      421 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      481 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      541 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      601 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      661 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      721 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      781 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      841 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      901 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
      961 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1021 aaaaaaaaga ggcagcagta tagatttaag tagttcagtt ttggagtaca aaaagtgaaa
     1081 atgtaaacct aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa
     1141 atcaacgttt gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg
     1201 aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta
     1261 agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt
     1321 ctgtggattg ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg
     1381 cagagcgtac tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga
     1441 agtttattga atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta
     1501 tcaaagatag agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag
     1561 atgaaattgt agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac
     1621 aatatgattt attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc
     1681 caacgtcttt taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc
     1741 cgttttgttg taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa
     1801 gtcgatataa aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg
     1861 acaatcctat tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt
     1921 ctgttgttga agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag
     1981 attatcaatt tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga
     2041 agcaatttta caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt
     2101 gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa
     2161 agaacaccat tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta
     2221 cttgtgatat tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata
     2281 ggcgttgttt ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta
     2341 acgagatgtg gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag
     2401 gacaaggttc aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg
     2461 tatctgctga ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata
     2521 tttcaatgga tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt
     2581 ttagtcgtaa aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg
     2641 atagcgaaaa taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat
     2701 ttgtcgatgg tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta
     2761 aacaaatggt taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa
     2821 acgcctttta tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt
     2881 gccaagttaa caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag
     2941 cagaactgct cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg
     3001 catctcatta tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta
     3061 aagatggaga aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta
     3121 aggataaagg attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg
     3181 aaatcgcaaa gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga
     3241 taagacgtta tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga
     3301 gctatattta cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa
     3361 cttactacaa cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc
     3421 aaagattagg gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac
     3481 aaaaagagct tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt
     3541 aacgcacccg ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg
     3601 gatttttatc attctttatc cctttttggg caaatacctc attccattcc tcataattca
     3661 cttctggaaa aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct
     3721 caacataagg gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt
     3781 cgtttttaca taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg
     3841 taaacccctt atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg
     3901 attcaaggtt cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt
     3961 cccagtcctt gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat
     4021 ccatcgcagc aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat
     4081 ctaatttctt atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag
     4141 caattt
//
LOCUS       DACCZY010000168         4114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCZY010000168 DACCZY010000000
VERSION     DACCZY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.176"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(153..1658)
                     /locus_tag="IVZ67_002687"
     CDS             complement(153..1658)
                     /locus_tag="IVZ67_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8166706.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVFRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="IVZ67_002688"
     CDS             complement(1655..2341)
                     /locus_tag="IVZ67_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166707.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="IVZ67_002689"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="IVZ67_002689"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8166708.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1134 a          866 c          703 g         1411 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgaa taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa agggatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attttggctc tataatttat gggactgatg aatcagaatt
     4081 gattcttcag ccccattttt attttaggta ttaa
//
LOCUS       DACCZY010000169         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCZY010000169 DACCZY010000000
VERSION     DACCZY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.177"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(121..579)
                     /locus_tag="IVZ67_002690"
     CDS             complement(121..579)
                     /locus_tag="IVZ67_002690"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8166709.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(750..1895)
                     /locus_tag="IVZ67_002691"
     CDS             complement(750..1895)
                     /locus_tag="IVZ67_002691"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8166710.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1908..2825)
                     /locus_tag="IVZ67_002692"
     CDS             complement(1908..2825)
                     /locus_tag="IVZ67_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8166711.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3253..3816
                     /gene="efp"
                     /locus_tag="IVZ67_002693"
     CDS             3253..3816
                     /gene="efp"
                     /locus_tag="IVZ67_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8166712.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1278 a          763 c          775 g         1257 t
ORIGIN      
        1 gctaaactga aaactagcta tttgttttcc cgtttagaac tcttttcaga caataaatat
       61 ttaataaaat acgaagataa gatagctata ttgcttctca tctttacgtt cttttagaaa
      121 ctatgcttct acttcagacc attcatcgcg ttttgctaaa aattcacgag ccaaagcttg
      181 tgcaccttct agtgtatggc tggcagccca gccacattgt gtttcattgc ttgcaggtac
      241 ttcagtagct tcaagaacat ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta
      301 gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg catcccattg gagaccagtc
      361 tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag
      421 acttggcatg tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt atttaatgat
      481 gacatcgcca tgcgcacccg tttttcgatc agctacacgt acataaggtg ctttgacttt
      541 tgtatggtca agattaaaac tttcgacatt cattctcatt tgtaagactc ctatctgttt
      601 gttcattcgt tttgatgctt ttcactaaag aattatttag tgaaatttac aaaaacgtct
      661 ttctacttga ttctaacact tatcgtttcc aaggtgtagc aatctgaaat attttttaga
      721 tttttctgct aagtcgttta ttgggacttc tagttcttga ttctgtcaaa tgcctgagct
      781 aaatcgttta tgagatcctg accatactca attcctacag aaagacggat caagccgtcc
      841 tttatgccag cctcttctcg tttttctttt ggaatagacg catgagtcat gacagaagga
      901 atttccacta aactttctac gccacctaaa ctttctgcta aagtaaatag ttggagtgat
      961 tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa aagaaatcat tccgctaaag
     1021 ccgctcatct gctgcttagc aactccatgg agttcatgat cgggtaatcc tggatagtag
     1081 accttttcta ctgctggatg agaaaataga aaatcagcta ccacaaaagc atttttttga
     1141 tgttcctcca tacgaacact tagtgtcttg attccgcgct gaagtaacca gctatcttgg
     1201 ggaccaagta cggctccgat agcattttgg taaaatccga tctgatcggc tagatcttta
     1261 tggttagaag tcactaaacc agctactaca tcgctgtgtc cgcctagata tttggtaccg
     1321 ctatggacaa cgatatcaga accgagatct aaaggtcttt gaaaataagg agtagcaaat
     1381 gtattgtcag caataactat caatccatgt tgatgagcca gagtggcact tttttctaaa
     1441 tcagtaatct ttagaagcgg attgctaggt gtttcaagat aaagtgcttt tgtattgggt
     1501 ttgatggatt gttctatttt atcaagatta ctcgtatcta tgatcgtgta ctcgagacca
     1561 tttttagtaa gaactttgtc aaatagacgg aatgttcctc catagacatc atctcctaat
     1621 aagatatgat cgccagcttg aaaaagagaa aaaacggcat gaatcccaga aagaccagaa
     1681 gaaaaagcga atcctcgaac accgccttcc aaatcagcaa tcagttcttc caatgcaaaa
     1741 cgagtaggat taccagatct tgaatattca tattgtttag gctggccgac gccatcttgc
     1801 cgataagtgg atgtttgata aataggtaca ctgacagctc ctgttgtggg atcttcactg
     1861 atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc tttcctccta taagtaaatg
     1921 ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt
     1981 ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga acttccaact
     2041 aatagtcctt gttctcgtgc taactgccta gtatagttaa acccttctac atcagagatt
     2101 gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc tacaccgata
     2161 ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc tggttctaca
     2221 ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc agcaaaggtt
     2281 ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc atgaaagatt
     2341 tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa ttgaagaggc
     2401 agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc accgatgatt
     2461 ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt
     2521 tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt gagtgcggcc
     2581 agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt tttttcattg
     2641 atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc tttgatactt
     2701 cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg catagaatag
     2761 tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat tgtcgagata
     2821 atcatgtggg cctcctaaaa aaagataata aaaaaaggag ctgactatta aatcagctcc
     2881 ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt actcattcta
     2941 tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca tacaacagca
     3001 acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta aaatttttat
     3061 taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat atttcgatag
     3121 catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa agatagagaa
     3181 tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata aagaaaagaa
     3241 ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt cgtacaagac
     3301 ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg aaatacagtt
     3361 atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acgacacaac gttcagacca
     3421 gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt gtacacgatg
     3481 gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat tcctgtagaa
     3541 acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat ccaatttttc
     3601 ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt tgtggaaaca
     3661 caaccttcca tcaaaggtgc aacagtcact ggttcaggta agccagcgac aatggaaact
     3721 ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga aatcaataca
     3781 gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt gataagaatt
     3841 tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc aagaggtttt
     3901 ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata ttttgaaaga
     3961 tgaatggcaa aagctagaga aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat
     4021 tcataaaatt gaaaatatcc gaactattcc gaactatatt gaagcgaaaa tat
//
LOCUS       DACCZY010000170         4067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCZY010000170 DACCZY010000000
VERSION     DACCZY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.178"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            86..1435
                     /locus_tag="IVZ67_002694"
     CDS             86..1435
                     /locus_tag="IVZ67_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8166713.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IVZ67_002695"
     CDS             complement(1563..2153)
                     /locus_tag="IVZ67_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8166714.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IVZ67_002696"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IVZ67_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8166715.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DACCZY010000171         3977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCZY010000171 DACCZY010000000
VERSION     DACCZY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.179"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..470
                     /locus_tag="IVZ67_002697"
     CDS             <1..470
                     /locus_tag="IVZ67_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166716.1"
                     /translation="IICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIF
                     SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLS
                     LIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(596..1273)
                     /gene="nth"
                     /locus_tag="IVZ67_002698"
     CDS             complement(596..1273)
                     /gene="nth"
                     /locus_tag="IVZ67_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8166717.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1280..1978)
                     /locus_tag="IVZ67_002699"
     CDS             complement(1280..1978)
                     /locus_tag="IVZ67_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8166718.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2246..3430
                     /locus_tag="IVZ67_002700"
     CDS             2246..3430
                     /locus_tag="IVZ67_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8166719.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3436..3879)
                     /locus_tag="IVZ67_002701"
     CDS             complement(3436..3879)
                     /locus_tag="IVZ67_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8166720.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1043 a          781 c          646 g         1507 t
ORIGIN      
        1 tcataatttg tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata
       61 aaaaagctga aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg
      121 catttattat tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa
      181 gctctcatgc aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc
      241 tgatgagcct gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt
      301 tattatcact tatcattttt attacacctt tagatagata cgtcacttat ttatataatc
      361 ctttggaata ctggacttat tttataggta tattggaagg cattattttc tacatcgggt
      421 attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca
      481 tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt
      541 ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca
      601 ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac
      661 atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac
      721 caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca
      781 gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga
      841 cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga
      901 catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca
      961 cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat
     1021 ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta
     1081 acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat
     1141 ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga
     1201 gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt
     1261 gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt
     1321 cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt
     1381 ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc
     1441 ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca
     1501 aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg
     1561 aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt
     1621 cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat
     1681 agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct
     1741 tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc
     1801 ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat
     1861 gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa
     1921 gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga
     1981 tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc
     2041 ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg
     2101 ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg
     2161 ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa
     2221 cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt
     2281 tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc
     2341 gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct
     2401 catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga
     2461 tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt
     2521 gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt
     2581 gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga
     2641 aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc
     2701 tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact
     2761 ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa
     2821 aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt
     2881 attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc
     2941 tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg
     3001 gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat
     3061 tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag
     3121 tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt
     3181 tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg
     3241 ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag
     3301 tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc
     3361 accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa
     3421 taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg
     3481 aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac
     3541 ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg
     3601 aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat
     3661 cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt
     3721 tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt
     3781 aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa
     3841 atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt
     3901 cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc
     3961 aatctgaaac caagaaa
//
LOCUS       DACCZY010000172         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCZY010000172 DACCZY010000000
VERSION     DACCZY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.180"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(716..3865)
                     /locus_tag="IVZ67_002702"
     CDS             complement(716..3865)
                     /locus_tag="IVZ67_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8166721.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DACCZY010000173         3780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCZY010000173 DACCZY010000000
VERSION     DACCZY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.181"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            171..986
                     /locus_tag="IVZ67_002703"
     CDS             171..986
                     /locus_tag="IVZ67_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166722.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1112..1369
                     /locus_tag="IVZ67_002704"
     CDS             1112..1369
                     /locus_tag="IVZ67_002704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166723.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1621..2193)
                     /locus_tag="IVZ67_002705"
     CDS             complement(1621..2193)
                     /locus_tag="IVZ67_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166724.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2209..2814)
                     /locus_tag="IVZ67_002706"
     CDS             complement(2209..2814)
                     /locus_tag="IVZ67_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8166725.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3215..>3780)
                     /locus_tag="IVZ67_002707"
     CDS             complement(3215..>3780)
                     /locus_tag="IVZ67_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8166726.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1243 a          707 c          567 g         1263 t
ORIGIN      
        1 aatttattaa tcgtagttta tataacttat ttgtaagtga ctagagaatt aaataaaaaa
       61 taattactat ctagtacgaa attaattaca taggataata tattaaggtg ctggactaca
      121 agtaaaaaat actgtatgat catgacagat tcaagaaagg agcaataaga ttggttaaga
      181 tagcaggact ttctaaaggt ggaaaaaccg tttcacaact ttataaacaa aactacagag
      241 actggagaca gttcagaaaa aacattaagg attcttactt tattctacca acagaacttg
      301 aaaaatattt accgaataac aaagctatta atttatatct gtattactgt tttaaggcaa
      361 agaatgaagg cggggattcc tggcattcgg tcaataccat agcagagtct ctaggagtga
      421 caactaaaac tataaacacc tggaataaaa ccttagttga tttaggtatg attgcacgta
      481 tagacgataa ccatctcagt aaatcgacaa ttttacttcc tctctctagt tactataaat
      541 tagcccttga tagttcttta aaggatgtta ccgagagcac tgtccatgaa attgatggca
      601 acttggttgc agtttatcat ttgtttgaat ggagaaaaaa tgaggctgat gaaaattata
      661 acgttcctta taacacactc tgttgtgttt ttaaaagaaa aacacaaaaa gatactatct
      721 ataaatttat cttggtccaa gatgagaaaa taagtaattt cgaattaagc actccttcta
      781 gtaagattta tgacgatgca tatagatttg aatgtcaaga tctagaagcc ctcctttccg
      841 caaataatct aaacaatata gaacatgaaa actttgttgt aaacactaga tttaatttag
      901 tttctgaaaa ggactcagat ttgttagatt tgctgataga gcttactaaa aatctagata
      961 agaaagaaaa cattactgta ctataactag catcggtcca tcattaagtt tggactatta
     1021 gaacacttgc tattacagaa aaaggtatgc tagccacgct tactatttat tggtatcaaa
     1081 caagtatctt aatttgaaag gaagatgatt tatggctaat ttatatatag agaacaaagg
     1141 agacttgaat gccattatta ctttaatcgt agttatggta aaatcaatag gtaatatcac
     1201 aataggaatg ctgtttatta tattagcagc agtgctgatt tttatgatag ttatttggtt
     1261 cttaaatgaa agatctaaag acaatcaaca ccgtagggat atggagataa aatcaataga
     1321 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
     1381 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
     1441 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
     1501 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
     1561 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
     1621 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
     1681 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
     1741 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
     1801 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
     1861 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1921 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1981 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     2041 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     2101 cttcttctct acgccgttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     2161 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     2221 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     2281 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     2341 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     2401 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     2461 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     2521 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     2581 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     2641 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     2701 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     2761 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     2821 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     2881 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2941 ttttgcacag tctagttgac ctttgataca cctctatatt tatagagaat ctaaaacatc
     3001 aacatggcat actcaaaatt ctaaatatta gggataaaga attctcctat acaatcaaaa
     3061 atttccttta tttcgaactc cggtcaccta actctattga tctcttacaa gccaaagaat
     3121 atctctatac tacaaagaaa caatagggtg cctggttggt ctctttgtgt tttattttag
     3181 aatctagaac gtaaatattc ttgtcattct gattttatct aagaatatca attaaatctt
     3241 tttttgcagc ctctgaagcg ggcattacag aaaagaccag tccaataacc gaagataccc
     3301 caaccgctag taaaatagta aatagatcaa gtgacacgtt tacctttatc aaagaaccaa
     3361 cactataagc aagagccatt cctaataaat agccaattaa ccctcctgaa agagttaaaa
     3421 tcaaaccttc taaaaggaac tgcagcataa tcgattttcg agtagctcct agggctcttc
     3481 ttataccgat ctcttttgtt cgttcagata cagatatata catcatgttc attacaccta
     3541 cccctgcgat aaataaagat atacctgcta cagctgttat aaaataagtg atcgtactga
     3601 ggatttgacc aattcctttc gttaacatag ccgtatctaa cacttcatat tcccccaaat
     3661 gtttcaaact acctttttct ttcaattggt ttatcacttt agaggtcacc ttgtcaggtt
     3721 caactccttc ctctaaagtt aacgttagag acgatgtatc cttttcagac ttaaaataat
//
LOCUS       DACCZY010000174         3661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCZY010000174 DACCZY010000000
VERSION     DACCZY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.182"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(45..707)
                     /locus_tag="IVZ67_002708"
     CDS             complement(45..707)
                     /locus_tag="IVZ67_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8166727.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            891..2537
                     /locus_tag="IVZ67_002709"
     CDS             891..2537
                     /locus_tag="IVZ67_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8166728.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2528..3610
                     /gene="uxuA"
                     /locus_tag="IVZ67_002710"
     CDS             2528..3610
                     /gene="uxuA"
                     /locus_tag="IVZ67_002710"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8166729.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         1185 a          649 c          743 g         1084 t
ORIGIN      
        1 aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac
       61 tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc
      121 gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt
      181 tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt
      241 tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg
      301 aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg
      361 actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat
      421 ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt
      481 tctgcagaaa caagattaat ttttgaaaca tacgtaccac tttgaggtat cgtatacacg
      541 agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga
      601 tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt
      661 tgtttacgaa tcgcttgata agcttgagat tgcaagctgc ttttcatctt tattcctcca
      721 aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac
      781 attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca
      841 taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt
      901 taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt
      961 ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg
     1021 gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg
     1081 aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt
     1141 accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca
     1201 aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact
     1261 ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta
     1321 ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg
     1381 atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct gcgctacttt
     1441 ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac
     1501 aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag
     1561 gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat
     1621 ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa
     1681 aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat
     1741 ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc
     1801 cgaaattcga aaaagctgga gtattcttga ctgatcgtgc aacggtagat aaagcagata
     1861 cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt
     1921 tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt
     1981 ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa
     2041 agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt cctgatatgc
     2101 ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta
     2161 aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc
     2221 tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga
     2281 gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa ctgggtacgc
     2341 aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg
     2401 gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg
     2461 tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa
     2521 atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga
     2581 tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt
     2641 aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg
     2701 gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc
     2761 ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg
     2821 aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga
     2881 ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata ttccagatga
     2941 tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat tgccgggatg
     3001 ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat cagtagacga
     3061 agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga
     3121 ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattacc
     3181 tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag acagccctta
     3241 taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg
     3301 gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc gaaatattaa
     3361 atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg gttcattaga
     3421 catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga
     3481 tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc
     3541 attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa
     3601 agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct
     3661 t
//
LOCUS       DACCZY010000175         3626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCZY010000175 DACCZY010000000
VERSION     DACCZY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.183"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..478
                     /locus_tag="IVZ67_002711"
     CDS             <1..478
                     /locus_tag="IVZ67_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8166730.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="IVZ67_002712"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="IVZ67_002712"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8166731.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="IVZ67_002713"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="IVZ67_002713"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8166732.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..>3626
                     /locus_tag="IVZ67_002714"
     CDS             3200..>3626
                     /locus_tag="IVZ67_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8166733.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
BASE COUNT         1180 a          586 c          796 g         1064 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcat
//
LOCUS       DACCZY010000176         3554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCZY010000176 DACCZY010000000
VERSION     DACCZY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.184"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(251..676)
                     /locus_tag="IVZ67_002715"
     CDS             complement(251..676)
                     /locus_tag="IVZ67_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166734.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1050..2021
                     /locus_tag="IVZ67_002716"
     CDS             1050..2021
                     /locus_tag="IVZ67_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166735.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2053..3450
                     /locus_tag="IVZ67_002717"
     CDS             2053..3450
                     /locus_tag="IVZ67_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8166736.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1227 a          582 c          737 g         1008 t
ORIGIN      
        1 tctacttttt acataatttt gaacaggata agattcaaat gttttaaatg aatctttgac
       61 tagtttgtga cagttgtcgc atatgctcaa gcttccttgg tcattaaatt ctgtatggat
      121 atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt tcgtctcctt
      181 tacaatcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa agaagatcca
      241 acagacggca ctatagactg tagatgttgc catcctcatc cttcaaacgg taattaccga
      301 tttttgtaaa tatgactgtt gtttcttttt tattttcttt tcctgaaata atatattcgt
      361 catctttggt agtgatcttg atatctgtca gcactgtttt ttcttcagat gattcagaag
      421 aatcagttcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct gtaaatgtca
      481 actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca ctgttggtgg
      541 tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag gattggtcag
      601 agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa ataaatagta
      661 cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta taccattgga
      721 atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa acacccacaa atagagtaaa
      781 ttttagaata aaattttaaa aaggcaaaaa atccgccact ttcagaaagc gttttaaaaa
      841 agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga accggttcaa atacttgttt
      901 aagtataatt aatagtgaga ggaagttgct ctactgaaga ctttagttat atggatattg
      961 atgacaagca acactatgca aagaatgcgg tcatcataat cgaacaacaa gaaagagaaa
     1021 tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat ccgtgatgtg gctaagatgg
     1081 ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg tcagcaactg aaggaaaaga
     1141 acatgaaaaa aatagcagaa gcgatttcag cgttggacta taaagaaaac atcattgcta
     1201 aagggttgaa gaataatcga agcttctcta ttggactttt gataaacggg atttccagcc
     1261 gttttgggtc ggaagttgtc tcagggatcg aaagagtagc tgaagaaaat gggtatagct
     1321 tattattaag cgggtttgcc gatcagccag aaaaaattga acaaaaaatt gaatacctga
     1381 tgaagcatgt gatcgatggg ctgattgtct tcttatccga agaagagtgg tctggatttg
     1441 aaatgctcac aaaaatgagt attccagtta tcgcattgaa ttgtccagaa ggaccagcag
     1501 gtattgatac aatattagtg aatgatcgag aaagtgtagc aaaagtgatc gctcatcatg
     1561 gtgaacaagg acataaaaaa atcggattta ttgctgccac tcaaacagac tacgtagcaa
     1621 gagaacgttt agcaggagcc aaagaagcgg tccaaaatga tccatcgatc caattagagg
     1681 tctttacagg agactattct agaaaaagcg gctattacgg ggcaaaagca ttattagaaa
     1741 aaggggcaac tgctattttt gtcagcaatt acaatatgtc gattggtgcc atcgaatgtt
     1801 tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt cagtcactat gattatttag
     1861 ataaaaacga tctgagtatc cttccgaaaa tcacgatcac gccaccaaca gataaaatcg
     1921 gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa agaagaggaa gaaaatgaaa
     1981 aagtgattgt gatggaaaat atcatacatg gaatcaacta aaacgaacta aaaaagaaaa
     2041 agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt tttggtgggg ttctgcagca
     2101 agtggcccac aaacagaagg aaggatttca ggcgatggaa aaggagagaa catttttgat
     2161 tattggtaca aaaaagaacc gcaaaagttc tttgatcaag taggacctga aaaaacctcg
     2221 caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg gcataattca
     2281 tttcgaacat ctatccaatg gagccgtttg atacctgatg aatctggaac aatcaacgaa
     2341 gaagccgttc gtttctatca tcgttatttt gatgagttga ttgaaaacgg tatcgaaccc
     2401 tttgtgaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg cggatggttg
     2461 aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa gttgttttga attgtttggt
     2521 tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg ttccagtcga agcaggttat
     2581 ttatatcgat ggcattatcc agaagagtca gatatgaaaa aagcgatcca agttggctat
     2641 catgaagcgt tggcttcagc gttagccatc aaatcttacc atgaaatgga gcaaggtggt
     2701 gttattggta tcatcttgaa tctgacacca agctatccaa gagacgaatc aaaccctgaa
     2761 gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc gttcattcct tgatccagct
     2821 gtaaaaggcg agtttccaag tgaattgatt gagatagtaa aagaacttga tctcatgcct
     2881 gtgatccatg cagaggattt gacggttatc aaagaaaata cagtagatct tttaggtatc
     2941 aattattacc agccacgccg ggtaaaagca aaagaaacac caatcgatac taaaaacggt
     3001 ccaatgcctg aggattattt cgacaattat gaaatgcctg gtcgtaaaat gaatccttat
     3061 cgcggatggg agatctatga acaaggaatc tatgatattt tgatcaatgt acgagatcat
     3121 tatggaaata tcgactgctt tatttctgaa aacggtatgg gtgtagaaaa cgaagcacga
     3181 tttatcaaag aagatggcat gatccacgat gattatcgga tcgaatttgt ccaatctcat
     3241 ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggcta tcatatgtgg
     3301 acatgtatgg ataactggtc gtggaccaat gcatataaga atcgatatgg ctttatttca
     3361 gtagacttag cacaagatgg aaaaagaaca atcaaaaaat cgggtcattg gttcaaagac
     3421 gtatcagata aaaacggatt tgaggcttaa gaaagaatag ttgagagcaa atgcgtgaaa
     3481 ttctccattc ttaccatttt gtgaaaaaga agttcaatta tttacgatct ttttcaactt
     3541 ttttgtcttt ctat
//
LOCUS       DACCZY010000177         3495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCZY010000177 DACCZY010000000
VERSION     DACCZY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.185"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..135
                     /locus_tag="IVZ67_002718"
     CDS             <1..135
                     /locus_tag="IVZ67_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166737.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVZ67_002719"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVZ67_002719"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8166738.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IVZ67_002720"
     CDS             complement(2412..2663)
                     /locus_tag="IVZ67_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8166739.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IVZ67_002721"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IVZ67_002721"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8166740.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          972 a          762 c          641 g         1120 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact ctttt
//
LOCUS       DACCZY010000178         3462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCZY010000178 DACCZY010000000
VERSION     DACCZY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.186"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..525
                     /locus_tag="IVZ67_002722"
     CDS             <1..525
                     /locus_tag="IVZ67_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8166741.1"
                     /translation="TACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGEGGLKTELES
                     YINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGVPVIATDC
                     PVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKLRKELTSK
                     NISNIWYEYLNQFF"
     gene            568..1968
                     /locus_tag="IVZ67_002723"
     CDS             568..1968
                     /locus_tag="IVZ67_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP8166742.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILLINFFLQQFNKRL"
     gene            2014..>3462
                     /locus_tag="IVZ67_002724"
     CDS             2014..>3462
                     /locus_tag="IVZ67_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP8166743.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFY"
BASE COUNT         1142 a          443 c          630 g         1247 t
ORIGIN      
        1 actgcatgta gattggataa acaaaaaaat ttgcccttaa tgattgatgc aataggaaaa
       61 cttattcatg aaggatatga gtgtgaactg gatatttatg gagaaggggg attaaaaacc
      121 gagcttgaaa gttatatcaa ccataatgga ttaacagaat ttgttcatct aaaagaattt
      181 acaaataaaa tacatgaaaa gatgcaaaat gcggcaggat ttgtaatttc ttctgattat
      241 gaaggtatat ctaattctat gttagaagca ctggctattg gagtgccagt tatcgctaca
      301 gattgtccag taggtggcgc aagaatgtat ttaggtgata gaaatgaaat aggtcatctt
      361 gttgaatgta ataatctaga taagttaaca gatgcaatgc gtagggtact tttggataga
      421 gaaagtgtgg taagaaaagc gcaaaaagga attaaactaa gaaaagaact gacatctaaa
      481 aatatttcaa atatatggta cgaatattta aaccagttct tttaaccaaa aggaggatat
      541 tgtgtgaaag aaaaaataaa tatattgatg ttttcgttaa cgttagtcgc gtttacaata
      601 ttttgcgctt tttcattaaa tcagaactat tggctaaatt ggcttggaat attgatttac
      661 ttatatgcaa tctgtacatg gcattggaga aaaggagatt caatattttc attatacacg
      721 atttttttca ctcttttttt attatttagt tatgggcaat gcattatgtg ggcattcggt
      781 attggaacag atcgtggaat cggtacaaca gtagtagctt atggtacggg gattattcct
      841 agtgaatcag atatgataat ggtaaaatgg tattcttgta tttctatgtt tgtatttcac
      901 ataggagcac tcttatttac gagaaaaacc acactaagaa aagttagttg gtatgaatct
      961 ggagatgctg atgccgatag aaagttttta tttaaaatag ggtgtatcat ttctataata
     1021 gtagtaccga tagtttttgt ttctaaatta ctagaggtac gtatagcatt agtccatggt
     1081 tataatgcat tatattatgg tgattatgct acacaaagtg gatatttaca aattattcta
     1141 tatctttttt ttccagcctt aatatgttta ttaattggta gcaacttttc aaaagtaata
     1201 acgagatttg tttttattat ttttgcttta tattctttat taggaattat gtcaggagac
     1261 agaggaagtt ggttgtatag ctctattata ctcatatggg catacactca atataaagga
     1321 acctttaatt ataaaaaatt aataaagtgg cttattttag ctgttatagg aatatatatt
     1381 ctaaacgtaa taacgaaagc acgtgatggt ggaggactaa gtaaacttac tttaaaagat
     1441 tttacatctg tatttgattc agatgattct cctttagttg attctttttt tgaaatgggg
     1501 gggactatga gtattataac ttttttccta ataagtggta atggaatata tccctttgct
     1561 aatacttatt tgacctcact cttaggctct atatcaacaa gaatgctatc aatgtttgga
     1621 ataaagtttg ttttattagc tgattggttt tctcaggatt atttaggaat cacttggggg
     1681 actggattct caatgattgc agaagcctat gtcaatggtg gatacattgg gggtatagtc
     1741 tatattttta ttattggtat agtcatcggt aaaatccttt ctaatagtat agacaaccaa
     1801 gatattgaaa atcatcctat gaagatgttt ttggctataa ctacagcaaa tattataatg
     1861 ggttttccta gagctgcttc atatttaatt gtgaaaaatt ttgtgtatgg tgttttaatt
     1921 atattgttga taaacttttt cctacagcaa tttaataaac gtctttaata tttaagctta
     1981 ataaaaagat aaaacaagtc aggagttgca aaaatggaaa gaactgctcg gctcaaaaaa
     2041 aacactattt gggctctggt gtttcaaata acttctattg tctgtggtat gatcttgcta
     2101 cgtttgatat taacaaagta cggatccgag gttaatggaa tgattagctc tattaaccaa
     2161 tttctacaaa ttatttcttt tttacaatta ggagtaggcg ctgtaatgca atcaactcta
     2221 tacaaaccac tagcagaaaa ggataattta atgataagtc aaatcttagc ttctggaggg
     2281 aagttttatc ggcaaattgc atatattcta ctagtatacg tgggtttgtt acttgcagtc
     2341 tattatgttc tcgttcctaa tgaatttggg agatcatata caatggcaat gattgcaaca
     2401 ttaagtatta attcatttgc tcaatattat tttggtattg ttaatacttt gttattaagt
     2461 gctgaccaga aaatttatat tacgtatatt gcacagacaa tagcatttat ttctaataca
     2521 gttgcatgtg ctttattaat tagtctgaac acagagatcc ttatggtaaa acttattact
     2581 gctataattt atcttgcttg tccaatatat tatagatggt atgtggatcg aaactataat
     2641 ataaagagga atgtgtcata tgataaggaa ccgatccctc aaaaatggaa tggggttgca
     2701 caacacgtag cttctgttgt actagatagt actgatgttg tagtgttatc tatattttct
     2761 aacctgatat atgtatctat atataatgtg tacaatttag taatatacgg agtaaaacag
     2821 ctattcatgg ctatgacaaa tggcgttcag gccttactag gggaactttg ggtcagaaaa
     2881 gaaatagaag ttctttacaa aacatttgca tggttcgaat ggattgtaca tactttgaca
     2941 gtttttattt ttggctgtac cggtatacta atcgttccat ttattcaagt atatacaaga
     3001 ggagtaacag atgctaatta taatcaatta tcattcgcag ctttacttgt aactgctaac
     3061 gcacttcact gtctgcgtac accttatcat gtaatgataa aagcagctgg taaatataaa
     3121 gaaactcaaa atagttttat aattgcttct ttgttaaatt taggtttatc attaattttg
     3181 gttaaaccac tagggctagt tggagtagca ttaggatctc taatttcaat cacatatcat
     3241 acactttgga gtgcaaatta taattcaaaa tatttgctta aatggccgat gaaaaaattc
     3301 tataaacagt tatttgtaaa catcttaact gtagttcttt cttttggggc gacatcacaa
     3361 ttgaaaatga tgtcaatcac atatttggat tggattgttc tcgcaattaa atgtgcagtt
     3421 attgtattta tatgtataat cctagttaat gttttatttt at
//
LOCUS       DACCZY010000179         3445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCZY010000179 DACCZY010000000
VERSION     DACCZY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.187"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..948)
                     /locus_tag="IVZ67_002725"
     CDS             complement(<1..948)
                     /locus_tag="IVZ67_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HAP8166744.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNS"
     gene            complement(1232..2179)
                     /locus_tag="IVZ67_002726"
                     /pseudo
     CDS             complement(1232..2179)
                     /locus_tag="IVZ67_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2191..2664
                     /locus_tag="IVZ67_002727"
     CDS             2191..2664
                     /locus_tag="IVZ67_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8166745.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(2837..3391)
                     /locus_tag="IVZ67_002728"
     CDS             complement(2837..3391)
                     /locus_tag="IVZ67_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8166746.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1118 a          629 c          533 g         1165 t
ORIGIN      
        1 agagttgtta tatatccaat gcgatttaaa ggttaaatga tatttcttta atgtatcgat
       61 ataagcttga tgtcgttctt gaataacact tggttcacct acaatgtagt caaaagaaag
      121 aaacgctatt ttctcatgac cataatgaat caaatgttcc actgccaatg tactagcttg
      181 gtaatcattt gtatttacaa ataaacgatt catttgttgt tcaagttctc ttaatgtctc
      241 ttttgaaaga tgcgtatcaa tataaacaac aggaccatca aaaacgttat ttaattccaa
      301 aaattctttt acacgtacac caacaacaat gaacccctca aaacgccaat ttctctgaat
      361 agttttaaca tcttctgctg aattaacatt atgcaccaat acaaaataac catgattctt
      421 tgcctgttcc tcaattccag acaaaacttc tgcggtaaat ggatcagaaa atacaaaatc
      481 agcattatca atagccgaaa aataaagcaa tcctattaaa aatgcaatcg tcttaccgat
      541 tccctgacag acagaacaca ttccactagg atcattaaat gaataagcat ttgcatatcc
      601 taaactaggt gttgcaaaac atgaaaacaa cacacgaaat aacgcattga tgttagtaat
      661 cgtccctaac gttgagcgcg cactgccacc aatcttcttt tgattgacca caatcgccgt
      721 agaaagatta tcaatctcat caacctctgg acgactgtat ttaggtaaaa ataaacgggt
      781 aaaactatca taggtgtcat tcagttgacg accagcttct tgggtaatag tatcaaaaac
      841 aatcgatgat tttcctgaac cagatacgct ggtaaaaata ctaattttat ttttgggaat
      901 tttgagactg acatttttaa gttgtttctc ttgcgtggat aatttcaata tactcgttca
      961 tttggtactc ccctttattt tgaaatagtt gagaatgttt caatgagaca cggtattttt
     1021 ggcagatgtt tagtttacct agaaagaggt ataaaaatca agaaaaaaag agaggctgac
     1081 tcataagtag ttttattagt cgaaaatgat agtttataaa aataggaacc gcatcaaatc
     1141 aacattcttt agaatggatt tgatgcgttt ttctttattt tttgacttat gagtcaacct
     1201 cttactcttc gtcatggatt tgattagcta attattgctc cacttgttca cgaatgaaat
     1261 ttaagtactg cggtaaaaat aaaggaattt cttcaaagcg tggactttga ttttcatacg
     1321 cttttgcatc taggtttcct tcccctgtag caatttcaat caataattga tttgggtcac
     1381 gataataaag tgatacaaaa aattcacgat cttttattcc tgaatttata aaattgcgct
     1441 cttgtaattg tttgtcgatg cgttgtaaat cagatttatt ttctacgcca aacgcaacat
     1501 gatggacgct accaatccct tcaatggcaa ctggattctc acgatcttga ataatctgaa
     1561 cttcttgata gaactgagtg tcttgatttt ctaagattgt cactcgttga tgacccgaaa
     1621 agaaactgac gtattctttg tgctaccagc ctaccgacaa taactctcgt tcagtcgcat
     1681 cggaatattg tacgcgtagt tgaatggcat caattcctaa aatggcatgc tctgtaggga
     1741 tctggtctgt gacatacggg aaataatcat caatatcttc aaattcgcgt aagggaacta
     1801 aagctaatcg tgttgcatct ggcccttcaa aacgtaagat aggtcgtcca gaaaatgatt
     1861 cgattccata atggcaaacg ccttgtgttt caaaatatat ttcccaaaaa tgcaatgatg
     1921 cttcggaagg aacttttagt atcgttcgtt caatcgtatt ggttccaaaa aactggttgt
     1981 tcccttcttg tacttcaaaa aaagtcagtt cagttcccac acggtggtga ttatctgaaa
     2041 aaaacaggtg atacatggta tgatcgtctt gattaattgt tttcattagt aattttaaac
     2101 cgagtatatt atgataaaag tcaaatgtcg gtcgaataaa gcgattcaca atagaaatgt
     2161 gatgaatacg tgcgggcata atatctaaaa atgagaatca aacctataaa agatggtgaa
     2221 caacttttgt tttcatcaga agaaaataag cccctttatt tagactatgt aaatcacaat
     2281 ttgaaaatca tcattaaaga gaataactta aaacatatca cacttcatgg ttttcgtcat
     2341 acccattgta gtctgctctt tgaatctggt gcgtctgtca aagaagtgta tgttcgactt
     2401 ggacatacag atattaaaac cactatggat gatatataca cccatatcgc gaaaaagcag
     2461 acagaggaaa cggcaattcg ttttgcagat ttcatgagtt ctacagagga aaagcaagga
     2521 agtttgcaga aggtcaaaag tatggtcaaa aacgaaaaaa gcagactatc cctattagat
     2581 agtctgctaa acgttgagtt atcagtattt ttcttattcc gcagcgtttg cgtttttgag
     2641 accgtattga ttctcttttt ttaatttcat ccttttccaa cctaaaaatg ttatttaatt
     2701 acttagttta tataacacga acctatacca agtaccagta accccagctt ttattccgcg
     2761 aactttatga aataaaatcg ttctagataa aaatagcttt taatctttgt ctttactgtt
     2821 tataaaaatt tttaaattat acacctagtt taaatccacg aagtgatgct cctatttcat
     2881 cttcagttat accaatgtac ctttttgtaa ttgcttgcga gctatgatta aaaataaaca
     2941 ttaaggtagc aatgtctcta gttttcttgt agtagtgata cccaaaagtt tttcttaatg
     3001 tatgtgttcc tatatcatct cgtcctaatt tttctgctat atctgcatat atctgataga
     3061 cacgatccct tgttatatgt cgaccttgtt gccgactcgg aaataaccaa tcattttctt
     3121 ctttaccttc agtatataat aaaatttctt ctctcaaatt atctataaaa atcggttttc
     3181 gcttacctgt tttatgttca acaataatag gcttactttt gcctcgtata tctccaactt
     3241 ttaatcctat aatatctgac atacgtaacc cattattgat tccaaacaaa aataaaaaac
     3301 tatctctttc cggattactt gttcttctta gccaaaactg aaaatcttgt atctcttcat
     3361 ttgtccttaa cggttgtaca ttatagctca ctattgtttc ccttcttaaa aatattctga
     3421 gttaatgatt atgtatatat tttac
//
LOCUS       DACCZY010000180         3430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCZY010000180 DACCZY010000000
VERSION     DACCZY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.188"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1299)
                     /locus_tag="IVZ67_002729"
     CDS             complement(<1..1299)
                     /locus_tag="IVZ67_002729"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8166747.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIF"
     gene            1484..3430
                     /locus_tag="IVZ67_002730"
     CDS             1484..3430
                     /locus_tag="IVZ67_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8166748.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1081 a          609 c          582 g         1158 t
ORIGIN      
        1 aaaaatgtta gcagttgttt ctttagtatc aattgagcat tcacgaatcg taccaaagtc
       61 agtggcatat tgaatgcctg ctttgctgcg atctaaaata atttttcctt ctttggtatc
      121 tacaatcaag ctaagtccat ttcctttact atcagagaaa agaagtaact ccgatttctg
      181 atttggagga aaagttagtt caagctcata agtattattt gtttctgctt ttgctgtaaa
      241 ttgttcagaa ctagcacgta atgatgtaat agcttctact ggatattggt aaagtttacc
      301 atcttttatt gaaagttctt ttacaagact cattgctccc tgataatcat acttatcagt
      361 tggataatcg atatcaggta gtccaatcca actgacaatc agagcacgac catcaggagt
      421 attgaatcct tgagtcgcat acgcttcaaa tccataatca agattttgaa tttctgaagc
      481 gcccacaagt tttacttttt ctatgtcaag cgattgacag atcttaaaag tattcggata
      541 gatattccca taatttagct cagatttatc caacccttga ggacaataaa tgagaacagg
      601 ttttttgtct acaaatacta gatttggaca ctcaatcatg tattctgaac cagtgccgcc
      661 aaaatcaagg ttgccaactt cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag
      721 tttgataaag cctgattttt ctaaattttg tgctccaaca atcgcataca attgtccttc
      781 ataattaaac atttgtggat cacgaaaatg atcagttaca tcagcaggtt tatgaataag
      841 aacattctca cctttcgtaa tgttgtagtc tttatccatc catgctccga tttgaagtgt
      901 atcacgttcc caattttcat ttcgaacgtt tcctgtatag agtaagaata gattcccctc
      961 tatttcatat gcagaaccgg aataagcgcc gtggctatct cgttttgtgt ctggaagaag
     1021 ttctgctcct gttacatgaa aatgaactaa atcatctgat tcattgtgta cccattcttt
     1081 caacccatga gcagctccaa atggccaatt ttgataaaaa agcgtatatt ttccgttaaa
     1141 aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta ggttcgatat gatagctagc
     1201 acgccaaggt gatcgtttta cattatcctt aattttatcc atctcatctt tagtccaatc
     1261 agaataagca cgatatcgta tttctcgagg taagttcata ttttactcct tcaatttatc
     1321 tttttacaac tagtatggta aacgttttcc gttgaaaaat caatcatttc atttaaaaaa
     1381 ataaactata tgacaaacgt ttgacatatt tttaagatat gctaaaataa atttgcattt
     1441 cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt
     1501 agcttccgac gtaatacaag cggtcggaaa agataacctt attgcagctg cacactgcgc
     1561 tacacgtctt cgtctcgtac taaaagatga cacgaaagtg aatcaaaaag ctttagacga
     1621 aaatccagat gtgaaaggca catttaaaat tgatggacaa tatcaagtga ttatcggtgc
     1681 aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa actggacttt cggaaatttc
     1741 aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa ttcaacccca ttatggcatt
     1801 aattaaatta ttatcagata tttttgttcc aattatacct gcccttgttg caggtggttt
     1861 attaatggca ttacaaaact ttttaacagc tgctggactt tttggaccaa aatcaattga
     1921 agaaatgtat cctgcgattg aaggtatttc ctcaatgatc caactgatgt ccgctgcacc
     1981 attcatgttc ttaccaattt tagtaggtat ttcagctgct aaacgttttg gagcaaacca
     2041 atttcttggt gctgcaattg gtatgatcat gacaacacca gatcttggtg gctcaactga
     2101 atattggaat atttttggtt atcatgtttc acaaacaaac tatgcttatc aagttattcc
     2161 agttttagca gctgtttgga tattatctat attagaaaaa ttctttcata aaaaattgcc
     2221 atcatctatt gactttacat tcacgccatt attatcagta atgattactg gatttttgac
     2281 tttcacagtt attggccctg taatgttatt gttgtctaat ggaattacag acattattgt
     2341 ttggctatat aacactactg gatttattgg aatgggtatt tttggaggaa catattcact
     2401 tatagtcatg actggtttac atcaatcatt tccagctatt gaaactcaat tactttcagc
     2461 gtggacaaat ggtatcggac atggagactt tatctttgta gttgcatcta tggcaaacgt
     2521 tgctcaaggt gctgcaacat ttgccatttg gttcttaact aaaaattcta agacaaaaag
     2581 tcttgcttcc tctgcgggtg tctcagcact tcttggaatt acagagcctg ccttgttcgg
     2641 agttaactta aaataccgct ttccattctt ttgtgcttta attggttcag gaattgctac
     2701 agcagtagca ggattactta aagtagttgc tgtttcactt ggttcagcag gtttcttggg
     2761 atttctttca attaatgcaa catctattcc attttacgta ttgtgtgaat tgattagctt
     2821 tgtagttgct tttgcaatta cttacttcta cggtaaaaca aaagctgctg gtatctttaa
     2881 agcagaagaa attgctgaac aaacaccagt taattcttca aaagtagcaa caacagatcc
     2941 aatatctact gatgttgaat caacacaaac aaaagtttca tcagaaacaa ttgttagtcc
     3001 catttctggt aaagtgatta gtctttcttc agtaaatgat ccagtctttt catcagaagc
     3061 tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg gtccattctc cagtagatgg
     3121 tactattcaa ctcacgtttg aaactggaca tgcttatggt attaaatcaa ataatggtgc
     3181 tgaagttcta attcatattg gtattgatac tgtatcaatg ggaggaaaag gatttacaca
     3241 aaaagttact actaatcaaa aagttagaaa aggcgatatt cttggagcat ttgaccgttc
     3301 aatgattgag aaagaaggtc ttgatgatac aacaatgatt attgtgacca atacttcgga
     3361 ttattcagaa gtaaatatag tagctgaaaa tacagtcact gagggtactg atttacttga
     3421 aatcaaataa
//
LOCUS       DACCZY010000181         3385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCZY010000181 DACCZY010000000
VERSION     DACCZY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.189"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(558..833)
                     /locus_tag="IVZ67_002731"
     CDS             complement(558..833)
                     /locus_tag="IVZ67_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8166749.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            940..1704
                     /locus_tag="IVZ67_002732"
     CDS             940..1704
                     /locus_tag="IVZ67_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8166750.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1827..2330
                     /locus_tag="IVZ67_002733"
     CDS             1827..2330
                     /locus_tag="IVZ67_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8166751.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2609..3256
                     /locus_tag="IVZ67_002734"
     CDS             2609..3256
                     /locus_tag="IVZ67_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8166752.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         1141 a          595 c          686 g          963 t
ORIGIN      
        1 tttgaaaaaa gacaaaaaat caagaaagaa atacttagaa agaaaagagt gattccaagt
       61 gtttctttct tatttaaaaa actaaaaact tttttgttct gtctggaaaa tatgggttaa
      121 gcaaattcat tgcaagttat agatcagctt ttttactacc tgtctttaat acttgaggca
      181 aagcgcctca aaaacaacct tatcgacggt gttcaaaagc ttgcatctac ggcattaagt
      241 ccagcctgtt gaaaaatatg gagagctatt tatcacaacc agtccttaat gcttgatgca
      301 gaccagcttt ttcacaacct tattgacggt gttttatcag ctgcctctcg gtattaagtc
      361 attacatttc aaaatatgag aagctatttt gaaaatgttt ctccttaata ctcgaggcaa
      421 agcgcttcaa aaacaacctt atcgacggtg ttcaaaagtc agtttctcgg tattaagtca
      481 aatttatgca aaaatgtgga aagccatttt cacaaaattt tccttaatac tcaaaactaa
      541 gcgacctttt cacaacctta tcccactaca tcaaatttct ttcgttcctc aatcatcgga
      601 tcttcttgga catccacata ctgcactcgt ccacttgggc tcggagactt cttgacttct
      661 tcgataaatt tttggatgat gtcgtcattt ccgattgctt cgattgtgac tgagccgtcg
      721 tcttcgtttt ttaccgttcc ggtcacgcct aactgatcag caaccatctt ggtcatatag
      781 cgaaatccca cgccctggac acgtccttgg acattcattt taactttgcg catgttcgtc
      841 acctttcttt cctgttttat aaatcaatga taccgatttt tttaggatga aacaagctca
      901 agcacctatt ccatgatata atcagttgag gtgaggaatg tgaaagagat ccaatcagca
      961 aaaaatccaa gaatcaaaga attaaaaaaa ctacataaaa agaaatacag agaagaaaag
     1021 gcagaatatc ttttggaagg gttccatctg atcgaagaag ctgcgtcgtc taatgcagaa
     1081 ataaaagaac tttttgtgac acagagaggt atgaaagaat ggggagactg gctggctgta
     1141 aacaaagaga tttctacttt tctagtttca gatgaggtca tgaccgctat gtcagatcta
     1201 cctacaccgc aagggatctt agcagtgatg acgttaacat ctgaagatac gccagatttt
     1261 tcaggaggct ggcttttact agataatgta caggatcctg gaaatgtggg aacgatgatc
     1321 cgtacggcag atgcgtttgg tatggctggt gttttattag gtaaaggaac ggcagatatt
     1381 tatagtacaa aggtgctgcg aagcatgcag ggaagcaatt atcatttgcc tgtgttacaa
     1441 aaagatttgg cagatgtaat ccctgtaatg aaaggcaaag ggtttacggt gtacggtaca
     1501 gaattaaatg aagaagcaca tgcattagac aaagtagaaa aaacagagga tttcgctata
     1561 atcatgggaa acgaaggtca gggagtaagt caggaaatct tgtctttgac tgatcagaac
     1621 gtatatatcc caatgaaagg aaatgcggaa tcattgaatg tcggagtcgc agcagggatc
     1681 gttctttatc atttttcttt atgattaagg aactattaag ctttagctat tttatctaga
     1741 gcgactagaa taatccgaac actggtggta tacttagatc aatttcagaa gaaaatgaaa
     1801 ttcaaaaagg tggcagatcg ataattatga cagaaaaatg gcgtgaagac gaagaatata
     1861 tgtcttatgt acaagatctt ttagaaacag aagcagtgaa aaaattagcg aattacacac
     1921 agcatgttca ttccactcgt ttggagcatt ccatcagtgt ttcttactat agttatctgc
     1981 tcgcaaaaaa atggggtggc aatgctaaag caacagcgcg tgctggactt ttgcatgatc
     2041 tgttctacta tgattggcgt acgacgaaat ttgacgaggg gacacatgcg tatatccatc
     2101 caagaattgc tgtaaagaat gcagaaaaaa tcaccgattt gtcggatctt gagcgagata
     2161 tcattttgaa gcacatgtgg ggtgcaacga tcgcaccgcc taagtataaa gaaggatata
     2221 ttgtgacatt cgtggacaaa tactgcgcag tcaaagaagc ggctcagccc atgtctgcaa
     2281 gtatgcgtaa gagatggcaa cgttactttg ggaaagaatc ctcgatttaa taggtaaaac
     2341 caataataat agactatatt ccgagaaaat ctggctgatt ttctcggttt tttctgtttg
     2401 cagttgacta tctgttcaag atacggttta cttttattta tcgaatgaat catgtacata
     2461 gaagaatgaa cattaaaggg ggtgatgata agatgcaaaa cgtatcttat ttaaaagagt
     2521 ccgggatctt caggtaacac atactttgca gaagatcact tttttctaca aagtatgcac
     2581 tttggttaga agaaaggact ttaaaatcat ggaacaaaca ataaaaccat accaatattt
     2641 ttttataaaa agagaagtag atcagttatt aaatgcgtac tcatctgtca atgatccaaa
     2701 aacggtacaa acagttcagg cactagcagc tgaaaagatt cgagacatat tagaacacga
     2761 actgccagaa atcgatacat ttttaacgac agttttagac gtaaaactga caaaagcgca
     2821 agcagaaaga gcattggagg agctgaagca aatcgtccag ccgtttatgc agcctagcaa
     2881 accacaaatc gagaaattat tccgaaaagt gaaaaaactc aaacaaccag cttggaatga
     2941 aatcgacttg caagaacata cgtacatcgg ctggaatgat ccaggtacgc agaaaaaatt
     3001 catggtctat tacgaagatg aaaaattaaa agggattagc ggtactttat caccaacgat
     3061 aaataaggga atctgtgcaa tctgtcagaa aacatcgaat gtgtcgcttt ttctttctac
     3121 aacgaaagca ggttctgatg gcacgtatac gaaaaagggg aattatattt gtcatgatag
     3181 tgaccaatgt aatcaacagt taacgaaatt ggctcctttc tatgaatttg ctgaacgtgt
     3241 aaagaaagaa cgataatttt attgtttttt acagaaaaat atgctatctt ttaatggaag
     3301 tgaataggtg atggtgttcg cctttaaatg ctaacgaaag atagctaatg acgcctacta
     3361 aagtctgggg atagcccctt tcttt
//
LOCUS       DACCZY010000182         3316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCZY010000182 DACCZY010000000
VERSION     DACCZY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.190"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..308
                     /locus_tag="IVZ67_002735"
     CDS             <1..308
                     /locus_tag="IVZ67_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166753.1"
                     /translation="KNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQ
                     LMGDGKSELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            322..1011
                     /locus_tag="IVZ67_002736"
     CDS             322..1011
                     /locus_tag="IVZ67_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166754.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1136..>3316
                     /locus_tag="IVZ67_002737"
     CDS             1136..>3316
                     /locus_tag="IVZ67_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8166755.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDET"
BASE COUNT         1112 a          447 c          707 g         1050 t
ORIGIN      
        1 ccaaaaatat ctattctatg tcagatgaag aattgtataa agaatttggt gatttatccg
       61 gggaacagtt agaagaaagt attccaggaa ttagtgactc agataaaatt gttgtcttgt
      121 ctgctggtgg gcaattaatg ggagatggca aatccgaatt agttagttat aaacaaaatc
      181 acagacagtt tattgatgtt aatactgatt ccaacgctat ttcgattaag gatctaaaag
      241 agcttttaaa tagtaaaaga aatgaattaa agcagataaa agaaactaat tcagtggaat
      301 tagagtaaag gaagattcat tatgaaaaag atttttggtg tttctgttgg gctacttatg
      361 atgttaacat ttggaaatgc agtatatgct gctgagaaag aagttatttc agatccagag
      421 cagtacgcct cacgatactt tgctgatttt gaagaagaag ataaagtact tgcattgcca
      481 gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta tggataatcc agatataatt
      541 caaggttcat ataattcaga aactgatcct gcttcagttt cagtgacaga agcaaaaaaa
      601 attgttgctg aaaaattcaa aaatgatatt gaaactactc caattatgcc tcgaggagca
      661 tttccacccg atgttggtac tagcggacac cttcttgtac tagcaggagg aggaagctat
      721 aaatcacagc catttagtgg tgcgggatgg cgttttggag gtatgggtgt aaaaccagct
      781 gatggtacag gcaacttttt acgttggaaa tcctacattg acgatggaag agtaggaaat
      841 gtaaatcaag ccaacgcaac taaatctggt tctattcaag gaatggcatt gtatgctcgt
      901 gaaggattca aatactttgc accaaacggg cctggttgga caatgaatat aggtatttat
      961 tacacttata atcctgttgc aggtacgtat tatgttgttc aaaatattta ataaaacagc
     1021 acaaagagat ttctttgtgc tgttttatta tttttagttg ttgacaaaca ctaaaatata
     1081 aaatataatc aacacataaa cgaacgtatg ttcggcaaga aagcagggga tttttatgga
     1141 tgagatggtt ttaagtactc agaaatggct aaacaagaaa tacagtaatg ttacagggtt
     1201 tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca atctatggac ttattgaagg
     1261 actacaagta gaacttggta ttactaatct agtagctaat tttggaccta ccactgaaaa
     1321 aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt
     1381 tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca ggtggcttcg atggggtata
     1441 tactccatat ttagatactg ctgtaaaaga gttgcagact gatgcagggt ttaatggaag
     1501 cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt
     1561 tgttcttgtt cccggcggag atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa
     1621 ttatttagaa tacacgggaa taatgccttg tgatggtatt tatcaaagaa attcaaatca
     1681 agcattaatt tttgcattac aagctgaact aggatattct cccagtgaag caacagggtc
     1741 ttatggtaat gggacaacta gtaaaactta tcctgtgtct gaaggtaact cgggaaacta
     1801 tgtaagaata attcaatatg gattatatgt taatggttat tttgaagacg gtacattcga
     1861 tggtattttc actaaatata tgggtttaga agtactggca ttccgaaaat ttatggtatt
     1921 gcctgaatat actgaaatag cagatgttgt tgtaataaaa ggactattgt ctagcgctgg
     1981 agatattaga agatctgcag atggcgcaga tacttctacg caattaacta gatctcaaat
     2041 ccaaacattg gttgataatg atataaaaat agttggtaga tatcttactg gtactgttgg
     2101 aattggaaaa gatgaacgaa ataagtatct aactacagag gaattgaata atattttttc
     2161 taaaaattta tctgtgtttc ctatctatca agatggaggt gcagcattag cttattttac
     2221 ttatgatcgt ggcttatcag atggtaagaa ggctatagat gcagcaaaaa atctaaatat
     2281 accattaaca acagttattt attttgctgt tgatctagac atgttagggg aagaaattct
     2341 tgcatatgct ggagaatatt ttaagggggt atctgctgta atgtcatata gcgggtatca
     2401 aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata atcaataacg ggtatgcttc
     2461 gtttgcattt gtttcagata tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc
     2521 tagacaatgg tcttttgatc aatttgttga atttaccata ggaagtggca acggagctgt
     2581 tggtatcgat ttaatcgcta catcgggtag agatagcgga tttaatgagt tatcaaatga
     2641 tactaataat gataattaca ttgccaaata caatcagaag gttataaatc agatggtacg
     2701 tgcgtatcaa tatctatcac aagctggtct tgataatcct tggaaccctc atttgacatt
     2761 ttatagatat gtaaattatt ctggtttgtc atgggacatc atttcttctc ctgtaacaga
     2821 gcatgataga aaaatttatg atgaatatag gaataaatta actaatagcg agggcctgta
     2881 taattatttt atagatccta atactggttc aataataggt ttgcctcact taattgtgac
     2941 gttacaatca cagatgtttc tagccgacac aatcaatgat agtgtttctg actttgcagg
     3001 ttggttagga gatttaatga cttgctgggg tgaagttaag aaattaggaa tacaaatgga
     3061 gcaaggtgta tttgcattag taggtacggc tgcaggtgga acatttagtc tagaagattt
     3121 ataccaagat gttgacgcgg taaatgttta taccttagca aaaagaaatt ctgattcaac
     3181 agctttatcg gctttaaaaa cgtactattg tgataagggt gctattgata gatttaatca
     3241 atttgttaag aacagatttg gttcttcaga taaaatcgct tcagtaacaa atagtttatt
     3301 agaaaatgac gaaaca
//
LOCUS       DACCZY010000183         3250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCZY010000183 DACCZY010000000
VERSION     DACCZY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.191"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(553..2166)
                     /locus_tag="IVZ67_002738"
     CDS             complement(553..2166)
                     /locus_tag="IVZ67_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8166756.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="IVZ67_002739"
     CDS             complement(2307..3215)
                     /locus_tag="IVZ67_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166757.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1046 a          558 c          573 g         1073 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt
//
LOCUS       DACCZY010000184         3242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCZY010000184 DACCZY010000000
VERSION     DACCZY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.192"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(119..811)
                     /locus_tag="IVZ67_002740"
     CDS             complement(119..811)
                     /locus_tag="IVZ67_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8166758.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            896..1072
                     /locus_tag="IVZ67_002741"
                     /pseudo
     CDS             896..1072
                     /locus_tag="IVZ67_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1248..1817)
                     /locus_tag="IVZ67_002742"
     CDS             complement(1248..1817)
                     /locus_tag="IVZ67_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8166759.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1891..2214)
                     /locus_tag="IVZ67_002743"
     CDS             complement(1891..2214)
                     /locus_tag="IVZ67_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166760.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2368..3180)
                     /locus_tag="IVZ67_002744"
     CDS             complement(2368..3180)
                     /locus_tag="IVZ67_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166761.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          901 a          651 c          570 g         1120 t
ORIGIN      
        1 caaagtgaac tgaaccccaa aagttgaact atttaatgga ctgtttccga tattctactg
       61 gagatagtcc attaaatagt tcaacttttg gggttcagtt caaagagggg gggatttttt
      121 atcgttgtgg aatatttaaa taccaacgct tgtcatgaaa atcttgtgct ccacccttag
      181 tatttccttc tggatcgttt gtcgcacgca tcatgacgta tactttctta ttagggaaat
      241 cacgcatatt gaaagataca tgatagccaa catttcctaa agtattataa gcttgattta
      301 cgtctggtct ataaatccca tcagctctta ctctagctag ttcttttcca gtattgtagt
      361 ccataatgaa aatatactcg tatttataat tagcaatgtg ccatccagct acatgcaagt
      421 ttgcgttttc gatttctccg aactgatcaa tgtgggcgta atttgttcca tctgtcagcg
      481 tagaatttgc agcacctgct cgagttggat caattactgg tttatcctcc gaagtagttg
      541 gattatcatc cgtaaaacca tgagccaaat cataggctag tttttcttta cttacaccca
      601 tttcagaaag ataaccgtaa ggatctgtat gatcgcccca aatattttgt gttacccata
      661 aatgcgattt gattcctggt tggttataag gagtgtctaa tgttaatgga ataccatatt
      721 tcattgctga atctcttgcc aattcaacgt atactttata gttcttttca aaagtttctt
      781 tatcatgtgt atgttgtaac tcgatttgca caggactttt ggctcacgag gttgtcctac
      841 tctacatctt gctgatagat atgctgaaag gaaaacacca acattatgtt ttgctttttc
      901 agctagaacg agacaagcag gaataccttt agaagatatc aaaggttttt taggtcataa
      961 agatgtttcc actacacaag tctatgccca tatttctccc gaagtgaaaa aaagatcaat
     1021 gaatcaacta gaaaactata tagaagagca aataaaaaag cactcaaatt gagtgctttt
     1081 ttaagctcac tatcactaaa actatcacta attttattta taagcgaaca tgtttctatc
     1141 actgattttt taaatctagc gaaaaaccct tatcttaaag gcgttcgttc aattctttgg
     1201 caaggtcttc aaagcctggc ttgcctagaa gtgcgaacat ggttttctta tagctcatgt
     1261 agaaaaattt tcttgatgat gattttatcc atcaattgag attgactgtt ttgctctcct
     1321 atgatttcag ctatgattgt ccgattttgc ggttttcttc ttaactggta gttgattttc
     1381 ccagtttcca tcaactcgct aagcatatcc acgactccgc caaatccggt ctttttcgta
     1441 ataaaaccta gtgctaccat tcgttcatct agattttttg ctgtaatcgt cacaccagta
     1501 atgagcgagt gctgtaatgt ttgggccagc tcttccatcc cttcagcatt ggatacgacg
     1561 gaagctgttt tgttaatagg ttcttcgttt atttcttttg gttctttcct ctccactttc
     1621 actgtttgac gaaggacatc tagttcttgc aagagcgttt cgtattgttc ttgggtcagc
     1681 atcacgccag ctactttttc acgattaaat atatatacgc cagttcccgc gtctcgcgct
     1741 tgagcaaata catccatcgg cgatcgtttc acttctgtga tcgaagatgt gggaacttct
     1801 aatttcttta attccaatgg atttcctcct ttactaatcg ctttttatta tagcacagac
     1861 tgacttgttt tgatcttttt tccaaaggtt ttatcggttt ttgcctaacc agtaattgat
     1921 atcggtaaga agtctttcga taccaaatga aggatggtac agatacgttt tttgatcttc
     1981 gttatggtct agtggaaaaa aatgatcgat gatcactaga tctgcatacc gaggcggctt
     2041 atttcgagta catacatgta tagagatccg ttttcgatgt cttagcgttt tgttcagtac
     2101 cccagcaagt cttcttgctt cttggtgttc atagcgaaaa aaaaagacga ttttcacttc
     2161 aggagggtgg acatctaaat accctcgttc ctggagtgtt ttcagtaaaa gcatccccca
     2221 ctctactgcg gggtgtgcaa gtgcttgttg gatcgctagc cgaaattgat atagtttatc
     2281 tttggaattg ggaaattttt ctaatgtttc ctgataataa cgaacttctt ctatcagccg
     2341 gtcatggata ccaattacgt aacgaagtca ttcttggtaa agtacttttg tcagctttga
     2401 tttgctttcg ctctcaaatt cgctattcag ccaaaacagc gaatcaagct tttcagtcac
     2461 aatcgtcaat ttccgatagt cttgtgtctc ttctatccgt ttccaccgct tttcagtaat
     2521 ctggttacag ctcaagcgca aaattgctag ataatttttt tcttttattg gcaaaatctg
     2581 ctcctctcta aatagcaatt caggaaacaa ttcaaagaat agtgttcgac tgtctcctaa
     2641 aatctcgatt tctgccggat cctttctttg cttcaataat tggatgaaca tctcagaaaa
     2701 ggactgctct ccatcatctg caaaaaaatg cggaaaatct ttcaaaaagc aaaacagcag
     2761 atatcttact tttctttctg aaccaaagat ctgtgctttt ttataagaaa aagaaaggtg
     2821 gtactcgcca aaccactttc tcaatcgctc ttgcgtagaa cgatcgtact tctgctcgta
     2881 aggttcattc aatagcagat ttttcagtaa acaaaacggg ggtgattgga tcagaaaaac
     2941 aaacaagaga taaggttcgc tatatccttg atcataaact aactgaacgg ttttcttatg
     3001 aagaatcagc tgaatgggac agtcgctttc cgccaaacgt ttcagatgcc gatccactgt
     3061 tttaacatcg attttcagtt cttcagctaa atcagctttt cgccagatca aatctttcat
     3121 tagattgatt agtgaaaata aattctgtgc attttttgat aatagttttt gccatctcat
     3181 cgatcatttc tcacctgatt tctaaacata ttgtttgatc aaaatgggtt cactcctaaa
     3241 aa
//
LOCUS       DACCZY010000185         3197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCZY010000185 DACCZY010000000
VERSION     DACCZY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.193"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..398
                     /locus_tag="IVZ67_002745"
     CDS             <1..398
                     /locus_tag="IVZ67_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HAP8166762.1"
                     /translation="VCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKML
                     EKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLR
                     YRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            370..1086
                     /locus_tag="IVZ67_002746"
     CDS             370..1086
                     /locus_tag="IVZ67_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8166763.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            1083..2201
                     /locus_tag="IVZ67_002747"
     CDS             1083..2201
                     /locus_tag="IVZ67_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8166764.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2194..2811
                     /locus_tag="IVZ67_002748"
     CDS             2194..2811
                     /locus_tag="IVZ67_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8166765.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            2938..>3197
                     /locus_tag="IVZ67_002749"
     CDS             2938..>3197
                     /locus_tag="IVZ67_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8166766.1"
                     /translation="MCRVLNILFQYEKIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPID"
BASE COUNT         1234 a          391 c          610 g          962 t
ORIGIN      
        1 atgtttgctt aaactttgat tcgacaccag taagtattgt tcctagaaaa agaatagcaa
       61 ctagaggtgg ggaaatactg aagggtcaga ttattaaaga tagagaacta attgagacta
      121 cgaaaaagat gttggaaaaa ttaaatgcaa tcggacatat tactattcaa tgtataaaaa
      181 acgaaaaggg aatattttat ttagaaataa atgcaagata cggtggagga gcccccatta
      241 gcattaaatc aggagctaat tcccctgaat atattataaa gatgatagat tcaaaaaaaa
      301 atttgagata cagagaaaat tatttagata aaatgatagc actgagatat gatcaagcga
      361 tttatttaga tgaaaaagga aatgtatata atgattaaag cggtgatctt tgatttagat
      421 gacactcttg tgtctgaata tgattatgta aaaagtggat acaaatgtgt agctagaaga
      481 ttagcagtgc aaaaaatttt cccggctact tttgacgaaa tatatagagt ccttttagat
      541 ctttttgctg aagatagtaa aaatgtattt aatcgtctat atgactattt tgaaattcct
      601 tatagtaaaa aagaaattaa agaactggta aatgtatatc gagaacacaa accagacata
      661 aattttttag aaaatgcaga tactttatta acggaattaa gaagtagagg atacaagctg
      721 gggattatta cagatgggta tgctataacc caacgaaaca aattggatgc tttgaaagta
      781 gactatttgg tagattatat ttgtattact gacgaattag gccgtgaatt gtggaaacca
      841 caccctatct catatctttt attgttagaa aagtttaatt taaaggcaca agagtgtata
      901 tatattggcg ataatgagtt gaaagatttt ataactgcca aatcaatagg aatgcaaaca
      961 attaaaatag aaagagtaaa tggtatttac agagaaagca gtttgaagtt caaaagattt
     1021 taccaagctg ataaaaaaat acgttcgtta tgggagataa aaaatttttt gggagtgatt
     1081 tcatgaaaag taaaagaatt ctattagctt ctccacatat gagtgaagaa ggatatgaaa
     1141 aagagtatgt aaaagaagca tttgatacaa attgggttgc tcctttaggt gcgaatgtga
     1201 atggatttga gaaagaatta gcagaatacg tgggagctag tcatgctgcc gcattatctt
     1261 ctggaacagc agctatacat ttagctttga aagcggctgg ggtcggtaaa gatgacatag
     1321 tgatttgtca gtccttgacg ttctcagcta ctgctaatcc aattctctat caaggagcta
     1381 tacctgtatt tgttgatagt gattacgaaa cttggaatat gaaacctgaa catttgcgaa
     1441 cagcactaga aaaatatcct aacacaaagg ctgtattagt agtacatttg tacggtttat
     1501 cagctaattt agacgaaatt actaaaatat gcaaagaata cgatgttact ctaattgaag
     1561 atgctgcaga atcattagga acaacatata aaggaaaata tactggtaca tttggcgact
     1621 atggaattta ttcttttaat ggaaataaga taatcacaac ttcaggtggc ggaatgcttg
     1681 tatccaatga caaagaaaag gtggaaaaag tgagattttg ggctacgcaa tctcgtgaga
     1741 aggcacgcca ttatcaacac aatgaaattg gctataatta tcgaatgagc aatatcgttg
     1801 ctggtatcgg tcgaggacaa ttgaaagtat taaatgaaag aattgagaaa aaacgatata
     1861 ttcatagttt ttataaaaaa gaattgtcta aattgtcaaa tatacaattc atgccagaaa
     1921 ataattggaa tgaaccgaat tattggttaa gtgtcataac aataaaggga ataaatgcga
     1981 atcatcttat tgatgagtta gacaaaaata atatagaagc ccgcccagta tggaaaccta
     2041 tgcatttaca acctgttttt aaaaattatg attatattgg cgatgatgta gcagaaactt
     2101 tatttgactt aggagtatgt ttaccatcag atacaaagat gacagatgaa gatttaaaat
     2161 atgtagtatc tacgattaaa agagtgatca atgatgcgta aaaaaacttt ctatcagctt
     2221 tttgggaaaa gaattttaga tatcctattg tcaggaattg ctctcatcgt tttatcgccg
     2281 ataatcttaa ttgtaggttt tttagtaaga attaaacttg gttctccaat tatcttcaaa
     2341 caagaacgac caggaaaaag tgaaaaaatt ttttccatgt ataaatttcg tacaatgact
     2401 gacgaaagag accacaatgg agaatattta cctgatgaaa ttcgactaac taaatttggg
     2461 aaaatgctta gagcaacaag tctggatgag ctacccgaac tttggaatat tttgaaaggt
     2521 gatatgtcga tcgtaggacc tcgtccatta ttagtggagt atttgccact atatagtgag
     2581 aagcaacgga aaagacacaa cgtaagacca ggattaactg gactggctca ggtcaatgga
     2641 agaaatgcta ttagttggga agaaaagttt gatttagatg tttattatgt tgataaaatt
     2701 agtttcttta atgatcttat tattataatt cagacatgta aaaaagtaat caagaaagag
     2761 aatattaata caatcaataa caatatccca gaaaaattta aagggagtta aatacagcag
     2821 cttaatgcaa gttttgtgac ttgcattata tttaggtata aatcaaagcg agtatattag
     2881 aattatataa catatgttaa aaaaacgtat agctaataaa agtatgattt taaaaatttg
     2941 tgtagagttt taaatatact gtttcaatac gagaaaatta taaaaaagta taaggaaaag
     3001 agagttatgg caaagtatta taataaaaaa attcttataa tggcaaacaa cgatgatggt
     3061 ctttaccgtt ttcgaggaat gctcataaaa aacttagtag atcaaggtaa tgaagttatt
     3121 gtatctcttc caaagggcaa gaatattcct aaattaattg aacttggatg tcaaattatt
     3181 gaaactccaa tagatag
//
LOCUS       DACCZY010000186         2999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCZY010000186 DACCZY010000000
VERSION     DACCZY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.194"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(93..635)
                     /locus_tag="IVZ67_002750"
     CDS             complement(93..635)
                     /locus_tag="IVZ67_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8166767.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="IVZ67_002751"
     CDS             770..1159
                     /locus_tag="IVZ67_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166768.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="IVZ67_002752"
     CDS             1163..1927
                     /locus_tag="IVZ67_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8166769.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2056..>2999
                     /locus_tag="IVZ67_002753"
     CDS             2056..>2999
                     /locus_tag="IVZ67_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166770.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETT"
BASE COUNT          964 a          501 c          616 g          918 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacaa ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaa
//
LOCUS       DACCZY010000187         2980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCZY010000187 DACCZY010000000
VERSION     DACCZY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.195"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(159..1469)
                     /locus_tag="IVZ67_002754"
     CDS             complement(159..1469)
                     /locus_tag="IVZ67_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8166771.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IVZ67_002755"
     CDS             complement(1481..2899)
                     /locus_tag="IVZ67_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166772.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          931 a          618 c          464 g          967 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc
//
LOCUS       DACCZY010000188         2896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCZY010000188 DACCZY010000000
VERSION     DACCZY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.196"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            223..870
                     /locus_tag="IVZ67_002756"
     CDS             223..870
                     /locus_tag="IVZ67_002756"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8166773.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            1153..1226
                     /locus_tag="IVZ67_002757"
     tRNA            1153..1226
                     /locus_tag="IVZ67_002757"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1187..1189,aa:Ile,seq:cat)
     gene            1772..2338
                     /locus_tag="IVZ67_002758"
                     /pseudo
     CDS             1772..2338
                     /locus_tag="IVZ67_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            2542..2896
                     /locus_tag="IVZ67_002759"
                     /pseudo
     CDS             2542..2896
                     /locus_tag="IVZ67_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1058 a          434 c          527 g          877 t
ORIGIN      
        1 tgcaaatttg tccataactt attgattatc ttgtaaaaat gcggtaattt aatagttagt
       61 gagaagaggt gcttaattat agttaaaaaa gaggaaatag aagcagcatg taaccaagca
      121 aatgttaggt ttattggaga agctttatct ggatatccta agtataaata cttacacaac
      181 tggttacaaa gtaaaagttt ttatcttaat aaagaaagat gcatgcctaa taaaaattat
      241 agggtttata aaagaggaac tattgtatat gttgattttg gggtgaacgt gggttatgag
      301 ttatctggta atcattttgc tattgtttta aataataaag ataattcgaa aaatggtgtt
      361 gtatgtgtag taccaatctc ttcaaaaaag aaaagtaatt atgtatctgt aggaaaaata
      421 ttagaagttg ctagtattaa acaatttata agccaagctg ataaattaaa agagaaactt
      481 aggataatta ttttactttc attaaataga ggcatcattg atgaaagcga tttgaatagt
      541 tttattaaag atattgaagc taaaggtgaa aagctttcag tggaagaagt agaaaaacga
      601 gctgtaaaac ataatttggt ttgcgagaca agggaacaag taattaaaga aataaaatca
      661 ttatctgaag atatggtaaa atatcagaag gtgtttaatt gctacaagaa atatacacaa
      721 gaatcctatg taatgccact taatatccaa acgattagta aaaatagaat tagaagaatt
      781 aacaagtttg atccttctgg aaatatgaag gcacctggaa atgtcctcaa tgaagtagat
      841 gaaagtttaa aaaaatattt tacaaagtaa acgatatttt tgttgacatt tttctgttcg
      901 ttagttacca tgtagttata gaaggacgta agcgcctatt tttaatgtac acatcgggac
      961 gtatgcgccc acatagaaac atagttgaga gacatctata tggtgtctct ttttttatta
     1021 atatgcaaaa aagtctagtt ttttcaataa tatttatgta ataataaatt agccaccaca
     1081 acaaagaatg aaacccatta ttatctagtc tatgtccatt ctttttgttt gcagtagttg
     1141 tgatggcttt ccgtactctt agctcagttg gttagagcag acggctcata accgtccggt
     1201 cgtaggctcg agtcctacag ggtacatact ctaagtgatt taaattgcga ataaaatata
     1261 aaaatgactc cccttttgac tcccccaagc agtatatgga tgtctctaaa agttgttttt
     1321 ttagataata aaaagctgat aataagcgaa aaacacttaa aatcagcttt aaaccctatt
     1381 ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta
     1441 tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac
     1501 aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat gcaagctttt
     1561 gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt
     1621 tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta
     1681 gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta agataaaagt
     1741 gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa
     1801 agaccattat tttggaagga attttgatta tgagatgtct tataatgaag tggtcactat
     1861 tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata aacattatgc
     1921 aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg ctacaaatga
     1981 aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata aagaattaca
     2041 agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag ggcaatgctc
     2101 aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata tcgattttag
     2161 tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa aatcgatagt
     2221 aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg tgttgacaaa
     2281 taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc tatcaaatgg
     2341 aacgagagta taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag gaagtccctt
     2401 ctgtagaata aagttaacga caaccaattc acagaaaaga ggacttccct atgaatgatt
     2461 ttactactga atcggctaca ctagactaga cagaaaaaat aaggtgtgta gaataataaa
     2521 aaacacactg gaggattttt catgccaaga cgtcaacgaa gaacctattc aaaagaattc
     2581 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
     2641 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
     2701 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
     2761 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
     2821 aaagacaagt aattgatgcc aacaagcata aatattccat atcagcgatg tgcaaaattc
     2881 taaatatttc tcgtca
//
LOCUS       DACCZY010000189         2873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCZY010000189 DACCZY010000000
VERSION     DACCZY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.197"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            45..1523
                     /locus_tag="IVZ67_002760"
     CDS             45..1523
                     /locus_tag="IVZ67_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8166774.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1591..2682)
                     /locus_tag="IVZ67_002761"
     CDS             complement(1591..2682)
                     /locus_tag="IVZ67_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8166775.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         1006 a          516 c          455 g          896 t
ORIGIN      
        1 aaaaaattgt ttgttattat tagcaacaaa aaaaggagtg catcatgaaa aagttatctc
       61 aaaaacgttt agctcaaatc attattgatt ctagaaaatc tctgaaaatg acacaacaac
      121 aacttgcagt agctactggt atcaaccgtg ccttaattag tcgattagaa tctctcgatt
      181 ttatcccgtc aatacaacaa ttagaatcat tagcagaagt tctaaatttt gaaccaacag
      241 atctatttat aaatgaatca aataaatcaa acagaacaaa acatcagtcg aagaatattg
      301 ctgtagccgg tactggttac gtaggtcttt ccattgctac tcttcttgcc cagcacaatc
      361 aggtaacagc agtagacatt cttcctgaaa aagtaaaaca aattaacaat aagaaatctc
      421 caatacaaga cgattacatt gaaaaatatc tagctgaaaa aaatcttaat ttaattgcaa
      481 cgactgatgg tgcagccgcc tatagagaag ccgattttgt tgtcattgct gctccaacaa
      541 attatgatag taaacaaaat tattttgata cttctgctgt agagtcagta atcaaattgg
      601 tgttagaagt caatccaaag gcaataatgg tcatcaagtc gacggtaccc gtaggctata
      661 ccgaaagtat tcgtaaaaaa tacaatacta aaaatattct attcagccca gaatttctca
      721 gagaatctaa ggcattatat gacaatctat atccttcgcg aattattgtc ggcacggacc
      781 aaactgacaa aactttacga gaagcctctc acacttttgc agatttactc caagaaggag
      841 caatcaaaaa agatatcgaa actcttttca tgggattcac agaagctgaa gcagttaaat
      901 tattcgctaa tacttacctt gcacttcgag tatcttactt caatgagctg gatacttatg
      961 cagaaatgaa aggactaaat acacaagaaa tcattcacgg ggtttgctta gatccaagaa
     1021 ttggcacaca ttataataat ccatcttttg gatatggagg ctattgttta ccgaaagata
     1081 caaaacaact tctcactaac tataacgatg ttcctcagaa tatgataagt gcaattgtcg
     1141 atagtaatcg tacaagaaaa gactttatag cagatcgagt gttagaactt gctggcacgt
     1201 atgaggctaa cagttcgtgg aatgcagaaa aagaaaaaga ggtagtcata ggtgtatacc
     1261 gtcttacaat gaagagtaat tcagacaact tccgtcagtc atctattcaa ggagttatga
     1321 agcgaattaa agcaaagggg gcaactattg taatttatga accaacactt gaaaataaca
     1381 ctacattttt tggttcaaaa gtaataaata atttagatga ttttaaaaat atcagtcaca
     1441 gtattattgc taatcgttac gatagtaatc ttgatgatgt aaaagaaaaa gtatatacca
     1501 gagatttatt tcaacgagat taaaaaataa cacctcagat ttgtagtgtc tgaggtgttt
     1561 ctataaactt ttataagtaa aaaaatacaa ttatacttca taaaaagact tataccactg
     1621 cgcaaattta cgaagcccgt cacgcaaact tgtacttggt ttaaattcaa aatctcgttc
     1681 taacgcacta gtatctgcat aagtaactgg cacatctcct ggttgcattg gaactagttc
     1741 ggtatgagat tcaaagtcgt aatcttctgg tagcacccct gctgcaatta gctcttgttg
     1801 tagaatatct acaaaatcta gtaggttttc cggcgtattg ttaccaatat tataaatatg
     1861 atatggtggg attggtagcc catcttcccc atcaattttc tctggagcgc actgcataac
     1921 cctctttact ccttcaacaa tatcatctat ataggtaaag tcacgcttgc aattcccata
     1981 attgaatatt tggatattat caccagcacg aagtttatca gtaaaaccaa aataagccat
     2041 atctggacgc ccagcaggac catatactgt aaagaaacgt aaccctgtag acggaatatt
     2101 gtaaagttta gaatatgcat gtgccattga ttcattactt ttcttagttg ctgcatacaa
     2161 cgatacttga ttgtctactt tatcctctgt agaataagga attttcttat tagtaccata
     2221 aacggaagac gatgaggcat acactaaatg ttccacacca gattctccat tatcatatga
     2281 atgacgacaa gcttcaagaa tattatagaa tcctattatg ttagattcga tatacatatc
     2341 tggattagta attgaataac ggactcctgc ctgagctgct aaattcacca cgatacttgg
     2401 tttataatta tcaaagatga aatcaattgt tgctttatca gcaatagaac ctttgataaa
     2461 attccaagta gatcctggat ggttctttgc ctccgtttct atctctgata atctatagtt
     2521 cttaatagag acatcatagt aatcattcat attgtcaatg ccaatgattt ttattgatga
     2581 ctgagtacgt aataactcaa gaaccaaatt tgatccaata aaaccggctg cgccagttat
     2641 taatattgtt ctatttgata aggacgtgtt taattccagc attttttagc actcactttc
     2701 tttttttctt atgaatacat atttgttata tattaaaaaa tcttatttag acttatctat
     2761 tgtcactttt atcattctaa tctttgcacg tagtagaata attatacaga taggaaattg
     2821 ctaagcaacc cacttctttc tattatctat taacttattc taaaacgaat aga
//
LOCUS       DACCZY010000190         2838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCZY010000190 DACCZY010000000
VERSION     DACCZY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.198"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            15..890
                     /locus_tag="IVZ67_002762"
     CDS             15..890
                     /locus_tag="IVZ67_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166776.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1035..1187)
                     /locus_tag="IVZ67_002763"
                     /pseudo
     CDS             complement(1035..1187)
                     /locus_tag="IVZ67_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1455..2786)
                     /locus_tag="IVZ67_002764"
     CDS             complement(1455..2786)
                     /locus_tag="IVZ67_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8166777.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          989 a          523 c          448 g          878 t
ORIGIN      
        1 gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg
       61 gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt
      121 ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca
      181 acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg
      241 tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct
      301 cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta
      361 tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat gcaataaatt
      421 attcagataa attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa
      481 acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat
      541 cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta
      601 ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact
      661 gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt
      721 tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcca
      781 taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag
      841 ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
      901 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
      961 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
     1021 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta gattttccgg
     1081 cattgttgat agattttatg atacttttct acattttgta agagaggttc cactgcatct
     1141 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc taatctttaa
     1201 agataaatat taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa
     1261 cctatctttg catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat
     1321 aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata
     1381 tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt
     1441 tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt
     1501 cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct
     1561 gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga
     1621 atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa
     1681 gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat
     1741 ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag
     1801 tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt
     1861 cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt
     1921 tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct
     1981 aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata
     2041 gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact
     2101 atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat
     2161 aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca
     2221 gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata
     2281 aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta
     2341 taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac
     2401 atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata
     2461 tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac aactaataat
     2521 gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat
     2581 gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata
     2641 agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa
     2701 gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca
     2761 atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta
     2821 ctttttccac ccaaataa
//
LOCUS       DACCZY010000191         2750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCZY010000191 DACCZY010000000
VERSION     DACCZY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.200"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            58..480
                     /locus_tag="IVZ67_002765"
     CDS             58..480
                     /locus_tag="IVZ67_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166778.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            497..919
                     /locus_tag="IVZ67_002766"
     CDS             497..919
                     /locus_tag="IVZ67_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8166779.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            948..1148
                     /locus_tag="IVZ67_002767"
     CDS             948..1148
                     /locus_tag="IVZ67_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166780.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1235..2123
                     /locus_tag="IVZ67_002768"
                     /pseudo
     CDS             1235..2123
                     /locus_tag="IVZ67_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            2261..>2750
                     /locus_tag="IVZ67_002769"
     CDS             2261..>2750
                     /locus_tag="IVZ67_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166781.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITS"
BASE COUNT         1091 a          444 c          494 g          721 t
ORIGIN      
        1 aagctatttt tactcttttt cgtaaattat tttttcgtgt tggaaaggaa cgttggaatg
       61 acaacatttg aaagaataaa agaactagcg aaaaacaaga ataaaaacct tcaacaagtc
      121 gctaaagatt tgaagtacgg agagaattac ttttatagtt taaacgcagg aaaacagcca
      181 actgccgaaa agttacaaga aattgctgac tacttcgacg tatctgtaga ttatttgcta
      241 ggacgtactg agaaaaaaag atactatgat ttaacagaaa aagacgagcg tgatatccaa
      301 aaagaattgc agtcattaat cgacgattta tctaatgctg atggaatggc tttttcgaag
      361 aaagataatg aaatgagtga agcgacacgt gaagctctca ttatttcttt agagaatgct
      421 ttgcgaatag ctaaaataga agctaagaaa aaatacacac ctaaaaaata tcgcgattaa
      481 aaggagtacg ttgtttatgt atttacctca aattgataac aaaattaata agctggtaaa
      541 actatatcaa acacgcaatc cttttagaat tgcacgagaa ttgaagatac tagttttaga
      601 agaagatttg ggagaaattt atggttacta tagtaaattc aaacagataa aaatgatcca
      661 cataaattct agtttagatg agatgggaaa atggataact tgtagtcatg aattaggcca
      721 ctgtgttttg catccgaatg aaaacacacc tatgttgtct aaaaaaacgc tagtatctga
      781 attaaaaatt gaaaaagaag caaattattt cgcaactcat ctgactattg atccaacttt
      841 agacggcttt gatcatatga caaaatatga aaagctcgtc tgctttggat taccagatga
      901 attcgatcgt ttcttgtgaa ttataataca acgtaaggag gatatagatg aaaataggaa
      961 tgagaaaacc aagtataaaa aaatccataa gcgctagaac cactggaaaa gcaaaaagaa
     1021 aagtaaaaaa agcagttatt cctggttatg ggaaaaaagg tagcggctgg attaaagacc
     1081 caaaaaaggc tgcttacaat aaagtataca agaaaactac attcagtttt tgggatttat
     1141 ttaaataaaa aaagcccgtg cagcaacacg gactcgaaac cttatttcta agattcccca
     1201 ataaaaatca tatcatagaa atgaggaata aaaaatgaaa aaagtaataa caagtttatt
     1261 agtttttgct tctctgttac ttttcactgc ttgtggtaaa aaagtaacta cagaagattt
     1321 aaaagctaat gattggttga ttgaagcaac caaagatgac gaacctaata tgatcgtatc
     1381 attttctgat cacgtcgtat cttttagtgt tgacacaagt tctatgaaat caacttcaag
     1441 taacgattgg gaggctatgg gagaagaatt tgctaaaagc ttagtcgacc aaatgaatta
     1501 caaatttgag tatacacttg aaggcgatgt tatgacttgg aaagatgata aagacaaaga
     1561 taatgatgcc aaatatactg tcagcaaaga agataaaaat attattttaa ctccagaaaa
     1621 gcctaacaaa tctgatgata atgagaaatt agttttgaaa ccctacaaaa aggaaaaaaa
     1681 ctaaagaatc tagaacaact actagctcaa caagtagcaa cgaacaatca tcttcgacta
     1741 caacagaaag tactattgca tcttctagta ctgtagaaca agctgctatt gcgcagaata
     1801 cgcctcctga aactagtagt acttactctg ctgaaagtgt agaaaactat gcagaagcta
     1861 ctcctagttc aagtgttgaa caagtttcac ccactcccag ttcaacggag caatcagtac
     1921 cacaagatta tgtaccagct aatcaagctc aaccaacggc accttcttca tctgaaaccg
     1981 aaaccttgac tttgcaacct ggtgaaggga ccaaacaaat agctgaacga gctggaatta
     2041 cggtagatca actgtttgca ttaaacggta tggatccaaa caactacatg ctttatccag
     2101 gacaagaact aagagtgaaa taaaaaaagc acatttgccg cccgaccaag aacgcaaatg
     2161 tgcaaagagc tacggtaaag taggtctatt tgttgtaccc tattttacca tctcgaaatc
     2221 cgctgtacaa ccgaacaaac gtacgaaagg aaagataaag atggcaaaaa aagaaattga
     2281 taaacgaatt aaaaagcact taacaaaaaa aggcgaagaa aaatatcagt ttagtctgta
     2341 cttaggagtt gatcctctaa caggaaaaaa aagaaaaaca acgagacgag gttttagtac
     2401 gccattagcc gctgagagag ctttaaaaag attggaggca gatatacagg aaaaaggact
     2461 gcaagcttct acagctcaaa agagcaagaa attcgaatat gtttacggct tatggtttga
     2521 aaattataaa aagacagtta aagaaagcac ttggtcctcc accaaacaaa tttttgacac
     2581 acacatctta aaagtgtttg gagataagtt cattgacaaa atagacgttt ttttctgtca
     2641 agaagcagta aacacttggt ccgatagcca tcctaaaatt tttaagaaaa taaaaaatta
     2701 cacatctaat gtttttgatt acgctgcttc tttacaaatt ataactagca
//
LOCUS       DACCZY010000192         2743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCZY010000192 DACCZY010000000
VERSION     DACCZY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.201"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..1350
                     /locus_tag="IVZ67_002770"
     CDS             <1..1350
                     /locus_tag="IVZ67_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8166782.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2743
                     /locus_tag="IVZ67_002771"
     CDS             1484..>2743
                     /locus_tag="IVZ67_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8166783.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKM"
BASE COUNT         1079 a          375 c          539 g          750 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atg
//
LOCUS       DACCZY010000193         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCZY010000193 DACCZY010000000
VERSION     DACCZY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.202"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVZ67_002772"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVZ67_002772"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8166784.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1073 a          425 c          557 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaaa aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DACCZY010000194         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCZY010000194 DACCZY010000000
VERSION     DACCZY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.203"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(75..1874)
                     /locus_tag="IVZ67_002773"
     CDS             complement(75..1874)
                     /locus_tag="IVZ67_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8166785.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1998..2594)
                     /locus_tag="IVZ67_002774"
     CDS             complement(1998..2594)
                     /locus_tag="IVZ67_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8166786.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          958 a          516 c          478 g          773 t
ORIGIN      
        1 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
       61 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      121 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      181 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      241 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      301 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      361 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      421 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
      481 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
      541 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
      601 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
      661 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
      721 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
      781 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
      841 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
      901 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
      961 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1021 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1081 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1141 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1201 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1261 gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1321 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1381 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1441 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     1501 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     1561 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     1621 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     1681 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     1741 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     1801 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     1861 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     1921 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     1981 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2041 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2101 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2161 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2221 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2281 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2341 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2401 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     2461 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     2521 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     2581 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     2641 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     2701 tcataagtat aaaagattga tctca
//
LOCUS       DACCZY010000195         2679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCZY010000195 DACCZY010000000
VERSION     DACCZY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.204"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            20..466
                     /locus_tag="IVZ67_002775"
     CDS             20..466
                     /locus_tag="IVZ67_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8166787.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            739..2091
                     /locus_tag="IVZ67_002776"
     CDS             739..2091
                     /locus_tag="IVZ67_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8166788.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2262..2549
                     /locus_tag="IVZ67_002777"
     CDS             2262..2549
                     /locus_tag="IVZ67_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166789.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          938 a          446 c          534 g          761 t
ORIGIN      
        1 aacaacgtgg ggtgtttaca tggaaaataa gttagtcgaa gaggttatcg aaaagttaaa
       61 aaaagagaac attcgaatca cacctcaacg gtacgcggtt ttagaatatt tgattgaaca
      121 tcattcccat ccaacggctg atgagattta taaagcactt gaacatcggt ttccaaatat
      181 gagtgttgcg actgtttaca ataaccttcg cttatttact tcgattggat ttgttcaaga
      241 aatgaagtac ggcgatgctt ctagccgatt tgatttcagt tcaaagcgtc attatcatgt
      301 aatttgtgaa aactgcggta agattgttga ttttcattac ccagggcttg aagatataga
      361 aatggctgca agcagactga ctgattttga gattaatgaa catcggttgg aattgtatgg
      421 actctgtccg gattgtaaag agtcagcaaa aaaggcgcaa atgtaaaaac agtttctaca
      481 agtgttatac ctaaaaaaag ctagctgtgt aaaaaaaacg ctagcttttt tatttttttg
      541 tcttttattc acaatcttag atataaaagt gaatattatt aaaataagaa tcttgtagat
      601 attgggttct tatgcttgaa aaggaatgaa actacaaaca aacctttgtg aattcacaag
      661 cattaaactg ttgtatccgt tttatttctg ttatagtagt tttgtcgaag gacaaaatgt
      721 taatcggagg gaccataaat gaaagtagta gtaattggct gtactcatgc aggtactgca
      781 gctgtaaaaa gtattttaaa aaatcatcca gaagcagaag tgacagtata tgaaagaaat
      841 gacaatattt ctttcttatc ttgcggtatc gcactttatg tcggcggagt agtaaaggac
      901 ccagcaggct tgttctactc aaatcctgaa gaattatctt cattaggagc aacagttaaa
      961 atggaacacg atgtaacaga cgtcgataca gaaaataaaa cagtaacggc aaaagatctg
     1021 aaaacaggag aaacagaaac ggtttcttat gataaattag tgatgacaac tggttcatgg
     1081 cctattatcc caccgattag tggaatcgat gcgaaaaatg tactcctatg caaaaactac
     1141 aatcaagcaa atgaaattat tgcacaagcg aaagaagcaa aacgtgtagt aattgttggt
     1201 ggtggatata tcggtattga attagtagaa gcttttgtcg aatctggaaa acaagtgaca
     1261 ttgattgatg gattagatcg catcttgaat aaatatctag ataaaccatt cacagatatc
     1321 ttagaaaaag aattgacaga ccgcggtgtt actcttgcgc taggcgaaaa tgttactgaa
     1381 tttatcacag atgaagaagg aaaagtgaaa caagtagcta caccaacaga tacatttgat
     1441 acagatatgg tcattttatg tgtgggcttc cgtccaaata caaaactgat cgaaggaaaa
     1501 gtagatacac tgccaaacgg ggcaatcaaa gtgaatgaat acatgcagac aagtaatcca
     1561 gacatttttg cagctggaga ctctgcagta gtcaactaca atccaagtgg cacgcaaaac
     1621 tatattcctt tggcaacaaa tgccgttcgt caaggtttgc ttgtgggaaa taacttgaca
     1681 gatcataaaa tggcgtatcg tggtactcaa ggaacttcag gactttacct atttggctgg
     1741 acgatcggtt cgacaggtgt gaccaaagaa agcgcaactt tgaacgatct agaagtccaa
     1801 gcgactgtat ttgaagataa ttatcgtcca gagttcatgc caactactga aaaagtaatg
     1861 atggagctag tctatgaaaa aggaacgaat cgtatcgtag gtgctcagtt catgtccaaa
     1921 tacgacatta cacaatcagc aaataccatg tctttagctg ttcaaaataa aatgactgta
     1981 gaagatctcg cactttcaga tttcttcttc caaccacatt ttgaccgccc atggaattac
     2041 ttgaacctac ttgcacaagc agcgctaggc gaaatcgaaa ctgcacaata aagaaaaagg
     2101 gttgtgacac aattttgtca cgaccctctg tctttttttg tgaaaagtac aattagttgt
     2161 ctttcttcga attttttgtc acaataagca gcaaagataa agctgttaca taaaagaaca
     2221 agcagcttta tttgattttg aaaagaaagg tgagaaagaa gatgaataaa gaatattttg
     2281 tccaacaaga tgcattttta gaagattata aaagtgcttc gaagaaatta acgatcaata
     2341 ttgatgagtt ggcagatcaa gtactctctc agttgaatga aacgcaagag gaatattaca
     2401 cgatgccagc aaatgacgcc aaagataatg tttctcatcg ttttcctttc agtagacgca
     2461 tctactcaga tgaaaatgac ggcacattga atacagagta cacatatgaa ggtcagccat
     2521 atgtgctaga aactgatgat gatctttaaa gacagttttt gaaaaggccg ctcatcctta
     2581 agtgatgaca aaaaatcatc acaattaccg atgactgtct ctttactatc ttttaatcag
     2641 aaaaaggggt tatgacaagc tttgtcaacc ccttttttt
//
LOCUS       DACCZY010000196         2642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCZY010000196 DACCZY010000000
VERSION     DACCZY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.205"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            278..1063
                     /locus_tag="IVZ67_002778"
     CDS             278..1063
                     /locus_tag="IVZ67_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8166790.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="IVZ67_002779"
     CDS             1076..1927
                     /locus_tag="IVZ67_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8166791.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="IVZ67_002780"
     CDS             complement(2051..2503)
                     /locus_tag="IVZ67_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166792.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT          828 a          435 c          562 g          817 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 tt
//
LOCUS       DACCZY010000197         2627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCZY010000197 DACCZY010000000
VERSION     DACCZY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.206"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(96..1139)
                     /locus_tag="IVZ67_002781"
     CDS             complement(96..1139)
                     /locus_tag="IVZ67_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8166793.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1516..2481)
                     /locus_tag="IVZ67_002782"
     CDS             complement(1516..2481)
                     /locus_tag="IVZ67_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8166794.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          936 a          482 c          499 g          710 t
ORIGIN      
        1 attgttgttg ttagaaggat atctgcaaaa ttaaaaacag ttgtgattcg gacattgttc
       61 catgcgagaa tcacaactgt tttttcatgt tcattctaaa ctttgatcag ccaaataaat
      121 agtaaagcca tcactgtttg gagaagcccg acagctaatc cataagataa gaagcgtgta
      181 ccttctttca tgaattttgc aaagtctaaa cgcagaccaa ttgccgctaa agcagtgatc
      241 tcaaaccagc tgctgataaa atgtgccgat tggtcaataa ctacaggtaa agaaaacaag
      301 ctgttgacca aacaaaagat cacaaaccct aatacatacc aaggaagcgg taattttcgt
      361 tttttctgcg tatcagttgg cgtttcaaac agagctggtt gtttcttgtg catgaatttt
      421 tcaaacagat agacaacaac aacgagcatc ataatacgta gaatcttaaa tagcatggcg
      481 aattcgacaa cagaattgtc aagtaagctt gccccagcga ctacttgacc aacagattgg
      541 agtgtaccac ccaaaagtgc acttttttca atgagatttc caccaaacaa ctgtaatcca
      601 agaaaaggga gagtcaacat catcacagta cccagtagat tgactagtgt gatgatctgt
      661 cctttttctt cctctttcgc atggatggaa ggggcgattg aagcaatagc agaagaacca
      721 caaacagcgt ttcctccagc catcatcaaa gaagaagtat cgctgaattg cagtttttta
      781 ccaatcaagt aagcaccgat gatagtgact gacatttgca gtaacacaaa aacaattcct
      841 tttacaccaa tttgagcaat cgtttgagct gtgacagttg cacctagtaa aaaaacagag
      901 aattctaata gtttgctttc agcaaatttt gtgccgattg ctagaacagg ctgtttaaaa
      961 aaagtatttc ctaataaaat acccaataaa atagcaatag tcgctccacc taactgtggc
     1021 agataaatag caagaagcct gctgaaggca gcgatggcaa aagaaagcag taaaccgggt
     1081 aagatttcgc ggaccgctga atcctttaca gattttattg gatgtgacaa tgatttcatt
     1141 tgatcggttc ctttcgtcaa taaatactaa aagcatactc gaacatagta gcactaaacc
     1201 agtattttga caaaagaaca agaggtcgtg acaaaagtct tgtacgacct cttgtttcga
     1261 ataaacggtg ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaatttga
     1321 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctt ttacgacctc
     1381 ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag tacaaaaaca
     1441 tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct ttttcacaac
     1501 ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt tgctatgttc
     1561 tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac ttgcaaacag
     1621 acattccgca acttttaaga aatccaatac gctactatag atacctaaac tgttgaagta
     1681 atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa aggctgcata
     1741 gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa cgaaatacaa
     1801 tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag caaagttttt
     1861 aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca gcggacgtgt
     1921 cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca ataagaacgc
     1981 atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga agttcccggc
     2041 agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat aaattgcttc
     2101 aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa ggatcacttg
     2161 gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt catatgaaac
     2221 aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg aggcgatgat
     2281 tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa caaaaatgaa
     2341 tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat ggtgatagtc
     2401 tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg ggatcggaat
     2461 aattttcaaa aagttcttca tatataatat tctccttttt atttaataga ttttagtacc
     2521 aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca agcagacagc
     2581 tgattgtctt catctaagtt actagttgga tcagcaactg ttttgtt
//
LOCUS       DACCZY010000198         2572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCZY010000198 DACCZY010000000
VERSION     DACCZY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.207"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            26..1033
                     /locus_tag="IVZ67_002783"
     CDS             26..1033
                     /locus_tag="IVZ67_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8166795.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1045..2049
                     /gene="neuB"
                     /locus_tag="IVZ67_002784"
     CDS             1045..2049
                     /gene="neuB"
                     /locus_tag="IVZ67_002784"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8166796.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2046..>2572
                     /locus_tag="IVZ67_002785"
     CDS             2046..>2572
                     /locus_tag="IVZ67_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8166797.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPR"
BASE COUNT          939 a          333 c          516 g          784 t
ORIGIN      
        1 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
       61 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      121 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      181 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      241 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      301 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      361 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      421 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      481 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      541 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      601 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      661 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      721 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      781 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      841 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      901 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
      961 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1021 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1081 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1141 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1201 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1261 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1321 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1381 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1441 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1501 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1561 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1621 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1681 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1741 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1801 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1861 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1921 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     1981 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2041 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2101 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2161 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2221 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2281 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2341 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2401 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2461 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2521 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga at
//
LOCUS       DACCZY010000199         2491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCZY010000199 DACCZY010000000
VERSION     DACCZY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.208"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(36..401)
                     /locus_tag="IVZ67_002786"
     CDS             complement(36..401)
                     /locus_tag="IVZ67_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166798.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            609..1091
                     /locus_tag="IVZ67_002787"
     CDS             609..1091
                     /locus_tag="IVZ67_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166799.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1288..2178
                     /gene="pstS"
                     /locus_tag="IVZ67_002788"
     CDS             1288..2178
                     /gene="pstS"
                     /locus_tag="IVZ67_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8166800.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          837 a          417 c          499 g          738 t
ORIGIN      
        1 aaagtagctt ctctttgtac atatatggtt tgtttttatg gatgccgagc ttttttataa
       61 gataaggcta ccgcccataa cgacccaagg atttgtggaa ctaagtaaat acctgcaaga
      121 agcaagatca gctgggtcgg ttttaaagga tgatagaagt aacgccattc agtaaaattg
      181 gcgcaaaata aataggaagt gacagtagat ataatattgg caatgaataa agatataata
      241 cgcctgttcc tttgaaaata atatccagca aaaagagata aaaatatcaa tgtgatgaac
      301 ccgcttacgg aatgataacg aaaatcaagc agtaaggcga aatgcacata aggaacaaaa
      361 gataaaagca acggaaggtt tttccagcaa gtttttatca tctttcatca ccacctaaca
      421 agtgttatta ttttgttagt tttattgtaa attgaatcct gcaataatca attacttttt
      481 cattatttct agattaaatg aataaactct catgccatta tctctaaaaa aaaagttttt
      541 ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag tagtttgggg
      601 gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt tggttttttc
      661 ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg gagatattag
      721 gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg attgttttct
      781 atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac tacaattggc
      841 agctgcttta cttttcttgc ttattagtat ttacatctac gttttctttg ctgcctcgct
      901 tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct tattcctcca
      961 tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt attcttgcaa
     1021 cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta tctataaaaa
     1081 ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt
     1141 ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat gatttttaca
     1201 taacttaact atatttttac agcaaccata cgagggattt acagtcgact gataaacttt
     1261 agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct aggtatgatg
     1321 ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc tgccacacaa
     1381 aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc gacagcatta
     1441 cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc
     1501 tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg agcagttact
     1561 atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc aaaattagtg
     1621 gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac agatgtcaaa
     1681 gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa ctggaaggaa
     1741 gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc
     1801 gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca agaacaagat
     1861 tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag ctatttagct
     1921 ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga accaaaagaa
     1981 gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta cacgaacgga
     2041 aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga aatccaagaa
     2101 ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga acgtgatcac
     2161 gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac tattaaggaa
     2221 aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa tagttcctca
     2281 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttattcgaa
     2341 aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg
     2401 gtgaagacaa atttgaataa aacttagacc gatatgcggc gcgttctgtg cgccgcatat
     2461 ttttttagtt ttcaaagatc agaccttgtt g
//
LOCUS       DACCZY010000200         2490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCZY010000200 DACCZY010000000
VERSION     DACCZY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.209"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVZ67_002789"
     CDS             64..972
                     /locus_tag="IVZ67_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8166801.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="IVZ67_002790"
     CDS             972..1592
                     /locus_tag="IVZ67_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166802.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="IVZ67_002791"
     CDS             1596..2045
                     /locus_tag="IVZ67_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166803.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..>2490
                     /locus_tag="IVZ67_002792"
     CDS             2045..>2490
                     /locus_tag="IVZ67_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166804.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          878 a          376 c          570 g          666 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa
//
LOCUS       DACCZY010000201         2457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCZY010000201 DACCZY010000000
VERSION     DACCZY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.210"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            154..2307
                     /locus_tag="IVZ67_002793"
     CDS             154..2307
                     /locus_tag="IVZ67_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8166805.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          839 a          428 c          544 g          646 t
ORIGIN      
        1 ggatatgtaa ctttaagtga ttgctgtgaa acaaaaagtt gattttatct tgaaattagg
       61 taaagaattt cttgctccgc ccattttttc ttttttatta agaagttttt gggtacaatg
      121 aagcatgaat gtaaataggc ggaaggatag gaaatgatga aagattttat gaaaaaaata
      181 ttaaatactt cttggttaga aaaaataaga aaaggtgcac aagaacctaa gaacaaaacg
      241 tttcgccggt cccatgttcc ttttcgctta aattttcttt ttttcattat atttacgtta
      301 tttgttgcgt tgattgctag attgggttat ttgcagatcg tgaacggcga agagatggag
      361 gcgcgagtga agtctacttc tacgattacg gtccaagaaa gtactcctcg cggaatgatc
      421 tacgactcca caggaaaagc tttagttacg aataaagcga atcaggccat cacatatacg
      481 agaggaacac aagttacggc taaagatttg cttgaaatcg caacgaagct gaactcgctg
      541 atcgatgttc cggtagatga aaatctgaca gatagagata aaaaagattt ttggctggct
      601 gatcccaaac atttgaaaga agccacaaaa agactttcgg caaaagaaaa gcaattaggg
      661 aacagcgaac aatatagtgc gatggtcaat aaagtcacag ctgatgagat taatttaaac
      721 gaggagcaat taaaaattgc taccatcttt aaacggatga atagtgcata tgaattaaac
      781 actgtctata tcaaaaactc tggtgttact gatcaagagt tagctgttgt ggcagaacgc
      841 gcttctgaac ttccaggagt ttcgactggc acagactgga cacgagaata taatgcagca
      901 agttcactga aaagtattct tggcagtgtg acgactgaaa agcaaggact tccagcagat
      961 gaagcagaga aatacctagc aaaaggttat tctcggaatg accgtgtagg acaaagttat
     1021 ttggaaaaac aatatgaaga tgtcctgcaa ggaaccaaaa cccaatatga agtttccctt
     1081 gataatgaag gaaatgtttc gaatcaaaaa gaaatcttct ctggggaaaa aggttctaat
     1141 ttaatgttat ctatgaatgc agaattccaa tcaaaagtag aagaaatttt gaaaaggaat
     1201 tatcaaacat tgatcaacaa cggcaaagca cagtattcgc ctggtgctta tgcagtggct
     1261 atgaatcctc agacaggaga agtgctagca atgactggtt tcagtcatga acaaggttcc
     1321 aaagaaatca ccgagaatgc tttaggcacg attactagcg cctttgatcc agggtctgta
     1381 gtcaaggctg gtactttgac agcaggctgg gcaagtaacg cgatcagcgg aaatcaagtt
     1441 ttgattgatg agccgattcg tctgcaaggt gcttcagaaa aaagctctgt ctttaatcga
     1501 agcggacaag ttgctcttga tgcagtcaaa gcatcggaat tgtcttctaa tacgtatatg
     1561 atcaaagtgg cgttaaagat gttaggacta gactatacac ctggaatggg actacctagt
     1621 ttagatgaag aagctaaagc ttatcagcaa ttgagagact ctttcaaaga gtttggtctt
     1681 ggtacaacga ctgggatcga tttgccgaat gaatctcctg ggatctcacg ttctgttgat
     1741 tatatgaaga agttcaatgc ggataatggc aaagaatggt atgctccagg taactttacc
     1801 gatttggcat ttggacagtt tgacacatat acaccaatcc aattagctca atatgcttca
     1861 acagtcgcta atggcgggaa acgagtacag ccgcacctag tcaaagcgat ctatggcaat
     1921 gacgaaaatg gaaacttggg tgaagtgaag aaagaaattg gcacaacagt agaaaatacg
     1981 gtcaatattt ctgcagaaaa tatggcgatc ctccgtgaag gattccacca agttgtccat
     2041 ggtaccgatc cttacacaac agccaaacca ttagccagtg cgaaaatgga cctttccgca
     2101 aaaacaggga ctgcagaaac ggttgcggaa ggtcatccag atattgcgac agtcaatagt
     2161 aatatcgtgg catacggacc aacagacaat ccggaaattg ccatcagtgt tgtcttgccg
     2221 aacttattag acgaacaaga ccacataaac ttgacgattg ctaaagaaat aatggacgcg
     2281 tattatgata tgttcatggc aaaataaaaa agaggtcgtg aaaaagccgc ttagtttcga
     2341 gtatgaagaa aaattttgtg aaaatagctt ctcacatttt tgcttaaatt tgacttcata
     2401 ccgagaaact ggcttttgaa caccgtttac tcggcaaaaa aggttgtgac aaaaatt
//
LOCUS       DACCZY010000202         2444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCZY010000202 DACCZY010000000
VERSION     DACCZY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.211"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            52..741
                     /locus_tag="IVZ67_002794"
     CDS             52..741
                     /locus_tag="IVZ67_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8166806.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            755..2257
                     /locus_tag="IVZ67_002795"
     CDS             755..2257
                     /locus_tag="IVZ67_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8166807.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2270..>2444
                     /locus_tag="IVZ67_002796"
     CDS             2270..>2444
                     /locus_tag="IVZ67_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8166808.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRV"
BASE COUNT          776 a          336 c          499 g          833 t
ORIGIN      
        1 gtttttggag ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa
       61 aagaggtttt tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt
      121 gaaccatttt acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg
      181 gctggagcag ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt
      241 gtatcattac caatgattgg aattattaaa agagattatc caccagaatc tccattcatt
      301 actgccacta tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt
      361 gattgcactc ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata
      421 aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa
      481 aatgcatata aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa
      541 agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt
      601 tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata
      661 ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga
      721 tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg
      781 acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg
      841 aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt
      901 gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact
      961 aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt
     1021 agctggtgga gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt
     1081 ttcagcaaaa gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac
     1141 tgttgcattt ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt
     1201 tggaggatca agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt
     1261 cttttattta atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc
     1321 ccaaatgggt agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt
     1381 tggactacac cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac
     1441 tgtagctgga tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc
     1501 aaaccacact ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat
     1561 gatgtttgga ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg
     1621 taaaaagaat ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac
     1681 tgaaccagtt gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt
     1741 cttagatgga ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt
     1801 ttcaggtggt ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa
     1861 ttggttaaaa ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac
     1921 attctttatc aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca
     1981 ttctgaaaaa gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga
     2041 agctcttggt ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt
     2101 tgctgttacg aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc
     2161 tgtcttcgaa gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag
     2221 tcaagaaatc aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat
     2281 tttcaaaaag aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat
     2341 cacagaagta aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat
     2401 acctgatggt tcaaaaattt atgctccatt ggctggtcga gtaa
//
LOCUS       DACCZY010000203         2426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCZY010000203 DACCZY010000000
VERSION     DACCZY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.212"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            37..1206
                     /locus_tag="IVZ67_002797"
     CDS             37..1206
                     /locus_tag="IVZ67_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8166809.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1473..2387)
                     /locus_tag="IVZ67_002798"
     CDS             complement(1473..2387)
                     /locus_tag="IVZ67_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166810.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          887 a          392 c          362 g          785 t
ORIGIN      
        1 tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa acatgctaaa
       61 aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct
      121 acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat agttgcccat
      181 gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct cgttgaaaaa
      241 atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa gaaaaataat
      301 cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca accaaatgat
      361 ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct tttgatttta
      421 aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt ctatccacaa
      481 attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc aggcttgcac
      541 cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt tgcgattcag
      601 cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt taccatattg
      661 actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac cgatgatatc
      721 atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat
      781 catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact gacagaatct
      841 gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt ctatgattta
      901 aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt actcttctca
      961 ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag cggagataat
     1021 tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt tagcaaagat
     1081 tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat caaactgcct
     1141 actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa gttggcaaat
     1201 agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg cttaaaaaaa
     1261 gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt tttgctacag
     1321 actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc tgtccaaatt
     1381 attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat ctaaaatagt
     1441 atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct tatttttaat
     1501 tttatgcaat aaaaaagaag aacaattagc gtgattatag aaataaataa tgagatttta
     1561 gaaaaaaatg tgtatgaata cctgattgtt actttattta ttccactttc actttttact
     1621 gtaacaaaag gacctccttg ttttagtact gtagcatttt ttgaattaac agtaaccgtt
     1681 aatcctttat accggaatac aggtaaaact attgttttgc cttttgaaga tttaaacgta
     1741 aatgtaattt catctttatt tggagaaaag ctactataaa taacttgacc atcttctgta
     1801 acttgatggt ttttcaaaag caattgatct tttttatagc ctataggtac ataatcaaaa
     1861 ttctcaaatt cttcaagttt attttccatt tgttcattag aataagttcc ttgaaaattc
     1921 atttctttca ccatgttaac tactgatgaa taatttaaaa ttgaaatagc taatattaat
     1981 aatagtataa aatttcttct ccttgtataa gaaaattttt ccaactgtgg agaaataaat
     2041 agagatactg aaaaagatat gaacaatgtt gaaaatatca taaaacgcca aggagcttga
     2101 ataatattta aaggagtatt ttgtaaggca aaccatggaa atatttttgt acaaagtaga
     2161 atagatatta tacccattat ataagtgatt ttagataaga ttcctaattt tttaaagtta
     2221 aatgttagta taattaaaca aataagcaaa gttaagccaa taccaaatga tagtaattct
     2281 gaatctatac ttccaatcaa aaactcttta cccagataaa ttgctctttt ttgtaatata
     2341 tccacataag gtctattcag atcttgatac aatgtctgct caatcatagg acctataaac
     2401 gctagtgaaa gaagtaaagt aactat
//
LOCUS       DACCZY010000204         2411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCZY010000204 DACCZY010000000
VERSION     DACCZY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.213"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(138..428)
                     /locus_tag="IVZ67_002799"
     CDS             complement(138..428)
                     /locus_tag="IVZ67_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166811.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVZ67_002800"
     CDS             complement(546..806)
                     /locus_tag="IVZ67_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166812.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVZ67_002801"
     CDS             complement(943..1356)
                     /locus_tag="IVZ67_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8166813.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVZ67_002802"
     CDS             complement(1812..2297)
                     /locus_tag="IVZ67_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166814.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          890 a          388 c          381 g          752 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa t
//
LOCUS       DACCZY010000205         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCZY010000205 DACCZY010000000
VERSION     DACCZY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.214"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            158..2020
                     /locus_tag="IVZ67_002803"
     CDS             158..2020
                     /locus_tag="IVZ67_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8166815.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2020..>2350
                     /locus_tag="IVZ67_002804"
     CDS             2020..>2350
                     /locus_tag="IVZ67_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8166816.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          845 a          349 c          459 g          697 t
ORIGIN      
        1 aaaaacgctt tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta
       61 gttaaaaagc tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta
      121 gaaaacgctt atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga
      181 aaggcagctt ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc
      241 tgaaaaagaa aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg
      301 gctgaatgag cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat
      361 tagtagctct gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat
      421 atttgcagat caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga
      481 agctattact ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa
      541 tgatttagat gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc
      601 gcgaaagggt ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat
      661 tcttcaaaaa gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg
      721 cggtgagact gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt
      781 taatcaagta atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa
      841 ttacgcggaa ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa
      901 agaggctacg attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct
      961 ttcttatcgc gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga
     1021 atatcattat atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact
     1081 agttgagaat gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc
     1141 acaattgttg acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt
     1201 tgtcaatgaa tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga
     1261 tgcgatcaag caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc
     1321 tttcgtggca tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg
     1381 aagcgttaga gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca
     1441 aaagatcaat gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc
     1501 gcaagaaatg attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg
     1561 catcgatcgt cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat
     1621 tcaacaaata ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa
     1681 tcttagagaa acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt
     1741 gaaagcttat acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga
     1801 atatcgagaa gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca
     1861 acaatatatg gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt
     1921 aggaaaaatc gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag
     1981 tgcattattc tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct
     2041 ttaaaaatta tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt
     2101 gaagagcgat tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt
     2161 aatcacttga atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat
     2221 cgggatttgt tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga
     2281 agtatcggta atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca
     2341 aaaaataatt
//
LOCUS       DACCZY010000206         2347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCZY010000206 DACCZY010000000
VERSION     DACCZY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.215"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..313
                     /locus_tag="IVZ67_002805"
     CDS             <1..313
                     /locus_tag="IVZ67_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8166817.1"
                     /translation="CKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLN
                     ENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIA
                     Y"
     gene            286..963
                     /locus_tag="IVZ67_002806"
     CDS             286..963
                     /locus_tag="IVZ67_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8166818.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            953..2095
                     /locus_tag="IVZ67_002807"
     CDS             953..2095
                     /locus_tag="IVZ67_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8166819.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2108..>2347
                     /locus_tag="IVZ67_002808"
     CDS             2108..>2347
                     /locus_tag="IVZ67_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8166820.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKE"
BASE COUNT          866 a          307 c          471 g          703 t
ORIGIN      
        1 gtgcaaattt atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat
       61 accagtaata aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa
      121 tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt
      181 tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat
      241 aacaaaaaca ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca
      301 aatagcttat taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca
      361 aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat
      421 aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg
      481 gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga
      541 aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca
      601 agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct
      661 gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt
      721 gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt
      781 ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag
      841 aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca
      901 cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc
      961 tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc
     1021 ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat
     1081 tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat
     1141 acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt
     1201 tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga
     1261 ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc
     1321 tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc
     1381 tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc
     1441 agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg
     1501 gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc
     1561 tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc
     1621 accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg
     1681 tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt
     1741 caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga
     1801 tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga
     1861 tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg
     1921 gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa
     1981 tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat
     2041 gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg
     2101 atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact
     2161 cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg
     2221 aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaacag aggcaaaaaa
     2281 tttgtttgaa ataaattcag tttttgtttg tacatcccct ataacccatg aatttattat
     2341 aaaagag
//
LOCUS       DACCZY010000207         2326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCZY010000207 DACCZY010000000
VERSION     DACCZY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.216"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..692
                     /locus_tag="IVZ67_002809"
     CDS             <1..692
                     /locus_tag="IVZ67_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8166821.1"
                     /translation="TWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAEN
                     AWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIA
                     GNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQP
                     VVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRD
                     AGAVGGTLYAT"
     gene            682..1422
                     /locus_tag="IVZ67_002810"
     CDS             682..1422
                     /locus_tag="IVZ67_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8166822.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1419..>2326
                     /locus_tag="IVZ67_002811"
     CDS             1419..>2326
                     /locus_tag="IVZ67_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166823.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKI"
BASE COUNT          879 a          283 c          444 g          720 t
ORIGIN      
        1 atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca
       61 tgattaatac atggaataac attaaggact ctttctggaa tattgttact ggaattgtta
      121 attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg
      181 taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca
      241 ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag
      301 gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa
      361 gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata
      421 aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac
      481 ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta
      541 gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg
      601 ttcgaaagat tactacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg
      661 gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat
      721 tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga
      781 gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac
      841 aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag
      901 gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt
      961 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
     1021 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
     1081 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca
     1141 gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
     1201 attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa
     1261 ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta
     1321 tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt
     1381 cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc
     1441 ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg
     1501 aaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta
     1561 gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag
     1621 agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta
     1681 tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg
     1741 actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg
     1801 acatacgact ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg
     1861 ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta
     1921 aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga
     1981 ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa
     2041 caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg
     2101 gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc
     2161 agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt
     2221 actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg
     2281 ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattag
//
LOCUS       DACCZY010000208         2314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCZY010000208 DACCZY010000000
VERSION     DACCZY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.217"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            91..609
                     /locus_tag="IVZ67_002812"
     CDS             91..609
                     /locus_tag="IVZ67_002812"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8166824.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            737..1420
                     /locus_tag="IVZ67_002813"
     CDS             737..1420
                     /locus_tag="IVZ67_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8166825.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1729..1802
                     /locus_tag="IVZ67_002814"
     tRNA            1729..1802
                     /locus_tag="IVZ67_002814"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1763..1765,aa:Arg,seq:tct)
     gene            1961..>2314
                     /locus_tag="IVZ67_002815"
     CDS             1961..>2314
                     /locus_tag="IVZ67_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8166826.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIF"
BASE COUNT          777 a          354 c          466 g          717 t
ORIGIN      
        1 aaagggaaac atttttacag ctataatttg aaaaaattta tcgttattat atgattaaga
       61 aggattgaat aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata
      121 agttctataa cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga
      181 atttttggtt tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa
      241 ggaaatctga tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt
      301 acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac
      361 aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta
      421 aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt
      481 tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca
      541 ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa
      601 tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca
      661 tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt
      721 gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact
      781 attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga
      841 agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt
      901 tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc
      961 atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt
     1021 cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt
     1081 gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt
     1141 tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt
     1201 gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct
     1261 cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct
     1321 tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt
     1381 gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg
     1441 tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt
     1501 aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc
     1561 tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt
     1621 gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg
     1681 acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct
     1741 cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga
     1801 cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat
     1861 attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag
     1921 gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat
     1981 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
     2041 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
     2101 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
     2161 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
     2221 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
     2281 tgggctagct ctgttggcat tgctttctat tttc
//
LOCUS       DACCZY010000209         2186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCZY010000209 DACCZY010000000
VERSION     DACCZY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.218"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVZ67_002816"
     CDS             complement(<1..132)
                     /locus_tag="IVZ67_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8166827.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..939
                     /locus_tag="IVZ67_002817"
     CDS             541..939
                     /locus_tag="IVZ67_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8166828.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            923..1617
                     /locus_tag="IVZ67_002818"
                     /pseudo
     CDS             923..1617
                     /locus_tag="IVZ67_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          818 a          337 c          423 g          608 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctctcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaacagag
      541 atgacgatct ttgaaacggt tgtaacagat gttggtatag aagcagaagt attcaggaaa
      601 gaaaaaataa tgattttatt tggagatgaa gcaccggaaa cattaacaga ttattgttat
      661 aagattgaat tgaatcaggt aagaaaagaa ataacttgtg atatgaagct ctgttttgat
      721 aaacaaatgt atgtaatcac agcagtagga gatgttgtcc aaactaattt gaatgaatta
      781 ggacatatca cgataaaatt tgatggatct catgagccgg aacttccagg aacaatgtat
      841 gtagaagata aggaaatgcc gcatttaaga aaggggacaa tcattaaaat cgatcaagag
      901 gatcagaatg ggggaaagct atatggaaat catgcttgat acagcaaata caaaaagtat
      961 ttattcttgt caacagtttt tgaatttaac aggcgtaaca acaaatccta caattctgaa
     1021 aaaagaagga gcgtcaaact tcttctcgca tatgaaagaa atcaaaaaaa taatcggaca
     1081 gattcctatc catattcaga caataggaag aacggaagaa gaaatgatag aggatgctcg
     1141 agttatcata gaagaactgg gaaaagatac gtttatcaag gtgccggtga atcaagcagg
     1201 ccttcaagcg atcaaaaagt tgaaaaaaga aggttatttg attaccggta cagctattta
     1261 cacagaatta caagggtatt tagctattaa taatggagca gattatattg caccttatta
     1321 taacaggatg caaaatcaag gcattgatgc tatgaaagtg attgattcgt tgcggactga
     1381 gatcgaacgt acgcaaagac caacaaaaat attagcagct agtttcaaaa attgtgaaca
     1441 agtcactaca gccattcgat ctggagctca gcgataacgt taggtaaaga gctactagat
     1501 caagtattag agatgccctc gattgataaa gcagtgaggg gcttccaaga agattgggtg
     1561 caaactttcc aagcagaaac gattggtgaa ctaaaaaagg tcgctgatta cttctgaatc
     1621 aatttctcca taaaataaac gatagccttc tcgaaaactc gaggaggcta tctgacgatt
     1681 caatattcat taatttaaag tttgggtgac tattactaac tattacttat ttctcattta
     1741 aacgatgtat tccctaacct ctaatacatc cttgtaactc tgaagttacg ccagttttga
     1801 ataaatttca taaaaacaaa tgaaaagtgt cgcataaaaa tatctttcgt gtttctctag
     1861 tataacagaa taagaagaac aagtccagaa gggatatttt tagtcttttt taaaataact
     1921 tttcagtcga gtttgagttt atgatagttg tttttgaagc gatacggcaa gtgaaaaaat
     1981 agggaagcat gaaagaatct acttaatggc gcattgattc atgtgataga aataaaaaac
     2041 ttttttaaat tatatcctca tgattgtaac aaatccagtg atttgttttg gtacaaataa
     2101 aaaaaggccc cgatgaagtt gtggcaaaag tggtttgcct caaaaataag aaggaatttc
     2161 caaaaatcac ttctcaaatt ttgcca
//
LOCUS       DACCZY010000210         2164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCZY010000210 DACCZY010000000
VERSION     DACCZY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.219"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(129..1055)
                     /gene="cysK"
                     /locus_tag="IVZ67_002819"
     CDS             complement(129..1055)
                     /gene="cysK"
                     /locus_tag="IVZ67_002819"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8166829.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1195..2082)
                     /locus_tag="IVZ67_002820"
     CDS             complement(1195..2082)
                     /locus_tag="IVZ67_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8166830.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          599 a          485 c          361 g          719 t
ORIGIN      
        1 acgtaaaaca gtcgcgttca ctattctacg tctctattca aataattttt ctcatcataa
       61 atttttgtgt gttcttttca tctttaaaag ttttttacat aaacctatct ggagaaaaac
      121 caagtgcctt atatctcttg gtaaagggct gtagataaat aacgttcacc attgtcggga
      181 accacagcta ataccttctt gccttttcct aattcttttg cgacttttag cgcggcagca
      241 attgcagcgc cagatgagat gcctactaaa atcccttctt ttcgcccaac ttctctagct
      301 gtttccatcg cttcgtctcc actaatagaa aggactttat catacacaga agtatccagt
      361 gttttaggaa caaaacctgt accgattcct tggattttgt gaggtcccgc ttctttccct
      421 tctaaaatag cagattctgc tggttctacc ccgattaatt cgattttcgg atacacacgt
      481 tttaattctc ggccagcgcc agtgatcgtt cctcctgtac cgatacctgc aacaaacgcg
      541 tccaagccat ttactccaaa tgcttgatgt atctcaggac cagtcgtctt ctcatgcaca
      601 agaggatttg cttcattttc aaattgtaat ggtaaaaagt agccgttttc ttttgccaaa
      661 cgttctgctt ctctgattga tccagaaata ccatccgcac caggagtcaa aatcaactct
      721 gcgccgtacc ctttcatcaa taaacgtctt tcgatactca tcgtctcagg catcacgatg
      781 attactttat atccttttgc aactcctacc atcgacaatc cgattcctgt atttccagaa
      841 gtaggttcaa tgatagtatc acctggtttc agtagaccat cgtgttctgc tttttctatc
      901 atacttaaag caatacgatc ttttacactt cctcctggat taaaaaactc aagctttaca
      961 aaaacatctg ctgaatcttc tggtacgatt ttatttaatt tgacgattgg cgttttgcca
     1021 atcaattcgg tgactgagtt atagatagct gtcatcttca attcctcctt gtctttgctc
     1081 ttcttactta tcataataca atctgttcat ttatggcgag tgattcgtct ttgactagca
     1141 aaaaaaagac aaagatatac catatatggt aatttctttg cctttttatc taaatcaaac
     1201 gtttagttct tgaccaacta ataaagaata actggacata gacatgccat ttttcgccat
     1261 tagatcatca acagacatgc caaatttttg tgcaatatcc catagcgtat cacctgacac
     1321 gactgtatat gtctgaccat tatttgtatt tacatctgtt gttgaagatg atgacgttga
     1381 agtcgtcgta gtagtcgttg ttgttgtact tgtggttgtt gtagccgaac ctgttgatgg
     1441 cgtatcaaaa cgggttaaat tataagtatt aatcaatgag ttcaatttgg ccccataatt
     1501 tggatcggta gcataacgac ctgtcaagta cgctgttgca tcataaaaac ttgttgtatt
     1561 gcttttccac acgccagcat aatgataatc gcccgtggaa aaactggttg aacgaagaac
     1621 attcgcatgg tcttggaagg attcccaata agaagaatat tttcggaatg gctcattcat
     1681 cgttacccat tgtccgttta catattcttt ggttgccata taaacgacct gtccgttata
     1741 gcttcctttc acaccgaata gattataatt tggcgcacta gctaatccgg aagtgccata
     1801 tgcactttcc aacaacgctt gagcgatcat gactgaagca tacaagtcat tacttgcagc
     1861 aacttctgac gcagaccagc cgatttgatc gataaacgct tgaggatcag cgactgtttg
     1921 agatccttgt tcatctgcat gaacatgttg cggtaccgct gtaaccatca tcggcccaac
     1981 taaaagactc gttccaatta aaggagcaat cgctttttta ttgatcacca tttttctttt
     2041 aggtctatgg tgccgggcac tgcgggtctt cagctcttgc attcaaattc ctcctcaaaa
     2101 taaaaaaact taataaaaag ttctcttctt aaaacaaact taattaatta aattatactt
     2161 ttaa
//
LOCUS       DACCZY010000211         2063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCZY010000211 DACCZY010000000
VERSION     DACCZY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.220"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..273)
                     /locus_tag="IVZ67_002821"
     CDS             complement(<1..273)
                     /locus_tag="IVZ67_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8166831.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDT"
     gene            complement(478..828)
                     /locus_tag="IVZ67_002822"
     CDS             complement(478..828)
                     /locus_tag="IVZ67_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166832.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1423..1869
                     /locus_tag="IVZ67_002823"
     CDS             1423..1869
                     /locus_tag="IVZ67_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8166833.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT          635 a          328 c          425 g          675 t
ORIGIN      
        1 cgtgtctaca aggcgggcaa tctcaaatcg catactttga taatgtttct gtttgcgttt
       61 taaactgtca tattgtcctt gagcggataa ttcatcatag ttttttcttt tttcaagagt
      121 aatatcattt tcataaatcc aagctaattt ttgttttcgt tccagattta cttcgttttc
      181 gatcgtttta cgtttgggag taatgattgc ttcataatca taaatttcaa tatacgtggg
      241 agtttcaatg attttctgat tataagcttg caaaaatcat cactccttgt tgcgtattcg
      301 attgtcaaag aaccagtcat tttctcctga tgttgatcta taaatcaagt taagggggaa
      361 ggcctgcgta gcagcccttc tagcctatgg ggtgttgggc ggtcttaacg gacagaaacg
      421 gctctgtggg cgctgccccg ccgtttcgtc ctaaccaccc aacactccat agtatagtta
      481 gttttgtgta gtggtacctt gttttctggg aatcaaagtt gccgtatcag cggttgccga
      541 taacccataa ctgacaaatt gtccattcga gcgtccccaa ggtacaatca ccaaattggt
      601 aaatgtaatt atggctaaac cttgttgggt aagttcttcc ggcgtgatca caggatgttc
      661 agatttgact ttgacactga ttttttcgta gcgaagtttt ggtaggatac attcgtagct
      721 ccaacctaga atttcgccct ccttttgcca gcgagtgaca ttgacgactt ggtattcttt
      781 ttcgagtgtt tcttttggta aactgagatc ggtaattttt attggcatca caattctcct
      841 tttttatacg aaagtttata cgatttgatt aaaaaatata gatggccatc tacgacttgt
      901 attatatacg agaacttata cgtagtcaag agattttcta aaaaaatata tacgattttt
      961 tatacgagtg ttataatgaa aaaaatgata aggaggaatt tgaatatgtt tggtttagta
     1021 caacctaata aagtgaaggt gggccaacga atcaaagaaa taaaagacgg aatgtctctc
     1081 tcttttacag aattagggaa tcgattaggt ttaaaaaagc cgacgatcag ttcctacgtt
     1141 caaggctatg cgttggctcc taagagtgtg atcaaccaat tatctagtat cagcggaaaa
     1201 cctgtcggct ggttttattt tgggcatgtg gaagaatata tccgagagta tttgtttctc
     1261 attggtcaga aaaaaatagt ggacgattat cctgaaatca ttgagaaaat aaagcaagaa
     1321 ttttatacgg gcgagttcaa aaatcctgct tgggagaatg agctaggcta tcctttggag
     1381 gaatttatcc aagaatgttt tgtagaatat tcgagtgaaa tcatgaagga ctatgtcgta
     1441 cagattacga aagaggtact tgaagaatcc aaaacgctaa acaaactatc atcaaaagca
     1501 aaagaagaag ctgtcacgat tctttcatct gggatcattg actatatcga gatgagtggt
     1561 gagatcaagt atggagagaa agatcgcatt gcgaaaatgg tgaaagaatc aataaaaacc
     1621 tttgatgtga aacaagacct gacatttaat gattcctatt tgattggaaa gttgatcaat
     1681 attcttggta cagatgaaga tacggagctg tttctttctt ctttatcaat gatatttact
     1741 gataagcatt tttccactcg atttggtggg gaagaattag tcgaaatatt tcaatcgatg
     1801 cgaccagcgt taatcaaact ctatcgggag aaaacaccag atgaatttta tgattggttt
     1861 gagaagtaaa ataagaaaag tggcttagat tacaagttgg tgaaaaaatt tttttcagca
     1921 taacatcagt aatctataaa ggggttgttg attatactgt agaagttaag tactcaagag
     1981 attttttgta cgtaaacaaa gcaaaaaagg gtgcagtttc actagaaaat gtactctttt
     2041 ttgtgatttt tcttagttat aga
//
LOCUS       DACCZY010000212         2060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCZY010000212 DACCZY010000000
VERSION     DACCZY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.221"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>2060
                     /gene="acm"
                     /locus_tag="IVZ67_002824"
     CDS             <1..>2060
                     /gene="acm"
                     /locus_tag="IVZ67_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8166834.1"
                     /translation="SLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEK
                     KQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKN
                     LDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTG
                     DMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYR
                     GEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFEN
                     HSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTF
                     ELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELK
                     FSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDA
                     ETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNE
                     NKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPK
                     YRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTER
                     EKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVI
                     IGLL"
BASE COUNT          807 a          340 c          405 g          508 t
ORIGIN      
        1 atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac
       61 ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc
      121 gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
      181 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
      241 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
      301 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
      361 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
      421 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
      481 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
      541 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
      601 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
      661 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
      721 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
      781 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
      841 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
      901 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
      961 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
     1021 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
     1081 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
     1141 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
     1201 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
     1261 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
     1321 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
     1381 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
     1441 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
     1501 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
     1561 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
     1621 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
     1681 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
     1741 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
     1801 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
     1861 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
     1921 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
     1981 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
     2041 tgtaatcatt ggtttattga
//
LOCUS       DACCZY010000213         2049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCZY010000213 DACCZY010000000
VERSION     DACCZY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.222"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            29..886
                     /locus_tag="IVZ67_002825"
     CDS             29..886
                     /locus_tag="IVZ67_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8166835.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            887..1435
                     /locus_tag="IVZ67_002826"
     CDS             887..1435
                     /locus_tag="IVZ67_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166836.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1695..>2049
                     /locus_tag="IVZ67_002827"
     CDS             1695..>2049
                     /locus_tag="IVZ67_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166837.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
BASE COUNT          786 a          265 c          440 g          558 t
ORIGIN      
        1 atattgctaa aagaaaagag gtaggaacgt ggtagcgaag aaatatgtaa tcggtaattt
       61 taaaggtggc gtaggtaaaa gtacgtgcgc gcaaatgtta gggtttgaat cagcagttaa
      121 tgaaggaaaa agaaccttga taattgactt ggatatgcaa ggaaatactt ctgatgtgat
      181 gaacttaacg catatgaact tcactaacga agaaggaggc ggagaaggtg aaccgattgt
      241 ttacgaaaat actgtgactg acgtgttgat aaacaatctt ccacctaaag aggctgtcta
      301 taaagtaatc aataacctct atatacttcc agcagacatg tcctttgaaa tgtatgatga
      361 ttggatcaaa gaccaatata atgattcatt atcccaattc aaatatatga aagaaaagct
      421 agaaccatta tttgatgagt ttgacgtcat ctatttagat gtcccacctt ctatttctgt
      481 atatagtaaa tccgcgatgt acattgcgga ttgggcaatc gtcgtcttac agacccaagt
      541 aaaatcgatg agaaatgcga tgcaatatct tgagtatatg gacttttttg tgaaagaatt
      601 tgatacaaat ttatatgttg ctggtgttat tcctttcatg ttggaaagcg gtgacgcagt
      661 agatcaggaa atgtataaac aagcccaaga gatttatggg gaacatttga ttaaaaatgt
      721 tgtactcaaa aatgcgcgct taaaacgata cgacggatca ggtattacga tggagaagac
      781 aaagaaaggt ctcttaaaac aatgggatcg caaaagccac gaattattta tcagtatttt
      841 gaaagaattg gaagaacacg agaattggta tgcggaggtg gagtaattgg ctgacatcaa
      901 aggacttgat atttttggga acaacaaagg actcgccaaa ataaaaaaag aagcagaagc
      961 aaagaaaaag cagcaagcaa ttacaccatc tgaaaataag caattagaag tgcaccaagt
     1021 tcaagaaata aaaacttcct ccaatgttga aagacagagt agaggaagaa caaaaaaagt
     1081 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
     1141 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
     1201 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
     1261 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
     1321 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
     1381 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
     1441 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcgt
     1501 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
     1561 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
     1621 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
     1681 aggaggagat gtagatgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
     1741 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
     1801 aaaggattat gagccaatta gatgactctc aaatagagta tgtacccaat gagctaattg
     1861 tcagtttaaa ggctacacaa agaatttcaa cagagaaaaa taaaccgcaa aatttattgg
     1921 gtgtaaaagt tatggtggaa aaagatttga ctcttaggat aaatgaaagc gattataata
     1981 agcgagatca agtattatta gtgaaattga acgaagaaat tagtgtggaa gaggcagtaa
     2041 gaacattta
//
LOCUS       DACCZY010000214         2049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCZY010000214 DACCZY010000000
VERSION     DACCZY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.223"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            130..681
                     /gene="lepB"
                     /locus_tag="IVZ67_002828"
     CDS             130..681
                     /gene="lepB"
                     /locus_tag="IVZ67_002828"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8166838.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(764..1306)
                     /locus_tag="IVZ67_002829"
     CDS             complement(764..1306)
                     /locus_tag="IVZ67_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8166839.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1543..1941)
                     /locus_tag="IVZ67_002830"
     CDS             complement(1543..1941)
                     /locus_tag="IVZ67_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8166840.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          726 a          353 c          333 g          637 t
ORIGIN      
        1 tcaaaaaagt tttttgtcca atagcctaat tgaaagaatt atttttattt cagctatcta
       61 gtcgcatttt tttgatattt ttcttataat aaagaaagaa tgaaaaaaag agggatggat
      121 tgatagagat tgacaaaaaa acaacgctat attaatcgct tctggcttgt attcaaatac
      181 ttacttgttt ctgtattcct tgcgttcatg ttgcggggat ttttattcat cccggtacca
      241 gtggaaggca attcaatgga aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa
      301 ttttcggaga tcagacgatt tgatattgtt gtttttcaat tagcagatgg cacgatttat
      361 atcaaacgag taatcggtct tccaggagaa aatgtcagct accaaaatga tcaattgaag
      421 atcaatggga aagtagttaa ggaaccttat ctaactaaaa acctgaaatc agatcatgca
      481 aatgcatcct atacgacgga ctttacacta caagagctaa cgggacaatc caagcttccg
      541 gaagataatt attttgtact tggagataat cgaagagttt caaaagatag tcgttcgttt
      601 ggcacgatca ataaaactga tattctaggt aaagcaagat ttgtctatta tccacttgat
      661 gaaataaaat ggatccagtg agaaacaata aagcgtgcag tctgttgaga ctgcacgctt
      721 tattgtttaa tccaaaatca gtttactacc ttagtacgta aaatcaaaca gctcgtttaa
      781 acacttttct agcctctaaa gttttgataa atgaataaac aaccattatt tgaataggga
      841 tttggatcat attcctgatg aatctaccta gaaaaattac tttgaaatct gcaccaaaca
      901 aaacagacat cagccaagaa ttgataaaaa ggctgactgg gatcataatc aataattggg
      961 cagcgatgac ggaaggcagg tttatctttt tatgatataa aaataagcca ttaataaatc
     1021 caccaaggaa agccgtgata agaaaagcag cttgaaacgt tcctgaacca aatatcgtga
     1081 aatttaaagt gttcgacaac atttcaagca aaccagccca gatcggtcca tatagataac
     1141 ccataagcgc catacttaaa aatccgaatg tgatttgaaa ataagcagga ccaatcgcta
     1201 tcttgtataa gatgacatca agggctaata aaagagcaga ggtcgacaac aatcttgcat
     1261 tcatttcggg caatctaaaa acgtcttttg caatcaaagt gttcaaaata ttctctcctt
     1321 tgtcagttaa taataaaagg ataccataaa atcaccaatg tgtcatgaca cattggtgat
     1381 ttacatacac tggcatctaa caattaaaaa aagggtgaga agactgcttt tataaactgt
     1441 aaaaaagaga ttgtacgaat aaaaaaaata gaggcggaga atacttatcg tatcatccgc
     1501 ctttgttttt ttgttataaa aaacgtcaga ttagttatct gcttacatct tcatccatac
     1561 gggttctaat gtatttccgt ctagatcttg gacttctaat gaaaacattt cttcttcagg
     1621 cattcccata tctacttcgt ggaaagtacc accattttct tttgcaattt gagcaaattt
     1681 ttttacttca tccgcatttt caacactaaa cgaaatcaaa gcaccacttg tttttttatt
     1741 atcagctatt tctttatctt ttataaattt tttataaaaa tcatgagaga gaagcattat
     1801 ccaaaaagtg tcatcccata ccattgaacc agcttcttca gtagaaaatt cttcattttt
     1861 tttaaaaccc agcttttcat aaaaagcaac ggaacgattg acatcagcaa caggaaaatt
     1921 aacaaaaacc atactattca tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc
     1981 ttatttagga aacgctgtca aaaatttaga ttcatatata gaaaccaaaa taaaaatttg
     2041 catcatcaa
//
LOCUS       DACCZY010000215         1986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCZY010000215 DACCZY010000000
VERSION     DACCZY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.224"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            599..931
                     /locus_tag="IVZ67_002831"
     CDS             599..931
                     /locus_tag="IVZ67_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8166841.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1550..1891
                     /locus_tag="IVZ67_002832"
     CDS             1550..1891
                     /locus_tag="IVZ67_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8166842.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          688 a          265 c          421 g          612 t
ORIGIN      
        1 ttttttttga gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa
       61 aatcttgata ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata
      121 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttttgcactt ttggatgata
      181 aatttggaca attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa
      241 ccttcgtcag cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt
      301 gttacggtat tacccgcaat ggatttagta taacagatat tcaaaagtga taccactttt
      361 tttggctgtg ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat
      421 gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg
      481 catgtttgga tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt
      541 tgaaaaataa caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg tttattttat
      601 gtcatataca ggaattttga gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc
      661 tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa
      721 gaaatatata atccagaaag acaaagtctc agaggaaatg atttattaca ttgggaaaca
      781 aaagcaaata attttaaaag atcctatccc cttaaaagag ctatatccaa caatcaaaca
      841 tgtttatgac caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac
      901 cgcaaaaggg atggggcgtt tgatcggctg aattgatcat tctcataact tgtattttat
      961 cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa ggaacttaga gtcactagaa
     1021 aaataaggag tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt
     1081 aacgattggt aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg gatagtggtg
     1141 aaatgaattt gtgtaaataa taagcgcaga gtaaacaaga gggaatttag aagttagaca
     1201 agttagaata gcgtctagat taatcatgag cagctactaa gggatctaga ctttgacgca
     1261 aactcatgga ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc
     1321 gtgtgctgca gtacatgtag atacttgcga ggacgttgat tattggatca atcgagcatt
     1381 taccgctgtc tactagggtt tctcgtttcg gacgccataa acgagtcgaa attttttatg
     1441 tatttttaag taaacgaaaa acgataaaat atgaacaagt cctgaataaa gtgaaaaaat
     1501 tgatataaaa catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta
     1561 tgatccgaat atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta
     1621 caaaggattt gtgacgttta gaaggggcgg aaattgtaaa ggattagatg ttttagcatt
     1681 agacgaggat gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct
     1741 acctgaagat gatgaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga
     1801 cgaattatct gttgaagata cttggtcata tttgagtgat tatattgtca gctttgaaat
     1861 cattgaattt gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa
     1921 ggcttagaag gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag
     1981 gaattt
//
LOCUS       DACCZY010000216         1862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCZY010000216 DACCZY010000000
VERSION     DACCZY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.225"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(306..761)
                     /gene="tnpA"
                     /locus_tag="IVZ67_002833"
     CDS             complement(306..761)
                     /gene="tnpA"
                     /locus_tag="IVZ67_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8166843.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1004..1862
                     /locus_tag="IVZ67_002834"
                     /pseudo
     CDS             1004..1862
                     /locus_tag="IVZ67_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          588 a          361 c          352 g          561 t
ORIGIN      
        1 gttcgataat ccagtgattt tctaggaaga ttatttctgc gccagcattt tctaatgttt
       61 gaatttgtgt tttgtaatct tggttgcttg tgctaattct tgaatagccg taaattgcca
      121 aaatttcatc accctttctg gacataagtt tctgacacca gttaaaatca agtctatcag
      181 taacccatac acccgtgagg acgggtgtat ttttaatccc cctagaaggg gtcgacactc
      241 gctgcccctt aaaaggggat ttttaaaaga ccgccaactg ctcatcttca ctcacttact
      301 tctctttatt ttttaaatgg ttctacgtac tctcttttac ttataatata tcgaatgcga
      361 tcatcagctt cttgttcacg gatgtatttt tcatttgtct tttgattcaa tccgactgta
      421 ctcacatagt atctcttcga ccaaaaggat ttgtttccgt atttatattt gagatttgcg
      481 tgctgctcgt ggattagtac cgcactacga cctttcagat accccataaa actggatata
      541 catatttttg gtggtactcg aacaagcatg tgaatatgat ctagcattgc atgtgcttct
      601 atgatttcca catctttcat ttcgcacaac ttcctaagta tttttccaag atcttttcgt
      661 aattgcccat agattacttt tcgttgatat tttggtgtaa aaataatatg atacttacaa
      721 ttccaacgag tatatgctaa acttttatcg tcatttgaca tgaaaaaact cctttcgatc
      781 tgatgagcgg ttggtagcca ctcactagta tatcgaaagg agtttttatc gctaaagata
      841 cttcttccac gcgtagaaac gcgcggtttt cttgtttcgc acgctttacg cacaaaactg
      901 actgaagtca ttaagaaaat aaaagggtct aaaactttta agttttgtac cgacccccaa
      961 aagttagacc aaaaaatcta acttttggag gtcggtattt tttatggcta agtatacttt
     1021 tgaacttaaa ttaaagatcg ttcatgatta cttggatggt aaaggcggaa gtgattatct
     1081 ggcaaagaaa tattccatta aggctccatc acaagtgaag cgctggatca atgcctatca
     1141 agaatttggt gaagagggtc tagtaagaaa gcgacaaaag aaaaaatatt ctgttcaatt
     1201 taagctggat gcgatagagt tatatctaac aagtgagctg tcctatcgtg aagttgccaa
     1261 cacacttaac atgaacaacc ctaatttgat cgctagttgg atgcgtcaat tccgtgaggg
     1321 tgggatcgat ggactctcaa aaacgaaagg atgtccacct atcttgtcta agaaaaatga
     1381 acccaaaaat aagaaaaaat ctacaactaa agcaacgtct aaagaacgtg aacgcatcga
     1441 ggagttggag aaacgagtcc gttcccttca aattgaaaat gcctttttaa aagaattgag
     1501 gaagctgcga aaacaggaag cccaacaacg acgaatgaag caatcgcacg aatcatcgca
     1561 agcctccgag gaccgtttaa attagttgag cttctagaaa ctttaaagtt ccctaaggca
     1621 atctttatgt actggcaaaa acgattggac cgtaaaaatc cagatcaaga gattgaagcg
     1681 gaaatgctta aagttcggga gaagcacaag gattatggct gtttacggat gaccaatgaa
     1741 ctaagaaacc gcggtttctc tatcaataaa aagaaagttc agcgcctgat caagaagcta
     1801 ggaataaaag taacgacata cacacgtaaa tcacggcgct acaattctta tcgaggacaa
     1861 gt
//
LOCUS       DACCZY010000217         1837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCZY010000217 DACCZY010000000
VERSION     DACCZY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.226"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            258..854
                     /locus_tag="IVZ67_002835"
     CDS             258..854
                     /locus_tag="IVZ67_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8166844.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(912..1394)
                     /locus_tag="IVZ67_002836"
     CDS             complement(912..1394)
                     /locus_tag="IVZ67_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166845.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1500..1697)
                     /locus_tag="IVZ67_002837"
     CDS             complement(1500..1697)
                     /locus_tag="IVZ67_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166846.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          628 a          365 c          276 g          568 t
ORIGIN      
        1 cataaaaaaa agaagataga gatttaataa ttatatatca gctgtttttt tcaaataagc
       61 aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac ataacgattt
      121 ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc tgtttactta
      181 ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa aacgcaaatc
      241 aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc cgttcaatct
      301 atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg atcaaagaag
      361 caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt atccttttcg
      421 gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca ttgactccag
      481 cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt tatggtacag
      541 tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt gaattatatg
      601 cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct aatacttggg
      661 tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca gttattgacg
      721 aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg caattagtat
      781 tcggttcacc agaaacaccg gctgaggaaa aagaatacat gaacgatgcc gatcgcttcc
      841 gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac atcttgtcgc
      901 aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc gtttaagcgt
      961 tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat cggtcctcgc
     1021 catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg atataataca
     1081 attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa aacaagtcct
     1141 tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc tgacaagatc
     1201 ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat gactagtgac
     1261 actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata aataatttct
     1321 tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa tagaaccgta
     1381 aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag gaaaagaata
     1441 ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa ttgttttctt
     1501 tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa taattcttgg
     1561 tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt tgatacaact
     1621 tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc tcctaaatta
     1681 atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc acattaccac
     1741 tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa tcaaccttac
     1801 aggcattata gcgtactgtt ttcgttttca caattaa
//
LOCUS       DACCZY010000218         1827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCZY010000218 DACCZY010000000
VERSION     DACCZY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.227"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            77..1642
                     /gene="guaA"
                     /locus_tag="IVZ67_002838"
     CDS             77..1642
                     /gene="guaA"
                     /locus_tag="IVZ67_002838"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8166847.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          560 a          379 c          388 g          500 t
ORIGIN      
        1 aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac
       61 taagttttat tcaaatttgt cttcaccaac acgatttgac gaagagccaa aaattatcgt
      121 acttgattac ggtagtcagt ataaccaact gatcactcgc cgtattcgtg agttcggtgt
      181 tttctcagag ttgatgagcc accgcattac cgcagaagaa gtaaaggcaa tgaaccctaa
      241 aggaatcatc ctttctggcg gaccaaacag tgtttatgat gagaaggcct ttggtattga
      301 tgaaaagatt tttgatttgg ggatcccagt tttaggaatc tgttacggta tgcagttgat
      361 tacttataaa ttaggcggaa aagttaaacc agccaaaaac cgtgaatacg gaaaagcaga
      421 attggaaatc acttctgcag aagcggccct tttccaaaac actcctgaaa aacaaaccgt
      481 ttggatgagt catggtgacc tagttaccca agcaccgact gatttcgatg tcgttgctac
      541 cagcaaagat tgccccatcg catcgatcca aaatgcagaa cgcaaaatgt acggaatcca
      601 attccatgca gaagttcgcc attccgaata tggaaatgac ttgttgcgcc attttacact
      661 agatgtctgc caatgtactg gtgactggac aatggataac ttcatcgaca tggagatcgc
      721 aaaaattcgt gaacaagtcg gtgacaaaaa agttttatta ggcctttcag gcggagtaga
      781 ttcctctgtt gttggtgttc ttttacaaaa agcgatcggc gatcaattaa catgtatctt
      841 cgttgatcac gtattgttgc gtaaaggtga agcagaacaa gttatggaaa gtcttggcgg
      901 aaaatttggc ttgaacatta taaaagtcga tgccaaagaa cgtttcttat caaaacttgc
      961 tggcgtgtct gatccagaag aaaaacgtaa aatcattgga aatgaatttg tatatgtctt
     1021 cgatgatgaa gcaaccaaac ttgccggtga agaaggcgtt tctttccttg cacaaggaac
     1081 cctttatacc gatgtgatcg aatcaggaac tgaaactgcc caaaccatca aatcacatca
     1141 caatgtaggt ggattacctg aagacatgca attcaagctg atcgagccat tgaacacatt
     1201 gttcaaagac gaagtacgtg aattaggtac ccaattaggc atgcctgaca gcatcgtttg
     1261 gcgccaacca ttccctggac caggcttagg tattcgtgtc cttggtgaat tgacagaaga
     1321 aaaattagaa atcgtccgtg aatcagacgc tatcttgcgc gaagaaatcg ccaatgccgg
     1381 tttagatcgc gacatctggc aatacttcac cgtcctacca ggcattcgct ctgttggggt
     1441 tatgggagat ggccgtacgt atgactacac tgtaggtatc cgtgctgtca cttctattga
     1501 cggcatgacc gctgactttg cccgtatccc ttgggaagtc ttgcaaaaaa tcagtgtccg
     1561 tatcgtaaat gaagtcgcac acgttaaccg tatcgtgtat gatattacca gcaaaccacc
     1621 tgcaacagtg gagtgggaat aaagagccaa cataatatgg atgaggaata cctataaatc
     1681 aacattattc aattactaaa gttcctgatt ttcgtaaaaa ataagatggt ttcctaccat
     1741 tttccctacc aaagcccttg ttagattaac tactaacaag ggctttgctt ttattcacta
     1801 cctttatttt cctattgaat tagcttt
//
LOCUS       DACCZY010000219         1690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCZY010000219 DACCZY010000000
VERSION     DACCZY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.228"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            34..456
                     /locus_tag="IVZ67_002839"
     CDS             34..456
                     /locus_tag="IVZ67_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166848.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            466..669
                     /locus_tag="IVZ67_002840"
     CDS             466..669
                     /locus_tag="IVZ67_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166849.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            880..1464
                     /locus_tag="IVZ67_002841"
     CDS             880..1464
                     /locus_tag="IVZ67_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166850.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          592 a          241 c          309 g          548 t
ORIGIN      
        1 gttttcttat ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta
       61 ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt
      121 acaaatgaac caactactgt agtagataat aaccaagaat taactccttt agataggaac
      181 ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt
      241 tctccattac ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt
      301 gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat
      361 ggttattggt actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg
      421 attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg
      481 aacacaccaa atttttatca agatagcgat attctaagta aaattaattt accgaaagaa
      541 gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt
      601 tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa
      661 gaaaaataat gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag
      721 tgttcataaa atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac
      781 aattcatttc atgttcataa acccataaca atatctatgt tcagttaaac tatatggata
      841 aagttatgtt acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg
      901 gtatctactg gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt
      961 gaaaaaattt ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg
     1021 gccattgatt ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt
     1081 agaaatatgg aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt
     1141 catagtattc aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt
     1201 catttgtttg cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca
     1261 ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa
     1321 caagatttgg aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg
     1381 gaacggtgga acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa
     1441 aatggaaaga caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta
     1501 tctagaaaag tgacaatagt atgagaattt tttgtaagtg tctactgata acttattctc
     1561 aaatgttgta aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc
     1621 gtactctaat ttgcagtctc tcactctttt agagtgagag tttttattag attttcaaat
     1681 taattagtaa
//
LOCUS       DACCZY010000220         1676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCZY010000220 DACCZY010000000
VERSION     DACCZY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.229"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            231..587
                     /locus_tag="IVZ67_002842"
     CDS             231..587
                     /locus_tag="IVZ67_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8166851.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            597..1496
                     /locus_tag="IVZ67_002843"
     CDS             597..1496
                     /locus_tag="IVZ67_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8166852.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          557 a          289 c          348 g          482 t
ORIGIN      
        1 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
       61 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
      121 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
      181 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
      241 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
      301 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
      361 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
      421 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
      481 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
      541 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
      601 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
      661 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
      721 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
      781 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
      841 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
      901 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
      961 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
     1021 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
     1081 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
     1141 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
     1201 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
     1261 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
     1321 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
     1381 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
     1441 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
     1501 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
     1561 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
     1621 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttga
//
LOCUS       DACCZY010000221         1674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCZY010000221 DACCZY010000000
VERSION     DACCZY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.230"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(325..>1674)
                     /locus_tag="IVZ67_002844"
     rRNA            complement(325..>1674)
                     /locus_tag="IVZ67_002844"
                     /product="16S ribosomal RNA"
BASE COUNT          394 a          477 c          365 g          438 t
ORIGIN      
        1 cggttgggtt ttgatccttc ttctagcgat agaagttaga tcaagaaaat aagcaattga
       61 acttattaaa aaactctatc cagctcacaa atcagcctgt acggcaaaaa gatcgatcgt
      121 ctcataaaat caaagatttt atggccgtcc gcctattttg cttcaggcta aacaatttgt
      181 tcagctttca aattcaacgc ggtgttctcg gtttgtatta cttacattta cttcaaatat
      241 ccagttttca atgaacaaaa gtatttgaga gtaaacctct caaaactgaa caaagtaaaa
      301 acaaattgtg tagtctccgt aatattcctt agaaaggagg tgatccagcc gcaccttccg
      361 atacggctac cttgttacga cttcacccca atcatctatc ccaccttagg cggctggctc
      421 caaaaggtta cctcaccgac ttcgggtgtt acaaactctc gtggtgtgac gggcggtgtg
      481 tacaaggccc gggaacgtat tcaccgcggc gtgctgatcc gcgattacta gcgattccgg
      541 cttcatgcag gcgagttgca gcctgcaatc cgaactgaga gaagctttaa gagattagct
      601 tagcctcgcg acttcgcgac tcgttgtact tcccattgta gcacgtgtgt agcccaggtc
      661 ataaggggca tgatgatttg acgtcatccc caccttcctc cggtttgtca ccggcagtct
      721 tgctagagtg cccaactgaa tgatggcaac taacaataag ggttgcgctc gttgcgggac
      781 ttaacccaac atctcacgac acgagctgac gacaaccatg caccacctgt cactttgccc
      841 ccgaagggga agctctatct ctagagtggt caaaggatgt caagacctgg taaggttctt
      901 cgcgttgctt cgaattaaac cacatgctcc accgcttgtg cgggcccccg tcaattcctt
      961 tgagtttcaa ccttgcggtc gtactcccca ggcggagtgc ttaatgcgtt agctgcagca
     1021 ctgaagggcg gaaaccctcc aacacttagc actcatcgtt tacggcgtgg actaccaggg
     1081 tatctaatcc tgtttgctcc ccacgctttc gagcctcagc gtcagttaca gaccagagag
     1141 ccgccttcgc cactggtgtt cctccatata tctacgcatt tcaccgctac acatggaatt
     1201 ccactctcct cttctgcact caagtctccc agtttccaat gaccctcccc ggttgagccg
     1261 ggggctttca catcagactt aagaaaccgc ctgcgctcgc tttacgccca ataaatccgg
     1321 acaacgcttg ccacctacgt attaccgcgg ctgctggcac gtagttagcc gtggctttct
     1381 ggttagatac cgtcaaggga tgaacagtta ctctcatcct tgttcttctc taacaacaga
     1441 gttttacgat ccgaaaacct tcttcactca cgcggcgttg ctcggtcaga ctttcgtcca
     1501 ttgccgaaga ttccctactg ctgcctcccg taggagtttg ggccgtgtct cagtcccaat
     1561 gtggccgatc accctctcag gtcggctatg catcgtggcc ttggtgagcc gttacctcac
     1621 caactagcta atgcaccgcg ggtccatcca tcagcgacac ccgaaagcgc cttt
//
LOCUS       DACCZY010000222         1670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCZY010000222 DACCZY010000000
VERSION     DACCZY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.231"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..510
                     /locus_tag="IVZ67_002845"
     CDS             <1..510
                     /locus_tag="IVZ67_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166853.1"
                     /translation="KNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSI
                     FSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPS
                     MMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAEL
                     RKLVEYGII"
     gene            497..>1670
                     /locus_tag="IVZ67_002846"
     CDS             497..>1670
                     /locus_tag="IVZ67_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8166854.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYT"
BASE COUNT          543 a          218 c          270 g          639 t
ORIGIN      
        1 aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt
       61 tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa
      121 gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg
      181 acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta
      241 cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat ttctttttca
      301 ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat
      361 gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt
      421 gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta
      481 agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc
      541 cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca taactattcc
      601 tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt
      661 tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag
      721 ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact
      781 ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt
      841 tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat
      901 tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc
      961 ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt
     1021 tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt
     1081 agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag
     1141 atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc
     1201 acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc
     1261 ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg
     1321 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
     1381 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
     1441 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
     1501 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
     1561 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
     1621 aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa
//
LOCUS       DACCZY010000223         1668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCZY010000223 DACCZY010000000
VERSION     DACCZY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.232"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..897)
                     /locus_tag="IVZ67_002847"
     CDS             complement(<1..897)
                     /locus_tag="IVZ67_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8166855.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRY"
     gene            complement(1056..1322)
                     /locus_tag="IVZ67_002848"
     CDS             complement(1056..1322)
                     /locus_tag="IVZ67_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166856.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1312..1668)
                     /locus_tag="IVZ67_002849"
     CDS             complement(1312..1668)
                     /locus_tag="IVZ67_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8166857.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          473 a          324 c          204 g          667 t
ORIGIN      
        1 atacctttta aatttcttat agtcctctga aatcaaaata tagcgtgatg gatactcatc
       61 aaaagaagaa tcgtattctt cctttaaaat tcccgattta cttaattttt ctagtacatc
      121 aaatactcta tcactcaagt aactccttaa attgttatca ctttcccatt gaactacttt
      181 tctataagtt gaattatcag ttgaaaagat agaattttca tccataaacg catatattag
      241 cagcaatctt tcatcattgt caaattctat tggatcaaaa atatctaagt tgtcatcaaa
      301 atctttatca gttccggtaa cttttttgtt aattactgaa ggtgtcttgt aagaaataga
      361 atctaaatca aagcgttttg attctaaatt attttgtgct ttctcaatta aataattcac
      421 tttttttgtg agaaaccttc tgatattgtc tttgctatat gttccataaa aacccttatc
      481 ttctaaagag tccctgtact tcttaacttc tatatactct tcagccaaaa cttgtggaac
      541 tggttcatct aagaaaaaaa cccctacctc gcctttgcta tttttataaa agtaatcaat
      601 ttctgctata gttcccgatc tataattatc aactggagaa cccaacttat ggccaaaaat
      661 cgctaataat atatcactat tttctacaat ttgtttatta ataacacttt gcccagagct
      721 attatttata taagtaggaa tcgcattcgt ttcccatcga attggcatca aaattacttt
      781 tgtattatct gtattttcta tattccatct atatatggtt tcttctactt cattcctctt
      841 tgctgctata tcacctggtg atgctataaa tacttttatt agatttatgg ttttcattta
      901 ttgtaccttt ctctttatgt ctctttaaag taatttttgt ataaaattta ttgactttta
      961 cactatagga caacttttat ctatttagta caaaaaggcc cactaaacta attgattagt
     1021 taggagccta ttacaggtat attttcaact cgcttttaag caaattctac taccagcttt
     1081 ttgcctaatg ccctagcgat atcatcaagt ttatcgaaag atacgttatc ccctctctca
     1141 attcgagcaa tagtagattg agttgtatgt gctttttgtg caagctccgc ttgagttagg
     1201 cctgcttcct cccgtgcatg ataaagagca accgcagttt cgagttttct tccttcacgg
     1261 tcatatgctt ctgcaaaatc tttgtctttt tgcttttctt caatcaattt tttaatcatc
     1321 atttgtgaac cactcctctc taagttcttt tgcatgttta agttcactta ttggtgtttt
     1381 ctccgttttt ttagtaaatc catgggtaat gacatacctt ccattgacca catggaaata
     1441 gatagctctt tggatgtttg atcctacctt acttcttaat tcgtacaaat tagtgtctaa
     1501 ttttttcacc catttcaacc gttgagctac tagcatccca ttcttttctg tttcttctat
     1561 tgtacgtaaa agcttcgcac tatctttttt tggtaatgtt tccaaccatt ctaaaaattc
     1621 atcatgacca ttaggtcttt taaatgtttc aaaagatatt ttttccat
//
LOCUS       DACCZY010000224         1665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCZY010000224 DACCZY010000000
VERSION     DACCZY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.233"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            85..1659
                     /locus_tag="IVZ67_002850"
     CDS             85..1659
                     /locus_tag="IVZ67_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166858.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT          627 a          362 c          328 g          348 t
ORIGIN      
        1 gctatggtac aatttttata tcgttaagaa agacttaaat atatttagat atttattaaa
       61 ttaagccagt cggaggaatg aagagtgaaa aagagtttaa tatcagcagt aatggtatgt
      121 tcaatgacct tgactgccgt agcatcaccg atcgctgccg cggcggacga ttttgattct
      181 cagatacaac agcaagatca aaagattgca gacttgaaaa atcaacaagc agatgcacaa
      241 tcacaaatcg atgccttaga aagccaagtt tctgaaatca acacacaagc acaagaccta
      301 ttagcaaaac aagatacatt gcgtcaagaa tctgcacaat tagtaaaaga cattgcagat
      361 ttacaagaac gtattgaaaa acgtgaagat acaatccaaa aacaagcacg cgaagcacaa
      421 gtaagcaaca caagctcaaa ctacatcgat gctgtattga atgctgattc attagcagat
      481 gctattggac gcgttcaagc aatgacaacg atggtaaaag caaacaacga cttgatggaa
      541 caacaaaaac aagataaaaa agctgtagaa gacaaaaaag ctgaaaacga tgctaaacta
      601 aaagaattag ctgaaaacca agctgctttg gaatctcaaa aaggcgatct tttatctaaa
      661 caagctgatt tgaacgtact gaaaacttca ttagctgctg aacaagcaac tgctgaagac
      721 aagaaagctg acttgaaccg tcaaaaagca gaagctgaag cagagcaagc tcgtattcgt
      781 gaacaacaac gtctagcaga acaagctcgt caacaagctg cacaagaaaa agctgaaaaa
      841 gaagcacgcg aacaagcaga ggctgaagca caagctactc aagcttcatc tactgcacaa
      901 agctcagcaa cagaagaatc atcagctaca caatcttcaa tgactgaaga atcatcatca
      961 gctacacaat cttcagcaac agaagaatca acaacacctg aatcttcaac agaagaatca
     1021 acagcgcctg aatcttcagc aacagaagaa tcaacaacag cgcctgaatc ttcagcaaca
     1081 gaagaatcaa caacagtgcc tgaatcttca gcaacagaag aatcaacaac agtgcctgaa
     1141 tcttcaacaa cagaagaatc aacaacacct gcgcctacaa caccatcaac agatcaaagt
     1201 gttgatacag gaaacggcac aggaagtagt actccggctc caacgccaac accaacacct
     1261 gaacaaccaa aacctgtaac acctgctcca gctccttctg gaagtgtgaa tggtgcagca
     1321 atcgtagcag aagcttacaa atatattggt actccatatg tttggggcgg taaagatcca
     1381 agtggctttg actgctcagg gttcacacgc tatgtttaca tgcaagtaac tggtcgtgac
     1441 atcggtggtt ggacagttcc tcaagaatca gctggaacaa aaatttcagt ttctcaagca
     1501 aaagctggag acttgttatt ctgggggtca caaggcggaa cttaccacgt agcgattgct
     1561 ttaggcggag gacaatatat ccatgctcct caaccaggcg aaagcgtaaa agttggttca
     1621 gttcaatggt ttgcccctga ctttgctgtc agcatgtaag aatga
//
LOCUS       DACCZY010000225         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCZY010000225 DACCZY010000000
VERSION     DACCZY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.234"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVZ67_002851"
     CDS             131..505
                     /locus_tag="IVZ67_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8166859.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVZ67_002852"
     CDS             complement(619..1248)
                     /locus_tag="IVZ67_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8166860.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVZ67_002853"
     tRNA            complement(1382..1454)
                     /locus_tag="IVZ67_002853"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACCZY010000226         1642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCZY010000226 DACCZY010000000
VERSION     DACCZY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.235"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..1464
                     /locus_tag="IVZ67_002854"
     CDS             <1..1464
                     /locus_tag="IVZ67_002854"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8166861.1"
                     /translation="EQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDI
                     LGYAISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGT
                     DEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVS
                     DMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLA
                     KMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSR
                     FNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGS
                     IYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQK
                     ILETYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIR
                     QEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1457..>1642
                     /locus_tag="IVZ67_002855"
     CDS             1457..>1642
                     /locus_tag="IVZ67_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8166862.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAI"
BASE COUNT          561 a          279 c          356 g          446 t
ORIGIN      
        1 gaacaagtgg ttcttttagc agatgaatcc ttagaggaat atgatacggt aagcaaacaa
       61 acgatgttgt atcgcgaatt actttcagat gaagaatcaa aagaagatct gattgcaaca
      121 atcgttgata ttttggggta tgcgatttcg ccagagtatc ttttcaacgt cttagcggat
      181 caagcaaaac aagcaacgtt ccaattgaat gatctaaaca aagcctttgt gcaattggca
      241 tctacctaca atcaatttaa tgggttattt gatgatgtgg atctccaatc aaaaaaacta
      301 ggaacagatg agcaacaacg aaatgtgacg attaccgaag tcattaagaa attgaatgac
      361 gtggaagttt taggtcacga tggggacgtg atcggggatg cttatgaatt tctgatcagt
      421 caatttgctt cagaagcagg gaagaaagcc ggagaatttt acacgcctca tatggtttct
      481 gatatgatgg cacaaatcgt gacgttggat caaaaagaac gacgattctt tagtgttttt
      541 gatccaacca tgggttcagg ttcattgatg ttaaatgtgc gtaactactt aactcaccca
      601 gataacgtta aatatcatgg gcaagaatta aatacaacga catacaatct agcaaaaatg
      661 aacttgattt tacatggggt agatgccgaa gaaatgaatc tccgcaatgg ggatacgttg
      721 aataaggatt ggccaacaga tgaaccttac acatttgatg cagtagtaat gaacccacct
      781 tattcggcta attggtcagc agatacgacc ttcttagacg attctcggtt taatcgttat
      841 ggaaaattag cgccaaaatc caaagcagat tttgcgtttc ttcttcatgg tttctatcat
      901 ttgaaggaaa cagggactat ggccatcgtc ttgccacacg gagttttatt ccgtggcgct
      961 gcagaagggg tcattcgtca aaaattacta gaagacggca gtatttatgc tgtgatcggt
     1021 atgcctgcga acttgttctt cgggacatcg attccaacga cggtgattgt attgaaaaag
     1081 aaccgccaaa ctcgagacgt attattcatt gacgctagtc gagaatttgt caaaggaaaa
     1141 aatcagaata aattatcgga agaaaacatt caaaaaatcc tcgaaaccta tgctgaaaga
     1201 aaagatgtcg agaaatacgc tcatttggca aactttgatg aaatcaaaga aaatgactat
     1261 aacttaaata ttccacgcta tgtagatacg tttgaagaag aagaaccaat tgatatggtt
     1321 catgtcggaa atgacatcaa gaagatacga caagaacagc aagtgctaga aaaagaactt
     1381 ttagaagcta tctcttccct gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg
     1441 ctggaggtgt tcaaacatga gtaatgatat acaaccagaa attcgtttcc caggatttac
     1501 ggaagcttgg gaacagcgta agtttgaatg cttacttgat aaaaaagatg gagtaaggag
     1561 gggaccattt gggagtgcct taaagaaaga attctttgta tctaatagca attttgttgt
     1621 ctatgaacaa caaaatgcta tt
//
LOCUS       DACCZY010000227         1621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCZY010000227 DACCZY010000000
VERSION     DACCZY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.236"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..265)
                     /locus_tag="IVZ67_002856"
     CDS             complement(44..265)
                     /locus_tag="IVZ67_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166863.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(363..608)
                     /locus_tag="IVZ67_002857"
     CDS             complement(363..608)
                     /locus_tag="IVZ67_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166864.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            848..1462
                     /locus_tag="IVZ67_002858"
                     /pseudo
     CDS             848..1462
                     /locus_tag="IVZ67_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          553 a          282 c          249 g          537 t
ORIGIN      
        1 catatagtaa ccaaagagtt aataaaataa aaagagcgaa ccattataga tcagctcttt
       61 tttcattgct gtttttaatc ttatctagaa tcacttcaaa tgtttcaaag gtatttacat
      121 ctaactgaca taaccaaagc aagtacttct tttctttgta agtccaatca atattacggt
      181 aagaggacat tagattatgg taccttgctc tttgttcaac tgaagccaat tcccagcgtt
      241 tttcaaatat tttttcttct tccattattg atccctcgca agacgctacc tcaaacttaa
      301 ataatgggca gcgaaagtct aattccttag atctaagtat agcaaacaaa tatgaaaaaa
      361 acttagaagg tagtaaaagt tcttccgtct tttgctttgc ttctattctt aatgtgtttc
      421 gttatcggtg ccctacaaag attttcatta attacacact catttattgt tgtgtatttt
      481 ctgacatttc tctcttgatc aattatttta attccatggg catcctgata gttcatagga
      541 gaggagcggc tatcagaaat agttgcacct atctcttgaa cttcttttac aacttctgga
      601 aaatccatcc atcctgcttc agctgtcaaa agtaataatc tataatattt atctttcctt
      661 ttcaatctaa gaaatcgtca ccccattctt tttcttctat tataaactat ttaactttta
      721 gaattcaaaa accgtttgtc tatttagagt ttaccctaaa tagacaaaaa tataacattg
      781 aaaaatttag agttctaacg tatgattagg gtgtattata aacgttatag aaaagtaggg
      841 aataaatttg aaaattgctt atattagagt ttcatccatc gatcaaaatg aacaacgtca
      901 aattgaagaa atgaaaaagt ttggcgctga aaaaattttt atcgaaaaac aatctggagc
      961 gacgattagt catcggccga ttttccagga agctttagat tttgtcagag agcaagatat
     1021 ttttattgta gaagccattg atcgacttgg acgcaattat gacgagatta tcgattcagt
     1081 gaattatcta aagaagaaga atgtgcgact tattatcacc agtttaccga tcatggctga
     1141 agcaattgga aatcctctcc tagataaatt catcaaagac ttaatcattt aaatctcagc
     1201 catgattgct gaacaagaac gtactgaatc gaaacgacgc caggcacaag gaattaaaat
     1261 cgctaaagca aacggcgtct ataaaggtcg tcctaagtta tacagtgcag acaccaaaga
     1321 tcctcaacgt cgccttgtct ataggagtat cgttcaggat cttgagaatg gggttgctat
     1381 ctcaaagatt gcaacagact acaatgtgac taggcaaaca atttaccgaa taaaaaaaga
     1441 aatcgaccaa ctaattgtat gatttctttt ttgaatcacg taattattaa ttagctttgt
     1501 catcaataca gacaatcatt ttagattcat ctagctaagt tgattacatt tatgttaaaa
     1561 agaatttagt taacataaat ataattgatg cataaatcgt ccttatttaa aggcatttaa
     1621 c
//
LOCUS       DACCZY010000228         1604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCZY010000228 DACCZY010000000
VERSION     DACCZY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.237"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            82..>1604
                     /locus_tag="IVZ67_002859"
     CDS             82..>1604
                     /locus_tag="IVZ67_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8166865.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          583 a          266 c          327 g          428 t
ORIGIN      
        1 gtagaaacat atgcagagta ttcaccggaa cagatcaatc gtatgatcga tcaaatggag
       61 tctgtcaaaa aagaaagtta tttggaccct actgtttttg aagcagagga acatatccaa
      121 aaagtcattc ataaaatgtt ccgtccagac aatgcctata caaaatttga ttttcgaaat
      181 ggccgtccaa cgaagtctgc gatcttaacc acgagttcca ttgatatggc caaaaagtat
      241 tatcgggcca tcaaagagat gacgaaagaa tctgattggt taataaaaga gtttcccaat
      301 cagcctatcc gtgaaggacg aacgatggag gatcctgatt tcccacgaat cgctattacc
      361 tattcattag aagaaaatag ccgagattca agtgcccaac aagaagaaat gcaaaaaatt
      421 attgaggaat ataatcagta ttatgatact gcctggtcac tagcagacat tgagcgttat
      481 aacggagaca tcaataatcg tttggcacga aaaagagcgg aattcaaaca atttgggaaa
      541 caaatcgatt tagtaatcgt agtggatcgt ctattgacag gttttgacgc ccctacgatt
      601 caaaccctat ttgtggatcg taatctagag tatgcaggat tgatccaagc cttttcacgt
      661 acgaaccgta cgtatcctga aaaaacaaaa ggactaattg ttactttccg aaagccagcg
      721 acaatggaga agaatgtcga ggatgcgaca aaactgtatt cagaagctaa agaagaatcg
      781 ggtttgattt atccaagtta ccaagaatcc aaaaaaagat tcaagcaagc atatggtaag
      841 ttaaaagagg tttcatccat ggaatccatt gatgaacata cgccacttga aatccgtatc
      901 gagtacgtca aagcatttca agaattgaat cgtgcgtatg aatcgttagt gacttatgat
      961 gattacaatg atgacatggt gcaatcttca actttgaaca atcaaattca acttttggaa
     1021 gaacaaattg gcgtgtacga gaccgtgaag ggttcattga tggaggaaga accagaaaaa
     1081 gagggagaag atttttcaac gatcgaattt tatagtgaaa ataccactaa gttgtatgat
     1141 attgattcta cttatattga tcaattacta ggaacttatg ctgcgaatag ttcagatgca
     1201 cgagaagaaa ttgaaaaagc tttggctaaa ttgaataaaa cagaaggagt caaagaagtt
     1261 taccgacaaa ttttaaatgc atttgatgtg gggactttag atagtaatga agatatcttt
     1321 gtcattaaac gtcgctattt tacacaagca agtgataatt tgattcatgc cttttccaat
     1381 gagtggtttg tttctgagaa agaattacat gcttctaatc tgcaatatat gccaggtgaa
     1441 gatccaattc ctaatatgaa agcaattatc aacagtaagg attacgaagg atataaagcc
     1501 aaacatccag aagcgaaacc atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa
     1561 atgttagatg atgagttgat tccacttgaa aatgaattga ggta
//
LOCUS       DACCZY010000229         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCZY010000229 DACCZY010000000
VERSION     DACCZY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.238"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1097)
                     /locus_tag="IVZ67_002860"
     CDS             complement(<1..1097)
                     /locus_tag="IVZ67_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8166866.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1586)
                     /locus_tag="IVZ67_002861"
     CDS             complement(1227..>1586)
                     /locus_tag="IVZ67_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8166867.1"
                     /translation="KLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTG
                     DIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIIS
                     SFNILRRNPKELLIDNN"
BASE COUNT          492 a          320 c          266 g          508 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattt
//
LOCUS       DACCZY010000230         1579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCZY010000230 DACCZY010000000
VERSION     DACCZY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.239"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            190..453
                     /locus_tag="IVZ67_002862"
     CDS             190..453
                     /locus_tag="IVZ67_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8166868.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            453..725
                     /locus_tag="IVZ67_002863"
     CDS             453..725
                     /locus_tag="IVZ67_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8166869.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            802..1131
                     /locus_tag="IVZ67_002864"
     CDS             802..1131
                     /locus_tag="IVZ67_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8166870.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1121..1407
                     /locus_tag="IVZ67_002865"
                     /pseudo
     CDS             1121..1407
                     /locus_tag="IVZ67_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          580 a          202 c          303 g          494 t
ORIGIN      
        1 ttttttgttg atttaccaac atgtttagac attttcctct aggaaaaaat taactagcac
       61 cacgggtata aaggaaacgg ataacacttt acaatgctgt tttggtactc caatttaaac
      121 aaaacaatcg aatttacgta cttaattaag tacgttagaa tggagatata aattagaggg
      181 ggagtagata tggcaaaaac agtcttaaat cctagctcag cacggaaaaa tttttataag
      241 ttgttgaaag atgtaaatga gaatcatacg gaaattgaaa tcattagtga gcgcagtgaa
      301 aataatgcgg tgcttattgg tttggatgat tggaaagcaa tcaaagaaac gctattactt
      361 gagcaaatag gcactttggg tgttgttcgt aagcgagaaa aagatgatag tggatttact
      421 aatgttgatg aaattgattg ggataactta taatgaataa ctattctgtg atgattaaaa
      481 actcggctaa agctgattta aagaaaatca aacagtctaa tttgaaacct caatttgaga
      541 aggttattca gactttaaag gaagatccct atctccctac ccaatcattt gagaagttaa
      601 aacctacaca tgaaggtagg tattctagaa gattgaatag acaacaccgg gttgtttata
      661 aggtagatga agatgaacac gtggttgaaa tttattcagc aaggacgcat tacgagtcac
      721 agtaattact tgtaaataca ttatctcgtg gtataatgta tttactaagt aattacatac
      781 tgttagaaat gagggattta aatgattaca aaaacaagaa aacaaggcaa ttcaatcatg
      841 ttgacagtac ctaaagattt taatgttcct aatggagtcg aagttgaagc aaagttggta
      901 gagaatggta ttctttatga atttgttgaa ccacaaaaag aattttatga ttttagcgaa
      961 gatattttgt ctgatataat tgcagaaggc tacgataaag atgagattct tgtagaattt
     1021 aagaatagaa aaaacaaaat gcattcttcc tttagagata ttgctgaaga tacacttact
     1081 aattctaaag taatgactaa agaggagttg gcaacagaaa ttggcttatg aaatcgttcc
     1141 ttcaaaacat gtcattaagt atttaaaaag ctaaaagaga agccattgaa agaaaaattt
     1201 ttgaatttaa tttatgatga aattgcgatt gatccatata agggtgaaca aaaaactggg
     1261 gatttatcag gtatttggtc tagtgggttc aaatatgcgg gcacaacata tagagtagct
     1321 tacgagatta tagaaaacaa ggttattcca gtattgttat gtggaacgca tgaaaacttt
     1381 tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg ttcttaagaa ggacggcttc
     1441 ttttttatcg aaaatctgat tcgtctacat gactaaagtc gcgagtattc tctgcttctc
     1501 gataaattaa ataacgtcat tactttgagg aaagctataa atcaatatat ttcagctttt
     1561 ccttatttat gtactacct
//
LOCUS       DACCZY010000231         1540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCZY010000231 DACCZY010000000
VERSION     DACCZY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.240"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..225
                     /locus_tag="IVZ67_002866"
     CDS             <1..225
                     /locus_tag="IVZ67_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8166871.1"
                     /translation="LKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQRLYTF"
     gene            331..>1540
                     /locus_tag="IVZ67_002867"
     CDS             331..>1540
                     /locus_tag="IVZ67_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8166872.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          478 a          308 c          292 g          462 t
ORIGIN      
        1 cttaaaacaa tttcatctac gttacctcag tcgtttaaaa agaaattcac gatcttccat
       61 cgctaccaat cgggtatttc aaatgccttt aaggtgtccc actcaaacgg cgtcactgag
      121 gggctgaata acaagatcaa gctgattaaa cgaattgcgt ttggttatcg caacttttat
      181 aactttcggg cacggatcta tttacaacgg ctctatactt tttgatgttg gtgaacttca
      241 ccaacatctt ttttgtataa aaaaatatag aaaagacacc ctgaaatcct ttagaattaa
      301 tttggctacc aattcatagg aggattcaag atgtctcata ctcagcttat caaagatacc
      361 ctaaatattc tagacctaaa tatctatttc gaggaaaatt gtttaactaa agaaaaatac
      421 aaagggcaaa tttgcttgat ttacaaagga actcttctgt acaagccaga agagtgttcc
      481 cattgtcttt gtgtcgtccc ctctaggatc attcgttggg ggacgacaac cgttcggttg
      541 ttactgaatg atgtgtctga gtatcgcact tatctggagc tgaagaaaca acgatttaaa
      601 tgtaagtctt gccaaagaac attcgtagcg gatacctctg ttgctgaaaa acactgtttc
      661 attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac ttccatgacg
      721 gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat gaagcgattc
      781 taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt tgatgaattc
      841 aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg acaaagccat
      901 gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta tttttctcgg
      961 ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta tgcgccttat
     1021 gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg tttccacatc
     1081 gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac ggtacgactt
     1141 aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta ttggaaactc
     1201 ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc cagttttaaa
     1261 gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc caacttacgc
     1321 caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga tcagtcacta
     1381 tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac aaccacttta
     1441 cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag ctattcaaac
     1501 ggaccattgg aatgcttaaa caaccacatc aaagtattaa
//
LOCUS       DACCZY010000232         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCZY010000232 DACCZY010000000
VERSION     DACCZY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.241"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            24..1490
                     /locus_tag="IVZ67_002868"
     CDS             24..1490
                     /locus_tag="IVZ67_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8166873.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          498 t
ORIGIN      
        1 tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc
       61 cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta
      121 tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga cttggattac
      181 cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta
      241 tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg
      301 gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt
      361 ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca
      421 tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt
      481 acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag
      541 aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac
      601 aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt
      661 tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg
      721 ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata
      781 caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa
      841 aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag
      901 caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg
      961 aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga
     1021 atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg
     1081 aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt
     1141 acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt
     1201 ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt
     1261 tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt
     1321 tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg
     1381 gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc
     1441 tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca
     1501 taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DACCZY010000233         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCZY010000233 DACCZY010000000
VERSION     DACCZY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.242"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..237
                     /locus_tag="IVZ67_002869"
     CDS             <1..237
                     /locus_tag="IVZ67_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166874.1"
                     /translation="LKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIA
                     FHQLIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            482..1507
                     /locus_tag="IVZ67_002870"
     CDS             482..1507
                     /locus_tag="IVZ67_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166875.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
BASE COUNT          558 a          209 c          311 g          446 t
ORIGIN      
        1 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
       61 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
      121 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
      181 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
      241 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
      301 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
      361 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
      421 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
      481 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
      541 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
      601 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
      661 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
      721 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
      781 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
      841 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
      901 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
      961 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     1021 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     1081 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     1141 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     1201 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     1261 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     1321 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     1381 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     1441 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     1501 cgattaaaaa agtaaacatc caaa
//
LOCUS       DACCZY010000234         1512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCZY010000234 DACCZY010000000
VERSION     DACCZY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.243"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            239..919
                     /locus_tag="IVZ67_002871"
                     /pseudo
     CDS             239..919
                     /locus_tag="IVZ67_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            981..>1512
                     /locus_tag="IVZ67_002872"
     CDS             981..>1512
                     /locus_tag="IVZ67_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166876.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMI"
BASE COUNT          546 a          231 c          310 g          425 t
ORIGIN      
        1 atttatttaa gcatatataa atataattga aataggacat aaatatatga gtatgagaaa
       61 ggtgattcca tgtttaataa atgggcaaac cacccaatga actcgagagg tagagataat
      121 ttgaaggaga cggacaagtt ctgggaagaa gtatatagta aaaacggttc tgttgcaaag
      181 ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg acagagtaat
      241 gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg ttggttacta
      301 cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc gtggaataaa
      361 tgtttgtcat actacgattt atcgttgggt ataagagtac agcaaagtcc tctattatct
      421 ttggaagaag aaaaatagac aatccttcta ttcatggaaa atggacgaaa cctatatcaa
      481 aattaaggga cgttggcatt atctttatcg tgcaattgat gcggacggct taaccttaga
      541 tatctggtta cgaaagaaac gggatacgca agcagcctat gctttcttaa aacgactcca
      601 taaacagttt ggtgagccga aagcaattgt gaccgataaa gcaccttctc ttggctccgc
      661 ctttagaaag ttacagagtg tgggtttata tactaagaca gagcaccgaa ctgtgaagta
      721 tcttaacaat ttaatagaac aagaccatcg acctattaaa cgacgaaata aattttatca
      781 aagtctccgt acagcctctt ccacgattaa gggcatggag accattcgag gaatatataa
      841 aaagaaccga agaaatggaa cgctcttcgg cttttcggtg tctactgaaa tcaaggtatt
      901 aatgggaata acagcctaag atatttggag ttcagagagg gcgcgtttga ttttcaaact
      961 tcgcaacaga accgagaacc ttgggttttc aagtgctttt tggttcatta atcttaacca
     1021 tagtgttttt aaagtggaaa acaagcaaca aaaaaagtat gtggagcctt gtaggaatag
     1081 ttcttattat ttttactgtg actgcagcaa cagaagcaaa ttattcgcat aaaattcagt
     1141 cgtataaagg tcgtttagtt atgaccaaaa aagatgttac acgttataca tcctcaagaa
     1201 ctggaggcct atatgactat gcaccattag atgcattgaa atataaagat catttaaaga
     1261 aacatgaagt aaaagttggt gatcggtggg agaatgcatc tcataaagca tataattcca
     1321 aaatagtcta tacagtagaa gatgccgatg agcaaaatat cacattacct gtattcaatt
     1381 attggggaac agttgtgaaa gtaaatggga aaaagagcac tatagaaaat gacaatggtt
     1441 tagtgtctat taaaggtacg gggaaaagaa ctatagttga ggtttcatct acgtatccaa
     1501 ctacgatgat tc
//
LOCUS       DACCZY010000235         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCZY010000235 DACCZY010000000
VERSION     DACCZY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.244"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVZ67_002873"
     CDS             64..972
                     /locus_tag="IVZ67_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8166877.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="IVZ67_002874"
     CDS             972..>1480
                     /locus_tag="IVZ67_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166878.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DACCZY010000236         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCZY010000236 DACCZY010000000
VERSION     DACCZY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.245"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(71..143)
                     /locus_tag="IVZ67_002875"
     tRNA            complement(71..143)
                     /locus_tag="IVZ67_002875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Gln,seq:ctg)
     gene            complement(189..617)
                     /locus_tag="IVZ67_002876"
     CDS             complement(189..617)
                     /locus_tag="IVZ67_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166879.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(677..1435)
                     /locus_tag="IVZ67_002877"
     CDS             complement(677..1435)
                     /locus_tag="IVZ67_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8166880.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          417 a          281 c          274 g          483 t
ORIGIN      
        1 aatacaataa tccccctgca aaaagattgt tcttttacag ggggttcatt ttttcagcaa
       61 gcacatcaaa ttgggatggc agggttcgaa cctacaattg cttgattcag agtcaagtgc
      121 cttaccagtt tggctacatc ccatttattc aacaaaataa tcatagcact ttcctaaaaa
      181 aacgcaagtt atttttcttg atcggatagt tgttgcttta ctcgtaaaag gcggcgtact
      241 agtttctttt ctaatccagc aggaagggca atcagttctg ctactgacaa gacggtgtcc
      301 aataactgat attctttgac aggttcattc ataatatgat agatgtttgc taaacgttca
      361 atatattcaa agcgagtata ctctgttcga gcaattagaa atttcaataa atcggtggct
      421 ttctcgtact cttgatctgc gtaataaaca agagattgac gatacaattt gatttccagt
      481 tcattgaggg tagcaggtct gcgacgctcg ttccactcat taatagagac tttaccttga
      541 acatttccct tgcagtcacg gacaaaaata atcggaattt catttggatc cattttttta
      601 tgtgtgaatt tttccattat ctacgctccc ttcaaaggtt tggtatcttg agtataaggg
      661 atttttgttt gaattactag cttttctcca aagttagcat ctgtccaccc attgcagtga
      721 aaatcgccga aaaatcgttg atcggtactg ctcgattttt tacaccatag ggatcgttga
      781 cataaatatt ttctttgtca tatccagtga ctacacaagc atgaacaaga ggagtgactc
      841 ttacttctcc attatttgtg gtccaagtca taaaatcagt ttcatctggg acttcaaaat
      901 tgatagtcac agaagtccag acaggatttc ctttagatag tgcttttttg atatcatcga
      961 gttgagcatc ggtttttgga aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg
     1021 aggcaactgg gggaacatag acgaccatag cccagtctcc gccactgata tcaccaacaa
     1081 atccttcatt aggatcgcca tgagtcccat ctgcattgaa aacagggacg taatccaatt
     1141 gctcagctaa ctggttttta gttgtttcat aaccataaaa agataaaagc atagaaagcg
     1201 aagtgacttc acagccattt tctaaagaag gagaatcaaa ctggctctct aatggaacat
     1261 ctagtaaaac ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg
     1321 cagtgttcat tgtatgttct ggagaatgga tatgtaggta ataacttgta ccgcttaata
     1381 aaagagcagc tgtgccccct agaaacaata gttttttgaa cgtttttttc ttcatttgaa
     1441 atctcccttt tcagc
//
LOCUS       DACCZY010000237         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCZY010000237 DACCZY010000000
VERSION     DACCZY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.246"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..753
                     /locus_tag="IVZ67_002878"
     CDS             <1..753
                     /locus_tag="IVZ67_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8166881.1"
                     /translation="DFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFSGFSDL
                     KIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNI
                     YIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTK
                     DTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYIC
                     DLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            983..1213
                     /locus_tag="IVZ67_002879"
     CDS             983..1213
                     /locus_tag="IVZ67_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8166882.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          492 a          205 c          226 g          502 t
ORIGIN      
        1 gattttattt taaattctgg gaaaactatt atttatagta cgatgagtga tattaagaaa
       61 aacacaaaag ttggcgatgc tactattatt cgcttttgtc aaaaaattgg tttttctggt
      121 ttttctgatt taaaaattga aattgcaaag gaagacttta gtcaaaaaaa agaacatccg
      181 actagtgtaa agtactatga tgaaattgct aatagtttaa cagaagctct ctattccact
      241 attaggcttt tagacgaaga aaaattgaat gaagccatta aaatgatcac tcaatcaaaa
      301 aacatatata tatttggagt gggatctagt ggaaatacta gtcttgattt agaaaatatg
      361 tttcttcgcg taggagttca agcaaaagct gtactagacc ctcattttca agcccaagtc
      421 gcttctttat taacagttaa tgatttagta atcatttttt ctttatctgg gaaaacgaaa
      481 gatacttatg attcattgaa aatagctaaa aaaaatggcg caaaaattat tgctataact
      541 aactatattc attcacctat aggaaagtct gcagacttag ttttacaaac agccattgaa
      601 gaatttctta atggtggatc tttagctgga aaaatatcac aactttatat ttgtgatcta
      661 ttagttcatg gttatgaaca aaacaataat attgattcag ttgaattaag agaaaaagtt
      721 ttgcgctcta ttattgataa aagtattgag tgaactaccc ccacttagct aaacctaacg
      781 gtttttaacg attgaagtag gcaattcttg ggatgagagc atacttgata gcttattttt
      841 tactaatagc ggtggctctt attgaccaaa tatccctgta gttcctacgg ttcttgattt
      901 tatctaaaga tatttttatc gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct
      961 cattatcact agtttatcga gtatggatga agcaatggat catcctctct taaataaatt
     1021 tattaaggat ctgatccttc aaattttttc catgatcgat gagcaggaac ctactgaatc
     1081 taaacgacat cgggcacaag gaattattaa gattgctaag cgaatgtttt ctataaaggg
     1141 tgttctaagc tatacagtgt taattcaaaa gattcccaat catcttgttt ataagaacaa
     1201 cgttgaagat taaaaaagtg tttcaatgct aaaattgcaa aatacttcaa tgttatgaga
     1261 caaactgtct ataagaatca atttccaccc atttcatcac ttgttaaaag tttatgatcc
     1321 ttttgatttt tatattaatt tatctgtatt taatacagat tttatttatt agttcatctg
     1381 cctattgtat tttattttgt tgccttacca tctgaagtaa atata
//
LOCUS       DACCZY010000238         1405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCZY010000238 DACCZY010000000
VERSION     DACCZY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.247"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            20..430
                     /locus_tag="IVZ67_002880"
     CDS             20..430
                     /locus_tag="IVZ67_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8166883.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            444..800
                     /locus_tag="IVZ67_002881"
     CDS             444..800
                     /locus_tag="IVZ67_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8166884.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          517 a          207 c          263 g          418 t
ORIGIN      
        1 attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag
       61 aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc
      121 cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca
      181 agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt
      241 aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat
      301 tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt
      361 ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt
      421 aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa
      481 caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact
      541 tagaagaacg cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc
      601 aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt
      661 cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac
      721 gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac
      781 agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca
      841 gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt
      901 ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata
      961 gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa
     1021 gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga
     1081 aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat
     1141 cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt
     1201 tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gataattact
     1261 tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc
     1321 ctgatgaagt agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc
     1381 ttgattcaat tcaagacaac aaaca
//
LOCUS       DACCZY010000239         1388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCZY010000239 DACCZY010000000
VERSION     DACCZY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.248"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(359..973)
                     /locus_tag="IVZ67_002882"
     CDS             complement(359..973)
                     /locus_tag="IVZ67_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8166885.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          452 a          270 c          231 g          435 t
ORIGIN      
        1 ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt tcatcaacgt
       61 gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt
      121 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
      181 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      241 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      301 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      361 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      421 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      481 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      541 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      601 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      661 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      721 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      781 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      841 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
      901 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
      961 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
     1021 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
     1081 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1141 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1201 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1261 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1321 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1381 tacaaaaa
//
LOCUS       DACCZY010000240         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCZY010000240 DACCZY010000000
VERSION     DACCZY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.249"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            82..>1368
                     /locus_tag="IVZ67_002883"
     CDS             82..>1368
                     /locus_tag="IVZ67_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8166886.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACCZY010000241         1367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCZY010000241 DACCZY010000000
VERSION     DACCZY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.250"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(104..403)
                     /locus_tag="IVZ67_002884"
     CDS             complement(104..403)
                     /locus_tag="IVZ67_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8166887.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            754..>1367
                     /locus_tag="IVZ67_002885"
     CDS             754..>1367
                     /locus_tag="IVZ67_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8166888.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKF"
BASE COUNT          455 a          221 c          195 g          496 t
ORIGIN      
        1 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
       61 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      121 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      181 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      241 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      301 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      361 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      421 aatatatttc ttcccactac attctataaa caaagtatac gctacatcta gattttttta
      481 aatcattact ggattgttaa aacctttcaa tacactgtta tggcaacgat ttttatttta
      541 aaaacattct aaattaaatc agtaaaaatt tttattctaa gatttttatt agaattacgc
      601 ctaatttgtg tctctcaggg caattctaag aacgtttcaa gagatagcga cactatcgtt
      661 atcgaaaaag gcgaaaacct atctcatttt ctgctaatgt aagttggttt agcttttgtt
      721 aatatgactt tatgaaaatg gaggtaatta aatatgattt ttcctaaagt taatgaagca
      781 agcttgaata aaggatcctt ttttcatatc tatgaaaacg aagattttga actgtataat
      841 atcctttcac aaaaagaggt catcgaaaac caaataatct gccatccaat tatttacaaa
      901 tctattttca agtatgcatt ttttgcttcc aatgattttc aaagaatgga gattaccgac
      961 tgtatattcg aaaactgtga tttttcaggc tgtatattga caaaaagcat gttgcatcgc
     1021 gtgaaatttg tcaattgtaa attaacaggt actaaattga tggaaagtta tattgggaac
     1081 acgttattcg agaattgtaa aatggactat gtaaatttat ctggttcaaa cataaaagaa
     1141 agtacctttg agcactctgt cttatcaagc gcagattttg tagactgttc cctcactaaa
     1201 ataatgtttt ctactaatga tttagaaaat gctagctttt ttgatactga tttaaaaaat
     1261 atcgatttaa gtagcaactc ctttgaaatg attgaagtac gctcggaaca tctaaaaaat
     1321 tgtacgctta atcagttgca agcacttggt tttgctagaa aattttt
//
LOCUS       DACCZY010000242         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCZY010000242 DACCZY010000000
VERSION     DACCZY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.251"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1355
                     /locus_tag="IVZ67_002886"
     rRNA            <1..>1355
                     /locus_tag="IVZ67_002886"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACCZY010000243         1335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCZY010000243 DACCZY010000000
VERSION     DACCZY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.252"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            25..1194
                     /locus_tag="IVZ67_002887"
     CDS             25..1194
                     /locus_tag="IVZ67_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8166889.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          488 a          196 c          224 g          427 t
ORIGIN      
        1 attcacagaa aagaggactt ccatatgaac gattttacta cagaaattat ggaaatacta
       61 atcaataaag gtgatttgga tgatttgttt cgtcgtcacc tagaattggc tattaacgct
      121 ttattacaag ctgaattaac ggtatttctt gactatgaaa agtatgatag agctggattt
      181 aattcaggta attcccgtaa tgggaattat tcgcgctctt ttaaaacaga gtacggtgaa
      241 ttgaatttag taatacctag agataggaat ggagactttt ctcaacaaac tttacctgct
      301 tataaaagaa ccaatgactc tttagaaacc actattattc agctagttca aaaaggaatt
      361 actatgtccg aaatctctga tctaatcgaa aaaatgtatg gtcattatta caaaccacaa
      421 actatttcaa atatgagtaa aattgtgtct gaagatgttt cggcatttaa agaaagaact
      481 ttagaagcta aatactcagt catttttatg gatgctactc atattccctt aaaaagacaa
      541 accgtatcaa aagaagctgt ctatatagta attggtatca ggttggatgg aactaaagag
      601 gttctaggat ttactatagc tccaactgaa tctgcttatg tttggaaaga gattcttcaa
      661 gatctaaaag accgtggttt agaagaggtt ttgttagtcg taacagatgg tttaagtggt
      721 attgacgata gtattcatag tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat
      781 atatctagaa atatcgctca taaggttcgt gttagtgatc gacaagaaat ctgtagcgat
      841 ttcaaattag tttaccaagc aagttcaaaa gaagaagcaa ataatcaaat aaggtttatg
      901 atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa tcctgctata
      961 ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac taatttgatt
     1021 gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt tcctaatgaa
     1081 gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa gtttctatgc
     1141 cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt ttaacttgat
     1201 caacagaatg ggcttttcca tttacacaaa attattgacg ctcccgacaa aattatgatc
     1261 ttgcgtaaag ttataaaata ttaataaaaa tattttatta atgaaaagct tggtagcacg
     1321 gttattcata tgcta
//
LOCUS       DACCZY010000244         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCZY010000244 DACCZY010000000
VERSION     DACCZY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.253"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..1170)
                     /locus_tag="IVZ67_002888"
     CDS             complement(76..1170)
                     /locus_tag="IVZ67_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8166890.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          386 a          272 c          198 g          438 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agta
//
LOCUS       DACCZY010000245         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCZY010000245 DACCZY010000000
VERSION     DACCZY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.254"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVZ67_002889"
     CDS             200..742
                     /locus_tag="IVZ67_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166891.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVZ67_002890"
     CDS             832..>1246
                     /locus_tag="IVZ67_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8166892.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCZY010000246         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCZY010000246 DACCZY010000000
VERSION     DACCZY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.255"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            26..898
                     /locus_tag="IVZ67_002891"
     CDS             26..898
                     /locus_tag="IVZ67_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8166893.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          374 a          229 c          301 g          341 t
ORIGIN      
        1 ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa
       61 atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac
      121 aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga
      181 tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac
      241 ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt tcatcggtac
      301 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
      361 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      421 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      481 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      541 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa accgcttagg
      601 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      661 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      721 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      781 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      841 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      901 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      961 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc aagccttgaa
     1021 tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc acttgtattg
     1081 tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct ttttaacaaa
     1141 taggttctgt tgcaaagttt taaatctact atcagataag gtagaataat agaaaaagat
     1201 agcaggagga atgacgatga gtcattttaa aggaaagcaa tttca
//
LOCUS       DACCZY010000247         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCZY010000247 DACCZY010000000
VERSION     DACCZY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.256"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..969
                     /locus_tag="IVZ67_002892"
     CDS             <1..969
                     /locus_tag="IVZ67_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8166894.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IVZ67_002893"
     tRNA            1159..1232
                     /locus_tag="IVZ67_002893"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          413 a          227 c          254 g          350 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gcca
//
LOCUS       DACCZY010000248         1241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCZY010000248 DACCZY010000000
VERSION     DACCZY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.257"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            823..1035
                     /locus_tag="IVZ67_002894"
     CDS             823..1035
                     /locus_tag="IVZ67_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166895.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          460 a          187 c          206 g          388 t
ORIGIN      
        1 tattatcatc gaaacagcta acaaaattgt cataaaaaac gagatggttg atattagaac
       61 ttgtgttttt gtttaggtct agagataaag agagacaatt tccaaatata tgtaatgaat
      121 acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct tttattgatt ttccgagatc
      181 tcatcagtcg ccatttggaa tggttcactc cttatctaga ttcgtttaat gctcggactg
      241 cttgttcgat tgtaatacca aacgcaacgt tattttcgtt ttgtgcatca atcgccataa
      301 aacggttaga agttgtatca aaagctacgc ggtattgcat atttgttttt gtaaacgtca
      361 taaaattcat ctcctttgta atgtaattta gttcttgaaa gtttccttta aactttcgaa
      421 cttataccaa gtttagcatc gtacgttttt tgatgcaatt aaaaagcatt tatcattttc
      481 cttatttatt ttttcttaaa tggtttagct attttctttt aatccgataa gtagcccaat
      541 catcaaggtt acaaatacta ttgcttgaat ataaatcata tgctcacctc tttataaata
      601 acttagcaat ataaagaaaa aaacgcacga acaacatagg aatataaaca aacgtttttg
      661 tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa aagcagaaag atactcaaaa
      721 taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa ggtaatttgt tattataaaa
      781 taaagaaaaa acaaaataaa aataaagaaa agagggaatg aaatggtaaa agcagaagta
      841 ggaagatctt atatatgcaa accgatcgga gtatctcatg aactaatcgg ctgtattgaa
      901 cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt atgaccaacg agactatcaa
      961 cttatcaatg aaagcaagta tcgcatgctc gtaaaattct gtgatattag cgcacctgct
     1021 gaattggttt cttaaacagt atttttgaga attcgcaaga actcccttat aatataaata
     1081 aagacagaag ggagcgagaa gatatgccaa aaagaaaaag ttcagggaaa atgggaatta
     1141 ttcttgcagt gatctctgca attcctgcta ttttagctat tgttcgtgcg atgaaggaat
     1201 caagagaaag ttaagcacac attatgagcg tcaaacaaaa a
//
LOCUS       DACCZY010000249         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCZY010000249 DACCZY010000000
VERSION     DACCZY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.258"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(231..1046)
                     /gene="thiD"
                     /locus_tag="IVZ67_002895"
     CDS             complement(231..1046)
                     /gene="thiD"
                     /locus_tag="IVZ67_002895"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8166896.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          326 a          270 c          201 g          434 t
ORIGIN      
        1 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaacttg
       61 ttccttgtga ctcggagctg gacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      121 agcttgcatc tgcggtaata agtccaacca agttaaaaac ataagaagct gtttttactt
      181 ggctgttctt attacttgat gcagggcaac tttttcacaa cttctttctt ttactctaat
      241 tcaccagctg cccaatgatt gatggggcca tatttgtgcc ctacatttat ttcttcacga
      301 attgcacggt ttacatagtc ttttgccaat cggatcgctt gttcaactgt ttttcctttc
      361 gctaactcag ctgcaataca tgcagatagc gaatcgccag ttccgttgat ccggtcggta
      421 ggataaaatg gtgcatttaa ccaaaatgat tttcctgatt cgaacaatac gtagtcattg
      481 actgtttctt gctgcgcatt tttagcatga tgtcctttga tcataacgtt ttttgcaccc
      541 atatcttgca gttcttttgc tgcttgcaca aaatcttctt cagctgctaa tgtccgtcca
      601 gtcagctttt ctgcttcata aaaattaggt gtcagtactt cggccaaagg gacaagcttt
      661 tctttcaagc tttgcaaagc agattcttct agtaaaagat ttccatgttt cgttacgatt
      721 acgggatcca cgactaatgg tccaaaatca tatttttggt aattttttac aactgtctca
      781 atcaactttg aatctgctag catacctgtt ttagctgcac gtatttgaaa gtccttcgca
      841 atcagttcaa attctttgtc gataaaatct gttggcaatg tcacacttgc tccgattccg
      901 taagaattac ctgcaacaca tgcggtcatg acagaaacac cgtaaacatt tcttgtaaag
      961 aatgtatgca tatctgcttg catccctgcg cttccatcgc tatctgatcc tgcgatcgtc
     1021 attgcttgta catactgatc ttccatcaaa tcgattcctc catttttata aaaagatttt
     1081 tcataatact gttactgttt ctatcatatg tttttcaaag tatccctcag taaatatacc
     1141 actacctttt cttggtaatc aaatttattt gaaaaaacat ttgcaataaa tcagaaaata
     1201 gtggtcgttt atttgtattc gctattctaa t
//
LOCUS       DACCZY010000250         1211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCZY010000250 DACCZY010000000
VERSION     DACCZY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.259"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            67..1023
                     /locus_tag="IVZ67_002896"
     CDS             67..1023
                     /locus_tag="IVZ67_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166897.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1077..>1211)
                     /locus_tag="IVZ67_002897"
     CDS             complement(1077..>1211)
                     /locus_tag="IVZ67_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8166898.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          206 g          399 t
ORIGIN      
        1 acagggaatc ctaaaaacat tcggcttttt ttatataatc tttttaactc aacagagata
       61 agttttgtgg aattgtatac gctacctata attataaaaa cagaaaaaat gttaaaagat
      121 ttaaaacagt attatcctct gtctgcttat gaaaaaaaga aattattcca ctttttagca
      181 atcaacctgt tggctgaaca agctgattct ttagatattg ctagcaatca gtcgctattt
      241 ttttcggata taggatttta ccacttaaaa ggagtattag gagaaaaatc cgttattttt
      301 atcaataaga cactctcttg gctttatctt catgataaat tggatcaaaa atacttgaga
      361 aaaaatgaag atgaaacaat cgaatggttg aatgaacgat tcattgaaag ttttgaaaat
      421 cagtttaatc tgttgcgaga agagacgagc caattgatcc gaagagagtt aaccaaaata
      481 cattttaaac ttttgcatta cactattaat aaagctgatg aatttccact tgatgtttct
      541 ttttttagag aaaactatcc agcattttat tgctatttga tagagtatat aaatgatcta
      601 acgtcaaaac gcaaagagtt gcttcaagat aaattttttc tttttttcag ttatttatta
      661 ttattgatca atcacatacc cattcaactg atttctgaac cagtaaagat catcatcgat
      721 ttttcttatg gtgctgccta taatcaattt atcaagaaaa atttaagctt gtacattaat
      781 ctgaacacgg aagtgataga ggcaggaaag cctgagttcc cggatgttat aatcacgaat
      841 aggaataatc tatatgaaga aagtgcatct caagtagtgg tttggctcga tccgccaaga
      901 gcagtagact gggggaatct tacaaagatt ttgttgacta tccaagaaga gaaatacaaa
      961 gacagcaaaa ttgacaagca aatagacgat tttcctgaag aaatcatcga caggatcgaa
     1021 tagtgaactt cttttagaaa gtccgactgc tttttctaaa aatattattt gtcaaattaa
     1081 gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt
     1141 agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg
     1201 acttggttga a
//
LOCUS       DACCZY010000251         1211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCZY010000251 DACCZY010000000
VERSION     DACCZY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.260"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..355)
                     /locus_tag="IVZ67_002898"
     CDS             complement(59..355)
                     /locus_tag="IVZ67_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166899.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(359..1177)
                     /locus_tag="IVZ67_002899"
     CDS             complement(359..1177)
                     /locus_tag="IVZ67_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8166900.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT          342 a          254 c          166 g          449 t
ORIGIN      
        1 tagttaagct atacctaacc gcttattcca ataaaaaaag tgatcagctt taaactgatc
       61 actcaccaaa aacttcatca tacaagttat ctatcatttc accaagtaaa gctgaatctg
      121 atcgaattcc taacttttcg ctgattttag atagcttctt tctttgcgat ggttgaagcg
      181 tgaattgata atttttcttc ttttcagaac tatcactttt tagtttagct accaaagaac
      241 ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact tcaggaactt
      301 cttctacttc agcttcagcg aattttttaa atctattttc tttcacttca atcactcctt
      361 attcttgtgc cgtatcaatt atcttgttgt atagcgtaaa cgtgctttca aagaaatcct
      421 tattcttacg atagatattc ttatccttag cgaactcagc aatattctta ccttctaagc
      481 tagttgcatt aagcaattcc ttctcttgta catatccaag ataattagat aacgttggta
      541 ccaattctag gaactcacga cttgaattag tattatgttt gatcctgcta ccgatcgtag
      601 cgtatttagt ttccatcagt gtttcaccag tcttaatatc aatcaactgt tttgctagtg
      661 catcaatatc tttaccaagc ttcagcaaag cttcaaaacc atctttagat ggatcagaaa
      721 tgcctacgac aagatcagat acagcccaag cattttgagc tagtagacct gcatcattat
      781 gagtatcaat cagaattaga tcatacttct ctttaagctg ttcatagtta acaacgatcc
      841 atctaaacag tagggttctg ttgttaggca tatcacgcat ttgcatctca acttgtttta
      901 aatcttcacc tgctactaaa atatccatgt tctcatttat agctaaaggt tcagctgatc
      961 catgaagata taaatgattg attgtattct ctcttttcgc tttactgaaa gcatcaggaa
     1021 agcgttttga taggttttgc gacttatctt catcaatata cagcaccttc atacctaaat
     1081 tttcgacagc atatccacct agattgtaga ttgttgttgt tttacctgtt ccaccttttt
     1141 caatgtttcc agtaatgatt gacaacttct tttccatgat taaacctccc ttattaacct
     1201 tttgtaactc c
//
LOCUS       DACCZY010000252         1209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCZY010000252 DACCZY010000000
VERSION     DACCZY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.261"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            4..963
                     /locus_tag="IVZ67_002900"
     CDS             4..963
                     /locus_tag="IVZ67_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8166901.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          401 a          230 c          247 g          331 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcg
//
LOCUS       DACCZY010000253         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCZY010000253 DACCZY010000000
VERSION     DACCZY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.262"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..1076
                     /locus_tag="IVZ67_002901"
     CDS             <1..1076
                     /locus_tag="IVZ67_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8166902.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACCZY010000254         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCZY010000254 DACCZY010000000
VERSION     DACCZY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.263"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..386
                     /locus_tag="IVZ67_002902"
     CDS             <1..386
                     /locus_tag="IVZ67_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8166903.1"
                     /translation="VCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMMEAMAS
                     GLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNENLSRI
                     KEFEVSIVKKRIKEIYEEVIGESVK"
     gene            383..>1193
                     /locus_tag="IVZ67_002903"
     CDS             383..>1193
                     /locus_tag="IVZ67_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8166904.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVS"
BASE COUNT          466 a          138 c          236 g          353 t
ORIGIN      
        1 aagtatgtga tagaaaagag ggattctgtg gcttcagaat agatatcaaa gaattggcaa
       61 aagtttctga tattttttta tttactacat tacaagaagg aatgccaaga tctatgatgg
      121 aagctatggc aagtggattg ccatgtatag caagtaaaat ccgaggtaat gtagaccttt
      181 tagtggaagg aaaaggtgga tatcttgttc cagctaaaga tgcagaatta gtagcagagt
      241 gtttgagaga actaatcaat aataaatctc ttagaaagta tatgggaaat gagaaccttt
      301 ccagaattaa agaattcgag gttagtatcg taaaaaaaag aatcaaagaa atatatgaag
      361 aagttatagg tgagagtgta aaatgaaaaa tccaatgttt tcaataatag tacctatcta
      421 ttgcgttgaa aaatatttga aagattgctt agattcaata agaaatcaga catttaaaaa
      481 ttttgaagca atactagttg atgacggtag tcctgacgaa tgcccagaaa tatgtgacta
      541 ttattcccaa atagatggac gtttaaaagt aattcataag aaaaacggtg gaataataga
      601 tgcacggaaa acaggagtca ctagtgctat gggagagtat atagtttttc tagatggtga
      661 tgattggctt gcaaatactt atttagaaaa atatgcggaa gtaattacta agtacaatcc
      721 agatataata tgtagtgggt attttgcatc acgttccaac aaaattagaa aaagagaatt
      781 gtcttgctcg tttggatatt atgacaaaga taaaattata aatttgattt atcctaaatt
      841 aattgaggat gaacatggag agtattttag accacaatta tgggcaaaag cttttagaac
      901 tactctgtac aaggaacaac aacaagtatc tgggcaagtt agtgttggtg aagatcatgc
      961 atgtacaaaa ccatgtattt accatgcaaa ttcgatatat atgatggaaa aatgtttata
     1021 ttattacaga ataaacgaaa attctataac aaaaaaacca caaccatatt attgggaagg
     1081 accaaaggct attggagagc attttgaaaa gcagattgat atgaataagt atgattttca
     1141 agatcaagta tatagaaatg ttacacacaa tttatttaat gttgctgttt cac
//
LOCUS       DACCZY010000255         1191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCZY010000255 DACCZY010000000
VERSION     DACCZY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.264"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..383
                     /locus_tag="IVZ67_002904"
     CDS             <1..383
                     /locus_tag="IVZ67_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8166905.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IVZ67_002905"
     CDS             complement(367..960)
                     /locus_tag="IVZ67_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP8166906.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          359 a          237 c          210 g          385 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa t
//
LOCUS       DACCZY010000256         1175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCZY010000256 DACCZY010000000
VERSION     DACCZY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.265"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            285..539
                     /locus_tag="IVZ67_002906"
     CDS             285..539
                     /locus_tag="IVZ67_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8166907.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            540..857
                     /locus_tag="IVZ67_002907"
     CDS             540..857
                     /locus_tag="IVZ67_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8166908.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          404 a          195 c          239 g          337 t
ORIGIN      
        1 cggtcttcga gttaccaacg gtaaccctca taccgaacgg cattcatcac ccacctatag
       61 aggatgggcg acttctgcct aattctgtta aatggatgtt aacggaagtg tatggtgagt
      121 agttcactcg tattttgata tcgtttgtat atgttttcag cgttctaagt ggcttatttt
      181 gtccaataat aatatggagt ggaatccctt aagaaacacc gttggcaatt gttgttataa
      241 catgttataa tttattcatc aaaaactaaa aggagtgtga taacatggga aaagaactaa
      301 aggttagaaa gatcggcaat agcgtgggag tgatccttcc aagctctctt ggattaaaaa
      361 gcggagacat tatccaagcc aaacaagaag gcaacttatt catattagat actactcaaa
      421 tagcaaaaga acacgatcgc aaattgattg aagaaagttt ccaagacttt gagaaaggtt
      481 taacagtttc agaaattgaa atggtaaaag ctttcggaaa atacggctgg agtgaataga
      541 tgaaagagta cacgctagtt tattctaaaa gttttcagac cagccttgaa acgataatag
      601 aagaatggga aaatgagctt gctctttctc cagccagcat aaaaagattt gtaggtgcga
      661 tccagaaggc tttagaacta accaaaaatt ttccaactat gcatgaggaa gtttccgaaa
      721 tctatggaat ggatatccca acatatcgga tcgtgatcgg caaaagctat gccttatttt
      781 atcggatcga ccatgaaaaa aaagagatac ttgtaggacg tattttccaa tctaaacaaa
      841 tgaaactagg cttttaacaa taattaggaa aaagtcgctc atcctctaca ggtgggtgat
      901 taatgccgct cgatatggtt gttaaccgtc ggttactcga agattgatat ataagactta
      961 gtaccttttt attttgctcc atgttatggc cgaaggtcta aagatacaat cattagtcta
     1021 gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg gtgaataaga gacactgcta
     1081 ttagtgaaaa aataagatat caagtacgct ctattcccaa aaatttcgtg actttactca
     1141 tgagaggttc aagtagtata tttttaatca aaatt
//
LOCUS       DACCZY010000257         1171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCZY010000257 DACCZY010000000
VERSION     DACCZY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.266"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            28..288
                     /locus_tag="IVZ67_002908"
     CDS             28..288
                     /locus_tag="IVZ67_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166909.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(650..889)
                     /locus_tag="IVZ67_002909"
     CDS             complement(650..889)
                     /locus_tag="IVZ67_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166910.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          414 a          164 c          219 g          374 t
ORIGIN      
        1 tggaaaaaca ttaaggatgg atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa
       61 tatatttgcc aacctattgg cttgaaacaa aaagtggttg gagaagtaat cagtaaatta
      121 gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta
      181 gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa
      241 tatctatata actatagcaa caaatatgaa catactttag tttcataaag atttaaataa
      301 agtgccttca aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat
      361 tgttcatgat ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg
      421 cctggtggag aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac
      481 aaactagtag ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata
      541 tcaggatcat ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta
      601 ggagtaaacg cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag
      661 tttgtttgaa ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag
      721 aaacttttat atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat
      781 cgatactatt cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag
      841 cagtaagtaa caataaattt gataaactga taaacagaat tcttttcatt atgtacacac
      901 tccttatagt atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac
      961 tatcaagtct tttatagtat ctattagtct tagtaataca tatatggaga gggtgatatc
     1021 ccagttcaaa ccagatacat tggttaccat ctgtattaac gattatgcct ctaaacgctt
     1081 tttttatgaa aaaatttata agtgaaaacg taggtatgat attatctaga ggtaattttt
     1141 ttgggtattt aaatagttgc taggaggata g
//
LOCUS       DACCZY010000258         1163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCZY010000258 DACCZY010000000
VERSION     DACCZY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.267"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            185..1163
                     /locus_tag="IVZ67_002910"
                     /pseudo
     CDS             185..1163
                     /locus_tag="IVZ67_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          414 a          151 c          247 g          351 t
ORIGIN      
        1 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
       61 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
      121 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
      181 tcaaggcagt gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt
      241 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
      301 gattgctttt tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag
      361 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
      421 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
      481 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
      541 aattggaacg attggaacaa ctgattctgt tggtaatcct tttcctgggg cttctactcc
      601 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
      661 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
      721 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
      781 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
      841 agatgcagaa aagagagctt ggagaattta cgatattttg tgtaataacg gatttacgaa
      901 acaatctgca tgtggaatat tggggaacat tcaacaagaa acaggaggaa cttttgatcc
      961 tgatactgtt caaataggtg gaccagcata cggattagtt cagtgggatg gttcttcata
     1021 tcctcttgtt ggtccagcaa catgggatgg aaaagtttat gttcaaaact tatttaatgc
     1081 tgcaggtatt aaagaaccaa taacgagctt agatgcacaa gttcgattgc ttatttggac
     1141 atttacaaat ggacaatgga tgg
//
LOCUS       DACCZY010000259         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCZY010000259 DACCZY010000000
VERSION     DACCZY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.268"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            14..1141
                     /locus_tag="IVZ67_002911"
     CDS             14..1141
                     /locus_tag="IVZ67_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8166911.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          201 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa c
//
LOCUS       DACCZY010000260         1159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCZY010000260 DACCZY010000000
VERSION     DACCZY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.269"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            41..472
                     /locus_tag="IVZ67_002912"
     CDS             41..472
                     /locus_tag="IVZ67_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166912.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            474..611
                     /locus_tag="IVZ67_002913"
     CDS             474..611
                     /locus_tag="IVZ67_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8166913.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            648..941
                     /locus_tag="IVZ67_002914"
     CDS             648..941
                     /locus_tag="IVZ67_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166914.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            938..>1159
                     /locus_tag="IVZ67_002915"
     CDS             938..>1159
                     /locus_tag="IVZ67_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8166915.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          436 a          167 c          239 g          317 t
ORIGIN      
        1 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
       61 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
      121 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
      181 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
      241 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
      301 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
      361 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
      421 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
      481 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
      541 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
      601 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
      661 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
      721 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
      781 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
      841 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
      901 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
      961 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     1021 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     1081 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     1141 cgaagaagga ggaaacaaa
//
LOCUS       DACCZY010000261         1125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCZY010000261 DACCZY010000000
VERSION     DACCZY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.270"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..86
                     /locus_tag="IVZ67_002916"
     CDS             <1..86
                     /locus_tag="IVZ67_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8166916.1"
                     /translation="KWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            91..1038
                     /locus_tag="IVZ67_002917"
     CDS             91..1038
                     /locus_tag="IVZ67_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8166917.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          360 a          170 c          239 g          356 t
ORIGIN      
        1 ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag cttcaagata
       61 tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac cggtggggct
      121 ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt aatcgtcgtc
      181 gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat cacttttatt
      241 gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa taaatttgat
      301 tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg accagtagaa
      361 acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg caagtatcaa
      421 cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga tgaaccgact
      481 ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt tgataaattc
      541 gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg tagcgcagtt
      601 cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag tggtgtgatt
      661 tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc atttacatta
      721 tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat ccaagcatta
      781 cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg aacgggtaaa
      841 gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt agaattgaca
      901 cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga tattactaag
      961 atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac taattatttg
     1021 aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc aggctctttg
     1081 tcaataagga ctgatgagtt ttgtaaaatc aaatctggga tagaa
//
LOCUS       DACCZY010000262         1103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCZY010000262 DACCZY010000000
VERSION     DACCZY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.271"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            43..1083
                     /locus_tag="IVZ67_002918"
     CDS             43..1083
                     /locus_tag="IVZ67_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8166918.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          457 a          166 c          185 g          295 t
ORIGIN      
        1 aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga ttttaaattt
       61 tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt ctttattgtt
      121 tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct tttaaaagca
      181 agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt ttattttact
      241 tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc
      301 aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag
      361 ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat
      421 atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt
      481 atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag
      541 ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca
      601 gaacttaata ataatatatt agacactaat agacacaata tagacactga aaaagaccgc
      661 ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct
      721 acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa
      781 gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtgat
      841 atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac
      901 acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac
      961 ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca
     1021 agtgaaaaac gtttgaatta tgcaagctcc ccaagcacta ttacgaaaga ctggttagag
     1081 tgaaacttta ctgacaaagt gat
//
LOCUS       DACCZY010000263         1071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCZY010000263 DACCZY010000000
VERSION     DACCZY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.272"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1071)
                     /locus_tag="IVZ67_002919"
     CDS             complement(<1..>1071)
                     /locus_tag="IVZ67_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8166919.1"
                     /translation="IEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNGSYTTRTQI
                     LKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRK
                     LIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGT
                     THQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTD
                     RFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHY
                     HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLP
                     QSFKKKFTIFHCYQSGISNAFK"
BASE COUNT          304 a          204 c          226 g          337 t
ORIGIN      
        1 ccttaaaggc atttgaaata cccgattggt agcaatggaa gatcgtgaat ttctttttaa
       61 acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct tccgtacctc
      121 gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc aattcttcgt
      181 tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta aacagtcgat
      241 gataatgccg tttgagtggc tcccatgtcg tggaatcttt gagtaagagt ttccaatatc
      301 gcttgagccg acggtacttc ttctgatttt cagagtgtga ggttcgaaaa cgattcatga
      361 tctggacacg taattgattg aagctgcgat tgatgtgttg gacaatatga aagcggtcag
      421 ttacgatctc cgcatttgga aagactgttt taagcaactg acaatagctg gcattcatgt
      481 ccatcactag gtacttgacc tttaaacggg cttttctagg aaagtgcagg aagtattgag
      541 ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgatgggtt gtcccatcaa
      601 cacaaataaa actcatggaa ccagaaacag acttcattga tttgaactca tccatacaca
      661 acaccgtcgg aaggaagttc atacgtgact ggtaatgctt cgttgcttca cgcaagatcc
      721 gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt gattgatttt
      781 cgtacagttc catgagaatt gcctgcttca gttcctttgt tagttgatga tgttcatcaa
      841 ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa cgggtacgtt
      901 ttaattctaa aatagttaac ttttctttga ctttcaatat ctgagtgcgt gtcgtatagg
      961 aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagga gagtcatcca
     1021 attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc a
//
LOCUS       DACCZY010000264         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCZY010000264 DACCZY010000000
VERSION     DACCZY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.273"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..687
                     /locus_tag="IVZ67_002920"
     CDS             <1..687
                     /locus_tag="IVZ67_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8166920.1"
                     /translation="LEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDR
                     EKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQ
                     RELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFS
                     EDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQ
                     YRRSLELSKD"
     gene            759..>1025
                     /locus_tag="IVZ67_002921"
     CDS             759..>1025
                     /locus_tag="IVZ67_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166921.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQ"
BASE COUNT          451 a          122 c          174 g          278 t
ORIGIN      
        1 ttagagttta aacttcaaga actagaactt cattactctt atttagtgaa aattaccgaa
       61 gaaaaagaac aacaaaaagc aataaaagaa cagatgatag aagaggaaaa agttcgtaga
      121 gaaattgatc gtgaaaagaa acgtattgat aaagaacaac gtcaattcaa ttctgaaatt
      181 tccaaattaa tcgcatatat gcaaaaagct aatgcagatg tggaaaaaga attatatgca
      241 aataaaattc aagaactgga agaaaaatta aaagaattag aagttatacg tgaaaatgta
      301 ttacaaagag aattaaatac tagagccggt tatgtgtatg taattagtaa tattgggtct
      361 tttggtgaag acatatataa aattggtatg actagaagat tagaacctat ggatagaata
      421 aaagaattga gtagtgcatc ggttccattt gaatttgacg ttcatgcgat gattttttca
      481 gaggacgctc cgtctttgga aactaaattg cataatcatt ttagaaaaca agaagttaat
      541 aaaattaatc aaagaaaaga atttttcaaa gtttcactag acgaaataga gaaagttgtt
      601 ttagaaaatt ataatggaac agtaacattt acaaaattag ctaaagctga acaatataga
      661 cgatctttag aattgtcaaa agattaaata tataaaaagc cttcgggctt tttcttttaa
      721 caattaatag gaacgtatat tcgaaaggaa tagaaataat gtggttagaa aaaataaatg
      781 acaaaaaatt taagtacacc gaacgttata cggatccctt aactgaaaaa aagagaaaag
      841 tgtccgttac tttatctagc aattcacgcc aagcttggaa tcaagctaac ttacttttga
      901 atgaaaaaat cgcagaaaaa ataaaacgaa atgaagaatt accactaact tttggagaat
      961 taaaaactaa atgggatgaa aaatacaagc caactgtaaa agaaagttcc tatcgaacaa
     1021 ctcag
//
LOCUS       DACCZY010000265         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCZY010000265 DACCZY010000000
VERSION     DACCZY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.274"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IVZ67_002922"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IVZ67_002922"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IVZ67_002923"
     tRNA            203..276
                     /locus_tag="IVZ67_002923"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IVZ67_002924"
     tRNA            316..405
                     /locus_tag="IVZ67_002924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IVZ67_002925"
     tRNA            420..491
                     /locus_tag="IVZ67_002925"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IVZ67_002926"
     tRNA            507..579
                     /locus_tag="IVZ67_002926"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IVZ67_002927"
     tRNA            589..661
                     /locus_tag="IVZ67_002927"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IVZ67_002928"
     tRNA            697..772
                     /locus_tag="IVZ67_002928"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IVZ67_002929"
     tRNA            774..854
                     /locus_tag="IVZ67_002929"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IVZ67_002930"
     tRNA            861..934
                     /locus_tag="IVZ67_002930"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IVZ67_002931"
     tRNA            937..1009
                     /locus_tag="IVZ67_002931"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          240 a          228 c          272 g          270 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc
//
LOCUS       DACCZY010000266         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCZY010000266 DACCZY010000000
VERSION     DACCZY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.275"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..588
                     /locus_tag="IVZ67_002932"
     CDS             <1..588
                     /locus_tag="IVZ67_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8166922.1"
                     /translation="NIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGT
                     DYRNNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYI
                     LRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDM
                     YEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            589..>1003
                     /locus_tag="IVZ67_002933"
     CDS             589..>1003
                     /locus_tag="IVZ67_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8166923.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDV"
BASE COUNT          383 a          109 c          180 g          331 t
ORIGIN      
        1 aatatttatg ttgctgctcc actgaggtat aaaaatatca tggaagttgt tcgtaatagt
       61 attcaaagta aaaaagtata ttctgtaaga gctatttgtt ctacttatct tcctgattgg
      121 agaaaaggta ctgattatag aaataattat tctgccttta aatctatggg gggaggagta
      181 gaattagact gtatacatga attggattat attataaacc tatttggttt ccctaaatca
      241 gtaaaaaaac agtttggtaa atattcgaat cttgaaatta acagtaatga tttagcaaca
      301 tatatattga gatacgatga taaaattata gaaattcatt tagattattt tggaagagtt
      361 cctacaagaa aatttcaatt gtttacttta gatgaatgta tcaatgttga cttattaaat
      421 aataaaataa gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc taatgatatg
      481 tatgagaagg agttgaaata tttctttgaa aacgtagtaa caggcaaaga aaactggaat
      541 gatttatatc atgcaaacga agtattgaaa atagctgagg agaaataaat ggaaatttta
      601 tttactattt gtggtagagc tgggtctaag gggtttaaaa ataaaaattt gaaatatttg
      661 gcaggaaagc ccctagtcca tttcgcttta gcaatgatag atatctatca acaaaaacat
      721 cctgaacata attgcacgac ttgtttgaat acggacagta atgagttagt agaattgacg
      781 actaagttaa atccgtctgt gaaaattgta gaacgtaaac ctgaattagc tacagatact
      841 gtagggaaaa ttgaagtgat acgtgattca ttcttacaac tagaaagaga agataatata
      901 tttgatttaa ttatagattt agatttgact tcacctttac gtgaagtttc taatcttgaa
      961 gaattaatag atgaacatct aaaaaattta gataaagacg tct
//
LOCUS       DACCZY010000267         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCZY010000267 DACCZY010000000
VERSION     DACCZY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.276"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            370..1001
                     /locus_tag="IVZ67_002934"
                     /pseudo
     CDS             370..1001
                     /locus_tag="IVZ67_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DACCZY010000268          996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCZY010000268 DACCZY010000000
VERSION     DACCZY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.277"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>996
                     /locus_tag="IVZ67_002935"
     CDS             <1..>996
                     /locus_tag="IVZ67_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8166924.1"
                     /translation="KFKLEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKE
                     ITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFF
                     KQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSK
                     KTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARV
                     NVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREY
                     LFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          381 a          160 c          189 g          266 t
ORIGIN      
        1 caaatttaaa ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat
       61 ttcaagagta ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag
      121 aataaataaa gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat
      181 gcaaagaaaa ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc
      241 agaagtgaaa aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa
      301 cttcttcaaa caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa
      361 ggaaacgaaa aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga
      421 aattcggttt cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa
      481 gaagactgga aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa
      541 agcaggttta atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa
      601 aacaaattat aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt
      661 aggatcaatt gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt
      721 tgtgaaaatg agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac
      781 atttatcaaa gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga
      841 gaatttcgct aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg
      901 cgctttcttc aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct
      961 gaaggaaacg aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DACCZY010000269          972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCZY010000269 DACCZY010000000
VERSION     DACCZY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.278"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            33..>972
                     /locus_tag="IVZ67_002936"
     CDS             33..>972
                     /locus_tag="IVZ67_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8166925.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPIS"
BASE COUNT          360 a          155 c          149 g          308 t
ORIGIN      
        1 tactggaaaa tactttgaat taaaatatta aaatggtagc aaaattaact gatgtcgctg
       61 aattagctgg agttagccca acaaccgttt cacgcgtcat taataataag ggatatttat
      121 cagaaaaaac aaaacaaaaa gttcatgaag ctatgaaaac tcttggttat aaaccaaata
      181 atttagcacg tggacttcaa ggaaagtcac ctcagcttat tggtcttatt tttcctaata
      241 ttagtaatat cttttactct gaactcattg agtatctcga aatagagtta tttaaacatg
      301 gatataaaac gattatttgc aatagtcaaa atgatcctgc taaagagaga gaatacattg
      361 aaatgcttga agccaaccaa gttgatggca taatctcatc aagccataat cttggaattg
      421 ctgattatga gcgtgtgaga gcacctattg ttgcttttga tagaaattta gctcctaatg
      481 tttccattgt ttcttcagat aactttgaag ggggaaaact tgcagctaag actttacaaa
      541 aaaatggttg tcaaaatacc attatgatta caggaaatga taacacggat tctcccacag
      601 aattacgtgc actaggtttt tcttttcaaa atccaaatgg aaaaattttc aaaattccta
      661 ataatctgtc tactatccgt cgtgaaatgg aaataaagtc aactattata aatcacaaac
      721 cagatggtat ttttgtttct gatgatttaa cagccattct cactatgaaa attgctcatc
      781 aacttaaact taatatccca gaagatttaa aaattattgg atacgatggt acctctttta
      841 ttgagaaatt tttcccacaa ttgacaacaa taagacaacc aattgatgaa attgcaagtc
      901 ttattgttga tattcttctg aaaaaaatta aaggcgaaaa gacaaataag gattatattt
      961 taccaatctc aa
//
LOCUS       DACCZY010000270          969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCZY010000270 DACCZY010000000
VERSION     DACCZY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.279"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            140..>969
                     /locus_tag="IVZ67_002937"
     CDS             140..>969
                     /locus_tag="IVZ67_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8166926.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          341 a          142 c          166 g          320 t
ORIGIN      
        1 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
       61 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
      121 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
      181 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
      241 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
      301 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
      361 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
      421 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
      481 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
      541 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
      601 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
      661 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
      721 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
      781 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
      841 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
      901 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
      961 attagttta
//
LOCUS       DACCZY010000271          948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCZY010000271 DACCZY010000000
VERSION     DACCZY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.280"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(89..162)
                     /locus_tag="IVZ67_002938"
     tRNA            complement(89..162)
                     /locus_tag="IVZ67_002938"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Ile,seq:cat)
     gene            complement(717..944)
                     /locus_tag="IVZ67_002939"
     CDS             complement(717..944)
                     /locus_tag="IVZ67_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166927.1"
                     /translation="MGFRTKRSTSSEKKWVYSQTIFYGGVISISLLSSTLYSFNVIDV
                     SMSNFISIIGILISAIITQLLLVFEEKSKNN"
BASE COUNT          343 a          149 c          156 g          300 t
ORIGIN      
        1 tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaattctaa
       61 cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc tacgaccgga
      121 cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt gccacataaa
      181 gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca tatcttggag
      241 tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg gttgagacag
      301 ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc aaggagagaa
      361 aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca ttcaaacatt
      421 tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa agaacttttg
      481 ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca caattatggt
      541 ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat taattcatcc
      601 taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca gcaggcttca
      661 cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata attgctttaa
      721 ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc agaaataagg
      781 attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa agaataaagt
      841 gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg tgaatatacc
      901 cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccct
//
LOCUS       DACCZY010000272          937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCZY010000272 DACCZY010000000
VERSION     DACCZY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.281"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            63..755
                     /locus_tag="IVZ67_002940"
     CDS             63..755
                     /locus_tag="IVZ67_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8166928.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          411 a          144 c          157 g          225 t
ORIGIN      
        1 acattgtgtt tgagatcact tacttttagc atgtaaaagt attggattaa ggagctatgc
       61 aaatgaaaaa gaaatttatt actggtggga ttgtcgttgc atttctatgt ctaattggct
      121 acacaggcac gcggtacgca aatgaaatta ttttgtgggg tggagagaca gatattgata
      181 caatcaatga gaatctgaca cagcttgatc atacattgac agataaagag caagcgatta
      241 cagatcttac cgctcgacta gcacaaaaag aacagacatt gcaagattca aaggcagatg
      301 tccaagaata ccaaacaaaa gtaaatgaat tagaaaacca aaaaaatcaa tcggcagcag
      361 aaaaacaaaa cttagaaagt caattagtca gcaaggatgc acaactacaa agcaaacaaa
      421 atgaaatcaa tgtcaaacta gatgagatca accaaaaaaa tgatgaaatc aatcatctga
      481 atgcagattg gacagcaaga ctacaagaag cacaagcaaa actttcagaa ttacaaaata
      541 ctgtaaatgg tttgaatcaa caaatcgcaa cactgacaca agaaaaaaca aatgtagaaa
      601 ctcagttaac agatacacaa aagaaattag acgaagcaac tgctgaaaac tcaagcttga
      661 aacaatatat tcaaaagtta gaaaaagcaa aagatgaagt ggaagataca gctgataaat
      721 ctcagcaaat tgttgaagaa catttgcgta aataatcttt cttaacaagt aaacctaaat
      781 tagtaaagaa gcaacagtaa tctatactaa gtagttcagt tttggagtac aaaatgacaa
      841 aatgtacttc aaaatggact acttttttca ttttaactta cataccaaat tacatacaaa
      901 atgatttata aaatgcaatt gaaaatgata attgaat
//
LOCUS       DACCZY010000273          936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCZY010000273 DACCZY010000000
VERSION     DACCZY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.282"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..877
                     /locus_tag="IVZ67_002941"
     CDS             <1..877
                     /locus_tag="IVZ67_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8166929.1"
                     /translation="ENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRI
                     LVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKI
                     LYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHL
                     LPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNES
                     IKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKE
                     EALMDQSKKENQVTS"
BASE COUNT          343 a          161 c          150 g          282 t
ORIGIN      
        1 agagaatttt cctgcaactt ctgtgaaaga cgtacaaaaa gaattcacag agtatgaata
       61 tattgacttt tttattgatc cctctgtaga tattttgcga cctaatctgc caatttgtcg
      121 cgaacaacgc atcttagtga atattttagc tcgactttct atcacgaaaa tcgaatcgga
      181 agaaacaagc ttggagcaat acaaactttt aaaaaaagca aacgttcctc atgttcttta
      241 ttcaagagaa atggtggagt ccttttgtac tgtatttgat ttgtttatac ctgaaaaaga
      301 aaagattctt tactcattgg cattcgcacg aaataaactt tataatggat ttcttaactt
      361 caatcaacca aatacgcccc ttcctacttt catgctttat caagaacata gcgtgttgaa
      421 agaagtccaa accgccattg aacaatttta tatggatttc agagaacaaa atcaacactt
      481 gttaccagtt attttagaaa aggaaaatgt gcaatggatg gttgaagacc tagtacattt
      541 gtatgatcgc tacaaaaaac agccgcgaat tgtgattggt gtgaactata cgcgtgattt
      601 ttatatcagt aaagatctga tcgttaagat agaacaaatt ttttcaaatg aatcgatcaa
      661 aatacaaaat gattttatgg agaactgcaa catcgttatc agcgactgcc ctttgaaaca
      721 attgccttct catatcaaaa aaatctatat tttaaaagga atcatcacac cagaagactg
      781 gaaaaaagtg attatgagga tctcagaata tatctttgaa ttaaaagaag aagcactcat
      841 ggatcaatcg aaaaaagaaa atcaagttac ttcctagacc aaataagata cataaatccg
      901 aatgcttatt aacaacctcc ggatttatgt atcaag
//
LOCUS       DACCZY010000274          916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCZY010000274 DACCZY010000000
VERSION     DACCZY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.283"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            7..645
                     /locus_tag="IVZ67_002942"
     CDS             7..645
                     /locus_tag="IVZ67_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8166930.1"
                     /translation="MFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISIL
                     NSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQN
                     EINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNI
                     QNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          412 a          112 c          131 g          261 t
ORIGIN      
        1 ttagtaattt tctccggaaa gcaatatgct gataatattt tattatgggg aggggaagat
       61 aatattgaaa agatcaacaa caatttaact atattagaca cagctctaga aaataaagag
      121 caaaaaatta gtattttaaa ttcacaattg tcttcaaaca accaaactct tgaacaacta
      181 agaactaata ttgaagtttc caaacaaaaa gtggcatctt tagaaaacga caagaatcaa
      241 ttagtatcag agaaaactaa tttagaaaat caattgtcag caaaagactc tgaattgcaa
      301 ggaaaacaaa atgaaattaa tgcgaaaata gacgaaatta accaaaaagt agctgaaata
      361 aatcgattaa atagcgattg gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa
      421 gaacaatcta ctattaatag tttaaaccag caaattaatc aaatgttgag tgaaaaaaat
      481 aacattcaaa atcaactgca agctactcag caaaagctag aagaagaaaa gtcaaaaaat
      541 agtagtttga ataattacat tgaaaagctt caaaaagcta aaaatgatgt agaggatacc
      601 gcaaataaag cacaacaaat tgtaaatgaa catacaatcc aatagatata gttttaattt
      661 aacaaatggt atgtttggag ataatgagat agaatataaa tgtcataata ttatacaatc
      721 taaacaccgt ttccttttct atttttgatg acatcagtat atagctactc gaaagagtag
      781 tctttttatt attctcgctt attattgata tgaaaatttc gatttgacaa aaggtaccga
      841 ataattttgt aatatgaatt attatcaaga agtaattatg atgataacat atggaaacta
      901 gttagtaata ataaca
//
LOCUS       DACCZY010000275          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCZY010000275 DACCZY010000000
VERSION     DACCZY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.284"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..888
                     /locus_tag="IVZ67_002943"
     CDS             <1..888
                     /locus_tag="IVZ67_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8166931.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DACCZY010000276          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCZY010000276 DACCZY010000000
VERSION     DACCZY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.285"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            98..838
                     /locus_tag="IVZ67_002944"
     CDS             98..838
                     /locus_tag="IVZ67_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166932.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          330 a          164 c          184 g          225 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tat
//
LOCUS       DACCZY010000277          881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCZY010000277 DACCZY010000000
VERSION     DACCZY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.286"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            2..>881
                     /locus_tag="IVZ67_002945"
     CDS             2..>881
                     /locus_tag="IVZ67_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8166933.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQ"
BASE COUNT          299 a          119 c          162 g          301 t
ORIGIN      
        1 tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga attttgatta
       61 tgaaatatct tacaatgaag tagttactgt tactccaaga aattataagt tgaattttcg
      121 aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga
      181 ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg ggctaaattt
      241 ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga
      301 atttattcct tggattttag gacaatgctc aacagtagga gaagctaaaa aattgttaaa
      361 aaatatcaat ttagcaaata taaattatag tgacgaactt cctttatccc ctttacattg
      421 gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat
      481 atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa
      541 tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat
      601 aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc
      661 agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga
      721 gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt
      781 gtgtgatgta ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga
      841 caaaggaatc tattattatc gaacatatga agacagtcaa a
//
LOCUS       DACCZY010000278          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCZY010000278 DACCZY010000000
VERSION     DACCZY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.287"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..262)
                     /locus_tag="IVZ67_002946"
     CDS             complement(62..262)
                     /locus_tag="IVZ67_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8166934.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          282 a          149 c          136 g          304 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat aacacaagga
      421 taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa gaaaaagttg
      481 aataaccgcg attgattcac tttttgtttt cttgtttatt tgcttttatc tgcaactttt
      541 gactttaaaa tttcttaact atgaaaacct ttcaattggt ggtatactta taaatggaat
      601 ttcgttatca acgcgttcgt tgcctttcag caagtattta aagaaaggag aatggctatg
      661 acattctata acaaagatat acaatatcga gtaaccttgg atacagattt gaatctattt
      721 gtcgtttttg acaaagaaaa taccaatcat gtagcgacag gtgttacgat cgaacaagca
      781 gtgcaagaat taaaaaaatc tgcataagga gtggaaaccc tcatatggac gattctcgtt
      841 cttctcttta aacgcatcta ctggtagata a
//
LOCUS       DACCZY010000279          869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCZY010000279 DACCZY010000000
VERSION     DACCZY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.288"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(80..>869)
                     /locus_tag="IVZ67_002947"
     CDS             complement(80..>869)
                     /locus_tag="IVZ67_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8166935.1"
                     /translation="FIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVL
                     PVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEV
                     ILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDV
                     NELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVL
                     IFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          336 a          148 c          106 g          279 t
ORIGIN      
        1 ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag gagtccaagc
       61 aatgaaagct gtcttcatat taattctcct tttttaaata caagtaagaa atgcttccca
      121 aataatacca gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca tgagcctcca
      181 ccatttcatc tataaaaata gatagtataa aaattaaaac aataccaaga aacgcataat
      241 tcactttact tctaaaaaat gttttgagta aactaaagat atttaacaca tagaaagcat
      301 agatacatat aaaagcaatt ataccacttg atacaagtac ttgaagaaat ccattatgca
      361 tgtttccatg tgctcttttt agttcattca catctgtagt atcaagatct acttttttca
      421 aaaattttgc atcgctattc ccatagaaat cggtctctcc ataaccaaat aacaaagttg
      481 gtcttataac tttaagcgca gctttccaaa tcgtaaaacg accagctgaa atttcggagt
      541 tatcactaga atgctgtaat attacttctt tttctggaag tggagtttta tctgattcat
      601 ttgactctat aacttctact tttctatcaa tcgatacagg aattgttgct aaaaaatcct
      661 tagcataatt agtaccaaaa gaaaaatata ccaatagaac taataatatg cttaccatcc
      721 atttgatttt tacaggtagt acttctctaa tgctctttct aaattttgaa ttggcaaaca
      781 acaaaagaaa taatactaaa aaagaacaaa ttactacctg ggcacctcta gacgaagaaa
      841 gtgtaatata aaatatttgt acgataaaa
//
LOCUS       DACCZY010000280          858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCZY010000280 DACCZY010000000
VERSION     DACCZY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.289"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..116
                     /locus_tag="IVZ67_002948"
     CDS             <1..116
                     /locus_tag="IVZ67_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8166936.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="IVZ67_002949"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="IVZ67_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8166937.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          224 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca agtttttt
//
LOCUS       DACCZY010000281          853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCZY010000281 DACCZY010000000
VERSION     DACCZY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.290"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(377..577)
                     /locus_tag="IVZ67_002950"
     CDS             complement(377..577)
                     /locus_tag="IVZ67_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8166938.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          308 a          132 c          135 g          278 t
ORIGIN      
        1 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
       61 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      121 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      181 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      241 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
      301 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
      361 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
      421 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
      481 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
      541 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
      601 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
      661 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
      721 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
      781 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
      841 ctatgcttta tat
//
LOCUS       DACCZY010000282          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCZY010000282 DACCZY010000000
VERSION     DACCZY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.291"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>810
                     /locus_tag="IVZ67_002951"
     CDS             <1..>810
                     /locus_tag="IVZ67_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8166939.1"
                     /translation="SIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGED
                     IKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDS
                     TKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEK
                     GADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATF
                     FVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLF"
BASE COUNT          349 a           93 c          145 g          223 t
ORIGIN      
        1 tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat aacaaatagt
       61 ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg gggaaatgta
      121 aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta tgtaaaaaga
      181 ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga gactcttgcg
      241 aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac tggggttaac
      301 gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga aggaaagcat
      361 ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga aaaaaataac
      421 cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga taatgagaaa
      481 ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca taatagcggt
      541 ggagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat tcatacagct
      601 gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac attctttgta
      661 aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga tataaattgg
      721 agagaatata atttgattaa aagttcatca aattaccctg cattacaaca atctatatca
      781 ggtatttact caatttcaaa taaattattt
//
LOCUS       DACCZY010000283          809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCZY010000283 DACCZY010000000
VERSION     DACCZY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.292"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>809
                     /locus_tag="IVZ67_002952"
     CDS             <1..>809
                     /locus_tag="IVZ67_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8166940.1"
                     /translation="ESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNI
                     VMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVN
                     KRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLK
                     AKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLAD
                     TISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          309 a          101 c          155 g          244 t
ORIGIN      
        1 gaatcttttt ctatagttca caatcaggtt tattttggcg ggggaattaa tttatggtta
       61 gccaaaaaga atggaatcag acagagaatt gctcatagcc atgcaactga agatggaaaa
      121 tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt gttacaaaat
      181 gcaacagact accttgccgt tagtcaagaa gctggtgaaa gtctgtttca aaaccacccc
      241 tttgagatag ttcataatgg tattgattta gagctttata gtaaaaattc agaagctaag
      301 gttaataaga gaaaagaatt ggatatctca atgagtactt ttgttgtggg aaacattgga
      361 cgattagaga aacaaaaaaa tcaatcttat ttgttagaga tttttttaga aatactaaaa
      421 atgaattctg attcttactt gattttagta ggtgaagggt cacttagaaa taaactgaaa
      481 gccaaagcag tagaattgga tatagagaaa agagtaaaat ttttaggaga aagaaacgat
      541 attcccgagt tattatctgt gatggatgtt tttgtaatgc cttctcttta tgaaggtctg
      601 ccaatggttg gattagaagc acaagcagca aaattaaaat tagtcttagc agatactatt
      661 tccaaagata caaagttaac aaataatgtg gattttgtat cattagatga aacaccacaa
      721 gaatgggcaa aaaaagtaat aaaagagccg tatgctaata aaataacaca agaactaaag
      781 agttatgatg tttcgtacac tactgaaca
//
LOCUS       DACCZY010000284          795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCZY010000284 DACCZY010000000
VERSION     DACCZY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.293"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>795
                     /locus_tag="IVZ67_002953"
     CDS             <1..>795
                     /locus_tag="IVZ67_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8166941.1"
                     /translation="IKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYR
                     LAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFL
                     FIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGY
                     QSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLC
                     ERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDET"
BASE COUNT          308 a           86 c          160 g          241 t
ORIGIN      
        1 atcaaaccaa atatatacgg tggattggca tgtcggttaa gaaaggtttc ttatgcatta
       61 aatataaccg gattaggaac ggcatttcaa aaaaaggggg ttttgaaaag tcttattgta
      121 tttttgtata gattagcagc taaaaaatct aaggtcatat ttttcgaaaa ttttgaaaat
      181 atgaaaacat ttcagacttt acaaattgct aaacaaagta aatacaagct attaaacggt
      241 gcaggagtag atgtagaaca ctttagttat ttagaatatc ctaaagatga gcaagttcat
      301 tttttattta ttggaagaat tatgaaagag aaaggtgtag atgaactttt tgaagcaatg
      361 aaaaacttat atagtgaaga gaaaaattgt gtacttgatg tccttgggta ttttgaagaa
      421 gattacggca taaaaattga aaaatacgaa aaagagggat ggttaaaata tcatggatat
      481 caatctgata ttcgtccctt tgtagaaaga gcacattgct ttgttttgcc tagttggcac
      541 gaaggaatgg caaatactaa ccttgaatca gcagcatcag ggagaccaat tattacaagt
      601 gatatcccag ggtgtaaaga agcagttatt gaatcgaagt ctggttattt atgtgaaaga
      661 aaaaatagtt gtagtctcta taattcgatg aataaatttc taaaattatc atatgagcag
      721 cgcattaaca tgggaaaaat ggggagaaat tatatgatta aaaaatttaa taagaagaaa
      781 gttgtcgatg aaaca
//
LOCUS       DACCZY010000285          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCZY010000285 DACCZY010000000
VERSION     DACCZY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.294"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            130..498
                     /locus_tag="IVZ67_002954"
     CDS             130..498
                     /locus_tag="IVZ67_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8166942.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          276 a          128 c          115 g          258 t
ORIGIN      
        1 ttattatttg agacatctta tcatataaat atagcggtta caacattact tgtgttttgt
       61 tcgtttttta tacataacta ttgtttttct ggtaaaataa atcaaaccac ttaatggagg
      121 cgttttagaa tggaaaaatt tcagccgatc atcaatcaaa tcaaagaaaa agaacatcaa
      181 caaaagatca aagacctttt tgctaagata acagaaacgt atccccagct agacacaact
      241 gtaaaatgga accaacctat gtttactgac catggtacct ttattattgc atttagtttt
      301 gctaaaaatc atttttctat cgcaccggaa aaagcagcga ttcgtgccct tgaaaaaaat
      361 atccaagaag caggctacgt atatacggat aacgtcatca aagttccttg gaaaagtgta
      421 atcaactggg aattgattga acaattgatt tcatttaata ttgaagataa aaaaggtcat
      481 gataaatttt ggcgataaag ctttatacga ataaaccttt tactggcgag gaaactattc
      541 atcaccttat acaggttttg ttcaatccta tcctttttat catccctggt taaacactta
      601 attgacaaag tatctgctat ttttataagg agtctttctt gtgctgaaaa aaatcactga
      661 ttgaatggct ttgaattttc tacttagtaa aattattttt tcagcaattt tcctgcaaaa
      721 cttttgaata aggaaaaaat acgagaaaat atcagtgatc tttcataata ctgccca
//
LOCUS       DACCZY010000286          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCZY010000286 DACCZY010000000
VERSION     DACCZY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.295"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..222
                     /locus_tag="IVZ67_002955"
     CDS             <1..222
                     /locus_tag="IVZ67_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8166943.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..>754
                     /locus_tag="IVZ67_002956"
     CDS             403..>754
                     /locus_tag="IVZ67_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8166944.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          278 a          134 c          135 g          207 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttg
//
LOCUS       DACCZY010000287          750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCZY010000287 DACCZY010000000
VERSION     DACCZY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.296"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            2..>750
                     /locus_tag="IVZ67_002957"
     rRNA            2..>750
                     /locus_tag="IVZ67_002957"
                     /product="23S ribosomal RNA"
BASE COUNT          232 a          146 c          224 g          148 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag
//
LOCUS       DACCZY010000288          729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCZY010000288 DACCZY010000000
VERSION     DACCZY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.297"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
BASE COUNT          241 a          126 c           91 g          271 t
ORIGIN      
        1 attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc taggaaatat
       61 ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat ctctattctt
      121 tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg ccatctatct
      181 gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt tatttttcta
      241 cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca tctacttgcg
      301 cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg attttccttt
      361 tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca aaccattgaa
      421 taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga atcggttagc
      481 tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct cttcgctaga
      541 taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa actagcatta
      601 tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta caatcaaaaa
      661 aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac gatatatttt
      721 tattgtttt
//
LOCUS       DACCZY010000289          646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCZY010000289 DACCZY010000000
VERSION     DACCZY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.298"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..446)
                     /locus_tag="IVZ67_002958"
     CDS             complement(<1..446)
                     /locus_tag="IVZ67_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8166945.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          191 a          116 c           88 g          251 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatc
//
LOCUS       DACCZY010000290          641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCZY010000290 DACCZY010000000
VERSION     DACCZY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.299"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(14..400)
                     /locus_tag="IVZ67_002959"
     CDS             complement(14..400)
                     /locus_tag="IVZ67_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166946.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          200 a          122 c           78 g          241 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc c
//
LOCUS       DACCZY010000291          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCZY010000291 DACCZY010000000
VERSION     DACCZY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.301"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            1..625
                     /locus_tag="IVZ67_002960"
                     /pseudo
     CDS             1..625
                     /locus_tag="IVZ67_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DACCZY010000292          621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCZY010000292 DACCZY010000000
VERSION     DACCZY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.302"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            287..>621
                     /locus_tag="IVZ67_002961"
     CDS             287..>621
                     /locus_tag="IVZ67_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8166947.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDS"
BASE COUNT          230 a           72 c          120 g          199 t
ORIGIN      
        1 gagcgactcc tttggtaggg tgatgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaacaac
      121 acaaaatatt atagaaccct ttattctaaa ggatttcaag gtgtctgttt gcatttatta
      181 gaaaaaagaa aaagtattgg caacagaagg tgtcaccaac actagatatt atagagccat
      241 aatatgctgt ataatatcta gtgtataggc ttaagtggag gtatatatga aaatattaac
      301 tgttgaagat gataatatga taagagaggg aataagtgaa tatctttcag aatttggata
      361 tactgttatt caagctaaag atggaagaga agccttgtca aaatttaata gcgatataaa
      421 tttggttatc ttagacattc agataccttt tataaatggt ttagaagtgt tgaaagaaat
      481 tagaaagaaa agcaatttac caattctaat cttgactgca tttagtgatg aagaatataa
      541 aattgatgca tttactaatt tagtagacgg atatgtagaa aagccatttt cattgccggt
      601 cttaaaagct agaatagatt c
//
LOCUS       DACCZY010000293          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCZY010000293 DACCZY010000000
VERSION     DACCZY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.303"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            72..>610
                     /locus_tag="IVZ67_002962"
     CDS             72..>610
                     /locus_tag="IVZ67_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8166948.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRK"
BASE COUNT          182 a           87 c           84 g          257 t
ORIGIN      
        1 ttttttctgt aaaaatattt atagtttatg catttgttag tttattatat ttatttccag
       61 catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt
      121 taagtttaga atctattttt agctccccta ttaattttga aacgtttaga tcatatggca
      181 tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt
      241 ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc attactctat
      301 ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg
      361 ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg
      421 gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg
      481 actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc
      541 atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt
      601 atcggaaaaa
//
LOCUS       DACCZY010000294          607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCZY010000294 DACCZY010000000
VERSION     DACCZY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.304"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>607
                     /locus_tag="IVZ67_002963"
     CDS             <1..>607
                     /locus_tag="IVZ67_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8166949.1"
                     /translation="KKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDW
                     HPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSF
                     TLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDI
                     QLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYT"
BASE COUNT          223 a           74 c          105 g          205 t
ORIGIN      
        1 aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat
       61 atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt
      121 atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa
      181 ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat acatttggga
      241 aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg
      301 acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg
      361 ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat
      421 gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata
      481 ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg
      541 catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg gaaccccaat
      601 atacttt
//
LOCUS       DACCZY010000295          607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCZY010000295 DACCZY010000000
VERSION     DACCZY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.305"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            290..>607
                     /locus_tag="IVZ67_002964"
     CDS             290..>607
                     /locus_tag="IVZ67_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8166950.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNL"
BASE COUNT          224 a           70 c           97 g          216 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctc
//
LOCUS       DACCZY010000296          580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCZY010000296 DACCZY010000000
VERSION     DACCZY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.306"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            112..>580
                     /locus_tag="IVZ67_002965"
     CDS             112..>580
                     /locus_tag="IVZ67_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8166951.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKIND"
BASE COUNT          226 a           66 c          115 g          173 t
ORIGIN      
        1 tgagaaagaa gttagccctt gtgaccatta gtataatgtt taattttaaa atgtataaaa
       61 cagcgtataa tatgctctgg agaatatata atcgataaag aggtaggaaa tatgaggaaa
      121 ttgacctttg tgataggaat tatgggaaaa ggaggggcag aacgagtaat tgcgaatttg
      181 gcaaataatt ttattcaaga taactgggaa attgatatta taactatata tgggaatcgg
      241 caagactatg aactgaatag aaaaatacag gtacatccaa taatatgtaa aagttcgttt
      301 cgtgcattta gacctttgga acgctttaag gaacttagga aaaaaataag ggaaatacag
      361 cctgatctgg ttatttcgtt ccttgctgat gtaaatatcc atgtcattat ttcattgtta
      421 ggagtaaaac aaaaattaat tgtttctgaa cgaaatgatc cacaacggaa tccagaagaa
      481 ccatggatta ggaaaattag agattttctt tataaaaatg tagatggaat tgtttttcaa
      541 acaccagacg caaaaaaata ttttagtaag atcaatgata
//
LOCUS       DACCZY010000297          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCZY010000297 DACCZY010000000
VERSION     DACCZY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.307"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DACCZY010000298          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCZY010000298 DACCZY010000000
VERSION     DACCZY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.308"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>534
                     /locus_tag="IVZ67_002966"
     CDS             <1..>534
                     /locus_tag="IVZ67_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8166952.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DACCZY010000299          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCZY010000299 DACCZY010000000
VERSION     DACCZY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.309"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            112..306
                     /locus_tag="IVZ67_002967"
     CDS             112..306
                     /locus_tag="IVZ67_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166953.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          223 a           68 c           86 g          149 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gactta
//
LOCUS       DACCZY010000300          525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCZY010000300 DACCZY010000000
VERSION     DACCZY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.310"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            4..474
                     /locus_tag="IVZ67_002968"
     CDS             4..474
                     /locus_tag="IVZ67_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8166954.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          186 a           98 c           77 g          164 t
ORIGIN      
        1 tttatgtcta tttataattt tgaaactact ttagaagacg gcactgtcta ttcattgaaa
       61 aaatatgcag gcaaaccgat aatcattgtt aacactgcaa caaaatgcgg tctagcacct
      121 caatttaaac aattagaaga actttaccaa gcttatcaga cacaaggtct ggttatctta
      181 ggctttcctt ccaatcaatt taaacaagag ctagattcag cgactgaagc agcagaagct
      241 tgtaaaacca cttatggcgt taccttccct atgcatcaat tgattgacgt aaatggagaa
      301 aatacagatc ctctttttac ttatcttaaa aatgaagctc ctggtactgt cactaaagca
      361 ataaaatgga attttactaa atttttgatt gatagaaatg gcaaagtaat cgaacgattt
      421 gcaccacaaa cttctccatt gaaaatgacc gctgcaattg aatccgttct ttaacttaat
      481 aaaaataaaa aaagatgaac tatttctagt tcatcttttt gcctt
//
LOCUS       DACCZY010000301          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCZY010000301 DACCZY010000000
VERSION     DACCZY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.311"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            9..>481
                     /locus_tag="IVZ67_002969"
     CDS             9..>481
                     /locus_tag="IVZ67_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8166955.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          175 a           88 c           80 g          138 t
ORIGIN      
        1 cagaaattgt gcaaactcta gtcactaaag gcgatttaaa tgaattattc cgttcgcact
       61 tagaaaaagc gataaacaca ctcctacgga ctgaattaac ggctttttta gattacgaaa
      121 aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac tttcgatcaa
      181 tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat ggtgagttta
      241 aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc actattatcc
      301 atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa aaaatgtacg
      361 gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact gaagaagtaa
      421 atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg gacgctactt
      481 a
//
LOCUS       DACCZY010000302          480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCZY010000302 DACCZY010000000
VERSION     DACCZY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.312"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(71..>480)
                     /locus_tag="IVZ67_002970"
     CDS             complement(71..>480)
                     /locus_tag="IVZ67_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8166956.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          151 a           96 c           72 g          161 t
ORIGIN      
        1 cacgacctaa agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc
       61 tattcattaa ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca
      121 attcatgacg atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat
      181 acgtacgata ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt
      241 cgtattttcc ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat
      301 gaaaaaattg actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa
      361 atgtggcttt tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca
      421 tcccacgact ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttccat
//
LOCUS       DACCZY010000303          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCZY010000303 DACCZY010000000
VERSION     DACCZY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.313"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..467)
                     /locus_tag="IVZ67_002971"
     CDS             complement(<1..467)
                     /locus_tag="IVZ67_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8166957.1"
                     /translation="MVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAILK
                     KYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCAIA
                     ENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRLYS"
BASE COUNT          153 a           87 c           43 g          184 t
ORIGIN      
        1 gaatataaac gattgaaata gaattgctta aaaaattctt tcttgttata tgttgctaat
       61 tcaattgttt ttaagttcgt tagacttttt cttattttta ctctttctat ttctctatca
      121 gaaaaaatgc ctacttctcc aattgcaatg tccgcattct ctgcaattgc acaattatat
      181 aaactttcaa acatagtatt ggaaacaaaa tcatcagaat ccacaaaagc caaaaattct
      241 ccattagcta ttttgatccc tcgatttcta gttggcccta accccatatt tttatgttcg
      301 taaactttta ttctagaatc aagcttttca tatttcttta atatcgccaa cgtattatct
      361 gttgaaccat cgtttaacaa aataatctca atatctttca atgtttgatt tatcaaacta
      421 tttatacact catcaatata tttttcaaca ttataacaag gtacaat
//
LOCUS       DACCZY010000304          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.314, whole genome shotgun sequence.
ACCESSION   DACCZY010000304 DACCZY010000000
VERSION     DACCZY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.314"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
BASE COUNT          149 a           88 c           52 g          168 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatgaat
//
LOCUS       DACCZY010000305          455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCZY010000305 DACCZY010000000
VERSION     DACCZY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.315"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
BASE COUNT          135 a           72 c           64 g          184 t
ORIGIN      
        1 taacgcaatt gtttttaagg ataaatttct taagatggta catttcaaaa tggcatttga
       61 catatgaaac aaaaaagaaa gaaaagagca taagggaaaa ctgatagcac cttcctacag
      121 gaaggtggct aagttggctg tgccaactgg ttttctttca aaatcatttc ataatttttg
      181 atatcataaa aaaatccatt ttggacctat tttcggtcat aatttagtac ctacttttgg
      241 tcatagtttc gtccgtagtt ttggtcatag tgactaaggc ttttctgttc acaaaattgg
      301 tttaaatttg ctttctttaa cacttcattt atatcatagc aattataatg tttacacaac
      361 atcattccta attttaacgg ttgttcctct ttatattttt taagttctac atttttttgc
      421 gtttaattac ttttctcttt tctttatatt ccgct
//
LOCUS       DACCZY010000306          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.316, whole genome shotgun sequence.
ACCESSION   DACCZY010000306 DACCZY010000000
VERSION     DACCZY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.316"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..>453
                     /locus_tag="IVZ67_002972"
     CDS             <1..>453
                     /locus_tag="IVZ67_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8166958.1"
                     /translation="TVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPI
                     ASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEI
                     NHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC"
BASE COUNT          172 a           65 c           81 g          135 t
ORIGIN      
        1 actgtttcta gtaaaaaaaa tttcaatcaa aaaataattt ctcagccggg acatattgtt
       61 gtaaacaaag aaacagtaga ggcgtatacc actaaaggaa tggattattt tttatatgat
      121 tattctccta tcgcttcatt ggagaataag gagcatataa taaaacacga agcaaagatt
      181 ggcagtcaat gggtaaacct tccttataaa acaactggta gttctatcat atatagagta
      241 actaatgaga agagccaagt aatcacactt cctgtatatg cttatttagg aacgaatgta
      301 gaaataaatc atcaacctgt aaaggagcag gtgaatgcta atggattggt acaaataagc
      361 ggagtcaaag gagttgctga tattaaagta agcacaagtt atacagtaac gactattatt
      421 tcttatcttg ttacattaac tgcatattta tgt
//
LOCUS       DACCZY010000307          448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.317, whole genome shotgun sequence.
ACCESSION   DACCZY010000307 DACCZY010000000
VERSION     DACCZY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.317"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(33..269)
                     /locus_tag="IVZ67_002973"
                     /pseudo
     CDS             complement(33..269)
                     /locus_tag="IVZ67_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a           76 c           77 g          150 t
ORIGIN      
        1 gaaggactga ccttctgtct cagatttttt tgttatttca cttgaatcaa ttgattgatc
       61 aaaaaaatcc tgattttcat gttctcaaat gatttaaatc cataagataa tcgtttcagt
      121 gtcttgatat ggggaatctt ggcctctatt ttgccattag aatacgggta aatcatagca
      181 ttgtaaatac cttcttcata cgttaaaagg ttttgaagtt tcgtgcgaag ttgtgcatca
      241 agtgtttcag gaagattttt taataacgcc gtttgtataa ttaagcgaga caaataaaaa
      301 agccatttat gttacactcc aattgtaata cccgctaaag catacaagga gagtgaacat
      361 aaatgactta tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc
      421 aagaaactcc ggttgctatc gttgcgaa
//
LOCUS       DACCZY010000308          434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.318, whole genome shotgun sequence.
ACCESSION   DACCZY010000308 DACCZY010000000
VERSION     DACCZY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.318"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
BASE COUNT          152 a           74 c           68 g          140 t
ORIGIN      
        1 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
       61 gcataccata tttttgaaaa aaagtgagaa aaaaggctta caattggagt ggtagttaat
      121 tagtgactta tttttgattt tatagcactg atactataaa atatagatat catcatatta
      181 cacaatctta atactaactt aaaaaatatc tcctttcaca agtatggtga taaaattcgt
      241 tccgggctac ctttttaggt agcctacttt aatctttata cctttctgga tcaacgaaag
      301 tatactttat atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcatga
      361 atcggctaca ctagactaga cagaaaaaat aaggtgtgta gaataataaa aaacacactg
      421 gaggattttt catg
//
LOCUS       DACCZY010000309          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.319, whole genome shotgun sequence.
ACCESSION   DACCZY010000309 DACCZY010000000
VERSION     DACCZY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.319"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..232)
                     /locus_tag="IVZ67_002974"
     CDS             complement(<1..232)
                     /locus_tag="IVZ67_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAP8166959.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMI"
     gene            complement(225..>427)
                     /locus_tag="IVZ67_002975"
     CDS             complement(225..>427)
                     /locus_tag="IVZ67_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8166960.1"
                     /translation="ETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKS
                     DYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          154 a           75 c           39 g          159 t
ORIGIN      
        1 taatcattgc tccaatcagt agccaagaaa aacgcatttt aaaaaaattt ttctttataa
       61 aaacctcata caaaactata attccacccc aagcaaaagc tattttcatc gggatattag
      121 ttacattaaa agtactaggc ataaaataaa gcagacaaat gattaaattt attactcgaa
      181 acgcatctcg acttaataaa ctcttctcta aaacaggtac tattctattc atctatattt
      241 ccaactttca aaatttcttt atcaattcta tttaatagtt tatcagagta atccgattta
      301 acttttctta caaataattt ttcactttct tttatttgat ctatatccct ttcatcaaac
      361 catttgttca tggaaggatc aataatatga atatttgcta atttcgaagt ttgtaaagta
      421 gtttctt
//
LOCUS       DACCZY010000310          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.denovo.320, whole genome shotgun sequence.
ACCESSION   DACCZY010000310 DACCZY010000000
VERSION     DACCZY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.denovo.320"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..390
                     /locus_tag="IVZ67_002976"
     CDS             <1..390
                     /locus_tag="IVZ67_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8166961.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DACCZY010000311        14817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.068, whole genome shotgun sequence.
ACCESSION   DACCZY010000311 DACCZY010000000
VERSION     DACCZY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.068"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            177..1073
                     /locus_tag="IVZ67_002977"
     CDS             177..1073
                     /locus_tag="IVZ67_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8166962.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1223..2164)
                     /locus_tag="IVZ67_002978"
     CDS             complement(1223..2164)
                     /locus_tag="IVZ67_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8166963.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2290..3411
                     /locus_tag="IVZ67_002979"
     CDS             2290..3411
                     /locus_tag="IVZ67_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8166964.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3669..5171
                     /locus_tag="IVZ67_002980"
     CDS             3669..5171
                     /locus_tag="IVZ67_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8166965.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5238..5765
                     /locus_tag="IVZ67_002981"
     CDS             5238..5765
                     /locus_tag="IVZ67_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8166966.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5976..7037
                     /locus_tag="IVZ67_002982"
     CDS             5976..7037
                     /locus_tag="IVZ67_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8166967.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7040..7936
                     /gene="murQ"
                     /locus_tag="IVZ67_002983"
     CDS             7040..7936
                     /gene="murQ"
                     /locus_tag="IVZ67_002983"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8166968.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7950..9932
                     /locus_tag="IVZ67_002984"
     CDS             7950..9932
                     /locus_tag="IVZ67_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8166969.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9997..10443)
                     /gene="nrdI"
                     /locus_tag="IVZ67_002985"
     CDS             complement(9997..10443)
                     /gene="nrdI"
                     /locus_tag="IVZ67_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8166970.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10654..12078
                     /locus_tag="IVZ67_002986"
     CDS             10654..12078
                     /locus_tag="IVZ67_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8166971.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12259..13032)
                     /locus_tag="IVZ67_002987"
     CDS             complement(12259..13032)
                     /locus_tag="IVZ67_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8166972.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13029..13934)
                     /locus_tag="IVZ67_002988"
     CDS             complement(13029..13934)
                     /locus_tag="IVZ67_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8166973.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14088..14408)
                     /locus_tag="IVZ67_002989"
     CDS             complement(14088..14408)
                     /locus_tag="IVZ67_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8166974.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4758 a         2628 c         3118 g         4313 t
ORIGIN      
        1 caattacaac tattcagaaa acttttaggc gaattttaat agaagaatga tttatttcat
       61 catattttca tacaaatgtt aaattttcat acaaatatag gaaacgtttt actggctttt
      121 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga
      181 acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg ccttccatcc
      241 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagcta
      301 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      361 tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt tggaatcact
      421 tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      481 tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat agttcatttt
      541 attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      601 cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata aaaagagatg
      661 ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga tatcttactt
      721 atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg ttgcgggagg
      781 gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag aaactagtga
      841 cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt tttttgcttc
      901 gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag ccaatcctat
      961 cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc actaacgatt
     1021 ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct
     1081 acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc atcatgagag
     1141 ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag
     1201 ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta
     1261 attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa
     1321 tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca
     1381 tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca
     1441 cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga
     1501 ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca
     1561 aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac
     1621 gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt
     1681 ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca
     1741 atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat
     1801 ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca
     1861 aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat
     1921 tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg
     1981 gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat
     2041 ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc
     2101 tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt
     2161 tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga
     2221 aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga
     2281 gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt
     2341 gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg
     2401 ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca
     2461 tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg
     2521 acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat
     2581 ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa
     2641 cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc
     2701 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
     2761 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
     2821 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
     2881 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
     2941 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
     3001 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
     3061 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
     3121 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
     3181 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
     3241 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
     3301 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
     3361 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
     3421 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
     3481 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
     3541 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
     3601 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     3661 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     3721 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     3781 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     3841 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     3901 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     3961 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     4021 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     4081 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     4141 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     4201 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     4261 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     4321 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     4381 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     4441 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     4501 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     4561 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     4621 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     4681 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     4741 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     4801 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     4861 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     4921 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     4981 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     5041 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     5101 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     5161 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     5221 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     5281 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     5341 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     5401 ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga
     5461 ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag
     5521 atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag
     5581 tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa
     5641 atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc
     5701 cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
     5761 agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca
     5821 gtgccttttt taggttatga caattctttt gataatataa actcaacctt tagaaatgaa
     5881 atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat
     5941 agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga
     6001 acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg
     6061 tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat
     6121 tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag
     6181 gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg
     6241 gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac
     6301 atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga
     6361 aagaagcaga agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg
     6421 aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc
     6481 tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat
     6541 tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag
     6601 caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc
     6661 aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag
     6721 aattttacga atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca
     6781 tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta
     6841 cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga
     6901 tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta
     6961 tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa
     7021 gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag
     7081 aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa
     7141 caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt
     7201 gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg
     7261 aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga
     7321 accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga
     7381 aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa
     7441 aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt
     7501 agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga
     7561 aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg
     7621 ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat
     7681 cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa
     7741 tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga
     7801 aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat
     7861 tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt
     7921 aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac
     7981 tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact
     8041 gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa
     8101 aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac
     8161 ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag
     8221 aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg
     8281 taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa
     8341 tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg
     8401 gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac
     8461 ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt
     8521 ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa
     8581 ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac
     8641 tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca
     8701 tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg
     8761 cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt
     8821 tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg
     8881 tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg
     8941 ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg
     9001 gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat
     9061 tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt
     9121 taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag
     9181 gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga
     9241 tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct
     9301 ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg
     9361 ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg
     9421 aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg
     9481 tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc
     9541 cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata
     9601 tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga
     9661 tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag
     9721 gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg
     9781 tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct
     9841 tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag
     9901 caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa
     9961 gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat
    10021 ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg
    10081 aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt
    10141 atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc
    10201 acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa
    10261 ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg
    10321 gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata
    10381 ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt
    10441 catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg
    10501 tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt
    10561 atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt
    10621 gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga
    10681 attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt
    10741 ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc
    10801 gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca
    10861 gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt
    10921 atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg
    10981 ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg
    11041 atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa
    11101 aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt
    11161 attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac
    11221 attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc
    11281 atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca
    11341 aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt
    11401 tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc
    11461 aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc
    11521 gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta
    11581 ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa
    11641 ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc
    11701 tttttacttt cattattatt cactgacagt gcttataact ttgcttacac acttggtaca
    11761 gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag
    11821 cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct
    11881 atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt
    11941 tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat
    12001 aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact
    12061 gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt
    12121 tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca
    12181 gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc
    12241 ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa
    12301 gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa
    12361 ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt
    12421 ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga
    12481 agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc
    12541 aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca aagtataagt
    12601 gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg
    12661 gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga
    12721 ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata
    12781 aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc
    12841 aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg
    12901 atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa
    12961 tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac
    13021 aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat
    13081 tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc
    13141 tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct
    13201 tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag
    13261 ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc
    13321 tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga
    13381 ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct
    13441 cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg
    13501 ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct
    13561 tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca
    13621 tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc
    13681 gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca
    13741 ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg
    13801 atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag
    13861 ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc
    13921 gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg
    13981 agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata
    14041 ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta
    14101 gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga
    14161 ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct
    14221 gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa
    14281 aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca
    14341 ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc
    14401 catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc
    14461 ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat
    14521 ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag
    14581 tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc
    14641 atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct
    14701 atatattatc gggcaataaa ttaaaaaaag ctttacctta caataagtaa gataattttt
    14761 gtgataaaaa aataacgaaa aatatttaaa aatagtgtct atttcgtgag aaaacaa
//
LOCUS       DACCZY010000312        10087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.104, whole genome shotgun sequence.
ACCESSION   DACCZY010000312 DACCZY010000000
VERSION     DACCZY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.104"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            537..1139
                     /locus_tag="IVZ67_002990"
     CDS             537..1139
                     /locus_tag="IVZ67_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166975.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="IVZ67_002991"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="IVZ67_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2344..2640
                     /locus_tag="IVZ67_002992"
     CDS             2344..2640
                     /locus_tag="IVZ67_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8166976.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2641..3057
                     /locus_tag="IVZ67_002993"
     CDS             2641..3057
                     /locus_tag="IVZ67_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8166977.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="IVZ67_002994"
     CDS             3077..4621
                     /locus_tag="IVZ67_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8166978.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="IVZ67_002995"
     CDS             4764..4988
                     /locus_tag="IVZ67_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8166979.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="IVZ67_002996"
     CDS             5003..5872
                     /locus_tag="IVZ67_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8166980.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="IVZ67_002997"
     CDS             5853..6587
                     /locus_tag="IVZ67_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8166981.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="IVZ67_002998"
     CDS             6620..7528
                     /locus_tag="IVZ67_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8166982.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="IVZ67_002999"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="IVZ67_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8166983.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="IVZ67_003000"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="IVZ67_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8166984.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9062..9250)
                     /locus_tag="IVZ67_003001"
                     /pseudo
     CDS             complement(9062..9250)
                     /locus_tag="IVZ67_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9230..9802
                     /locus_tag="IVZ67_003002"
     CDS             9230..9802
                     /locus_tag="IVZ67_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8166985.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DACCZY010000313         9849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.108, whole genome shotgun sequence.
ACCESSION   DACCZY010000313 DACCZY010000000
VERSION     DACCZY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.108"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            complement(9..881)
                     /locus_tag="IVZ67_003003"
     CDS             complement(9..881)
                     /locus_tag="IVZ67_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8166986.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1009..1578
                     /locus_tag="IVZ67_003004"
     CDS             1009..1578
                     /locus_tag="IVZ67_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8166987.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1645..2382
                     /locus_tag="IVZ67_003005"
     CDS             1645..2382
                     /locus_tag="IVZ67_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8166988.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2613..3881
                     /locus_tag="IVZ67_003006"
     CDS             2613..3881
                     /locus_tag="IVZ67_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8166989.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4368..4832
                     /locus_tag="IVZ67_003007"
     CDS             4368..4832
                     /locus_tag="IVZ67_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8166990.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4816..5445
                     /locus_tag="IVZ67_003008"
     CDS             4816..5445
                     /locus_tag="IVZ67_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8166991.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5799..7277
                     /gene="msr"
                     /locus_tag="IVZ67_003009"
     CDS             5799..7277
                     /gene="msr"
                     /locus_tag="IVZ67_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8166992.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7324..7770
                     /locus_tag="IVZ67_003010"
     CDS             7324..7770
                     /locus_tag="IVZ67_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8166993.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7969..8484)
                     /locus_tag="IVZ67_003011"
     CDS             complement(7969..8484)
                     /locus_tag="IVZ67_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8166994.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8601..9803
                     /locus_tag="IVZ67_003012"
     CDS             8601..9803
                     /locus_tag="IVZ67_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8166995.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3132 a         1737 c         2052 g         2928 t
ORIGIN      
        1 aactctactt aactgtattt ctccgttagg acatctaaaa atttacgagc agcaggagaa
       61 agaggctggt cttttttcca aatgagattc agtgaagctt gtaatggcgg atcgagtggg
      121 atgaatactt tttccgtccc ataagtattg atgatcccat ctagagctaa tgcaccaaaa
      181 gtcccgttat cgaccatcaa agaagcattg tacaataagt tatatgttcc gacgatcgat
      241 aactgatcta tgctgaaacc tagccattca gctagttgac tgtccactga cgtttgccgg
      301 gaaatgatca aaggaacagt tttcagttcc tgcggatgga tgactgtttg ctttgctaaa
      361 ggatgattcc gatgtatcaa aagtccccaa cgatcttggg ctggcagtcg aaatgactgg
      421 tatttttgtt tgtttgtcgg ttcgattacg agtccaaaat caagtaatcc gctatccagt
      481 ttgtctatga tatcatctgc attcccgcta tataaatgaa actggatatc aggatgcgcc
      541 ttgatcaacg aatgaaggct atcagaaatg aaagagattg ctttagtttc gcctccgcca
      601 atgtagattt ctccagagat gattgcgtca tttgttaaat tgttcgccgt tttttctacc
      661 aacgacaaga tttcttttcc ttttgaataa agatatcgac cagaatccgt caactgtatc
      721 tctcgggagc ctcgagtgaa aagtgtcaca ccaagttctt cttctaaatc tttcagttgt
      781 tttgagagag taggttgcgt gatatgcaga gattttgctg ctcggctgat atttttttct
      841 ctagctactg ctaaaaaata cgtcagtata cgaatatcca ttttttccct cctgttttta
      901 aaaaagcata ccactaagta tagttaaaag gaatagttaa gtatgtttta taagtattag
      961 acatcataag ccgtccgtta taaagtaagg ataaaaggag ggagcaaaat gacaagcctc
     1021 atcgaaaaac aaatcgtcaa taaagagatt cgagtaacgg atccgctctt tgaagtgatc
     1081 catcagatcc aagcggaaaa cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa
     1141 ccagcagata tccgcaaacg gctagaaaat atcattagtg acaaagtaga tgatacggtg
     1201 actgttctgc tgccttttta tacagatttc ggcaaacata ttagtatagg gaaaaatgtc
     1261 tttatcaatc gtcaagtgat gttcgttgac ttaggtggca tctgtttaga agattcagta
     1321 ttgattggtc ctcgtgtcaa cctcatcacg gtgaaccatc taaccgatcc aaaagaacga
     1381 agaggcttgt cagtaaagcc aatccacatc aagaaaaatg cttggatcgg agcaggagca
     1441 actattctgc caggcgtaac gattggcgag aatgccattg ttgcagcgaa tgcaactgtt
     1501 acgaaagatg tcccagacaa taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct
     1561 gtcgaacgga ttatttgaac agtaagatga aataagagat agtgagtata agcaagagat
     1621 ttttcaaaac aggaggagaa tagaatgaat gatttatcag aaaaagtgat tgtcatcatg
     1681 ggcgcttcaa gcggaatcgg tgaagcaaca acgaagctat tagcagaaaa aggggctaaa
     1741 ctagttatag ctgcacgccg agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa
     1801 gctgagctat atattcaaac agcagatgta agagattttg cacaagtcca agcagttatc
     1861 gatttagcga tggaaaagtt cggtcgaatc gatgtattgt acaataatgc aggaatcatg
     1921 ccaaccgctc cattagttga aggtcaccgt gatgaatggc aaaatatgct tgatatcaac
     1981 atcatgggtg tgttaaatgg aatttccgcc gtacttccaa tcatggaaaa acaaaaatct
     2041 gggcatatca tttcaactga ttctgtggcg ggacatgttg tttatcctga ttccgccgtt
     2101 tattgcggaa caaaatttgc tgtccgtgca atcatggaag gattacgtca agagcaacgg
     2161 caaaataaca tcaaatctac gatcatctct ccaggagctg ttcaaactga attatatcaa
     2221 accatcagcg acaaaaaggc tgctcttgaa cttcatgaag cgcaaaaaga atggggatta
     2281 acatctgaag atatagcttc tgctgtagca tttgcgatag aaacgccaga ccggatgtct
     2341 gtcagcaaca tgattatccg tccgacgaca caggaagtat aatccataac ggataatatc
     2401 cagagagaat tacttgttaa atagttattc aaacgaccat caatttaagg cgtgaatgga
     2461 gaagaatatt cttcatttac gtctttttat gcagaaaaag caaatttatt aaaaagaaac
     2521 aaatgttatt taaatatgga cattgacttg cttttacttt tttagttaca ctgttattta
     2581 ggaaaagttt cataattcaa aggagaaaaa agatgaaagc aaagaattta ttatttagcg
     2641 ttattctttt aggaaatcta gcagttgcca acacaacagt gctagctgaa gaatcgacag
     2701 aaactgtcgc aactcagaca tcgagttctt cagaaacagg aattgaaaac gatacagaaa
     2761 gtactttgca gtcagctact gtctcagaaa atacaatttc aaccgaagaa ccaacaactg
     2821 aaacaacaga acaaacacaa accgaagaca gtacatcagc agaagctcag aacaatccag
     2881 agaatgcaga agaaatcacg aaccaattga cagatgggca aacattgaca catacccatc
     2941 aagcagattt gtctgaattc gaaaactata ctggctatgc tttttatgct cgctcatctg
     3001 ctaattctca acaagcgttc attgatagta ttgcctcaac tgcacaaagt ttggcaagtg
     3061 ccaatgactt atatgcatca gtcatgattg ctcaagccat cgttgaaagt ggctggggga
     3121 acagtacttt ggcttctgcc cctaattaca atctatttgg gattaaagga agctataatg
     3181 gccaatctgt tacgatgcct acttcggaat atgtgaatgg gcaatggatt acggtgaatg
     3241 ccgcttttag aaaatatcct tcgtacaaag aatcattgca agataacgta acggtattga
     3301 agacgacttc ttttcagcca ggcgtttact actattccgg tgcatggaaa agcaatacga
     3361 actcttacaa agatgctacg gcttggctaa caggtcgtta tgccactgcg cctaattatg
     3421 gttctacatt gaacaatgta atcgaaacct ataatctgac acagtatgat acagcaccaa
     3481 cttcaacttc tccgatgtat cgtctgtata accgtcatac aggagaacat ttatatacat
     3541 tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg ggaatatgaa gggattgctt
     3601 ggcaagcacc aaacagcgga caacctgtct accgtttata caatccttac agtggagacc
     3661 atcattacac aatggcacaa tctgagatca actctttaac aaaaatcggt tggcgttatg
     3721 aaggtctaag tttttattct ggtggttcca agccaattta tcgactattt aatccaaatg
     3781 aaaaaactgg tacccatcac tataccttga gtgctaagga aagagattgg ctaacaccga
     3841 tgggctggag atatgaagga ataggatttt atggttatta aaaagaggta gtgaaaaagc
     3901 tgccctgcat caagtagtaa gaacagccaa acaaaaacag attttcatgt tttttgtttg
     3961 gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga
     4021 caacatcttg tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt
     4081 cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg
     4141 cagaagagct atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct
     4201 ttctttatca ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt
     4261 gagagcgttt tttttattac acagctgatt ctttgacttt tttctttttt tattctatta
     4321 ttgacaagtc aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag
     4381 agattagtca ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg
     4441 aagaaaatat cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa
     4501 atccattgct acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc
     4561 gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca
     4621 accgggaagt gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa
     4681 tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac
     4741 aatttctaac acttttagat aaattgaata cccaactttg caatataaca acagaaacga
     4801 aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga
     4861 tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc
     4921 aaagaagcga cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct
     4981 gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat
     5041 gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa
     5101 gaaatctaca accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa
     5161 ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc
     5221 aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat
     5281 gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat
     5341 aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac
     5401 gagtctgttc gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac
     5461 tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg
     5521 gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt
     5581 caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca
     5641 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
     5701 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
     5761 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
     5821 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
     5881 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
     5941 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
     6001 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
     6061 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
     6121 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
     6181 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
     6241 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
     6301 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
     6361 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
     6421 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
     6481 aagtgtccag taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac
     6541 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
     6601 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
     6661 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
     6721 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
     6781 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
     6841 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
     6901 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
     6961 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
     7021 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
     7081 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
     7141 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
     7201 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
     7261 ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac
     7321 agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg
     7381 gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg
     7441 ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt
     7501 gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg
     7561 atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt
     7621 gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa
     7681 atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt
     7741 gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg
     7801 gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata
     7861 ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa
     7921 gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa
     7981 ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat
     8041 tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt
     8101 caattttttt tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag
     8161 tccagtagct gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc
     8221 tttttgcgta ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc
     8281 atcagtcaag tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata
     8341 accctgctgg tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa
     8401 aattgtgatc agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa
     8461 taatatatag gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata
     8521 tcacagtaaa gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg
     8581 atgctaatag agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa
     8641 caaatgaaga aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg
     8701 cttttatggg agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg
     8761 ctacgccagc tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga
     8821 tgctatttac aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat
     8881 tgtttatcat cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga
     8941 ttgggttacg tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa
     9001 cgatcgtcag tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac
     9061 ttggctgcgg aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc
     9121 gttatccatt ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac
     9181 ttttacctga aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt
     9241 taagcaatca taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct
     9301 ttactttact tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt
     9361 ttgtattttt tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc
     9421 ttgagcggaa gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt
     9481 gtttgctgtt attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt
     9541 tagcaggatt tttccaagga attatcaata cactgttgac gacgattgct atggaaatac
     9601 caggtctgca acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag
     9661 ctttagcacc atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt
     9721 attttgcagg tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt
     9781 ttgctacaaa tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag
     9841 tcataagaa
//
LOCUS       DACCZY010000314         9494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.111, whole genome shotgun sequence.
ACCESSION   DACCZY010000314 DACCZY010000000
VERSION     DACCZY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.111"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            187..2073
                     /locus_tag="IVZ67_003013"
     CDS             187..2073
                     /locus_tag="IVZ67_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8166996.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2085..3032
                     /locus_tag="IVZ67_003014"
     CDS             2085..3032
                     /locus_tag="IVZ67_003014"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8166997.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3052..3579
                     /locus_tag="IVZ67_003015"
     CDS             3052..3579
                     /locus_tag="IVZ67_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8166998.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3642..4295)
                     /locus_tag="IVZ67_003016"
     CDS             complement(3642..4295)
                     /locus_tag="IVZ67_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8166999.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4427..5269
                     /locus_tag="IVZ67_003017"
     CDS             4427..5269
                     /locus_tag="IVZ67_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8167000.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5427..6323
                     /locus_tag="IVZ67_003018"
     CDS             5427..6323
                     /locus_tag="IVZ67_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8167001.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6326..7039
                     /locus_tag="IVZ67_003019"
     CDS             6326..7039
                     /locus_tag="IVZ67_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8167002.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7059..7577
                     /gene="msrA"
                     /locus_tag="IVZ67_003020"
     CDS             7059..7577
                     /gene="msrA"
                     /locus_tag="IVZ67_003020"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8167003.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7574..7801
                     /locus_tag="IVZ67_003021"
     CDS             7574..7801
                     /locus_tag="IVZ67_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8167004.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7953..8504
                     /gene="lepB"
                     /locus_tag="IVZ67_003022"
     CDS             7953..8504
                     /gene="lepB"
                     /locus_tag="IVZ67_003022"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8167005.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8587..9129)
                     /locus_tag="IVZ67_003023"
     CDS             complement(8587..9129)
                     /locus_tag="IVZ67_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8167006.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         3425 a         1648 c         1888 g         2533 t
ORIGIN      
        1 tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca
       61 aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca
      121 aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc aagataagga
      181 gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat
      241 ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg
      301 aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt
      361 gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct
      421 gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc
      481 caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct
      541 gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact
      601 acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa
      661 atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat
      721 gaacccgatt tactattact agacgaacca acaaaccatt tagattatga agcgatcgag
      781 tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt
      841 tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag
      901 tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta
      961 gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc
     1021 gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag
     1081 aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta
     1141 gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta
     1201 gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt
     1261 gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca
     1321 atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca
     1381 ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat
     1441 ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat
     1501 gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg
     1561 ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca
     1621 acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc
     1681 catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga
     1741 aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag
     1801 cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa
     1861 ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa
     1921 gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta
     1981 caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg
     2041 tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc
     2101 ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag
     2161 gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt
     2221 taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag
     2281 gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat
     2341 ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat tttggccata
     2401 gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg
     2461 agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg
     2521 gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag
     2581 tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc
     2641 cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc
     2701 aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc
     2761 cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag
     2821 cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac
     2881 aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg
     2941 aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac
     3001 atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct
     3061 gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt
     3121 ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga
     3181 cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg
     3241 acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg
     3301 cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat
     3361 caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat
     3421 ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa
     3481 ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag
     3541 aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc
     3601 tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca
     3661 gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag
     3721 cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc
     3781 atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag
     3841 agccacccca taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc
     3901 aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa
     3961 gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc
     4021 aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc
     4081 aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca
     4141 taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt
     4201 cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat
     4261 atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta
     4321 attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt
     4381 ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa
     4441 aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg
     4501 tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga
     4561 cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc
     4621 accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat
     4681 ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa
     4741 tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt
     4801 tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga
     4861 agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa
     4921 cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat
     4981 acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa
     5041 gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt
     5101 gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg
     5161 tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc
     5221 tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg
     5281 ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa
     5341 ataattgttt tcttttttca taaagactac ctttttgata aaatttttga taacattaga
     5401 accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt
     5461 tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag
     5521 ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac
     5581 ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag
     5641 gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg
     5701 aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa
     5761 caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg
     5821 gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta
     5881 aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc
     5941 tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc
     6001 ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga
     6061 caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga
     6121 atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc
     6181 taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag
     6241 gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt
     6301 tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac
     6361 aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc
     6421 gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc
     6481 agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt
     6541 caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa
     6601 attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat
     6661 cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc
     6721 tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa
     6781 gagaaactat aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct
     6841 taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt
     6901 agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa
     6961 caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa
     7021 aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag
     7081 gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga
     7141 tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa
     7201 cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac
     7261 aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg
     7321 atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg
     7381 ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa
     7441 aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa
     7501 agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt
     7561 ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg
     7621 cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa
     7681 gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc
     7741 gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata
     7801 agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga
     7861 attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa
     7921 gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc
     7981 gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg
     8041 gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag
     8101 gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc
     8161 aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca
     8221 gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta
     8281 aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc
     8341 taacgggaca atccaagctt ccggaagata attattttgt acttggagat aatcgaagag
     8401 tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa
     8461 gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg
     8521 cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac
     8581 gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata
     8641 aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaat
     8701 tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa aaaggctgac
     8761 tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct ttttatgata
     8821 taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag cagcttgaaa
     8881 cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc
     8941 ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga atgtgatttg
     9001 aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta ataaaagagc
     9061 agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa
     9121 agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat aaaatcacca
     9181 atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa aaaaagggtg
     9241 agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg
     9301 agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc agattagtta
     9361 tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc ttggacttct
     9421 aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt accaccattt
     9481 tcttttgcaa tttg
//
LOCUS       DACCZY010000315         7322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.132, whole genome shotgun sequence.
ACCESSION   DACCZY010000315 DACCZY010000000
VERSION     DACCZY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.132"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            <1..441
                     /locus_tag="IVZ67_003024"
     CDS             <1..441
                     /locus_tag="IVZ67_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8167007.1"
                     /translation="VLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSL
                     KDLSAPNSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVI
                     NRDERRDVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            445..648
                     /locus_tag="IVZ67_003025"
     CDS             445..648
                     /locus_tag="IVZ67_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8167008.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(948..1184)
                     /locus_tag="IVZ67_003026"
     CDS             complement(948..1184)
                     /locus_tag="IVZ67_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8167009.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(1367..1738)
                     /locus_tag="IVZ67_003027"
     CDS             complement(1367..1738)
                     /locus_tag="IVZ67_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8167010.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1735..3063)
                     /locus_tag="IVZ67_003028"
     CDS             complement(1735..3063)
                     /locus_tag="IVZ67_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8167011.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(3024..3353)
                     /locus_tag="IVZ67_003029"
     CDS             complement(3024..3353)
                     /locus_tag="IVZ67_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8167012.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3611..3981)
                     /locus_tag="IVZ67_003030"
                     /pseudo
     CDS             complement(3611..3981)
                     /locus_tag="IVZ67_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            4126..4311
                     /locus_tag="IVZ67_003031"
     CDS             4126..4311
                     /locus_tag="IVZ67_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8167013.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            5254..5913
                     /gene="vanR"
                     /locus_tag="IVZ67_003032"
     CDS             5254..5913
                     /gene="vanR"
                     /locus_tag="IVZ67_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8167014.1"
                     /translation="MSIRILFVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5916..7259
                     /gene="vanS"
                     /locus_tag="IVZ67_003033"
     CDS             5916..7259
                     /gene="vanS"
                     /locus_tag="IVZ67_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8167015.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         1816 a         1760 c         1855 g         1891 t
ORIGIN      
        1 gtgctgcagg aggcgctacg gacggagcag cacagccggg ggcgggccgt ccccggcgat
       61 ttcaaagtcc acgtcaccgc tttgcatgac agccggattg aaaccgaggc ccggcgtatt
      121 gtggacagcc tgaaagatct gtccgcgcct aacagcccca ataaaacgca tttcatggtg
      181 gagatctccc cgcactttgc ggcgattgct tccaccaagg ataatgaccg gctctttgcc
      241 atgctgccct ataaatccct gtgttttacc gggatgaaag gcaggcatgg cgtgtatgcc
      301 gtgattaacc gggacgagcg ccgggatgtg agtattcgca ggccccggcc ctctatccgc
      361 aagcagctat ccgacagcaa acaggccgcc agcccaaaaa aggcggcggc cagaaccaaa
      421 aagaatgaat tggaggtata aggaatgatg cactttaccg tggaggaaga aaacctgatc
      481 tgtatgtacc acaatgcaga ccggcgcaga acgataaaca ggctcaccgc cgtcatgccg
      541 gttatggatg gggatatgcg ggcgcaggcc cagcagaccc tttccaagct ggaacgcatg
      601 acggatgccg actatgacgg ccagaagttc cacttcacag acgaataacg aacagcagac
      661 gccgggcgcg gcggccatgt ggtcgcttgc gtccggcgtc ttttcttttt gcctattcct
      721 acgcatttag aacccggtat tgtcggggat aggatgaagt attcgcttgc ccctgcaaac
      781 atgcgggaga agccttgcag cgggcggttt tcaatccctt aaatgcgtag gttgcagcta
      841 taattagacc cctgaaacag atatagccgc gcagcgggtg tatctgtttc aggggtgtgc
      901 aggtgatagc cgcaaagcgg cgtaagaggg tgcagccctc ttgccgtcta tctctgttcc
      961 agcgggaagc tcggcaatgc ttccagcagt ttcagggaaa tgcgctgccg tatatcttcg
     1021 tcaatgactt tcttcttttt gccgtttgcc tgtctgatct ccaccgtgga aagctcgttg
     1081 atgtagtcag cataatgggc caacactttt tccgtggccc acctctcacc ggcaacggcg
     1141 gctttgatcg tttcatagtc cagcagcttt tcttgtccgt ccatcctgat acccccgtca
     1201 gactgatttt ttctatgata ccatattcgg aagcatggaa ccagagaaaa cggcgctttt
     1261 ctgctggtaa attctacgca tttagaacag cacttcggcg gggataggat aaaacaccca
     1321 cataccccgc tgaccatgcc ggaaaagcct tgaaatataa gggcttttac agctctaagt
     1381 gcgtaggttt ccagtatctt ttccgctggc gctcggcctc tttgcggcgg cgcatccgtt
     1441 tggagcaggc cgcgcagtat ttggcccggt tagacttcgg cgtgaacgtg gccccgcact
     1501 cacagcagcg tttcacttcg tcccggtctt tggtgatctc ggcatacagg gccgcatcca
     1561 gcgggaggac agcggcctta aaccagcggc acagaagcga ataggaaatg ctctgtacgc
     1621 aaatacattc ttcgccgtcg tccagcaaca ggcagttgcc gtcacaatag ttgcagcact
     1681 catgcgtcag cttccgggcc ttgcgatatt gagggtaatt cattcgtgga atactcatag
     1741 ctcttgcgtc cattcctttc ccggcgtcat acgcaaaatg ccgtccacat tctgcttgat
     1801 gatgccgtat tcctgcatct gctttttcgc ttcgccgtac ttctcataca gccgttcctt
     1861 ttcctcggcc aatcgcttgt actcggcttt cagggcgtac agatcgggga gctttttcac
     1921 accctgtccc tttagggcct ttgccgcagc ttcatacaaa atcaacgtgc tttcatgctc
     1981 acgccggaat tttgccttat ccgctgcatt gcggtattcc atagccacgg gccagagctg
     2041 cttgtaggta gaaatattct ttatcagcac cgccatatcc gcaagccggt gttccatagc
     2101 tttgagggta gcggcggcct cgtcgctggc ggcggtgatc tcggccactt tgctttccaa
     2161 atccgggtag gtgtctatcc catgttcagt caggaaattc aaagtctttg cggcctgctt
     2221 caaattgtgg agcttcgccc acctctcata gcctgccgac tgctgggcct taatgttgtt
     2281 ttccagatca atccgcaaag aaatcccctt gcgctctttg ggcgctctgg tgataacgcg
     2341 gcctttgata cgctctttga tggcttcctc ggtgtaggct tcccccaatg tcttgcagcg
     2401 ggtaaagcgt tcctgtccgg gagcgcggaa agaaataaac tttcccggct tgatctcata
     2461 gccctgcgcc tgcatgagcc gcaggaaatc atcaaaatct tttgcctgcg ggatagcggc
     2521 gtcaatggct gctttgagct tcgctttcca gctcgtccct ttccgctggg cgtcccactc
     2581 ggcatagctc ttgcccttac tctggccggg cttcaccacg gacaatccgt tttccttgca
     2641 cagcctgtcg ctggtgcgcc ggatataggc atagctctgt ttgttggaaa tgtacttgcg
     2701 ctgatttacc atatccacgg cacagaaaat aatgtgattg tggacgtggc ccttatcaat
     2761 atgggtagtc agcacatagg gatatttccc ttgcagcact tcctcggcaa gctgcttccc
     2821 gatctcatgg gcctgttccg gcgtggcttc gccgggggca aaggattgta tcaggtggtg
     2881 gcccagatta ttgccctttt ggtatgcctg cgccagcagc atttcaaatt caatgtccgc
     2941 tgtttcataa gagcaggcaa aggaggccac cagcagcttg ccgtcagttt tgtccgggtt
     3001 ctcgatgtag tcaagggctt ttttcagcgt tgacttgata ggatgtatct ttgtaactgc
     3061 catatctgcg ccagcgcccc ctttatatcc tcgatgtcct gtgcgtacac cggccccata
     3121 ccgttcagcc tctttgcaat ctgattgacg ttgacgccga tcttctgtat ctcggcggtc
     3181 tgcgcccgga tgtcgctggt gtccatgttg acgatatagc cgtcaatgag catcttccgg
     3241 gcgtaggctg aaaagttctt cgtatgcagc agcgccattt tctgttcaat catagcccgt
     3301 tcctccggcg tcacagggac gcgcagcacg atttttcgct ttctgtttgc catgcttcac
     3361 cgtcctttcc attcaggggt ttggggattt ccccaacaag caaaacggac gcggcccggc
     3421 agggacgggg caggccgttt ttccgggagt ggctacactc cctatgcttg ctacgttact
     3481 gtttctccac taacaagacg tttccaaagc cccaaactac ccgcaaaata gccctctgga
     3541 aagaaaaaag tgcaaaaaaa tagaagctac gctgtgcagc tcccgctaaa agtaaaacag
     3601 aggatgccag ttacacatcc tccgtttcat tcttcttcgg cttttcaatt agtatggcgc
     3661 tgtcaatcgc gccctcgatg atcggcaggt attcttcggg gcagagtttc agcttgtggc
     3721 tgaccctttg ccgcagttcg ctttcctccc gcatgatctc cgggttaaag tagcgttcca
     3781 caggaagccc gcaggcttta ataagctgta tgacaaccgg cagactggga atggcccgtc
     3841 gttttcgatg ttggcaagat agcgccagtc aatatttatt ttctctgcca ttgctttgcg
     3901 cgaaacgcgc tttgcttgcc gggccgcttt cacatccgcg ccgaaagttt caaagccggg
     3961 acaatcttca atattcgcca tatcgcacct cccacttaca ttgtatattt catactctgc
     4021 ccgtggaatg ttggtatatg cacctttctt aaagtttata tttcacattt tgtttctttt
     4081 attcttcgag caaaagaatt ataatgtacc ctgtggaggt gtactatgga ctacatgacg
     4141 ttgaaagagg caggcgagaa atggggtgtg cccccacgat ggataaacta ctattgtgct
     4201 gcgggacgca tccccggcgc tgtaaagatg gcgacgatct ggctcattcc caaaactgcg
     4261 gaaaagccga ttgacaggcg tacaaagaaa ggaaaggcgg ttactgaata gtataatgaa
     4321 accggcgcat tttggatatg gttttgaaga gagagaaagc atggtatttt gtcacggaaa
     4381 aactataaga tgatagatgg caggctgctc cagaccgata aaaagtattc tcatctgaaa
     4441 cagaaagaaa agatttacac atggatgtat gaagaagcga aactttatca tgacagaaat
     4501 ggaaaatgcc cgaaacaaag acgaggaagc tgctgctgta tcagccgtat atggtagaat
     4561 tgaaaaggcc ggcatctgga ttctttatgg tgaagtctcc agacattgga taactgctgg
     4621 tctaatgcgc ctttgagtat ggggtgattg attttaatac cggggaggta cggctttttg
     4681 aggaaaatat tctgggattt acatttggcg attattacat ttcctgtaac tgcaaggaca
     4741 cggatgatga agatatgagg aatatagact tgctgatttt ctactgtctt gaacggtatt
     4801 aggactgaat ctgctgcgcg gagtgcggca agagcgaccg gagggagtac acacaggccc
     4861 tgcctgtgtg aaaagccata ccgcaaggtg cattcgcaaa aaagaggccg cgcattttgc
     4921 tgaggcttgg ggtgtcccca acaagcaaaa cgagaacgcc gcaggcgggc aagtggtgga
     4981 tcagaatatc cgtaaaggat attctgatcc attgcttgcc agtattatcg ggatgttttg
     5041 acaaccgctg taatcgaaac atcccaaaca ttaagaaggt gttaattatt attatcttga
     5101 tttaattcgt ttaaaccaat aatatagata catacaccat gaaaagaagc gagtatccca
     5161 atgacggatg attaaaatcc gaaaagaaat cgctttgaaa ctacagggaa actacagact
     5221 gttatgttat cttcttaaaa tggagggatt tttatgtcga tacgaattct atttgtcgag
     5281 gatgatgatc atatctgtaa tacagtaagg gcgtttctgg ctgaggcagg atatcaggtg
     5341 gatgcctgca cagatggaaa tgaggcatac accaagtttt acgaaaacac ttatcaactg
     5401 gttattcttg atattatgct gcccggtatg aacgggcatg aacttttgcg tgaatttcgt
     5461 gcgaaaaatg atactcccat tctgatgatg acagccctgt cggatgacga aaaccaaatc
     5521 cgggcgtttg atgcagaggc agacgactat gtaacaaagc cattcaagat gcagatttta
     5581 ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc tggcaaagga aatccgtgtc
     5641 ggcaggctga cacttctgcc ggaggatttc acggtacttt gtgacggtac agagctgccc
     5701 ctgacacgaa aagaatttga aatccttttg ctgctggtgc agaacaaagg cagaacctta
     5761 acgcatgaaa tcattttgtc acgtatatgg ggatatgact ttgagggtga tggcagcaca
     5821 gtccacactc atatcaaaaa tttgcgggcc aagctgccgg aaaatatcat caaaaccatt
     5881 cgcggtgtag gataccgatt ggaggaatca taataatgga aagaaaaggg attttcatta
     5941 aggttttttc ctatacgatc attgtcctgc tactgcttgt cggtgtaaca gcaacactgt
     6001 ttgcacagca atttgtgtct tatttcagag tgatggaatt acagcaaaca gtaaaatcct
     6061 atcagccatt ggtggaactg attcagaata gcgataggct tgatattcaa gaggtggcag
     6121 ggctgtttca ctacaataac caatcctttg agttttatat tgaagataaa gagggaagcg
     6181 tactctatgc cacaccaaat gccaatacat caaatagttt tagacccaac tttctttatg
     6241 tggtacatag agatgataat atttcgattg ttgctcaaag caaggcaggt gtgggattgc
     6301 tttatcaagg gctgacaatt cggggaattg ttatgattgc gataatggtt gtattcagcc
     6361 ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc gatcaaagcc ttagcggaca
     6421 gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc gctggagcga aaggatgagc
     6481 ttggcgcact ggctcatgat atgcattcca tgtatgtcag gctgaaagaa accatcgcaa
     6541 ggctggagga tgaaatcgca agggaacatg agttggagga aacacagcga tatttctttg
     6601 cggcagcttc tcatgagcta aaaacgccca tcgcggctac aagcgttctg ttggagggaa
     6661 tgcttgaaaa tatcggtgac tacaaagatc attctaagta tctgcgcgaa tgcatcaaaa
     6721 tgatggatag gcagggcaaa atcatttccg aaatactgga gcttgtcagc ctgaatgatg
     6781 ggagaatcgt acccatagct gaaccgttgg acatagggcg cacggttgcc gagttgctgc
     6841 ccgattttca aaccttggca gaggcaaaca accagcggtt cgtcacagat attccagccg
     6901 ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc actatccaat gtcatattga
     6961 atgcagttca gaacacgccg cagggaggcg aggtacggat atggagtgag cctggtgctg
     7021 aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat tgatgatact gcgcttccaa
     7081 ggctgttcac cccattctat cgcattgatc aggcgcgaag cagaaaaagt gggcgaagcg
     7141 gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag tatgcgctgg
     7201 aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca acattgtaaa
     7261 tatttaaaac ttaaatgatt ttgggctctt tgtcaaatag gactgataga gtgagataag
     7321 aa
//
LOCUS       DACCZY010000316         6230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.140, whole genome shotgun sequence.
ACCESSION   DACCZY010000316 DACCZY010000000
VERSION     DACCZY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.140"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            40..2472
                     /locus_tag="IVZ67_003034"
     CDS             40..2472
                     /locus_tag="IVZ67_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8167016.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            2702..3769
                     /gene="rlmN"
                     /locus_tag="IVZ67_003035"
     CDS             2702..3769
                     /gene="rlmN"
                     /locus_tag="IVZ67_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8167017.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3962..5065)
                     /locus_tag="IVZ67_003036"
     CDS             complement(3962..5065)
                     /locus_tag="IVZ67_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8167018.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5617..6165
                     /gene="aac(6')"
                     /locus_tag="IVZ67_003037"
     CDS             5617..6165
                     /gene="aac(6')"
                     /locus_tag="IVZ67_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8167019.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2074 a         1071 c         1343 g         1742 t
ORIGIN      
        1 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
       61 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
      121 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
      181 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
      241 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
      301 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
      361 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
      421 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
      481 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
      541 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
      601 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
      661 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
      721 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaatatga gaagaaaaaa
      781 gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga aaatacagag
      841 ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca tcctgcgaat
      901 ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg tttttcagat
      961 gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga acaggaaaat
     1021 caagactatg aattaccgac agttgatttg cttgattcta taccaactgt ggatcaaagt
     1081 gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc
     1141 ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa attcgaagtc
     1201 cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga tatcgcgctt
     1261 gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc actgattggt
     1321 attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat cgaagcacaa
     1381 ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc tggacgggta
     1441 caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac tggtagtggg
     1501 aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa gccccatgaa
     1561 gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa cggcatacct
     1621 catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt acaaaaagta
     1681 gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct
     1741 ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca tccaacctta
     1801 ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc cagcaatgaa
     1861 gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat tcatatgatc
     1921 ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct
     1981 tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat tgattctaat
     2041 ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg tgagaataaa
     2101 ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt cgttcaattt
     2161 gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga tgaagttgaa
     2221 acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa agcacttgtc
     2281 gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat cggttacaac
     2341 cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc gtcagaagga
     2401 agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt gccggacgat
     2461 aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa
     2521 ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata cggtatgatg
     2581 tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt gaagtattgc
     2641 attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga gggaaaatac
     2701 tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt ggtttataga
     2761 aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc aaaaacgtgt
     2821 aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat tatcggagca
     2881 ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg gtacagtaaa
     2941 atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga gacaagaata
     3001 tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta cgttttgtgc
     3061 aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg cacagatcat
     3121 gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt
     3181 aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt tgcatatcat
     3241 caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca ctagtggatt
     3301 agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt
     3361 gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat
     3421 tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc gaatcacgtt
     3481 tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac aattagcaga
     3541 tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata atccagtcag
     3601 tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt atgatgtatt
     3661 gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata ttgatgcagc
     3721 ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat cgtatcctac
     3781 tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg ttcaacttaa
     3841 ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga aaaaccctca
     3901 tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt ttagcaatta
     3961 tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa gtttctacgt
     4021 caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat
     4081 ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt
     4141 cggttaacga tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat
     4201 cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa
     4261 ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag
     4321 aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa
     4381 cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac
     4441 cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt
     4501 agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc
     4561 caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg
     4621 tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga
     4681 tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg
     4741 gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg
     4801 caaacaggac gtctgctttt tggacacttt catgctggat caactgttca atatttccat
     4861 aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta
     4921 cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg
     4981 tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat
     5041 ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa
     5101 aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac
     5161 aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag tatcctaaaa
     5221 acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct cttagtattt
     5281 ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta agattattat
     5341 atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct
     5401 atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta
     5461 ttttgaataa agtgtttctg tataattttt tattttataa gtaagataaa taatgaaaga
     5521 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
     5581 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
     5641 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
     5701 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
     5761 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
     5821 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
     5881 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
     5941 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
     6001 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
     6061 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
     6121 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
     6181 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACCZY010000317         6154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.143, whole genome shotgun sequence.
ACCESSION   DACCZY010000317 DACCZY010000000
VERSION     DACCZY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.143"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            332..898
                     /gene="amaP"
                     /locus_tag="IVZ67_003038"
     CDS             332..898
                     /gene="amaP"
                     /locus_tag="IVZ67_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8167020.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            911..1099
                     /locus_tag="IVZ67_003039"
     CDS             911..1099
                     /locus_tag="IVZ67_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8167021.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1112..1669
                     /locus_tag="IVZ67_003040"
     CDS             1112..1669
                     /locus_tag="IVZ67_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8167022.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1701..1943
                     /locus_tag="IVZ67_003041"
     CDS             1701..1943
                     /locus_tag="IVZ67_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8167023.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2284..3675
                     /gene="amt"
                     /locus_tag="IVZ67_003042"
     CDS             2284..3675
                     /gene="amt"
                     /locus_tag="IVZ67_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8167024.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            4070..4543
                     /gene="dfrF"
                     /locus_tag="IVZ67_003043"
     CDS             4070..4543
                     /gene="dfrF"
                     /locus_tag="IVZ67_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8167025.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4670..5326
                     /locus_tag="IVZ67_003044"
     CDS             4670..5326
                     /locus_tag="IVZ67_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8167026.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5508..6038
                     /locus_tag="IVZ67_003045"
     CDS             5508..6038
                     /locus_tag="IVZ67_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8167027.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         2100 a          903 c         1297 g         1854 t
ORIGIN      
        1 taagaaccgt caggtttagc taagtggtga gtagttcact tctccgaaaa aatttaataa
       61 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
      121 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      181 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      241 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      301 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      361 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      421 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      481 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      541 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      601 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      661 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      721 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      781 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      841 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      901 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      961 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
     1021 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1081 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1141 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1201 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1261 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1321 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1381 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1441 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1501 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1561 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1621 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1681 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1741 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1801 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1861 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1921 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1981 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     2041 tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat aaacggtttt
     2101 ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga aaaagagcac
     2161 aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta attcaaaaaa
     2221 acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa ggaggcgaaa
     2281 aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt atgtacagca
     2341 atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc acggaagaat
     2401 aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac gattatgtgg
     2461 gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat tggaaacccg
     2521 gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca tggtgcaacg
     2581 attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac cgttcctttg
     2641 atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact tgttattttt
     2701 tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg atttttggat
     2761 cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc tggattcggc
     2821 tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga aagaagtcag
     2881 catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg ctggttcggt
     2941 tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt tggttcaaca
     3001 ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt aaaaggaaat
     3061 ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc cattacgcct
     3121 tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc gggtatcgtc
     3181 tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact tgatgtgtgg
     3241 ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt tgcatctaac
     3301 agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt gattggtgtc
     3361 atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc ggcgatgcac
     3421 tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt tttcttcttt
     3481 ggctttttgg ctatgaccat ctcaatggtc gtttcattat atgaatatcc cacattcgcg
     3541 acctcaggct tcagcttagt atttttcttg ttattaggcg gtttgctatg gtttatccct
     3601 gtggcattgt gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa
     3661 acaaaaagat attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta
     3721 ctagactaag agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc
     3781 agactatttc atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc
     3841 ttgttgcgtg agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt
     3901 tcaaaagcag aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca
     3961 aaaaaggaat ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg
     4021 cacttgatgg aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt
     4081 gattgttgcg aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat
     4141 aaagggagaa caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg
     4201 aaagtcttat gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac
     4261 cacaacagag tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattatt
     4321 attggctaaa ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt
     4381 gcaaatagta gataaaatgt atatcacaga agtagattta aatattgaag atggagatac
     4441 attctttcca gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga
     4501 ggaagtgaaa tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga
     4561 tttagggtgt tttcataaat atattgcttt ttatgcatat ataaattgtt gtaagacaaa
     4621 ttccagtttg tatggggcga taaaatcaga atttgagaga ggattgaata tgaataagga
     4681 atggtctgaa cttaataaaa caatgcaggc acaaataaaa aagaaagata cttataagag
     4741 gggtattgat actttactta ctttacgaag tcagttaata caaaccttag tatctttcaa
     4801 agaagaatta tgcagagaag attttaactc aatccccttc ataaacgctg atggttatca
     4861 tagtaagacg attgcttatt ctatttggca tattttccgc attgaagata tagttgtgca
     4921 tacagtgata aatgaagatg aacaagtatt ttttgcaggc aattatcaag agcgtatcaa
     4981 ttcacctatc attacaacag gaaatgaact catgaaacag cagattgcag acttttcaaa
     5041 acagcttaat ctggaagaat tatatttata tattttcgaa gtatgggaaa gcactgagaa
     5101 aatgcttgaa cggttatctt atgatgaatt gaaaagaaaa ataccgaaag aaagaaaagg
     5161 atacttagaa tcgttgaatg tagtaaacga caatgaaaaa gcaatttggc tgattgatta
     5221 ttggtgtaat aaagatattt gtggactaat tcaaatgccg ttttcaagac attggattat
     5281 gcacacagaa gcctgtttgc gtataaagaa caagatacat tcataggcaa aaatttcagt
     5341 ttgtagaact aaggaaattg gaatttaagg aggaataaag tatggttagt gattttataa
     5401 gagcagcatt tccatggatt ttaatgggtt tatttgtagc aatcagttgt tcccttatga
     5461 gtaaaaaaga gaaataaatt cctgtttgta tttataaaag agaaaaaatg agaaaagtag
     5521 ttttatttat tgccatgagc cttgacggat atattgcgga cggtaatggt ggagtagctt
     5581 ggctaaatgg tcatggaaat gacaatgaaa acatcgacac ttatacagaa tttaccaaag
     5641 atatagatac tgtcttaatg gggtggaata cttctcatca ggttgttact gaactttctc
     5701 cgcaagaatg ggtatataat aaatttacaa cttatgtatt aacacataaa gagtgcaatt
     5761 cttcccaaca aattcatttt acaagtgaaa atcctgtttt attattggaa cggctaaaac
     5821 aagaagcagg aaaggatatt tggatttgtg gcggagcaaa tcttgttcag cagttggtaa
     5881 gcaaaaatat gattgataaa tattacattt ctgttattcc tactctgtta ggaaatgggg
     5941 ttcgtctttt gggcaacata gacagggaaa taaagttaag gctttgtaaa acacagactt
     6001 ataacggaat aaccgatttg atttatatgc gtagataact agagagaaac agaaaagctg
     6061 ttaaaccgac ggagcgatcc gaccacgttg gcgggaaaat ccttaagatt tttcggctct
     6121 ttgtcaaatg atgttggtaa agagaaatct taga
//
LOCUS       DACCZY010000318         4819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.161, whole genome shotgun sequence.
ACCESSION   DACCZY010000318 DACCZY010000000
VERSION     DACCZY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.161"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            191..997
                     /locus_tag="IVZ67_003046"
     CDS             191..997
                     /locus_tag="IVZ67_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8167028.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1015..1842
                     /gene="vanW"
                     /locus_tag="IVZ67_003047"
     CDS             1015..1842
                     /gene="vanW"
                     /locus_tag="IVZ67_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8167029.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1839..2810
                     /locus_tag="IVZ67_003048"
     CDS             1839..2810
                     /locus_tag="IVZ67_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8167030.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2803..3831
                     /gene="vanB"
                     /locus_tag="IVZ67_003049"
     CDS             2803..3831
                     /gene="vanB"
                     /locus_tag="IVZ67_003049"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8167031.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3837..4445
                     /locus_tag="IVZ67_003050"
     CDS             3837..4445
                     /locus_tag="IVZ67_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8167032.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1360 a         1016 c         1276 g         1167 t
ORIGIN      
        1 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
       61 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      121 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      181 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      241 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      301 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      361 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      421 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      481 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      541 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      601 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      661 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      721 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      781 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      841 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      901 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
      961 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1021 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1081 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1141 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1201 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1261 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
     1321 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
     1381 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
     1441 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1501 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1561 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1621 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1681 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1741 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1801 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1861 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1921 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1981 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     2041 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2101 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2161 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2221 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2281 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2341 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2401 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2461 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2521 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2581 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2641 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2701 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2761 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2821 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2881 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2941 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     3001 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3061 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3121 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3181 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3241 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3301 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3361 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3421 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3481 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3541 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3601 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3661 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3721 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3781 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3841 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3901 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3961 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     4021 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4081 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4141 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4201 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4261 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4321 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4381 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4441 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4501 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4561 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4621 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4681 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4741 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4801 gaactattta atggactgt
//
LOCUS       DACCZY010000319         2772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.199, whole genome shotgun sequence.
ACCESSION   DACCZY010000319 DACCZY010000000
VERSION     DACCZY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.199"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            111..194
                     /locus_tag="IVZ67_003051"
     CDS             111..194
                     /locus_tag="IVZ67_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8167033.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IVZ67_003052"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IVZ67_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8167034.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="IVZ67_003053"
     CDS             complement(1319..2182)
                     /locus_tag="IVZ67_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8167035.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="IVZ67_003054"
     CDS             complement(2184..2456)
                     /locus_tag="IVZ67_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8167036.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="IVZ67_003055"
     CDS             complement(2473..2682)
                     /locus_tag="IVZ67_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8167037.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DACCZY010000320          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13768
            SAMN11027247-rid9893373.guided.300, whole genome shotgun sequence.
ACCESSION   DACCZY010000320 DACCZY010000000
VERSION     DACCZY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027247
            Sequence Read Archive: SRR10694398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743151.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 01:42:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 28 of 64
            Pseudo Genes (incomplete)         :: 31 of 64
            Pseudo Genes (internal stop)      :: 14 of 64
            Pseudo Genes (multiple problems)  :: 7 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027247-rid9893373.guided.300"
                     /isolate="UW13768"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2015"
                     /collected_by="Robert Koch Institut"
     gene            12..509
                     /gene="dfrG"
                     /locus_tag="IVZ67_003056"
     CDS             12..509
                     /gene="dfrG"
                     /locus_tag="IVZ67_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8167038.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          231 a           76 c          128 g          197 t
ORIGIN      
        1 ctgtgttata aatgaaagtt tctttgattg ctgcgatgga taagaataga gtgattggca
       61 aagagaatga cattccttgg aggattccca aggactggga atatgttaaa aatactacaa
      121 agggacatcc gataatatta ggtaggaaga accttgaatc aatcggaaga gccttacctg
      181 acagaagaaa tattattctg acgagagata aggggtttac ctttaatggt tgtgaaattg
      241 ttcattcaat agaagatgtt tttgagttat gtaaaaacga agaagaaatt tttattttcg
      301 gaggagaaca gatttataat ttgtttttcc cttatgttga gaaaatgtac atcacaaaaa
      361 tacatcatga attcgaagga gatacttttt ttccagaagt gaattatgag gaatggaatg
      421 aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc gtataactac tattttcatg
      481 tatatgaaag aaaaaactta ttgagttaac gggtgcgtta attgaagaag aacagtaaag
      541 ataaagcaca agagcttggg attactccaa gctctttttg tcatttgaaa taaattatat
      601 ctttccaatc aaattgttta tctgtaatcc ct
//
